Specific Biological Activity Research Articles

Hepatocellular carcinoma and miRNAs: An in silico approach revealing potential therapeutic targets for polyphenols

BackgroundIdentifying innovative and effective therapeutic agents is imperative for treating hepatocellular carcinoma (HCC). Natural phytochemical compounds may be a feasible glimmer of hope as more than 8,000 phenolic structures are currently known, and several of them are efficient in treating different types of cancer. PurposeMicroRNAs can modulate tumor cell response to growth signals, apoptosis and replication rates, new blood vessel formation, tissue invasion, and dissemination. We disclosed herein how phenolic compounds, influencing miRNAs-regulated genes, may exert their antitumor activities. MethodsAfter a systematic review of the literature, we applied distinct in silico tools and approaches to query miRNA expression after treatment with polyphenols, determined some of the miRNA effects over target genes, elaborated protein networks and enriched their pathways, as well as presented differentially expressed genes (DEGs) found in HCC patients. Our predictions were corroborated by several in vitro and in vivo experimental studies that we presented and discussed. ResultsPhytochemicals such as berberine, curcumin, EGCG, luteolin, and quercetin are promising candidates capable of regulating different miRNAs while exerting their antitumoral effects through distinct molecular mechanisms. MiRNAs such as miRNA-122 and -34a deserve deep investigations, as they were found to be over and down-expressed by more than one polyphenol. MiRNA-regulated genes took part in molecular mechanisms such as cell death, through p53, bcl-2, and SMAD modulation; energy metabolism, by regulating PI3K/Akt pathway; antiproliferative events, mediated by Ras, c-Kit, and β-catenin; and epigenetics events, involving SIRT, HDAC, and DNMT family members. Tumor microenvironment modulation, by NOTCH1 and VEGFA, is also a potential mechanism related to polyphenols’ effects. We reported that polyphenols have specific drug ability and anticancer biological activities. Among the DEGs, 4 of them (e.g., EZH2, HRAS, STMN1, VEGFA), are candidate genes for miRNA modulation. The expression profile of gene subsets that are frequently altered in HCC patients was also characterized. ConclusionThe capacity of polyphenols to regulate miRNA actions may have a significant impact on the treatment of liver tumors; experimental and clinical studies dedicated to confirming our findings are further needed.

A Review on Procedure of QSAR Assessment in Organic Compounds As a Measure of Antioxidant Potentiality

Chemical and biological properties of substances may be inferred from their more fundamental physical, chemical, and biological characteristics using QSAR models. An insilico model may be built using QSAR to anticipate the activity of novel molecules before they are synthesised, allowing the author to establish a quantifiable link between structure and behaviour. QSAR is a powerful tool. Although QSAR modelling is a computer area, medicinal chemists are the main users and ultimate assessors, especially when it comes to developing compounds with the necessary biological activity. Several studies were conducted in which medicinal chemists and cheminformaticians collaborated to discover new compounds with specific biological activity. This was done through the development of QSAR models and their use in virtual screening, followed by experimental verification. Despite the fact that QSAR methods have their own set of limitations, their use in molecular prediction and assessment has been effective due to a division of labour in which mathematical professionals ensured the greatest quality of models. The predictions also helped experimental chemists design and test compounds that were expected to be successful. This review is being developed and implemented to look into the development of the QSAR tool in the assessment of antioxidant potentiality for diverse organic chemicals found in our environment.

Electrocoagulation pretreatment reduced the synergistic inhibition of anaerobic granular sludge by micro stickies and Ca2+ and delayed the calcification of granular sludge

The presence of micro stickies and Ca2+ in papermaking wastewater leads to a decrease in anaerobic granular sludge (AnGS) digestion performance and granular sludge calcification. This study, evaluated the effects of micro stickies and Ca2+, combined or alone, and following electrocoagulation pretreatment, on AnGS calcification and anaerobic digestion in wastewater from old corrugated container recycling. The results showed the degradation rate of organic matter, extracellular polymeric substances(EPS) content, specific methanogenic activity （SMA） and biological activity was improved noticeably via electrocoagulation pretreatment with micro stickies and Ca2+. Further, the pre-removal of micro stickies and Ca2+ not only enriched the functional microorganisms but also delayed the calcification of AnGS thereby causing a significant reduction in the damage of AnGS. Therefore, as a promising way to minimize the harmful effects of micro stickies and Ca2+, electrocoagulation pretreatment provides a new idea for delaying the calcification of AnGS.

