It is of great practical significance to regional ecological conservation and restoration to explore the spatiotemporal variation characteristics of habitat quality in the ecologically fragile Loess Plateau. This study firstly explored the habitat quality in the Loess Plateau during 2000-2020 with the Integrated Valuation of Ecosystem Services and Trade-offs model. Then this study revealed the response characteristics of habitat quality to the fractional vegetation cover (FVC) change and human disturbance with the geographically weighted regression (GWR) model. Results showed habitat quality tended to improve in 51.16% of the study area, and area of high or very high habitat quality increased by 1.78%. Besides, FVC showed dominantly significant increase (62.42%) and high stability (69.66%) in the study area, and human disturbance increased remarkably in 18.11% of the study area but maintained the same level in 91.83% of the study area. Additionally, areas with positive correlation between habitat quality change and FVC and between habitat quality change and human disturbance change accounted for 52.56% and 37.38% of the study area, respectively, indicating FVC played dominant role in affecting the regional habitat quality variation. This study can provide important decision support information for the future ecological conservation of the Loess Plateau.