Spatial Resolution Scanning Research Articles

18F]fluorothymidine and [18F]fluorodeoxyglucose PET Imaging Demonstrates Uptake and Differentiates Growth in Neurofibromatosis 2 Related Vestibular Schwannoma.

To investigate whether [F]fluorothymidine (FLT) and/or [F]fluorodeoxyglucose (FDG) positron emission tomography (PET) can differentiate growth in neurofibromatosis 2 (NF2) related vestibular schwannomas (VS) and to evaluate the importance of PET scanner spatial resolution on measured tumor uptake. Six NF2 patients with 11 VS (4 rapidly growing, 7 indolent), were scanned with FLT and FDG using a high-resolution research tomograph (HRRT, Siemens) and a Siemens Biograph TrueV PET-CT, with and without resolution modeling image reconstruction. Mean, maximum, and peak standardised uptake values (SUV) for each tumor were derived and the intertumor correlation between FDG and FLT uptake was compared. The ability of FDG and FLT SUV values to discriminate between rapidly growing and slow growing (indolent) tumors was assessed using receiver operator characteristic (ROC) analysis. Tumor uptake was seen with both tracers, using both scanners, with and without resolution modeling. FDG and FLT uptake was correlated (R = 0.67-0.86, p < 0.01) and rapidly growing tumors displayed significantly higher uptake (SUVmean and SUVpeak) of both tracers (p < 0.05, one tailed t test). All of the PET analyses performed demonstrated better discriminatory power (AUCROC range = 0.71-0.86) than tumor size alone (AUCROC = 0.61). The use of standard resolution scanner with standard reconstruction did not result in a notable deterioration of discrimination accuracy. NF2 related VS demonstrate uptake of both FLT and FDG, which is significantly increased in rapidly growing tumors. A short static FDG PET scan with standard clinical resolution and reconstruction can provide relevant information on tumor growth to aid clinical decision making.

Super-Resolution Ultrasound Imaging by Sparse Bayesian Learning Method

Super-resolution ultrasound (SR-US) imaging technique overcomes the acoustic diffraction limit and greatly improves the spatial resolution. Furthermore, by exploiting temporal fluctuations in microbubbles, a super-resolution fluctuation imaging (SOFI) method has also been used to improve the temporal resolution of SR-US. However, this is at the expense of the reduction of spatial resolution. To address this problem, in this paper, a temporally correlated multiple sparse Bayesian learning (TMSBL) method is introduced to SR-US. Since TMSBL takes advantage of both the temporal correlation between successive frames and sparsity priors of microbubbles, it provides the possibility to obtain an enhancement in spatial resolution compared to SOFI (only considering the temporal fluctuations). To evaluate the performance of the proposed method, two types of numerical simulation and one physical phantom experiment were performed. Especially, we also investigate the effect of US imaging modes on the performance of SR-US implemented by TMSBL, where US data were acquired from the plane wave (the high imaging speed and the low spatial resolution) and synthetic transmit aperture (the low imaging speed and the high spatial resolution) scan, respectively. The experimental results show that compared to SOFI method when using the proposed TMSBL method, the imaging spatial resolution of SR-US can be improved. In addition, the imaging performance obtained by TMSBL can be hardly affected by US imaging modes. As a result, by combining with plane wave scan, TMSBL provides the potential in enhancing the spatial resolution of SR-US while maintaining a desired temporal resolution.

Insights into graphene wettability transparency by locally probing its surface free energy.

In this work, we study the surface energy of monolayer, bilayer and multilayer graphene coatings, produced through exfoliation of natural graphite flakes and chemical vapor deposition. We employ bimodal atomic force microscopy and micro-Raman spectroscopy for high spatial resolution and large area scanning of force of adhesion on the regions of the graphene/SiO2 surface with different graphene layers. Our measurements show that the interface conditions between graphene and SiO2 dominate the experimentally observed graphene surface energy. This finding sheds new light on the controversy surrounding graphene transparency studies. By separating the surface energy into polar and non-polar interactions, our findings suggest that monolayer graphene is nearly van der Waals opaque but partially transparent (near 60%) to polar interactions, which is further supported by characterizing graphene on the copper surface and two levels of density functional theory simulation. In addition to providing quantitative insight into the surface interactions of complicated graphene coatings, this work demonstrates a new route to nondestructively monitor the interface between graphene and coated substrates.

