Spatial Organization Research Articles

Assessment of Healthcare Buildings in Terms of Spatial Requirements in Disaster Cases Together with Healthcare Professionals: Adana City Hospital Emergency Department During the Covid-19 Pandemic

From the past to the present, various pandemics have affected societies to varying degrees, leading to changes in various fields such as social, cultural, and architectural aspects. In this context, the study examines the specific arrangements for the COVID-19 pandemic in the emergency department of a city hospital, conducted in January 2022. The aim is to develop a methodology for questioning the emergency department based on evidence and experience regarding flexibility, spatial adequacy, space organization, and isolation in extraordinary situations such as pandemics and to provide design input for the future. For this purpose, a questionnaire form and a space evaluation form were created.In order to benefit from field experiences, a survey form was administered to 137 healthcare professionals working in the emergency department who are directly affected by spatial design. The authors, who are architects, evaluated the space of the emergency department by filling out the "space evaluation form." The findings were evaluated comparatively.The study found that the emergency department's spatial performance was adequate in terms of evaluations related to flexibility, spatial adequacy, and spatial organization but inadequate in terms of evaluations related to isolation. The design elements causing this situation were discussed. The aim is that the findings obtained will be data for hospital managers and designers in the design of future emergency services and the organization of existing emergency services.

Insight into chromatin compaction and spatial organization in rice interphase nuclei

Chromatin organization and its interactions are essential for biological processes, such as DNA repair, transcription, and DNA replication. Detailed cytogenetics data on chromatin conformation, and the arrangement and mutual positioning of chromosome territories in interphase nuclei are still widely missing in plants. In this study, level of chromatin condensation in interphase nuclei of rice (Oryza sativa) and the distribution of chromosome territories (CTs) were analyzed. Super-resolution, stimulated emission depletion (STED) microscopy showed different levels of chromatin condensation in leaf and root interphase nuclei. 3D immuno-FISH experiments with painting probes specific to chromosomes 9 and 2 were conducted to investigate their spatial distribution in root and leaf nuclei. Six different configurations of chromosome territories, including their complete association, weak association, and complete separation, were observed in root meristematic nuclei, and four configurations were observed in leaf nuclei. The volume of CTs and frequency of their association varied between the tissue types. The frequency of association of CTs specific to chromosome 9, containing NOR region, is also affected by the activity of the 45S rDNA locus. Our data suggested that the arrangement of chromosomes in the nucleus is connected with the position and the size of the nucleolus.

The interplay of Pseudomonas aeruginosa and Staphylococcus aureus in dual-species biofilms impacts development, antibiotic resistance and virulence of biofilms in in vitro wound infection models.

Due to high tolerance to antibiotics and pronounced virulence, bacterial biofilms are considered a key factor and major clinical challenge in persistent wound infections. They are typically composed of multiple species, whose interactions determine the biofilm's structural development, functional properties and thus the progression of wound infections. However, most attempts to study bacterial biofilms in vitro solely rely on mono-species populations, since cultivating multi-species biofilms, especially for prolonged periods of time, poses significant challenges. To address this, the present study examined the influence of bacterial composition on structural biofilm development, morphology and spatial organization, as well as antibiotic tolerance and virulence on human skin cells in the context of persistent wound infections. By creating a wound-mimetic microenvironment, the successful cultivation of dual-species biofilms of two of the most prevalent wound pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, was realized over a period of 72 h. Combining quantitative analysis with electron microscopy and label-free imaging enabled a comprehensive evaluation of the dynamics of biofilm formation and matrix secretion, revealing a twofold increased maturation of dual-species biofilms. Antibiotic tolerance was comparable for both mono-species cultures, however, dual-species communities showed a 50% increase in tolerance, mediated by a significantly reduced penetration of the applied antibiotic into the biofilm matrix. Further synergistic effects were observed, where dual-species biofilms exacerbated wound healing beyond the effects observed from either Pseudomonas or Staphylococcus. Consequently, predicting biofilm development, antimicrobial tolerance and virulence for multi-species biofilms based solely on the results from mono-species biofilms is unreliable. This study underscores the substantial impact of a multi-species composition on biofilm functional properties and emphasizes the need to tailor future studies reflecting the bacterial composition of the respective in vivo situation, leading to a more comprehensive understanding of microbial communities in the context of basic microbiology and the development of effective treatments.

