Levels Of Soybean Meal Research Articles

PSI-A-11 The Effect of Soybean Meal and Its Functional Compounds on Growth Performance, Fecal Scores, and Fecal Characteristics of Nursery Pigs

Abstract High levels of soybean meal (SBM) are deterred in nursery diets due to antinutritional factors and impacts on fecal consistency. However, SBM may improve performance in the presence of inflammatory stressors due to its functional compounds. The experimental objective was to evaluate the non-nutritive value of SBM and its functional compounds on growth performance and fecal characteristics of nursery pigs. A total of 408 newly weaned pigs with an initial BW of 5.43 ±0.77 kg were used for a 35-d study across three replications. Pigs were randomly assigned to 1 of 6 dietary treatments fed across 3 phases: d 0-7 (phase 1), d 7-21 (phase 2), and d 21-35 (phase 3). Dietary treatments consisted of a high SBM control (SBMC), a control void of SBM (SP), but included soy protein isolate and concentrate to provide amino acids similar to SBM in SBMC; SP formulated to equivalent contribution of isoflavones as SBMC (SP+ISO); SP diet fortified with soybean functional lipids to the level of SBMC (SP+LIP); SP formulated with a similar ratio of insoluble to soluble fiber as SBMC (SP+FIB); or SP diet fortified with lunasin (SP+LUN). There were 9 split-sex pens per treatment (7 or 8 pigs/pen). On d 7, 14, 21, and 35, pigs and feeders were weighed, pen fecal samples were collected, and fecal score was evaluated using a 4-point scale (1 = solid; 4 = liquid). Continuous data were analyzed as a linear mixed model (PROC MIXED) with treatment and period as fixed effects, replicate as a random effect, and initial BW as a covariate where appropriate. Fecal scores were analyzed using a multinomial model in PROC FREQ. Compared with SP, on d 7 and 14 pigs fed SBMC and SP+FIB had reduced fecal dry matter (DM%), but on d 21 and 35, fecal DM% did not differ among treatments (time×treatment P = 0.016). There was a treatment×time interaction for fecal score, whereas on d 7, pigs fed SBMC had increased proportion of semi-liquid feces and on d 14 pigs fed SP+FIB had increased abundance of semi-liquid feces, but fecal score distribution did not differ on d 21 or 35 (time×treatment P = 0.035). Fecal water binding capacity (WBC) did not differ across time (P=0.288) but relative to SP, SBMC had 30% less fecal WBC (P = 0.002). There was a treatment×time interaction for ADG and ADFI. Pigs fed SP+ISO had greater ADG in phase 2, but pigs fed SBMC gained more in phase 3 (time×treatment P = 0.044). Among treatments, ADFI did not differ in phase 1 and 2, but was increased in SBMC in phase 3 (time×treatment; P = 0.037). Pigs fed SBMC tended to have the greatest final BW (P = 0.071). Feeding SBM altered fecal characteristics in the early nursery period, but improved growth performance from d 21-35 post-weaning.

