Age Span Research Articles

Hematology reference intervals in 6–12-year-old children: the health-oriented pedagogical project (HOPP)

Reference intervals are essential for correct interpretation of laboratory test results, supporting clinicians in distinguishing between healthy and sick individuals. The present study aims to establish pediatric reference intervals for hematological parameters based on a large population of healthy schoolchildren. Blood samples were obtained from 1351 children 6–12 years of age participating in the Health-Oriented Pedagogical Project (HOPP). Reference intervals for hematological parameters were estimated by the nonparametric method following the CLSI C28-A3 guidelines. Reference intervals were estimated as 2.5th and 97.5th percentiles with corresponding 90% confidence intervals. While hematocrit and MCV required age and sex partitioning, hemoglobin and erythrocytes were partitioned for age. The remaining parameters, MCH, MCHC, platelets and white blood cell counts did not require partitioning. While red blood cell parameters exhibited an increasing trend with age, there was a slight decrease in leukocytes, lymphocytes, basophils and platelets with age. The remaining parameters were stable across our age span.

Recreational Football and Bone Health: A Systematic Review and Meta-analysis.

Recreational football is an intense, versatile form of exercise with multiple high- and odd-impact actions. Recreational football is therefore hypothesized to be suitable for bone modeling and bone health. The aim of the present systematic review and meta-analysis was to evaluate the effects of recreational football on bone mineral density (BMD), bone mineral content (BMC) and bone turnover markers (BTM). Systematic review and meta-analysis. MEDLINE, PubMed, SPORTDiscus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL) and Google Scholar were searched prior to September 2021. A manual database search was also performed using the following key terms, either singly or in combination: recreational football/soccer, street football/soccer, recreational small-sided games, effect, influence, impact, bone turnover markers, bone mineral density, bone turnover marker, bone health, osteogenesis, CTX, osteocalcin, P1NP. Randomised and matched controlled trials with participants allocated to a recreational football group or any other type of training intervention or passive control group were included. The primary outcome measures were total BMD, lower limb BMD, total BMC, lower limb BMC, osteocalcin, procollagen type 1N-terminal propeptide (P1NP) and collagen type 1 cross-linked C-telopeptide (CTX). A total of 17 papers met the inclusion criteria and were included. Comprehensive Meta-analysis V.2 software (Biostat, Englewood, New Jersey, USA) was used for the meta-analyses. Agreement between the two reviewers was assessed using RoB2 tool and k statistics for full-text screening and rating of relevance and risk of bias. The k agreement rate between reviewers was k = 0.92. The football interventions included were based on studies having a duration of 12-64weeks with one 5-year follow-up study and with a training frequency of 1-3 sessions/wk. Training sessions were 45-60min sessions of 3v3 - 7v7 small-sided games. The subjects covered an age span from 9 to 73years. Five studies examined recreational football effects in females, nine studies in males and three studies included both sexes. Recreational football training produced a statistically significant effect (mean difference = 0.02g/cm2, 95% confidence interval: 0.00-0.03, P = 0.02) on lower limb BMD and negligible effects for total BMD compared to no-exercise controls. The recreational football effects on total and lower limb BMC, when compared to both no-exercise controls and exercise controls, were negligible. A moderate to large significant increase in osteocalcin, P1NP and CTX was observed for recreational football compared to both no-exercise controls and exercise controls. In conclusion, recreational football training regimes lasting 12-64weeks have a large osteogenic impact on bone turnover markers in comparison with no-exercise controls as well as exercise controls, and beneficial effects on lower limb BMD compared to no-exercise controls. Short and medium duration recreational football interventions have negligible effects on whole-body BMD and BMC (total and lower limb), with magnitudes similar to those of other exercise modes.

Multi-center Retrospective Study of Factors Affecting Perioperative Transfusion of Packed Red Blood Cells for Pelvic Fracture Patients.

