Carp Species Research Articles

Impacts of Some Trace Metals in Cyprinus carpio (Linnaeus,1758) and Tor soro (Valenciennes, 1842) on Human Health.

Although fish are rich sources of various trace metals in their bodies, when these metals exceed health safety limits, it becomes a global concern. Fish exposed to trace metal pollution in their aquatic ecosystems can lead to bioaccumulation and transfer to consumers. Monitoring trace metal contamination of fish food is crucial because they can reach humans through the aquatic food chain, resulting in health problems. Therefore, a first attempt was made for the analysis of trace metals in two cyprinid fish species, namely Cyprinus carpio (Common carp) and Tor soro (Mahseer), which were abundantly found in Khanozai Dam and assessing their potential health impacts when consumed by humans. A total of 200 fish specimens were collected from this area. These specimens were divided equally between the two carp species, with 100 individuals from each species. The fish specimens were collected over 12months, from June 2020 to May 2021 to determine the concentration of some trace metals, i.e. Manganese (Mn), Calcium (Ca), Iron (Fe), Potassium (K), and Zinc (Zn), Sodium (Na), Phosphorus (P), Magnesium (Mg), and Chlorine (Cl) using a Flame Atomic Absorption Spectrophotometer with a multi-element hollow cathode lamp. The obtained results from the trace metal analysis of Cyprinus carpio and Tor soro in this study revealed that both calcium and phosphorus were present in higher concentrations. However, iron, manganese, and zinc were not detected in both species. Additionally, magnesium was also not detected in C. carpio. For Cyprinus carpio, the arrangement was Ca > P > K > Na > Cl, while for Tor soro, it was Ca > P > Na > K > Cl > Mg in decreasing order. The health risks associated with fish consumption were assessed using factors like estimated daily intake (EDI), Target Hazard Quotient (THQ), and Total Target Hazard Quotient (TTHQ). The THQ values for all analyzed trace metals in both carp species exceeded the maximum value of 1.0, except for Cl in Tor soro. This suggests the potential for various health-related diseases in regular consumers due to their elevated levels. In both species, the TTHQ factor also surpassed the standard value of 1.0, indicating an acute health risk for humans. Although EDI values did not surpass the recommended daily allowance (RDA), continuous consumption of these contaminated fish from this area could lead to severe health complications. Therefore, these results strongly indicated that the elevated levels of trace metals found in the highly abundant cyprinid species of Khanozai Dam could pose serious health-related issues for the local inhabitants.

Utility of Mitochondrial Atpase 8 Gene Sequence in Identification of Indian Carps

Identification of fish species is essential for better broodstock management as well as for the detection of unintentional or fraudulent species substitutions in the market place. Twenty seven individuals from 4 Indian carp species were analyzed to assess the utility of mitochondrial ATPase 8 gene sequences in identifying four cultivable carp species i.e, Labeo rohita, Catla catla, Cirrhinus mrigala and Labeo fimbriatus from India. Results of sequencing and alignment of the full length ATPase 8 gene of the four species revealed that there were enough species specific signatures which could be used for differentiating these four species. The phylogenetic analysis of Indian carp species by the Kimura 2-parameter method showed that each species forms a separate clade, and L. rohita is more closely related to L. fimbriatus than C. catla and C. mrigala. This study using only a very short sequence of ATPase 8 gene may help in identification of carp species as well as detection of fraudulent substitution of fish species.

Water Requirement and Efficacy of a Prototype Hatchery for Selective Breeding of Rohu, <i>Labeo rohita</i>

A prototype hatchery was constructed at Central Institute of Freshwater Aquaculture (CIFA), Kausalyaganga, Bhubaneswar, India to produce spawn of fullsib families of rohu (Labeo rohita) carp and mass production of genetically improved rohu spawn. While following combined selection method, fullsib families have to be produced and reared separately from fertilized eggs to fingerlings i.e., till taggable size for individual tagging. The specialized hatchery was developed for individual fullsib family spawn production i.e., fertilized eggs to spawn. Water requirement study was performed for full sib family production as well as for mass scale spawn production and, it was worked out to be 10.54 and 81.07 m3 for one fullsib family and a million of spawn production, respectively. The specialized hatchery improved the production of fullsib families and spawn recovery percentage to a greater extent. Recovery of fullsib families increased from 70-82% to 94-100% in different year classes. In the present paper, efficacy of the hatchery for fullsib family and mass scale spawn production in selective breeding programme of rohu for last 19 years is discussed. The same model of hatchery can also be utilized for selective breeding programme for any other carp species.

