Galliform Species Research Articles

Reintroduction as a conservation tool for threatened Galliformes: the Red-billed Curassow Crax blumenbachii case study from Rio de Janeiro state, Brazil

Seventy-three of the 280 species of Galliformes are at risk of extinction, and all of them are threatened by human factors (mainly hunting and deforestation). Reintroduction is increasingly proposed as a conservation measure, although there is limited post-release monitoring to determine success. The science of reintroduction biology is still in its infancy in Brazil, mainly because most of the projects involving release of animals seldom include post-release monitoring, data collected are not published, and release areas are frequently chosen without any criteria. The Red-billed Curassow Crax blumenbachii reintroduction program in Brazil can be considered a good model for developing future Brazilian reintroduction projects. Systematic radio-tracking of released Red-billed Curassows has allowed detailed information on the ecology and behavior of the species. The information obtained through post-release monitoring will guide future management of the species, and it is a good example of the use of reintroduction as a conservation tool for threatened Galliformes.

Sequencing of the core MHC region of black grouse (Tetrao tetrix) and comparative genomics of the galliform MHC.

BackgroundThe MHC, which is regarded as the most polymorphic region in the genomes of jawed vertebrates, plays a central role in the immune system by encoding various proteins involved in the immune response. The chicken MHC-B genomic region has a highly streamlined gene content compared to mammalian MHCs. Its core region includes genes encoding Class I and Class IIB molecules but is only ~92Kb in length. Sequences of other galliform MHCs show varying degrees of similarity as that of chicken. The black grouse (Tetrao tetrix) is a wild galliform bird species which is an important model in conservation genetics and ecology. We sequenced the black grouse core MHC-B region and combined this with available data from related species (chicken, turkey, gold pheasant and quail) to perform a comparative genomics study of the galliform MHC. This kind of analysis has previously been severely hampered by the lack of genomic information on avian MHC regions, and the galliformes is still the only bird lineage where such a comparison is possible.ResultsIn this study, we present the complete genomic sequence of the MHC-B locus of black grouse, which is 88,390 bp long and contains 19 genes. It shows the same simplicity as, and almost perfect synteny with, the corresponding genomic region of chicken. We also use 454-transcriptome sequencing to verify expression in 17 of the black grouse MHC-B genes. Multiple sequence inversions of the TAPBP gene and TAP1-TAP2 gene block identify the recombination breakpoints near the BF and BLB genes. Some of the genes in the galliform MHC-B region also seem to have been affected by selective forces, as inferred from deviating phylogenetic signals and elevated rates of non-synonymous nucleotide substitutions.ConclusionsWe conclude that there is large synteny between the MHC-B region of the black grouse and that of other galliform birds, but that some duplications and rearrangements have occurred within this lineage. The MHC-B sequence reported here will provide a valuable resource for future studies on the evolution of the avian MHC genes and on links between immunogenetics and ecology of black grouse.

Low frequency specialization in the inner ear of the Ruffed Grouse (Bonasa umbellus).

