Vegetation Species Research Articles

Purple Carrot Extract Exhibits a Neuroprotective Profile in th e Nigrostriatal Pathway in the Reserpine-induced Model of Parkinson 's Disease.

Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the nigrostriatal pathway. Even with scientific and technological advances, the therapeutic approaches used for the treatment of PD have shown to be largely ineffective in controlling the progression of symptoms in the long term. There is a growing demand for the development of novel therapeutic strategies for PD treatment. Different herbs and supplements have been considered as adjuvant to treat the symptoms of Parkinsonism. The carrot is one of the most consumed vegetable species worldwide, and its root is known for its content of anthocyanins, which possess antioxidant and antiinflammatory properties. This study evaluated the neuroprotective effect of purple carrot extract (CAR) in rats on the reserpine (RES)-induced progressive parkinsonism model. Male rats (6-month-old) received orally the CAR (400 mg/kg) or vehicle and subcutaneously RES (0.01 mg/kg) or vehicle for 28 days (Preventive Phase). From the 29th day, rats received CAR or vehicle daily and RES (0.1 mg/kg) or vehicle every other day (for 23 days, Protective phase). Behavioral tests were conducted throughout the treatment. Upon completion, the animals' brain were processed for tyrosine hydroxylase (TH) immunohistochemical assessment. Our results showed that the chronic treatment of CAR protected against motor disabilities, reducing the time of catalepsy behavior and decreasing the frequency of oral movements, possibly by preserving TH levels in the Ventral Tegmental Area (VTA) and SNpc. CAR extract is effective to attenuate motor symptoms in rats associated with increased TH+ levels in the Ventral Tegmental Area (VTA) and SNpc, indicating the potential nutraceutical benefits of CAR extract in a progressive parkinsonism model induced by RES.

Old lessons for new science: How sacred-tree metaphors can inform studies of the public-health benefits of the natural environment

Many studies have identified an association between exposure to the natural environment and improved public-health outcomes. However, much of this observational work lacks a theoretical foundation, so we look to the humanities for a stronger basis for green-health research, examining how trees have been used as religious metaphors and symbols for health and wellbeing. In particular, the tree of life, sacred trees, and other religious symbols provide a promising theoretical basis for green-health research. Based on this review, we propose the value of incorporating attributes such as vegetation species and size in exposure metrics, and considering the interactions between exposure attributes (e.g., species) and individual attributes (e.g., culture).

Cultivated plants and their wild relatives of Central Russia and the North Caucasus (results of a collecting mission of 2023)

In 2023, field explorations of cultivated plants and their wild relatives were carried out in the regions of the Volga Upland, the western part of the Caspian Lowland and the Stavropol Upland with the aim of further replenishing seed and herbarium collections of VIR.A total of 525 samples of CWR and cultivated plants were collected, including 200 are herbarium sheets, 323 seed samples of cultivated plants and CWR, and a couple of cuttings. The collected material encompasses 145 species, including 91 cultivars. Seeds of the cultivars were purchased at markets, in supermarket chains and from local residents. The following areas were examined in detail: the left bank of the Lower Volga (Peschany anabranch, Kigach anabranch, as well as the “Novaya Roshcha” protected area). In the Republic of Ingushetia the team collected samples of cherry plum, Caucasian pear, barberry, viburnum, Lonicera orientalis, sea buckthorn, raspberry, and Malus orientalis, which local residents use in their diet. Also, samples of spicy herbs such as savory, catnip, oregano, thyme, etc. were collected. Hops were sampled in the Tambov and Volgograd provinces, the Republics of Ingushetia, North Ossetia and Adygea, from abandoned household plots in Botanika village in Gulkevichi District (Krasnodar Territory). The exploration in vicinities of Derbent and of the wall of Naryn-Kala fortress in Derbent resulted in collecting a rare specimen of Secale sylvestre Host., which previously used to be a fairly common plant in the North Caucasus, but is very rare now. Vegetable crops were collected along the entire route of the collecting mission. It was not always possible to collect seeds, so a part of the material enriched the herbarium collection. The collected material reflects the use of a vast range of vegetable and spicy plant species by the local population.Collecting missions help to understand which species are of primary importance for the conservation of natural processes, biodiversity and the maintenance of local ecosystems. The genetic diversity of a region is a key object of research for geneticists and plant breeders. Landraces of vegetable, cereal and other crops may have unique properties, such as adaptation to local climate conditions or disease resistance. Work on the mobilization and conservation of the plant genetic diversity is the basis for modern breeding and genetic research.

