Soybean Litter Research Articles

Aktivitas Nitrat Reduktase (ANR) Tanaman Jagung pada Pola Tumpangsari yang Diberi Serasah Jagung-Kedelai serta Biochar di Lahan Suboptimal Sidondo Sulawesi Tengah

The aim of the study was to examine the relationship between nitrate reductase activity and N uptake, tissue N content, N uptake, and chlorophyll content of corn plants in intercropping patterns which are given corn-soybean litter and biochar. The use of plant litter and biochar derived from crop residues as a source of organic matter in different intercropping patterns affected nitrate reductase activity, tissue N content, N uptake, and chlorophyll content of maize. This experiment was carried out on sub-optimal Sidondo land, Central Sulawesi with the main limiting factors being the availability of water and alkaline soil pH (7.21) which were arranged in a divided plot design with five litter treatments as subplots and five cropping patterns as the main plot with three replicates. Parameters observed were N levels, plant tissue N uptake, chlorophyll, and nitrate reductase activity. Collected data were analyzed using Anova and Duncan's multiple random tests. The results showed that corn litter, soybean mixed corn + soybean litter and their biochar in different intercropping patterns affected nitrate reductase activity, nitrogen uptake, chlorophyll levels very significantly (P˂0.01), increased nitrate reductase activity by 9710.86 mol NO2- -1g-1hour-1 at the age of 55 DAP, while N uptake (2237 mg.kg-1), tissue N content (4.33%), and total chlorophyll content (10.61 mg.l-1) were the highest in the litter treatment, corn and soybeans as well as biochar in corn and soybean intercropping (1:2), and (2:4). Thus, the provision of corn and soybean litter and biochar in intercropping systems can increase nitrate reductase activity, N uptake, tissue N levels, and corn chlorophyll levels in the corn-soybean intercropping system.
 
 Keywords: chlorophyll, N content of corn plant tissue, nitrate reductase activity, N uptake

PENGARUH JENIS DAN CARA APLIKASI BAHAN ORGANIK TERHADAP HASIL PADI (ORYZA SATIVA L.) DI MEDIA TANAM GAMBUT

This study aims to determine the types and ways of application of organic matter and the combination of both of the rice yields in peat growing media. This research has been carried out in the Experimental Garden of the Faculty of Agric ulture, Riau University, Campus Bina Widya Km 12.5, Simpang Baru Panam Sub-District, Tampan District, Pekanbaru, Riau. This research was carried out for 4 months, starting from May to August 2018. The research was conducted experimentally arranged according to a completely randomized design (CRD) factorial pattern consisting of 2 factors. Factor I: organic matter of plant waste (O), namely: O1 (125 g rice straw), O2 (125 g soybean litter), O3 (oil palm empty fruit bunches 125 g) and Fact II: how to apply organic material (C), namely: C1 (mixed) and C2 (spread). The results obtained were analyzed using variance analysis and continued with the double range duncan's new multiple range test (DNMRT) at 5% level. However, the type of organic rice paddy yields results in the weight of the milled dry grain which is 106.94 g-138.23 g, the weight of 1,000 grains that is 26.40 g-26.95 g and soybean litter on the percentage of rice grain grain 86.24 g-88.00 g. Giving application method in the distribution gives results on the weight of dry milled unhulled rice that is 125.21 g-126.02 g, the weight of 1,000 grains that is 26.65 g-26.79 g and the percentage of unhulled grain that is 86.76 g-87 , 74 g and the combination of the type of organic rice paddy and the application method in the distribution gives the results of the weight of the milled dry rice which is 102.94 g - 140.87 g, the weight of 1,000 items, which is between 26.29 g - 27.27 g and soybean litter organic material types and application method are mixed namely 84.94 g - 88.50 g. Key words : application method, rice plant, rice straw, soybean litter, TKKS .