ObjectiveTo analyze the use of packed red blood cells (PRBCs) for patients with pelvic fracture and evaluate factors associated with PRBC transfusion for patients with pelvic fracture.MethodsThis retrospective cohort study collected 551 patients with pelvic fractures from six hospitals between September 1, 2012, and June 31, 2019. The age span of patients varied from 10 to 95 years old, and they were classified into two groups based on high‐energy pelvic fractures (HE‐PFs) or low‐energy pelvic fractures (LE‐PFs). The study's outcome was the use of PRBCs, fresh frozen plasma (FFP), and albumin. Demographic data, characteristics, laboratory tests, clinical treatment details, and clinical outcomes were compared between the two groups. Factors that were statistically associated with perioperative PRBCs in univariate analyses were included to conduct an optimal scale regression to determine the independent factors for perioperative PRBCs.ResultsA total of 551 patients were screened from six hospitals, and after inclusion and exclusion, 319 were finally included and finished the follow‐up from admission to discharge, while four patients died during hospitalization. Three hundred and nineteen patients were classified into two groups by their injury mechanisms. A total of 230/319 (72.1%) patients were classified into the HE‐PF group, and 89/319 (27.8%) patients were classified into the LE‐PF group. Patients in the HE‐PF group were transfused with 4.5 (3–8) units of PRBCs, 300 (0–600) ml of FFP, and 0 (0–30) g of albumin, while patients in the LE‐PF group were transfused with 3.5 (2–4.5) units of PRBCs, 0 (0–295) ml of FFP, and 0 (0–0) g of albumin (all P < 0.001). There were higher proportions of male patients and patients under 65 in the HE‐PF group (all P < 0.001). HE‐PF group patients were more severely injured and likely to take external fixation. The optimal scale regression revealed four significant factors associated with perioperative transfused PRBCs, which were patients on admission with hemorrhagic shock (importance = 0.283, P = 0.004), followed by fracture types identified by Tile classification (importance = 0.156, P < 0.001), hemoglobin levels below 70 g/L on admission (importance = 0.283, P = 0.004), followed by fracture types identified by Tile classification (importance = 0.156, P < 0.001), hemoglobin levels below 70 g/L on admission (importance = 0.148, P = 0.039), and methods of pelvic fixation (importance = 0.008, P = 0.026), ranked by the importance.ConclusionPatients with HE‐PFs had increased transfusions of PRBCs, FFP, and albumin, and hemorrhagic shock on admission, Tile classification, Hb levels, and stabilization methods were found to be associated with perioperative PRBCs.

Defining Referral Regions for Inpatient Trauma Care: The Utility of a Novel Geographic Definition.

Geographic variation is an inherent feature of the US health system. Despite efforts to account for geographic variation in trauma system strengthening, it remains unclear how trauma "regions" should be defined. The objective of this study is to evaluate the utility of a novel definition of Trauma Referral Regions (TRR) for assessing geographic variation in inpatient trauma across the age span of hospitalized trauma patients. Using 2016-2017 State Inpatient Databases, we assessed the extent of geographic variability in three common metrics of hospital use (localization index, market share index, net patient flow) among TRRs and, as a comparison, trauma regions alternatively defined based on Hospital Referral Regions, Hospital Service Areas, and counties. A total of 860,593 admissions from 102 TRRs, 127 Hospital Referral Regions, 884 Hospital Service Areas, and 583 counties were included. Consistent with expectations for distinct trauma regions, TRR presented with high average localization indices (mean [standard deviation]: 83.4 [11.7%]), low market share indices (mean [standard deviation]: 11.9 [7.0%]), and net patient flows close to 1.00. Similar results were found among stratified pediatric, adult, and older adult patients. Associations between TRRs and variations in important demographic features (e.g., travel time by road to the nearest Level I or II Trauma Center) suggest that while indicative of standalone trauma regions, TRRs are also able to simultaneously capture critical variations in regional trauma care. TRRs offer a standalone set of geographic regions with minimal variation in common metrics of hospital use, minimal geographic clustering, and preserved associations with important demographic factors. They provide a needed, valid means of assessing geographic variation among trauma systems.

Proposal for Growth and Development Evaluation Chart of Physical Abilities in Preschool Children-Analysis based on wavelet interpolation model-

Growth and development evaluations of physical abilities (physique, motor ability) in preschool children are generally done with uniform evaluation charts of evaluation ranges based on cross-sectional data. In recent years, objective evaluation methods with least squares approximation polynomials have also been established. However, variability cannot be ruled out when using cross-sectional data on the growth and development of physical abilities in preschool children, and so physical development evaluations based on longitudinal changes with age, taking date of birth into account, have not been established. Of course, there are times when methods for constructing evaluations cannot be established. In this study, we applied the wavelet interpolation model to physical ability measurements corresponding to year and month of age, in order to evaluate growth and development of physical abilities associated with longitudinal aging over three years in boys 3 to 5 years old in Japan. First, standard age-related changes were investigated. Physical growth fundamentally tends to occur gradually, but body weight increases with slight fluctuations. In standard development of motor ability, particularly in the 20-m dash, fluctuating trends were seen near 3 years old, but there was a converging tendency in fluctuations associated with aging. In the tennis ball throw, the biological variation was pronounced, and there were large individual differences. Based on these trends, the effectiveness of applying the wavelet interpolation model became clear in this study. Hence, we applied the wavelet interpolation model to the mean values and standard deviations for the growth and development of physical abilities associated with aging over a span of three years during early childhood, and constructed an age span evaluation chart. With this evaluation chart, an evaluation that can correspond flexibly to growth and development in physical ability could be said to have been constructed.

How language skills and working memory capacities explain mathematical learning from preschool to primary school age: Insights from a longitudinal study.

