Castanea Sativa Research Articles

85 years counteracting an invasion: chestnut ecosystems and landscapes survival against ink disease

A retrospective examination of early pest and disease outbreaks, such as ink disease, offers new insights into their impact on ecosystems and landscapes. Ink disease, caused by Phytophthora spp., is one of the most destructive diseases affecting Castanea sativa Mill. It first appeared in Europe in the early 18th century, with the initial recorded case in Italy (Piedmont) dating back to 1845. However, its spread instilled significant concerns in several chestnut-growing regions primarily devoted to fruit production. In 1938, a comprehensive assessment of Phytophthora xcambivora outbreaks was conducted in the Bolognese Apennines (Italy), providing a detailed overview of chestnut cultivation status. Thirty-six disease foci were documented, and laboratory tests confirmed the presence of ink disease. To understand the disease’s impact on chestnut ecosystem and landscape over the past 85 years, the 1938 sites were revisited to assess chestnut persistence and phytosanitary status, with Phytophthora species detected using isolation and molecular techniques. Monitoring data revealed that C. sativa still thrives in all but one site, with its wood seemingly able to coexist in dynamic equilibrium with the disease. While P. xcambivora was still detected in several foci, the extent of damage appeared limited. The potential for natural control, likely influenced by the complexity of soil biota, along with the natural spread of hypovirulence in chestnut blight and biological control of the Asian chestnut gall wasp, could explain the continued presence of chestnut in the investigated area and potentially throughout the Italian chestnut range, despite nearly two centuries of ink disease prevalence. Nevertheless, ongoing monitoring is essential to dynamically comprehend the factors at play and their efficacy, particularly in the context of climate change and the possible spread of other Phytophthora species. The survival of chestnut ecosystems amidst ink disease underscores the preservation of the economic, ecological, and landscape values associated with these woodlands.

Effect of climate and wood type on elastic modulus of heat-treated wood and its optimization by the Taguchi method

Wood, as the oldest building material, provides some of the basic needs of human beings, including shelter and protection. Wood is used in exterior cladding, carrier systems, joinery, ceiling-floor coverings, windows, doors, and furniture production. When wooden material is exposed to external weather conditions, due to its hygroscopic structure, its physical and mechanical properties deteriorate from exposure to moisture, temperature, and biological organisms. The bending modulus of elasticity of Scots pine (Pinus sylvestris L.), oak (Quercus petraea L.), and chestnut (Castanea sativa M.) wood that was tannin-impregnated and heat-treated at 160 °C, was investigated using Taguchi L9 (33). The sequence was optimized. After heat treatment, the carrier elements were subjected to artificial climate conditions. In the optimization of the data obtained, it was understood that the highest impact factor was the tree type. In contrast, the climate on the elastic modulus was the lowest impact factor. In Taguchi analysis, a mathematical prediction model was created using actual and predicted data using the S/N ratio’s biggest-best equation. The R2 of the model can be predicted with an accuracy rate of 98.6%.

First description of adenosine production by Gnomoniopsis smithogilvyi, causal agent of chestnut brown rot

Gnomoniopsis smithogilvyi (Gnomoniaceae, Diaporthales) is the main causal agent of chestnut brown rot on sweet chestnut worldwide. The rotting of nuts leads to alterations in the organoleptic qualities and decreased fruit production, resulting in significant economic losses. In 2021, there was an important outbreak of chestnut rot in southern Galicia (Spanish northwest). The profile of secondary metabolites from G. smithogilvyi was studied, especially to determine its capability for producing mycotoxins, as happens with other rotting fungi, due to the possible consequences on the safety of chestnut consumption. Secondary metabolites produced by isolates of G. smithogilvyi growing in potato dextrose agar (PDA) medium were identified using liquid chromatography coupled with high-resolution mass spectrometry. Three metabolites with interesting pharmacological and phyto-toxicological properties were identified based on their exact mass and fragmentation patterns, namely adenosine, oxasetin, and phytosphingosine. The capacity of G. smithogilvyi to produce adenosine in PDA cultures was assessed, finding concentrations ranging from 176 to 834 µg/kg. Similarly, the production of mycotoxins was ruled out, indicating that the consumption of chestnuts with necrotic lesions does not pose a health risk to the consumer in terms of mycotoxins.

Beneficial Effects of Castanea sativa Wood Extract on the Human Body and Possible Food and Pharmaceutical Applications.