40 Effect of Soybean Meal Inclusion on Grow-Finish Pig Performance and Nitrogen Balance

Abstract Mixed-sex pigs (n = 240 PIC 337×1050; initial BW = 23 ± 4.7 kg) were used to evaluate the effects of soybean meal (SBM) inclusion on the growth performance and nitrogen balance of grow-finish pigs. Pigs were assigned across 60 split-sex pens, and pens were randomly assigned to one of three dietary treatments (10 pens∙treatments-1∙sex-1). Diets were formulated with increasing SBM for an approximate 3% incremental difference in crude protein (CP) between treatments. The treatments were: 1) low SBM (L-SBM) corn-soy diet supplemented with synthetic amino acids, 2) medium SBM diet (M-SBM) with SBM formulated to replace all synthetic lysine and equal to L-SBM in SID lysine, and 3) high SBM diet (H-SBM) contained excess levels of all amino acids. Diets were fed in four feeding phases ranging from approximately 25-50, 51-75, 76-105, and 106-135 kg for phases 1-4, respectively. From phases 1 to 4, SBM inclusion ranged from approximately 26-48% (SID Lys 1.06-1.32%), 19-41% (SID Lys 0.89-1.15%), 15-35% (SID Lys 0.76-1.01%), and 13-29% (SID Lys 0.68-0.86%), respectively. Additionally, diets within each phase were isocaloric. Pig BW and feed intake were measured for each phase to calculate ADG, ADFI, and G:F. On day 66, 18 gilts (n =6/treatment) were placed into metabolism stalls for total urine and feces collection over a 72 h period to assess CP digestibility and nitrogen balance. Pigs were marketed at approximately 134 kg BW, and carcass weight, backfat depth, and loin depth were measured at the harvest facility. Data were subject to ANOVA in SAS 9.4. Pen was the experimental unit, and the results were significant at P ≤ 0.05. Overall (d 0-98) pig ADFI, ADG, or G:F did not differ across treatments (P ≥ 0.202). However, there was a significant diet-by-phase interaction for ADG (P = 0.045) and G:F (P = 0.008), where H-SBM fed pigs grew faster in phase two compared with the M-SBM (1.07 vs. 1.02 kg/d; P = 0.026) or L-SBM (1.07 vs. 1.02 kg/d; P = 0.028) fed pigs. Consequently, in phase two, H-SBM pigs had improved G:F compared with M-SBM (0.47 vs. 0.44; P = 0.005) or L-SBM pigs (0.47 vs. 0.44; P = 0.001). However, these effects were not evident in the other phases. Apparent total tract digestibility of CP and nitrogen retention were similar between treatments (P ≥ 0.762). However, as expected, nitrogen retention as a proportion of intake, linearly decreased (P < 0.001) as SBM inclusion increased, which translated to an approximate 130% increase in total nitrogen excretion in pigs fed H-SBM compared with L-SBM (40.8 vs. 17.7 g/d; P < 0.001). In conclusion, low and high SBM formulations did not alter grow-finish pig overall performance. However, linearly increased nitrogen excretion was reported as SBM levels increased.

120 Evaluation of Anchovy Fish Meal and Fish Solubles Compared with Other Specialty Protein Sources on Nursery Pig Performance

Abstract A total of 330 pigs (DNA 600 × 241, initially 4.9 ± 0.2 kg, 19 d old) were used to evaluate anchovy fish meal and fish solubles compared with other specialty protein sources on nursery pig growth performance and fecal dry matter (DM). At weaning, pigs were allotted to 1 of 6 dietary treatments with 4 or 5 pigs per pen and 12 replications per treatment in a completely randomized design. Dietary treatments consisted of different protein sources added based on relative SID Lys content and included: 1) enzymatically treated soybean meal (ESBM, HP 300, Hamlet Protein, Findlay, OH) at 7.0% of the diet; 2) spray-dried bovine plasma (SDBP, APC Corp, Ankeny, IA) at 3.5% of the diet; 3) fermented soybean meal (ME-PRO, Prairie Aquatech, Brookings, SD) at 5.0% of the diet; 4) fermented soybean meal enriched with 6.5% fish solubles (TASA, Lima, Peru) at 5.21% of the diet; 5) fish meal (TASA Prime meal, TASA, Lima, Peru) at 4.85% of the diet; and 6) fish meal enriched with 6.5% fish solubles (TASA Swine, TASA, Lima, Peru) at 5.05% of the diet. All diets contained the same conventional soybean meal level within phase. Treatment diets were fed in 2 phases, d 0 to 12 and d 12 to 25, for phase 1 and 2, respectively. Following phase 2, all pigs were fed a common diet for an additional 15 d. Although there was a 5 to 7% improvement in ADG for pigs fed spray-dried bovine plasma and fish meal with solubles, there were no significant differences in growth performance (P > 0.10) between treatments for any period or overall (Table). There was a treatment × day interaction (P = 0.003) for fecal dry matter largely driven by the increase in fecal DM for pigs fed spray-dried bovine plasma from d 12 to 25. On d 12, there were no differences between treatments for fecal DM (P > 0.10); however, on d 25, pigs fed spray-dried bovine plasma had improved (P = 0.017) fecal DM compared with pigs fed enzymatically treated soybean meal with all other treatments intermediate. In summary, anchovy fish meal with or without fish solubles can be utilized in nursery pig diets without decreasing growth performance.