Novel long non-coding RNA CYB561-5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non-small cell lung cancer.

Abnormally expressed long non‐coding RNAs (lncRNAs) have been recognized as potential diagnostic biomarkers or therapeutic targets in non‐small cell lung cancer (NSCLC). The role of the novel lnc‐CYB561‐5 in NSCLC and its specific biological activity remain unknown. In this study, lncRNAs highly expressed in NSCLC tissue samples compared with paired adjacent normal tissue samples and atypical adenomatous hyperplasia were identified by RNA‐seq analysis. Lnc‐CYB561‐5 is highly expressed in human NSCLC and is associated with a poor prognosis in lung adenocarcinoma. In vivo, downregulation of lnc‐CYB561‐5 significantly decreases tumour growth and metastasis. In vitro, lnc‐CYB561‐5 knockdown treatment inhibits cell migration, invasion and proliferation ability, as well as glycolysis rates. In addition, RNA pulldown and RNA immunoprecipitation (RIP) assays show that basigin (Bsg) protein interacts with lnc‐CYB561‐5. Overall, this study demonstrates that lnc‐CYB561‐5 is an oncogene in NSCLC, which is involved in the regulation of cell proliferation and metastasis. Lnc‐CYB561‐5 interacts with Bsg to promote the expression of Hk2 and Pfk1 and further lead to metabolic reprogramming of NSCLC cells.

Nutrient Availability Shifts the Biosynthetic Potential of Soil-Derived Microbial Communities.

Secondary metabolites produced by microorganisms are the main source of antimicrobials and other pharmaceutical drugs. Soil microbes have been the primary discovery source for these secondary metabolites, often producing complex organic compounds with specific biological activities. Research suggests that secondary metabolism broadly shapes microbial ecological interactions, but little is known about the factors that shape the abundance, distribution, and diversity of biosynthetic gene clusters in the context of microbial communities. In this study, we investigate the role of nutrient availability on the abundance of biosynthetic gene clusters in soil-derived microbial consortia. Soil microbial consortia enriched in high sugar medium (150mg/L of glucose and 200mg/L of trehalose) had more biosynthetic gene clusters and higher inhibitory activity than those enriched in low sugar medium (15mg/L of glucose + 20mg/L of trehalose). Our results demonstrate that experimental microbial communities are a promising tool to study the ecology of specialized metabolites.

Valorization of By-Products from Biofuel Biorefineries: Extraction and Purification of Bioactive Molecules from Post-Fermentation Corn Oil.

The aim of this work was to develop innovative and sustainable extraction, concentration, and purification technologies aimed to recover target substances from corn oil, obtained as side stream product of biomass refineries. Residues of bioactive compounds such as carotenoids, phytosterols, tocopherols, and polyphenols could be extracted from this matrix and applied as ingredients for food and feeds, nutraceuticals, pharmaceuticals, and cosmetic products. These molecules are well known for their antioxidant and antiradical capacity, besides other specific biological activities, generically involved in the prevention of chronic and degenerative diseases. The project involved the development of methods for the selective extraction of these minor components, using as suitable extraction technique solid phase extraction. All the extracted and purified fractions were evaluated by NMR spectroscopic analyses and UV–Vis spectrophotometric techniques and characterized by quali-quantitative HPLC analyses. TPC (total phenolic content) and TFC (total flavonoid content) were also determined. DPPH and ABTS radical were used to evaluate radical quenching abilities. Acetylcholinesterase (AChE), amylase, glucosidase, and tyrosinase were selected as enzymes in the enzyme inhibitory assays. The obtained results showed the presence of a complex group of interesting molecules with strong potential in market applications according to circular economy principles.

Galectin-1-Dependent Mitochondria Apoptosis Plays an Essential Role in the Potential Protein Targets of DBDCT-Induced Hepatotoxicity as Revealed by Quantitative Proteomic Analyses.

