Related Species Research Articles

Fuscoporia nebularis, (Hymenochaetaceae) a new endemic and threatened species from the cloud forests of southern Brazil

A new and threatened species of Fuscoporia from the cloud forests of southern Brazil is described. This species, associated with hardwood species of Myrtaceae, differs from other Fuscoporia species by having broadly ellipsoid basidiospores (2.9–3.8 × 2.3–3.1 µm) and hymenial setae that occasionally have a bifurcate and curved apex. Additionally, molecular analysis based on ITS and nrLSU sequences supports the new species. The conservation status of this new species of Fuscoporia is assessed as ‘Endangered’ based on IUCN criteria. We present the new species with illustrated morphological description, phylogenetic analyses and comparison with morphologically similar or related species. A key to the neotropical species of Fuscoporia is provided.

Genome-wide identification of the E-class gene family in wheat: evolution, expression, and interaction

IntroductionWheat (Triticum aestivum L.) is among themost important crop worldwide. Given a growing population and changing climate, enhancing wheat yield is of great importance. Yield is closely associated with flower and spike development, and E-class genes play important roles in the flower and kernel development of plants. Currently, the absence of systematic analysis on the E gene family hinders our comprehension of their roles in plant growth and development.MethodsIdentify E-class genes based on homologous sequence searches. Analyze the identified E-class genes through a series of gene family analyses. Determine the expression levels of wheat E-class genes by searching public databases. Validate the functions of these genes by transforming them into Arabidopsis. Finally, determine the interactions between the genes through yeast two-hybrid experiments.ResultsFifteen E-class genes (TaEs) were identified in common wheat. Nine E-class genes were detected in five ancestral/closely related species, including one in Aegilops tauschii (AtE), one in T. Urartu (TuEs), two in T. turgidum (TtEs), two in T. dicoccoides (TdEs), and three in T. spelta (TsEs). The 24 E-class genes were classified into three subgroups using a phylogenetic approach. All genes were highly expressed in spikes, and most were only highly expressed at the floret meristem stage. The effects of TaSEP5-A on flowering and growth cycles were confirmed in homologous mutants and transgenic Arabidopsis thaliana. The E-class genes were able to regulate the growth cycle of Arabidopsis. Finally, we confirmed the interactions between TaSEP5-A and other wheat E-class genes based on yeast two-hybrid assays.DiscussionOur findings provide information regarding the E-class genes in wheat and will potentially promote the application of these genes in wheat improvement.

Comparative plastome analyses and evolutionary relationships of 25 East Asian species within the medicinal plant genus Scrophularia (Scrophulariaceae)

BackgroudScrophularia L., a genus of the Scrophulariaceae, is a group of important medicinal plants used for eliminating heat and detoxifying. East Asia has an abundance of potentially medicinal Scrophularia species, and it serves as a secondary diversity center of the genus. However, the genomic resources available for germplasm identification and pharmaceutical exploration of East Asian Scrophularia are insufficient, hindering its commercial and industrial development. Additionally, the interspecific relationships of most East Asian Scrophularia species remain unclear.MethodsIn this study, we sequenced the leaves of 25 East Asian species of the genus Scrophularia, assembled and annotated the complete chloroplast genomes, and subsequently performed comparative and phylogenetic analyses on these genomes.Results and discussionThe conserved plastome length of these 25 species ranged from 151,582 bp to 153,239 bp, containing a total of 132 coding genes, including 18 duplicated genes and 114 unique genes. Through genome alignment of these 25 species, 38-53 repeated sequences and 7 shared SSRs were identified, along with regions with high nucleotide polymorphism (Pi), which could potentially serve as molecular markers for species identification. The genome structure, gene content, and arrangement showed conservation, while variations were observed in the IR boundary regions and IGS. Phylogenetic inferences based on whole plastomes or on coding sequences (CDS) only yielded congruent results. We categorized the 25 East Asian Scrophularia species into six distinct clades and further explored their interspecies relationships using morphological characteristics, such as flower color, the relative position of stamens and corolla, and plant height. This could lay a genetic basis for future resource development of Scrophularia in East Asia.