Efficiency of the use of compound foodstuffs with different levels of formulated fermented soya meal EP500 when growing African catfish (Clarias gariepinus) to market weight

The article highlights the influence of feeding Clarias gariepinus with mixed fodders with different levels of replacement of fish meal with fermented soybean meal EP500 on the live weight and average daily weight gain of Clarias gariepinus reared to marketable weight. Experimental studies were conducted at the Pshenychnyi Department of Animal Nutrition and Feed Technology of the National University of Life and Environmental Sciences of Ukraine. A scientific and economic experiment was conducted using the method of analogue groups lasting 84 days, which was divided into 6 subperiods lasting 14 days each. For the experiment, 300 heads of African sharptooth catfish with an average weight of 351–352 grams were selected and formed by the method of analogues into three groups of 100 heads each — control and 2 experimental. The fish were fed with compound feed, which differed in different levels of fermented soybean meal. Thus, in the feed of the control group, the amount of fish meal was 36% without the addition of fermented soybean meal EP500, while in the feed of group 2, the content of fish meal was reduced to 11% and 25% of fermented soybean meal EP500 was added. In the preparation of feed for group 3, efforts were made to replace 100% of fish meal with fermented soybean meal EP500, while the content of fermented soybean meal EP500 was 36%. On the 56th day of the experiment, changes in live weight became significant. Thus, the third group, which contained 36% of fermented soybean meal EP500, was 5.22 g (p≤0.05) ahead of the control in live weight. The second group was heavier than the control by 3.68 g, but there was no significant difference between them. When weighed on day 70 of the experiment, the live weight in the second experimental group was 9.34 g higher than in the control group (p≤0.05) and became significant. At the same time, the third group was 10.9 g higher in live weight compared to the control group (p≤0.05) and continued to be significant. At the end of the experiment on day 84, this pattern continued. Thus, the live weight of African sharptooth catfish in the second and third experimental groups was higher compared to the control by 9.77 g (p≤0.05) in the second experimental group and 12.07 g (p≤0.01) in the third experimental group, although there was no statistical significance between the experimental (second and third) groups. The analysis of the average daily weight gain for the entire period showed a significant difference in this indicator in the second and third groups, which exceeded the control by 1.34% (p≤0.01) and 1.57% (p≤0.001), respectively.

Screening and Stability Analysis of Reference Genes for Gene Expression Normalization in Hybrid Yellow Catfish (Pelteobagrus fulvidraco ♀ × Pelteobagrus vachelli ♂) Fed Diets Containing Different Soybean Meal Levels.

In this study, we screened the expression stability of six reference genes (18S rRNA, β-actin, GAPDH, EF1a, B2M, and HPRT1) in hybrid yellow catfish (n = 6), considering the SBM levels, sampling time points, and different tissues. Four different statistical programs, BestKeeper, NormFinder, Genorm, and Delta Ct, combined with a method that comprehensively considered all results, were used to evaluate the expression stability of these reference genes systematically. The results showed that SBM levels significantly impacted the expression stability of most of the reference genes studied and that this impact was time-, dose-, and tissue-dependent. The expression stability of these six reference genes varied depending on tissue, sampling time point, and SBM dosage. Additionally, more variations were found among different tissues than among different SBM levels or sampling time points. Due to its high expression, 18S rRNA was excluded from the list of candidate reference genes. β-actin and GAPDH in the liver and β-actin, HPRT1 and EF1a in the intestine were the most stable reference genes when SBM levels were considered. HPRT1, and EF1a in tissues sampled at 2 W and EF1a and β-actin in tissues sampled at 4 and 6 W were proposed as two stable reference genes when different tissues were considered. When the sampling time points were considered, β-actin, EF1a, and HPRT1 were the top three stable reference genes in the intestine. In contrast, β-actin and B2M are the most stable reference genes in the liver. In summary, β-actin, EF1a, and HPRT1 were the more stable reference genes in this study. The stability of reference genes depends on the tissues, sampling time points, and SBM diet levels in hybrid yellow catfish. Therefore, attention should be paid to these factors before selecting suitable reference genes for normalizing the target genes.

Comparative evaluation of soybean meal vs. extruded soybean meal as a replacer for fishmeal in diets of olive flounder (Paralichthys olivaceus): Effects on growth performance and muscle quality