Compatibility of biofresh and agrisoy biological agents in various types of organic materials against increased resistance to mosaic virus and soy productivity in ultisol land

The subject of the research was aimed to assess the compatibility of organic matter and bio-fertilizers type in improving plant resistance to disease mosaic virus (SMV) and the production of soybean on Ultisol. This study was conducted by two phases; the first phase was compatibility testing of Bokashi types and biological fertilizers to improve plant resistance to disease mosaic virus (Soybean mosaic virus) and Production of Soybean (Glycine max L. Merrill), organized by Split plot Design in a randomized block design consisting of 16 treatment repeated three replications. Therefore, there were 48 experimental units. Data were analyzed using analysis of variance followed by Duncan’s Multiple Range Test if the treatment significantly. The results showed that both of the biological agents Biofresh and Agrisoy that applied with soybean litter compost is able to improve the resistance to disease mosaic virus (Soybean mosaic virus) and productivity of soybean in Ultisol.

Carbon sequestration under different organic amendments in saline-alkaline soils

Organic amendments are a recyclable resource and can improve soil quality and carbon (C) sequestration, however, the difference in their effects on soil aggregate formation and C sequestration in saline-alkaline soils are less studied. We examined the effects of organic amendments to saline rice paddy fields with 4 treatments: (i) basal paddy soil (CK) lacking amendments (ii) organic fertilizer (OF) treatment at 910 kg C ha−1 from soybean litter and beans (iii) rice straw (RS) treatment at 3600 kg C ha−1 and (iv) rice straw-derived biochar (RS-biochar) treatment at 3600 kg C ha−1. All organic amendments significantly increased the soil organic C (SOC) stock in the 0–30 cm soil layer compared to CK. The SOC stock in the biochar treatment increased by 6.2 and 40.4% over the OF and RS amended treatments, respectively. This was most likely due to the greater levels of aromatic C in RS-biochar at 1.22 and 1.24 times greater than for the other organic amendments, respectively. Soil aggregates of 2–8 mm at 0–15 cm in the OF and RS amended soils were 38.3 and 58.2%, respectively, higher than that from the RS-biochar treatment. The mean weight diameter (MWD) in the OF and RS treatments were 35.4% and 45.8% higher than for the RS-biochar, respectively. MWD was significantly negatively correlated with soil exchangeable Na (p < 0.05). This indicated OF and RS were better than RS-biochar in improving soil aggregate stability. In addition, the C management index values for RS were 21.6 and 45.8% greater than for OF and RS-biochar in the 0–15 cm soil layer, respectively. These data indicated that saline soil aggregate formation and C sequestration improved after organic amendments application, which caused the exchangeable Na to decrease and affected its distribution in aggregates, and changed the composition of C functional groups.

Do corn-soybean rotations enhance decomposition of soil organic matter?

Corn and soybean crops are often grown in rotation, requiring lower nitrogen (N) inputs than continuous corn. However, soil organic carbon (C) may be declining in corn-soybean systems despite sustained residue inputs. We asked whether corn-soybean rotations increase decomposition of litter and soil C as compared with continuous corn. We incubated soils from a long-term field experiment including continuous corn and both phases of the corn-soybean rotation. Soils were amended with corn litter, soybean litter, or no litter. We measured natural abundance C stable isotopes (δ13C values) in respiration and microbial biomass to partition C sources. Addition of soybean litter increased microbial biomass while corn did not. However, corn litter addition consistently increased (i.e., primed) soil C decomposition while soybean litter did not. Soils most recently planted to corn following soybeans had the greatest soil C decomposition and N mineralization irrespective of litter addition, and they decomposed corn litter faster and had a faster priming response than the other treatments. Our data support the hypothesis that alternating inputs of N-rich soybean litter and relatively N-poor corn litter could enhance litter and SOC decomposition by driving microbial growth following the soybean phase and stimulating priming following the corn phase. Increased decomposition and N mineralization from litter and SOC in corn-soybean rotations may contribute to the soybean N credit but could also contribute to longer-term soil C and N declines, consistent with field data.

