Lichen Species Research Articles

“Buthowtruethatis, Idonotknow”: the influence of written sources on the medicinal use of fungi across the western borderlands of the former Soviet Union

Fungi have been used for medicinal purposes for many centuries. This study, based on 35 historical written sources and 581 in-depth semi-structured interviews from eight countries in the western borderlands of the former Soviet Union, investigates the medicinal use of fungi by local communities. We compared the taxa and uses obtained from fieldwork and historical sources with works that advocated fungi use within Soviet herbals, representing the centralised medical system. During fieldwork, we identified eight locally used fungi and one lichen. The highest numbers of medicinal uses were documented in Russia, Estonia and Ukraine. Studies published before the Soviet era listed 21 fungal taxa and one lichen species used in the study region. However, only six of these taxa were mentioned as used by people in our field studies (Amanitamuscaria, Boletusedulis, Lycoperdon, Morchella, Phallusimpudicus and Cetrariaislandica). Notably, these same six taxa were consistently endorsed in Soviet herbals. Of the remaining three taxa recorded in the fieldwork, none were mentioned in historical written sources. However, they were promoted either in Soviet herbals (Inonotusobliquus, Kombucha) or later popular publications (Cantharelluscibarius). This highlights the significant influence of written sources on the use of fungi for medicinal purposes within the studied local communities.

De Novo Genome Assembly of Toniniopsis dissimilis (Ramalinaceae, Lecanoromycetes) from Long Reads Shows a Comparatively High Composition of Biosynthetic Genes Putatively Involved in Melanin Synthesis.

Lichens have developed numerous adaptations to optimize their survival in various environmental conditions, largely by producing secondary compounds by the fungal partner. They often have antibiotic properties and are involved in protection against intensive UV radiation, pathogens, and herbivores. To contribute to the knowledge of the arsenal of secondary compounds in a crustose lichen species, we sequenced and assembled the genome of Toniniopsis dissimilis, an indicator of old-growth forests, using Oxford Nanopore Technologies (ONT, Oxford, UK) long reads. Our analyses focused on biosynthetic gene clusters (BGCs) and specifically on Type I Polyketide (T1PKS) genes involved in the biosynthesis of polyketides. We used the comparative genomic approach to compare the genome of T. dissimilis with six other members of the family Ramalinaceae and twenty additional lichen genomes from the database. With only six T1PKS genes, a comparatively low number of biosynthetic genes are present in the T. dissimilis genome; from those, two-thirds are putatively involved in melanin biosynthesis. The comparative analyses showed at least three potential pathways of melanin biosynthesis in T. dissimilis, namely via the formation of 1,3,6,8-tetrahydroxynaphthalene, naphthopyrone, or YWA1 putative precursors, which highlights its importance in T. dissimilis. In addition, we report the occurrence of genes encoding ribosomally synthesized and posttranslationally modified peptides (RiPPs) in lichens, with their highest number in T. dissimilis compared to other Ramalinaceae genomes. So far, no function has been assigned to RiPP-like proteins in lichens, which leaves potential for future research on this topic.

Three New Species and a New Record of the Lichen Genus Peltula (Peltulaceae) from Helan Mountain in China.

In this study, a systematic taxonomic analysis was carried out on the lichen genus Peltula, collected from Helan Mountain in China; three new species (Peltula helanense, P. overlappine, and P. reticulata) and a new record (P. crispatula (Nyl.) Egea) for China were identified. Four species were identified by morph-anatomical, chemical, and phylogenetic analyses by combining two gene loci (ITS and LSU). Peltula helanense is with tiny individual thalli up to 1mm, attached by creamy-white cylindrical rhizoids and apothecia filling the whole squamule. Peltula overlappine is characterized by thallus squamulose forming rosette-shaped patches and squamules with distinctive thickened margins. Peltula reticulata is characterized by brownish brown thallus and squamules with densely reticulate upper surface. P. crispatula is characterized by irregular squamules attached to a tuft of hyphae. The four species are described in detail, compared, and discussed with similar species, and images of morpho-anatomical structures of the four species are also provided. Moreover, a key to the species of Peltula from Helan Mountain is provided. The results enrich the data of the genus Peltula and also indicate that the rich diversity of lichen species in Helan Mountain is worthy of in-depth study.