Social and Pedagogical Structure of Personality and the Mature Women’s Posture Characteristics.

Topicality. The issue of preserving the mature women health is a global one. Sientific knowledge, represented in a research studies on problems related to health, reflects the understanding of the latter in the plane of the human body spatial organization, taking into account the reality of the new millennium. Scholars note that the highest rate of morbidity amid diseases of the circulatory system, the second place is occupied by diseases of the musculoskeletal system. The reserch purpose is to determine the socio-pedagogical structure of the personality and the most frequent violations of the womens` posture aged 36-40. The following research methods as theoretical analysis, photography and posture analysis, pedagogical and mathematical methods have been used over the study. The research results. The vast majority of women, namely 81.81%, taking part in the ascertainment experiment, have a diploma of higher education, 31.81% of women have officially married, 31.81% are in a civil marriage, 27.29% have divorced, 9.09% have never married. By social status, women were distributed as follows: 27.26% are employees of commercial structures, 22.74% are private entrepreneurs, 27.26% are office workers and 22.74% – housewives. Considering the social status of women with their differentiation by age groups, such following data have been obtained: among women aged 36–37, 20.0% are employees of commercial structures, 20.0% are entrepreneurs, 30.0% – office workers, 30.0% – housewives; among women aged 38–40, 33.33% are employees of commercial structures, 25.0% are entrepreneurs, 25.0% are office workers, 16.67% – housewives. Findings. It was found that among women aged 36-37 years, 23.08% demonstrate a healthy type of posture, 38.46% of women have got a scoliotic posture, and 38.46% – a kyphosis type; among women 38-40 years old, 16.67% have a healthy type of posture, 38.88% – a scoliotic posture, and 44.44% – a kyphosis type of posture.

Transcriptional Hubs Within Cliques in Ensemble Hi-C Chromatin Interaction Networks.

Chromatin conformation capture technologies permit the study of chromatin spatial organization on a genome-wide scale at a variety of resolutions. Despite the increasing precision and resolution of high-throughput chromatin conformation capture (Hi-C) methods, it remains challenging to conclusively link transcriptional activity to spatial organizational phenomena. We have developed a clique-based approach for analyzing Hi-C data that helps identify chromosomal hotspots that feature considerable enrichment of chromatin annotations for transcriptional start sites and, building on previously published work, show that these chromosomal hotspots are not only significantly enriched in RNA polymerase II binding sites as identified by the ENCODE project, but also identify a noticeable increase in FANTOM5 and GTEx transcription within our identified cliques across a variety of tissue types. From the obtained data, we surmise that our cliques are a suitable method for identifying transcription factories in Hi-C data, and outline further extensions to the method that may make it useful for locating regions of increased transcriptional activity in datasets where in-depth expression or polymerase data may not be available.

BIM: A COLLABORATIVE APPROACH TO ARCHITECTURAL PROJECT MANAGEMENT

At the present time, digital technology has fundamentally altered both our daily lives and professional environments. New tools and software have emerged, becoming crucial across various fields, including urban development, by replacing traditional methods. Among these technological advancements is Building Information Modeling (BIM), also known as Better Information Management, which has evolved into a collaborative management process integrating architectural projects. BIM relies on technologies and solutions that intelligently model and structure the different stakeholders involved in a project. It creates a virtual environment that accurately represents buildings and urban spaces, including their geometry, spatial organizations, functionalities, and other significant information. This contribution aims to examine the challenges associated with using BIM as a collaborative project management process in architecture. It also seeks to explore new strategies that promote the efficiency and quality of architectural projects.

