Spatial Distribution Of Bacterial Communities Research Articles

Spatial distribution of bacterial communities driven by environmental factors in the sediment of Lateolabrax japonicus ponds

Dynamic changes of bacterial communities in the sediment are very important for preventing diseases and water quality in aquaculture ponds. In this study, bacterial communities in the sediment of Sea perch Lateolabrax japonicus, an important farmed fish in China, ponds were studied using 16S rRNA Illumina sequencing and functional gene array. At the phylum level, Pseudomonadota, Actinomycetota and Chloroflexota were the dominant species. The bacterial community composition was different in spatial variation. Among the environmental factors, the concentration of nitrogen related factors (TN, NO3−-N NH3-N, NO2−N) and total organic carbon was the main impact on the composition of bacterial community, especially denitrifying bacterial community. Nitrifying and denitrifying bacteria accounted for 4.81 %, and bacteria related to nitrogen cycle accounted for 18.36 %. Higher diversity of bacteria in healthy fish pond than that of the diseased fish pond. In the diseased fish pond, lower abundance of Nitrosospira and higher abundance of Nitrobacter were found, and the contents of NH3-N, NO2−-N and total organic carbon were significantly higher. This study would help to reveal that microbial community structure could reflect the health of cultured animal and surroundings, and it could provide a theoretical reference for the healthy environments in the sediment of Sea perch ponds in South China.

Bacterial Diversity and Vertical Distribution Patterns in Sandy Sediments: A Study on the Bacterial Community Structure Based on Environmental Factors in Tributaries of the Yangtze River.

Bacterial diversity and its distribution characteristics in sediments are critical to understanding and revealing biogeochemical cycles in sediments. However, little is known about the relationship between biogeochemistry processes and vertical spatial distribution of bacterial communities in sandy sediments. In this study, we used fluorescence quantitative PCR, high-throughput sequencing technology and statistical analysis to explore the vertical distribution pattern of bacterial community diversity and its influencing factors in sandy sediments of the Yangtze River Basin. The aim is to enrich the understanding of the ecological characteristics and functions of bacteria in river ecosystems. The results showed that both sediment bacterial abundance and diversity showed a gradual decrease from surface to bottom in the vertical distribution. The main environmental factors that influenced the bacterial distribution pattern were pore water dissolved oxygen (DO), total nitrogen (TN) concentration and sediment nitrogen (N) content. The dominant bacterial species, Massilia and Flavobacterium, are suitable for growth and reproduction in high oxygen and nutrient-richer environments, while Limnobacter prefers low oxygen or anaerobic conditions. The vertical distribution pattern of bacteria and its influencing factors in river sandy sediment found in this study differ from the results in mud sediment, which may be related to the larger granular gap between sandy sediment and the lower content of organic matter. The findings of this study further our understanding of the distribution patterns and ecological preferences of microbial communities in river sediments, providing insights into how these communities may adapt to varying environmental conditions.

Spatial distribution of bacterial communities driven by multiple environmental factors in sediment of brackish channel catfish ponds in Eastern China

Sediment bacteria are crucial for maintaining water quality, preventing disease, and cycling nutrients in aquaculture ponds. In the present study, a total of 42 sediment samples were collected over the course of four months from three farms. By using 16S rRNA high-throughput sequencing techniques, bacterial communities were studied. Proteobacteria, Chloroflexi, Desulfobacterota, Firmicutes, Actinobacteriota, Bacteroidetes and Acidobacteriota were the dominant species at the phylum level in all samples. Spatial variation had a greater impact on the bacterial community composition and distribution of brackish pond sediments. Chloroflexi, Desulfobacterota, Spirochaetota and Latescibacterota were all substantially correlated positively with salinity. Moreover, salinity was negatively correlated with beta diversity. Salinity and total organic carbon were positively correlated with bacterial community distribution. This study was helpful to understand the response of bacterial communities to habitat changes, and provided a theoretical reference for the environmental supervision of aquaculture in sediment of brackish channel catfish ponds in Eastern China.