Between the age span of 3 to 6 years the foundation for children’s mathematical learning (i.e., numerical abilities and cognition) are laid. However, the developing relations between mathematical skills, language, and working memory starting at preschool age and evolving into primary school age are not well understood. Adopting an empirically validated analysis model, the present study examines in detail longitudinal interdependencies between mathematical skills, a wide range of language skills, and working memory components underlying the mathematical learning process of 41 German preschool children (41.5% female) spanning ages 4 to 6. Phonological processing skills and expressive grammar skills emerged as the most significant language skills for the process of children’s mathematical learning across the investigated age span. Within the latter, children’s phonological processing skills and expressive grammar skills were supported by children’s word expression abilities. The phonological loop emerged as the most important working memory component for children’s early mathematical learning between ages 4 to 6. Furthermore, a wide array of language skills were associated with complex information and storage processes within this mathematical learning process. In conclusion, the present findings provide a more detailed and deeper insight into the learning process of children’s number concept, emphasizing the influence of phonological and particularly grammatical skills.

New U-Pb zircon ages of plagiogranites from the Coastal Complex ophiolite and Twillingate batholith, Newfoundland: Evidence for the oldest and overlapping silicic magmatism in the nascent Cambrian peri-Laurentia forearc and arc terranes

The Coastal Complex (CC) and Bay of Islands Complex (BOIC) represent two supra-subduction zone (SSZ) forearc ophiolitic assemblages of different ages within the Taconic Humber Arm Allochthon of the Western Newfoundland Appalachians. To further constrain age relationships between the CC and BOIC and earliest initiation of Iapetus subduction, we acquired LA-ICP-MS U-Pb zircon ages for eleven plagiogranites sampled along strike of the CC belt. These ages combined with three prior legacy ages establish an age range of 514.3–502.7 Ma for the six CC plagiogranite plutons. The oldest precise ca. 514.3 Ma CC pluton age represents the oldest Taconic arc-related plutonic activity yet documented in Western Newfoundland and provides a minimum age for the intruded CC ophiolite basement and initiation of westerly vergent subduction within the peri-Laurentian Iapetus Ocean. This confirms the Early Cambrian CC mafic–ultramafic basement is significantly older than the juxtaposed SSZ BOIC, which formed by later rifting of the CC forearc along younger forearc spreading center in the Late Cambrian by ca. 488.3 Ma. After subduction initiation a prolonged early period CC forearc silicic magmatism lasted at least ∼ 11.6 Ma until ca. 502.7 Ma, a range consistent with age spans of magmatism in modern juvenile forearcs. We also reinvestigated the Central Newfoundland Twillingate Granite batholith thought to be one of the earliest major felsic batholiths formed within the adjacent juvenile arc massif of the Notre Dame Subzone, part of the same peri-Laurentian arc. We determined a precise 3-sample composite age of 504.3 ± 1.8 Ma that signals the formation of earliest Notre Dame arc edifice developed by Middle Cambrian. It overlaps the end-stage CC forearc plagiogranite magmatism and places a minimum mid-Cambrian age constraint on intruded ophiolitic arc tholeiitic and boninitic pillow lavas of the Sleepy Cove and correlated Lushs Bight volcanics, suggesting basement continuity with the CC.

Referral patterns, clinical features and management of uncorrected hypospadias in a series of adult men

Hypospadias surgery undertaken in early life often continues to impose challenges as patients age. Little is known about the natural history of uncorrected hypospadias persisting into adulthood. To describe presenting symptoms and management strategies in men with uncorrected hypospadias referred to our national tertiary transitional clinic for congenital urological conditions. Patients with uncorrected hypospadias older than 16 years at the time of referral were identified by searching the electronic patient record system for ICD-10 hypospadias codes. Data were extracted over a 10-year period according to a predefined protocol. Among 201 referrals, 65 men with hypospadias (glanular n=12, coronal n=26, subcoronal n=9, corporal n=4, penoscrotal n=2 and MIP n=12) had never previously had reconstructive surgery undertaken. Obstructive symptoms predominated (n=30) and the risk of symptoms increased with advancing age (Figure). Presenting complaints varied across the age span; cosmetic issues (n=11) and coital pain (n=5) were primarily seen in youth as opposed to urinary obstructive symptoms that were increasingly more frequent with age (p=0.002) (Figure). Management included reconstructive surgery (n=24), minor procedures (preputioplasty, circumcision, meatoplasty, dilatation/urethrotomy, total n=28) as well as counselling (n=12). The management strategies were independent of age and hypospadias type. The current cohort delineates the dynamic nature of hypospadias in itself. We speculate that the distinction in the primary complaint leading to referral between the extremes of age may relate to the vanity and insecurity of youth while older patients first come forward when other symptoms arise. Dissatisfaction with genital appearance is uncommon in previous smaller studies on men with uncorrected hypospadias unlike in our study, where 11 patients were assessed mainly for cosmetic concerns. Obstruction is the main symptom encountered in adult hypospadias patients operated in early life, and a similar picture was observed in our cohort of unoperated cases. Urethral dilatation and internal urethrotomy are temporizing procedures but were successful in immediate alleviation of obstructive symptoms in patients not willing to consign themselves to formal surgery. The study is limited by its retrospective design, and our symptomatic cohort may also represent the extreme end of the hypospadias spectrum. Medical issues vary across the age span in men with unrepaired hypospadias. Minor surgical procedures as well as counselling play an equally important role as reconstructive hypospadias surgery in the management of unrepaired hypospadias in adulthood.