A 56-day feeding trial was performed to compare the impacts of replacement of dietary fishmeal by soybean meal (SM) or extruded soybean meal (ESM) on the growth performance and muscle quality of olive flounder (Paralichthys olivaceus) (initial weight: 6.32 ± 0.01 g). Nine isonitrogenous and isolipidic diets were prepared by substituting 0% (FM, control), 12% (SM12/ESM12), 24% (SM24/ESM24), 36% (SM36/ESM36) and 48% (SM48/ESM48) of fishmeal with SM or ESM respectively. Results indicated that contents of anti-nutritional factors (trypsin inhibitors, glycinin and β-conglycinin) in ESM were significantly lower than those in SM. The weight gain rate, specific growth rate, feed efficiency ratio, protein efficiency ratio, activities of intestinal trypsin and lipase, as well as expressions of myogenic regulatory factors (myf5, myod and mrf4) and protein synthesis-related genes (akt, pi3k, tor and s6) in fish muscle decreased linearly and quadratically with increasing replacement levels of dietary fishmeal by SM or ESM. Expressions of mstn and protein degradation-related genes (foxo1, murf-1, mafbx, capn2 and ctsl) in muscle showed a trend opposite to growth parameters responding to dietary SM or ESM levels. Significant differences in all the above parameters were observed in the SM36, SM48 and ESM48 groups, accompanied by the declines in contents of most muscle free amino acids. The increase of dietary fishmeal replacement level by SM linearly and quadratically decreased muscle hardness, water holding capacity (WHC), pH and total collagen content. Markedly lower values were found in the SM36 and SM48 groups. However, ESM could replace up to 36% of dietary fishmeal without significantly impacts on above muscle quality parameters. Additionally, reduced myofiber density and sarcomere length were observed in the SM36, SM48, ESM36 and ESM48 groups. However, at the same substitution level, dietary ESM relieved the effects of SM on myofiber morphology to some extent. In conclusion, according to broken-line regression on SGR, the optimal replacing level of fishmeal by SM and ESM for olive flounder was 22.07% and 34.39% respectively. The improved replacement level of fishmeal by ESM, in terms of fish growth, muscle hardness and WHC, may be due to the fact that dietary ESM alleviated the adverse impacts of SM on digestive enzyme activity, muscle growth-related genes expression, muscle pH, collagen content and myofiber morphology of fish.

Evidence of enteritis, hepatic steatosis and jaundice in juvenile pirarucu (Arapaima gigas) fed high levels of soybean meal

ABSTRACT This study evaluated the effects of replacing dietary fishmeal with increasing levels of soybean meal (0, 15, 30, 45 and 60% corresponding to 0, 11, 22, 33 and 44% inclusion in the formulated diet) on the intestine and liver histomorphology of juvenile pirarucu Arapaima gigas (233 ± 12 g; mean ± SD) fed for 120 days. At the end of the feeding trial, fish fed diets with higher soybean levels (45 and 60%) presented clinical signs such as appetite loss, cachexia and jaundice. In addition, fish fed on 45% and 60% soybean meal had histomorphological alterations in the middle and posterior portions of the intestinal mucosa (enteritis) and high liver lipid deposition (steatosis). Therefore, 45% or 60% of soybean meal is not recommended; however, a replacement of fishmeal by soybean meal of up to 30% (22% inclusion) can be used in diets to feed juvenile pirarucu for long periods.

Effects of tributyrin on the growth performance, survival, tolerance to hypoxic stress, and nutrient digestibility of Pacific white shrimp, Litopenaeus vannamei, fed different levels of soybean meal

AbstractThe study aimed to evaluate the effect of tributyrin on the growth performance, survival, hypoxia tolerance, and nutrient digestibility of Pacific white shrimp fed 25% and 50% soybean meal (SBM) diets. Six experimental diets were formulated: (1) 25% SBM without tributyrin; (2) 25% SBM with 0.05% tributyrin; (3) 25% SBM with 0.1% tributyrin; (4) 50% SBM without tributyrin; (5) 50% SBM with 0.05% tributyrin; and (6) 50% SBM with 0.1% tributyrin. The shrimp were fed the experimental diets daily for 10 weeks. Tributyrin supplementation at 0.1% diet significantly improved the weight gain of shrimp that fed 25% SBM but not the other groups. Nevertheless, shrimp that fed tributyrin had significant improvement in survival rate at both SBM levels. Significant reductions in Vibrio spp. counts in the hepatopancreas and intestine were also evident in all tributyrin‐fed groups. In addition, 0.1% tributyrin in both SBM levels significantly enhanced the shrimp tolerance to hypoxia. Lastly, the digestibilities were generally unaffected by the tributyrin except only for the lipid digestibility of the 0.1% tributyrin in 25% SBM group, which showed a significant increase. Overall, tributyrin was proven beneficial for the shrimp's health, especially for those fed diet with a moderate level of SBM (25%).