Impacts of Thermal Drainage on Bacterial Diversity and Community Construction in Tianwan Nuclear Power Plant.

As one of the low-carbon and high-efficient energy sources, nuclear power is developing vigorously to alleviate the crisis of global climate warming and realize carbon neutrality goals. Meanwhile, the ecological effect of thermal drainage in the nuclear power plant is significantly remarkable, which environmental assessment system has not yet referred to microorganisms. The rapid response of microbial diversity and community structure to environmental changes is crucial for ecosystem stability. This study investigated the bacterial diversity, community construction, and the co-occurrence patterns by 16S rRNA gene amplicon sequencing among gradient warming regions in Tianwan Nuclear Power Plant. The alpha diversity of the high warming region was the lowest in summer, which was dominated by Proteobacteria, whereas the highest bacterial diversity presented in high warming regions in winter, which harbored higher proportions of Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. The spatial distribution of bacterial communities showed clear separation especially in summer. Strong correlations were between community compositions and environmental factors, such as salinity, DO, TN, and temperature in summer. Furthermore, remarkable seasonality in bacterial co-occurrence patterns was discovered: the robustness of the bacterial co-occurrence network was promoted in winter, while the complexity and robustness were decreased in summer due to the warming of thermal drainage. These findings reveal the potential factors underpinning the influence of thermal drainage on bacterial community structure, which make it possible to predict the ecological effect of the nuclear power plants by exploring how the microbial assembly is likely to respond to the temperature and other environmental changes.

Distribution patterns of benthic bacteria and nematode communities in estuarine sediments

Benthic organisms are crucial in the regulation of the ecosystem functions. The interactions between benthic nematodes and sediment bacteria across divergent environmental conditions are poorly understood. The main goal of this study was to understand the spatial distribution patterns and diversity of benthic bacterial communities and nematode assemblages of the intertidal sediments in three sampling sites (Navigator, Tróia and Moinho) along Sado Estuary (SW, Portugal). Bacterial communities were described using a 16S metagenomic approach, while nematode assemblages were characterized using morphological identification. Overall, bacterial and nematode communities presented significant diversity between sites (p < 0.05), which is primarily related with the environmental variables (e.g., organic matter and percentage of gravel). The spatial distribution of bacterial communities was in accordance with the ecological conditions of three selected sites at a larger scale than nematode assemblages. Previously described as good ecological indicators, nematode assemblages were separated at sampling site level, suggesting that their response is driven by within site specific factors at a smaller scale. Hence, the present study set a fundamental ground for future research on functional interactions between bacteria and nematodes.

Habitats modulate influencing factors shaping the spatial distribution of bacterial communities along a Tibetan Plateau riverine wetland

The Tibetan Plateau riverine wetland is very sensitive to global climate change. Understanding the mechanisms that maintain the spatial patterns of bacterial communities provides insight into the dominant biogeochemical processes within the plateau riverine wetlands. Nonetheless, the spatial distribution of bacterial communities along these wetlands has rarely been explored. We investigated the spatial patterns of bacterial community within rhizosphere soil, bulk soil, and sediment samples collected along the Yarlung Tsangpo riverine wetland (YTRW), the longest plateau riverine wetland in China. Our results indicated that the diversity of bacterial communities in all three habitats increased significantly along the YTRW. The slope of the linear relationship between distance and bacterial community diversity in sediment was steeper than those for bulk and rhizosphere soils. Furthermore, bacterial communities in all three habitats showed significant distance–decay relationships. A combination of historical factors (geographical distance and climatic factors) and contemporary environmental heterogeneity (edaphic properties) controlled spatial distributions of bacterial communities in all three habitats, although climatic factors were predominant. Climatic factors affected rhizosphere bacterial communities more than those in bulk soil and sediment. Co-occurrence network analysis revealed that the potential interactions between bacterial taxa may decrease along the YTRW. This field investigation highlighted that the climatic factors strongly influenced the spatial distribution of bacterial communities along the YTRW; however, habitat differences among rhizosphere soil, bulk soil, and sediment samples affected the relative importance of climatic factors on spatial distributions of the associated bacterial communities. These findings would improve the understanding of biogeochemical processes in these typical habitats and potential alterations provoked by climate change.