The aim of this review was to investigate the potential use of Castanea sativa wood extract as a food supplement and to evaluate its beneficial properties for human health. The results of the limited amount of studies suggest promising properties, including potential anti-inflammatory effects. The literature indicates that the extract, which is rich in bioactive compounds such as tannins, offers promising therapeutic possibilities for the treatment of conditions associated with chronic inflammation. Consequently, interest in its use in food and pharmaceuticals is growing. Phytochemical studies have reported antioxidant and antimicrobial activities, and anti-inflammatory, anticancer, hypolipidemic, hypoglycemic, and neuroprotective activities. A suitable extraction method and solvent is crucial for the isolation of bioactive compounds, being green extraction technologies outstanding for the industrial recovery of chestnut wood's bioactive compounds. Nevertheless, it is important to emphasize the importance of adhering to regulatory guidelines and obtaining the necessary approvals from regulatory authorities to ensure product safety and compliance. The regulation of herbal medicinal products with proven efficacy and traditional herbal medicinal products is well defined, monitored by authorized bodies, and subject to strict control measures. It is noteworthy that medicinal products are subject to stringent quality testing to ensure safety and efficacy in use, whereas there are no comparable regulatory standards and specific labeling requirements for dietary supplements. When using herbal products, compliance with established standards in health research is essential.

Characterizing the antioxidant activity and sensory profile of chestnuts (Castanea sativa Mill.) grown in the Cimini Mountains of central Italy

For the first time, the antioxidant activity, sensory profile and fatty acid composition of an exceptional chestnut cultivar from the Cimini Mountains in central Italy have been studied in order to evaluate the possibility of adding value to this product, both for its excellent taste and as a source of bioactive compounds. Its main characteristic is its complex aroma. The chestnuts are characterized by the presence of almond (7.5) and floral (7) aromas. The high scores in the attributes of flavour (8), crispness (8) and sweetness (7) are worthy of note. The saturated fatty acid fraction was low in Vallerano chestnuts, both with and without pellicle (28% and 27.8% respectively). On the other hand, the percentage of unsaturated fatty acids was high (from 72% to 72.2%). The lipid fraction of chestnuts was mainly composed of linoleic, oleic and palmitic acids. The samples showed high antioxidant capacity values of 376.99 μmol TE/g (ABST) and 0.64 mmol Fe2+E/g (FRAP), making them nutritionally valuable. For their high quality and organoleptic features, chestnuts in the area of the Protected Denomination of Origin “Vallerano Chestnut” could be comparable to Marrone chestnuts.

A Handheld Laser-Scanning-Based Methodology for Monitoring Tree Growth in Chestnut Orchards.

Chestnut and chestnut byproducts are of worldwide interest, so there is a constant need to develop faster and more accurate monitoring techniques. Recent advances in simultaneous localization and mapping (SLAM) algorithms and user accessibility have led to increased use of handheld mobile laser scanning (HHLS) in precision agriculture. We propose a tree growth monitoring methodology, based on HHLS point cloud processing, that calculates the length of branches through spatial discretization of the point cloud for each tree. The methodology was tested by comparing two point clouds collected almost simultaneously for each of a set of sweet chestnut trees. The results obtained indicated that our HHLS method was reliable and accurate in efficiently monitoring sweet chestnut tree growth. The same methodology was used to calculate the growth of the same set of trees over 37 weeks (from spring to winter). Differences in week 0 and week 37 scans showed an approximate mean growth of 0.22 m, with a standard deviation of around 0.16 m reflecting heterogeneous tree growth.

Significance of Gnomoniopsis smithogilvyi as kernel rot of sweet chestnut in Turkey

AbstractChestnut nut rots caused by fungi, especially by Gnomoniopsis smithogilvyi, showed a significant increase not only in Turkey but also in the other chestnut‐producing countries. In this study, the prevalence of chestnut kernel rot was determined by using 800 fruits collected from eight significant chestnut‐growing provinces of Turkey located in the Black Sea, Marmara, and Aegean Regions. Six types of kernel rot symptoms were determined from the necrotic nuts, brown rot being the most common accounting for 62% of the necrotic nuts. Along with G. smithogilvyi, seven additional fungal species Colletotrichum fioriniae, C. godetiae, Cryphonectria parasitica, Diplodina agrifoliae, Fusarium concentricum, Fusarium tricinctum, and Trichothecium roseum were found to be associated with kernel rot. In addition, some other ubiquitous fungi such as Penicillium spp. were detected. All the pathogenic fungi were identified through a combination of morphological traits and phylogenetic analysis involving partial internal transcribed spacer (ITS) sequences, as well as tef1 and tub2 loci sequences. The most frequently isolated fungus was G. smithogilvyi, and it was found at 98, 31, 10, and 1 of the necrotic kernels out of 291 showing brown rot, brown to black rot, cream colour decay, and white rot symptoms, respectively. It was not found from the kernels showing green rot and greenly‐blue rot symptoms. G. smithogilvyi was prevalent in six of eight chestnut‐growing locations in Turkey. Based on the findings, G. smithogilvyi was shown to be the main fungal agent of brown kernel rot of chestnuts, and some of the other fungi obtained from the kernels were shown to contribute kernel rots of chestnuts.