Di-n-butyl-di-(4-chlorobenzohydroxamato) tin(IV) (DBDCT), a new patent agent, exhibited strong antitumor activity. In some cases, its activity was close to or even higher than cisplatin, a first-line clinical metallic agent. Similar to platinum compounds, it also showed toxicity. However, the effective targets and mechanisms for specific toxicity and biological activity are still unclear. In this study, proteomic analysis revealed that 146 proteins (98 upregulated and 48 downregulated) were differentially identified by label-free LC-MS/MS after DBDCT treatment. Meanwhile, network analysis of these differential proteins suggested that protein Galectin-1 (Gal-1) could regulate the apoptosis process (15 related proteins), which played an essential role in the potential targets of DBDCT-induced hepatotoxicity. Furthermore, it was demonstrated that DBDCT might promote ROS production, activate NF-κB p65, inhibit Ras and p-ERK1/2 expressions, increase the level of Gal-1, subsequently upregulate the expressions of Bax, p53, Fas, and FasL, and downregulate the expression of Bcl-2. As a result of these modulations, caspase cascades were finally activated, which executed apoptosis in HL7702 liver cells. Correspondingly, NAC (inhibitor of ROS), PDTC (inhibitor of NF-κB), EGF (ERK1/2 activator), and OTX008 (inhibitor of Gal-1) were found to reverse and abolish the DBDCT-associated cytotoxicity partially. In conclusion, Gal-1 might be the potential target for toxicity and biological activity. Moreover, the present study will lay the groundwork for future research about di-n-butyl-di-(4-chlorobenzohydroxamato) tin structure optimization and developing it into a new potential anticancer agent.

Determination of the content of organic acids in the chemical composition of buckbuckthorn oil, obtained from regional raw materials

The purpose of the work is to determine the content of organic acids in the chemical composition of sea buckthorn oil obtained from regional raw materials and to establish the influence of sea buckthorn oil production technology on the content of biologically active substances. Sea buckthorn is a unique source product for enriching food products with biologically active substances that play a diverse, important role in the vital activity of the body. It has been proven that the content of organic acids and vitamin C is the main variable in the chemical components of sea buckthorn, the phytochemical and nutritional composition of whose berries differs significantly depending on the species, climatic and growing conditions, and the technology of obtaining sea buckthorn oil. This attracts attention from the standpoint of the need to study sea buckthorn, which grows in regional territories. It was established that sea buckthorn oil obtained from the juice contains: organic acids 154.1–167.5 mg%, vitamin C 168-214 mg%. Sea buckthorn oil extracted with refined sunflower oil contains: organic acids 234.5–388.6 mg%, vitamin C 189–243 mg%. The effect of the technology of obtaining sea buckthorn oil from regional raw materials on the content of biologically active substances, such as organic acids, vitamin C, was determined. Based on the results of research, the advantages of oil obtained by the technology of extraction with refined sunflower oil were determined. Such oil contains a larger amount of ascorbic acid and free organic acids. When analyzing the results of research on oil and dry raw materials collected in the Kherson region, it was established that in the case of organic acids, in particular vitamin C, the indicator of quantitative content is affected not only by the production technology, but also by the biological variety of sea buckthorn. It is advisable to intensively continue scientific work on the research of substances that are responsible for the specific biological activity of sea buckthorn oil.

Agro-industrial by-products: Valuable sources of bioactive compounds

In a world with eminent scarcity of natural resources and increasing incidence of chronic diseases related to unhealthy eating habits, the search for biologically active and environmentally friendly food products is raising among customers. Agro-industrial by-products have caught special attention from the scientific community for being an available, cost-effective and sustainable source of a wide array of bioactive compounds. Review papers frequently restrain their research to by-products derived from the production of most worldwide consumed crops. Therefore, the aim of this review is to summarize the latest overall research, which focus on the biological potential of agro-industrial by-products and their bioactive compound profile, targeting their application as food ingredients, including not only researches with worldwide consumed crops, but also local foodstuff. A total of 152 research papers, browsed in 2 databases, and involving more than 30 countries were gathered. The richness of bioactive compounds of food by-products from different industries, from fruits to marine products, is ascertained throughout this review. The diversity of food residue being investigated for their nutritional and biological capabilities and the content of specific molecules in each food group are remarkable points. Higher literature reports about fruits by-products may be explained by its wide range of bioactive compounds, especially in Latin American fruits, which includes all flavonoids subclasses, besides betaxanthins, carotenoids and phytosterols. Researchers mainly focus on the quantification of fiber, polyphenols and antioxidant capacity of the investigated by-products, obstructing the investigation of specific biological activities, which are precisely related to the main phytochemicals of the residue matrix, as each molecule has an individual mechanism of action that should be considered when evaluating its biological capabilities. Furthermore, the addition of food by-products has also been advantageous in the production of fortified or enriched bakery, dairy and meat products and functional beverages. All along this literature review, it becomes clearer the high nutritional and nutraceutical value that many by-products possess, besides their attested biological activities, such as antioxidant, anticarcinogenic, antimicrobial, among others.