СУЧАСНІ ПІДХОДИ ДО ПРОСТОРОВОЇ ОРГАНІЗАЦІЇ МУЗЕЇВ ТА ЕКСПОЗИЦІЙ ПРОСТО НЕБА. ЄВРОПЕЙСЬКИЙ ДОСВІД

There are numerous open-air museums worldwide, and public spaces often incorporate both permanent and temporary outdoor exhibitions. Based on studies conducted on open-air museums and exhibitions in Vienna and its surrounding areas, as part of the academic cooperation program TU Vienna – TU Lviv, this article explores the European approach to the architectural and spatial organization of such institutions. Through this research, contemporary approaches to their architectural and spatial organization were examined, and both positive and negative aspects were highlighted. This article aims to analyze the distinct features of the architectural and spatial organization of European open-air museums and exhibitions, identifying their positive aspects and approaches that could be implemented in Ukraine. In Ukrainian studies, the topic of modern open-air museums is only gaining popularity and is not well presented. Hence, involving local scholars in this globally expanding field would be relevant. European open-air museums, particularly in Austria, show several trends in spatial organization, including a comprehensive approach, accessibility, family-oriented infrastructure, wide implementation of interactive elements, and integration of multimedia technologies. These museums blend original artifacts with modern enhancements to provide visitors with a unique experience. Noteworthy aspects observed in open-air museums and exhibitions, which are scarce or absent in Ukraine, include combining several neighboring objects into one museum complex, a large number of children's playgrounds, ample recreation and food areas, inclusiveness, infrastructure for dog walking, provision of venues for holding mass events, and onsite production and sale of themed merchandise in souvenir shops. The principles of spatial and architectural organization in open-air museums and exhibitions in Vienna and its vicinity boast several positive features that we believe are pertinent for implementation in Ukraine. Notably, interactivity to engage visitors and inclusivity, which is poised to be a pressing issue during Ukraine's post-war reconstruction, stand out as crucial aspects. The findings from this study can serve as a foundation for enhancing existing or designing new open-air museums in Ukraine.

Exploring the neural basis of non-invasive prehabilitation in brain tumour patients: An fMRI-based case report of language network plasticity

Primary brain neoplasms are associated with elevated mortality and morbidity rates. Brain tumour surgery aims to achieve maximal tumour resection while minimizing damage to healthy brain tissue. Research on Neuromodulation Induced Cortical Prehabilitation (NICP) has highlighted the potential, before neurosurgery, of establishing new brain connections and transfer functional activity from one area of the brain to another. Nonetheless, the neural mechanisms underlying these processes, particularly in the context of space-occupying lesions, remain unclear. A patient with a left frontotemporoinsular tumour underwent a prehabilitation protocol providing 20 sessions of inhibitory non-invasive neuromodulation (rTMS and multichannel tDCS) over a language network coupled with intensive task training. Prehabilitation resulted in an increment of the distance between the tumour and the language network. Furthermore, enhanced functional connectivity within the language circuit was observed. The present innovative case-study exposed that inhibition of the functional network area surrounding the space-occupying lesion promotes a plastic change in the network’s spatial organization, presumably through the establishment of novel functional pathways away from the lesion’s site. While these outcomes are promising, prudence dictates the need for larger studies to confirm and generalize these findings.

Hypoxia coordinates the spatial landscape of myeloid cells within glioblastoma to affect survival.

Myeloid cells are highly prevalent in glioblastoma (GBM), existing in a spectrum of phenotypic and activation states. We now have limited knowledge of the tumor microenvironment (TME) determinants that influence the localization and the functions of the diverse myeloid cell populations in GBM. Here, we have utilized orthogonal imaging mass cytometry with single-cell and spatial transcriptomic approaches to identify and map the various myeloid populations in the human GBM tumor microenvironment (TME). Our results show that different myeloid populations have distinct and reproducible compartmentalization patterns in the GBM TME that is driven by tissue hypoxia, regional chemokine signaling, and varied homotypic and heterotypic cellular interactions. We subsequently identified specific tumor subregions in GBM, based on composition of identified myeloid cell populations, that were linked to patient survival. Our results provide insight into the spatial organization of myeloid cell subpopulations in GBM, and how this is predictive of clinical outcome.

O13 Benchmarking a human pluripotent stem cell-derived hair-bearing skin organoid model to prenatal skin