Investigation of mucus cell change in gills of van fish (Alburnus tarichi, Güldenstädt, 1814) treated with fluvalinate

ABSTRACT The Van fish is an endemic carp species living in Lake Van, one of the largest soda lakes in the world. Fluvalinate is a pesticide used extensively in the Lake Van basin. In the present study, the effects of sublethally applied fluvalinate on mucous cells in Van fish gill tissue after 48, 72, and 96 h were determined histochemically. Histopathological lesions were observed in the gills of the fish exposed to fluvalinate in a time-dependent manner. The concentrations of neutral glycoconjugates, acidic glycoconjugates, sulfated acidic glycoconjugates, and strongly acidic glycoconjugates in the mucous cells increased in the gills of the Van fish during the exposure period. At the same time, the area and number of mucous cells increased during fluvalinate exposure. As a result, fluvalinate caused an increase in mucous secretion and content in Van fish gills.

Effects of short-term water velocity stimulation on the biochemical and transcriptional responses of grass carp (Ctenopharyngodon idellus).

Since 2011, ecological operation trials of the Three Gorges Reservoir (TGR) have been continuously conducted to improve the spawning quantity of the four major Chinese carp species below the Gezhouba Dam. In particular, exploring the effects of short-term water velocity stimulation on ovarian development in grass carp (Ctenopharyngodon idellus) is essential to understand the response of natural reproduction to ecological flows. We performed ovary histology analysis and biochemical assays among individuals with or without stimulation by running water. Although there were no obvious effects on the ovarian development characteristics of grass carp under short-term water velocity stimulation, estradiol, progesterone, follicle-stimulating hormone (FSH), and triiodothyronine (T3) concentrations were elevated. Then, we further explored the ovarian development of grass carp under short-term water velocity stimulation by RNA sequencing of ovarian tissues. In total, 221 and 741 genes were up- or downregulated under short-term water velocity stimulation, respectively, compared to the control group. The majority of differentially expressed genes (DEGs) were enriched in pathways including ABC transporters, cytokine-cytokine receptor interaction, ECM-receptor interaction, and steroid hormone biosynthesis. Important genes including gpr4, vtg1, C-type lectin, hsd17b1, cyp19a1a, cyp17a1, and rdh12 that are involved in ovarian development were regulated. Our results provide new insights and reveal potential regulatory genes and pathways involved in the ovarian development of grass carp under short-term water velocity stimulation, which may be beneficial when devising further ecological regulation strategies.

Species Diversity of Fish and consumption in Nan River in Bang Krathum district, Phitsanulok province, Thailand

The fishery is an essential part of Thai culture and provides the rural population with a significant amount of animal protein. This research targeted the examination of fish species, the status of fish capture and consumption along the Nan River in the Bang Krathum district, Phitsanulok province, Thailand. Between November 2019 and April 2020, three sites were sampled along the river, and four sites were sampled in the local markets. A total of 35 individuals including fishermen, fish traders, and governmental officers involved in the region's fish resource management were subjected to purposive sampling. The data analysis was undertaken utilizing methods such as mean, frequency and percentage. The findings revealed 28 fish species across 11 families and six orders. The family Cyprinidae in the order Cypriniformes had the highest number of species (10 species). The number of fish species found in the local markets (19 species) was higher than those found in the river (17 species). In terms of consumption, the majority of fishermen and fish traders were female (65.71% and 84.62%, respectively). However, 95.00% of them lacked certification for fishing in public areas. The preferred method of fishing was floating netting, with predominant catch of carp species recorded at least twice a week. Compared to previous figures, productivity has decreased indicating a need for adequate training and knowledge about natural resource management and stocking enhancement.

Market integration and market leadership: Evidence for cyprinoid markets in China

Cyprinoids are important for food security in China, and information about the degree of integration of the markets for cyprinoids is valuable to Chinese policy makers in their attempt to regulate the fisheries and aquaculture industry. This study investigates horizontal and vertical market integration of three species of carps (grass, common and silver) at two stages of the value chain (ex-farm and retail). The study implements a Vector-Error-Correction model, and it uses monthly data ranging from 2015 to 2019. The study shows that with the exception of the markets for common and grass carps at the retail stage, markets are, at best, partially integrated. Horizontally, only the common and the grass carps show significant price transmission. At both value chain stages, the price relationship is bidirectional, and no clear-cut market leadership exists. Vertically, the price relationship is also bidirectional for the common and the grass carp, but the silver carp shows no significant market integration across the value chain.

