Treatment Of Soybean Seeds Research Articles

Influence of fungicides on activities of enzymes of phenolic metabolism in the early stages of formation and functioning of soybean symbiotic apparatus

We studied the effect of complex seed treatment with fungicides and rhizobium culture on the activity of phenolic metabolism enzymes – polyphenol oxidase and guaiacol peroxidase in the early stages of the formation and functioning of various symbiotic systems Glycine max – Bradyrhizobium japonicum. In the research we used microbiological, physiological, biochemical methods, gas chromatography and spectrophotometry. The objects of the study were selected symbiotic systems formed with the participation of soybean (Glycine max (L.) Merr.), Diamond variety, strains Bradyrhizobium japonicum 634b (active, virulent) and 604k (inactive, highly virulent) and fungicides Maxim XL 035 PS (fludioxonil, 25 g/L, metalaxyl, 10 g/L), and Standak Top (fipronil, 250 g/L, thiophanate methyl, 225 g/L, piraclostrobin, 25 g/L). Before sowing, the seeds of soybean were treated with solutions of fungicides, calculated on the basis of one rate of expenditure of the active substance of each preparation indicated by the producer per ton of seed. One part of the seeds treated with fungicides was inoculated with rhizobium culture for 1 h (the titre of bacteria was 108 cells in 1 ml). The other part of the fungicide-treated seeds was not inoculated by rhizobium culture. As a result of the research, it was revealed that an effective symbiotic system formed with the participation of soybean plants and the active strain rhizobia 634b is characterized by a high level of polyphenol oxidase activity and low guaiacol peroxidase in roots and root nodules in the stages of second and third true leaves. Such changes in the activity of enzymes occurred along with the formation of nodules which actively fixed the molecular nitrogen of the atmosphere. An ineffective symbiotic system (strain 604k) is characterized by an elevated level of polyphenol oxidase activity in the roots and guaiacol peroxidase in the root nodules, which is accompanied by activation of the process of nodulation. Treatment of soybean seeds with fungicides in an effective symbiotic system leads to a change in the activity of the enzymes of the phenolic metabolism, which induced adaptive changes in plant metabolism and growth of nitrogenase activity of the root nodules. The recorded changes in the activity of both enzymes for the action of fungicides in the ineffective symbiotic system can be considered as a kind of response of the plant to the treatment and were observed along with the reduction of the processes of nodulation into the stage of the third true leaf.

Effect of Plasma Treatment of Soybean Seeds on Their Quality and Development of Seedlings

The effect of treatment with a low-temperature argon plasma of atmospheric pressure and dielectric barrier discharge plasma on MK100 variety soybean seed was studied under field and laboratory conditions of the All-Russia Scientific Research Institute of Soybean. Positive results on the biochemical composition and sowing qualities of soybean seeds have been obtained. In soybean seeds treated with plasma, protein enzymatic systems were activated and content of protein decreased by 1.3–1.5%. Under the influence of argon plasma, the number of abnormally germinated seedlings decreased by 1–5%, and the length of seedlings increased by 2.3–2.5 cm compared to the control. The positive effect of low-temperature argon plasma on seed germination promoted even sprouts under field conditions and increased the plant resistance to external stressors. The use of short-term treatment by plasma flow with gentle parameters provided an increase in germination and germinative power of soybean seeds and contributed to the increase in preservation of plants under field conditions, which guarantees the increase in harvest of seeds.

Effect of plasma treatment of soybean seeds on their quality and development of seedlings

Effect of plasma treatment of soybean seeds on their quality and development of seedlings

Addition of biostimulant to the industrial treatment of soybean seeds: physiological quality and yield after storage

Abstract: This study aimed to compare the results of germination, vigor, and yield of high- and low-vigor soybean seeds submitted to on-farm application of biostimulant with its application via industrial treatment 60 days before sowing. The experimental design was a factorial arrangement of 6 × 2 × 2 (technology × vigor level × strategy of biostimulant application) for the laboratory experiment and of 6 × 2 (technology × strategy of biostimulant application) for the field experiment. Germination, accelerated aging, emergence in sand, seedling dry biomass, and electrical conductivity were assessed in the laboratory, while stand, one thousand-grain weight, and yield were assessed in the field. Biostimulant application in soybean seeds not coated with pesticides did not affect the physiological quality regardless of the application moment (at the day of sowing or at 60 days before sowing). However, when applied in seeds coated with fungicides and insecticides, better results of germination, vigor, and yield were obtained with the on-farm use of biostimulant.