Diffraction limited mid-infrared reflectance microspectroscopy with a supercontinuum laser.

Chemical mapping was demonstrated with a mid-infrared (MIR) microspectroscopy setup based on a supercontinuum source (SC) emitting in the spectral range from 1.55 to 4.5 µm and a MEMS-based Fabry-Pérot filter spectrometer. Diffraction limited spatial resolution in reflection geometry was achieved. A multilayer film consisting of different polymers and mixtures thereof was measured and results were compared to those gained with a conventional FTIR microscope equipped with a thermal MIR source. Results show that compared to thermal sources, the application of the SC source results in higher signal-to-noise ratios together with better spatial resolution and faster scanning. Furthermore, diffraction limited imaging of red blood cells was demonstrated for the first time in the MIR spectral region in reflection mode. The distinctive characteristics of the MIR spectral region in conjunction with the high brightness, spatial coherence and broadband nature of supercontinuum radiation show the potential for improving infrared microscopy significantly.

Visualization and Quantification of Radiochemical Purity by Cerenkov Luminescence Imaging.

Determination of radiochemical purity is essential for characterization of all radioactive compounds, including clinical radiopharmaceuticals. Radio-thin layer chromatography (radio-TLC) has been used as the gold standard for measurement of radiochemical purity; however, this method has several limitations in terms of sensitivity, spatial resolution, two-dimensional scanning, and quantification accuracy. Here, we report a new analytical technique for determination of radiochemical purity based on Cerenkov luminescence imaging (CLI), whereby entire TLC plates are visualized by detection of Cerenkov radiation. Sixteen routinely used TLC plates were tested in combination with three different radioisotopes (131I, 124I, and 32P). All TLC plates doped with a fluorescent indicator showed excellent detection sensitivity with scanning times of less than 1 min. The new CLI method was superior to the traditional radio-TLC scanning method in terms of sensitivity, scanning time, spatial resolution, and two-dimensional scanning. The CLI method also showed better quantification features across a wider range of radioactivity values compared with radio-TLC and classical zonal analysis, especially for β--emitters such as 131I and 32P.

Encoding and readout strategies in magnetic resonance elastography.

Magnetic resonance elastography (MRE) has evolved significantly since its inception. Advances in motion-encoding gradient design and readout strategies have led to improved encoding and signal-to-noise ratio (SNR) efficiencies, which in turn allow for higher spatial resolution, increased coverage, and/or shorter scan times. The purpose of this review is to summarize MRE wave-encoding and readout approaches in a unified mathematical framework to allow for a comparative assessment of encoding and SNR efficiency of the various methods available. Besides standard full- and fractional-wave-encoding approaches, advanced techniques including flow compensation, sample interval modulation and multi-shot encoding are considered. Signal readout using fast k-space trajectories, reduced field of view, multi-slice, and undersampling techniques are summarized and put into perspective. The review is concluded with a foray into displacement and diffusion encoding as alternative and/or complementary techniques.

Fatigue crack initiation and growth in 43Fe-25Ni-22.5Cr austenitic steel at a temperature of 700 °C

Crack initiation and short crack growth in highly-alloyed austenitic steel Sanicro 25 cyclically strained at a temperature of 700 °C has been studied. Specimens cycled to 10% of the fatigue life and to the fracture were inspected using scanning electron microscopy and electron backscatter diffraction. On the surface, thin lamellae were extracted site-specifically by focused ion beam and then they were further characterized by high spatial resolution scanning transmission electron microscopy and energy dispersive X-ray spectroscopy. The preferential oxidation of grain boundaries was found to play a principal role in the initiation of multiple cracks. The oxide scale was analysed to show the distribution of individual elements during crack initiation and growth. Two types of crack paths were observed: intergranular due to the oxidation and transgranular accompanied by a cyclic plastic zone. Cracks preferentially initiated at the grain boundaries perpendicular to the stress axis and propagated in the bulk of the material. The role of the oxidation in high temperature fatigue crack initiation is discussed as well as the mechanisms of the crack initiation and growth.

