Soybean By-products Research Articles

Valorization of soybean by-products for sustainable waste processing with health benefits.

Soybean is a rapidly growing agricultural crop, fueled by the rising global demand for animal feed, plant-based proteins and essential nutrients for human consumption. Soybeans contain a wide range of essential nutrients that are vital for health and may play a significant role in disease prevention. Their nutritious composition has led to a diverse range of soy-based foods and derivatives available on the market. A substantial amount of soybeans is allocated to the animal feed sector, human consumption, nutraceuticals and other industrial applications. Soybean has superior protein quality and digestibility compared to other legumes. It also contains abundant amounts of isoflavones, phytosterols and minerals that augment its nutritional value as a constituent of the human diet. Many different by-products are produced during the processing of soy. Due to a lack of sustainable food manufacturing practices, a sustainable amount of these by-products is discarded as waste, posing environmental challenges. Developing an effective waste management system for soybean by-products can help address public health concerns and provide a cost-efficient way to repurpose valuable components of soy, such as soy meal, okara and soy whey. Such valorization can mitigate the environmental impact of waste and contribute to malnutrition. This study aims to evaluate the sustainable use of soy by-products to help preserve biodiversity and reduce food insecurity. This article thoroughly examines the fundamental components of soybean use in the fields of health and soybean by-product processing. It provides a summary of cost-efficient, feasible and ideal processing technologies. © 2024 Society of Chemical Industry.

Comprehensive analysis of soybean (Glycine max L.) by-products: nutritional value, phenolic composition, and bioactive properties

Soybeans are key components in vegetable beverage production, generating two by-products: soybean hulls and okara. For every ton of soybeans, 50-80 kg of hulls and 120 kg of okara are produced, being often discarded or used in low-value applications like fertilizers or feed. This study aims at characterizing their biochemical and nutritional profiles to assess their potential reintroduction into the food chain. Both by-products have high levels of protein and dietary fiber, mainly insoluble. Okara and hulls predominantly contain oleic acid and linoleic acid, respectively. Seventeen phenolic compounds, mainly isoflavones, were identified, with genistein as the main compound. Hulls exhibit superior antioxidant activity compared to okara. Neither extract showed cytotoxicity or anti-inflammatory effects and exhibited limited antimicrobial activity. However, both demonstrate prebiotic potential, promoting beneficial gut bacteria growth. The results suggest that these by-products have significant potential as new ingredients for their protein, isoflavone, and fiber content, alongside prebiotic properties.

Synthesis of gold nanoparticles using soybean byproducts: applications in catalysis

AbstractThis study demonstrates the feasibility of extracting lecithin from oil industry byproducts in an eco‐friendly manner, with minimal use of water and without harmful chemicals. Liposomes can be generated directly from this extracted lecithin, enhancing the value of these byproducts and enabling the production of catalytic gold nanoparticles (AuNPs). Thin‐layer chromatography of the extracted lecithin revealed a phospholipid composition primarily consisting of phosphatidylethanolamine and phosphatidylcholine, and surface tension studies demonstrated similar behavior between the extracted and commercial lecithin. Liposome formation using sustainable lecithin (LPn) resulted in structures that were stable for at least 10 days, exhibiting a low polydispersity index (0.395) and uniform size (approximately 214 ± 7 nm). Gold nanoparticles were synthesized successfully in LPn loaded with [HAuCl4] by using different photoreduction methods: ultraviolet (UV) lamp, pulsed laser 355 nm, and sunlight irradiation. The AuNPs exhibited characteristic sizes (ranging from 5.03 to 6.78 nm) and optical properties typical of nanoparticles, including a distinct surface plasmon resonance. As a proof of concept, we also demonstrated that the synthesized AuNPs exhibited catalytic activity in UV‐induced cis‐trans isomerization reactions. Overall, the study highlights the potential of sustainable soy lecithin extraction for diverse applications, including nanoparticle synthesis and catalysis.

