Summary 1. Seven species or strains of Helminthosporium occurring on various cultivated grasses and one on ginger have been studied. 2. The symptoms of disease as seen in nature together with the morphology of the various forms under natural conditions have been described. 3. The eight fungi used were studied under different conditions of growth in artificial culture. The following were the main results : (a) Rate of growth depends on a number of factors, such as type of medium, amount of medium, presence or absence of light. There are certain strains, e.g. that from ginger, which show a slow growth-rate under all conditions tested. (b) The effect of temperature on growth also depends somewhat on the nature of the medium. The eight fungi show a certain amount of differentiation as regards the position of the optimal and maximal temperatures for growth and as regards sensitiveness to heating. (c) The macroscopic features of growth were found to vary very considerably. Details of the effect of cultural conditions on amount of aerial mycelium formation, colour formation, zonation, etc., are given in the text. (d) Certain microscopic growth features show a greater degree of constancy. Though amount of sporulation and spore measurements vary a good deal, spore shape and colour are markedly constant and therefore are of value for specific delimitation. 4. An extensive series of inoculation experiments was carried out with the following results: (a) Each fungus freely parasitises the host plant from which it was isolated. (b) Under artificial conditions at least, most of them are able to infect a number of hosts other than their own. (c) With the exception of two strains, all are able to produce the root-rot and foot-rot phases of disease in wheat and barley. (d) The intensity of the foot-rot and root-rot disease produced is markedly influenced by soil temperature, being greatest when the latter is in the neighbourhood of 30°C. 5. Of the eight forms studied, two have been assigned to new species. These are named Helminthosporium bicolor and Helminthosporium frumentacei . A strain from wheat is considered to be a variety of Helminthosporium halodes Drechs. and the strain from ginger to be Helminthosporium Maydis Nisikado and Miyake or a variety of the latter. The other forms are assigned to species already described. In the particular case of Helminthosporium Sacchari an amended diagnosis is given, with special reference to the size of the spores.
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