Histopathological evaluation of the infection elicited by three species of Myxobolus (Cnidaria: Myxosporea), with identification of Myxobolusxiaoganensis n. sp. isolated from the silver carp Hypophthalmichthys molitrix

Several parasites infecting the Asian carp species have been broadly spread along with the global fish trade. However, the diversity of specific parasite groups and their pattern of parasitism remain insufficiently elucidated even in their native regions. Here, we conducted a holistic identification and histological analysis of three Myxobolus species. The oblate myxospores of the first isolates were found infecting the spleen of silver carp Hypophthalmichthys molitrix, measuring 6.4 ± 0.4 (5.4–7.1) μm in length, 8.2 ± 0.4 (7.5–9.0) in width, and 5.9 ± 0.3 (5.2–6.2) in thickness. They were morphologically distinct from other congeners and regarded as a novel species, Myxobolus xiaoganensis n. sp. For the second isolates, we associated the formation of round plasmodia on the gill raker of silver carp with Myxobolus allotypica Chen, 1998. The third isolates, encapsulated in the intestinal serosa membrane of the grass carp Ctenopharyngodon idella, was proved to be conspecific to Myxobolus huasaensis Chen, 1998. While M. xiaoganensis n. sp. and M. huasaensis exhibited distinct origins, with genetic differences exceeding 4% from other congeners, M. allotypica displayed complete genetic congruence with an item available in Genbank. Histologically, myxospores of M. xiaoganensis n. sp. were scattered in the spleen, and the branchial and intestinal infections of M. allotypica and M. huasaensis were determined, respectively. Phylogenetic analysis demonstrated a non-monophyletic origin of both the Thelohanellus and Myxobolus genera, with a remarkable association of host affinity with myxosporean clustering.

Amino Acid Variation at the Mitochondrial Binding Site of Antimycin A is Proposed to Reflect Sensitivity and Toxicity Differences among Fish Species

To better understand differential sensitivities among fish species to the piscicidal compound Antimycin-A (ANT-A), we hypothesized that variations in amino acids at the ANT-A binding site may reflect toxicity differences. Protein sequences for six motifs comprising the ANT-A binding site were obtained and compared for invasive carp species (N = 515) and seven non-target species (N = 277); a consensus was delineated from each species. The carp species, Common Carp (Cyprinus carpio), Silver Carp (Hypophthalmichthys molitrix), Bighead Carp (Hypophthalmichthys nobilis), Grass Carp (Ctenopharyngodon idella), and Black Carp (Mylopharyngodon piceus), showed the same amino acids at the site; thus, it was termed the carp consensus motif sequence (CCM). Channel Catfish (Ictalurus punctatus) showed the most amino acid polymorphisms, with three motifs 96–100% different from CCM. Within a species, Bluegill (Lepomis macrochirus) and Fathead Minnow (Pimephales promelas) variation per motif was most dissimilar (46.7% and 21.6%, respectively). Organismal mortality data from the literature indicated Yellow Perch (Perca flavescens), Walleye (Sander vitreus), and American Gizzard Shad (Dorosoma cepedianum) to be most sensitive to the piscicide, Catfish least sensitive, and all others intermediate. The protein sequence variations of the binding site appeared to be in accord with organismal sensitivity categories when they differed from the CCM; the motifs in Gizzard Shad and Walleye were the same as in CCM. The physical/chemical nature of ANT-A is important to consider in organismal response comparisons. This cellular approach of studying ANT-A binding at its target enzyme is a non-destructive way to predict piscicidal efficacy of ANT-A against fishes of interest, informs management decisions in control efforts for invasives, and can be used to forecast effects on sympatric species.