Large zircon age spans record multi-stage history of batholith assembly: Insights from the Late Triassic Dongcuo batholith in the eastern Tibetan Plateau

Large and homogeneous plutons can assemble incrementally over millions of years, yielding large age spans. Determination of the age span is crucial to constrain the timing and nature of the batholith assembly. This paper reports comprehensive whole-rock geochemical data, LA-ICP-MS zircon U-Pb age, and zircon Hf isotopic data to constrain the timing and assembly process of the Late Triassic Dongcuo batholith, the largest batholith in the Yidun Terrane, eastern Tibetan Plateau. Samples from the batholith consist of metaluminous to weakly peraluminous granitoids (SiO2 = 69.91–79.85 wt%). These granitoids show large variation of zircon Hf isotopic compositions with εHf(t) of -17.15 to +0.13. The multiple zircon populations in single hand specimens revealed by kernel density estimation spread over 20 Myr (ca. 225–204 Ma), which is also confirmed by core-rim ages of single zircon grains, suggesting prolonged magmatism of the Dongcuo batholith. Our data indicate that the assembly of the batholith resulted from emplacement of discrete magma batches which derived from spatially varied sources and underwent variable differentiation. The assembly mainly involved three stages, including an early episode of magmatism (ca. 228 Ma), a high-flux magmatism between ca. 220 Ma and ca. 216 Ma, and a late-stage thermal event (ca. 211–204 Ma) possibly related to high temperature and localized remelting of the batholith. This study highlights the complexity of zircon populations in composite granitic batholith, showing that the search for single crystallization ages for a sample is fraught with difficulties and may mask rather than elucidate the crystallization history of the magmatic body.

1010-P: Diabetes Distress in Younger vs. Older Emerging Adults: Confirmation of Content Validity of the Problem Areas in Diabetes—Emerging Adult Survey (PAID-EA)

The developmental stage of emerging adulthood covers a wide age span (ages 18-30 years) with potential for differing emotional burdens for those living with type 1 diabetes (T1D) . To further confirm the content validity of the PAID-EA and to investigate differences between younger and older emerging adults, item responses on the newly developed, previously validated PAID-EA survey were examined by age group. Emerging adults (N=287) with T1D completed online measures of diabetes distress, depressive symptomology, and the PAID-EA. The PAID-EA has 25 items and uses a 5 point Likert-type response scale with options ranging from agree to disagree. The sample was split into 2 age groups: younger emerging adults (ages 18-24, N=143) and older emerging adults (ages 25-30, N=122) . Self-reported HbA1c was significantly worse in younger vs. older emerging adults (7.4% vs. 6.8%, p&amp;lt;.001) . Other demographic variables and total scores on measures were similar between age groups. Only 3 items on the PAID-EA differed by age group with younger emerging adults endorsing more diabetes distress: (1) Item 6: “I worry about being able to socialize because of how alcohol affects my blood sugar” (p=.03) , (2) Item 7: “I worry that a new romantic partner will see my diabetes devices or supplies” (p=.04) , (3) Item 25: I feel like I am trying my hardest to take care of diabetes, but it never works” (p=.02) . Experimentation with alcohol and intimacy with new partners may be experienced differently for younger compared with older emerging adults. Intense feelings of burnout and failure may also differ; this finding requires further exploration. Though emerging adulthood spans more than a decade, perceptions of the emotional burden of living with T1D appear consistent for the younger and older age groups, confirming the content validity of the PAID-EA throughout emerging adulthood. Disclosure K.Wentzell: None. R.J.Vitale: None. L.M.Laffel: Advisory Panel; Medtronic, Roche Diabetes Care, Consultant; Boehringer Ingelheim International GmbH, Dexcom, Inc., Dompé, Insulet Corporation, Janssen Pharmaceuticals, Inc., Lilly Diabetes, Novo Nordisk, Provention Bio, Inc. Funding Joslin DRC: P30DK036836 NIH: T32DK007260, T32DK007529

The codevelopment of reading and attention from middle childhood to early adolescence: A multivariate latent growth curve study.