Prediction of Adaptability of Typical Vegetation Species in Flood Storage Areas under Future Climate Change: A Case in Hongze Lake FDZ, China

China experiences frequent heavy rainfall and flooding events, which have particularly increased in recent years. As flood storage zones (FDZs) play an important role in reducing disaster losses, their ecological restoration has been receiving widespread attention. Hongze Lake is an important flood discharge area in the Huaihe River Basin of China. Previous studies have preliminarily analyzed the protection of vegetation zones in the FDZ of this lake, but the future growth trend of typical vegetation in the area has not been considered as a basis for the precise protection of vegetation diversity and introductory cultivation of suitable species in the area. Taking the FDZ of Hongze Lake as an example, this study investigated the change trend of the suitability of typical vegetation species in the Hongze Lake FDZ based on future climate change and the distribution pattern of the suitable areas. To this end, the distribution of potentially suitable habitats of 20 typical vegetation species in the 2040s was predicted under the SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5 climate scenarios using the latest Coupled Model Intercomparison Project CMIP6. The predicted distribution was compared with the current distribution of potentially suitable habitats. The results showed that the model integrating high-performance random forest, generalized linear model, boosted tree model, flexible discriminant analysis model, and generalized additive model had significantly higher TSS and AUC values than the individual models, and could effectively improve model accuracy. The high sensitivity of these 20 typical vegetation species to temperature and rainfall related factors reflects the climatic characteristics of the study area at the junction of subtropical monsoon climate and temperate monsoon climate. Under future climate scenarios, with reference to the current scenario of the 20 typical species, the suitability for Nelumbo nucifera Gaertn decreased, that for Iris pseudacorus L. increased in the western part of the study area but decreased in the eastern wetland and floodplain, and the suitability of the remaining 18 species increased. This study identified the trend of potential suitable habitat distribution and the shift in the suitability of various typical vegetation species in the floodplain of Hongze Lake. The findings are important for the future enhancement of vegetation habitat conservation and suitable planting in the study area, and have implications for the restoration and conservation of vegetation diversity in most typical floodplain areas.

Arbuscular mycorrhizal fungi affect early phenological stages of three secondary vegetation species in a temperate forest

The relationship between arbuscular mycorrhizal fungi (AMF) and secondary vegetation (SV) species at early phenological stages is critical for the successful establishment of these plants on disturbance sites in temperate forests. The main objective of this research is to evaluate the effect of AMF colonization on the early phenological stages (germination and early growth) of three shrub species present in the SV of a temperate forest in central Mexico. We collected soil from different sites in the Abies religiosa forest in central Mexico. We collected seeds of Acaena elongata, Ageratina glabrata, and Solanum pubigerum. We used a controlled experimental design with pasteurized soil (-AMF treatments) and unpasteurized soil (+ AMF treatments). We monitored germination percentage, growth (shoot and root weight and total biomass), AMF root colonization, and the mycorrhizal response index (MRI) for each plant species. All three species tested benefited by AMF, showing higher germination rates. Shoot and root weight and total biomass were significantly higher in the + AMF treatment. Solanum pubigerum showed greater stem length and Ageratina glabrata showed greater root development due to AMF. Ageratina glabrata and Acaena elongata were the most responsive to AMF as indicated by MRI. This research underscores the critical role of AMF in the early phenological stages of SV and highlights the potential ecological benefits of AMF in supporting plant germination and plant growth. These results suggest that AMF enhance germination and early growth of secondary vegetation species, which can be considered in management plans for forest ecosystems.

