The traditional villages in the Miao Frontier Corridor are the products of migrations, social interactions, and transportation, as well as production interchanges between the central plains and the frontiers of China in ancient times. They have made significant contributions to local multicultural inheritance and regional social development. However, with the increasing pressure of heritage conservation and sustainable development, there is growing attention on how traditional villages can tap into their cultural continuity and distinctiveness. This study introduces the concept of cultural routes, with the aim of integrating traditional villages of different ethnicities, regions, and characteristics from the perspective of diversity. It analyzes their spatial differentiation characteristics and the factors influencing them, providing basic support for the overall protection of traditional villages with special characteristics. Following this idea, 338 remaining traditional villages in the Miao Frontier Corridor were selected as the research objects. With the help of 91-satellite maps and a geographic information system (GIS), a cultural and geographic database of the traditional villages in the Miao Frontier Corridor was constructed to objectively explore the roles of the traditional villages’ natural geography, historical, and humanistic elements in the spatial categorization on a large scale. This study shows that the spatial distribution of the traditional villages in the Miao Frontier Corridor is uneven, exhibiting a cluster structure with of a “single primary nucleus with multiple secondary nuclei”. The spatial differentiation of traditional villages exhibits a similar clustering pattern based on individual natural geographic factors, such as elevation, mountain undulation, slope, and water systems. Additionally, there is discernible regularity concerning historical and humanistic factors, such as ethnicity type, age of village establishment, and the presence of guard stations. Further exploring the micro-spatial level, the natural geographical environment serves as the structural foundation of traditional village space, while the historical and humanistic environment fosters multiple differentiations in traditional village space in terms of influencing factors. Together, these factors jointly influence the spatial differentiation of traditional villages. This study enriches the dynamic aspects of linear cultural heritage preservation and also provides new insights into the specialized development within the overall protection of traditional villages.
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