High spatial resolution scanning for experimental room-acoustic measurements in scale models

This paper presents an experimental study of low frequency behavior of sound propagation in acoustically coupled volumes. The experiments are carried out in an eighth-scale model of two coupled volumes with an automated high spatial-resolution scanning system. Both sound pressure and three-dimensional sound intensities are automatically measured in terms of room impulse responses at each scanning positions over densely defined two-dimensional grids covering the entire areas of the two coupled rooms that enable the analysis and visualization of sound energy and sound energy flows. Through these measurements, and with the aid of a predictive computational model, this paper will discuss low frequency modal behaviors of sound energy flows within one of two coupled volumes. As such, the distribution of potential and kinetic energies, as well as that of active and reactive sound intensities within the coupled space will be separately analyzed. The distributions of intensity vector fields are compared through computational simulation. Consequently, a discussion on possible discrepancies in modal distributions between the computational and experimental results rounds off this paper.

A mixed-effects, spatially varying coefficients model with application to multi-resolution functional magnetic resonance imaging data.

Spatial resolution plays an important role in functional magnetic resonance imaging studies as the signal-to-noise ratio increases linearly with voxel volume. In scientific studies, where functional magnetic resonance imaging is widely used, the standard spatial resolution typically used is relatively low which ensures a relatively high signal-to-noise ratio. However, for pre-surgical functional magnetic resonance imaging analysis, where spatial accuracy is paramount, high-resolution functional magnetic resonance imaging may play an important role with its greater spatial resolution. High spatial resolution comes at the cost of a smaller signal-to-noise ratio. This begs the question as to whether we can leverage the higher signal-to-noise ratio of a standard functional magnetic resonance imaging study with the greater spatial accuracy of a high-resolution functional magnetic resonance imaging study in a pre-operative patient. To answer this question, we propose to regress the statistic image from a high resolution scan onto the statistic image obtained from a standard resolution scan using a mixed-effects model with spatially varying coefficients. We evaluate our model via simulation studies and we compare its performance with a recently proposed model that operates at a single spatial resolution. We apply and compare the two models on data from a patient awaiting tumor resection. Both simulation study results and the real data analysis demonstrate that our newly proposed model indeed leverages the larger signal-to-noise ratio of the standard spatial resolution scan while maintaining the advantages of the high spatial resolution scan.

Оценка и структурный анализ наноматериалов на сканирующем электронном микроскопе с высоким пространственным разрешением

Оценка и структурный анализ наноматериалов на сканирующем электронном микроскопе с высоким пространственным разрешением

Оценка и структурный анализ наноматериалов на сканирующем электронном микроскопе с высоким пространственным разрешением. Часть 2

Оценка и структурный анализ наноматериалов на сканирующем электронном микроскопе с высоким пространственным разрешением. Часть 2

Development of an Ultra High Resolution PET Scanner for Imaging Rodent Paws: PawPET.

A positron emission tomography (PET) scanner with submillimeter spatial resolution, capable of in vivo imaging of murine extremities was built based on two dual ended readout, hybrid depth of interaction (DOI) PET detectors. Each was composed of a 36 × 36 array of 0.43 mm × 0.43 mm × 8 mm unpolished lutetium oxyorthosilicate crystals separated by a 50 μm white diffuse reflector. The array was coupled to a position-sensing photomultiplier tube at one end and to an avalanche photodiode at the other end. The detector characterization included crystal identification accuracy, DOI, energy, and timing resolution measurements. The scanner was characterized in terms of its spatial resolution and its sensitivity and mouse images were acquired of a mouse paw injected with 18-F-NaF. Out of the 36 × 36 crystals only 33 × 33 crystals were identified. The coincidence timing, DOI, and energy resolution of the scanner was measured to be 2.8 ns, 1.4 mm, and 27%, respectively. The scanner's spatial resolution was measured with a line source and determined from an ordered subsets expectation maximization reconstruction to be 0.56 mm. The sensitivity of the scanner was measured to be 0.6% at the center of the field of view.