Geography and associated bioclimatic factors differentially affect leaf phenolics in three ivy species (Hedera L.) across the Iberian Peninsula

The biosynthesis of secondary metabolites in plants, especially that of phenolic compounds, is stimulated to protect against several environmental stress factors such as cold temperatures, drought, and UV-irradiance. As a result, when a species occurs under different climatic conditions, differences in phenolic accumulation are expected across species distribution in response to the environmental cues. However, our understanding of phenolic compounds' natural variation is limited, as most of our knowledge on secondary metabolite biosynthesis stems from experimental studies conducted under controlled conditions. In this study we analyze phenolic content and its relation to climatic and geographic variation in three closely related Hedera species (H. helix, H. hibernica and H. iberica) across their southwestern range limits in the Iberian Peninsula (82 populations, 401 individuals). The Iberian Peninsula concentrates the highest global species richness of Hedera, with the three species sharing range boundaries along the latitudinal and longitudinal climatic gradient of the region. We found that the three species exhibited different climatic and geographic patterns of phenolic content variation in the study area. The phenolic production in H. helix increased with elevation in relation to the decrease of temperature and the increase of temperature contrast, whereas in H. hibernica varies with latitude in relation to summer temperature and precipitation regimes, increasing in areas with no summer drought. In contrast, we did not find any environmental variables associated with phenolic content in H. iberica, likely due to its narrow geographic and climatic range and a higher influence of microclimatic conditions. Although the three Hedera species are closely related, our results suggest that leaf phenolic production may be triggered by different environmental conditions in each species. Our study underscores the species-specific nature of phenolic compounds' role in plant stress response.

Same-Sex Sexual Behavior, Age-Class, and Coalitions in Male Rhesus Macaques (Macaca mulatta).

In the present study, relations between same-sex sexual behavior (SSB), age-class, and coalitional behavior in male rhesus macaques were examined in a re-analysis of data first analyzed and reported by Clive et al. (2023). Age-class as a focal variable was indicated in an extensive literature review, which showed that male non-adult (juvenile, adolescent) participation in SSB is extensive in this and related primate species and associated with various benefits. Clive et al. (2023) excluded juveniles from their analysis. In the re-analysis (n = 995 mounting events), it was found that non-adult involvement was substantial (51%). Most dyads contained at least one non-adult (76%). Young and prime adult mounters most often selected non-adults to mount. Mounters were often sexually motivated: most for adolescents (72%); equally for juveniles (57%) and adults (56%). Finally, the highest rate of SSB with coalitional context appeared in adolescent-adult dyads involved in multiple repeated mounts. SSB, age-class, special friendships, bonding, and coalitions were linked, as reported in some other primate species and human societies cross-culturally. Employing age-class in male SSB analysis improved description and explanation.

Tip extension and simultaneous multiple fission in a filamentous bacterium

Organisms display an immense variety of shapes, sizes, and reproductive strategies. At microscopic scales, bacterial cell morphology and growth dynamics are adaptive traits that influence the spatial organization of microbial communities. In one such community-the human dental plaque biofilm-a network of filamentous Corynebacterium matruchotii cells forms the core of bacterial consortia known as hedgehogs, but the processes that generate these structures are unclear. Here, using live-cell time-lapse microscopy and fluorescent D-amino acids to track peptidoglycan biosynthesis, we report an extraordinary example of simultaneous multiple division within the domain Bacteria. We show that C. matruchotii cells elongate at one pole through tip extension, similar to the growth strategy of soil-dwelling Streptomyces bacteria. Filaments elongate rapidly, at rates more than five times greater than other closely related bacterial species. Following elongation, many septa form simultaneously, and each cell divides into 3 to 14 daughter cells, depending on the length of the mother filament. The daughter cells then nucleate outgrowth of new thinner vegetative filaments, generating the classic "whip handle" morphology of this taxon. Our results expand the known diversity of bacterial cell cycles and help explain how this filamentous bacterium can compete for space, access nutrients, and form important interspecies interactions within dental plaque.

Extensive transmission and variation in a functional receptor for praziquantel resistance in endemic Schistosoma mansoni.