Physio-Anatomical Potentials of Some Vegetable Species for Atmospheric Humidification and Purification in Ojo Area, Lagos, Nigeria

Physio-Anatomical Potentials of Some Vegetable Species for Atmospheric Humidification and Purification in Ojo Area, Lagos, Nigeria

Quantifying vegetation cover on coastal active dunes using nationwide aerial image analysis

AbstractCoastal active dunes provide vital biodiversity, habitat, and ecosystem services, yet they are one of the most endangered and understudied ecosystems worldwide. Therefore, monitoring the status of these systems is essential, but field vegetation surveys are time‐consuming and expensive. Remotely sensed aerial imagery offers spatially continuous, low‐cost, high‐resolution coverage, allowing for vegetation mapping across larger areas than traditional field surveys. Taking Aotearoa New Zealand as a case study, we used a nationally representative sample of coastal active dunes to classify vegetation from red‐green‐blue (RGB) high‐resolution (0.075–0.75 m) aerial imagery with object‐based image analysis. The mean overall accuracy was 0.76 across 21 beaches for aggregated classes, and key cover classes, such as sand, sandbinders, and woody vegetation, were discerned. However, differentiation among woody vegetation species on semi‐stable and stable dunes posed a challenge. We developed a national cover typology from the classification, comprising seven vegetation types. Classification tree models showed that where human activity was higher, it was more important than geomorphic factors in influencing the relative percent cover of the different active dune cover classes. Our methods provide a quantitative approach to characterizing the cover classes on active dunes at a national scale, which are relevant for conservation management, including habitat mapping, determining species occupancy, indigenous dominance, and the representativeness of remaining active dunes.

Flora Current State of Rosaceae Woody Species in Mountain Xerophytic and Steppe Vegetation of Ordubad District

The current state of the tree family Rosaceae is considered. As a result of the research, the taxonomic composition, life forms, ecological groups, geographical areas and classes of tree species of the Rosaceae family, widespread in the mountain-xerophytic and steppe vegetation of the flora of the Ordubad district, were revealed. When characterizing the Rosaceae tree species of the mountain-xerophytic and steppe zone of the flora of the Ordubad region, it was established: Rosa— 14 (29.1%), Prunus — 6 (12.5%), Crataegus — 5 (10.4%), Pyrus — 5 (10.4%), Sorbus — 5 (10.4%), Cotoneaster — 5 (10.4%), Rubus— 3 (6.25%), Spiraea — 2 (4.16%) and 1 (2.08%) each of Malus, Louiseaniaand Pyracantha. The structure of the flora of the Ordubad region is dominated by species belonging to the genus RosaL. They are part of the shrub steppes and form independent groups and unique communities.

Miners' diet in the Naḥal ‘Amram copper mines (southern ‘Arabah Valley) during the Roman-Byzantine periods

This study investigates the diet of copper miners at Naḥal ‘Amram (southern Negev Desert), through the examination of the well-preserved remains of plants, mammal and fish bones, dating to the Roman-Byzantine periods (1st–5th centuries CE), recovered during excavations undertaken in three different localities at this mining complex.Our analyses revealed that the miners enjoyed a varied and nutritious diet, comprising nine species of fruits and vegetables, alongside meat from two domestic animal taxa and representatives of eight different families of fish. While the fish and animal foods were brought to the mines from the Red Sea and neighboring regions, the vegetal food originated from a variety of different sources, some nearby, others quite distant.We suggest that since copper was traded from Naḥal ‘Amram, the foods arrived at the mines via the same trade routes, from settlements along the ‘Arabah, and these foodstuffs were likely bartered in exchange for copper. The results imply that the people engaged in copper mining were likely a local desert population, Nabataeans, supplementing their food needs from external sources and so enjoying improved nutritional status.Beyond its findings, the importance of this study lies in the combination of several disciplines, a methodology which can be useful for future research and provide a more reliable understanding of reconstructing the ancient diet.