Distribution of microbial communities in seasonally frozen soil layers on the Tibetan Plateau and the driving environmental factors.

Large stocks of carbon and nitrogen stored in permafrost regions can potentially feed back to global biogeochemical cycles under climate warming. To understand the response of microbial communities to environmental changes, this study investigated the spatial distribution of bacterial communities in the upper layers (0-10, 10-20, and 20-30cm) of seasonally frozen soil on the Tibetan Plateau and their relationships with the environmental factors. A total of 135 soil samples were collected from the soils at depths of 0-10, 10-20, and 20-30cm in the Lhasa River and Nyang River basins, and the diversity and composition of bacterial communities in them were identified by high-throughput 16S rRNA gene sequencing. Bacterial diversity changed significantly with soil depth in the Nyang River basin (p < 0.001), while no obvious change was found in the Lhasa River basin. The whole bacterial composition exhibited small variations across different soil layers (p > 0.05). The relative abundance of aerobic bacteria, Sphingomonas and Arthrobacter, decreased with soil depth, while that of the other aerobic, facultative anaerobic, and anaerobic bacteria did not exhibit this trend. Soil pH was the key driving edaphic factor of the whole bacterial composition in all three depth layers, while vegetation also had an important influence on bacterial composition. Arthrobacter, Bradyrhizobium, and Bacillus had obvious correlations with soil nutrients or vegetation, while the other species were not significantly correlated with any environmental factors. Structural equation modeling revealed that vegetation and mean annual temperature had a key direct impact on the bacterial diversity and composition, respectively. Climate also indirectly affected bacterial communities, mainly through shaping soil pH and vegetation. These results indicate that the soil depth has a different impact on the bacterial α-diversity, whole bacterial composition, and specific taxa in the 0-30-cm surface layers of seasonally frozen soil, which were mainly determined by various environmental factors.

Spatial Distribution of the Pepper Blight (Phytophthora capsici) Suppressive Microbiome in the Rhizosphere.

The properties of plant rhizosphere are dynamic and heterogeneous, serving as different habitat filters for or against certain microorganisms. Herein, we studied the spatial distribution of bacterial communities in the rhizosphere of pepper plants treated with a disease-suppressive or non-suppressive soil. The bacterial richness was significantly (p < 0.05) higher in plants treated with the disease-suppressive soil than in those treated with the non-suppressive soil. Bacterial richness and evenness greatly differed between root parts, with decrease from the upper taproot to the upper fibrous root, the lower taproot, and the lower fibrous root. As expected, the bacterial community in the rhizosphere differed between suppressive and non-suppressive soil. However, the spatial variation (36%) of the bacterial community in the rhizosphere was much greater than that explained by soils (10%). Taxa such as subgroups of Acidobacteria, Nitrosospira, and Nitrospira were known to be selectively enriched in the upper taproot. In vitro Bacillus antagonists against Phytophthora capsici were also preferentially colonized in the taproot, while the genera such as Clostridium, Rhizobium, Azotobacter, Hydrogenophaga, and Magnetospirillum were enriched in the lower taproot or fibrous root. In conclusion, the spatial distribution of bacterial taxa and antagonists in the rhizosphere of pepper sheds light on our understanding of microbial ecology in the rhizosphere.

Rhizosphere impacts bacterial community structure in the tea (Camellia sinensis (L.) O. Kuntze.) estates of Darjeeling, India.