Population genomics and functional genetics of praziquantel resistance in Schistosoma mansoni.

Placental Evolution: Innovating how to Feed Babies.

The evolution of the placenta was transformative. It changed how offspring are fed during gestation from depositing all the resources into an egg to continually supplying resources throughout gestation. Placental evolution is infinitely complex, with many moving parts, but at the core it is driven by a conflict over resources between the mother and the baby, which sets up a Red Queen race, fueling rapid diversification of morphological, cellular, and genetic forms. Placentas from even closely related species are highly divergent in form and function, and many cellular processes are distinct. If we could extract the entirety of genomic information for placentas across all species, including the many hundreds that have evolved in fish and reptiles, we could find their shared commonality, and that would tell us which of the many pieces really matter. We do not have this information, but we do have clues. Convergent evolution mechanisms were repeatedly used in the placenta, including the intense selective pressure to co-opt an envelope protein to build a multinucleated syncytium, the use of the same hormones and structural proteins in placentas derived from separate embryonic origins that arose hundreds of millions of years apart, and the co-option of endogenous retroviruses to form capsids as a way of transport and as mutagens to form new enhancers. As a result, the placental genome is the Wild West of biology, set up to rapidly change, adapt, and innovate. This ability to adapt facilitated the evolution of big babies with big brains and will continue to support offspring and their mothers in our ever-changing global environment.

Eplingiella sanoi sp. nov. (Hyptidinae-Lamiaceae): supports the urgent need for campos rupestres conservation in the Serra do Espinhaço Septentrional, Minas Gerais state, Brazil

A new species of Lamiaceae, Eplingiella sanoi, from the campo rupestre of an ecotone zone between the Cerrado and the Caatinga domains is described and illustrated. The new species expands the distribution of the genus to Southeast Brazil, previously restricted to Northeast Brazil. Eplingiella sanoi is compared to the three other species of the genus, especially E. cuniloides, the morphologically closest related species. We present an identification key for all the species of the genus, an occurrence map for Eplingiella sanoi, E. cuniloides and E. brightoniae and a formal conservation assessment for the new species. Eplingiella sanoi is endemic to the region between Pico da Formosa and Pico do Sucuruiú (Minas Gerais state, Serra do Espinhaço), an area under threat due to a planned wind farm installation. Recognizing this species emphasizes the imperative for more taxonomic studies and conservation of Serra do Espinhaço.

Morphological description and DNA barcoding of Thalassomya paraskevae sp. nov. (Diptera: Chironomidae: Telmatogetoninae) from coast of the Black Sea

Illustrated description of adult male, as well as DNA barcoding, of Thalassomya paraskevae sp. nov. in comparison with close related species T. frauenfeldi Schiner from coast of the Black Sea are provided. Interspecific p-distances using gene COI 5P between T. paraskevae sp. nov. and T. frauenfeldi from Madeira (Portugal) were 8.94%. Divergence between described species and T. frauenfeldi collected near the type locality (Friuli Venezia-Giulia, Italy) using COI 3P were 7.72%. Obtained values corresponds to species level.

The assembly and comparative analysis of the first complete mitogenome of Lindera aggregata

Lindera aggregata, a member belongs to the genus Lindera of Lauraceae family. Its roots and leaves have been used as traditional Chinese medicine or functional food for thousands of years. However, its mitochondrial genome has not been explored. Our aim is to sequence and assemble the mitogenome of L. aggregata to elucidate the genetic mechanism and evolutionary pathway. The results had shown that the mitogenome was extremely complex and had a unique multi-branched conformation with total size of 912,473 bp. Comprehensive analysis of protein coding genes of 7 related species showed that there were 40 common genes in their mitogenome. Interestingly, positive selection had become an important factor in the evolution of ccmB, ccmFC, rps10, rps11 and rps7 genes. Furthermore, our data highlighted the repeated trend of homologous fragment migrations between chloroplast and mitochondrial organelles, and 38 homologous fragments were identified. Phylogenetic analysis identified a tree that was basically consistent with the phylogeny of Laurales species described in the APG IV system. To sum up, this study will be helpful to the study of population genetics and evolution of Lindera species.