Generation of realistic virtual nodules based on three-dimensional spatial resolution in lung computed tomography: A pilot phantom study.

The aim of this feasibility study using phantoms was to propose a novel method for obtaining computer-generated realistic virtual nodules in lung computed tomography (CT). In the proposed methodology, pulmonary nodule images obtained with a CT scanner are deconvolved with the point spread function (PSF) in the scan plane and slice sensitivity profile (SSP) measured for the scanner; the resultant images are referred to as nodule-like object functions. Next, by convolving the nodule-like object function with the PSF and SSP of another (target) scanner, the virtual nodule can be generated so that it has the characteristics of the spatial resolution of the target scanner. To validate the methodology, the authors applied physical nodules of 5-, 7- and 10-mm-diameter (uniform spheres) included in a commercial CT test phantom. The nodule-like object functions were calculated from the sphere images obtained with two scanners (Scanner A and Scanner B); these functions were referred to as nodule-like object functions A and B, respectively. From these, virtual nodules were generated based on the spatial resolution of another scanner (Scanner C). By investigating the agreement of the virtual nodules generated from the nodule-like object functions A and B, the equivalence of the nodule-like object functions obtained from different scanners could be assessed. In addition, these virtual nodules were compared with the real (true) sphere images obtained with Scanner C. As a practical validation, five types of laboratory-made physical nodules with various complicated shapes and heterogeneous densities, similar to real lesions, were used. The nodule-like object functions were calculated from the images of these laboratory-made nodules obtained with Scanner A. From them, virtual nodules were generated based on the spatial resolution of Scanner C and compared with the real images of laboratory-made nodules obtained with Scanner C. Good agreement of the virtual nodules generated from the nodule-like object functions A and B of the phantom spheres was found, suggesting the validity of the nodule-like object functions. The virtual nodules generated from the nodule-like object function A of the phantom spheres were similar to the real images obtained with Scanner C; the root mean square errors (RMSEs) between them were 10.8, 11.1, and 12.5 Hounsfield units (HU) for 5-, 7-, and 10-mm-diameter spheres, respectively. The equivalent results (RMSEs) using the nodule-like object function B were 15.9, 16.8, and 16.5 HU, respectively. These RMSEs were small considering the high contrast between the sphere density and background density (approximately 674 HU). The virtual nodules generated from the nodule-like object functions of the five laboratory-made nodules were similar to the real images obtained with Scanner C; the RMSEs between them ranged from 6.2 to 8.6 HU in five cases. The nodule-like object functions calculated from real nodule images would be effective to generate realistic virtual nodules. The proposed method would be feasible for generating virtual nodules that have the characteristics of the spatial resolution of the CT system used in each institution, allowing for site-specific nodule generation.

Fast in situ 3D nanoimaging: a new tool for dynamic characterization in materials science

The performance of many advanced materials is determined by the arrangement of their nanostructure which requires ever more precise characterization. In this respect, X-ray computed tomography (CT) is a powerful technique to investigate material properties as it provides non-destructive direct access to three-dimensional morphology with nanoscale resolution. However, challenges remain in clearly understanding physical mechanisms involved during their processing in real time and real conditions. So far, beam and sample stabilities, effective spatial resolution and tomography scan time have hindered the development of nanoscale in situ 4D imaging (3D plus time), and especially at high temperatures. Here, we report on the development of fast X-ray nanotomography at temperatures up to 700°C with an unprecedented combination of nanometer pixel size and acquisition times of a few tens of seconds. The great potential of the method is demonstrated by following the early stages of two thermally driven phenomena: neck curvature evolution in sintering and nucleation of liquid droplets in light alloys. The reported real time observations will benefit the fundamental understanding of the underlying physics and provide useful data to build new models. The novel aspects of this synchrotron based technique offer a powerful imaging tool for a wide variety of heterogeneous nanoscale dynamics in materials and open new perspectives for the investigation of advanced materials under realistic conditions.