Attention skills are strong cross-sectional predictors of reading comprehension from childhood through to adolescence. However, less is known about the developmental relations between these two domains across this period. This study examined the codevelopment of reading and attention in a community sample of 614 Australian school students (50% female). Reading and attention were assessed at ages 8, 10, 12, and 14. Results of univariate latent growth models demonstrated, on average, curvilinear trajectories for reading in which rapid growth across younger age spans decelerates as children reach adolescence. By contrast, attention skills remained relatively stable on average. Significant negative correlations were observed between the intercept and slope factors in separate reading (r = -.62) and attention models (r = -.39) suggesting compensatory growth patterns in which poorer performing students in both domains at age 8 have steeper trajectories than their higher performing peers. A comparison of a multivariate latent growth model and an autoregressive latent trajectory model with structured residuals (ALT-SR) examined the interrelatedness of development in reading and attention. Both between-individual and within-individual cross-domain parameters showed reading and attention to be positively related at Grade 3, indicating an association between better attention and higher reading achievement at age 8. However, there was little evidence for interrelated growth across domains in this sample. The results contribute to theories which explain whether and how multiple cognitive domains codevelop over a substantial period of childhood and early adolescence. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

0191 Characteristics of sleep spindles across development in males with Duchenne/Becker Muscular Dystrophy Disorder

Abstract Introduction Sleep supports cognition, in particular, the consolidation of memories. Sleeping brain rhythms, such as slow oscillations (1hz) and spindles (9-15 Hz), play a key role in facilitating this consolidation. Our prior research reported age-associated declines in slow oscillations in Duchenne and Becker Muscular Dystrophy (DMD/BMD) (Simon et al., 2020). Here, we characterize age-associated changes in sleep spindle characteristics across development in this group. Methods Following our 2020 analysis, we retrospectively analyzed the clinical sleep studies of 28 DMD/BMD males (Age span: 4 to 20 years). We applied our spindle detection algorithm to six electrodes (F3, F4, C3, C4, 01, O2). We assessed spindle density, frequency, and amplitude based on age (child, early adolescent, and late adolescent). Results We conducted rmANOVAs to evaluate each spindle characteristics using within-factors (Stage and Electrode) and between-factor (Age). We found significantly more spindles with longer durations in N2 than N3; greater spindle density at frontal compared to occipital regions; and higher amplitudes at central compared to frontal sites. We found no age-associated changes in these spindle metrics. We did find an age-associated change in the frequency of spindles, with significantly greater average spindle amplitude increasing significantly with age. Conclusion In line with prior research, we found more spindles in N2 than N3 and greater spindle density at frontal compared to posterior electrodes. In contrast to our previous research demonstrating age-associated declines in slow oscillations, our current analyses show minimal age-associated changes in spindle characteristics from 4 to 20 years. Further analysis is required to assess for age-associated changes in spindle-slow oscillation coupling occur across development. Our findings have implications for functional changes in sleep-dependent cognition mechanisms across development in BMD/DMD. Support (If Any)

0218 N2 and wakefulness drive subjective sleep satisfaction in adults

Abstract Introduction The measurable aspects of brain function derivable from polysomnography (PSG) that are correlated with sleep satisfaction are poorly understood. Previously, a weak association of PSG measures with subjectively rated sleep depth and restfulness was shown. Using recent developments in automated sleep scoring, which remove the within- and between-rater error associated with human scoring, we revisit whether whole night PSG measurements are associated with sleep satisfaction. Additionally, we investigate if PSG data collected closer to wake time explains the subjective sleep experience better than whole night PSG. Methods Random Forest machine learning was used to investigate the relationship between PSG data from the Sleep Heart Health Study (N=3,165, middle-aged and older adults) and self-reported sleep satisfaction (restfulness, depth). PSG were rescored using a novel automated algorithm that generates both a sleep stage for each 15-s epoch as well as a stage matching probability. Data were also parsed into 20 minute-fragments based on time relative to wake. Results Models explained 30% of subjective sleep depth and 27% of subjective sleep restfulness, with a similar top four predictors: minutes of N2 and wake after sleep onset (WASO), sleep efficiency, and age, capturing 28% (restfulness) and 26% (depth) of the relative model variance. With increasing subjective sleep quality, there was a progressive increase in N2 and decrease in WASO of similar magnitude, without systematic changes in N1, N3 or REM. In comparing those with the best and worst subjective experience of sleep, there is a range of approximately 30 minutes more N2, 30 minutes less WASO, an improvement of sleep efficiency of 7-8%, and an age span of 3-5 years. Random Forest models derived from PSG fragments closer to the offset of sleep did not provide better explanatory power compared to the whole-night data set. Conclusion Approximately one-third of the variance in two measures of self-reported sleep experience can be explained by whole-night PSG variables, notably an increase in N2 and decrease in wake that led to improved sleep efficiency. Interventions that specifically target these may be suitable for improving the self-reported sleep experience. Support (If Any)

Detrital zircon ages from Archaean conglomerates in the Singhbhum Craton, eastern India: implications on economic Au-U potential

