Sparkling Wine Production Research Articles

Exploring the relationship between field available water capacity (AWC) and atypical aging (ATA) development in base wines.

Hydric stress is a leading cause of atypical aging (ATA) in wine, characterized by unpleasant olfactory notes. The main sensorial and chemical marker of ATA is 2-aminoacetophenone (AAP). Early detection of ATA before the second fermentation in sparkling wines (SWs) is crucial for producing high-quality products. Climate change-induced droughts significantly impact agriculture, including grape farming, particularly in vineyards with shallow soils and reduced available water capacity (AWC). This study examined the relationship between AWC and ATA in base wines (BWs) intended for SW production. Ten vineyards were classified into three AWC categories (low, medium, and high). Hydric stress levels were monitored over two growing seasons to explore their effects on vegeto-productive behavior and AAP development. During the first vintage, drought conditions led to potentially ATA-tainted BWs across all AWC classes. The impact varied with AWC, with low-AWC vineyards experiencing higher stress and producing BWs with elevated AAP levels and vegeto-productive imbalance. In contrast, the following season had unusual rainfall, resulting in some potentially ATA-affected BWs, but no significant differences in AAP content or vegeto-productive balance among the AWC classes were observed. In conclusion, grapevines on low-AWC soils are at a higher risk of producing faulty BWs, particularly in dry vintages.

Instrumental and sensory tools to evaluate the production potential of a new type of sparkling wine from umbu (Spondias tuberosa)

Umbu is a native fruit from the Brazilian Caatinga Biome, which is a source of income for families involved in extractivism. This fruit is highly perishable and production losses have been experienced. The production of sparkling wine can be a strategy for better use and to add value to this fruit. Furthermore, consumers have been attracted to innovative beverages, produced with alternative raw materials and with an exotic and pleasant aroma. The potential for producing sparkling wine from umbu was evaluated through physical-chemical, instrumental (gas chromatography with detection by mass spectrometry and olfactometry) and sensory analyses (acceptance test, Just-about-right (JAR) scale, and penalty analysis). Both the base wine and the sparkling wine meet all regulatory physicochemical parameters. The volatile profile of umbu pulp contains mainly terpenes (55%), while esters predominated in the base and sparkling wines (32 and 33%, respectively). In sparkling wines, hexanoic acid (3114 mg L−1) and linalool (1266 mg L−1) were the major compounds. Among the 26 odor-active compounds of sparkling wines are mainly ethyl esters with fruity aroma (acetate, decanoate, and 2-phenylacetate; OSME area: 4.8, 4.8, and 5.0%, respectively), and terpenes with floral notes (p-cymene, geranic oxide and linalool; OSME area: 8.5, 7.4, and 6.9%, respectively). The acceptance and purchase intention tests endorsed the commercialization expectation, although JAR scale and penalty analysis showed that the umbu aroma and sweetness may be improved. The potential of umbu for the production of sparkling wines has been proven, which satisfies the current market demand for innovative beverages.

Testing of ultrasonic vibration to speed up the remuage operation in sparkling wine production

The research aims at providing an innovative method for remuage, testing the use of ultrasonic vibrations induced directly into the bottles to accelerate the process. This ultrasonic technique generates vibrations that cause the lees to move more swiftly along the slope of the tilted bottle, leading to a quicker accumulation of lees in the bottleneck. The efficacy of the method was measured using also image analysis and the effects on the wine were evaluated using sensory analysis. The implementation of the tested system showed significant time savings during the remuage process (2.5 h for the remuage process), without negatively impacting the sensory qualities of the wine produced (no statistically significant differences at 99.9% compared to traditional remuage methods). Moreover, the manuscript discussed the internal strengths and weaknesses, along with external opportunities and threats, that the research outputs encounter while the potential implementation of the prototype for ultrasonic remuage. In the future, a remuage prototype can be envisaged that can handle multiple batches of bottles, such as a conversion kit for existing riddling machines. This would provide a cost-effective and efficient solution for wineries looking to optimize their sparkling wine production.

