Spatiotemporal Adaptations Research Articles

Novice Users of a Bodyweight-Supporting Treadmill Require Familiarization.

Previous work has demonstrated an improvement in running economy during sustained running on a lower body positive pressure treadmill, but neuromuscular and spatiotemporal measures have only been investigated during short-duration running bouts on these devices. The current study sought to replicate the noted metabolic response and investigate whether neuromuscular and/or spatiotemporal adaptations underlie the noted improvements in running economy. Cross-sectional. Fifteen trained runners (11 males and 4 females) ran three 15-minute trials with 30% bodyweight support at 70% of the speed that elicited their peak oxygen consumption while running on a standard treadmill. A series of 1-way analyses of variance with repeated measures were used to explore differences in dependent variables over the 45minutes of running. Dependent variables included oxygen consumption, root-mean-square electromyography of the vastus medialis and medial gastrocnemius during stance, and spatiotemporal parameters. Oxygen consumption decreased after the initial exposure, with no further reductions after 20minutes. Root-mean-square electromyography of the vastus medialis and medial gastrocnemius also decreased over time, with no further reductions after 20 and 10minutes, respectively. No differences in spatiotemporal parameters were found. Future research should provide sufficient time for runners to develop a more economical gait pattern prior to collecting dependent variables, and previous findings using lower body positive pressure treadmills may need to be reconsidered. Athletes using these devices for training or rehabilitation should note that increased economy will lower the intensity of a given treadmill setting over time.

Spatiotemporal Adaptations of Macrophage and Dendritic Cell Development and Function.

Macrophages and conventional dendritic cells (cDCs) are distributed throughout the body, maintaining tissue homeostasis and tolerance to self and orchestrating innate and adaptive immunity against infection and cancer. As they complement each other, it is important to understand how they cooperate and the mechanisms that integrate their functions. Both are exposed to commensal microbes, pathogens, and other environmental challenges that differ widely among anatomical locations and over time. To adjust to these varying conditions, macrophages and cDCs acquire spatiotemporal adaptations (STAs) at different stages of their life cycle that determine how they respond to infection. The STAs acquired in response to previous infections can result in increased responsiveness to infection, termed training, or in reduced responses, termed paralysis, which in extreme cases can cause immunosuppression. Understanding the developmental stage and location where macrophages and cDCs acquire their STAs, and the molecular and cellular players involved in their induction, may afford opportunities to harness their beneficial outcomes and avoid or reverse their deleterious effects. Here we review our current understanding of macrophage and cDC development, life cycle, function, and STA acquisition before, during, and after infection.We propose a unified framework to explain how these two cell types adjust their activities to changing conditions over space and time to coordinate their immunosurveillance functions.

Increased Arm Swing and Rocky Surfaces Reduces Postural Control in Healthy Young Adults.

Fall-induced injuries can stem from a disruption in the postural control system and place a financial burden on the healthcare system. Most gait research focused on lower extremities and neglected the contribution of arm swing, which have been shown to affect the movement of the center of mass when walking. This study evaluated the effect of arm swing on postural control and stability during regular and rocky surface walking. Fifteen healthy young adults (age = 23.4 ± 2.8) walked on these two surfaces with three arm motions (normal, held, and active) using the CAREN Extended-System (Motek Medical, Amsterdam, NL). Mean, standard deviation and maximal values of trunk linear and angular velocity were calculated in all three axes. Moreover, step length, time and width mean and coefficient of variation as well as margin of stability mean and standard deviation were calculated. Active arm swing increased trunk linear and angular velocity variability and peak values compared to normal and held arm conditions. Active arm swing also increased participants’ step length and step time, as well as the variability of margin of stability. Similarly, rocky surface walking increased trunk kinematics variability and peak values compared to regular surface walking. Furthermore, rocky surface increased the average step width while reducing the average step time. Though this surface type increased the coefficient of variation of all spatiotemporal parameters, rocky surface also led to increased margin of stability mean and variation. The spatiotemporal adaptations showed the use of “cautious” gait to mitigate the destabilizing effects of both the active arm swing and rocky surface walking and, ultimately, maintain dynamic stability.