Salt marshes for nature-based flood defense: Sediment type, drainage, and vegetation drive the development of strong sediment beds

In face of sea-level rise and increasing risks for storm impacts on shorelines, there is a growing demand for developing nature-based flood defenses, for example by restoring or creating salt marshes in front of engineered structures such as dikes. However, salt marshes can only optimally provide flood defense if their sediment beds are erosion resistant, even under very high flow velocities. It remains unknown how fast sediment strength develops in marshes restored or created for nature-based flood defense. Therefore, this study investigated how 1) sediment type, 2) tidal drainage depth and duration, and 3) pioneer vegetation species drive the development rate of sediment strength. A controlled experiment was set up with pots filled with two sediment types, which were either left bare or planted with Spartina anglica or Scirpus maritimus, two dominant salt marsh pioneers in NW Europe. All treatments were subjected to four different tidal regimes with different tidal drainage depth and duration. The results showed that sandy mud (with a 37% silt and clay fraction) led to much stronger sediments than fine mud (with a 77% silt and clay fraction). Sediment strength was higher in the treatments with deeper tidal drainage depth and longer drainage duration. The presence of vegetation increased sediment strength and this effect was stronger with Scirpus maritimus than with Spartina anglica. Plant roots increased sediment strength directly, and the presence of vegetation also seemed to increase sediment strength through enhanced evaporation and transpiration. From these results it can be concluded that to restore or create erosion resistant salt marshes for flood defense, it is essential to ensure that marshes can form at relatively high elevations from well-draining sand-mud mixtures, thereby also ensuring vegetation growth.

Dietary Biodiversity and Diet Quality in Dutch Adults.

Dietary biodiversity, defined as the variety of consumed plants, animals and other organisms, can be measured by dietary species richness (DSR). This study investigated associations between DSR and diet quality in Dutch adults. Dietary intake data of 2078 Dutch participants, aged 19 to 79 years, were collected by the Dutch National Food Consumption Survey between 2012 and 2016 via two non-consecutive 24-h dietary recalls. DSR scores were calculated based on the total count of unique species consumed per individual over the two measurement days. An overall DSR score and separate scores for fruit and vegetable species consumption were calculated. The Dutch Healthy Diet index 2015 (DHD15-index) was used to measure diet quality. Linear regression analyses were performed to investigate associations between DSR scores and DHD15-index. Analyses were stratified by age and adjusted for relevant confounders. In total, 157 unique species were identified within the investigated food groups. On average, individuals consumed 13 unique species over two days (SD 4.55). For every additional species consumed, the DHD15-index increased by 1.40 points (95%CI 1.25-1.55). Associations between DSR and DHD15-index were higher in younger adults. DSR fruit showed the strongest associations with DHD15-index (β 4.01 [95%CI 3.65-4.38]). Higher DSR scores are associated with higher diet quality in Dutch adults. These newly developed DSR scores create opportunities for further research to explore the implications of dietary biodiversity in Western diets on health and related outcomes.

Topsoil volume optimization in the restoration of post‐mined areas

Transferring topsoil to severely degraded areas, such as mined areas, is an effective restoration technique. However, topsoil from pristine areas is a limited resource, making it important to seek techniques that optimize its use. Thus, this study assessed the effects of adding a small topsoil volume (10 L/m2 or 1 cm layer), a seed mix of 14 species, and litter (30 L/m2) alone and in different combinations in the restoration of a rupestrian grassland area degraded by bauxite mining. The substrate in the degraded area was turned prior to applying treatments. Vegetation cover, density of individuals, and species richness were measured 30 months after the experiment was set up. The isolated use of each material and the simultaneous addition of topsoil to the seed mix were not effective. The simultaneous use of topsoil, litter, and seed mix provided the best result, presenting the highest vegetation coverage (60%), density (67 ind/m2), and species richness (26 in 12 m2). Although, to a lesser extent, the combination of seed mix and litter also presented satisfactory results. The combination of the three materials promoted the revegetation of an area that had been without vegetation for around 50 years, presenting a density of individuals and species richness comparable to those obtained with the use of thick topsoil layers (15–30 cm). Furthermore, the topsoil volume used was optimized.