Impact of dietary soybean inclusion on the endocrine disruptive effect in female brood stock of Cyprinus carpio

Phytoestrogens, mostly found in legumes, mimic animal estrogens and induce either pro- or anti-estrogenic activity. A feeding trial for matured Cyprinus carpio females was carried out to assess the endocrine disrupting effect of phytoestrogens in SBM and to suggest a dietary inclusion of soybean meal for broodstock of carps. The fish were fed diets containing graded levels of soybean meal (SBM-0, 15, 20, 25, 30, 35, and 40%) for 60 days. To assess the endocrine disruption, sex hormone profiling, gene expression analysis of candidate genes, and histological examinations were conducted. Serum T3, serum vitellogenin, and mRNA levels of cyp19a1, cyp19b, and erβ were found to be higher in fish groups fed with diet containing low levels of SBM (SBM 15, 20, and 25), and significantly lower in high SBM diet (≥30%) fed groups. Serum concentrations of cholesterol, cortisol, and the mRNA level of 20βHSD all decreased as SBM increased. Increased levels of testosterone, estradiol, and the mRNA for the erα gene were observed in the diet when SBM was incorporated into the diet. In the treatment groups with ≥30% inclusion of SBM, the number of vitellogenic oocytes and the gonadosomatic index were significantly lower than the groups with low inclusion levels. Thus, soybean meal can be included in the diet of female carp broodstocks up to 25% without disrupting the reproductive function. However, an inclusion level beyond 25% could harm the endocrine axis, subsequently capable of impacting reproductive performance.

Tryptophan can alleviate the inhibition in growth and immunity of tilapia (GIFT Oreochromis spp.) induced by high dietary soybean meal level

A 85-day feeding trial was designed to verify the effects of soybean meal on growth, feed utilization, antioxidants, and immune response of tilapia (GIFT Oreochromis spp.), and evaluated the effects of dietary tryptophan on alleviating the negative responses induced by high inclusion of soybean meal. Five experimental diets, two of which substituted 15 % and 45 % of the fish meal with soybean meal, the others supplemented with 0.2 %, 0.4 % and 0.6 % L-tryptophan on the basis of 45 % soybean meal, respectively, were fed 400 juvenile fish (initial weight: 2.98 ± 0.04 g). The results showed that the crude ash content, red blood cell count (RBC), lipase (LPS) and glutathione peroxidase (GSH-Px) activities of tilapia significantly decreased when the soybean meal replaced fish meal by 45 % (p < 0.05). Although the growth performance values of replacing 45 % soybean meal were slightly lower than that of replacing 15 % soybean meal, while there was no significant difference between them (p > 0.05). However, supplementation with tryptophan in the high soybean meal replacement groups significantly improved the final weight, weight gain rate (WGR), specific growth rate (SGR), phagocytosis index (PI), RBC, trypsin (TPS), LPS and GSH-Px activities of tilapia (p < 0.05), and the apparent digestibility and feed coefficient of tilapia were significantly decreased (p < 0.05). Taken together, these results suggested that excessive soybean meal inhibited the feed utilization and immunity of tilapia to a certain extent, while the inclusion of 0.2 %−0.4 % tryptophan in the diet could alleviate the negative effects of high soybean meal diet in tilapia. This study will provide ideas and a theoretical basis for further expanding the application of soybean meal in tilapia feed.

Thermal degradation kinetics of high‐performance urea‐formaldehyde resin prepared by replacing partial urea with soybean processing residue

AbstractIn this work, soybean meal was applied to replace partial urea to prepare high‐performance urea‐formaldehyde resin (SMUF). The optimum addition amount of soybean meal was screened by the basic properties of resin, the bonding strength and formaldehyde emission of the board. The activation energy (Ea) and reaction kinetics at different conversion rates were estimated by Kissinger and Flynn–Wall–Ozawa methods. The results showed that when 5 wt% soybean meal was used to replace urea, the formaldehyde emission of plywood decreased by 19.23% and the bonding strength increased by 36.29%, which were 0.84 mg L−1 and 1.69 MPa, respectively. Meanwhile, the thermal stability of Urea‐formaldehyde (UF) resin is improved. The thermal degradation Ea of SMUF is higher than that of UF, the reaction kinetic equation of both UF and SMUF are G(α) = [−ln (1 − α)]2. It is feasible to replace partial urea with soybean meal. When the addition level of soybean meal is 5 wt%, SMUF has better comprehensive performance. When the addition level of soybean meal is 10–20 wt%, the bonding strength of plywood is higher than that of UF, but the formaldehyde emission is higher. How to reduce the formaldehyde emission will be the direction of future research.

