The paper presents an analysis the spatial distribution of turbulent kinetic energy (TKE) for bolt fishways, including the impact of additional spillway slots and fixed channel development. The research was done for two models, each containing a different arrangement of slots. The presented results of research for bolt fishways were obtained as an effect of laboratory tests. The measurements were done for three components of instant flow velocity magnitude (speed). Analysis of the results was done for a 3D flow structure using Matlab software. In the case of bolt fishways, significant differences were noted for the method of velocity and TKE distribution, in reference to research comprising channels with biological development. It was stated that a reason for this is the flexible development of the channel. The occurrence of extreme TKE values in the chamber (pool) is strictly associated with the characteristics of interaction zones between various flow structures. It was also stated that the lower the parapet of the slot’s spillway shelf is in the fishway’s partition, the higher TKE could be expected just downstream of the section. These establishments may be important for the designing process in the case of fish passes of various types of construction.