New U–Pb age and Hf isotope data obtained on detrital zircon grains from Au- and U-bearing Archaean quartz-pebble conglomerates in the Singhbhum Craton, eastern India, specifically the Upper Iron Ore Group in the Badampahar Greenstone Belt and the Phuljhari Formation below the Dhanjori Group provide insights into the zircon provenance and maximum age of sediment deposition. The most concordant, least disturbed 207Pb/206Pb ages cover the entire range of known magmatic and higher grade metamorphic events in the craton from 3.48 to 3.06 Ga and show a broad maximum between 3.38 and 3.18 Ga. This overlap is also mimicked by Lu–Hf isotope analyses, which returned a wide range in εHf(t) values from + 6 to − 5, in agreement with the range known from zircon grains in igneous and metamorphic rocks in the Singhbhum Craton. A smaller but distinct age peak centred at 3.06 Ga corresponds to the age of the last major magmatic intrusive event, the emplacement of the Mayurbhanj Granite and associated gabbro, picrite and anorthosite. Thus, these intrusive rocks must form a basement rather than being intrusive into the studied conglomerates as previously interpreted. The corresponding detrital zircon grains all have a subchondritic Hf isotopic composition. The youngest reliable zircon ages of 3.03 Ga in the case of the basal Upper Iron Ore Group in the east of the craton and 3.00 Ga for the Phuljhari Formation set an upper limit on the age of conglomerate sedimentation. Previously published detrital zircon age data from similarly Au-bearing conglomerates in the Mahagiri Quartzite in the Upper Iron Ore Group in the south of the craton gave a somewhat younger maximum age of sedimentation of 2.91 Ga. There, the lower limit on sedimentation is given by an intrusive relationship with a c. 2.8 Ga granite. The time window thus defined for conglomerate deposition on the Singhbhum Craton is almost identical to the age span established for the, in places, Au- and U-rich conglomerates in the Kaapvaal Craton of South Africa: the 2.98–2.78 Ga Dominion Group and Witwatersrand Supergroup in South Africa. Since the recognition of first major concentration of gold on Earth’s surface by microbial activity having taken place at around 2.9 Ga, independent of the nature of the hinterland, the above similarity in age substantially increases the potential for discovering Witwatersrand-type gold and/or uranium deposits on the Singhbhum Craton. Further age constraints are needed there, however, to distinguish between supposedly less fertile (with respect to Au) > 2.9 Ga and more fertile < 2.9 Ga successions.

Critical Consciousness and Wellbeing in Adolescents and Young Adults: A Systematic Review.

Youth experiencing systemic oppression(s) face heightened challenges to wellbeing. Critical consciousness, comprised of reflection, motivation, and action against oppression, may protect wellbeing. Wellbeing here refers to mental, socioemotional, and physical health. The aim of this systematic review was to synthesize research on the relationship between critical consciousness and wellbeing among adolescents and young adults (ages 12–29). Five databases (PsycInfo, PsychArticles, ERIC, Sociological s, and PubMed) were searched systematically using keyword searches and inclusion/exclusion criteria; 29 eligible studies were included. Results demonstrated that the critical consciousness and wellbeing relationship varied by critical consciousness dimension and age. The studies of adolescents most often focused on racial/ethnic marginalization and found critical motivation most strongly associated with better wellbeing. The studies of young adults focused on young adult college students and identified mixed results specifically between activism and mental health. Study methods across age spans were primarily quantitative and cross-sectional. Research on critical consciousness and wellbeing can benefit from studies that consider multiple critical consciousness dimensions, use longitudinal approaches, and include youth experiencing multiple and intersecting systems of privilege and marginalization.

Association of Depression and Anxiety With the Accumulation of Chronic Conditions