Human health risk assessment of heavy metals in vegetables of Bangladesh

This study aims to evaluate the heavy metal concentration in fifteen species of vegetables as well as associated health risk. Atomic absorption spectrometry is used to assess heavy metals. The mean concentrations of Pb, Cd, Cr, Ni and Fe in vegetables were 4.78, 0.713, 9.266, 0.083, 5.06 mg/kg/fw exceeding the reference value of FAO/WHO indicating unsafe to consumption. Based on principal component analysis, the Pb, Cr, Ni and Fe are from same sources. Health risk was estimated in terms of estimated daily intake (EDI), target hazard quotient, hazard index (HI) and cancer risk (CR). The EDI values of metals except Cr were found to be lower than maximum tolerable daily intake (MTDI). The total THQs of metals were > 1 indicating non-carcinogenic health risk. The individual HI values for vegetables except potato (0.831) and total HI values were found to be > 1 (94.747). The TCR of Pb, Cd and Cr were > 1.0E−04 which indicating carcinogenic risk. Fruit and pod vegetables contribute much in carcinogenic risk for Pb and Cr whereas fruit, root and stems vegetables for Cd. The study revealed potential human health risk associated with the consumption of different types of vegetables in Bangladeshi adult population that might assist the regulatory bodies to develop new strategies to minimize the risk to human.

Quantitative and Qualitative Production of Species Cucumis metuliferus and the Potential for Adaptation in the Context of Current Climate Change.

Cucumis metuliferus E. Meyer ex Naudin belongs to the family Cucurbitaceae. It is an annual vegetable species known as horned melon or kiwano. Fruits are recommended for the daily diet because they provide vitamins, minerals, and phytochemicals to alleviate malnutrition and improve human health. In this study, kiwano was cultivated in the climatic conditions of Southwestern Romania, which is characterized by warm summers, lower precipitation, and high temperatures, ensuring optimal conditions for growth and development. The fruits were harvested at three stages of consumption: immature stage (green), intermediate stage (white-yellow) or the mature stage (yellow-orange). They were also subjected to analysis on the productive yield and physical-chemical properties. The results showed that the number of fruits ranged from 13.9 to 15.3 fruits/plant, and their average weight had values from 204 g to 234 g, depending on the harvest stage. The results obtained for bioactive compounds and antioxidant activity indicated significant differences (p ≤ 0.05), depending on the harvesting stage. The highest values for the antioxidant capacity (140.90 μM TE/100g DPPH) and total polyphenols (48.78 TPC mg/100 g) were recorded in the immature fruit phase, as well as for the carbohydrate content (3.56%), total protein (1.98%), ascorbic acid (4.3 mg/100 g f.w.), and carotene (0.98 mg/100 g) in the mature fruit phase. These results may stimulate interest in the introduction of this species as a niche crop and for consumption as a source of natural antioxidants for the prevention of diseases due to oxidative stress.

Precipitation and time effects on vegetation change in Southern Arizona abandoned farmland

Farmland abandonment represents a significant issue globally, and in arid environments reclaiming ecosystems is challenging. In the Sonoran Desert, much irrigated farmland has been abandoned since 1950. Our objective was to compare changes in vegetative (canopy) cover and species over a 26-year period on abandoned farmland and unfarmed controls in southcentral Arizona (precipitation <300 mm/year). We evaluated time since abandonment and precipitation effects on these variables between 1976 and 2003 with point frame methods on eight farmland sites using stepwise multiple regression. Results suggest antecedent precipitation (AP) is more important than time since abandonment in explaining cover changes. On 26 abandoned sites, we used non-parametric ANOVA to show that vegetative cover (point frame and line intercept methods) declined by about 43% from 1977 to 2023, which was greater than on non-farmed controls. Few farmlands had cover and species composition like reference communities in 2003. Paired farmland-control sites exhibited similar vegetation in 1977 and 2003 suggesting local factors (e.g., soil chemistry, roadways) may have overwhelmed that of farming. Uninterrupted (natural) surface water flow may predict where revegetation was most successful. Our research indicates that farmland may exist in alternative states, and passive revegetation will not reestablish native Sonoran Desert communities on most abandoned farmland sites.