Mitochondria in Embryogenesis: An Organellogenesis Perspective.

Organogenesis is well characterized in vertebrates. However, the anatomical and functional development of intracellular compartments during this phase of development remains unknown. Taking an organellogenesis point of view, we characterize the spatiotemporal adaptations of the mitochondrial network during zebrafish embryogenesis. Using state of the art microscopy approaches, we find that mitochondrial network follows three distinct distribution patterns during embryonic development. Despite of this constant morphological change of the mitochondrial network, electron transport chain supercomplexes occur at early stages of embryonic development and conserve a stable organization throughout development. The remodeling of the mitochondrial network and the conservation of its structural components go hand-in-hand with somite maturation; for example, genetic disruption of myoblast fusion impairs mitochondrial network maturation. Reciprocally, mitochondria quality represents a key factor to determine embryonic progression. Alteration of mitochondrial polarization and electron transport chain halts embryonic development in a reversible manner suggesting developmental checkpoints that depend on mitochondrial integrity. Our findings establish the subtle dialogue and co-dependence between organogenesis and mitochondria in early vertebrate development. They also suggest the importance of adopting subcellular perspectives to understand organelle-organ communications during embryogenesis.

How does the slope gradient affect spatiotemporal parameters during running? Influence of athletic level and vertical and leg stiffness

BackgroundThe current evidence leaves certain questions unanswered, including whether well-trained athletes adapt to different slope gradients in the same way as amateurs, and whether stiffness influences spatiotemporal adaptations during uphill running. Research questionThis study aimed to determine the effect of different slope gradients (0%–11%) on spatiotemporal gait characteristics during running, taking into account the influence of athletic level, vertical and leg stiffness. MethodsMale endurance runners (12 amateurs, 10 highly-trained) performed a running test on a motorized treadmill. The running velocity was set at 12 km/h, and participants completed six different running conditions (0, 3, 5, 7, 9 and 11% gradients). Spatiotemporal parameters were measured using the OptoGait system. Vertical (Kvert) and leg (Kleg) stiffness were calculated according to the sine-wave method. ResultsA 2 (amateur; highly-trained) × 6 (running conditions) ANOVA found no significant between-group differences in spatiotemporal parameters at any gradient (P ≥ 0.05); however, significant Kvert and Kleg differences (P < 0.05) were found within both groups with increasing gradients. Stepwise linear regression analysis showed that Kleg was strongly associated with contact time (R2 = 0.797, P < 0.001), whereas Kvert was associated with spatiotemporal adaptations to different slope gradients (R2 = 0.547, P = 0.002). SignificanceAn increased slope gradient (0–11%) at a given running velocity (12 km.h−1) caused spatiotemporal adaptations (i.e., increased CT and SF and decreased FT, SL and SA) regardless of the athletic level of the runner, although a non-significant trend differentiated the adaptations between the amateur and highly-trained groups. The results also indicated that leg stiffness plays a key role in the characteristics of spatiotemporal gait during level running, whereas vertical stiffness is strongly associated with spatiotemporal adaptations when running uphill.

Lack of Influence of Muscular Performance Parameters on Spatiotemporal Adaptations With Increased Running Velocity.

Roche-Seruendo, LE, García-Pinillos, F, Haicaguerre, J, Bataller-Cervero, AV, Soto-Hermoso, VM, and Latorre-Román, PÁ. Lack of influence of muscular performance parameters on spatiotemporal adaptations with increased running velocity. J Strength Cond Res 32(2): 409-415, 2018-This study aimed to analyze the influence of muscular performance parameters on spatiotemporal gait characteristics during running when gradually increasing speed. Fifty-one recreationally trained male endurance runners (age, 28 ± 8 years) voluntarily participated in this study. Subjects performed a battery of jumping tests (squat jump, countermovement jump, and 20-cm drop jump), and after that, the subjects performed an incremental running test (10-20 km·h) on a motorized treadmill. Spatiotemporal parameters were measured using the OptoGait system. Cluster k-means analysis grouped subjects according to the jumping test performance, by obtaining a group of good jumpers (n = 19) and a group of bad jumpers (n = 32). With increased running velocity, contact time was shorter and flight time and step length were longer, whereas cadence and stride angle were greater (p < 0.001). No significant differences between groups (p ≥ 0.05) were found at any running speed. The results obtained indicate that increased running velocity produced no differences in spatiotemporal adaptations between those runners with good jumping ability and those with poor jumping ability. Based on that, it seems that muscular performance parameters do not play a key role in spatiotemporal adaptations experienced by recreational endurance runners with increased velocity. However, taken into consideration the well-known relationship between running performance and neuromuscular performance, the authors suggest that muscular performance parameters would be much more determinant in the presence of fatigue (exhausted condition) or in the case of considering other variables such as running economy or kinetic.