Microdialysis as an in situ technique for sampling soil enzymes

Soil extracellular enzyme activity (EEA) represents a critical bottleneck in the release of bioavailable nutrients from organic materials. However, quantifying spatial and temporal dynamics of EEA remains challenging. Techniques which measure the activity of, or directly sample free enzymes in situ may assist in understanding the short-term exoproteomic responses of microbes and roots to substrates, but few tools exist to explore EEA with minimal disturbance. We explore the potential of in situ microdialysis to directly sample soil enzymes, measuring their activity using a modified enzyme assay. We hypothesise that the technique's bias towards free solutes will also allow differentiation of free and stabilised enzyme pools. As little is known about the efficiency of microdialysis to sample enzymes from soil, recovery of a protease standard was quantified from solution and soil, finding that enzyme recovery is hindered at lower soil moisture contents. We further measured the response of native protease activity after the addition of soybean litter to clay and sandy soils, finding microdialysis observed greater EEA in litter-amended treatments than controls in both soil types. In comparison, EEA as measured by conventional extraction-incubation methods was only greater in amended clay soils. In a final experiment, hydrolytic enzyme activity of free and stabilised clay soil fractions were estimated using microdialysis. Free enzymes contributed 9% of total hydrolytic activity in soil without litter, increasing to 46% in litter-amended soil, suggesting fresh litter promoted a transient increase in the production of free exoenzymes by soil microbes. In contrast, the addition of litter had no significant effect on stabilised EEA. In spite of the obvious challenges involved in applying microdialysis as a method for soil protein sampling, this method offers new possibilities for investigating challenging spatial and temporal aspects of enzyme dynamics and protein availability in soils.

Produksi dan Kualitas Benih Kedelai dalam Sistem Produksi Bersih

Production of high quality seed is one of the key factors for soybean self-sufficiency. The study was aimed to produce high quality soybean seed from zero waste system. The trial was carried out at Sebapo Experimental Station, Jambi, Center for Agricultural Post Harvest Research and Development, and Center for Agricultural Land Resources Research and Development, The Ministry of Agriculture, Bogor, from January until October 2017. The experiment used a complete randomized design with a treatments combination both organic nutrient of composted soybean litter (5 tons ha-1) and inorganic nutrient (25 kg Nitrogen ha-1, 50 kg P2O5 ha-1, and 50 kg K2O ha-1). The treatments were designed as follow : K0 (control), K1 (100% organic), K2 (75% organic + 25% inorganic), K3 (50% organic + 50% inorganic), K4 (25% organic + 75% inorganic), and K5 (100% inorganic). The results showed that the treatment K3 produced the highest seed production (1.72 tons ha-1) and the highest 1,000 seed weight (147.71 g). After 3 months storage at room temperature, the seed with treatment K3 could maintain its quality with indicator 1,000 seed weight (140.98 g), 10.82% water content, 34.98% protein content, 57.42 μScm-1g-1 electrical conductivity, 80.98% germination percentage and 27.48% etmal-1 rate of germination speed.Keywords: Compost, litter, seed technology

Induction of soybean resistance to bacterial pustule disease (Xanthomonas axonopodis pv. glycines) by rhizobacteria and organic material treatment

This study aimed to evaluate the role of different formulations and types of organic matter in improving yield and resistance of soybean plants to bacterial pustule disease. The study was prepared based on a randomized block design with a factorial pattern. The first factor was the application of rhizobacterial formulation (biofresh), ie F0 = without the application of rhizobacteria, F1 = application of biofresh in solid formulation, and F2 = application of biofresh in liquid formulation. The second factor was the application of organic materials, namely B1 = compost of soybean litter + cow dung, B2 = compost of rice straw + cow dung, B3 = compost of soybean litter + rice straw + cow dung. Observation of disease severity and soybean yield was conducted on five sample plants in each treatment. The results showed that the treatment of biological agent biofresh in solid formulation combined with compos of soybean litter, was the best treatment in increasing plant resistance to bacterial pustule disease and seed weight. Plant resistance induction occurred systemically characterized by salicylic acid increase of 0.3 mg and peroxidase increase of 0.07 unit / mL in the sample plants.