Event Abstract Back to Event Low frequency specialization in the inner ear of the Ruffed Grouse (Bonasa umbellus). Jeremy R. Corfield1* and Andrew N. Iwaniuk1 1 The University of Lethbridge, Neuroscience, Canada Male ruffed grouse use their wings to produce a unique non-vocal auditory signal to attract females and defend a territory. This ‘drumming’ behavior consists of a male perching on a log and beating its wings rapidly to produce a low frequency (< 100Hz) sound. This is the primary auditory signal produced by the ruffed grouse and unlike all other grouse and related galliform species, the ruffed grouse has an extremely small vocal repertoire and do not incorporate vocalizations into their courtship display. Hearing lower frequencies likely requires specialization to the peripheral auditory system and we therefore predicted that ruffed grouse would differ in this respect from other galliforms. Because there is a well established correlation between morphology and physiology in the avian inner ear, we examined the auditory nerve, basilar papilla and hair cells in the ruffed grouse to identify specializations that could be associated with low frequency hearing. The ruffed grouse papilla was approximately 4.6 mm long and showed the typically avian, moderate curvature and tapered width. The total hair-cell count was about 9,200, which is similar to that of the comparably sized pigeon. The mechanosensitive stereovilli bundles of the hair cells showed the typical change from tall bundles with few stereovilli to short bundles with many stereovilli along the apical-to-basal tonotopic axis. In contrast to most birds, however, within the apical, low frequency part of the basilar papilla the stereovilli height remained reasonably consistent. The number of afferent fibers in the ruffed grouse auditory nerve was lower than most other birds that have been examined, with only around 6,200 fibers. An estimate of the average innervation density showed that the afferent innervation ratio in the ruffed grouse was 0.74, suggesting that for every hair cell there are 0.74 nerve fibers. This ratio is at the lower end of birds and is similar to that of the emu and pigeon, both of which have low frequency vocalizations. On the ruffed grouse papilla, hair cell shape changed gradually from a tall and thin type (THC) to a shorter and wider type (SHC), both from apex to base and, at the same longitudinal position, from neural to abneural, which is typical of other birds. However, the ruffed grouse had few hair cells that meet the conventional criterion for short hair cells. Overall, the inner ear of the ruffed grouse has some features that are typical of most birds and other features that it shares with the emu and pigeon, species known to have low frequency specializations to their inner ear associated with the low frequency vocalizations. Keywords: Basilar papilla, Hair cell, Inner ear, Ruffed grouse Conference: Tenth International Congress of Neuroethology, College Park. Maryland USA, United States, 5 Aug - 10 Aug, 2012. Presentation Type: Poster (but consider for Participant Symposium) Topic: Sensory: Audition Citation: Corfield JR and Iwaniuk AN (2012). Low frequency specialization in the inner ear of the Ruffed Grouse (Bonasa umbellus).. Conference Abstract: Tenth International Congress of Neuroethology. doi: 10.3389/conf.fnbeh.2012.27.00189 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Apr 2012; Published Online: 07 Jul 2012. * Correspondence: Dr. Jeremy R Corfield, The University of Lethbridge, Neuroscience, Lethbridge, Alberta, T1K 3M4, Canada, jr.corfield@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jeremy R Corfield Andrew N Iwaniuk Google Jeremy R Corfield Andrew N Iwaniuk Google Scholar Jeremy R Corfield Andrew N Iwaniuk PubMed Jeremy R Corfield Andrew N Iwaniuk Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Intronic deletions that disrupt mRNA splicing of the tva receptor gene result in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroup A.

The group of closely related avian sarcoma and leukosis viruses (ASLVs) evolved from a common ancestor into multiple subgroups, A to J, with differential host range among galliform species and chicken lines. These subgroups differ in variable parts of their envelope glycoproteins, the major determinants of virus interaction with specific receptor molecules. Three genetic loci, tva, tvb, and tvc, code for single membrane-spanning receptors from diverse protein families that confer susceptibility to the ASLV subgroups. The host range expansion of the ancestral virus might have been driven by gradual evolution of resistance in host cells, and the resistance alleles in all three receptor loci have been identified. Here, we characterized two alleles of the tva receptor gene with similar intronic deletions comprising the deduced branch-point signal within the first intron and leading to inefficient splicing of tva mRNA. As a result, we observed decreased susceptibility to subgroup A ASLV in vitro and in vivo. These alleles were independently found in a close-bred line of domestic chicken and Indian red jungle fowl (Gallus gallus murghi), suggesting that their prevalence might be much wider in outbred chicken breeds. We identified defective splicing to be a mechanism of resistance to ASLV and conclude that such a type of mutation could play an important role in virus-host coevolution.

Testing radiotransmitter attachment techniques on northern bobwhite and chukar chicks

AbstractWe compared 4 external radiotransmitter attachment techniques to determine the optimum attachment method on chicks of 2 galliform species. The attachment methods included tissue glue, silicone gel, suturing, and leg harness. The study was conducted in captivity with a 2‐phase assessment: first with northern bobwhite (Colinus virginianus), and then chukar (Alectoris chukar) chicks. We applied each technique and assessed effects on growth rates, retention times, ease of attaching the transmitter, and effects on physical development. No apparent adverse impacts on chicks were observed for any of the attachment techniques. We found the leg‐harness technique was most reliable in terms of retention time, required the least amount of handling time, and was the simplest to administer. Modifications to our suture technique likely would result in similar retention times, but would still require additional handling time and complexity in attaching transmitters. © 2011 The Wildlife Society.