A Bird's-Eye View of Proteomics.

Modern protein science is broadening horizons by moving toward the systemic description of proteins in their natural habitats. This implies a transition from a classical reductionist approach associated with consideration of the unique structure and specific biological activity of an individual protein in a purified form to studying entire proteomes and their functions. This minireview provides a brief description of structural, functional, and expression proteomics, the dark proteome (or unfoldome), and some of the tools utilized in the analyses of proteomes.

Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.

Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species. However, in equidaes, eCG exhibits only LH-like activity. To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant. We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells. We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased. The EC50 levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants decreased by 2.1-, 5.6-, and 3.4-fold, respectively, compared to that of wild-type eCG. The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells). Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR. These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.

Flavonoid/Polyphenol Ratio in Mauritia flexuosa and Theobroma grandiflorum as an Indicator of Effective Antioxidant Action.

Studies on polyphenols and flavonoids in natural products reveal benefits in the prevention of multiple diseases. Proper extraction, treatment of extracts, and quantification of polyphenols and flavonoids demand attention from the scientific community in order to report more specific biological action. Total polyphenol content (TPC) and total flavonoid content (TFC) (measured at three different times) of ethanol, methanol and acetone extracts of Mauritia flexuosa (aguaje) and Theobroma grandiflorum (copoazú) fresh pulp, from the Colombian Amazon region, were evaluated with the purpose of focusing in the polyphenol/flavonoid proportion and its effective antioxidant activity. This objective could help to explain specific flavonoid biological action based on higher flavonoid proportion rather than higher total polyphenol content. Differences in extracting solvents resulted in statistically significant different yields; the highest TPC was observed with acetone 70% in Mauritia flexuosa and ethanol 80% for T. grandiflorum. The best flavonoid/polyphenol ratio in M. flexuosa was about 1:2.4 and 1:12.8 in T. grandiflorum and the antioxidant efficacy was proportionally higher for flavonoids extracted from T. grandiflorum. HPLC analysis revealed 54 µg/g of the flavonoid kaempferol in M. Flexuosa and 29 µg/g in T. grandiflorum. Further studies evaluating this proportionality, in seeds or peel of fruits, as well as, other specific biological activities, could help to understand the detailed flavonoid action without focusing on the high total polyphenol content.

Resiniferatoxin Hampers the Nocifensive Response of Caenorhabditis elegans to Noxious Heat, and Pathway Analysis Revealed that the Wnt Signaling Pathway is Involved.

Resiniferatoxin (RTX) is a metabolite extracted from Euphorbia resinifera. RTX is a potent capsaicin analog with specific biological activities resulting from its agonist activity with the transient receptor potential channel vanilloid subfamily member 1 (TRPV1). RTX has been examined as a pain reliever, and more recently, investigated for its ability to desensitize cardiac sensory fibers expressing TRPV1 to improve chronic heart failure (CHF) outcomes using validated animal models. Caenorhabditis elegans (C. elegans) expresses orthologs of vanilloid receptors activated by capsaicin, producing antinociceptive effects. Thus, we used C. elegans to characterize the antinociceptive properties and performed proteomic profiling to uncover specific signaling networks. After exposure to RTX, wild-type (N2) and mutant C. elegans were placed on petri dishes divided into quadrants for heat stimulation. The thermal avoidance index was used to phenotype each tested C. elegans experimental group. The data revealed for the first time that RTX can hamper the nocifensive response of C. elegans to noxious heat (32 - 35°C). The effect was reversed 6h after RTX exposure. Additionally, we identified the RTX target, the C. elegans transient receptor potential channel OCR-3. The proteomics and pathway enrichment analysis results suggest that Wnt signaling is triggered by the agonistic effects of RTX on C. elegans vanilloid receptors.

