This study investigated the relationship between spatial and musical intelligences and learning styles of EFL learners and their vocabulary knowledge. Accordingly, relationship between spatial intelligence, musical intelligence and vocabulary knowledge, visual learning style, auditory learning style, and kinesthetic learning style with vocabulary knowledge, listening and vocabulary knowledge, and finally spatial, musical intelligence, visual, auditory kinesthetic learning style as independent variables and vocabulary knowledge s dependent variable was examined. This study is an experimental and applied research using four texts to specify participants intelligence their learning styles, vocabulary knowledge and listening knowledge. For this reason, four texts including MIDAS Test of Shearer (1996) the Persian of Spatial, and Musical Intelligence VAK Learning Style Test (Visual, Auditory, Kinesthetic), Vocabulary Levels Test of Nation (2001) One listening Test from the IELTS 5 book were applied. In this study, 200 Iranian senior BA EFL learners from Islamic Azad Universities of Tehran, Male and Female, 22 to 30 years old, majoring in TEFL were examined. Result of data analysis showed that there is a significant relationship between spatial and musical intelligences and learning styles of Iranian EFL learners and their vocabulary knowledge. Also, multiple intelligence plays a significant role in learning vocabulary, as the nature of intelligence represents this issue and shows that learning is a psychological issue and human’s different aspects of learning depends of different aspect of intelligence.