Genetic evidence for male-biased dispersal in Elliot’s Pheasant (Syrmaticus ellioti) in China

Sex-biased dispersal, in which individuals of one sex tend to disperse and breed at a greater distance from their natal site than individuals of the opposite sex, appears to be common in vertebrate organisms and is very important to population structures and dynamics. Many studies have documented the dispersal patterns of monogamous birds; however, observations and data are few for polygynous birds. In our study, we report on the indication of sex-biased dispersal in Elliot's Pheasant (Syrmaticus ellioti), a vulnerable species endemic to China, using polymorphic DNA microsatellite loci (105 individual birds and seven loci) and mitochondrial DNA control-region sequences (63 birds). Contrary to the traditional con- cept that males are the more philopatric sex and females the more dispersing sex in birds, all the genetic information extracted from the two markers suggests that male-biased dispersal is predominant in Elliot's Pheasant. We argue that polygynous species in Galliformes without lekking behavior are more likely to ex- hibit male-biased dispersal patterns, consistent with the expected results based on the polygynous mating system of Elliot's Pheasant.

Genotyping of black grouse MHC class II B using reference Strand-Mediated Conformational Analysis (RSCA).

BackgroundThe Major Histocompatibility Complex (MHC) is a cluster of genes involved in the vertebrate immune system and includes loci with an extraordinary number of alleles. Due to the complex evolution of MHC genes, alleles from different loci within the same MHC class can be very similar and therefore difficult to assign to separate loci. Consequently, single locus amplification of MHC genes is hard to carry out in species with recently duplicated genes in the same MHC class, and multiple MHC loci have to be genotyped simultaneously. Since amplified alleles have the same length, accurate genotyping is difficult. Reference Strand-Mediated Conformational Analysis (RSCA), which is increasingly used in studies of natural populations with multiple MHC genes, is a genotyping method capable to provide high resolution and accuracy in such cases.FindingsWe adapted the RSCA method to genotype multiple MHC class II B (BLB) genes in black grouse (Tetrao tetrix), a non-model galliform bird species, using a 96-Capillary Array Electrophoresis, the MegaBACE™ 1000 DNA Analysing System (GE Healthcare). In this study we used fluorescently labelled reference strands from both black grouse and hazel grouse and observed good agreement between RSCA and cloning/sequencing since 71 alleles were observed by cloning/sequencing and 76 alleles by RSCA among the 24 individuals included in the comparison. At the individual level however, there was a trend towards more alleles scored with RSCA (1-6 per individual) than cloning/sequencing (1-4 per individual). In 63% of the pair-wise comparison, the identical allele was scored in RSCA as in cloning/sequencing. Nine out of 24 individuals had the same number of alleles in RSCA as in cloning/sequencing. Our RSCA protocol allows a faster RSCA genotyping than presented in many other RSCA studies.ConclusionsIn this study, we have developed the RSCA typing method further to work on a 96-Capillary Array Electrophoresis (MegaBACE™ 1000). Our RSCA protocol can be applied to fast and reliable screening of MHC class II B diversity of black grouse populations. This will facilitate future large-scale population studies of black grouse and other galliformes species with multiple inseparable MHC loci.

Modelling post-release survival of reintroduced Red-billed Curassows Crax blumenbachii

Modelling post-release survival probabilities of reintroduced birds can help inform ‘soft-release’ strategies for avian reintroductions that use captive-bred individuals. We used post-release radiotelemetry data to estimate the survival probabilities of reintroduced captive-bred Red-billed Curassow Crax blumenbachii, a globally threatened Cracid endemic to the Brazilian Atlantic Rainforest. Between August 2006 and December 2008, 46 radiotagged Curassows from the Crax Brazil breeding centre were reintroduced to the Guapiaçu Ecological Reserve (REGUA), Rio de Janeiro state, Brazil, in seven different cohorts. Reintroduced birds were most vulnerable during the first 12 months post-release from natural predation, domestic dogs and hunting. Annual post-release survival probability was high (75%) compared with published estimates for other Galliform species. However, when considering survival in all birds transported to REGUA (some birds died before release or were retained in captivity) and not only post-release survival, ϕ in this study was closer to estimates for other species (60%). The duration of the pre-release acclimatization period within the soft-release enclosure and the size of the released cohorts both positively influenced post-release survival of reintroduced Curassows. Our results are relevant to future Cracid reintroductions and highlight the importance of utilizing post-release monitoring data for evidence-based improvements to soft-release strategies that can significantly enhance the post-release survival of captive-bred birds.