New data about overlooked terricolous lichen-forming and lichenicolous fungi of Ukraine

The new distribution data on rarely collected terricolous lichen species in Ukraine, Agonimia globulifera, Enchylium limosum, Epiphloea byssina, Gregorella humida, Sarcosagium campestre, Steinia geophana, Scytinium biatorinum, S. schraderi, and Verrucaria bryoctona, are provided. Notably, A. globulifera and S. biatorinum have been reported for the first time in the lowland part of Ukraine. Furthermore, the lichenicolous fungus Llimoniella terricola, growing on Epiphloea byssina, is reported for the first time in Eastern Europe. Descriptions, along with notes on similar species, habitat preferences, and general distribution, are provided for each species.

Assessment of Lichen Diversity in a Part of Madhyamaheshwar Valley of Garhwal Himalaya, Uttarakhand, India

A comprehensive survey was carried out to evaluate the distribution pattern and diversity of lichens in the Madhyamaheshwar valley, Rudraprayag district, Uttarakhand. According to the data gathered, there are 60 species total, divided into 21 families and 33 genera. The extensive diversity of lichen growth types in the region is demonstrated by the presence of 25 species of crustose lichen, followed by 19 foliose and 16 fruticose. The data gathered from the survey indicates that the Parmeliaceae family, with 8 genera and 12 species, is the most prevalent family in the area, followed by the Cladoniaceae family, which has 1 genera and 8 species. The enumeration of lichens from Madhyamaheshwar valley survey reveals that as the altitude increases the number of lichen species also increases. More taxa will undoubtedly be added to the lichen flora of this valley by conducting a thorough study of lichen species in the surrounding areas. Future biomonitoring and bioprospecting studies in the area will be supported by the baseline data provided by the current lichen count.

Antibacterial mechanism of the methanol extract of Thamnolia subuliformis (Ehrh.) W. Culb against Staphylococcus aureus.

Thamnolia subuliformis (Ehrh.) W. Culb is a species of lichen with edible and medicinal applications in China. Our previous studies demonstrated that the methanol extract of Thamnolia subuliformis (METS) exhibits broad antibacterial activity and stability against foodborne pathogens. This study aimed to investigate the antibacterial mechanism of METS against Staphylococcus aureus using nontargeted metabolomics, focusing on cell wall and membrane damage. The results revealed that the minimum inhibitory concentration (MIC) was 0.625mg ml-1 and that METS had good biosafety at this concentration. METS caused significant damage to the cell wall and membrane integrity, based on both morphological observation by electron microscopy and the leakage of alkaline phosphatase, protein, and nucleic acid in the cell cultures. Treatment with METS at the MIC disrupted the lipid metabolism of S. aureus, causing a decrease in the metabolism of various phospholipids and sphingolipids in the cell membrane and an increase in the ratio of saturated fatty acids to unsaturated fatty acids. Moreover, it influenced intracellular amino acid and energy metabolism. These results shed light on the antibacterial mechanism of METS against S. aureus while also serving as a reference for the further development of natural antibacterial compounds derived from Thamnolia subuliformis.