Ultrasound-assisted process to improve proteins recovery from industrial canola and soybean byproducts

AbstractAn ultrasound-assisted process is proposed to obtain protein isolates from canola and soybean byproducts. A laboratory-scale probe system (24 kHz, 400W) was used for protein extraction and six levels of energy density were analyzed. Different mixtures of solvents (water, ethanol, methanol, acetone) and temperatures were tested for removing phenolic compounds, and the best results were obtained with ethanol-water (8:2 v/v) and energy density of 104 kJ/L, which minimize protein losses at that step. After extraction of phenolic compounds, samples were alkalinized (pH 12 or 9.5), subjected to ultrasound treatment and the protein precipitated at the isoelectric pH. The highest yield on protein extraction was obtained with 1:20 meal: NaOH solution ratio, applying 150 kJ/L at constant temperature (30 °C), increasing the protein recovery, 72.5% for canola meal and 37.5% for soybean meal, compared to the conventional method. The solubility of both isolates obtained with ultrasound improved ~ 50% at a pH close to neutral and basic, with an increase in free sulfhydryl groups, without significant effects of ultrasound treatment on the subunit fractions of the proteins. These results provide alternatives to develop protein isolates from an undervalued by-product, with better techno-functional properties that can be employed in the food industry.

A new biostimulant derived from soybean by-products enhances plant tolerance to abiotic stress triggered by ozone

BackgroundTropospheric ozone is an air pollutant that causes negative effects on vegetation, leading to significant losses in crop productivity. It is generated by chemical reactions in the presence of sunlight between primary pollutants resulting from human activity, such as nitrogen oxides and volatile organic compounds. Due to the constantly increasing emission of ozone precursors, together with the influence of a warming climate on ozone levels, crop losses may be aggravated in the future. Therefore, the search for solutions to mitigate these losses becomes a priority.Ozone-induced abiotic stress is mainly due to reactive oxygen species generated by the spontaneous decomposition of ozone once it reaches the apoplast. In this regard, compounds with antioxidant activity offer a viable option to alleviate ozone-induced damage. Using enzymatic technology, we have developed a process that enables the production of an extract with biostimulant properties from okara, an industrial soybean byproduct. The biostimulant, named as OEE (Okara Enzymatic Extract), is water-soluble and is enriched in bioactive compounds present in okara, such as isoflavones. Additionally, it contains a significant fraction of protein hydrolysates contributing to its functional effect.Given its antioxidant capacity, we aimed to investigate whether OEE could alleviate ozone-induced damage in plants. For that, pepper plants (Capsicum annuum) exposed to ozone were treated with a foliar application of OEE.ResultsOEE mitigated ozone-induced damage, as evidenced by the net photosynthetic rate, electron transport rate, effective quantum yield of PSII, and delayed fluorescence. This protection was confirmed by the level of expression of genes associated with photosystem II. The beneficial effect was primarily due to its antioxidant activity, as evidenced by the lipid peroxidation rate measured through malondialdehyde content. Additionally, OEE triggered a mild oxidative response, indicated by increased activities of antioxidant enzymes in leaves (catalase, superoxide dismutase, and guaiacol peroxidase) and the oxidative stress index, providing further protection against ozone-induced stress.ConclusionsThe present results support that OEE protects plants from ozone exposure. Taking into consideration that the promotion of plant resistance against abiotic damage is an important goal of biostimulants, we assume that its use as a new biostimulant could be considered.

268 Effect of soybean gums and soybean soapstocks on weanling pig growth performance

Abstract Currently, the soybean processing industry has not fully characterized the composition or nutritional value of certain processing by-products (e.g., gums, phospholipid) and their effect on soybean meal (SBM) quality. Depending on the processing plant, soybean gums and soapstocks may be added back to SBM during manufacturing. There is potential for these by-products to serve as an affordable energy source for swine due to their residual oil content. For this study, pigs [n = 350; Line 241 × 600, DNA; initial body weight (BW) = 5.3 ± 0.02 kg] were weaned at approximately 19 d of age and used in a 42-d experiment. At weaning, pigs were randomly assigned to pens and allotted to 1 of 5 dietary treatments. There were 5 pigs per pen and 14 pens per treatment. Diets were fed in 3 phases: phase 1 from weaning to d 11, phase 2 from d 11 to 23, and phase 3 from d 23 to 42. Treatments included a control diet containing SBM with no added soybean by-products. Two additional diets contained gums or soapstocks at 4% of the SBM level in the diet. Another treatment diet contained SBM with 2% added soybean gums and 2% added soybean soapstocks. Lastly, a negative control contained 4% less SBM with no added by-products to have equal protein from soybeans as the diets with added gums or soapstocks. From d 0 to 11 (phase 1) and d 11 to 23 (phase 2), there was no evidence of differences (P > 0.10) for any growth response criteria (Table). From d 23 to 42 (phase 3), feeding SBM with added gums increased (P = 0.05) average daily gain (ADG). However, there was no evidence for differences (P > 0.10) in average daily feed intake (ADFI) or gain to feed ratio (G:F). For the overall experimental period (d 0 to 42), there was no evidence of differences (P > 0.10) due to dietary treatment in ADG, ADFI, or G:F. In summary, these data suggest that adding soybean processing by-products such as gums or soapstocks had minimal impacts on nursery pig growth performance. However, there is a potential for improved ADG in the late nursery period when SBM containing added gums is included in the diet.