Исследование качества молодых игристых вин из крымских автохтонных сортов винограда

Most domestic sparkling wines are made of traditional champagne grape varieties. However, indigenous Crimean cultivars could increase the output of high-quality original beverages if a proper technology was introduced. 
 This research featured young sparkling wines from Crimean grape varieties, i.e., Soldaya, Shabash, Kokur Beliy, Sary Pandas, Kefesiya, Dzhevat Kara, and Ekim Kara. Organic acids, sugars, glycerin, phenolic substances, and ethanol were determined by high performance liquid chromatography; the content of amine nitrogen was measured by formalin titration. The optical profile of the wine samples was subjected to the colorimetric method while their foamy properties were studied by bubbling the samples in a measuring cylinder. The sparkling properties depended on the rate of CO2 desorption; the volumetric method made it possible to define the CO2 content; the viscosity was measured using a viscometer. The sensory assessment followed State Standard 32051-2013, ISO 5492:2008, and ISO 11035:1994. 
 The samples of Kokur Beliy, Sary Pandas, and Soldaya received high tasting ratings (≥ 9.0 points), as did the red variety of Kefesiya (8.95 points). They demonstrated a clear typical aroma and a harmonious taste, as well as good foamy and sparkling properties. The maximal foam volume correlated with amine nitrogen (r = 0.762) while the sparkling properties correlated with the mass fraction of bound CO2 (r = 0.977). The red sparkling wine from the Kefesiya variety had a dark garnet color due to its high anthocyanins. It also contained quercetin and glycoside, which are known for their biological (P-vitamin) activity and powerful antioxidant properties. The early low-sugar (< 18 g/100 cm3) varieties of Dzhevat Kara and Ekim Kara had the same acidity but demonstrated a less diverse phenolic profile and were paler in color. 
 In this research, the indigenous Crimean grape cultivars of Kokur Beliy, Sary Pandas, Soldaya, and Kefesia showed good prospects for young sparkling wines. Other varieties needed special technologies to preserve their typical properties and the aroma/taste balance. The new technology makes it possible to obtain unique high-quality products right in the harvest year, thus increasing the range and volume of sparkling wine production.

Evaluating the Atypical Aging Potential Development in Sparkling Wines Can Be Achieved by Assessing the Base Wines at the End of the Alcoholic Fermentation.

Traditional sparkling wine production is a lengthy and costly process, involving a double fermentation step and a period of aging sur lie; thus, monitoring quality during the key manufacturing stages is crucial. The effects of the second fermentation on the development of 2-aminoacetophenone (AAP), the main marker of the atypical aging (ATA) defect, were investigated on 55 base wines (BWs) and corresponding sparkling wines (SWs) produced in an experimental winery. While the AAP content of the SWs was observed to be higher than the BWs, it was found that an artificial aging test carried out on the BWs could be a good predictor of ATA development in SWs. Further, the antioxidant capacity of the SWs was noticed to correlate well with the potential AAP formed during accelerated aging. Finally, an analysis of covariance (ANCOVA) model of linearization capable of predicting AAP formation in SWs using the data obtained from the corresponding BWs was created.

Can Italian wines outperform European benchmarks in environmental impact? An examination through the product environmental footprint method

Nowadays, there is a pressing demand for precise tools to quantify sustainability and assess the contributions of products and processes to sustainable development. This requirement extends to the wine industry as well. In 2013, the European Commission introduced the Product Environmental Footprint (PEF), providing a standardized methodology grounded in life cycle thinking for evaluating the environmental impacts of products across various industries. Despite its potential and the availability of specific guidelines for wine, the application of PEF remains unexplored in the sector. This study contributes to the knowledge by applying PEF to assess environmental impacts of Italian still and sparkling wines production, identifying variations from European benchmarks. Additionally, it aims to pinpoint pivotal hotspots and provide guidance for effective mitigation strategies.Average data from 27 wines certified as sustainable under Italian VIVA program, were collected and used to perform a life cycle assessment in accordance with PEF protocol. Results revealed that Italian still wines exhibited a greater environmental impact than the European reference value, while sparkling wines displayed a slightly more favourable environmental performance compared to the European average. Notably, specific impact categories remained consistent across different wines and countries, with climate change, resource utilization, land use, and particulate matter harmful for human health accounting for nearly 80 % of the overall environmental footprint. Hotspot analysis identified the plantation/destruction of vines, energy usage in the winery, and packaging as significant factors influencing the environmental footprint of Italian wines. Addressing these elements could enhance the environmental competitiveness of the Italian wine sector relative to its European counterparts. However, to validate these findings, further studies are necessary, both within Italy and in other European wine-producing regions. Such research initiatives will improve and strengthen PEF methodology, bolstering its adoption as the primary tool for environmental impact assessment of wine at the community level.