Bioecological characteristics of lichenoflora of the Prisursky State Nature Reserve (Chuvash Republic)

The article presents a taxonomic and ecological-biomorphological analysis of the lichenoflora of the Alatyrsky site of the Prisursky State Nature Reserve. The State Nature Reserve consists of 3 clusters (Alatyrsky, Batyrevsky, Yalchiksky). The Alatyrsky section of the reserve is located in the central part of the Prisursky forest area and is represented by various types of forest. As a result of the analysis of the literature and our own field research, 145 species of lichens have been identified in a specially protected natural area of federal significance. Taxonomic analysis showed that the identified lichen species belong to 4 classes (Arthoniomycetes, Coniocybomycetes, Dothideomycetes, Lecanoromycetes), 14 orders (Arthoniales, Acarosporales, Caliciales, Candelariales, Coniocybales, Lecanorales, Lecideales, Peltigerales, Pertusariales, Pleosporales, Strigulales, Teloschistales, Trapeliales, Umbilicariales), 32 families and 68 genera. The predominant taxonomic diversity is the class Lecanoromycetes, which includes 131 species from 24 families, 10 orders. The class Arthoniomycetes is represented by one order (Arthoniales), 2 families and 6 species. The Coniocybomycetes class includes 2 orders – Coniocybales, Strigulales, 2 families and 4 species. The class Dothideomycetes consists of 1 order Pleosporales, 4 families and 4 species. Ecological and biomorphological analysis revealed that the largest number of lichens of the Prisursky reserve is represented by a monotonously typical group of life forms – 60 species, a dissected-lobed rhizoidal group of life forms is 25 species, an awl- or scyphoid group is 21 species.

Can lichens be indicators for air pollution monitoring in Kandy City, Sri Lanka?

The quality of the surrounding environment significantly impacts lichen diversity and composition, but crustose lichens, particularly those in disturbed environments, can persist. Although the effects of habitat disturbances on the morphology, anatomy, chemical composition, and fertility of lichen species have not been extensively researched, understanding their mechanisms is crucial for understanding their persistence in their environment. The study aimed to identify common lichen species in polluted and semi-polluted environments and compare their morphology, anatomy, chemical composition, and fertility. A two-kilometer stretch along the road that passes through the University of Peradeniya was selected as the semi-disturbed site based on previous records, while the area around Kandy Lake was designated as the polluted site. Using conventional keys, lichen species were identified, and using a hand lens, dissecting microscope, and compound microscope, the morphological traits of the thallus, apothecia, soredia, and isidia, as well as the anatomical features of the thallus, apothecia, and ascospores, were compared between the two sites. Thin-layer chromatography was used to separate and elucidate the secondary metabolites of lichen. Thirty-nine species were discovered on the university site, 15 in the area around Kandy Lake, and species gathered from both locations shared commonalities, including Physcia sp., Parmotrema sp., Lecanora sp., Graphis sp., and Lepraria sp. The color difference between all species, as measured by mean dE, is visible to the naked eye and changes from a pale to a dark hue from a semi-disturbed site to a polluted one. In comparison to disturbed sites, the majority of species displayed an increased mean area in semi-polluted sites. Layer thickness increases or decreases depending on how well each layer can withstand disruptions. Depsinose has been recognized as a potential class of metabolites generated by lichens present in both locations. Only lake sites exhibit toxic metal compounds, as opposed to semi-disturbed sites. Every pH value was acidic, and most species at the lake site had more acidic pH than those at the university site. There is no specific trend in apothecial number and ascospore numbers in common lichen species in both sites. The current study showed that changes in certain morphological and anatomical biochemical characteristics can be triggered by the environment, suggesting that the quality of the environment significantly affects the growth and reproductive success of lichens, thereby survival or extinction, and proposed that bioindicator and bioaccumulator species concerning the changes occurred.

Continental scale comparison of mycobiomes in Parmelia and Peltigera lichens from Turkey and South Korea