Cooperative breeding by Buff-throated Partridge Tetraophasis szechenyii: a case in the Galliformes

Cooperative breeding is rarely found in the Galliformes. We report a case in Buff-throated Partridge Tetraophasis szechenyii, a sexually monochromatic Galliform species endemic to western China. A total of 68 groups were detected in the 2006–2009 breeding seasons, and cooperative breeding was observed to be facultative, with 64.7% (44/68) of pairs having up to three helpers of either sex (but predominantly male). Groups usually remained stable within the breeding season. Sampling revealed that all adult members exhibited behavioral efforts in terms of brooding, vigilance, and territorial display (including calling and fighting), with helpers especially focusing on territorial fighting. No evidence was found to suggest that the presence of helpers significantly enhanced a group’s breeding success and productivity compared to unaided pairs. Further studies, particularly intraspecific comparative analyses (for example, between populations or between sites) and those measuring the relatedness between helpers and other group members, are needed to understand the occurrence of cooperative breeding in this species.

Broad spectrum reactivity versus subtype specificity—Trade-offs in serodiagnosis of influenza A virus infections by competitive ELISA

Avian influenza viruses (AIVs) of the H5 and H7 subtypes can cause substantial economic losses in the poultry industry and are a potential threat to public health. Serosurveillance of poultry populations is an important monitoring tool and can also be used for control of vaccination campaigns. The purpose of this study was to develop broadly reactive, yet subtype-specific competitive ELISAs (cELISAs) for the specific detection of antibodies to the notifiable AIV subtypes H5 and H7 as an alternative to the gold standard haemagglutination inhibition assay (HI). Broadly reacting monoclonal competitor antibodies (mAbs) and genetically engineered subtype H5 or H7 haemagglutinin antigen, expressed and in vivo biotinylated in insect cells, were used to develop the cELISAs. Sera from galliform species and water fowl ( n = 793) were used to evaluate the performance characteristics of the cELISAs. For the H5 specific cELISA, 98.1% test sensitivity and 91.5% test specificity (97.7% and 90.2% for galliforms; 98.9% and 92.6% for waterfowl), and for the H7 cELISA 97.3% sensitivity and 91.8% specificity (95.3% and 98.9% for galliforms; 100% and 82.7% for waterfowl) were reached when compared to HI. The use of competitor mAbs with broad spectrum reactivity within an AIV haemagglutinin subtype allowed for homogenous detection with high sensitivity of subtype-specific antibodies induced by antigenically widely distinct isolates including antigenic drift variants. However, a trade-off regarding sensitivity versus nonspecific detection of interfering antibodies induced by phylo- and antigenically closely related subtypes, e.g., H5 versus H2 and H7 versus H15, must be considered. The observed intersubtype antibody cross-reactivity remains a disturbance variable in AIV subtype-specific serodiagnosis which negatively affects specificity.

A survey of galliform monitoring programs and methods in the United States and Canada

AbstractWe mailed survey questionnaires to 62 upland game bird managers in the United States and Canada in 2004. We received questionnaires from 47 of the 62 (76%) upland game bird managers that were contacted and 43 (91%) respondents provided information on how monitoring data were used. Responses indicated monitoring programs (population trends and/or harvest monitoring) for 23 species of Galliformes, with 145 ± 208 personnel days/year devoted to monitoring. Estimating general population trends (e.g. up or down) was the most frequent objective (N = 41; 95%) of survey data. Other applications of data included assessments of hunting activity, evaluations of regional programs and reviews of conservation status related to Endangered Species Act petitions. The majority of respondents (i.e. 63%) with monitoring programs considered the programs within their states to be effective with respect to their objectives. Many states rely upon hunter surveys, harvest data or road counts to access demographic and population data to address major conservation and management issues. The relevance of these issues is growing and agencies must respond with management recommendations, but often must do so with limited data on the status of their populations. Comprehensive monitoring should be a major component of conservation and management planning for upland gamebird populations particularly as a tool to track and evaluate the effectiveness of management actions and inform management options.