СОВРЕМЕННЫЕ ЗАДАЧИ НАУЧНЫХ ИССЛЕДОВАНИЙ В ОБЛАСТИ ВИНОДЕЛИЯ

The article presents the results of research activities for 2023 of the Federal State Budget Scientific Institution All-Russian National Research Institute of Viticulture and Winemaking Magarach of the RAS in the field of winemaking, carried out in accordance with the Program of Fundamental Scientific Research in the Russian Federation for the long term (2021-2030). The methodology of wine quality management with ecostatus has been created, aimed at revealing the individual characteristics of wines caused by natural factors and reducing the risks of negative effects on humans. Promising yeast strains have been identified: Steinberg, Champanskaya G-14, ShM-30, Novocimlyanskaya 3 with a description of their taxonomic affiliation, morphological and physiological-biochemical properties. Two strains of O. oeni active acid-reducing agents are recommended for practical use. The application of technology for the production of young sparkling wines of Crimean autochthonous grape The article presents the results of research activities for 2023 of the Federal State Budget Scientific Institution All-Russian National Research Institute of Viticulture and Winemaking Magarach of the RAS in the field of winemaking, carried out in accordance with the Program of Fundamental Scientific Research in the Russian Federation for the long term (2021-2030). The methodology of wine quality management with ecostatus has been created, aimed at revealing the individual characteristics of wines caused by natural factors and reducing the risks of negative effects on humans. Promising yeast strains have been identified: Steinberg, Champanskaya G-14, ShM-30, Novocimlyanskaya 3 with a description of their taxonomic affiliation, morphological and physiological-biochemical properties. Two strains of O. oeni active acid-reducing agents are recommended for practical use. The application of technology for the production of young sparkling wines of Crimean autochthonous grape varieties: Kokur belyi, Sary Pandas, Soldaiya and Kefesiya is justified. The system of criteria indicators for assessing the quality of grapes for the production of high-quality cognac distillates and cognacs is substantiated. A working collection of 63 natural strains of lactic acid bacteria has been formed. The optimal technological factors of the process of single-stage extraction by infusing with a food water-alcohol solution of green grape shoots of the Vitis vinifera, Vitis abrusca species have been established, allowing to increase the yield of total polyphenols by at least 2.6 times for the production of functional products. The most optimal technological modes of cold treatment of dry and fortified wine materials have been established to substantiate the constructive solution of an energy-saving installation for the purpose of cold treatment of wine materials based on the study of the main factors affecting the mass transfer process of potassium bitartrate crystallization. The obtained results of research work contribute to improving the quality of domestic wine products and expanding its production, including winemaking with ecological and geographical statuses, the production of domestic cognac and grape processing products of a functional orientation. The introduction of scientific developments into production will increase the competitiveness of domestic wine products, accelerate import substitution and ensure the country's food independence.

The Importance of Region of Origin in Sparkling Wines: An International Analysis of Consumers’ Perception

Consumers’ perception of the region of origin represents a critical issue for building a valuable and sustainable business in the wine sector. The aim of this study is to investigate the role of the region of origin in Italian sparkling wines, comparing consumers’ perception of emerging wines with a designation of origin in regions not traditionally associated with sparkling wine production (Abruzzo and Romagna), with an affirmed brand (Prosecco) and with generic Italian sparkling wine. The three most important markets for Italian sparkling wines (Italy, the USA, and the UK) are considered. The importance of the region of origin is analyzed within a set of wine attributes, including color and sugar content, applying the conjoint analysis technique to simulate realistic market conditions, offering multiple options for wine attributes. There is no cumulative effect of the perception of wines with a Protected Designation of Origin in the three markets since consumers who positively perceive a specific designation do not extend this positive perception to others in comparison with generic sparkling wine. Positive and negative utilities associated with each designation of origin are calculated and used to discriminate consumers according to socio-demographic variables, consumers’ behavior, and attitudes.