BackgroundLichens, traditionally considered as a simple partnership primarily between mycobiont and photobiont, are, in reality, complex holobionts comprised of a multitude of microorganisms. Lichen mycobiome represents fungal community residing within lichen thalli. While it is acknowledged that factors like the host lichen species and environmental conditions influence the structure of the lichen mycobiome, the existing research remains insufficient. To investigate which factor, host genus or location, has a greater impact on the lichen mycobiome, we conducted a comparative analysis of mycobiomes within Parmelia and Peltigera collected from both Turkey and South Korea, using high-throughput sequencing based on internal transcribed spacer region amplification.ResultsOverall, the lichen mycobiome was dominated by Capnodiales (Dothideomycetes), regardless of host or location. At the order level, the taxonomic composition was not significantly different according to lichen genus host or geographical distance. Hierarchical clustering of the top 100 abundant ASVs did not clearly indicate whether the lichen mycobiome was more influenced by host genus or location. Analyses of community similarity and partitioning variables revealed that the structure of the lichen mycobiome is more significantly influenced by location than by host genus. When analyzing the core mycobiome by host genus, the Peltigera mycobiome contained more ASV members than the Parmelia mycobiome. These two core mycobiomes also share common fungal strains, including basidiomycete yeast. Additionally, we used chi-squared tests to identify host genus-specialists and location-specialists.ConclusionsBy comparing lichen mycobiomes of the same genera across different countries, our study advances our comprehension of these microbial communities. Our study elucidates that, although host species play a contributory role, geographic distance exerts a more pronounced impact on the structure of lichen mycobiome. We have made foundational contributions to understanding the lichen mycobiome occupying ecologically crucial niches. We anticipate that broader global-scale investigations into the fungal community structures will provide more detailed insights into fungal residents within lichens.

Antimicrobial activity of fungal extracts identified from thirty-two lichen species

The isolation of fungi from lichens, the study of their properties and the determination of their biological activity make an actual contribution to the production of a new antibiotic by the pharmaceutical industry. During the research, 22 lichen samples were collected. When determining the type of lichens, the characteristics of their growth were taken into account and the morphological identification was made according to the description of the monograph by Wang (2012). 19 fungal isolates were isolated from the core apothecia of lichen samples. Purified isolates were identified by detecting fungal DNA using PCR. These isolates were saprophytes, phytopathogens, pathogens of endolichaine and symbiotic fungi. The antimicrobial properties of 19 species of identified fungi were determined against strains of Escherichia coli 25922 B-RKM 0447, Staphylococcus aureus 6538 — RKM 0470, Serratia marcescens В-RKM 0832 and antifungal activity against Candidae albicans 885–653, 8 active types were identified: Chaetomum globosum, Trichoderma atroviride, Fungal sp, Coniochaeta ligniaria, Trichoderma harzianum, Hypocrea lixii, Daldinia loculata, Trichoderma viride, Neurospora tetraspora. The obtained extracts against the strains S. marcescens, E. coli, St. aureus, C. albicans showed an average sensitivity of 15±0.2 – 23±0.5.

Do gypsophile lichens exist? Analysis of the affinity for the gypsum substrate of lichen species from Spain

Gypsum soils, despite physico-chemical constraints, harbor a unique biota composed of specialist (gypsophiles) and stress-tolerant non-specialist species (gypsovags). Gypsophily has been addressed in plants, although is important to ask whether lichen communities also contain gypsophile species. Therefore, our main aim is the analysis of the affinity of lichens for the gypsum substrate in Spain. Affinity was estimated using two methods: a “geological method”, overlapping lichen occurrence data on a geological map of Spain; and a “biological method”, overlapping the occurrences on a map constructed with the distribution of plant gypsophiles. To assess the accuracy of both methods, we compared them with a literature review. Lichen occurrence data was obtained from GBIF. The biological method was the most accurate as it showed similar percentages to the literature review. The affinity for gypsum substrate has been effectively demonstrated by the employment of these methods, probing the existence of a group of lichens considered gypsophiles. Twenty lichen species are considered gypsophiles, 7 strict and 13 preferential (ca. 40% of 50 taxa analyzed), and 30 gypsovags. This approximation can apply to the study of the affinity for the substrate for other organisms/substrates, and for characterizing geological units when detailed geological maps are not available.