Interplay among manures, vegetable types, and tetracycline resistance genes in rhizosphere microbiome.

The rapid global emergence of antibiotic resistance genes (ARGs) is a substantial public health concern. Livestock manure serves as a key reservoir for tetracycline resistance genes (TRGs), serving as a means of their transmission to soil and vegetables upon utilization as a fertilizer, consequently posing a risk to human health. The dynamics and transfer of TRGs among microorganisms in vegetables and fauna are being investigated. However, the impact of different vegetable species on acquisition of TRGs from various manure sources remains unclear. This study investigated the rhizospheres of three vegetables (carrots, tomatoes, and cucumbers) grown with chicken, sheep, and pig manure to assess TRGs and bacterial community compositions via qPCR and high-throughput sequencing techniques. Our findings revealed that tomatoes exhibited the highest accumulation of TRGs, followed by cucumbers and carrots. Pig manure resulted in the highest TRG levels, compared to chicken and sheep manure, in that order. Bacterial community analyses revealed distinct effects of manure sources and the selective behavior of individual vegetable species in shaping bacterial communities, explaining 12.2% of TRG variation. Firmicutes had a positive correlation with most TRGs and the intl1 gene among the dominant phyla. Notably, both the types of vegetables and manures significantly influenced the abundance of the intl1 gene and soil properties, exhibiting strong correlations with TRGs and elucidating 30% and 17.7% of TRG variance, respectively. Our study delineated vegetables accumulating TRGs from manure-amended soils, resulting in significant risk to human health. Moreover, we elucidated the pivotal roles of bacterial communities, soil characteristics, and the intl1 gene in TRG fate and dissemination. These insights emphasize the need for integrated strategies to reduce selection pressure and disrupt TRG transmission routes, ultimately curbing the transmission of tetracycline resistance genes to vegetables.

Modeling wave attenuation through vegetation patches: The overlooked role of spatial heterogeneity

Coastal wetlands serve as vital buffers against extreme hazards due to the wave-dissipating capacity of vegetation. While most studies assume a spatial-uniform vegetation cover when modeling wave attenuation through wetlands, they are far from homogeneous in reality due to life-stage dependent growths, die-offs, and zonation of different vegetation species. In the present study, the XBeach model was applied to explore the role of vegetation spatial heterogeneity on wave attenuations. Random distributed vegetation maps with the same coverage but diverse patch sizes are generated to replicate the complex patterns of real wetland vegetations. We focus on the attenuation of solitary waves and wave groups, representing tsunami and storm waves, respectively. Different wave heights, vegetation densities, and land slopes are considered. For solitary wave cases, it is found that smaller patch sizes lead to higher wave attenuation rates, lower run-up, and less inundation extents. For wave group cases, it is found that the attenuation rates are dependent not only on patch sizes but also wave frequencies; while the higher frequency wave components are attenuated at similar magnitudes by different patch sizes, the infragravity waves are much less attenuated by larger patch sizes due to the penetrating effects through the major gaps between patches. Both cases revealed that under the same vegetation coverage, smaller patch sizes outperform their larger counterparts in terms of wave attenuation capacity. Out study highlights the importance of vegetation spatial heterogeneity, which are seldom considered in evaluating the wetlands' capacity of attenuating extreme waves.