Role of amino acids in flavor profiles and foam characteristics of sparkling wines during aging

As sparkling wine ages, a well-balanced amino acid composition can enrich its flavor profiles and achieve an optimal state for foam characteristics. This study aimed to determine the influence of amino acids on the flavor profiles and foam characteristics of white and rosé sparkling wine. The concentrations of amino acids and aroma compounds were determined via liquid chromatography–mass spectrometry (LC–MS) and gas chromatography-mass spectrometry (GC-MS), respectively. Correlation analysis and multiple linear regression (MLR) models were employed for further analysis. It revealed that the content of amino acids (except for proline) in both sparkling wines decreased with aging (11–23%). Ethyl octanoate had the largest odor activity value (OAV) value in both wines, but its content decreased with aging. The foam parameters of both sparkling wines decreased with increased aging duration, leading to changes in effervescence-related taste traits. Correlation analysis showed that most amino acids positively correlated with ethyl octanoate and foam characteristics, whereas the foam parameters did not increase along with the protein concentrations. Furthermore, the MLR analysis emphasized that amino acids had a greater impact on foam characteristics than volatiles. This study provides guidance for the production of high-quality sparkling wines.

ВЛИЯНИЕ СОРТОВОГО ФАКТОРА НА ФОРМИРОВАНИЕ ПЕНООБРАЗУЮЩЕЙ СПОСОБНОСТИ ВИНОМАТЕРИАЛОВ

Currently, the production of high-quality sparkling wines is one of the main tasks in wine-making. The indicator of foamy and sparkling properties of finished sparkling wines directly depends on the ability of the base wine to foam. This indicator is influenced by various factors: the grape variety, the composition of the wine material, the agrotechnological and technological methods used. The main purpose of the study was to study the influence of varietal characteristics of grapes on the foaming ability of wine materials produced in the Krasnodar region in the 2022 season. All the original samples were processed and fermented under identical conditions. The study of the component composition of the samples was carried out according to standard methods using modern equipment. To determine the ability of foam formation by the initial samples, a "Foaming analyzer" was used, developed by Mishin M.V. on the basis of the Department of Technology of Wine-making and Fermentation named after Professor A.A. Merzhanian, which allows an objective assessment of the quality of foam. The foaming ability of three introduced grape varieties was analyzed: Pinot Blanc, Riesling Rhenish and Chardonnay. The result of the study was the establishment of a connection between the foaming ability of wine materials and the grape variety. In the course of the study, it was found that all the samples under study, both in terms of chemical composition and organoleptic evaluation of the aroma, were suitable for creating sparkling wine. However, according to the totality of the composition (esters, higher alcohols and amino acids), wine materials from the Riesling Rhenish variety had the greatest potential for the formation of foaming capacity for the 2022 season in the Temryuk district of the Krasnodar region.

Addition of Organic Acids to Base Wines: Impacts on the Technological Characteristics and the Foam Quality of Sparkling Wines.

Climate change is causing a significant decrease in the total acidity of grapes and related wines. This represents a serious issue for sparkling wine production. Consequently, before the second fermentation, the acidification of base wines is often necessary. However, the impacts of the most important organic acids on the foam properties of sparkling wines are not yet well known. The impacts of the addition of tartaric, malic, citric, and lactic acid on the quality of Falanghina and Bombino sparkling wines were evaluated. Analyses were performed soon after the second fermentation and one year after aging sur lees. The addition of each different organic acid to the two base wines resulted in significant changes in the sparkling wines not only in terms of pH, titratable acidity, and buffering capacity but also in the content of total amino acids and, in some cases, in the height of the foam and its stability over time. For both grape varieties, acidified wines showed a lower content of total amino acids in comparison with the control wines. The addition of lactic acid determined a higher persistency of the foam even after one year of aging sur lees only in Falanghina wines. The results obtained herein highlight the importance of organic acids and the pH of the base wines for the content of amino acids in sparkling wines. No strict correlation between organic acid addition and the foamability of wines was observed.