Analysis of Supply Chain and Fish Marketing in Nawalparasi (East), Nepal

Study was conducted during October 2021 to November 2021, to investigate the supply chain, present market situation, marketing constrains, marketing margins, and the consumer’s behavior and purchasing pattern towards the fish in Nawalparasi (East) district of Nepal. Fish producers, traders and consumers were surveyed through semi-structure questionnaire. Five fish Producers, eight wholesalers, twelve retailers and twenty consumers were randomly surveyed. Among 20 traders majority of the traders belonging to the Madhesi and Tharu community. Finding of the study revealed that fish trading almost dominated by male (84%). All the respondents involved in fish trading more than 30 years old. there was no specific marketing channel used by the farmers, 55.65% of total fish produce is sold to the wholesalers, 32.49% to the retailers and 11.86% is sold to the customers directly by the fish farmers. The marketing margin of Nepalese live fish Rohu and Common Carp was higher than other species of fish in the Nawalparasi (East). Nepali fish Rohu and common carp fetch highest price Rs 425/kg and Pangasius fetch lowest price Rs. 280/kg. Nepalese fish were mostly packed in plastic crates and transported by pick up van. Indian average fish sizes were 2.91 kg and Nepali average fish sizes were 1.91 kg. Among the major carp species; the most preferred one by the consumers was Rohu followed by Bhakur.

Value chain analysis of carp fish seed farms in some selected areas of Mymensingh district in Bangladesh

This study is an attempt to analyze the prevailing value chain of fish seed farms in some selected areas of Mymensingh district in Bangladesh. Data were collected from 280 fish seed farms owners (56) and traders (224) covering four Upazilas of Mymensingh district namely Mymensingh Sadar, Gauripur, Muktagacha, and Trishal from the period of July 2019 to October 2019 through purposive random sampling technique. Three important fish seed species of carp; namely Rui, Catla, Mrigel were selected to address the following objectives: typical value chain map and marketing system of fish seed farms, determination of the net value addition of fish seed production and marketing, and problems impacting different actors in the value chain. Total net marketing margin per 10 Kg. spawn and per 100000 pieces’ fingerlings are Tk. 20204 (190.43 USD) and Tk. 43148 (406.69 USD), respectively. Among all three intermediaries, the net marketing margin of the retailer is the highest. A large percentage of value addition is covered by paiker for both cases and that is 20.85% and 31.2%. The study finds that fish seed farms with hatcheries are more profitable than fish seed farms with nursery. The yearly net return from spawn production in the hatchery is Tk. 4324775 (40763.15 USD) and from fingerling production in the nursery is Tk. 2388126 (22509.27 USD). The owners and traders have currently been facing some difficulties. Lack of information about good quality seed, the higher price of inputs, and lack of extension service is some of the examples. If these problems could be solved, fish seed farms and market intermediaries could earn a higher profit than the existing level.

Interplay of gene expression and regulators under salinity stress in gill of Labeo rohita

BackgroundLabeo rohita is the most preferred freshwater carp species in India. The concern of increasing salinity concentration in freshwater bodies due to climate change may greatly impact the aquatic environment. Gills are one of the important osmoregulatory organs and have direct contact with external environment. Hence, the current study is conducted to understand the gill transcriptomic response of L. rohita under hypersalinity environment.ResultsComprehensive analysis of differentially expressed long non-coding RNAs (lncRNAs), microRNAs (miRNAs) and mRNAs was performed in gills of L. rohita treated with 2, 4, 6 and 8ppt salinity concentrations. Networks of lncRNA-miRNA-mRNA revealed involvement of 20, 33, 52 and 61 differentially expressed lncRNAs, 11, 13, 26 and 21 differentially expressed miRNAs in 2, 4, 6 and 8ppt groups between control and treatment respectively. These lncRNA-miRNA pairs were regulating 87, 214, 499 and 435 differentially expressed mRNAs (DE mRNAs) in 2, 4, 6 and 8ppt treatments respectively. Functional analysis of these genes showed enrichment in pathways related to ion transportation and osmolyte production to cope with induced osmotic pressure due to high salt concentration. Pathways related to signal transduction (MAPK, FOXO and phosphatidylinositol signaling), and environmental information processing were also upregulated under hypersalinity. Energy metabolism and innate immune response pathways also appear to be regulated. Protein turnover was high at 8ppt as evidenced by enrichment of the proteasome and aminoacyl tRNA synthesis pathways, along with other enriched KEGG terms such as apoptosis, cellular senescence and cell cycle.ConclusionAltogether, the RNA-seq analysis provided valuable insights into competitive endogenous (lncRNA-miRNA-mRNA) regulatory network of L. rohita under salinity stress. L. rohita is adapting to the salinity stress by means of upregulating protein turnover, osmolyte production and removing the damaged cells using apoptotic pathway and regulating the cell growth and hence diverting the essential energy for coping with salinity stress.