Replacement of Dietary Crude Protein of Soybean Meal by Tobacco Leaf Protein Concentrate on Growth, Haematological and Biochemical Responses of Catfish, Clarias gariepinus

This study assessed the effects of replacing dietary crude protein of soybean meal with tobacco leaf protein concentrate (TLPC) on growth, haematological and biochemical parameters of catfish, Clarias gariepinus juveniles. The catfish (n=126, mean weight = 31.77±0.07g) were arranged in a completely randomized design with 6 treatments and 3 replicates with 7fish per experimental unit and fed for 40days. The treatments were 0, 20, 40, 60, 80 and 100% replacement levels of soybean meal with Tobacco leaf protein concentrate respectively. Diets had negative effects (p &gt; 0.05) on mean weight gain (MWG), specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) across treatments. Fish in the control group recorded highest values for MWG (51.20±0.25g), SGR (2.40±0.01) and PER (2.48±0.02). Similarly, the best value for FCR (1.03±0.01) was recorded amongst the control group of fish. With the exceptions of WBC, NEUT, LYPH and MCV, significant differences (p&lt;0.05) were recorded in the values of Packed cell volume, red blood cells, Haemoglobin, PLT, MCHC and MCH across diets. The biochemical parameters recorded no significant differences (p &gt; 0.05) across treatments with the exceptions of CHO, HDL and LDL. Also, significant differences (p &lt; 0.05) were observed in the values of GSH, SOD, CAT and MDA across treatments. The poor growth performance coupled with reduced physiological activities of TLPC at different inclusion levels, confirmed that it could not favourably replace soybean meal however, TLPC enhanced the enzymatic antioxidant activities in the experimental fish, consequently improving the health status of C. gariepinus.

Dietary supplementation with soybean oil has not favored the tilapia growth in BFT tanks submitted to feeding restriction

The present work aimed at assessing the possibility of compensating the notorious deficiencies of bioflocs in lipids by supplementing the tilapia commercial diet with soybean oil. In the positive control, there was no feeding restriction nor dietary supplementation with soybean oil. In the experimental treatments, the commercial diet was restricted by 25% over the positive control level. In the negative control tanks, there was feeding restriction and the artificial diet had no oil supplementation. In the experimental tanks, soybean oil was mixed daily with the commercial diet at the levels of 0.6%, 1.2 and 2.4%. Additionally, there were fed-restricted tanks that received a daily supplementation of 1.2% soybean oil mixed with dry molasses, and not with the commercial diet. In general, only the restriction of the commercial diet affected the water quality. The supplementation of the artificial diet with soybean oil up to 2.4% has not improved the proximate composition of bioflocs, nor the fish growth performance. It was concluded that the strategy of supplementing the Nile tilapia juveniles’ commercial diet with increasing levels of soybean meal, in BFT tanks submitted to 25%-feeding restriction, was not capable of avoiding the fish growth performance deterioration.

Effects of Clostridium butyricum and sodium butyrate on growth performance, immunity, and gut microbiota of mirror carp Cyprinus carpio fed with soybean meal based diet

This study investigated the effects of Clostridium butyricum (CB) and sodium butyrate (SB) on the growth performance, intestinal immune, and gut microbiota of mirror carp (Cyprinus carpio) fed with a high level of soybean meal. Three hundred sixty healthy carp fingerlings (∼3.74 g) were divided into eight dietary treatments with four replicates and ten fish per replicate. Which include basal diet (Control group, C0); C0 supplemented with three levels of CB, 3 × 107 cfu/kg (C1), 3 × 108 cfu/kg (C2), 3 × 109 cfu/kg (C3); three levels of SB, 500 mg/kg (S1), 1000 mg/kg (S2), 2 000 mg/kg (S3); and 3 × 108 cfu/kg CB+ 1 000 mg/kg SB (CS), respectively. In 8-week trial, the survival rate of all groups was 100%. Compared with C0, the Weight Gain, Feed Conversion Ratio, Protein Efficiency Ratio were not significantly different in all groups (P > 0.05). Crude protein and ether extract contents were increased significantly (P < 0.05) when supplemented with different levels of CB and SB, and ash content increased significantly in the CB groups (P < 0.05). Serum globulin increased and glucose decreased significantly (P < 0.05) in the CB and SB groups. The intestinal T-SOD and glutathione activity in the foregut, midgut, and hindgut were increased (P < 0.05), and villus height of foregut and midgut were higher in the CB and SB (P < 0.05). Protease activities in the foregut and midgut were significantly increased (P < 0.05) in the C2, C3, S2, and S3. The lipase activity of the C2 and C3 in foregut, midgut, and hindgut; S2, S3, and CS in the foregut; S3 in midgut were significantly increased (P < 0.05). The gut microbiota was modulated at the phylum and genera level by CB and SB administration. In summary, our results indicated that CB and SB could promote the whole body CP and EE content, intestine immunity, digestive enzyme activity, and intestinal morphology, and modulated gut flora in carp.