Abstract Introduction and aims Molecular regulation of mammalian skin development has been largely derived from murine studies. The lack of human studies stems primarily from the difficulties in prenatal human skin access. There is an important need to study human prenatal skin owing to the notable differences between human and mouse skin and the paucity of mechanistic understanding of congenital skin disorders. Methods We assembled the first comprehensive multiomic reference atlas of prenatal human skin (21 samples from 7 to 16 postconception weeks), combining single-cell (235 201 cells) and spatial transcriptomic data. We followed 84 skin organoids (SkOs) using single-cell analysis over a 5-month culture period and characterized the spatial organization of the skin in mature SkOs. Prenatal skin, SkO and any relevant pre-existing single-cell RNA-Seq datasets were integrated into the analysis. Cell–cell interactions and cell-lineage determination were explored using CellPhoneDB and trajectory inferences. We evaluated the differentiation trajectory alignment between prenatal skin and SkO using the Genes2Genes analysis. Results Our findings reveal predicted factors regulating the epithelial–mesenchymal crosstalk during human hair follicle formation; progressive expression of scar-promoting genes in later gestation, potentially contributing to scarless healing properties of human prenatal skin observed during the early stages of gestation and expression of genes causing genetic skin disorders in the same cell types in prenatal human skin and SkO. We also found that SkOs morphologically recapitulate human skin development from early (periderm formation and shedding) to late (cornification and hair follicle formation) stages. We also demonstrated that SkO and prenatal skin mesenchyme differentiation into hair follicle dermal condensate and dermal papilla is highly conserved at the cellular and molecular level. Conclusions SkOs faithfully recapitulate human prenatal skin development and are a valuable model for mechanistic studies on skin development including hair follicle biology and congenital skin disorders.

Analyzing the spatial organization of the Chechen Republic's economy

Subject. This article discusses the issues of industrial development of the Chechen Republic and efficient use of resources. Objectives. The article aims to comprehensively assess the economic potential of the plains and mountainous areas of the Chechen Republic, and search for ways to optimize the spatial organization of economic activities in the region. Methods. For the study, I used economic and statistical research methods. Results. The article finds certain imbalances of the spatial distribution of economic activities in the Chechen Republic and states that the mining and processing industries are thinly represented in the Chechen Republic's economy. Conclusions. To accelerate the economic advancement of the Chechen Republic, it is necessary to create a modern science-intensive industrial base. The development of industry is closely related to agglomeration processes. To solve the identified problems in the spatial organization of economic activities of the Chechen Republic, it is necessary to disperse production among municipalities in accordance with the potential capabilities of the areas, excluding significant imbalances.

On the evolution of chromosomal regions with high gene strand bias in bacteria.

On circular bacterial chromosomes, the majority of genes are coded on the leading strand. This gene strand bias (GSB) ranges from up to 85% in some Bacillota to a little more than 50% in other phyla. The factors determining the extent of the strand bias remain to be found. Here, we report that species in the phylum Gemmatimonadota share a unique chromosome architecture, distinct from neighboring phyla: in a conserved 600-kb region around the terminus of replication, almost all genes were located on the leading strands, while on the remaining part of the chromosome, the strand preference was more balanced. The high strand bias (HSB) region harbors the rRNA clusters, core, and highly expressed genes. Selective pressure for reduction of collisions with DNA replication to minimize detrimental mutations can explain the conservation of essential genes in this region. Repetitive and mobile elements are underrepresented, suggesting reduced recombination frequency by structural isolation from other parts of the chromosome. We propose that the HSB region forms a distinct chromosomal domain. Gemmatimonadota chromosomes evolved mainly by expansion through horizontal gene transfer and duplications outside of the ancient high strand bias region. In support of our hypothesis, we could further identify two Spiroplasma strains on a similar evolutionary path.IMPORTANCEOn bacterial chromosomes, a preferred location of genes on the leading strand has evolved to reduce conflicts between replication and transcription. Despite a vast body of research, the question why bacteria show large differences in their gene strand bias is still not solved. The discovery of "hybrid" chromosomes in different phyla, including Gemmatimonadota, in which a conserved high strand bias is found exclusively in a region at ter, points toward a role of nucleoid structure, additional to replication, in the evolution of strand preferences. A fine-grained structural analysis of the ever-increasing number of available bacterial genomes could help to better understand the forces that shape the sequential and spatial organization of the cell's information content.

The role of compartmentalized β-AR/cAMP signaling in the regulation of lipolysis in white and brown adipocytes.

White and brown adipocytes are central mediators of lipid metabolism and thermogenesis, respectively. Their function is tightly regulated by all three β-adrenergic receptor (β-AR) subtypes which are coupled to the production of the second messenger 3',5'-cyclic adenosine monophosphate (cAMP). While known for decades in other cell types, compartmentation of adipocyte β-AR/cAMP signaling by spatial organization of the pathway and by cAMP degrading phosphodiesterases (PDEs) as well as its role in the regulation of lipolysis is only beginning to emerge. Here, we provide a short overview of recent findings which shed light on compartmentalized signaling using live cell imaging of cAMP in adipocytes and discuss possible future directions of research which could open up new avenues for the treatment of metabolic disorders.