Vegetation composition and organic carbon content along right-of-ways on Interstate Highway 35 in Bexar County, Texas

Roadside habitat through major urban areas may offer remnant examples of natural grasslands. These habitats may be subjected to frequent as mowing and exposure to automobile emissions and runoff. This study was conducted on interstate highway right-of-ways in San Antonio, Texas, U.S.A. We compared the vegetation species and diversity, and the organic carbon of leaf litter, plants, and soils. Two non-native grasses accounted for 50.9% cover, while all native forbs and grasses accounted for only 9.8% cover. The mean biomass of non-native grasses was 4.5 times greater than that of all native species. Organic carbon content of leaf litter, plants, and soils was variable among the study sites, possibly due to management practices and a drought that occurred during the study. The mean organic carbon content in the upper 20 cm soil was 5.0 kg·C/m2 and organic carbon content was greater in the upper 10 cm than organic carbon in the 10.1 to 20 cm portion. Cynodon dactylon and Bouteloua curtipendula exhibited the greatest photosynthesis efficiency indicating these grasses are more adaptable to hot summer temperatures found in Central Texas. The organic carbon content along a major interstate was 13793 kg·C/ha for leaf litter, vegetation, and the upper 20 cm of the soil. We conclude that interstate highways provide habitat for some native species, but the vegetation along right-of-ways is dominated by two non-native grasses. It appears unlikely that roadside habitat can be restored to resemble native grasslands without large scale and costly restoration efforts.

Land use changes effect by slash and burn cultivation to understory diversity composition and soil degradation

This study contributes to understanding ecological changes in land use following slash-and-burn land clearing. This study aimed to compare the richness and density of plant species and soil fertility in farmland cultivated for one year (1 Y), three years (3 Y), and five years (5 Y) after slash-and-burn, with the conditions in the secondary forest (SF) in Berau Regency of East Kalimantan. Understory plant taxon types, numbers, and soil organic matter were measured in each region using a series of nested plots. The size of each observation plot was 20 x 100 m. Data on understory vegetation composition was collected using 1 x 1 m mini plots at 20 sampling points and divided into five blocks, with a 10 m spacing between sampling points on each property. Understory vegetation species and cover percentage, litter cover percentage, open soil percentage, and soil fertility were measured for each observation plot. The results showed that the land cover percentage increased each year during the 5 years transition from secondary forest to farmland. Furthermore, within the 5 years, there was a change in understory species, particularly the presence of grasses and sedges at 3 and 5 years after the land use change. However, the transition from secondary forest to farmland within 5 years did not alter soil organic matter and total nitrogen.

Arbuscular Mycorrhizal Fungi Consortia in Six Vegetable Crops in the Tepeaca Valley, Puebla, Mexico

The objectives of this work were to search for and evaluate arbuscular mycorrhizal fungi (AMF) in six crops of agricultural importance in the horticultural region of Tepeaca valley, Puebla, Mexico, where Cambisols type soil predominates since the communities and consortia of AMF and their functionality in various crops of economic importance in Mexico are still unknown. The search for and identification of AMF spores was carried out in dif ferent vegetable crops. Five subsamples were collected from 6 dif ferent crop fields with vegetables (cilantro, cabbage, spinach, parsley, lettuce, and pumpkin); each subsample consisted of soil and roots. Roots 5 mm long were selected and stained using the Phillips-Hayman technique. Spore extractions and physicochemical determinations (soil texture, pH, relative humidity (RH), and electrical conductivity (EC)) were performed from soil. The results obtained from staining showed vesicles and spores in roots except in the pumpkin subsamples; of spore extractions from the soil of the six crops, 9 genera and 14 morphotypes of mycorrhizae were found, highlighting Funneliformis mosseae, Claroideoglomus Claroideum, and Glomus sp. as dominant species. By soil physicochemical characterizations were found: sandy texture, pH between 5.8 to 6.8; RH of 9.4 to 13%, and EC of 0.10 to 0.93 mS cm-1. In conclusion, the analyses of similarity and main components suggested that species of vegetables and soil conditions do not af fect the spore abundance, but they do af fect the richness and composition of AMF species, observing particular mycorrhizal consortia for each crop; therefore, it is suggested to evaluate their ef fectiveness in each of the crops to promote them as biofertilizer in the agricultural region of Tepeaca valley, Puebla, Mexico.