Innovations in Sparkling Wine Production: A Review on the Sensory Aspects and the Consumer’s Point of View

Sparkling wines have a relevant economic value, and they are mostly produced worldwide with the Traditional method (in bottles) or with the Charmat method (in autoclaves). Many varieties are employed in different viticultural areas to obtain white or rosé wines and red (Italy and Australia), with different sugar content. This review illustrates the most recent studies (last 5 years) on sparkling wines concerning innovative yeasts, aromatic profile, aging on lees, sugar types, base wine, new varieties, and innovative oenological techniques, which consider the effects on the sensory characteristics and the consumer preferences.

ТЕХНОЛОГИЧЕСКАЯ ОЦЕНКА СОРТОВ ВИНОГРАДА АНТЕЙ МАГАРАЧСКИЙ И АЙ-ПЕТРИ СЕЛЕКЦИИ ИНСТИТУТА «МАГАРАЧ»

The article discusses the physicochemical parameters and biochemical properties of Antey Magarachsky and Ai-Petri grape varieties for the production of pink wine materials. The technological features of grapes, consisting in the extracting ability of phenolic, including dyes substances, depending on the methods of its processing. According to the total accounting of the indicators of the carbohydrate-acid complex of the must, including the mass concentrations of sugars and titratable acids, glucoacidimetric and technical maturity index, it is promising to use the variety Antey Magarachsky (technical maturity index - 182, glucoacidimetric index - 2,8) for the production of pink sparkling wines. For the possible use of the Ai-Petri grape variety, the control and regulation of the carbohydrate-acid complex is required. The indicators of the phenolic complex of the breeding grape varieties exceeded the values of the control variety, which leads to control at the stage of grape processing to obtain high-quality pink wine materials. The conclusion is made about the expediency of their use in the production of pink table wine materials.

Insights into the grape must composition effect on Hanseniaspora vineae performance and metabolic aroma compounds in Chardonnay base wine for sparkling wine production

This study compares the performance of Hanseniaspora vineae (Hv) under mixed culture fermentation with Saccharomyces cerevisiae, with a conventional control (Sc) for the production of base wine. Hv exhibited fermentation kinetics comparable to controls when its nutritional requirements were satisfied. Volatile acidity in Hv from one batch was significantly lower (–0.3 g/L) compared to the controls, while in the other two batches the levels were equal. Interestingly, Hv trials showed a significant degradation of malic acid (–0.9 g/L on average) without variations of pH. Even though the matrix influenced the volatile composition, the characteristic features of H. vineae remained intact, with 24-fold higher levels of 2-phenylethyl acetate. There was no higher production of 2-aminoacetophenone, albeit tryptophan (known as a 2-aminoacetophenone precursor) is a preferred amino acid for H. vineae. Results suggest the potential of H. vineae to produce base wine with a broad spectrum of aroma compounds that increase flavor complexity, if obtained from nutrient-balanced grape musts.

Endogenous CO2 Overpressure Effect on Higher Alcohols Metabolism during Sparkling Wine Production.