Identification of differentially expressed genes and SNPs linked to harvest body weight of genetically improved rohu carp, Labeo rohita.

In most of the aquaculture selection programs, harvest body weight has been a preferred performance trait for improvement. Molecular interplay of genes linked to higher body weight is not elucidated in major carp species. The genetically improved rohu carp with 18% average genetic gain per generation with respect to harvest body weight is a promising candidate for studying genes' underlying performance traits. In the present study, muscle transcriptome sequencing of two groups of individuals, with significant difference in breeding value, belonging to the tenth generation of rohu carp was performed using the Illumina HiSeq 2000 platform. A total of 178 million paired-end raw reads were generated to give rise to 173 million reads after quality control and trimming. The genome-guided transcriptome assembly and differential gene expression produced 11,86,119 transcripts and 451 upregulated and 181 downregulated differentially expressed genes (DEGs) between high-breeding value and low-breeding value (HB & LB) groups, respectively. Similarly, 39,158 high-quality coding SNPs were identified with the Ts/Tv ratio of 1.23. Out of a total of 17 qPCR-validated transcripts, eight were associated with cellular growth and proliferation and harbored 13 SNPs. The gene expression pattern was observed to be positively correlated with RNA-seq data for genes such as myogenic factor 6, titin isoform X11, IGF-1 like, acetyl-CoA, and thyroid receptor hormone beta. A total of 26 miRNA target interactions were also identified to be associated with significant DETs (p-value < 0.05). Genes such as Myo6, IGF-1-like, and acetyl-CoA linked to higher harvest body weight may serve as candidate genes in marker-assisted breeding and SNP array construction for genome-wide association studies and genomic selection.

Can the regulation of Golden Inland Waterways meet the needs of navigation, flood control, and ecology? A model-based case study

In recent years, the ecological functions of inland waterways have received much attention. Roughness elements have been used in waterway regulations (WRs) to guard the riverbed and improve physical habitat. However, the response of aquatic organisms to regulations varies widely. It is controversial whether inland waterway regulations can achieve the sustainable function of navigation, flood control and ecological needs.This paper evaluated the influence of WRs on aquatic habitat by using the physical habitat simulation models combining a 2D hydrodynamic model and habitat models. Compared with a reference model with no-engineering, the habitat suitability of zoobenthos, four major species of Chinese carp's spawning ground and the Yangtze finless porpoise at different flow discharges were simulated under different densities of river training structures and types of roughness elements. Results showed that the implementation of the WRs can adjust the distribution of the suitability of aquatic habitats, but does not change the pattern of suitability with discharge. The response of biological habitats to regulation projects varied by species. Regulation can maintain the stable habitat during dry and flood periods, reducing the effect of extreme weather on aquatic organisms. Roughness elements are not suitable for large-scale use in WRs. The “win-win” agreement between economic and ecological issues can be obtained through pre-optimization.

Isolation of Aeromonas salmonicida subspecies salmonicida from aquaculture environment in India: Polyphasic identification, virulence characterization, and antibiotic susceptibility

This study reports the polyphasic identification, characterization of virulence potential, and antibiotic susceptibility of Aeromonas salmonicida subspecies salmonicida COFCAU_AS, isolated from an aquaculture system in India. The physiological, biochemical, 16s rRNA gene sequencing and PAAS PCR test identified the strain as Aeromonas salmonicida. The MIY PCR tests established the subspecies as ‘salmonicida’. The in vitro tests showed the isolated bacterium as haemolytic with casein, lipid, starch, and gelatin hydrolysis activity, indicating its pathogenic attributes. It also showed the ability to produce slime and biofilm, and additionally, it possessed an A-layer surface protein. In vivo pathogenicity test was performed to determine the LD50 dose of the bacterium in Labeo rohita fingerlings (14.42 ± 1.01 g), which was found to be 106.9 cells fish−1. The bacteria-challenged fingerlings showed skin lesions, erythema at the base of the fins, dropsy, and ulcer. Almost identical clinical signs and mortalities were observed when the same LD50 dose was injected into other Indian major carp species, L. catla and Cirrhinus mrigala. Out of the twelve virulent genes screened, the presence of nine genes viz., aerA, act, ast, alt, hlyA, vapA, exsA, fstA, and lip were detected, whereas ascV, ascC, and ela genes were absent. The A. salmonicida subsp. salmonicida COFCAU_AS was resistant to antibiotics such as penicillin G, rifampicin, ampicillin, and vancomycin while highly sensitive to amoxiclav, nalidixic acid, chloramphenicol, ciprofloxacin, and tetracycline. In summary, we have isolated a virulent A. salmonicida subsp. salmonicida from a tropical aquaculture pond which can cause significant mortality and morbidity in Indian major carp species.