Longitudinal associations between comorbid depression and anxiety with the accumulation of chronic illnesses are unclear, and questions remain about the contributions associated with each condition in the increasing prevalence of multimorbidity. To compare the risk and rate of accumulating chronic conditions in people with depression, anxiety, and comorbid depression and anxiety vs individuals with neither depression nor anxiety. This cohort study used the Rochester Epidemiology Project medical records-linkage system to identify residents of Olmsted County, Minnesota, from January 1, 2005, to December 31, 2014, with follow-up ending December 31, 2017. The sample was divided into cohorts anchored at birthday ages of 20, 40, and 60 years. Individuals were classified at anchoring birthday age as having depression alone, anxiety alone, comorbid depression and anxiety, or neither depression nor anxiety (reference group), using electronically extracted diagnosis codes from the International Classification of Diseases, Ninth Revision (ICD-9) in the 5 years before each anchoring birthday. Data were analyzed from August 2020 through November 2021. Depression alone, anxiety alone, comorbid depression and anxiety, or neither depression nor anxiety (reference group). The main outcome was sex-specific risk, calculated as hazard ratios (HRs) and rates of accumulation, calculated as mean annual incidence rates per 100 person-years, of 15 common chronic conditions within each birthday age cohort through the end of study. Among the 40 360 individuals included across all 3 age cohorts, 21 516 (53.3%) were women. After balancing cohorts on race, Hispanic ethnicity, education level, body mass index, smoking status, and calendar year at index birthday, the risk of accumulating chronic conditions was significantly increased among women with depression alone (cohort aged 20 years: HR, 1.20 [95% CI, 1.02-1.42]; cohort aged 40 years: HR, 1.20 [95% CI, 1.10-1.31]; cohort aged 60 years: HR, 1.09 [95% CI, 1.02-1.16]) and women with comorbid depression and anxiety (cohort aged 20 years: HR, 1.60 [95% CI, 1.28-1.99]; cohort aged 40 years: HR, 1.41 [95% CI, 1.21-1.65]; cohort aged 60 years: HR, 1.29 [95% CI, 1.15-1.44]) compared with referent women in the same birthday cohorts and in men with comorbid depression and anxiety compared with referent men in the cohort aged 20 years (HR, 1.77 [95% CI, 1.08-2.91]). For women, the rates of accumulation of conditions were significantly higher across birthday cohorts in the comorbid depression and anxiety group compared with the depression alone group (eg, cohort aged 20 years: difference, 1.2 [95% CI, 0.2-2.1] per 100 person-years) and reference group (eg, cohort aged 20 years: difference, 1.7 [95% CI, 0.9-2.6] per 100 person-years). For men, compared with the reference group, the rates of accumulation of conditions were significantly higher in men with comorbid depression and anxiety in the cohort aged 20 years (difference, 1.4 [95% CI, 0.1-2.6] per 100 person-years) and in men with depression in the cohort aged 40 years (difference, 2.0 [95% CI, 0.8-3.2] per 100 person-years). In this cohort study, the risk of accumulating chronic conditions was increased with depression and comorbid depression and anxiety in women across the age span and in younger men with comorbid depression and anxiety. Compared with women without depression or anxiety, there was a more rapid rate of accumulation of chronic conditions in women with depression and anxiety individually and an even higher rate when depression and anxiety cooccurred.

Hypoglossal Motoneuron (XII MN) Survival Over the Lifespan in Pompe Disease

Glycogen Storage Disease II, more commonly known as Pompe disease, is a lysosomal storage disease characterized by a partial or complete loss of acid α‐glucosidase (GAA) activity. Lingual impairment and sleep disordered breathing are common symptoms of Pompe disease with a potential role of XII MN dysfunction in both cases. Through innervation of the tongue muscles, XII MNs contribute to swallowing, speech, and maintenance of upper airway patency. Studies of Pompe (Gaa‐/‐) mice have found molecular and histological markers of neurodegeneration, and a recent case study of a patient with late‐onset Pompe disease revealed atrophied and possible degenerating XII MNs. However, whether the absence of GAA activity in Pompe disease directly leads to death of XII MNs is not definitively established. Because the preponderance of evidence indicates progressive neurodegeneration in Pompe disease, here we tested the hypothesis that when XII MNs do not express GAA activity, there will be a progressive loss of these cells over the lifespan. A novel transgenic mouse model (ChAT‐cre+/+●Gaa+/‐●eYFP+/+●Flox‐Gaa+/+) was generated in which GAA activity is absent in ChAT positive neurons, which also carry a yellow fluorescent protein (YFP) marker. Thus MNs in this mouse model do not express GAA, but skeletal myofibers have normal GAA expression. Brainstem tissue was harvested in young (1‐3 months of age) and advanced age mice (21‐24 months). Tissues were cryosectioned at 20 μm increments across the entire XII motor nucleus. Sections were slide mounted and visualized under fluorescence microscopy to detect YFP. The XII motor nucleus was identified based on anatomical landmarks and MNs were identified based on location, size, morphology, and YFP fluorescence. Preliminary MN counts across the rostral‐caudal extent of the XII nucleus (n=3 per group) indicate an approximately 7% decline in detectable YFP‐positive XII MNs over the age span (young: 658±19; old 617±28). Further, the histological appearance of XII MNs showed striking differences between the two age groups. The MNs from older mice showed the prototypical hallmarks of neuronal lysosomal storage disease including a markedly enlarged soma and extensive vacuolization. These preliminary findings are consistent with the hypothesis that absence of GAA in XII MNs leads to a histopathological appearance and neurodegeneration. Ongoing studies are evaluating XII MNs in age matched wild type mice.

Non-Right Handedness is Associated with More Time Awake After Sleep Onset and Higher Daytime Sleepiness Than Right Handedness: Objective (Actigraphic) and Subjective Data from a Large Community Sample.