Effects of Increasing Levels of Soybean Meal in Nursery Diets on Growth Performance and Fecal Characteristics of 22- to 60-lb Pigs

Effects of Increasing Levels of Soybean Meal in Nursery Diets on Growth Performance and Fecal Characteristics of 22- to 60-lb Pigs

Sea lettuce (Ulva lactuca) meal combined with soybean meal in a low fishmeal diet enhanced the growth and feed intake of cultured golden rabbitfish (Siganus guttatus) during the nursery stage

A preliminary feeding trial was conducted to evaluate the effect of utilizing a high level of sea lettuce (SL), Ulva lactuca meal, as a dietary ingredient on the growth and biochemical composition of the golden rabbitfish, Siganus guttatus. Two treatments included one diet containing high SL meal combined with soybean (PP) and another diet that used fish meal as the primary protein source (FM). Golden rabbitfish were stocked into 8 of 120 L fiberglass tanks with a density of 20 fish/tank and fed for 15 weeks. The weight gain of the fish-fed PP diet was 453% higher than those provided with FM (343%). Similarly, the SGR of fish fed the PP diet was higher (1.40 %/d) compared to the PF diet (1.22 %/d). The FCR of the FM diet was lower (1.3) compared to the PP diet (1.6). Fish fed the FM diet contained a higher ash content than those fed the PP diet (14.9 vs 10.1%). Crude protein content in body fish was relatively similar for both diets. Including sea lettuce meal at 30% combined with a high level of soybean meal in a low fishmeal diet, they produced better growth performances of rabbitfish during the nursery stage.

Effects of increasing soybean meal in corn-based diets on the growth performance of late finishing pigs.

Three experiments were conducted to determine the effects of increasing soybean meal (SBM) levels by replacing feed-grade amino acids (AA) in corn, corn dried distillers grains with solubles (DDGS), and corn-wheat midds-based diets on growth performance of late finishing pigs (n = 4,406) raised in commercial facilities. Across all experiments, pens of pigs were blocked by initial bodyweight (BW) and randomly assigned to 1 of 5 dietary treatments. All diets were formulated to contain 0.70% standardized ileal digestible (SID) Lys and varying amounts of feed-grade AA. All diets were formulated to meet or exceed minimum essential AA requirement estimates as a ratio to Lys. In Exp. 1, 1,793 pigs (initially 104.9 ± 4.9 kg) were fed corn-based diets and pens of pigs were assigned treatments with increasing SBM from 5% to 20%. Overall, average daily gain (ADG) and feed efficiency (G:F) improved (linear and cubic, P ≤ 0.02) as dietary SBM increased, with the greatest improvement observed as SBM increased from 5% to 8.75% and little improvement thereafter. In Exp. 2, 1,827 pigs (initially 97.9 ± 4.3 kg) were fed diets containing 25% DDGS with SBM levels increasing from 0% to 16%. Overall, feed efficiency marginally improved (linear, P ≤ 0.10) as SBM increased, with the greatest performance observed when diets contained 8% SBM and similar performance thereafter with 12 or 16% dietary SBM. In Exp. 3, 786 pigs (initially 96.7 ± 3.2 kg) were fed diets that contained 30% wheat midds and dietary SBM from 0% to 16%. Final BW of pigs increased (linear, P < 0.05) and overall ADG and G:F improved (linear and cubic, P < 0.05) as SBM increased. The combined results of the three experiments suggest that inclusion of at least 4% to 8% dietary SBM at the expense of feed-grade amino acids in corn-based diets with or without grain coproducts can improve growth performance of late-finishing (greater than 100 kg) pigs.

Replacing of soybean meal with sunflower meal with and without multi-enzyme on laying performance and egg quality in Hy-Line laying hens

This experiment was performed to evaluate the effects of replacement of different levels of soybean meal with sunflower meal on laying performance and egg quality parameters in laying hens. In this experiment, 360 laying hens from the age of 47 to 57 weeks, in 9 treatments and 5 replications (8 hens in each replication) were divided into a 3 × 3 factorial arrangement consisting of sunflower meal (0, 30, and 60%) and enzyme additive (0, 100 and 200 g/ton) in a completely randomized design for ten weeks. The results showed that the replacement of soybean meal with sunflower meal and enzyme has significant effects on the performance of laying hens. So that 60% replacement of soybean meal with sunflower meal increased the amount of daily feed intake of laying hens (P&lt;.0.05). The use of 200 gr/ton of multi enzyme in laying hens diet, without having significant effects on other performance parameters, increased the amount of daily feed intake (P&lt;.0.05). Concurrently, there was a non-significant (P&gt;.0.05) trend for egg quality parameters, but weights of egg albumin were affected significantly (P&lt;.0.05) substitution of 60% of sunflower meal in diets increased the amount egg albumin weight. In general, in laying hens, it is possible to replace 60% of soybean meal with sunflower meal without adversely affecting the performance and egg quality. The use of a multi-enzyme only increased the amount of feed intake.