A heuristic, computer-driven and top-down approach to identify novel bioactive peptides: A proof-of-principle on angiotensin I converting enzyme inhibitory peptides

Bioactive peptides are short peptides (3–20 amino acid residues in length) endowed of specific biological activities. The identification and characterization of bioactive peptides of food origin are crucial to better understand the physiological consequences of food, as well as to design novel foods, ingredients, supplements, and diets to counteract mild metabolic disorders. For this reason, the identification of bioactive peptides is also relevant from a pharmaceutical standpoint. Nevertheless, the systematic identification of bioactive sequences of food origin is still challenging and relies mainly on the so defined “bottom-up” approaches, which rarely results in the total identification of most active sequences. Conversely, “top-down” approaches aim at identifying bioactive sequences with certain features and may be more suitable for the precise identification of very potent bioactive peptides. In this context, this work presents a top-down, computer-assisted and hypothesis-driven identification of potent angiotensin I converting enzyme inhibitory tripeptides, as a proof of principle. A virtual library of 6840 tripeptides was screened in silico to identify potential highly potent inhibitory peptides. Then, computational results were confirmed experimentally and a very potent novel sequence, LMP was identified. LMP showed an IC50 of 15.8 and 6.8 µM in cell-free and cell-based assays, respectively. In addition, a bioinformatics approach was used to search potential food sources of LMP. Yolk proteins were identified as a possible relevant source to analyze in further experiments. Overall, the method presented may represent a powerful and versatile framework for a systematic, high-throughput and top-down identification of bioactive peptides.

Potential and practical implementation of bioactive polyvinylsuccinate derivatives

A review of the works reflecting the authors' research on the synthesis and study of the physico-chemical properties of polymer biologically active substances (BAS) is presented. The research results are analyzed in relation to the specific biological activity of polymers in order to establish the possibility of predicting their prolonged bioactive effect based on the values of the constants of formation, stability, dissociation and other characteristics of complexes of ionogenic polymers and BAS using methods of potentiometric titration, conductometry, viscometry, thermal analysis methods, equilibrium dialysis, biological tests.

Hybrid fusion protein as a dual protease inhibitor for the healing of chronic wounds.

Diseases bring about the need for interventions that pinpoint each specific aspect of the illness. Commonly, remission of a complex disease is accomplished by mixing treatments, medications, and therapeutics together in a fashion where they may negatively interact with each other or never arrive at the diseased site as a systemic heterogeneous mixture. Chronic wounds display intricacy as they are very localized and have their own environment where tissue deconstruction due to high levels of numerous proteases outweighs normal tissue reconstruction. This idea leads to the necessity of a protein that contains low diffusivity rates for localized treatment, strength against high concentrations of proteolytic species that lead to degradation of short chain peptides, while encompassing broad inhibitory effects against multiple proteases. Elastin-like peptides are an attractive, thermoresponsive, protein-based drug delivery partner as they contain low diffusivity and serve as a stable architecture for short chain peptide fusion. In this project, a novel elastin-like peptide-based protein has been created to target the inhibition of both human neutrophil elastase and matrix metalloprotease-2. As a biologic, this is unique as it is a protein with specific biological activities against multiple proteases, ultimately displaying the potential to mix and match differing biologically active peptides within one amino acid sequence.

Biomedical Effects of the Phytonutrients Turmeric, Garlic, Cinnamon, Graviola, and Oregano: A Comprehensive Review

Phytonutrients are plant foods that contain many natural bioactive compounds, called phytochemicals, which show specific biological activities. These phytonutrients and their phytochemicals may play an important role in health care maintaining normal organism functions (as preventives) and fighting against diseases (as therapeutics). Phytonutrients’ components are the primary metabolites (i.e., proteins, carbohydrates, and lipids) and phytochemicals or secondary metabolites (i.e., phenolics, alkaloids, organosulfides, and terpenes). For years, several phytonutrients and their phytochemicals have demonstrated specific pharmacological and therapeutic effects in human health such as anticancer, antioxidant, antiviral, anti-inflammatory, antibacterial, antifungal, and immune response. This review summarizes the effects of the most studied or the most popular phytonutrients (i.e., turmeric, garlic, cinnamon, graviola, and oregano) and any reported contraindications. This article also presents the calculated physicochemical properties of the main phytochemicals in the selected phytonutrients using Lipinski’s, Veber’s, and Ghose’s rules. Based on our revisions for this article, all these phytonutrients have consistently shown great potential as preventives and therapeutics on many diseases in vitro, in vivo, and clinical studies.