Contribution to the genus Clypeocaenis Soldán, 1978 (Ephemeroptera: Caenidae) from Lao People’s Democratic Republic with a key to Oriental species

Larvae of Clypeocaenis laosensis sp. nov. were collected from the Xe Nou River, Xe Bang Hieng River, and Tha Hao Stream in central and southern Lao PDR. This investigation also encompassed the examination of eggs extracted from female larvae, and we give here the first egg description of Cl. multisetosa Soldán, 1978 and Cl. oligosetosa Soldán, 1983. Clypeocaenis laosensis sp. nov. is closely related with Cl. gayathri Balasubramanian & Muthukatturaja, 2020 and Cl. kaveri Balasubramanian & Muthukatturaja, 2021. The new species is distinguishable from other Clypeocaenis species by specific morphological traits. These include the number of setae on the clypeus, the morphology of the labial palpus, the length of marginal setae on the middle and hind femora, setae of the transverse row on the forefemur, and body surface microtrichia. A detailed comparison of the morphological features between this new species and its closely related species is provided. Furthermore, the first-time reporting of Cl. multisetosa and Cl. oligosetosa in Lao PDR expands the known Caenidae diversity in the country to five species. A key to the nymphs of the Oriental species of Clypeocaenis is provided.

Interorgan, intraorgan and interplant communication mediated by nitric oxide and related species.

Plant survival to a potential plethora of diverse environmental insults is underpinned by coordinated communication amongst organs to help shape effective responses to these environmental challenges at the whole plant level. This interorgan communication is supported by a complex signal network that regulates growth, development and environmental responses. Nitric oxide (NO) has emerged as a key signalling molecule in plants. However, its potential role in interorgan communication has only recently started to come into view. Direct and indirect evidence has emerged supporting that NO and related species (S-nitrosoglutathione, nitro-linolenic acid) are mobile interorgan signals transmitting responses to stresses such as hypoxia and heat. Beyond their role as mobile signals, NO and related species are involved in mediating xylem development, thus contributing to efficient root-shoot communication. Moreover, NO and related species are regulators in intraorgan systemic defence responses aiming an effective, coordinated defence against pathogens. Beyond its in planta signalling role, NO and related species may act as ex planta signals coordinating external leaf-to-leaf, root-to-leaf but also plant-to-plant communication. Here, we discuss these exciting developments and emphasise how their manipulation may provide novel strategies for crop improvement.

Wolbachia strain diversity in a complex group of sympatric cryptic parasitoid wasp species

BackgroundMaternally-inherited symbionts can induce pre-mating and/or post-mating reproductive isolation between sympatric host lineages, and speciation, by modifying host reproductive phenotypes. The large parasitoid wasp genus Cotesia (Braconidae) includes a diversity of cryptic species, each specialized in parasitizing one to few related Lepidoptera host species. Here, we characterized the infection status of an assemblage of 21 Cotesia species from 15 countries by several microbial symbionts, as a first step toward investigating whether symbionts may provide a barrier to gene flow between these parasitoid host lineages.ResultsThe symbiotic microbes Arsenophonus, Cardinium, Microsporidium and Spiroplasma were not detected in the Cotesia wasps. However, the endosymbiotic bacterium Wolbachia was present in at least eight Cotesia species, and hence we concentrated on it upon screening additional DNA extracts and SRAs from NCBI. Some of the closely related Cotesia species carry similar Wolbachia strains, but most Wolbachia strains showed patterns of horizontal transfer between phylogenetically distant host lineages.ConclusionsThe lack of co-phylogenetic signal between Wolbachia and Cotesia suggests that the symbiont and hosts have not coevolved to an extent that would drive species divergence between the Cotesia host lineages. However, as the most common facultative symbiont of Cotesia species, Wolbachia may still function as a key-player in the biology of the parasitoid wasps. Its precise role in the evolution of this complex clade of cryptic species remains to be experimentally investigated.

Evidence for moth pollination in a rhinomyiophilous Erica species from the Cape Floristic Region of South Africa.