Forecasting of Airborne Conidia Quantities and Potential Insect Associations of Cryphonectria parasitica, the Causal Agent of Chestnut Blight, in England.

Sweet chestnut, an Asiatic tree introduced in many parts of Europe including the United Kingdom, is planted for nut production, timber, and amenity. Its major threat is the disease called blight, caused by the fungus Cryphonectria parasitica, which infects through wounds by airborne spores. Field trapping using sticky rods rotating traps was performed in an infected area in Devon (between May 2021 and April 2023). An improved dual hydrolysis Taqman probes real-time PCR was used. The number of spores was calculated by comparing the cycle threshold to the Ct of standards with known amounts of conidia or known target fragment copies cloned into a plasmid. Weekly spore counts were in the range of around 60 to approximately 8.5 × 103, with fluctuations of peaks (mainly in late summer-autumn 2021) and troughs. The effects of weather parameters were modelled, finding correlations between spore numbers and temperature, humidity, dewpoint, rainfall, wind speed, and wind duration. Additionally, an insect trapping was performed to confirm the presence/absence and quantity of C. parasitica conidia potentially phoretic on some insects by using the same molecular approach. None of the ten collected insect species harboured spores of this fungus.

Trunk Injection Delivery of Biocontrol Strains of Trichoderma spp. Effectively Suppresses Nut Rot by Gnomoniopsis castaneae in Chestnut (Castanea sativa Mill.).

Gnomoniopsis castaneae is responsible for brown or chalky nut rot in sweet chestnut (Castanea sativa), causing heavy reductions in nut production. Controlling it is challenging, due to its inconspicuous infections, erratic colonization of host tissues and endophytic lifestyle. Fungicides are not applicable because they are prohibited in chestnut forests and strongly discouraged in fruit chestnut groves. Trichoderma species are safe and wide-spectrum biocontrol agents (BCAs), with a variety of beneficial effects in plant protection. This study tested selected strains of T. viride, T. harzianum and T. atroviride for their ability to suppress G. castaneae. Field experiments were conducted in four chestnut groves (two test plots plus two controls) at two sites with a different microclimate. As the size of the trees were a major drawback for uniform and effective treatments, the Trichoderma strains were delivered directly by trunk injection, using the BITE® (Blade for Infusion in TrEes) endotherapic tool. The BCA application, repeated twice in two subsequent years, significantly reduced nut rot incidence, with a more marked, presumably cumulative, effect in the second year. Our data showed the tested Trichoderma strains retain great potential for the biological control of G. castaneae in chestnut groves. The exploitation of Trichoderma spp. as biopesticides is a novelty in the forestry sector and proves the benefits of these microbes in plant disease protection.

Production of Chestnut Coppice Biomass in a Framework of Low Mechanization and High Expectations to Combat Climate Change and Other Social Expectations

Climate change has become one of the most critical problems facing modern society. Sustainable forest management can be an important solution to counter the increasing concentration of carbon dioxide in the atmosphere. In particular, management of the chestnut forest could prove to be an effective strategy to absorb carbon dioxide as this species is characterized by sustained growth, so it has a high capacity to store carbon, and through the use of wood products, it is possible to sequester it for a considerable period. Chestnut (Castanea sativa Mill.) forests cover an area of about 800,000 ha in Italy, most of which is managed as coppice. It plays a central role in the Latium Region where its productive function is very important, as it provides timber of excellent quality. The purpose of this paper is to verify whether the current management of chestnut is efficient, as well as whether retractable wood products can contribute to the fight against climate change. The chestnut coppice located in the municipality of Tolfa (Lazio region, Italy) produces timber for 352 m3/ha and stores about 390,000 kg of CO2. Wood residues and losses during woodworking, together with emissions for the use of machineries, generate emissions of 368,000 kg of CO2. The chestnut semi-finished products, with long-term use prospects, retain a net volume of 22,000 kg of CO2. Although this is good for combating climate change, the amount of CO2 stored is very low, less than 6% of the CO2 stored by functional unit. Chestnut wood has a high versatility of use, so it could replace several products generated by fossil raw materials. Moreover, the implementation of precision forestry, the adoption of forest management more oriented to favor larger plants, the development of local economies and the reduction in the carbon footprint of the wood supply chain through the use of sustainable technologies would increase the capacity for climate change mitigation and increase the added value of its products.