Disease ecology and host range of Cyprinid herpesvirus 3 (CyHV-3) in CyHV-3 endemic lakes of North America.

Cyprinid herpesvirus-3 (CyHV-3) is an important pathogen of common carp (Cyprinus carpio, carp) causing significant economic and ecological impacts worldwide. The recent emergence of CyHV-3 in the Upper Midwest region of the United States has raised questions related to the disease ecology and host specificity of CyHV-3 in wild carp populations. To determine the prevalence of CyHV-3 in wild populations of fishes in Minnesota, we surveyed five lakes in 2019 in which the virus was known to have caused mass mortality events in carp from 2017 to 2018. A total of 28 species (n=756 total fish) of native fishes and 730 carp were screened for the presence of CyHV-3 DNA using specific qPCR. None of the native fish tissues tested positive for CyHV-3 although the prevalence of CyHV-3 in carp was 10%-50% in the five lakes. A single lake (Lake Elysian) with a 50% DNA detection rate and evidence of ongoing transmission and CyHV-3-associated mortality was surveyed again in 2020 from April to September. During this period, none of the tissues from 24 species (n=607 total fish) tested positive for CyHV-3 though CyHV-3 DNA and mRNA (indicating viral replication) was detected in carp tissues during the sampling period. CyHV-3 DNA was detected most often in brain samples without evidence of replication, potentially indicating that brain tissue is a site for CyHV-3 latency. Paired qPCR and ELISA testing for Lake Elysian in 2019-2020 identified young carp (especially males) to be the primary group impacted by CyHV-3-associated mortality and acute infections, but with no positive detections in juvenile carp. Seroprevalence of carp from Lake Elysian was 57% in 2019, 92% in April of 2020 and 97% in September 2020. These results further corroborate the host specificity of CyHV-3 to carp in mixed wild populations of fishes in Minnesota and provide additional insights into the ecological niche of CyHV-3 in shallow lake populations of carp in North America.

Prohibitin mediates the cellular invasion of spring viremia of the carp virus

Spring viremia of carp virus (SVCV) is strongly contagious and pathogenic to common carp and cyprinoid species. However, knowledge of how SVCV enters host cells is still inadequate. In this study, mass spectrometry (MS) was incorporated with tandem affinity purification (TAP) to identify host proteins that interact with SVCV glycoprotein, the main attachment protein of SVCV. Specifically, prohibitin (PHB) received the utmost attention from all the candidate proteins, and its interaction with the SVCV-G protein was confirmed by immunoprecipitation and immunofluorescence assays. Treatment with PHB-specific inhibitors or knockdown of the expression of PHB by siRNAs resulted in a marked reduction in binding and entry of SVCV on host cells, while overexpression of PHB increased SVCV attachment and invasion. Furthermore, binding of SVCV to ZF4 and FHM cells was inhibited by pre-incubating the virus with recombinant PHB protein (rPHB) or blocking the cell surface PHB with its polyclonal antibodies. In addition, overexpression of PHB on SVCV-nonpermissive Grouper spleen cells (GSs) conferred susceptibility to SVCV infection. In vivo, treatment of rPHB could significantly inhibit SVCV propagation within zebrafish and benefit the survival rate of SVCV-infected zebrafish. These results demonstrate that PHB plays a crucial role in both the attachment and entry stages of SVCV infection.