Treatment of soybean seeds of different levels of physiological quality with amino acids

Abstract: The aim of this study was to assess the effect of seed treatment at different levels of physiological quality with amino acids on the performance of seeds and plants in the field. The experimental design was a randomized complete block design with four replications involving a combination of two factors. Factor A consisted of seed lots of three levels of physiological quality (high, intermediate, and low) and factor B consisted of five doses of a commercial product based on amino acids used in seed treatment.Seeds of different levels of physiological quality were treated with a commercial product (C.P.) at doses of 0, 200, 400, 600, and 800 mL C.P. 100 kg−1 of seeds. After seed treatment, the physiological performance was assessed in the laboratory and then the seeds were sowed in the field aiming at assessing plant performance, yield components, and productivity. Seed treatment with amino acids does not promote the physiological performance of seeds and plants in the field. The productivity of isolated soybean plants from seed lots of a high physiological quality is 15% higher than that of plants from seed lots of a low physiological quality.

INFLUENCE OF SOYBEAN SEED MOISTURE CONTENT IN THE RESPONSE TO SEED TREATMENT IN SOYBEAN

Seed treatment (ST) is an important practice for soybean crop. This research had the objective to evaluate the influence of seed moisture content in the response to different spray volumes (SV) used for seed treatment in soybean, considering effects on seed physiological quality. Three seed lots with distinct moistures were used: 7.2%, 10.1% and 13.0%. Untreated seeds (control) and three SV were tested: 8, 13 and 18 mL kg-1. All lots received the same treatment combination, containing insecticide, fungicide, fertilizer and biostimulant. This combination represented 8 mL kg-1 of SV; the doses of 13 and 18 mL kg-1 were obtained by adding 5 and 10 mL kg-1 of water, respectively. Evaluations of seed physiological quality consisted of electrical conductivity, seed respiration, germination and vigor tests. Results of all tests demonstrates that low-moisture soybean seeds (7.2%) are negatively affected by seed treatment within an SV range of 8 to 18 mL kg-1, while untreated seeds with equal moisture are not affected. Oppositely, high-moisture seeds (13.0%) are not affected by the SV tested, while intermediate-moisture seeds (10.1%) are affected by the higher SV. This result highlights seed moisture as a key parameter to be managed before soybean ST, aiming to maintain a high physiological quality.

INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN

The paper presents the results of study on the influence of pre-sowing treatment of soybeanseeds with the microbial preparation Rhyzohumin and growth regulator Bioglobine, and their separate application (Rhyzohumin was used for seeds bacterization while growth regulator was appliedfoliar during different phases of plants organogenesis) on the formation and functioning of a symbiotic nitrogen-fixing apparatus in the conditions of the north-eastern forest-steppe of Ukraine.Separate application of studied preparations has intensively stimulated the formation and activityof soybean-rhizobial symbiosis, compared to their joint application. Considering their stage-bystage application the preparations has a positive action and increase the grain yield of soybeans.

Growth Promoting Microorganisms for Treatment of Soybean Seeds

The use of microorganisms that promote plant growth is an important tool for increasing agricultural production; besides, there is an emerging demand for decreasing utilization of chemical fertilizers and to solidify sustainable agriculture. The objective of this study was to evaluate the efficacy of growth promoting microorganisms Bradyrhizobium elkanii and Trichoderma harzianum for treatment of soybean seeds. This experiment was conducted at the Regional Institute of Rural Development (IRDeR), in Augusto Pestana, RS, Brazil. The experimental design was randomized blocks and treatments were: control, Trichoderma harzianum, Bradyrhizobium elkanii, and co-inoculated Trichoderma harzianum + Bradyrhizobium elkanii, with four replications per treatment. The sample units were five rows 5 m in length, spaced at 0.5 m, and three central lines were used to analyze the components of soybean yield. Of these, two lines were harvested 4 m, and the incidence of soil diseases in the plants was evaluated in one of the lines. Treatments means were compared using Tukey test. There was an effect of treatments: B. elkanii and treatment with co-inoculation B. elkanii and T. harzianum showed a significant response to grain yield. The chemical treatment without microorganisms with a biological effect had a higher incidence of diseases and a lower yield of grains.