The sustainable potential of the architectural and urban heritage of the Algerian ksour

ABSTRACT This paper aims to assess the sustainability potential of Algerian desert Ksour's architectural and urban heritage. It identifies systems aligning with the 17 sustainable development goals (SDGs), categorized into formal, spatial organization, and architectonic aspects. The approach correlates Ksour's architectural features with SDGs defined by the International Union of Architects, yielding 40 passive features applicable for achieving SDGs. Notably, features like high windows (architectural) and strategic siting (urban) remain relevant for energy efficiency and carbon footprint reduction. This research gives us the opportunity to improve our strategies for rehabilitating and adapting built heritage so that it can meet current environmental challenges, but also opens up the possibility in the future of continuing to identify more sustainable systems by extending the study of heritage examples, but also and above all of developing a reflection on the adaptation of these passive systems in the design of contemporary architectural and urban projects.

Quantifying 3′UTR length from scRNA-seq data reveals changes independent of gene expression

Although more than half of all genes generate transcripts that differ in 3′UTR length, current analysis pipelines only quantify the amount but not the length of mRNA transcripts. 3′UTR length is determined by 3′ end cleavage sites (CS). We map CS in more than 200 primary human and mouse cell types and increase CS annotations relative to the GENCODE database by 40%. Approximately half of all CS are used in few cell types, revealing that most genes only have one or two major 3′ ends. We incorporate the CS annotations into a computational pipeline, called scUTRquant, for rapid, accurate, and simultaneous quantification of gene and 3′UTR isoform expression from single-cell RNA sequencing (scRNA-seq) data. When applying scUTRquant to data from 474 cell types and 2134 perturbations, we discover extensive 3′UTR length changes across cell types that are as widespread and coordinately regulated as gene expression changes but affect mostly different genes. Our data indicate that mRNA abundance and mRNA length are two largely independent axes of gene regulation that together determine the amount and spatial organization of protein synthesis.

A Spatial Transcriptomics Browser for Discovering Gene Expression Landscapes across Microscopic Tissue Sections.

A crucial feature of life is its spatial organization and compartmentalization on the molecular, cellular, and tissue levels. Spatial transcriptomics (ST) technology has opened a new chapter of the sequencing revolution, emerging rapidly with transformative effects across biology. This technique produces extensive and complex sequencing data, raising the need for computational methods for their comprehensive analysis and interpretation. We developed the ST browser web tool for the interactive discovery of ST images, focusing on different functional aspects such as single gene expression, the expression of functional gene sets, as well as the inspection of the spatial patterns of cell-cell interactions. As a unique feature, our tool applies self-organizing map (SOM) machine learning to the ST data. Our SOM data portrayal method generates individual gene expression landscapes for each spot in the ST image, enabling its downstream analysis with high resolution. The performance of the spatial browser is demonstrated by disentangling the intra-tumoral heterogeneity of melanoma and the microarchitecture of the mouse brain. The integration of machine-learning-based SOM portrayal into an interactive ST analysis environment opens novel perspectives for the comprehensive knowledge mining of the organization and interactions of cellular ecosystems.

SEM Image Processing Assisted by Deep Learning to Quantify Mesoporous γ-Alumina Spatial Heterogeneity and Its Predicted Impact on Mass Transfer.

The pore network architecture of porous heterogeneous catalyst supports has a significant effect on the kinetics of mass transfer occurring within them. Therefore, characterizing and understanding structure-transport relationships is essential to guide new designs of heterogeneous catalysts with higher activity and selectivity and superior resistance to deactivation. This study combines classical characterization via N2 adsorption and desorption and mercury porosimetry with advanced scanning electron microscopy (SEM) imaging and processing approaches to quantify the spatial heterogeneity of γ-alumina (γ-Al2O3), a catalyst support of great industrial relevance. Based on this, a model is proposed for the spatial organization of γ-Al2O3, containing alumina inclusions of different porosities with respect to the alumina matrix. Using original, advanced SEM image analysis techniques, including deep learning semantic segmentation and porosity measurement under gray-level calibration, the inclusion volume fraction and interphase porosity difference were identified and quantified as the key parameters that served as input for effective tortuosity factor predictions using effective medium theory (EMT)-based models. For the studied aluminas, spatial porosity heterogeneity impact on the effective tortuosity factor was found to be negligible, yet it was proven to become significant for an inclusion content of at least 30% and an interphase porosity difference of over 20%. The proposed methodology based on machine-learning-supported image analysis, in conjunction with other analytical techniques, is a general platform that should have a broader impact on porous materials characterization.