Microbial function in adjacent subtropical forest and agricultural soil

Soil microbial communities and their activities are altered by land use change; however impacts and extent of these alterations are often unclear. We investigated the functional responses of soil microbes in agricultural soil under sugarcane and corresponding native soil under Eucalyptus forest to additions of contrasting plant litter derived from soybean, sugarcane and Eucalyptus in a microcosm system, using a suite of complimentary techniques including enzyme assays and community level physiological profiles (CLPP). Initially agricultural soil had 50% less microbial biomass and lower enzyme activities than forest soil, but significantly higher nitrification rates. In response to litter addition, microbial biomass increased up to 11-fold in agricultural soil, but only 1.8-fold in forest soil, suggesting a prevalence of rapidly proliferating ‘r’ and slower growing ‘K’ strategists in the respective soils. Litter-driven change in microbial biomass and activities were short lived, largely returning to pre-litter addition levels by day 150. Decomposition rates of sugarcane and soybean litter as estimated via CO2 production were lower in agricultural than in forest soil, but decomposition of more recalcitrant Eucalyptus litter was similar in both soils, contradicting the notion that microbial communities specialise in decomposing litter of the dominant local plant species. Enzyme activities and community level physiological profiles (CLPP) were closely correlated to microbial biomass and overall CO2 production in the agricultural soil but not the forest soil, suggesting contrasting relationships between microbial population dynamics and activity in the two soils. Activities of enzymes that break down complex biopolymers, such as protease, cellulase and phenol oxidase were similar or higher in the agricultural soil, which suggests that the production of extracellular biopolymer-degrading enzymes was not a factor limiting litter decomposition. Enzyme and CLPP analyses produced contrasting profiles of microbial activity in the two soils; however the combination of both analyses offers additional insights into the changes in microbial function and community dynamics that occur after conversion of forest to agricultural land.

Assessing the effect of organic residue quality on active decomposing fungi in a tropical Vertisol using 15N-DNA stable isotope probing

15N-DNA stable isotope probing (15N-DNA-SIP) combined with 18S rRNA gene-based community analysis was used to identify active fungi involved in decomposition of 15N-labeled maize and soybean litter in a tropical Vertisol. Phylogenetic analysis of 15N-labeled DNA subjected to 18S rRNA gene-based community fingerprinting showed that organic residue quality promoted either slow (i.e. Penicillium sp., Aspergillus sp.) or fast growing (i.e. Fusarium sp., Mortierella sp.) fungal decomposers in soils treated with maize or soybean residues, respectively, whereas Chaetomium sp. were found as dominant decomposers in both residue treatments. Therefore, we have clear evidence that specific members of the fungal community used 15N derived from the two different organic resources for growth and stimulated early decomposition of maize or soybean decomposition. In conclusion, our study showed that 15N-DNA-SIP-based community analyses cannot only follow the flow of N from organic resources into bacteria, but also into the actively decomposing fungal communities of soils.

Bioethanol production from dedicated energy crops and residues in Arkansas, USA

Globally, one of the major technologic goals is to achieve cost-effective lignocellulosic ethanol production from biomass feedstocks. Lignocellulosic biomass of four dedicated energy crops [giant reed (Arundo donax L.), elephantgrass (Pennisetum purpureum (Schumach), Miscanthus × giganteus (Illinois clone), and (clone Q42641) {hybrid of Miscanthus sinensis Anderss. and Miscanthus sacchariflorus (Maxim)}, Hack. called giant miscanthus, and sugarcane clone US 84-1028 (Saccharum L. spp. hybrid)] and residues from two crops [soybean (Glycine max (L.) Merr.) litter and rice (Oryza sativa L.) husk] were tested for bioethanol production using cellulose solvent-based lignocellulose fractionation (CSLF) pretreatment and enzymatic (cellulase) hydrolysis. Giant miscanthus (Illinois), giant reed, giant miscanthus (Q42641), elephantgrass, and sugarcane all yielded higher amount of glucose on a biomass dry weight basis (0.290-0.331 g/g), than did rice husk (0.181 g/g) and soybean litter (0.186 g/g). To reduce the capital investment for energy consumption in fermentation, we used a self-flocculating yeast strain (SPSC01) to ferment the lignocellulosic biomass hydrolysates. Bioethanol production was ∼0.1 g/g in dedicated energy crops and less in two crop residues. These methods and data can help to develop a cost-effective downstream process for bioethanol production.