Possibility of immediate treatment to soybean processing by-products using screw extrusion: a mini review

Soybeans are an important oil and protein supply crop, but the utilization of by-products from its processing products has never been effectively resolved, while a large number of nutritional resources have been wasted and environmental pollution is serious, restricting the sustainable development of the industry. The critical problem of soybean processing by-products includes excessive levels of microorganisms, residual proteins, metal ions, and other substances. Because the screw extrusion process has potentially advantageous processing characteristics, it appears that it’s just the right solution to the above problem of soybean processing by-products. We propose that this technology may be a potentially scalable technology for the immediate processing of soybean by-products. This paper reviews the theoretical foundations in existing research, briefly discusses the technological advantages, feasibility, and potential risks of this process, and hopes that researchers may pay attention to this technique and conduct feasibility studies.

Comparison of Emulsion Stabilizers: Application for the Enhancement of the Bioactivity of Lemongrass Essential Oil.

Recent focus on cellulose nanomaterials, particularly biodegradable and biocompatible cellulose nanocrystals (CNCs), has prompted their use as emulsion stabilizers. CNCs, when combined with salt, demonstrate enhanced emulsion stabilization. This study explored three emulsion stabilizers: Tween 80, soybean CNCs with salt (salted CNCs), and a combination of salted CNCs with Tween 80. Soybean CNCs, derived from soybean stover, were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Antifungal testing against Aspergillus flavus revealed increased bioactivity in all lemongrass essential oil (EO)-loaded emulsions compared to pure essential oil. In addition, all three emulsions exhibited a slight reduction in antifungal activity after 30 days of room temperature storage. The release experiment revealed that the EO-loaded nanoemulsion exhibited a slow-release profile. The nanoemulsion stabilized by salted CNCs and Tween 80 exhibited significantly lower release rates when compared to the nanoemulsion stabilized solely by Tween 80, attributed to the gel network formed by salted CNCs. The findings of this study highlight the efficacy of cellulose nanocrystals procured from soybean byproducts in conjunction with synthetic surfactants to create nanoencapsulated essential oils, resulting in improved antimicrobial efficacy and the achievement of sustained release properties.

A review of soybean processing byproducts and their use in swine and poultry diets.

Due to its importance in animal feed, soybean meal has been extensively studied to optimize its use in livestock diets. Despite extensive research, the industry has not fully characterized specific areas of soybean processing such as the inclusion of soybean byproducts added back to soybean meal during processing. Soybean processing byproducts can encompass a large variety of materials including weeds and foreign material, soybean hulls, gums, soapstocks, lecithins, spent bleaching clays, and deodorizer distillates. Despite the potential for being added back to soybean meal when a crushing plant is integrated with an oil refinery, there is currently limited information on the composition of many of these soybean processing byproducts and their subsequent effects on soybean meal quality and animal performance. Therefore, there may be opportunities for a new area of research focused on soybean processing byproducts and their optimal use within the livestock feed industry. This review summarizes the current information on soybean byproducts with a focus on identifying the areas with the greatest potential for future research in swine and poultry nutrition.

Eco-Friendly Silver Nanoparticles Synthesized from a Soybean By-Product with Nematicidal Efficacy against Pratylenchus brachyurus.