VETERINARY SANITARY EXAMINATION AND HELMINTOSIS COMPARATIVE MONITORING OF FISH OF WATER SOURCES OF KORGALZYN DISTRICT, AKMOLA REGION

This article presents the results of studies on helminthological diseases of carp species living in the rivers Nura, Tengiz, Birtaban, Shalkar, Uyaly, Sholak, Alkasor of the Korgalzhyn region. 226 studied fish samples were subjected to comparative monitoring using helminthological methods. When examining the fish of the reservoirs of the Korgalzhyn district of the Akmola region, 41.1% of the fish were found unfit for organoleptic indicators. As a result of laboratory studies, fish samples taken from the Sholak, Alkasor and Uyaly rivers were recognized as unsuitable, while the indicators of the studied fish from the Nura river and lakes Shalkar, Tengiz, Birtaban were within the normal range. According to the study, 6.4% of diplostomiasis in Lake Shalkar and 28.6% of opisthorchiasis in Lake Alkasor are affected by helminthiases. 16.6% postdiplostomiasis in the lakes Shalkar and Birtaban, 10.5% opisthorchiasis in the Nura River. In Lake Sholak, ligulase was 9.1% .

Genomic Analysis, Evolution and Characterization of E3 Ubiquitin Protein Ligase (TRIM) Gene Family in Common Carp (Cyprinus carpio)

Tripartite motifs (TRIM) is a large family of E3 ubiquitin ligases that play an important role in ubiquitylation. TRIM proteins regulate a wide range of biological processes from cellular response to viral infection and are implicated in various pathologies, from Mendelian disease to cancer. Although the TRIM family has been identified and characterized in tetrapods, but the knowledge about common carp and other teleost species is limited. The genes and proteins in the TRIM family of common carp were analyzed for evolutionary relationships, characterization, and functional annotation. Phylogenetic analysis was used to elucidate the evolutionary relationship of TRIM protein among teleost and higher vertebrate species. The results show that the TRIM orthologs of highly distant vertebrates have conserved sequences and domain architectures. The pairwise distance was calculated among teleost species of TRIMs, and the result exhibits very few mismatches at aligned position thus, indicating that the members are not distant from each other. Furthermore, TRIM family of common carp clustered into six groups on the basis of phylogenetic analysis. Additionally, the analysis revealed conserved motifs and functional domains in the subfamily members. The difference in functional domains and motifs is attributed to the evolution of these groups from different ancestors, thus validating the accuracy of clusters in the phylogenetic tree. However, the intron-exon organization is not precisely similar, which suggests duplication of genes and complex alternative splicing. The percentage of secondary structural elements is comparable for members of the same group, but the tertiary conformation is varied and dominated by coiled-coil segments required for catalytic activity. Gene ontology analysis revealed that these proteins are mainly associated with the catalytic activity of ubiquitination, immune system, zinc ion binding, positive regulation of transcription, ligase activity, and cell cycle regulation. Moreover, the biological pathway analyses identified four KEGG and 22 Reactome pathways. The predicted pathways correspond to functional domains, and gene ontology which proposes that proteins with similar structures might perform the same functions.

Assessment of the Hydrological Conditions of Carps Spawning Grounds in the Sylhet Haor Basins, and the Halda River System, Bangladesh

The Halda River and Haor basins are considered unique breeding habitats for carps in Bangladesh. The purpose of the study was to evaluate the natural spawning environment of carp species, with an emphasis on the hydrological conditions of the Halda River, the Surma River, and Tanguar Haor. This study, which covers the years 2021 and 2022, considered two spawning seasons. The results revealed that temperature, total dissolved solids (TDS), salinity, turbidity, conductivity, and pH were significantly different in the Halda River to the Surma River and Tanguar Haor. However, sudden rainfall and upstream runoff in 2021 were deemed as major factors causing an increment in TDS and electrical conductivity. The water pollution index was excellent in the Surma River and Tanguar Haor, and good in the Halda River. The study reported moderately reduced IMC fry production in 2021 because of inconsistent turbidity, high conductivity, and increased salinity which interrupted the ideal spawning habitat. However, in 2022, the spawning environment was favorable in the Halda River, while the Surma River and Tanguar Haor were considered to be comparatively favorable in both years. Differences were also found in the climatic and meteorological conditions, which revealed uneven rainfall, higher temperatures, decreasing water discharge, and low water levels. The meteorological data also revealed that the overall rainfall showed a decreasing trend for all sites in the last 20 years causing an interrupted water discharge. It was discovered that the amount of carp spawn obtained in the Halda River was greater than that obtained in the Surma River and Tanguar Haor. In terms of hydrological conditions and spawning performance, the Surma River and Tanguar Haor were reported to have significant differences, which may be due to their geographical location.