Higher alcohols produced by yeast during the fermentation of sparkling wine must have the greatest impact on the smell and taste of wine. At present, the metabolic response to methanol and higher alcohols formation of Saccharomyces cerevisiae under endogenous CO2 overpressure has not been fully elucidated. In this work, a proteomics and metabolomics approach using a OFFGEL fractionator and the LTQ Orbitrap for the protein identification, followed by a metabolomic study for the detection and quantification of both higher alcohols (GC-FID and SBSE-TD-GC-MS) and amino acids (HPLC), was carried out to investigate the proteomic and metabolomic changes of S. cerevisiae in relation to higher alcohols formation under a CO2 overpressure condition in a closed bottle. The control condition was without CO2 overpressure in an open bottle. Methanol and six higher alcohols were detected in both conditions, and we have been able to relate to a total of 22 proteins: 15 proteins in the CO2 overpressure condition and 22 proteins in the control condition. As for the precursors of higher alcohols, 18 amino acids were identified in both conditions. The metabolic and proteomic profiles obtained in both conditions were different, so CO2 overpressure could be affecting the metabolism of higher alcohols. Furthermore, it was not possible to establish direct correlations in the condition under CO2 overpressure; however, in the condition without pressure it was possible to establish relationships. The data presented here can be considered as a platform that serves as a basis for the S. cerevisiae metabolome-proteome with the aim of understanding the behavior of yeast under conditions of second fermentation in the production of sparkling wines.

Impact of meteorological conditions, canopy shading and leaf removal on yield, must quality, and norisoprenoid compounds content in Franciacorta sparkling wine.

Climate change is a major concern in agriculture; in grapevine production, climate change can affect yield and wine quality as they depend on the complex interactions between weather, plant material, and viticultural techniques. Wine characteristics are strongly influenced by microclimate of the canopy affecting primary and secondary metabolites of the grapevine. Air temperature and water availability can influence sugar and acid concentration in grapes and relative wines, and their content of volatile compounds such as norisoprenoids. This becomes relevant in sparkling wine production where grapes are generally harvested at a relatively low pH, high acidity, and low sugar content and where the norisoprenoids significantly contributes to the final aroma of the wine. The effect of climate change on grapevine and wine, therefore, calls for the implementation of on-field adaptation strategies. Among them canopy management through leaf removal and shading have been largely investigated in the wine growing sector. The present study, conducted over 4 years (2010-2013) aims at investigating how leaf removal and artificial shading strategies affect grape maturation, must quality and the production of norisoprenoids, analyzed using an untargeted approach, in sparkling wine. Specifically, this paper investigates the effect of meteorological conditions (i.e., water availability and temperatures) and the effect of leaf removal and shading on Vitis vinifera L. cv. Chardonnay and Pinot noir, which are suitable to produce sparkling wine in the DOCG Franciacorta wine growing area (Lombardy, Italy). The effect of leaf removal and shading practices on norisoprenoids has been the focus of the study. No defoliation and artificial shading treatments play an important role in the preservation of the acidity in warm seasons and this suggests calibrating defoliation activities in relation to the meteorological trend without standardized procedures. This is particularly relevant in the case of sparkling wine, where the acidity is essential to determine wine quality. The enhanced norisoprenoid aromas obtained with a total defoliation represent a further element to direct defoliation and shading strategies. The obtained results increase knowledge about the effect of different defoliation and artificial shading applications in relation to meteorological condition supporting the management decision-making in the Franciacorta wine growing area.

Characterization of Sparkling Wine Based on Polyphenolic Profiling by Liquid Chromatography Coupled to Mass Spectrometry

Polyphenols are phytochemicals naturally present in wines that arouse much interest in the scientific community due to their healthy properties. In addition, their role as descriptors of various wine qualities, such as the geographical origin or the grape variety, cannot be underestimated. Here, Pinot Noir and Xarel·lo monovarietal samples belonging to the sparkling wine production process have been studied, corresponding to base wines from a first alcoholic fermentation (plus malolactic in some cases), base wines resulting from tartaric stabilization, and sparkling wines from a second alcoholic fermentation aged for 3 and 7 months. One of the objectives of this paper is to obtain valuable chemical and oenological information by processing a huge amount of data with suitable chemometric methods. High-performance liquid chromatography coupled with ultraviolet spectroscopy and tandem mass spectrometry (HPLC-UV-MS/MS) has been used for the determination of polyphenols in wines and related samples. The method relies on reversed-phase mode and further detection by multiple reaction monitoring. Concentrations of relevant phenolic compounds have been determined, and the resulting compositional data have been used for characterization purposes. Exploratory studies by principal component analysis have shown that samples can be discriminated according to varietal and quality issues. Further classification models have been established to assign unknown samples to their corresponding classes. For this purpose, a sequential classification tree has been designed involving both variety and quality classes, and an excellent classification rate has been achieved.