Tooth movement mechanobiology

The current concept of “pressure-tension” tooth movement is based upon reductionist explanations of parsed physiological processes that do not provide a cohesive understanding of clinically relevant tooth movement. The biology underlying accelerated orthodontic techniques have refocused attention on disruptions of tensional integrity of microenvironments, resulting in epigenetic changes in cell products, and the intentional spatio-temporal adaptations of tissues to build strength to overcome tissue strain, all for the sake of re-establishing homeostasis. Differentiating how interstitial bone fluid pressure and flow is affected by the various tissue microporosities in regulating bone modeling and remodeling processes, will lead to a better understanding of tooth movement mechanobiology.

Effects of a dynamic-ankle-foot orthosis (Liberté®) on kinematics and electromyographic activity during gait in hemiplegic patients with spastic foot equinus.

A dynamic-ankle-foot orthosis has recently emerged and consists of an elastic band allowing the variation of stiffness degree and adjusts dorsiflexion assistance in swing. The aim of this study was to quantify the biomechanical adaptations induced by this orthosis during gait in hemiplegic patients. Twelve hemiplegic patients performed two gait analyses (without and with the ankle-foot orthosis). Spatiotemporal, kinematic, kinetic and electromyographic gait parameters were quantified using an instrumented gait analysis system during the stance and swing phases. During swing, peak ankle dorsiflexion was greater with the orthosis and associated with a decrease of pelvic obliquity angle. In stance, peak ankle plantarflexion and dorsiflexion were greater with the orthosis and associated with an increase of ankle angle at heel strike and toe-off. Electromyographic activities of both the tibialis anterior and the medial gastrocnemius were greater with the orthosis. This dynamic-ankle-foot orthosis improved gait in hemiplegic patients with spastic foot equinus. The spatiotemporal adaptations seem to be caused mainly by the increase of ankle dorsiflexion during stance and swing phases. The changes in electromyographic activity were related to an active dorsiflexion in stance and swing phases and an active plantarflexion in stance phase.

Age-related differences when walking downhill on different sloped terrains

Despite the common situation of walking on different sloped terrains, previous work on gait has focused on level terrain. This study aims to assess whether any age-related differences exist in spatiotemporal and stability parameters when walking downhill on three different sloped walkways. Two tri-axial accelerometers were used at the levels of head and pelvis to investigate spatiotemporal parameters, magnitude (root mean square, RMS), harmonic content of accelerations (harmonic ratios, HR) and attenuation between body levels (ATT) in 35 older adults (OA, 69±4.5y.o.) and 22 young adults (YA, 22.1±1.9y.o.). Older adults walked at the same speed and cadence as young adults in flat terrain (FL, 0%) and moderate hill (MH, 8%). In the highest slope (PH, 20%), older adults reduced speed and step length and both groups increased cadence. Age had no effect on attenuation and RMS profiles. RMS increased with slope in all directions at both head and pelvis, except, for medio-lateral direction (ML), with similar head RMS in all slopes. There is an important shift in ATT from anteroposterior direction (AP) to ML at the highest slope, resulting in smaller antero-posterior attenuation and greater medio-lateral attenuation. Age differences appeared in the smoothness (HR) at the flat terrain, with increased vertical and antero-posterior values for young adults. As slope increased, group differences disappeared and HR decreased for all directions of motion. In general, spatiotemporal adaptations to increased slope seem to be part of a mechanism to improve ML attenuation, in both young and old adults.