Habitat preferences of high-altitude Galliformes in Sagarmatha National Park, Nepal

In the Sagarmatha National Park, Nepal, Himalayan species of Galliformes are poorly studied and their present status is unknown. We studied the distribution of three high-altitude species: Himalayan monal, blood pheasant, and Tibetan snowcock, comparing birds' distribution in relation to altitude and habitat in spring and autumn 2007. Our study area was at 3300–5000 m a.s.l., characterized by subalpine vegetation. A structural description of vegetation types was made on the basis of main habitat features. We observed two different patterns across the year. Group size differences are common during spring and autumn. Variation in habitat use and altitudinal ranges are evident in the snowcock and blood pheasant. The Himalayan monal distribution was influenced by anthropogenic resources.

Comparative Pathology of Select Agent Influenza A Virus Infections

Influenza A virus infections may spread rapidly in human populations and cause variable mortality. Two of these influenza viruses have been designated as select agents: 1918 H1N1 virus and highly pathogenic avian influenza (HPAI) virus. Knowledge of the pathology of these virus infections in humans, other naturally infected species, and experimental animals is important to understand the pathogenesis of influenza, to design appropriate models for evaluation of medical countermeasures, and to make correct diagnoses. The most important complication of influenza in humans is viral pneumonia, which often occurs with or is followed by bacterial pneumonia. Viremia and extrarespiratory disease are uncommon. HPAI viruses, including HPAI H5N1 virus, cause severe systemic disease in galliform species as well as in anseriform species and bird species of other orders. HPAI H5N1 virus infection also causes severe disease in humans and several species of carnivores. Experimental animals are used to model different aspects of influenza in humans, including uncomplicated influenza, pneumonia, and virus transmission. The most commonly used experimental animal species are laboratory mouse, domestic ferret, and cynomolgus macaque. Experimental influenza virus infections are performed in various other species, including domestic pig, guinea pig, and domestic cat. Each of these species has advantages and disadvantages that need to be assessed before choosing the most appropriate model to reach a particular goal. Such animal models may be applied for the development of more effective antiviral drugs and vaccines to protect humans from the threat of these virus infections.

The link between metabolic rate and body temperature in galliform birds in thermoneutral and heat exposure conditions: The classical and phylogenetically corrected approach

Three galliform species (grey partridges, ring-necked pheasants, and king quail) were involved in body temperature and resting metabolic rate measurements over a broad range of ambient temperatures (20–45 °C). At thermoneutrality, inter-species differences in colonic temperature, as well as in metabolic rate, were observed. During heat exposure, all species reacted by elevating their body temperatures above 44 °C, thereby inducing temporary hyperthermia. Heat-stressing birds resulted in a slightly increased metabolic rate in king quail, but not in partridges and pheasants. Based on data of body temperature and weight specific (per body mass unit) basal metabolic rate among ten species of Galliformes order, classical and phylogenetically corrected analyses of covariation between these two physiological traits were performed. The scaling of body temperature to body mass, revealed a significant exponent of: −0.0062 and −0.0080 for conventional and phylogenetical methods, respectively. In the analyzed species, a strong positive relationship between residuals of body mass values between body temperature and metabolic rate were found. The results obtained may show a plausible evolutionary link between these traits in galliform birds.