Isolation of Hydrolyzable Tannins from Castanea sativa Using Centrifugal Partition Chromatography.

Castanea sativa wood is a rich source of hydrolyzable tannins, known for their diverse bioactivities. To investigate these bioactive properties further, it is crucial to isolate and characterize hydrophilic compounds effectively. To address this issue, we developed a centrifugal partition chromatography (CPC) method and applied it to an aqueous C. sativa wood extract. We determined the partition coefficients (KD) of the six major compounds using four butanol-/water-based biphasic solvent systems. Initially, we utilized the n-butanol/propanol/water (3:1:4, v/v/v) systems for the first fractionation step. Subsequently, we employed the water/methyl tert-butyl ether/butanol/acetone (8:5:3:4, v/v/v/v) system to fractionate moderately and highly hydrophilic fractions. We calculated the KD values for major compounds of the most hydrophilic fractions using the butanol/ethanol/water (4:1:5, v/v/v) and butanol/isopropanol/water (2:1:3, v/v/v) systems. In total, we isolated 23 compounds through a combination of CPC, size exclusion chromatography, and preparative HPLC. Among these compounds, six have never been previously described. We characterized them by 1D and 2D NMR experiments and high-resolution mass spectroscopy acquisitions.

Investigations of the spatial and climate characteristics of natural pure chestnut (Castanea sativa Mill.) forests: A case of Zonguldak Regional Directorate of Forestry

Chestnuts with high economic and socio-cultural value (Castanea sativa Mill.) must determine forests' spatial and climatic characteristics to improve them, increase fruit yield, and effectively combat diseases and pests. This study aimed to determine the spatial and climatic attributes of pure chestnut forests spreading within the borders of the Zonguldak Forestry Regional Directorate. A total of six criteria were used for spatial and climatic analyses: total precipitation (mm/year), annual mean temperature (C°), wind speed (m/s), altitude (m), slope (%), and aspect (°). Climate values were calculated as maximum, minimum, and average values, respectively; 1 619.25, 866.95, and 1 024.07 mm/year for total precipitation (mm/year), 13, 6 and 11.76 C° for annual mean temperature (C°), 6.62, 0.22 and 2.46 m/s for wind speed (m/s). The spatial characteristics were calculated as 1 221.68, 33.24, and 300.23 m for the height (m), 32.43%, 7.34%, and 18.63% for the slope (%) and finally, 338.63°, 18.18° and 184.18° for the angle (°), respectively, in terms of maximum, minimum and average values. Bartın Forest Management Directorate (FMD) ranks first with 57.18% of the natural chestnut forests spread the most in the study area, while Zonguldak FMD ranks second with 21.45% and Ulus FMD ranks third with 17.13%. The results obtained from the study based on the location and climate will contribute to the selection of the most suitable place for the new chestnut forest afforestation studies to be established and increase the percentage of success.

Low persistence of Phytophthora ramorum (Werres, De Cock, and Man in ‘t Veld) in western France after implementation of eradication measures