India contributes 28% of the world's tea production, and the Darjeeling tea of India is a world-famous tea variety known for its unique quality, flavour and aroma. This study analyzed the spatial distribution of bacterial communities in the tea rhizosphere of six different tea estates at different altitudes. The organic carbon, total nitrogen and available phosphate were higher in the rhizosphere soils than the bulk soils, irrespective of the sites. Alpha and beta diversities were significantly (p < 0.05) higher in the bulk soil than in the rhizosphere. Among the identified phyla, the predominant ones were Proteobacteria, Actinobacteria and Acidobacteria. At the genus level, only four out of 23 predominant genera (>1% relative abundance) could be classified, viz., Candidatus Solibacter (5.36 ± 0.36%), Rhodoplanes (4.87 ± 0.3%), Candidatus Koribacter (2.3 ± 0.67%), Prevotella (1.49 ± 0.26%). The rhizosphere effect was prominent from the significant depletion of more ASVs (n = 39) compared to enrichment (n = 11). The functional genes also exhibit a similar trend with the enrichment of N2 fixation genes, disease suppression and Acetoin synthesis. Our study reports that the rhizobiome of tea is highly selective by reducing the alpha and beta diversity while enriching the significant functional genes.

Mitigation of Eutrophication in a Shallow Lake: The Influences of Submerged Macrophytes on Phosphorus and Bacterial Community Structure in Sediments

Remediating water eutrophication is critical for maintaining healthy and sustainable development of lakes. The aim of this study was to explore the seasonal variation in phosphorus (P) speciation and bacterial community structure in sediments of Qin Lake (Taizhou, Jiangsu Province, China) associated with the growth of submerged macrophyte Vallisneria natans. The differences in sediment bacterial diversity and community structure between V. natans growing and control areas were analyzed over a period of one year. The results showed that V. natans growth reduced the total P and organic matter contents of the sediments and increased the bioavailable iron (Fe) and Fe-bound P contents. The α-diversity of sediment bacteria was significantly higher in the presence of V. natans than in the controls during the vigorous plant growth stage. In the presence of V. natans, there was a higher relative abundance of Proteobacteria and lower relative abundances of Chloroflexi and Acidobacteria. The Fe(II) content in the sediment had a larger influence on the spatial distribution of bacterial communities than sediment Fe-bound P, organic matter, and Fe(II) contents. V. natans growth could reshape sediment bacterial community structure in the shallow lake, which, in turn, enhanced P immobilization in the sediments and thereby improved the water quality.

Zooming in to acquire micro-reaction: Application of microfluidics on soil microbiome

Microfluidics confers unique advantages in microbiological studies as these devices can accurately replicate the micro- and even nano-scale structures of soil to simulate the habitats of bacteria. It not only helps us understand the spatial distribution of bacterial communities (such as biofilms), but also provides mechanistic insights into microbial behaviors including chemotaxis and horizontal gene transfer (HGT). Microfluidics provides a feasible means for real-time, in situ studies and enables in-depth exploration of the mechanisms of interactions in the soil microbiome. This review aims to introduce the basic principles of microfluidic technology and summarize the recent progress in microfluidic devices to study bacterial spatial distribution and functions, as well as biological processes, such bacterial chemotaxis, biofilm streamers (BS), quorum sensing (QS), and HGT. The challenges in and future development of microfluidics for soil microbiological studies are also discussed.

Distinct microhabitats affect the relative balance of ecological processes shaping the spatial distribution of bacterial communities in lakeshore habitats

Abstract The lakeshore habitat, which is an interface zone between lake and land, harbours various organic particles providing nutrient‐rich microhabitats for bacterial colonisation. Revealing the mechanisms maintaining the bacterial diversity generated on these particles is vital for understanding the biogeochemical processes within lakeshore habitats. To date, however, the bacterial communities within lakeshore habitats have been rarely explored, and knowledge of the ecological processes associated with their spatial distribution is limited. Here, we employed amplicon‐based high throughput sequencing technology to investigate the composition, co‐occurrence patterns, spatial distribution, and assembly processes of free‐living and particle‐attached bacterial communities (FLBC vs. PABC) across 29 lakeshore zones within freshwater lakes from two typical regions: Middle–Lower Yangtze River Plain and Yunnan–Kweichow Plateau. We found significant community dissimilarity between both bacterial lifestyles and regions. PABC exhibited higher community dissimilarity between the plain and plateau regions than FLBC did. Furthermore, eight operational taxonomic units were detected as a core bacterial community within the lakeshore habitats across the plain and plateau regions. Network analysis revealed that bacterial taxa preferring a particle‐attached lifestyle exhibited more co‐occurrence associations for both intra‐ and inter‐community than the free‐living lifestyle. Both FLBC and PABC showed significant distance–decay relationships in these two regions. Dispersal limitation was the most important process associated with patterns in the spatial distribution of FLBC and PABC, whereas the relative importance of variable and homogeneous selection differed between the assembly of FLBC and PABC because of differences in their microhabitats. Our results highlight that dispersal limitation may be strongly associated with the spatial distribution of lakeshore bacterial communities at a regional scale. However, lifestyle differences influence the relative balance between the processes of dispersal, selection, and biotic interaction that influence these bacterial communities. This study reveals potential interactions between bacterial taxa with different lifestyles as well as indicating which ecological processes may lie behind the spatial distribution of bacterial communities in lakeshore habitats. Knowledge of potential assembly mechanisms of bacterial communities within different microhabitats help us understand how bacterial communities in different microhabitats are generated and their different roles in biogeochemical processes in lakeshore habitats.