Using transmission Kikuchi diffraction to characterise α variants in an α+β titanium alloy.

SummaryTwo phase titanium alloys are important for high‐performance engineering components, such as aeroengine discs. The microstructures of these alloys are tailored during thermomechanical processing to precisely control phase fractions, morphology and crystallographic orientations. In bimodal two phase (α + β) Ti‐6Al‐2Sn‐4Zr‐2Mo (Ti‐6242) alloys there are often three microstructural lengthscales to consider: large (∼10 μm) equiaxed primary α; >200 nm thick plate α with a basketweave morphology; and very fine scaled (<50 nm plate thickness) secondary α that grows between the larger α plates surrounded by retained β. In this work, we utilise high spatial resolution transmission Kikuchi diffraction (TKD, also known as transmission‐based electron backscatter diffraction, t‐EBSD) and scanning electron microscopy (SEM)‐based forward scattering electron imaging to resolve the structures and orientations of basketweave and secondary α in Ti‐6242. We analyse the α variants formed within one prior β grain, and test whether existing theories of habit planes of the phase transformation are upheld. Our analysis is important in understanding both the thermomechanical processing strategy of new bimodal two‐phase titanium alloys, as well as the ultimate performance of these alloys in complex loading regimes such as dwell fatigue. Our paper champions the significant increase in spatial resolution afforded using transmission techniques, combined with the ease of SEM‐based analysis using conventional electron backscatter diffraction (EBSD) systems and forescatter detector (FSD) imaging, to study the nanostructure of real‐world engineering alloys.

Direct mapping and characterization of dry etch damage-induced PN junction for long-wavelength HgCdTe infrared detector arrays.

Mercury cadmium telluride is the standard material to fabricate high-performance infrared focal plane array (FPA) detectors. However, etch-induced damage is a serious obstacle for realizing highly uniform and damage-free FPA detectors. In this Letter, the high signal-to-noise ratio and high spatial resolution scanning photocurrent microscopy (SPCM) is used to characterize the dry etch-induced inversion layer of vacancy-doped p-type Hg1-xCdxTe (x=0.22) material under different etching temperatures. It is found that the peak-to-peak magnitude of the SPCM profile decreases with a decrease in etching temperature, showing direct proof of controlling dry etch-induced type conversion. Our work paves the way toward seeking optimal etching processes in large-scale infrared FPAs.

In vivo gastrointestinal drug-release monitoring through second near-infrared window fluorescent bioimaging with orally delivered microcarriers

Non-invasive monitoring of gastrointestinal drug release in vivo is extremely challenging because of the limited spatial resolution and long scanning time of existing bioimaging modalities, such as X-ray radiation and magnetic resonance. Here, we report a novel microcarrier that can retain drugs and withstand the harsh conditions of gastrointestinal tract. Significantly, we can track the microcarrier fate and semi-quantitatively monitor the content of drug released in vivo in real time by measuring the fluorescence signals in the second near-infrared window of lanthanide-based downconversion nanoparticles with an absorption competition-induced emission bioimaging system. The microcarriers show a prolonged residence time of up to 72 h in the gastrointestinal tract, releasing up to 62% of their content. Moreover, minimal deposition of the microcarriers is found in non-target organs, such as the liver, spleen and kidney. These findings provide novel insights for the development of therapeutic and bioimaging strategies of orally administered drugs.

Nano-scale chemical evolution in a proton-and neutron-irradiated Zr alloy

Proton-and neutron-irradiated Zircaloy-2 are compared in terms of the nano-scale chemical evolution within second phase particles (SPPs) Zr(Fe,Cr)2 and Zr2(Fe,Ni). This is accomplished through ultra-high spatial resolution scanning transmission electron microscopy and the use of energy-dispersive X-ray spectroscopic methods. Fe-depletion is observed from both SPP types after irradiation with both irradiative species, but is heterogeneous in the case of Zr(Fe,Cr)2, predominantly from the edge region, and homogeneously in the case of Zr2(Fe,Ni). Further, there is evidence of a delay in the dissolution of the Zr2(Fe,Ni) SPP with respect to the Zr(Fe,Cr)2. As such, SPP dissolution results in matrix supersaturation with solute under both irradiative species and proton irradiation is considered well suited to emulate the effects of neutron irradiation in this context. The mechanisms of solute redistribution processes from SPPs and the consequences for irradiation-induced growth phenomena are discussed.