Correction of soil rhizobial communities under introduction of Bradyrhizobium japonicum of different genetic groups

Biological fixation of molecular nitrogen of the atmosphere is one of the main sources of nitrogen in agrocenoses. Due to the ability to fix nitrogen, nodule bacteria are considered to be a valuable genetic resource for biotechnology in agriculture. The scale of the annual production of microbial preparations for the inoculation of legumes by the areas of crops reaches hundreds of millions of hectares. The list of biological preparations for the plant production over the past decade have expanded to include preparations based on microorganisms of various functional groups and preparations of binary action, obtained as a result of a combination of several microorganisms. The possibility of using biological preparations of several strains of rhizobia belonging to the same species has been poorly studied. The aim of our work was to study efficiency of combined application of B. japonicum strains from various genetic groups as the preparations base for soybean treatment and assessing their effect in local rhizobial soil communities. The effectiveness of B. japonicum 46 + B. japonicum KB11 binary composition as the base of Rizobofit and Rizogumin microbial preparations for soybean had been investigated. It is shown that the combination of two strains of soybean nodule bacteria from various genetic groups in biopreparations and their introduction into agrocenosis is able to correct soil rhizobial communities. Soybean seeds treatment with B. japonicum strains composition ensures the formation of balanced symbiotic systems with the participation of introduced and local rhizobia. This method allows us to intensify the process of nodule formation, increase the level of symbiotic nitrogen fixation, and increase the yields on 18–35% according to the control (without inoculation). The usage of peat after Rizogumin biopreparation was the most effective in various soil and climatic conditions.

Development and Phenotypic Screening of an Ethyl Methane Sulfonate Mutant Population in Soybean.

Soybean is an important oil-producing crop in the Fabaceae family and there are increasing demands for soybean oil and other soybean products. Genetic improvement of soybean is needed to increase its production. In order to provide genetic diversity and resources for identifying important genes, a new ethyl methane sulfonate (EMS) mutagenized soybean population was generated using the newly released germplasm, JTN-5203 (maturity group V). Treatment of soybean seeds with 60 mM EMS concentration was found to be suitable for inducing mutation. A total of 1,820 M1 individuals were produced from 15,000 treated seeds. The resulting M2 population was planted in the field for phenotyping. After harvest, seed traits including total oil, protein, starch, moisture content, fatty acid and amino acid compositions were measured by NIR. Phenotypic variations observed in this population include changes in leaf morphology, plant architecture, seed compositions, and yield. Of most interest, we identified plants with increased amounts of total protein (50% vs. 41% for control) and plants with higher amounts of total oil (25% vs. 21.2% control). Similarly, we identified plants with increases in oleic acid content and decreases in linoleic acid and linolenic acid. This EMS mutant population will be used for further studies including screening for various traits such as amino acid pathways, allergens, phytic acids, and other important soybean agronomic traits. In addition, these mutant individuals will be evaluated in the next generation to assess the heritability. Beneficial traits from these mutants can be exploited for future soybean breeding programs. This germplasm can also be used for discovering novel mutant alleles and for functional gene expression analysis using reverse genetics tools such as TILLING.

チアメトキサム・フルジオキソニル・メタラキシルＭ水和剤の種子塗布処理がダイズ品種「リュウホウ」の収量構成要素およびしわ粒に与える影響

チアメトキサム・フルジオキソニル・メタラキシルＭ水和剤の種子塗布処理がダイズ品種「リュウホウ」の収量構成要素およびしわ粒に与える影響

Composition and volumes of slurry in soybean seeds treatment in the industry and physiological quality during storage