Clean and folded: Production of active, high quality recombinant human interferon-λ1

Type III interferons exhibit antiviral activity against influenza viruses, coronaviruses, rotaviruses, and others. In addition, this type of interferon theoretically has therapeutic advantages, in comparison with type I interferons, due to its ability to activate a narrower group of genes in a relatively small group of target cells. Hence, it can elicit more targeted antiviral or immunomodulatory responses. Obtaining biologically-active interferon lambda (hIFN-λ1) is fraught with difficulties at the stage of expression in soluble form or, in the case of expression in the form of inclusion bodies, at the stage of refolding. In this work, hIFN-λ1 was expressed in the form of inclusion bodies, and a simple, effective refolding method was developed. Efficient and scalable methods for chromatographic purification of recombinant hIFN-λ1 were also developed. High-yield, high-purity product was obtained through optimization of several processes including: recombinant protein expression; metal affinity chromatography; cation exchange chromatography; and an intermediate protein refolding stage. The obtained protein was shown to feature expected specific biological activity in line with published effects: induction of MxA gene expression in A549 cells and antiviral activity against influenza A virus.

Virtual Screening, Molecular Docking and Pharmacophore Modeling of Phytoconstituents of Flavones as Aldose Reductase Inhibitors

Flavones are an important class of naturally occurring heterocycles possessing various pharmacological activities. An in silico approach was carried out where 506 compounds containing flavone ring were utilised as ligand against the target aldose reductase enzyme. Aldose reductase is the rate-limiting enzyme in the polyol pathway, which indirectly causes diabetic complications like diabetic nephropathy and diabetic retinopathy. The flavone containing compounds retrieved from the PubChem were investigated by HTVS (high throughput virtual screening) followed by molecular docking using glide SP and XP docking module in Maestro of Schrodinger software. Among them, the best fifteen compounds were selected for further studies. The binding energy calculation was done using the Prime MM-GBSA module. PASS online prediction tools were used for predicting the antidiabetic activity of the compounds. Also, a pharmacophore model was generated for best interacted fifteen compounds by Phase, which can be used for evaluation of the characteristic features essential for this specific biological activity. The ADMET properties of the compounds were determined using the Qikprop module in the Schrodinger software.

Biodegradable Hypocrellin B nanoparticles coated with neutrophil membranes for hepatocellular carcinoma photodynamics therapy effectively via JUNB/ROS signaling

Hepatocellular carcinoma (HCC) is an inflammation-induced and chemotherapy-resistant common liver cancer, and a major cause of death. Some natural products have been found to be used as photosensitizers in photodynamic therapy of HCC. Due to its specific molecular structure diversities and biological activities, current status of HCC treatment with nature production remains unsatisfactory, owing largely to the toxicity, side effect and inefficiency to drug targeting. Herein, we show a nanoparticle-based broad-spectrum anti-inflammatory strategy that naïve neutrophil membrane-coated PLGA nanoparticles (NM-HB NPs) were constructed for synchronous nearinfrared fluorescence (NIR FL) imaging and photodynamic therapy (PDT) for HCC. Moreover, NM-HB NPs inhibited the expression of JUNB and promoted the ROS production. JUNB depletion enhanced the anti-HCC effect of NM-HB NPs. Importantly, it was shown that NM-HB NPs are well targeted to the tumor site and overcomes the blood circulation and immune elimination in vivo and vitro. In a mouse model of HCC, the neutrophil membrane-coated nanoparticles (NM-HB NPs) show significant therapeutic efficacy by PDT and suppressing tumor tissue increase. All results demonstrated that NM coated HB NPs representing a viable and effective treatment option for HCC.