Key messagePresence of Phytophthora ramorum (Werres, De Cock, and Man in ‘t Veld) in western France was studied after the detection of this invasive pathogen in 2017 in Larix kaempferi (Lamb.) and eradication of the affected stands. P. ramorum was seldom detected in the area of the outbreak in the year following eradication. However, we confirm that P. ramorum can multiply to epidemic level on chestnuts (Castanea sativa Mill.) in the absence of larch (Larix spp.). This represents the major risk in France.ContextPhytophthora ramorum is an invasive oomycete that causes significant damage in the USA and Europe. Although the pathogen has been present in nurseries in France since 2002, the first outbreaks in forest stands were identified in 2017 in plantations of Larix kaempferi in two forests in western France (Saint-Cadou and Hanvec). In order to limit the development of the epidemic, neighboring larch stands were clear-cut.AimThis study investigated the presence of P. ramorum in the affected area after the eradication treatment.MethodsLarch stands located within a 18-km radius of the reported outbreaks were investigated. We also monitored the native woody hosts present in infected clear-cut larch stands and in the vicinity of seven ornamental nurseries that had been infected by P. ramorum on several occasions in the past.ResultsOverall, a very limited presence of P. ramorum was detected in 2018–2021. Two new stands of infected L. kaempferi were found close to the main initial outbreak, in Saint-Cadou and Saint-Rivoal. The pathogen was only detected on rhododendrons and chestnut trees (Castanea sativa Mill.) in the vicinity of the outbreaks. In the Saint-Cadou state Forest, an outbreak of the disease developed in 2019–2021 on chestnut trees even though all the mature larch trees had been felled. P. ramorum was also detected near two of the formerly infected ornamental nurseries, on Castanea sativa and on rhododendrons.ConclusionWhile larches and rhododendrons are uncommon in the forests of north-western France, chestnut trees are present in 21–25% of the forest and therefore represent the major risk for the survival of P. ramorum in the region.

EIF4A3 targeted therapeutic intervention in glioblastoma multiforme using phytochemicals from Indian medicinal plants – an integration of phytotherapy into precision onco-medicine

Glioblastoma Multiforme (GBM), an aggressive brain tumor (grade-IV astrocytoma), poses treatment challenges. Poor prognosis results from the rapid growth, highlighting the role of EIF4A3 in regulating non-coding RNAs. EIF4A3 promotes the expression of several non-coding RNAs, viz, Circ matrix metallopeptidase 9 (MMP9), a prominent oncogene, by interacting with the upstream region of the circMMP9 mRNA transcript and acts on cell proliferation, migration, and invasion of GBM. However, research shows that EIF4A3 knockdown inhibits glioblastoma progression and increases apoptosis. In this study, we explored the efficiency of the phytochemicals from plants like Withania somnifera and Castanea sativa with potential anti-glioblastoma effects as obtained from the Indian Medicinal Plants, Phytochemistry and Therapeutics (IMPPAT) database. Consequently, we have performed a virtual screening of the compounds against the protein EIF4A3. We further investigated the efficiency of the shortlisted compounds based on docking scores evaluated using GOLD, AutoDock4.2, LeDock, and binding free energy analyses using Molecular Mechanics Poisson–Boltzmann Surface Area (MMPBSA). Among the phytochemicals studied so far, several Withania-specific compounds from Withania somnifera and a single dietary compound, viz., Thiamine from Castanea sativa, have exhibited comparatively good blood-brain barrier permeability, significant binding affinity towards the EIF4A3, and good ADMET properties. Furthermore, we have verified the interaction stability of the lead molecules with EIF4A3 using MD simulations. Thus, the present study offers an opportunity to develop drug candidates targeting glioblastoma caused by EIF4A3 over-expression, integrating phytotherapy into precision oncology to create tailored and precise natural treatment strategies for cancer. Communicated by Ramaswamy H. Sarma

Classification of hybrid chestnut cultivars (Castanea sativa) registered in Türkiye with artificial neural networks, based on some physical properties of their nuts

Classification of hybrid chestnut cultivars (Castanea sativa) registered in Türkiye with artificial neural networks, based on some physical properties of their nuts

Damage to Sweet Chestnut Orchards in Croatia due to Invasive Alien Bark Beetle Xylosandrus germanus

Black stem borer, Xylosandrus germanus (Coleoptera: Scolytinae), was first recorded in Croatia in 2009. Until now, the insect has been reported only as a part of ambrosia beetles entomofauna in oak stands (Quercus robur and Quercus petraea). In 2021, severe attack of ambrosia beetles causing tree decline and mortality have been observed in three orchards of hybrid sweet chestnut (Castanea sativa x Castenea crenata). Orchards near Topusko, Marija Bistrica and Vugrovec were inspected for the symptoms. Twenty-seven adult ambrosia beetle specimens were collected from eight samples of damaged sweet chestnut trees, and were identified according to their morphology. All 27 ambrosia beetles were morphologically identified as X. germanus. This is the first record of black stem borer causing damage on cultivated sweet chestnut in Croatia. Considering similar reports from Italy and Slovenia, X. germanus could be regarded as a new, potentially damaging pest in chestnut plantations.