Ways of identifying lichen and plant species by the Nenets reindeer herders in Yamal

Yamal Nenets herders have historically developed a rich knowledge of lichens and vascular plants, which feature in the diet of their migratory reindeer herds in the tundra zone of northwest Siberia. In the Nenets language there are native names for certain species of lichens and other reindeer forage plants, including graminoids, herbs, shrubs, berries, and mushrooms. During participant fieldwork together with nomadic tundra Nenets herders, we documented names and definitions of reindeer food on herding territories during their long migration routes from the northern forest-tundra transition zone to the northern coastal tundra. Like many other Indigenous peoples of Siberia, Nenets have noticed that the Arctic is changing and some of its recent dynamics are seriously affecting their livelihood. The degradation of some lichen composition and cover on tundra pastures has also contributed to a decrease of herders’ linguistic palette for describing these losses in a concrete manner. Since the Nenets language is on the list of endangered languages of the world, this has an especially negative impact on the language skills and traditional knowledge of the younger generations of Nenets people, who may not know what these lichens look like and why they are important for the Nenets reindeer herding culture.

In-vitro Study on Anthelmintic and Insecticidal Activity of Macrolichens Collected from South Karnataka

In the present study, lichen samples were collected from the southern Karnataka state and were used for in-vitro anthelmintic and insecticidal activity. The three lichen samples Parmotrema tinctorum (Nyl.) Hale, Roccella montagnei Bèl. Emend. Awas, Everniastrum cirrhatum (Fr.) Hale was subjected to the extraction with solvents. The prepared extracts from the lichen species were evaluated for anthelmintic activity against Pheretima posthuma. 100% mortality was obtained from all the samples and it was compared with the reference drug albendazole for positive control. P. tinctorum methanolic extract showed the fastest result but on the other side, the slowest result was observed in the hexane extract. The insecticidal activity of the crude extracts of the lichens was tested against two insects Macrosiphum rosae (L.) and Sitophilus oryzae (L.). There was a 100% mortality rate observed in R. montagnei and E. cirrhatum methanolic extracts against M. rosae. In S. oryzae the methanolic extracts of P. tinctorum and E. cirrhatum with 75 and 50% mortality rates at 42 and 48 hours was observed. The promising results of lichen extracts towards insecticidal and anthelmintic activity were found to be a potential candidate for drug discovery and development against biopesticides.

Ecological Impacts of Coastal Protection on the Vegetation of Sandy Coasts at the German Baltic Sea Coast

Sand nourishments and groynes as coastal protection measures (CPM) address similar challenges on sandy coasts but take different approaches: while groynes are intended to reduce alongshore sediment transport and erosion, nourishments add new sediment to the system to compensate for erosion. The aim of this study is to compare the ecological effects of such measures on the vegetation. To this end, nutrient analysis and botanical mappings were carried out on a site with installed groynes, a site where sand nourishments are regularly carried out, and a control site without any CPM. In addition to an increase in nutrient availability after the sand nourishment, significant changes in plant species diversity and composition were also measured. The number of higher plants, mosses, and lichen species was lower at the nourishment site. The opposite impacts were observed at the groyne site: an increase in sediment cover by higher plants and mosses and a distinct increase in lichen species. The results suggest that groynes lead to a stabilization of the coastal system and enable dense vegetation growth. In contrast, sand nourishments lead to nutrient input and unstable habitat conditions, attracting certain plant communities but preventing the establishment of ground-covering vegetation.