Contrasting pollination syndromes in closely related species suggest that floral trait divergence is associated with differences in pollination system, but empirical observations are required to confirm syndrome-based predictions. We present a comparative study of two closely related Erica species with contrasting pollination syndromes from the Cape Floristic Region of South Africa. Ericacylindrica has narrowly tubular pale and strongly scented flowers and is known to be hawkmoth-pollinated. The closely related Ericainfundibuliformis has bright flower colours and appears to lack scent, traits that are suggestive of pollination by long-tongued nemestrinid flies (rhinomyiophily). Floral trait measurements revealed that both species exhibit predominantly upright flower orientation and elongated floral tubes, although tube length of E.infundibuliformis is consistently greater than that of E.cylindrica. For both species, petals are brighter than floral tube surfaces, but flowers of E.cylindrica lack the strong UV reflectance found in E.infundibuliformis. Nectar of E.infundibuliformis is more concentrated and produced in larger volumes. Scent composition, but not evening scent emission rates, differed between the species: scent of E.cylindrica is dominated by aromatic compounds, whereas scent of E.infundibuliformis is dominated by (E)-ocimene and other terpenoid compounds and is emitted at higher rates during the day than the evening. Pollinator observations contradicted trait-based predictions: although a single nemestrinid fly captured in the vicinity of E.infundibuliformis did carry Erica pollen, almost all other diurnal flower visitors were nectar-robbing Hymenoptera which did not carry Erica pollen. Contrary to predictions, at two sites and over two flowering seasons, flowers were consistently visited in the evenings by several species of settling moths and hawkmoths which carried pollen, almost exclusively of Erica, on their proboscides. Our findings thus suggest that, despite objective differences in key floral traits between the closely related hawkmoth-pollinated E.cylindrica and E.infundibuliformis, moths are also important pollinators of E.infundibuliformis. A bimodal pollination system involving predominant pollination by moths and occasional visits by long-proboscid flies could partially reconcile findings with predictions. Our study further suggests that hawkmoth pollination may be more widespread in both Erica and the broader Cape flora than has hitherto been assumed and emphasises the importance of nocturnal pollinator observations.

The Paussus (Scaphipaussus) lanxangensis species group of Indomalayan ant nest beetles (Coleoptera, Carabidae, Paussinae, Paussini) with description of a new species

The new species Paussus agatae sp. nov. could not easily be allocated to an established subgenus or even species group. The informal Paussus lanxangensis species group is established to include the new species and other related species. Its diagnostic characters are described and screened for possible use in cladistic analyses in the future. Currently, it consists of five described species of the Indomalayan mainland, which are arranged in a key.

Phylogenomics analysis of Scutellaria (Lamiaceae) of the world

BackgroundScutellaria, a sub-cosmopolitan genus, stands as one of the Lamiaceae family’s largest genera, encompassing approximately 500 species found in both temperate and tropical montane regions. Recognized for its significant medicinal properties, this genus has garnered attention as a research focus, showcasing anti-cancer, anti-inflammatory, antioxidant, and hepatoprotective qualities. Additionally, it finds application in agriculture and horticulture. Comprehending Scutellaria’s taxonomy is pivotal for its effective utilization and conservation. However, the current taxonomic frameworks, primarily based on morphological characteristics, are inadequate. Despite several phylogenetic studies, the species relationships and delimitations remain ambiguous, leaving the genus without a stable and reliable classification system.ResultsThis study analyzed 234 complete chloroplast genomes, comprising 220 new and 14 previously published sequences across 206 species, subspecies, and varieties worldwide. Phylogenetic analysis was conducted using six data matrices through Maximum Likelihood and Bayesian Inference, resulting in a robustly supported phylogenetic framework for Scutellaria. We propose three subgenera, recommending the elevation of Section Anaspis to subgeneric rank and the merging of Sections Lupulinaria and Apeltanthus. The circumscription of Subgenus Apeltanthus and Section Perilomia needs to be reconsidered. Comparative analysis of chloroplast genomes highlighted the IR/SC boundary feature as a significant taxonomic indicator. We identified a total of 758 SSRs, 558 longer repetitive sequences, and ten highly variable regions, including trnK–rps16, trnC–petN, petN–psbM, accD–psaI, petA–psbJ, rpl32–trnL, ccsA–ndhD, rps15–ycf1, ndhF, and ycf1. These findings serve as valuable references for future research on species identification, phylogeny, and population genetics.ConclusionsThe phylogeny of Scutellaria, based on the most comprehensive sample collection to date and complete chloroplast genome analysis, has significantly enhanced our understanding of its infrageneric relationships. The extensive examination of chloroplast genome characteristics establishes a solid foundation for the future development and utilization of Scutellaria, an important medicinal plant globally.