Probing deep photospheric layers of the quiet Sun with high magnetic sensitivity

Context. Investigations of the magnetism of the quiet Sun are hindered by extremely weak polarization signals in Fraunhofer spectral lines. Photon noise, straylight, and the systematically different sensitivity of the Zeeman effect to longitudinal and transversal magnetic fields result in controversial results in terms of the strength and angular distribution of the magnetic field vector. Aims. The information content of Stokes measurements close to the diffraction limit of the 1.5 m GREGOR telescope is analyzed. We took the effects of spatial straylight and photon noise into account. Methods. Highly sensitive full Stokes measurements of a quiet-Sun region at disk center in the deep photospheric Fe I lines in the 1.56 {\mu}m region were obtained with the infrared spectropolarimeter GRIS at the GREGOR telescope. Noise statistics and Stokes V asymmetries were analyzed and compared to a similar data set of the Hinode spectropolarimeter (SOT/SP). Simple diagnostics based directly on the shape and strength of the profiles were applied to the GRIS data. We made use of the magnetic line ratio technique, which was tested against MHD simulations. Results. About 80% of the GRIS spectra of a very quiet solar region show polarimetric signals above a 3{\sigma} level. Area and amplitude asymmetries agree well with small-scale surface dynamo MHD simulations. The magnetic line ratio analysis reveals ubiquitous magnetic regions in the ten to hundred Gauss range with some concentrations of kilo-Gauss fields. Conclusions. The GRIS spectropolarimetric data at a spatial resolution of 0.40" are so far unique in the combination of high spatial resolution scans and high magnetic field sensitivity. Nevertheless, the unavoidable effect of spatial straylight and the resulting dilution of the weak Stokes profiles means that inversion techniques still bear a high risk of misinterpretating the data.

Parallax error in long-axial field-of-view PET scanners—a simulation study

There is a growing interest in the design and construction of a PET scanner with a very long axial extent. One critical design challenge is the impact of the long axial extent on the scanner spatial resolution properties. In this work, we characterize the effect of parallax error in PET system designs having an axial field-of-view (FOV) of 198 cm (total-body PET scanner) using fully-3D Monte Carlo simulations. Two different scintillation materials were studied: LSO and LaBr3. The crystal size in both cases was 4 × 4 × 20 mm3. Several different depth-of-interaction (DOI) encoding techniques were investigated to characterize the improvement in spatial resolution when using a DOI capable detector. To measure spatial resolution we simulated point sources in a warm background in the center of the imaging FOV, where the effects of axial parallax are largest, and at several positions radially offset from the center. Using a line-of-response based ordered-subset expectation maximization reconstruction algorithm we found that the axial resolution in an LSO scanner degrades from 4.8 mm to 5.7 mm (full width at half max) at the center of the imaging FOV when extending the axial acceptance angle (α) from ±12° (corresponding to an axial FOV of 18 cm) to the maximum of ±67°—a similar result was obtained with LaBr3, in which the axial resolution degraded from 5.3 mm to 6.1 mm. For comparison we also measured the degradation due to radial parallax error in the transverse imaging FOV; the transverse resolution, averaging radial and tangential directions, of an LSO scanner was degraded from 4.9 mm to 7.7 mm, for a measurement at the center of the scanner compared to a measurement with a radial offset of 23 cm. Simulations of a DOI detector design improved the spatial resolution in all dimensions. The axial resolution in the LSO-based scanner, with α = ± 67°, was improved from 5.7 mm to 5.0 mm by incorporating a two-layer DOI detector. These results characterize the maximum axial blurring for a fully open 2 m long PET scanner and demonstrate that large sensitivity gains are possible with a modest reduction in resolution when using current clinical detector technology with no DOI capability.