Tropical cyclone low-level wind speed, shear, and veer: sensitivity to the boundary layer parametrization in the Weather Research and Forecasting model

Abstract. Mesoscale modeling can be used to analyze key parameters for wind turbine load assessment in a large variety of tropical cyclones. However, the modeled wind structure of tropical cyclones is known to be sensitive to the boundary layer scheme. We analyze modeled wind speed, shear, and wind veer across a wind turbine rotor plane in the eyewall and outer cyclone. We further assess the sensitivity of wind speed, shear, and veer to the boundary layer parametrization. Three model realizations of Typhoon Megi are analyzed over the open ocean using three frequently used boundary layer schemes in the Weather Research and Forecasting (WRF) model. All three typhoon simulations reasonably reproduce the cyclone track and structure. The boundary layer parametrization causes up to 15 % differences in median wind speed at hub height between the simulations. The simulated wind speed variability also depends on the boundary layer scheme. The modeled median wind shear is smaller than or equal to 0.11 used in the current IEC (International Electrotechnical Commission) standard regardless of the boundary layer scheme for the eyewall and outer cyclone region. However, up to 43.6 % of the simulated wind profiles in the eyewall region exceed 0.11. While the surface inflow angle is sensitive to the boundary layer scheme, wind veer in the lowest 400 m of the atmospheric boundary layer is less affected by the boundary layer scheme. Simulated median wind veer reaches values up to 1.7×10-2° m−1 (1.2×10-2° m−1) in the eyewall region (outer cyclone region) and is relatively small compared to moderate-wind-speed regimes. On average, simulated wind speed shear and wind veer are highest in the eyewall region. Yet strong spatial organization of wind shear and veer along the rainbands may increase wind turbine loads due to rapid changes in the wind profile at the turbine location.

Practice-induced SNARC: evidence from a null-SNARC sample.

The mental representation of numbers inherently involves a spatial organization, often positioning smaller numbers to the left and larger numbers to the right. The SNARC effect, characterized by faster responses to small numbers using the left hand and vice versa for large numbers, is typically attributed to this left-to-right oriented mental number line (MNL). However, the direction of the SNARC effect seems to rely on reading direction, with most research exploring these mechanisms conducted within left-to-right reading cultures where the SNARC effect is prevalent. This study takes advantage of a sample from a left-to-right reading culture that does not exhibit the SNARC effect, allowing us to isolate and elucidate the stand-alone effects of recent experiences on SNARC. Therefore, the current study aims to investigate how MNL-compatible and MNL-incompatible practices induce an effect within a sample lacking the SNARC effect. To accomplish this, we reinvited the individuals from the sample which had previously shown no SNARC, and retested those who agreed to take part in the current study after an MNL-compatible or MNL-incompatible practice manipulation. The findings revealed an absence of the SNARC effect with MNL-compatible practices. Conversely, MNL-incompatible practices yielded a reverse SNARC effect. These results prompt a discussion on SNARC mechanisms within the framework of practice effects.

Spatial mapping of hepatic ER and mitochondria architecture reveals zonated remodeling in fasting and obesity

The hepatocytes within the liver present an immense capacity to adapt to changes in nutrient availability. Here, by using high resolution volume electron microscopy, we map how hepatic subcellular spatial organization is regulated during nutritional fluctuations and as a function of liver zonation. We identify that fasting leads to remodeling of endoplasmic reticulum (ER) architecture in hepatocytes, characterized by the induction of single rough ER sheet around the mitochondria, which becomes larger and flatter. These alterations are enriched in periportal and mid-lobular hepatocytes but not in pericentral hepatocytes. Gain- and loss-of-function in vivo models demonstrate that the Ribosome receptor binding protein1 (RRBP1) is required to enable fasting-induced ER sheet-mitochondria interactions and to regulate hepatic fatty acid oxidation. Endogenous RRBP1 is enriched around periportal and mid-lobular regions of the liver. In obesity, ER-mitochondria interactions are distinct and fasting fails to induce rough ER sheet-mitochondrion interactions. These findings illustrate the importance of a regulated molecular architecture for hepatocyte metabolic flexibility.