Spatial distribution and influencing factors on the variation of bacterial communities in an urban river sediment.

The water and sediments of urban rivers are spatially heterogeneous because of the influence of environmental and anthropogenic factors. However, the spatial and functional diversity of bacterial communities in urban river sediments are unclear. We investigated the spatial distribution of microbial compositions in sediments in Qingdao section of the Dagu River, and the effects of sediment physiochemical properties on the variation were explored. Among the seven heavy metals analyzed, only the average concentration of Cd significantly exceeded the safety limit for sediments. The detailed composition and spatial distribution of bacterial communities fluctuated substantially between sites along the river. Bacterial datasets were separated into three clusters according to the environmental characteristics of sampling areas (the urbanized, scenic, and intertidal zones). For the urbanized zone, Acidobacteria, Firmicutes, Gemmatimonadetes, Bacteroidetes, and Gammaproteobacteria were significantly enriched, implying the effects of human activity. In the intertidal zone, Alphaproteobacteria and Deltaproteobacteria were significantly enriched, which are associated with S redox processes, as in the marine environment. Variation partitioning analysis showed that the amount of variation independently explained by variables of Na, Al, total S and Zn was largest, followed by sediment nutrients, while heavy metals and pH explained independently 13% and 9% of the variance, respectively. Overall, microbial structures in the Dagu River exhibited spatial variation and functional diversity as a result of natural and anthropogenic factors. The results will enable the prediction of the changes in urban river ecosystems that maintain their ecological balance and health.

Bacteria in the lakes of the Tibetan Plateau and polar regions

The Tibetan Plateau, also termed ‘the Third Pole’ harbors the largest number of high-altitude lakes in the world. Due to the presence of extreme conditions such as low temperature and oligotrophy, the lakes of the Tibetan Plateau share environmental features in common with lakes in the polar regions. However, the extent to which these environments are analogous, or indeed whether they harbor similar microbial communities or a high level of endemic species is poorly understood. Here we compared high-throughput 16S rRNA gene sequencing data from the lakes of the three different regions in order to characterize their taxonomic diversity, the community composition and biogeography. Our results showed despite the similarity in environmental conditions, the spatial distribution of the bacterial communities was distinct with only 3.1% of all operational taxonomic units (OTUs) being present in all three regions (although these OTUs did account for a considerable proportion of the total sequences, 36.4%). Sequences belonging to Burkholderiales and Actinomycetales dominated the shared OTUs across all three regions. Scale dependent distance decay patterns provided evidence of dispersal limitation. Climatic variables and dispersal limitation were apparently both important in controlling the spatial distribution of bacterial communities across regions. This work expands our understanding of the diversity and biogeography of lake bacterial communities across the Tibetan Plateau and provides insights into how they compare to those of the Antarctic and Arctic.