Abstract: Seeds treatment is a relevant factor in the soybean production system. The aim of this work was to evaluate composition and volumes of slurry used in industrial treatment of soybean seeds, and their physiological quality during storage. The experimental design was completely randomized, in a 20x4 factorial scheme, i.e. 20 seeds treatments and four storage periods (0, 30, 90 and 150 days). Seeds of the cultivars NS7667IPRO and NS7709IPRO were used. The slurry composition tested were a combination of fungicide and insecticide Standak top® (Fipronil + Piraclostrobin + Thiophanate Methyl), polymer L551Incotec® and water, in order to obtain the volumes of 450, 700 and 1200 mL.100 kg of seeds-1, and the controls treatment. The physiological quality was assessed by germination in sand and accelerated aging tests. Genotypes showed distinct tolerance to seed treatment industrial , slurry volumes and storage. Treatments with Standak top®, polymer and water, with volumes of up to 1200 mL.100 kg seed-1, and stored for 150 days in cold chamber were not harmful for germination of cultivar NS7667IPRO. As for cultivar NS7709IPRO, the tolerance was 90 days. Treating seeds with large slurry volumes is detrimental to maintain seeds vigor during storage, with depreciation after 30 days, especially with water predominance.

Thermal Treatment of Soybean Seeds can Improve the Quality of Soymilk by Enhancing the Extraction Efficiency of “Kokumi” Taste Components

Based on our recent finding that the “kokumi” taste components, γ-glutamyl-tyrosine, γ-glutamyl-phenylalanine, raffinose, and stachyose, are present in soybean seeds, we aimed to understand whether thermal treatment of soybean seeds can enhance the “kokumi” taste in soymilk or not. Heating was carried out prior to the grinding step in the soymilk production process. The extraction efficiency of the “kokumi” taste components changed with the type of thermal treatment. Autoclave and microwave heating of soybean seeds before grinding caused a high extraction efficiency of “kokumi” taste components compared to unheated and dry heat heating. Traditional methods, such as the thermal treatment at 90°C during grinding, also increased the “kokumi” taste components, but the efficiency and nitrogen yield were low compared to the thermal treatment before grinding. Enhanced extraction of “kokumi” taste components may be originated from the disruption of cell walls of soybean seeds induced by thermal treatment, and the degree of cell damage was closely related with the extraction efficiency of the “kokumi” taste components. Thus, instead of the traditional processes, the thermal treatment before grinding can contribute to the production of soymilk with greater “kokumi” taste components.

Assessing the Effectiveness of Zn Acetate and Oxide as Alternatives for Corn and Soybean Seed Treatment in Sandy and Clay Soil

Zinc (Zn) is the micronutrient with the lowest availability in agricultural soils, and consequently 50 % of the world’s soils present Zn deficient. To test the viability of alternative Zn sources (Zn acetate and Zn oxide) to corn and soybean seed treatments, we ran an experiment using these two alternatives at contrasting application rates (0; 0.25; 0.50; 0.76 and 1.01 g kg-1) applied to soybean and corn seeds that were subsequently sowed in sandy and clay soils. We measured: Zn accumulation, dry matter and germination, and analyzed this data using uni (LSD-test) and multivariate analysis (Principal Component Analysis, PCA). Results of the PCA showed that the sandy soil yielded higher dry matter and Zn accumulation than the clay soil. The corn provided higher dry matter while the soybean showed enhanced Zn accumulation and germination. The LSD test showed that corn presented positive Zn accumulation in response to Zn rates in both sandy and clay soil. For soybeans, this effect was only observed in sandy soil, while the clay soil presented decreases in dry matter and germination due to Zn rates. Overall, our findings reveal that both Zn acetate and Zn oxide are viable alternatives for supplying Zn to corn seed treatment in sandy and clay soil, and to soybean seed treatment in sandy soil. We suggest that more research should be undertaken to understand the response of soybean seed treatments to Zn supply, especially in clay soil.

Effect of Soybean Cyst Nematode Resistance Source and Seed Treatment on Population Densities of Heterodera glycines, Sudden Death Syndrome, and Yield of Soybean.

