ABSTRACT Yield potential of agricultural fields associated with plant spatial arrangement could determine the physiological quality of soybean (Glycine max L.) seeds. Thus, this study aimed to evaluate the physiological quality of soybean seeds from different yield environments and plant densities. Experiments were carried out in Boa Vista das Missões-RS, Brazil, during the 2014/2015 growing season. Yield environments were delineated by overlapping yield maps from the 2008, 2009/2010 and 2011/2012 growing seasons. The experimental design was a randomized complete block in a 2 x 5 factorial arrangement with two yield environments (low and high) and five plant densities, with four replicates. Two varieties were tested: Brasmax Ativa RR (10, 15, 20, 25 and 30 plants m-1) and Nidera 5909 RR (5, 10, 15, 20 and 25 plants m-1). After harvested, the seeds were analysed as following: first count index, germination, abnormal seedlings, dead seeds, electrical conductivity, accelerate aging test, root length, hypocotyl length and seedling length. The spatial variability of seed vigor in the production field could be reduced by adjusting plant density, but the adjustment should consider the variety. Harvest according to yield environment is a strategy to separate lots of seeds with higher vigor, originated from high-yield environments.