Estimation of divergence times for major lineages of galliform birds: Evidence from complete mitochondrial genome sequences

Determining an absolute timescale for avian evolutionary history has been recently challenged by the relaxed molecular clock methods, that rates of molecular evolution can vary significantly among organisms. In this study, we used relaxed molecular clocks to date the divergence of major lineages of Galliformes based on complete mitochondrial genomes. A nucleotide dataset of 13 concatenated protein-coding genes from 22 species of Galliformes was used to investigate the evolutionary divergences within the group. Using Gallus bravardi, Schaubortyx and Gallinuloides fossils as calibration points, divergence times analyses were performed with four relaxed molecular clock methods as follows: (1) Bayesian method of Multidivtime; (2) Bayesian Markov chain Monte Carlo (MCMC) analysis of the Bayesian evolutionary analysis by sampling trees (BEAST); (3) local rate minimum deformation method (LRMD) of TREEFINDER; and (4) nonparametric rate smoothing (NPRS) of TREEFINDER. The various relaxed clock methods all indicated that (1) Megapodiidae originated in the Late Cretaceous; (2) Numididae, Phasianidae, Arborophilinae and Coturnicinae originated in the Eocene of Palaeogene; (3) Pavoninae and Gallininae originated at the Eocene-Oligocene boundary; (4) Phasianinae and Meleagridinae originated in the Oligocene; (5) divergence times estimation among most genera of Phasianidae were much older than those of the previous studies. Our results might provide a more likely time scale for evolutionary history of the galliform birds.

A mitogenomic perspective on the ancient, rapid radiation in the Galliformes with an emphasis on the Phasianidae.

BackgroundThe Galliformes is a well-known and widely distributed Order in Aves. The phylogenetic relationships of galliform birds, especially the turkeys, grouse, chickens, quails, and pheasants, have been studied intensively, likely because of their close association with humans. Despite extensive studies, convergent morphological evolution and rapid radiation have resulted in conflicting hypotheses of phylogenetic relationships. Many internal nodes have remained ambiguous.ResultsWe analyzed the complete mitochondrial (mt) genomes from 34 galliform species, including 14 new mt genomes and 20 published mt genomes, and obtained a single, robust tree. Most of the internal branches were relatively short and the terminal branches long suggesting an ancient, rapid radiation. The Megapodiidae formed the sister group to all other galliforms, followed in sequence by the Cracidae, Odontophoridae and Numididae. The remaining clade included the Phasianidae, Tetraonidae and Meleagrididae. The genus Arborophila was the sister group of the remaining taxa followed by Polyplectron. This was followed by two major clades: ((((Gallus, Bambusicola) Francolinus) (Coturnix, Alectoris)) Pavo) and (((((((Chrysolophus, Phasianus) Lophura) Syrmaticus) Perdix) Pucrasia) (Meleagris, Bonasa)) ((Lophophorus, Tetraophasis) Tragopan))).ConclusionsThe traditional hypothesis of monophyletic lineages of pheasants, partridges, peafowls and tragopans was not supported in this study. Mitogenomic analyses recovered robust phylogenetic relationships and suggested that the Galliformes formed a model group for the study of morphological and behavioral evolution.

A cross-sectional survey of influenza A infection, and management practices in small rural backyard poultry flocks in two regions of New Zealand

AIMS: To obtain baseline data on the management of small non-commercial backyard poultry flocks, in two rural regions of New Zealand, to investigate potential transmission pathways for avian influenza (AI), and to investigate the presence of AI in these flocks. METHODS: During August–October 2006 a questionnaire was sent to 105 farms in the Bay of Plenty and Wairarapa with poultry flocks comprising fewer than 50 chickens, located near wetlands where AI virus had been detected previously in wild ducks. Information was collected on the number and species of poultry reared, opportunities for interaction between wild birds and poultry, farm biosecurity measures, and health status of poultry. Between September and November 2006, blood and tracheal/cloacal swabs were collected from poultry on a subset of 12 high-risk farms in each location. Influenza A-specific antibodies in sera were assayed using ELISA, and positive sera were further tested for the presence of H5 and H7 subtype-specific antibodies, using haemagglutination inhibition (HI) assay. The presence of influenza A virus in swabs was detected using realtime reverse transcriptase-PCR (RRT-PCR). RESULTS: Returned questionnaires were received from 54 farms. Overall, 80% had only chickens, 13% chickens and ducks, and 7% had chickens and other galliform species. Nearly all (96%) kept backyard chickens for personal consumption of eggs, with a small proportion (19%) preparing birds for the table. On surveyed farms wild waterfowl were seen on pastures (70%) and/or farm waterways (46%). Waterfowl were recorded as visiting areas where domestic birds were kept on 31% of farms. Bird litter and manure were composted (94%) or buried (6%) on-farm, as were most (82%) dead birds. During the targeted cross-sectional survey of 24 farms clinical disease was not recorded in any poultry flock. Of 309 chicken sera tested, 11 (3.6%) from five farms across both regions tested positive for influenza A antibodies. In contrast, 16/54 (30%) duck sera from three farms in the Wairarapa were positive. Avian influenza H5 and H7 subtype-specific antibodies were excluded in ELISApositive sera using HI testing, and influenza A virus was not detected using RRT-PCR. CONCLUSIONS: The study confirmed that small backyard poultry flocks located near waterfowl habitats were exposed to non-notifiable low-pathogenic AI viruses. Findings indicate a number of potential risk pathways for the transmission of AI viruses between wild birds and non-commercial poultry, and hence the need for continued surveillance for AI in backyard flocks and wild birds in New Zealand.