Spatial analysis of bacteria in brackish lake sediment

The spatial distribution of bacterial communities inhabiting sediment is heterogeneous at different spatial scales, but mostly unexplored. Here, it is postulated that the heterogeneity of the bacterial community composition varies at the same scale of the heterogeneity of sediment chemical properties. The large spatial scale (km) diversity in sediment from a brackish water lagoon (Chilika Lake, India) is studied, considering the large scale physical and chemical characteristics of land cover, climate, pH, and salinity. Seventy-two samples (24 stations, 3 seasons: winter, rainy, and summer) of sediment from Chilika Lake were analyzed by 16S ribosomal ribonucleic acid (rRNA) gene sequencing, and the relations with land cover and other physico-chemical parameters are discussed. More samples were collected after a severe cyclonic storm (Phailin) passed near the lagoon in 2013, to see the impact of the tropical storm on the spatial and temporal distribution of bacteria in the sediment. The results demonstrate clear spatial relations between physico-chemical parameters (e.g., salinity), land surfaces (e.g., drainage area), and the distribution of sediment microbial communities.

Spatio-temporal Patterns of Microbial Communities and Their Driving Mechanisms in Subalpine Lakes, Ningwu, Shanxi

Human activities and climate change cause the degradation of subalpine lake ecosystems, which induce the shift of microbial community structure. The spatio-temporal dynamics and the diversity maintenance mechanisms of bacterial communities in Gonghai Lake in Ningwu, Shanxi, were investigated by using Q-PCR and DGGE. The results showed that the temperature), pH, dissolved oxygen, electrical conductivity salinity, and ammonium nitrogen (NH4+) contestation were significantly different among the different sampling depths during different months. Bacterial abundance was the highest in August and the lowest in November, and the abundance was higher in the middle water layer (2 m, 4 m, and 6 m depths), but relatively low in the surface layer and bottom layer (0 m and 8 m depths, respectively). The α diversity index of bacterial communities had significant differences among the different months and depths, and showed an initial decreasing trend and then an increasing trend from April to December. A PERMANOVA test showed that the spatial distribution of bacterial communities was significantly different among depths (P<0.001). The results of redundancy analysis and variation partitioning indicated that environmental selection and diffusion limitation had an effect on the maintenance of the diversity patterns of bacterial communities at the different depths of GH. However, the relative effect of environmental factors was stronger, of which the concentration of NO3-, NO2-, and NH4+ were the main influencing factors. In conclusion, the bacterial communities in GH subalpine lake showed clear spatio-temporal distribution patterns, and environmental variables had a significant effect on shaping the community diversity.

Nonferrous metal (loid) s mediate bacterial diversity in an abandoned mine tailing impoundment.

Migration and transformation of toxic metal (loid) s in tailing sites inevitably lead to ecological disturbances and serious threats to the surroundings. However, the horizontal and vertical distribution of bacterial diversity has not been determined in nonferrous metal (loid) tailing ponds, especially in Guangxi China, where the world's largest and potentially most toxic sources of metal (loid) s are located. Distribution of bacterial communities was stable at horizontal levels. At the surface (0-10 cm), the stability was most attributed to Bacillus and Enterococcus, while bacterial communities at the subsurface (50 cm) were mainly contributed by Nitrospira and Sulfuricella. Variable vertical distribution of bacterial communities has led to the occurrence of specific genera and specific predicted functions (such as transcription regulation factors). Sulfurifustis (a S-oxidizing and inorganic carbon fixing bacteria) genera were specific at the surface, whereas Streptococcus-related genera were found at the surface and subsurface, but were more abundant in the latter depth. Physical-chemical parameters, such as pH, TN, and metal (loid) (As, Cd, Pb, Cu, and Zn) concentrations were the main drivers of bacterial community abundance, diversity, composition, and metabolic functions. These results increase our understanding of the physical-chemical effects on the spatial distribution of bacterial communities and provide useful insight for the bioremediation and site management of nonferrous metal (loid) tailings.