Cliff nesting birds provide nutrient inputs to cliff ecosystems

Allochthonous inputs often link nutrient cycles between different ecosystems as nitrogen (N) that originates in one ecosystem is transferred to another. Mobile animals such as birds and fish can translocate nutrients between locations. Consequently, animal-mediated nutrient subsidies can shape plant communities, especially in nutrient-poor ecosystems. Terrestrial cliffs are typically nutrient-poor, receiving N from atmospheric deposition and cliff-top runoff. Cliff nesting birds deposit N-rich excrement onto the cliff during nesting, yet this potential nutrient input has rarely been demonstrated. Here we document an allochthonous input of N from cliff-nesting birds to terrestrial cliff vegetation. We sampled mineral N and vegetation on eight cliff systems in western North Carolina (USA) with nests from peregrine falcon (F. peregrinus; two sites) and common raven (Corvus corax; five sites). Two sites had nests but species identity was not confirmed. Cliff faces below bird nests had higher levels of ammonium (NH4+) than did faces without nests. Both ammonium and nitrate (NO3−) explained variability in cliff vegetation community composition, but site-level characteristics explained more of the variation than did N inputs alone. Lichens, the dominant taxonomic group, contributed the most to this variation and had higher species richness and diversity below nests. Indicator species analysis identified two nitrophilic lichen species below nests: Flavoplaca citrina and Physcia caesia. A third nitrophilic lichen, Polycauliona candelaria (syn. Xanthoria candelaria) was also found under nests. Bird excrement is likely a significant subsidy to nutrient-poor terrestrial cliff ecosystems, thus linking cliffs to more productive ecosystems and highlighting the need to include birds in conservation and management of cliffs.

Biomonitoring of airborne microplastics and microrubbers in Shiraz, Iran, using lichens and moss.

Microplastics (MPs) and microrubbers (MRs) determined in lichens and mosses around Shiraz. In lichens, MPs mainly thin fibres up to 1 MP g-1; MRs were < 0.1 MP g-1. In mosses, abundances were similar but with a greater fraction of larger, non-fibrous particles. Larger MPs and MRs decreased in abundance with distance and elevation from Shiraz. Around Shiraz, the common moss, Grimmia critina, would be the most suitable biomonitor. Lichens and mosses have been employed as biomonitors of atmospheric particulate pollutants, like metals and industrial solids, for many decades. Here, we evaluated the potential of nine species of crustose and foliose lichens and a widely distributed moss (Grimmia critina) to act as biomonitors of airborne microplastics (MPs) and microrubbers (MRs). About 200 lichens and 40 mosses were sampled across different altitudinal transects in the vicinity of Shiraz City, southwest Iran, and MPs and MRs were quantified and characterised after sample peroxidation. In most species of lichen, MP and MR abundance overall was < 1g-1 and < 0.1g-1, respectively, and the majority of plastics were fibres of < 10 µm in diameter and < 1000 µm in length. Respective weight normalised abundances of MPs and MRs were similar in G. critina, but there were greater proportions of both larger (> 1000µm) and non-fibrous particles among the MPs. In both lichens and moss, there was a greater number of larger MPs and MRs at locations closest to and at the same elevation as Shiraz than at more distant and elevated locations, suggesting an inverse relationship between particle size and distance travelled. Among the lichens, members of the genus Acarospora, with their areolated form, appeared to act as the most suitable biomonitors for MPs and MRs. Overall, however, the wide distribution of the moss, G. crinita, and its ability to intercept and accumulate a broader range of sizes and shapes of MPs and MRs make this species a better choice, at least in the type of environment studied.

COMPLEX RESEARCH EXPEDITION “CRILLON 2023”: FIRST FINDINGS AND PRELIMINARY RESULTS

In August 2023, a comprehensive expedition “Crillon 2023” was performed, covering both terrestrial and marine biotopes in the southeastern part of the Crillon Peninsula (Sakhalin Island, Russia). A group of specialists carried out field work in ichthyology, invertebrate zoology, entomology, botany, lichenology, bryology, mycology, parasitology, microbiology and marine biology. In the scarcely studied territory of the southeastern part of the peninsula, a notable amount of data on species diversity was collected, including more than 200 species of plants, 101 species of lichens, 74 species of mosses, 117 species of basidial macromycetes were recorded. Marine coastal communities of the littoral and sublittoral zones were studied, and diving methods were exploited. As many as 119 species of invertebrates and 20 species of seaweed have been recorded. Information was collected on 20 species of fish, including data on helminth infestation. Parasitological studies included the search for microsporidia in all available animal hosts, as well as the search for root crayfish - parasites of arthropods. Among the animals and plants found, there are rare and endangered species included in the regional and federal Red Books, as well as those previously not recorded for Sakhalin. The preliminary results obtained indicate great potential for further study of the eastern part of the peninsula from the standpoint of biological sciences, as well as the prospects for establishment a biological station in this area for long-term research and development.