Improvement on molecular sex identification primers for Passeriform bird species

The primer pair sex1/sex2, which can be widely applied for sex identification in Galliform species, was used to determine the sex of 17 Passeriform species. As CHD-W fragments tended to be preferentially amplified, which may cause unnecessary misidentification in bird species with little difference between CHD-Z and CHD-W, we modified sex1 and sex2, obtaining sex1' and sex-mix respectively. Primer sets were then recombined to conduct sex identification. After testing several Passeriforme birds of known sex, we found that the primer pair sex1'/sex2 was better at limiting the preferential amplification of CHD-W fragments. As they are being frequently used in sex allocation study of Aegithalos concinnus and song learning research of Lonchura striata, we can expect more applications of this primer pair to further studies in Passeriformes.

Phylogeny of major lineages of galliform birds (Aves: Galliformes) based on complete mitochondrial genomes

Complete mitochondrial DNA sequences have been used successfully to estimate phylogenetic relationships among animal taxa, and for studies of population genetics and molecular evolution. We made phylogenetic analyses of 22 species of Galliformes, with two species of Anseriformes as outgroups, using maximum likelihood (ML), maximum parsimony (MP) and Bayesian inference (BI) methods based on the nucleotide dataset and the corresponding amino acid dataset of 13 concatenated protein-coding genes. The consensus phylogenetic trees supported monophyly of Galliformes, Phasianidae (nucleotide and amino acid: posterior probabilities 1.00 in BI, bootstrap value > 99% in ML and MP), Coturnicinae, and Gallininae (nucleotide and amino acid: posterior probabilities 1.00 in BI, bootstrap value > 85% in ML and MP), but failed to demonstrate monophyly of Pavoninae and Phasianinae. Our results also support a sister-group relationship between megapodes and all other galliforms. We found that Arborophilinae is basal to the balance of the Phasianidae. Moreover, we suggest that the turkey should be classified in the Phasianinae of Phasianidae. Although the relationships among the various lineages of the Galliformes remain controversial, these results should be useful for further study.

Interspecific brood parasitism in galliform birds

Mode of development in birds helps determine the form of brood parasitism a species exhibits. Most knowledge of precocial brood parasites comes from a single avian family, the waterfowl (Anatidae: Anseriformes). Here we review cases of interspecific brood parasitism (IBP) in a second group of precocial birds, the order Galliformes. IBP is uncommon but taxonomically widespread, occurring in at least 11 species and in four of five galliform families. By far the most common brood parasite is the Ring‐necked PheasantPhasianus colchicus. Hosts were generally other ground‐nesting precocial species. It is unclear whether the absence of IBP in the Cracidae (Guans, Curassows, and Chachalacas) is due to the paucity of research on tropical gamebirds or because tropical birds such as the Cracidae may be less likely to practise IBP. Galliform birds mirror the trend found in ducks in which virtually all species that parasitize heterospecifics are also conspecific brood parasites (CBP). This association supports the hypothesis that IBP as an adaptive tactic or strategy may evolve from CBP. Alternatively, or additionally, egg‐dumping may represent reproductive error on the part of females, such that concordance between CBP and IBP could be a byproduct of having sufficient knowledge of breeding biology only for a subset of galliform species.