Soil bacterial communities exhibit systematic spatial variation with landform across a commercial potato field

Topography drives spatial variation of soil edaphic factors at the landscape scale however, it is unclear how it influences the spatial distribution of bacterial communities in distances relevant to agro-ecosystem management. This study examined the influence of soil physico-chemical properties and topographic features on bacterial communities and diversity in a commercial potato field with a rolling landform. Eighty-three soil samples were systematically collected across a transect 1100 m long. A significant negative correlation (r = −0.73) between soil pH (range 4.3–7.0) and slope gradient (range 1.8–11.9%) was observed. Regressions and/or a canonical correspondence analysis showed that pH, slope gradient and organic carbon were the major factors influencing bacterial α-diversity based on 16S rRNA gene sequences. Semivariogram analyses revealed that the bacterial α-diversity, the relative abundance of most phyla, pH and slope gradient showed strong to medium spatial autocorrelations with a range between 20.8 and 217.8 m. These results evidenced that soil pH and slope gradient were the major factors explaining variation in the spatial structure of the bacterial community. Our results showed that the soil bacterial communities varied in a systematic and predictable pattern in an agricultural field in response to variation in soil physico-chemical properties and topographic features.

Spatial Distribution of Sediment Bacterial Communities in Eutrophicated Meiliang Bay: Correlation with Environmental Factors

Bacterial community structure and the effects of several environmental factors on the spatial distribution of bacterial communities were investigated in the sediment of Meiliang Bay in a large, shallow, eutrophic freshwater lake. Water and surface sediment samples were collected at 10 sampling sites on 15 September 2013. Based on cluster analysis of the DGGE banding patterns, there were no significant variations in spatial distribution of bacterial community structure of the 10 sediment samples in the eutrophicated Meiliang Bay. The dendrogram of the bacterial community similarities in the 10 samples revealed that samples grouped into two defined clusters with a 60% similarity. Analysis of DNA sequences showed that the dominant bacterial groups in the Meiliang Bay belonged to Proteobacteria, Acidobacteria, Cyanobacterium, Planctomycetes, and Verrucomicrobia, which commonly exist in freshwater ecosystems. In addition, some Actinobacteria, Firmicutes, Nitrospirae bacterial groups were also found in the Meiliang Bay. The transformation of nitrogen and phosphorus nutrients in the water and sediments of Meiliang bay influenced on bacterial community in the sediments. Canonical correspondence analysis demonstrated that total nitrogen and total phosphorus in the sediment significantly influenced the bacterial community structure in the sediment of Meiliang Bay.

Bacterial distribution pattern in the surface sediments distinctive among shelf, slope and basin across the western Arctic Ocean

The Arctic Ocean is undergoing a rapid change due to global climate change. Knowledge about bacterial community composition will help us to evaluate their ecological role and predict possible community shifts in future. To this end, we collected 11 sediment samples across the western Arctic Ocean from shelf near to the North Pole, spanning three geographical regions: ice-free Chukchi Shelf, more than 90 % ice-covered Canadian Basin and the partial ice-covered slopes between them. The spatial distribution of bacterial community composition was examined by 16S rRNA gene using Illumina high-throughput sequencing in conjunction with sediment geophysicochemical variables and sampling geographical distance. Overall, Proteobacteria, Acidobacteria, Planctomycetes, Actinobacteria, Chloroflexi, Gemmatimonadetes, Bacteroidetes, Verrucomicrobia, Nitrospira and Thermomicrobia were the top ten dominant phyla. The bacterial communities from the same region exhibited similar composition pattern, but distinctive in each region. Statistical analysis (linear discriminant analysis effect size) showed Deltaproteobacteria, Gammaproteobacteria and Alphaproteobacteria were the distinct taxa in Chukchi Shelf, Slopes and Canadian Basin, respectively. Correspondingly, Desulfobacterales, Planctomycetales and Rhodospirillales were determined at order level. Canonical correspondence analysis demonstrated bacterial distribution was significantly correlated with sediment geophysicochemical factors and geographical distance. Furthermore, variance partitioning analysis confirmed that the combination of geophysicochemical factors, including sediment nutrients content, grain size, sampling site water depth and ice coverage, contributed more than geographical distance to the spatial distribution of bacterial communities across the western Arctic Ocean. In general, this study offers a snapshot of bacterial community composition under the background in rapidly changing Arctic Ocean.