A three-year study was conducted in Illinois, Indiana, Iowa, Michigan, and Ontario, Canada, from 2013 through 2015 to determine the effect of soybean (Glycine max) cultivars' source of soybean cyst nematode (SCN; Heterodera glycines) resistance on SCN population densities, sudden death syndrome (SDS; caused by Fusarium virguliforme), and yield of soybean. Five cultivars were evaluated with and without fluopyram seed treatment at each location. Cultivars with no SCN resistance had greater SDS severity, greater postharvest SCN egg counts (Pf), and lower yield than cultivars with plant introduction (PI) 548402 (Peking) and PI 88788-type of SCN resistance (P < 0.05). Cultivars with Peking-type resistance had lower Pf than those with PI 888788-type and no SCN resistance. In two locations with HG type 1.2-, cultivars with Peking-type resistance had greater foliar disease index (FDX) than cultivars with PI 88788-type. Fluopyram seed treatment reduced SDS and improved yield compared with a base seed treatment but did not affect SCN reproduction and Pf (P > 0.05). FDX and Pf were positively correlated in all three years (P < 0.01). Our results indicate that SDS severity may be influenced by SCN population density and HG type, which are important to consider when selecting cultivars for SCN management.

Joint use of fungicides, insecticides and inoculants in the treatment of soybean seeds

ABSTRACT The interference of the joint application of pesticides with seed inoculation on the survival of Bradyrhizobium has been reported in the last years. So, the objective of this study was to evaluate the joint use of fungicides, insecticides and inoculant in the treatment of soybean seeds on various parameters of Bradyrhizobium nodulation in soybean as well as on crop productivity parameters. The experiment was conducted during the 2013/2014 crop in the experimental field of the Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso - Campo Novo do Parecis Campus. The seeds of TMG 133 RR variety were sown in pots. It was used a randomized block design in a 4 x 4 + 1 factorial, four fungicides (1: fludioxonil + metalaxyl-M, 2: carboxine + thiram, 3: difeconazole and 4: carbendazim + thiram), four insecticides (1: fipronil 250 SC, 2: thiamethoxam, 3: imidacloprid + thiodicarpe and 4: imodacloprid 600 FC) and an inoculant (SEMIA 5079 and SEMIA 5080), common to all treatments, with three replications. The experiment was not repeated. The joint application of fungicide and insecticide with inoculant does not affect nodulation, foliar N content and vegetative growth of the plants as well as the masses of grains per plant and 100-grain mass. The use of the carbendazim + thiram mixed with fipronil and carbendazim + thiram mixed with imidacloprid provides less number of pods per plant and grains per plant, reflecting in reductions in the production of soybean grains. In this way, the fungicide carbendazim + thiram, regardless of the combined applied insecticide, is the most harmful to Bradyrhizobium spp.

Soybean Seed Treatment with Nickel Improves Biological Nitrogen Fixation and Urease Activity

Nickel (Ni) is an essential micronutrient required for plants’ metabolism due to its role as a structural component of urease and hydrogenase, which in turn perform nitrogen (N) metabolism in many legume species. Seed treatment with cobalt, molybdenum and Bradyrhizobium strains has been widely practiced to improve crops. Additionally, seed treatment together with Ni fertilization of soybean might improve the efficiency of biological nitrogen fixation (BNF), boosting grain dry matter yield and N content. The objective of this study was to evaluate the effect of soybean seed treatment with Ni rates (0, 45, 90,135, 180, 360 and 540 mg kg-1) on biological nitrogen fixation (BNF), directly by the 15N natural abundance method (δ15N‰) and by measurement of urease [E.C. 3.5.1.5] activity, as well as indirectly by nitrogenase (N-ase) activity [E.C. 1.18.6.1]. Soybean plants (cultivar BMX Potencia RR) were grown in a sandy soil up to the R7 developmental stage (grain maturity), at which point the nutrient content in the leaves, chlorophyll content, urease and N-ase activities, Ni and N content in the grains, nodulation (at R1 - flowering stage), as well as the contribution of biological nitrogen fixation (δ15N ‰), were evaluated. The proportion of N derived from N2 fixation varied from 77 to 99% using the natural 15N abundance method and non-nodulating Panicum miliaceum and Phalaris canariensis as references. A Ni rate of 45 mg kg-1 increased BNF by 12% compared to the control. The increased N uptake in the grains was closely correlated with chlorophyll content in the leaves, urease and N-ase activities, as well as with nodulation. Grain dry matter yield and aerial part dry matter yield increased, respectively, by 84% and 51% in relation to the control plants at 45 mg kg-1 Ni via seed treatment. Despite, Ni concentration was increased with Ni-seed treatment, Ni rates higher than 135 mg kg-1 promoted negative effects on plant growth and yield. In these experimental conditions, seed treatment with low Ni rates caused higher dry matter yield of plants and grains.