Replacing soybean meal with sunflower meal in laying hens rations and its effects on cecal volatile fatty acids profile and intestinal microbial colonization

This experiment was performed to evaluate the effects of replacing different levels of soybean meal with sunflower meal (with and without enzyme) on cecal volatile fatty acid profile and intestinal microbial colonization in laying hens. In this experiment, 360 laying hens from the age of 47 to 57 weeks, in 9 treatments and five replications (8 hens in each replicate) were performed as a 3 × 3 factorial experiment in a completely randomized design for ten weeks. The results showed that the replacement of 30 and 60 percent sunflower meal and enzyme (100 and 200 gr/ton) has significantly improved the concentration of n-valeric acid on the cecum of laying hens (P&lt; 0.05). Simultaneously, increasing 30 percentage of sunflower meal or 100 of an enzyme enhanced isovaleric acid, n-butyric acid, isobutyric acid, propionic acid, and acetic acid (P&lt; 0.05). Reduction in intestinal log Ecoli was discovered for samples treated with 30 and 60 percent of sunflower (P&lt;0.05), but for log baglus that treated with 30 and 60 percent of sunflower showed higher (P&lt;0.05). The main effects of the enzyme had no significant impact on the cecal microbial population (P&gt;.0.05) while, the effect of meal source and enzyme additive interactions on the tested parameters was significant (P&lt;.0.05). Hence, sunflower meal could be used as an alternative protein source in laying hens ration to improve cecal VFA profile and reduce cecal Ecoli population. Substitution of sunflower meal in laying hens is recommended.

Can Taurine Supplementation in a Diet with Soybean Meal Instead of Fish Meal Improve the Growth Performance, Feed Utilization, and Antioxidant Capacity of Spotted Knifejaw (Oplegnathus punctatus)?

To determine the impact of replacing fish meal (FM) in the diet with various levels of soybean meal (SBM) on the spotted knifejaw Oplegnathus punctatus, a 56 day feeding trial was done. Seven diets were formulated with SBM to replace 0% (SBM0), 30% (SBM30), 40% (SBM40), 50% (SBM50), 60% (SBM60), and 70% (SBM70) of FM protein, and SBM50 + T was developed on the basis of SBM50 with the addition of 1.2% taurine. There were triplicate groups of 18 fish (initial weight: 14.62 ± 0.02 g). The weight gain (WG), specific growth rate (SGR), feed efficiency (FE), and protein efficiency ratio (PER) values of the SBM0, SBM30, and SBM50 + T groups were found to be significantly higher than those of the SBM60 and SBM70 groups (p &lt; 0.05). The daily energy gain (DEG), daily nitrogen gain (DNG), daily lipid gain (DLG), energy retention (ER), nitrogen retention (NR), and lipid retention (LR) values decreased significantly with increasing dietary SBM levels (p &lt; 0.05). The highest retention of most amino acids (except lysine) was observed in the SBM30 group (p &lt; 0.05). The lipid content of the whole body and dorsal muscle decreased significantly as dietary SBM levels increased (p &lt; 0.05). Fish fed the SBM70 diet had the lowest serum triglyceride (TG) concentrations (p &lt; 0.05). The effects of different treatments on total cholesterol (T-CHO) were not significant (p &gt; 0.05). Fish fed the SBM0 and SBM30 diets had the highest amylase (AMS) and lipase (LPS) activities (p &lt; 0.05). The lowest liver superoxide dismutase (SOD) and catalase (CAT) activities were observed in the SBM70 group. The malondialdehyde (MDA) concentration of the SBM50 to SBM70 groups were significantly higher than that of other groups (p &lt; 0.05). The levels of interleukin 8 (il-8) mRNA were highest in fish fed the SBM0, SBM30, and SBM50 + T diets (p &lt; 0.05), while the level of transforming growth factor β1 (tgf-β1) was the opposite (p &lt; 0.05). According to the broken line regression of WG and FE, the highest level of FM substitution by SBM for Oplegnathus punctatus was 24.07–25.31%.