Litter quality effects on soil carbon and nitrogen dynamics in temperate alley cropping systems

Microcosm and litterbag experiments were conducted to determine the effects of litter quality, soil properties and microclimate differences on soil carbon (C) and nitrogen (N) mineralization in alley cropping systems. Bulk soils were collected from 0 to 20 cm depth at three sites: a 21-year old pecan ( Carya illinoinensis)/bluegrass ( Poa trivialis) intercrop (Pecan site) in north-central Missouri, a 12-year old silver maple ( Acer saccharinum)/soybean ( Glycine max)–maize ( Zea mays) rotation (Maple site) in northeastern Missouri and a restored prairie site (MDC site) in southwestern Missouri. Seven tree and crop litters with varying composition were collected, including pecan, silver maple, chestnut and walnut leaf litter (tree litter) and maize, soybean and bluegrass residues (crop litter). Aerobic microcosm incubations were maintained at 25 °C and a soil water potential of −47 kPa. Unamended MDC soil mineralized 24 and 18% more CO 2 than the Pecan and Maple soils, respectively. Soil amended with crop litter mineralized on average 32% more CO 2 than when amended with tree litter. Net N mineralization from soybean litter was 40 mg kg −1, while all other litter immobilized N for various durations. A double pool and a single pool model best described C and N mineralization from amended soils, respectively. Cumulative CO 2 mineralized, labile C fraction ( C 1) and potentially mineralizable C ( C 0) were correlated to litter total N and lignin contents and to (lignin + polyphenol):N ratio. In the field, bluegrass litter decomposed and released N twice as fast as pecan leaf litter. Soybean, maize and silver maple litter released 84, 75 and 63% of initial N, respectively, 308 days after field placement, while no differences in mass loss was observed among the three litter materials. At the Maple site, mass and N remaining, 308 days after field placement was lower at the middle of the alley, corresponding to higher soil temperature and water content. No differences in mass loss and N release patterns were observed at the Pecan site. Microclimate and litter quality effects can lead to differences in nutrient availability in alley cropping systems.

Spectral detection of crop residues for soil conservation from conventional and large biomass soybean

A spectrally derived cellulose absorption index (CAI) was tested to determine its value as a remote sensing method for detecting crop residue ground coverage for soil erosion control in soybean (Glycine max (L.) Merr.). Soybean produces inadequate crop residue for soil conservation purposes in many production years. Crop residues left on the soil surface after harvest slow soil erosion rates. The CAI remote sensing technique was tested over field plots of conventional and large biomass soybean (LBS) with known above ground crop residue biomass and surface coverage. New LBS types are being bred and tested at the Beltsville Agricultural Research Center (BARC), Beltsville, Maryland, US, and can grow to heights of 1.8 m and produce increased amounts of crop residue compared to conventional cultivars. The highest performing LBS line for these traits provided 2963 kg/ha more crop residue biomass and provided a maximum increase of 42% more crop residue cover than the poorest performing conventional soybean. The comparison of LBS versus conventional soybean provided a wide range of soybean residue coverage for testing the CAI remote sensing algorithm. Spectrally derived CAI measures of crop residue were significantly associated with physical ground measurements of crop biomass at harvest and % cover after over wintering. Significant correlations were found between, the CAI and at harvest biomass (r 2 = 0.66), between, the CAI and the line point transect measurement (r 2 = 0.74), and between, CAI and the analysis of red-green-blue digital imagery (r 2 = 0.74) for measuring crop residue cover. These findings indicate that LBS can increase crop residue biomass and crop residue soil coverage by soybean litter and these factors can be detected by remote sensing methods in the field.