Chestnut blight (Cryphonectria parasitica (Murr.) Barr) disease incidence and its effect on the morphological and anatomical features of Castanea sativa trees

AbstractSweet chestnut (Castanea sativa) is an ecologically and economically valuable tree species worldwide. Various pathogens, both endemic or introduced, cause severe damage or death to chestnut trees. Chestnut blight, caused by the fungus Cryphonectria parasitica, is one of the most devastating plant diseases known which influences chestnut trees in all major cultivation regions. The work presented here aimed to investigate how the incidence of chestnut blight varied between trees growing at two altitudes (200–400 m vs. 400–600 m) and to determine the impact of chestnut blight on tree‐ring width, morphology and anatomy of C. sativa trees. The incidence of C. parasitica infections was greater at 200–400 m than at 400–600 m. Greater stem diameters were found at 200–400 m than at 400–600 m. There were no differences in stem diameters and heights, however, between healthy and C. parasitica infected chestnut trees. The anatomical characteristics of wood were also compared between healthy and C. parasitica‐infected chestnut trees. Fibre cell traits (length/width, lumen width, lumen area) were higher in healthy trees than in C. parasitica‐diseased trees. Vessel diameters were greater in infected compared with healthy trees. Tree ring widths and wood anatomical characteristics therefore were significantly influenced by C. parasitica infections.

Effects of the Abandonment of Traditional Cultural Practices on Epiphytic Bryolichenic Communities in Chestnut Orchards in North-West Spain

This study delves into the impact of contrasting management practices on epiphytic bryolichenic communities, shedding light on their divergent responses to management regimes on Castanea sativa Mill. orchards. Lichens and bryophytes were sampled in managed and abandoned plots, in 95 trees, in north and south sides and at two heights in Galicia (NW Spain). The studied groups exhibited opposing reactions to these management practices; bryophytes suffered adverse effects in managed stands, experiencing reduced cover and species richness compared to abandoned orchards, while lichens displayed heightened cover and species diversity. The size of trees, included as a covariate in our analyses, displayed no significant impact on the overall species richness of lichens or bryophytes, although it did influence the cover of specific functional traits. These differing outcomes may be linked to alterations in environmental conditions brought by management interventions. Furthermore, the study uncovered divergent responses within the taxonomic and functional composition of epiphytic communities. Different species and functional groups exhibited varying reactions to changing environmental conditions, making predictions a complex endeavour. In conclusion, this research emphasizes the need for management strategies that account for the diverse ecological requirements of different species and functional groups since no single management regime will suit all species or functional groups.

Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems.

Shoots from European chestnut and hybrids of European and Asian chestnuts can be efficiently proliferated in liquid medium by temporary immersion (TIS) using RITA® and Plantform™ bioreactors. The main challenges of applying TIS to these species were the lack of growth and hyperhydricity; problem solutions included the manipulation of the hormone concentration, the explant type, immersion frequency, and a support for maintaining shoots in a vertical position. After protocol optimization, explants cultured by TIS produced more rootable shoots than explants growing in semisolid medium, enabling increased number of rooted and acclimatable shoots. In this chapter, we will describe the protocols for proliferating chestnut by TIS in RITA® and Plantform™ bioreactors, together with tips for avoiding the main pitfalls of the technique. The strategies applied to chestnut can be useful for culturing other woody plants in bioreactors.

Assessing climate change impacts on the spatial distribution of Castanea sativa Mill. using ecological niche modeling

In recent decades, ecological niche modeling (ENM) has become integral for assessing climate change impacts on species distributions. In this study we conducted a comprehensive ENM using the Kuenm R package, employing MaxEnt as the modeling algorithm, to evaluate the impact of climate change on the habitat of Castanea sativa Mill., a non-wood forest species of high commercial interest in Turkiye, within the limits of the Trabzon Regional Directorate of Forestry (RDF). Predictors related to the species' ecology were carefully selected. The Future distributions of C. sativa for 2061–2080 under Shared Socio-economic Pathways (SSPs) 1-2.6, 2-4.5, and 5-8.5 were modeled using predictions from the Hadley Centre Global Earth Model HadGEM-GC31-LL. Extensive calibration modeling with Kuenm resulted in 434 models, and the most robust model, determined by statistical significance, predictive power, and complexity, revealed a drastic reduction in suitable areas for C. sativa (ranging from 86% to 99% across SSPs). The critical values of bio1 and bio5 were identified as primary factors. Predictions suggest potential migration of C. sativa to higher latitudes or elevations seeking more favorable climatic conditions. The substantial reduction in habitat suitability, even under SSP1-2.6, poses a significant threat, emphasizing the need for urgent measures to mitigate climate change impacts and ensure the species' survival and continuity.