PurposeHandedness has been linked to various physiological and pathological phenomena including memory function and psychiatric disorders. Also for sleep, several studies have reported associations. However, large-scale studies including a broad age span of participants and studies analyzing women and men separately are lacking.MethodsTherefore, objective sleep data were determined using at-home actigraphy from 1764 healthy participants (18 to 80 years, 908 women), averaging five consecutive nights. In addition, subjective sleep-related data were captured by self-report diaries, the Pittsburgh Sleep Quality Index (PSQI), the Epworth Sleepiness Scale (ESS) and the Morningness-Eveningness-Questionnaire (MEQ). Handedness was determined with the Edinburgh Handedness Inventory (EHI) providing information on the direction (left vs right) and the degree of handedness (strong vs weak). To address the potential endocrine effects, premenopausal women (≤45 years) and postmenopausal women (≥55 years) were analyzed separately. This was also done for men.ResultsThe degree and direction of handedness were correlated with “wake after sleep onset” (WASO) in the total sample and all women (the more right-handed/lateralized the shorter WASO). In postmenopausal women, additionally, time in bed (TIB) and total sleep time (TST) were correlated. There were no other significant associations between an objective sleep variable and handedness. In both premenopausal women and >55-year-old men subjective quality of sleep (PSQI) was correlated with direction and degree of handedness (the more right-handed/lateralized the better). In the total sample and postmenopausal women, the degree and direction of handedness were negatively correlated with daytime sleepiness. The chronotype was not associated with handedness in any group.ConclusionWhile associations were not consistent in all groups, overall, right-handedness tended to be associated with better sleep and less daytime sleepiness. Handedness and sleep seemed to be differentially associated in women and men, being in line with endocrine interactions.

Self-reported sleep and circadian characteristics predict alcohol and cannabis use: A longitudinal analysis of the National Consortium on Alcohol and Neurodevelopment in Adolescence Study.

Growing evidence indicates that sleep characteristics predict future substance use and related problems. However, most prior studies assessed a limited range of sleep characteristics, studied a narrow age span, and included few follow-up assessments. Here, we used six annual assessments from the National Consortium on Alcohol and Neurodevelopment in Adolescence (NCANDA) study, which spans adolescence and young adulthood with an accelerated longitudinal design, to examine whether multiple sleep characteristics in any year predict alcohol and cannabis use the following year. The sample included 831 NCANDA participants (423 females; baseline age 12-21years). Sleep variables included circadian preference, sleep quality, daytime sleepiness, the timing of midsleep (weekday/weekend), and sleep duration (weekday/weekend). Using generalized linear mixed models (logistic for cannabis; ordinal for binge severity), we tested whether each repeatedly measured sleep characteristic (years 0-4) predicted substance use (alcohol binge severity or cannabis use) the following year (years 1-5), covarying for age, sex, race, visit, parental education, and previous year's substance use. Greater eveningness, more daytime sleepiness, later weekend sleep timing, and shorter sleep duration (weekday/weekend) all predicted more severe alcohol binge drinking the following year. Only greater eveningness predicted a greater likelihood of any cannabis use the following year. Post-hoc stratified exploratory analyses indicated that some associations (e.g., greater eveningness and shorter weekend sleep duration) predicted binge severity only in female participants, and that middle/high school versus post-high school adolescents were more vulnerable to sleep-related risk for cannabis use. Our findings support the relevance of multiple sleep/circadian characteristics in the risk for future alcohol binge severity and cannabis use. Preliminary findings suggest that these risk factors vary based on developmental stage and sex. Results underscore a need for greater attention to sleep/circadian characteristics as potential risk factors for substance use in youth and may inform new avenues to prevention and intervention.

Age and sex associations of SARS-CoV-2 antibody responses post BNT162b2 vaccination in healthcare workers: A mixed effects model across two vaccination periods.

This study aimed to examine the associations with epidemiological, behavioral and clinical parameters of IgG antibody responses against the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) after immunization with two doses of the BNT162b2 vaccine in a cohort of healthcare workers (HCWs, n = 439) in Greece. We used a mixed effects model to investigate the potential associations of antibody levels one and three months after vaccination and examined by bootstrapping t-tests the putative effects of gender and age for each period. We also employed exact tests of independence in R × C contingency tables to explore associations between behavioral and gender variables with vaccinations side effects. We found significant differences between males and females as well as between subjects in the youngest (21–30 years) and the older age groups in both study periods. We also detected a decrease in titers with age and time. Males had steeper elimination rates across the age span in both periods, in contrast to females who exhibited a softer elimination titer rate with age in the first period and almost constant titers in the second. Concerning side effects, we found a significant association between pain at the injection site and female sex. Hence, our real-world data analyses revealed potentially important clues into the associations of antibody responses to SARS-CoV-2 spike. We discuss the importance of these findings in view of current mass vaccination perspectives and provide useful clues for the design and optimal timing of booster doses for COVID-19.