Time-lag effects of habitat loss, but not fragmentation, on deadwood-dwelling lichens

ContextLandscape habitat amount is known to increase biodiversity, while the effects of habitat fragmentation are still debated. It has been suggested that negative fragmentation effects may occur with a time lag, which could explain inconsistent results. However, there is so far no empirical support for this idea.ObjectivesWe evaluated whether habitat amount and fragmentation at the landscape scale affect the species density of deadwood-dwelling lichens, and whether these effects occur with a time lag.MethodsWe surveyed deadwood-dwelling lichens in woodland key habitats in two regions in northern Sweden, and modelled their species density as a function of past (1960s) and present (2010s) habitat amount (old forest area) and fragmentation (edge density) in the surrounding landscapes.ResultsPresent habitat amount generally had weak positive effects on lichen species density. Positive effects of the past habitat amount were stronger, indicating a time lag in habitat amount effects. Habitat fragmentation effects were generally weak and similar whether fragmentation was measured in the past or the present landscapes, indicating no time lag in fragmentation effects.ConclusionsWe found a time lag effect of habitat amount, but not fragmentation. This result is not consistent with suggestions that time lags explain the mixed observations of fragmentation effects. Time-lag effects of habitat amount suggest that the studied lichen communities face an extinction debt. Conservation should therefore prioritize increasing the amount of old forest, for example by creating forest reserves, to maintain the current lichen diversity. More generally, our results imply that studies examining only the present habitat amount risk under-estimating its importance.

A New Rare Halogenated Depside from Lichen and Study of its Anti-Proliferative Activity.

Lichens are a symbiotic association of algae and fungus, belonging to the family Parmeliaceae. Some lichen species are edible and used as an active ingredient for preparation of exotic spices as well as folklore medicine to cure different kinds of ailments. A specimen of lichen was collected from Munner in the Kerala State of South India for chemical profiling. Chemical analyses of the diethyl ether extract of the defatted lichen led to the isolation of six phenols 1-6 with variation of relative abundance. Amongst them, the relative abundance of compound 3 was the greatest (1 % of crude extract) and it was identified as atranorin. The structures of known compounds were confirmed by comparison of their 1H-NMR, 13C NMR, and mass data with published values available in the literature. In vitro bioassay for anti-proliferative activity of these compounds has been conducted against various human cancer cell lines in comparison with paclitaxel as control using SRB assay. Interestingly, a new compound 5 was found along with previously reported compounds from this lichen. This new compound was designated as fluoroatranorin 5 which was reported for the first time herein. The structural characterization of a new depside was determined by spectral methods such as 1H-NMR, 13C NMR, 19F NMR, IR, LC-HRESI-MS, and LC-MS/MS study. Its structure was confirmed by single crystal X-ray diffraction study. This new compound was designated as fluoroatranorin 5 which was reported first time herein. Anti-proliferative activity of all these compounds was evaluated against six different cancer cell lines. The inhibitory activity, IC50 value of compounds 1-3 and 5 exhibited at 99.64, 102.04, 109.20, 53.0 and 2.4 μM on cancer cell lines HT-29 (colon), Hela (cervical), HT-29, HPAC (pancreas) and A2780 (ovarian cancer cell line) respectively in comparison with paclitaxel as control. The new compound 5 exhibited significant activity with IC50 value 2.4 μM on A2780 ovarian cancer cell line.

Lichens on &lt;i&gt;Agapetes&lt;/i&gt; D. Don Ex G Don (Ericaceae) in India

The paper deals with the lichens growing on Agapetes D. Don ex G. Don. There are 12 species of lichens belonging to 7 genera under 2 families found to grow on twigs of 13 species of Agapetes.