Elucidation of the role of oleosin in off-flavour generation in soymeal through supercritical CO2 and biotic elicitor treatments

Defatting soybean by sophisticated oil extraction method utilising supercritical CO2 resulted in a significant decrease in the residual phospholipids (PLs) compared with soymeal obtained by conventional cold percolation method utilising hexane as the extraction solvent. Interestingly, the levels of residual PLs showed a proportionate relationship with thiobarbituric acid (TBA) number, an indicator of lipid peroxidation responsible for off-flavour generation. Furthermore, two oleosins (18 and 24kDa) were isolated from the oil bodies extracted from soybean seeds and positively characterised for phospholipase A2 (PLA2) activity, suggesting their plausible involvement in off-flavour generation in soymeal. The treatment of soybean seeds, before oil extraction, with different concentrations of biotic elicitors such as chitosan and jasmonic acid also significantly reduced the levels of residual PLs as well as the TBA number. The biotic elicitor treatment could thus prove to be an important strategy for the reduction of off-flavour in protein-rich soymeal.

Seeds treatment times in the establishment and yield performance of soybean crops

Abstract: The objective was to assess the early treatment effect of soybean seeds and pre-sowing with different combinations of chemicals on the establishment of plants and crop yield performance. The design was in randomized blocks in a 2x7 factorial arrangement, with two times for seed treatment and seven treatments (six chemical treatments and an untreated control). The treatments were: 1) fipronil + pyraclostrobin + thiophanate methyl; 2) imidacloprid + thiodicarb + carbendazin + thiram; 3) abamectin + thiamethoxam + fludioxonil + mefenoxam + thiabendazole; 4) carbendazin + thiram; 5) fludioxonil + mefenoxam + thiabendazole; 6) carboxin + thiram; and 7) untreated control (water only). The assessments were: seedling emergence, final stand, plant height and insertion of first pod, number of pods per plant, seeds per pod and per plant, thousand-seed weight and grain yield. Early treatment of soybean seeds (240 days prior to sowing) hinders the establishment of the crop, the thousand-seed weight and grain yield in relation to the pre-sowing treatment. Chemical treatments tested containing fungicides and insecticides associated favor the establishment of the crop, but do not alter the soybean yield performance.

Effects of Streptomyces &amp;lt;i&amp;gt;Biofertilizer&amp;lt;/i&amp;gt; to Soil Fertility and Rhizosphere’s Functional Biodiversity of Agricultural Plants

In the present study, a biofertilizer on the basis of Streptomyces fumanus gn-2 was used for the treatment of wheat and soybean seeds (dose 104 spore/ml) before planting them in soil with low fertility in order to determine the effect of this biological agent on germination rate; the growth of seedlings, shoots, and the maturation phase of plants; the rhizosphere’s functional biodiversity; and the resistance of these plants to pathogens. Seeds were soaked in the suspension for a period of two or three hours. During the growing season of the crop, no additional fertilizing and spraying of a biopesticide against diseases or pests occurred. Despite the soil having low fertility, low quantities of organic matter, and not having been before used for the cultivation of agricultural plants, this biofertilizer showed a strong stimulatory effect on the growth of seeds and seedlings of wheat and soybeans. The average germination and seed vigor increased by 1.5 - 2.0 times, and the phenophases were accelerated to three to five days. In all phases of vegetation, the ammonifying bacteria in the presence of an antagonist (a biological agent) developed rapidly and were constantly present in significant numbers in the rhizosphere. Streptomyces fumanus introduced into non-sterile soil entered into competition with the local soil microflora and had the ability to colonize the rhizosphere system of plants. The use of a formulation of Streptomyces gn-2 has improved the composition of rhizosphere microflora, attracting saprophytic microorganisms: ammonificators and oligotrophs. The presence of the biocontrol microorganism Streptomyces fumanus in the rhizosphere plays an important role in enhancing the growth and development of useful groups, such as nitrogen-fixing bacteria.