At present time researchers investigate approaches to the automatic facial micro-expression analysis to implement the possibility of the recognition of true human emotions from the image sequence of his face. The modern technologies are based on the use of complex mathematical algorithms with many adjusted parameters; in particular, such algorithms are the algorithms of spatio-temporal feature descriptors. The scientists who are not technical specialists, e.g., psychologists or psychotherapists, when investigating these algorithms may encounter difficulties in writing the program code. Therefore, it became necessary to develop a special software package for the investigation of spatio-temporal feature descriptors. Aim of the work is development of a special software package for studying various spatial and temporal descriptors of facial micromovement features in video images A software package for the investigation of spatio-temporal feature descriptors with a graphical interface is developed. The software package makes it possible to investigate the influence of the selection of the descriptor (LBP-TOP, FHOOF or FHOFO) and its parameters on the micro-facial movement detection accuracy for different input datasets of human face image sequences. The developed software package for the investigation of spatio-temporal feature descriptors can be applied as a software tool for carrying out investigations in the facial micro-expression analysis field.