Inter-limb coordination and control in boys with and without DCD in ball catching

The purpose of the study was to examine the nature of inter-limb coordination and control, exhibited by ten boys with (M=10.5years, SD=1.0) and without Developmental Coordination Disorder (DCD), in a ball catching task across different ball speeds. In line with the predictions of coordinated structure theory boys who were typically developing preserved strong and effective temporal relations when catching balls in baseline and fast conditions. Boys with DCD exhibited temporal symmetry in baseline but not in fast attempts, where the decoupling was uniformed across the group, coinciding with failures on ≥50% of the attempts. In terms of stability, no differences in intra-individual variability were observed between the groups. The analysis of spatial and temporal adaptations showed that boys who were typically developing, in essence, covered the same “ground” by decreasing their movement time. The opposite was true for boys with DCD who contacted the ball closer to the body, while failing to adapt the velocity of their hands. Taken together, both groups exhibited strong and stable coordinative relations, in a steady-state but not in a fast condition, where all boys with DCD failed to preserve the desired symmetry. This decoupling also coincided with different and less effective spatio-temporal adaptations at the effector level. Thus, it appears that movement control, hence flexibility of their synergistic relations, represents a movement limiter for boys with DCD who exhibit specific balls skills issues, at inter-limb level of organization. It should be noted, however, that large intra-group variance observed across some variables warrants caution when interpreting the aggregate data.

Effects of a knee–ankle–foot orthosis on gait biomechanical characteristics of paretic and non-paretic limbs in hemiplegic patients with genu recurvatum

BackgroundA knee–ankle–foot orthosis may be prescribed for the prevention of genu recurvatum during the stance phase of gait. It allows also to limit abnormal plantarflexion during swing phase. The aim is to improve gait in hemiplegic patients and to prevent articular degeneration of the knee. However, the effects of knee–ankle–foot orthosis on both the paretic and non-paretic limbs during gait have not been evaluated. The aim of this study was to quantify biomechanical adaptations induced by wearing a knee–ankle–foot orthosis, on the paretic and non-paretic limbs of hemiplegic patients during gait. MethodsEleven hemiplegic patients with genu recurvatum performed two gait analyses (without and with the knee–ankle–foot orthosis). Spatio-temporal, kinematic and kinetic gait parameters of both lower limbs were quantified using an instrumented gait analysis system during the stance and swing phases of the gait cycle. FindingsThe knee–ankle–foot orthosis improved spatio-temporal gait parameters. During stance phase on the paretic side, knee hyperextension was reduced and ankle plantarflexion and hip flexion were increased. During swing phase, ankle dorsiflexion increased in the paretic limb and knee extension increased in the non-paretic limb. The paretic limb knee flexion moment also decreased. InterpretationWearing a knee–ankle–foot orthosis improved gait parameters in hemiplegic patients with genu recurvatum. It increased gait velocity, by improving cadence, stride length and non-paretic step length. These spatiotemporal adaptations seem mainly due to the decrease in knee hyperextension during stance phase and to the increase in paretic limb ankle dorsiflexion during both phases of the gait cycle.

The control and coordination of one-handed catching: the effect of temporal constraints.

The aim of the experiment was to identify the control mechanisms involved in a goal-directed task by manipulating the temporal constraints. Subjects were required to catch, with one hand, table tennis balls projected by a ball-projection machine under five temporal conditions (ball speed ranged from 5.7 to 9 m/s, giving rise to flight times of 550-350 ms). By means of three-dimensional kinematics analysis the following results were obtained, which are some spatio-temporal adaptations of the catching movement to the increase of temporal constraints: (1) a decrease in movement time, (2) an increase in the straightness of the trajectory of the wrist, and (3) a shift backwards of the place of ball-hand contact while (4) the grasping time was kept constant. Moreover, as a result of increasing temporal constraints, the acceleration phase of the transportation component was kept constant while the deceleration phase was progressively decreased and nearly suppressed. Further, an increased correlation of the initial direction of the transportation component with the initial direction of the ball path was found. These findings show some spatio-temporal adaptations of the catching movement when the time available is manipulated. They also support the contention of a shift in the control mechanisms involved in one-handed catching facing different temporal constraints.