Influence of Expeditionary Liqueur on The Formation of Foam Properties of Sparkling Wines

When determining the quality of sparkling wines, first of all, we thought to focus on the assessment of processes of formation of sparkling and frothy properties, as the process of cavitation reveals the relationship with the chemical components, which affects organoleptic indicators. The effect of expeditionary liquor on frothy properties was investigated and it was found that expeditionary liquor directly affects the concentration of dissolved carbon dioxide. The control samples were made according to the classical technology of sparkling wine production, including “prise de mousse”, due to which the carbon dioxide molecules were promoted and dissolved, increasing the degree of diffusion in the liquid. Such a sparkling wine is characterized by a fine foam and a highpressure index. Foam formation is associated with proteins, as they exhibit the ability to adsorb and unfold at the gas-liquid interface, and foam stability is provided by amino acids due to strong bonds that increase wine viscosity. When expeditionary liqueur was added, the pressure dropped from 7 atmospheres to 4 atmospheres, which allowed the sparkling wine to calm down and stabilize bubble formation, and this is the result of the subtle interaction between dissolved carbon dioxide molecules and tensioactive wine components.

Special characteristics of technological indicators of white aboriginal grape varieties

Sparkling wines are high in demand, and they firmly occupy their strong position in wine market. In recent years, there was a reorientation of consumers from the low-price segment of sparkling wines to the mid-price and premium ones, motivating the manufacturer to be searching for new solutions for the unique high-quality wine production. One of the ways of its producing is the use of aboriginal grape varieties. At the same time, there is insufficient information about the distinctive technological characteristics of aboriginal grape varieties, which makes it difficult to release a product of high-quality. As a research result, significant indicators were established (the mass concentrations of titratable acids, proteins, polysaccharides, the content of phenolic substances in the must without contact with the pulp, the content of phenolic substances in the must after extraction of the pulp for 4 hours, the technological stock of phenolic substances) to differentiate grapes in accordance with the origin, and characterize their technological potential. A comparative analysis of aboriginal grape varieties with classical cultivars traditionally used in sparkling wine production was carried out. Deviations in the criterion technological indicators of aboriginal grape varieties were established to be taken into account when selecting grape processing technology for high-quality sparkling wine production.

Yeast Biomass Use Optimization in the Wine Champanization Unit Biogenerator

The yeast biomass use optimization in continuous wine champagnization plants has been the research and development subject for many years aimed at improving processes and devices in the sparkling wines production. At the same time, a man pays careful attention to the possibility of enriching the champagnized wine with biologically active substances and surfactants generated by yeast. The work aim is to increase the process efficiency of enriching champagnized wine with biologically active substances and surfactants. The development subject is a new technology for the rational yeast biomass use, involving submitted recirculation circuit introduction with the yeast cells separation and their return to the technological cycle without stopping the flow; as well as design modernization of the biogenerator apparatus and membrane microfiltration module calculation. A man suggested to divide the biogenerator apparatus into four sections equal in volume by transverse perforated partitions made of flat steel sectors, laid on knife-edge, filled with attachments: in the first section there is a regular packing with a corrugated profile and a channel width of 50 mm, in the second section – an irregular dump packing of the Lessing rings 30×30 mm type, in the third and fourth sections there are similar packings with 20×20 mm and 10×10 mm feature sizes, respectively. As a result of the improved technology, the product is enriched with biologically active substances and surfactants. An increase in the number of cells released from the layers adhered to the sorbent intensifies the mass exchange processes between yeast and wine material. Tests of the upgraded biogenerator in production conditions demonstrated that the single fully autolysated cells yield increased by 2.7 times (from 0.2·106 to 0.55·106 cells/ cm3 ), which indicated a positive mass transfer dynamic by biologically active substances and surfactants cells, and consequently, the process effectiveness aimed at the finished products quality improvement.