Oreonectes yuedongensis (Cypriniformes, Nemacheilidae), a new freshwater fish species from the Lianhua Mountains in eastern Guangdong, China.

In this work, we describe a new species of the genus Oreonectes, Oreonectes yuedongensis sp. nov., collected from the Lianhua Mountains in eastern Guangdong, China. Phylogenetic trees constructed based on the mitochondrial cytochrome b (Cyt b) gene showed that this new species represents an independent evolutionary lineage, with uncorrected genetic distances (Kimura 2-parameter model) from congeners ranging from 5.1% to 8.3%. In addition, nuclear DNA analysis indicated O. yuedongensis as an independent lineage separate from its closely related species. Morphologically, the new species can be distinguished from other six species in the genus Oreonectes by a combination of serial characters. The description of this new species suggests that it is necessary to reassess the biodiversity of Oreonectes platycephalus as a complex, especially in the middle reaches of the Pearl River near the border between Guangdong and Guangxi. Morphological and genetic evidence supports O. yenlingi as a synonym of O. platycephalus.

Phylogenetic relatedness of plant species co‐occurring with an invasive alien plant species varies with elevation

AbstractDarwin's naturalization conundrum posits that the alien species either succeed in the introduced region because they are phylogenetically related to the native species and thus tend to have niches similar to those of native species, or they are phylogenetically dissimilar to native species and thus occupy unfilled niches. This conundrum has received a lot of attention, but the findings regarding these two hypotheses have been contradictory. To reconcile the two seemingly contradictory hypotheses, we assessed the phylogenetic relationship of a highly widespread and invasive species, Anthemis cotula L. (focal species), separately with the native species and all its co‐occurring species (including native and non‐native species) along an elevation gradient. Our results show that the abundance of A. cotula declined continuously with an increase in elevation and with an increase in the number of both all co‐occurring and native species only. The phylogenetic distance between the focal species and the native species based on weighted mean pairwise distance (MPDaw) declined with elevation as well as native species richness. Our study also revealed that soil nutrients strongly influence the abundance of A. cotula; however, their effect on the phylogenetic distance between the focal and other species is negligible. These findings suggest a strong role of microecological factors and spatial heterogeneity in the abundance distributions of invasive species and community assembly. Thus, it could be concluded that the phylogenetic relationship of A. cotula with co‐occurring all or only native species varies with elevation and species richness, adding another layer of complexity to the resolution of Darwin's naturalization conundrum.

Amazonian rivers are leaky barriers to gene flow in forest understory birds.

Ever since Alfred Russel Wallace's nineteenth-century observation that related terrestrial species are often separated on opposing riverbanks, major Amazonian rivers have been recognized as key drivers of speciation. However, rivers are dynamic entities whose widths and courses may vary through time. It thus remains unknown how effective rivers are at reducing gene flow and promoting speciation over long timescales. We fit demographic models to genomic sequences to reconstruct the history of gene flow in three pairs of avian taxa fully separated by different Amazonian rivers, and whose geographic ranges do not make contact in headwater regions where rivers may cease to be barriers. Models with gene flow were best fit but still supported an initial period without any gene flow, which ranged from 187 000 to over 959 000 years, suggesting that rivers are capable of initiating speciation through long stretches of allopatric divergence. Allopatry was followed by either bursts or prolonged episodes of gene flow that retarded genomic differentiation but did not fully homogenize populations. Our results support Amazonian rivers as key barriers that promoted speciation and the build-up of species richness, but they also suggest that river barriers are often leaky, with genomic divergence accumulating slowly owing to episodes of substantial gene flow.