This study explores an eco-friendly approach to synthesizing silver nanoparticles (AgNPs) using soybean leaf extracts, employing a reaction with silver nitrate at 65 °C for 2.5 h. Optimal results were achieved at extract concentrations of 3.12 and 6.25 mg of the leaf mL-1, termed 3.12AgNP and 6.25AgNP, respectively. UV-Vis spectrophotometric analysis between 350 and 550 nm exhibited a peak at 410-430 nm, along with a color transition in the suspensions from pale yellow to brown, indicating successful synthesis. Dynamic light scattering (DLS) further delineated the favorable properties of these AgNPs, including nanometric dimensions (73-104 nm), negative charge, and moderate polydispersity, portraying stable and reproducible synthesis reactions. The bioreduction mechanism, possibly expedited by leaf extract constituents such as amino acids, phenolic acids, and polysaccharides, remains to be fully elucidated. Notably, this study underscored the potent nematicidal effectiveness of biosynthesized AgNPs, especially 6.25AgNP, against Pratylenchus brachyurus, which is a common plant-parasitic nematode in tropical soybean cultivation regions. In vitro tests illustrated significant nematicidal activity at concentrations above 25 µmol L-1, while in vivo experiments displayed a pronounced nematode population diminishment in plant roots, particularly with a 6.25AgNP rhizosphere application at concentrations of 500 µmol L-1 or twice at 250 µmol L-1, attaining a reproduction factor below 1 without any morphological nematode alterations. This research highlights the potential of 6.25AgNPs derived from soybean leaf extracts in forging sustainable nematicidal solutions, marking a significant stride toward eco-friendly phytonematode management in soybean cultivation. This novel methodology signals a promising avenue in harnessing botanical resources for nematode control and propelling a greener agricultural horizon.

Green corrosion inhibitor for mild steel extracted from soybean leaves

The use of green-soluble inhibitors in the corrosive medium as alternatives to traditional inhibitors has increased due to the toxicity of the commonly used substances. These novel substances are selected owing to their low cost, ease of application as well as maintenance, and low environmental risk. This work aims to evaluate ethanolic-water extracts from post-harvest soybean (Glycine max) by-products as corrosion inhibitors for mild steel in an aggressive medium of sodium chloride. Soybean extracts were obtained by percolation at a controlled flow rate and temperature. To evaluate the anticorrosive efficiency, polarization curves of AISI 1030 steel were carried out at 1 and 7 days of immersion with different concentrations of the soybean extract. Weight loss measurements were carried out alongside potentiodynamic measurements. The steel samples were analyzed by scanning electron microscopy. The soybean extracts obtained reduced the corrosion rate of the steel, showing an efficiency of 30% at day 1. The inhibition efficiency increased up to 80% after 7 days of incubation with 2000 ppm of the extract. A Langmuir adsorption model was fitted to weight loss measurements. Kads and ΔGads obtained from the model were characteristic of physisorption. The corrosion potential was shifted toward more negative values, classifying the ethanolic-water soybean extract as a cathodic inhibitor. The steel surface for the samples incubated with 2000 ppm of soybean extract was greatly improved showing significantly fewer agglomeration of corrosion products. Soybean leaves are a promising by-product useful to produce ethanolic-water extracts to be used as green corrosion inhibitors.

Inhibitory characteristics of flavonoids from soybean (Glycine max [L.] Merr.) leaf against pancreatic lipase

Several reports on obesity have targeted to determine inhibitors of pancreatic lipase (EC 3.1.1.3, PL), which plays a major role in the digestion of dietary lipids, from natural sources. This study aimed to suggest soybean leaf, a soybean by-product, as a novel source of PL inhibitors and investigate PL inhibitory characteristics of the active constituents. Fluorometric lipase assay revealed that the soybean leaf extract has inhibitory activity against PL. UPLC-MS/MS analysis identified flavonoids (genistein, daidzein, formononetin, taxifolin, diosmetin, and glycitein) as active constituents. Among them, diosmetin and glycitein were novel PL inhibitors with half-maximal inhibitory concentrations of 131.9 ± 8.4 and 127.5 ± 7.4 μM, respectively. The inhibition mode of diosmetin and glycitein were deteremined as competitive type from the enzyme kinetic study, supported by molecular docking simulation. Their bindings to PL were confirmed to follow the static quenching process in fluorescence analysis. Collectively, the results of present study indicates soybean leaf and its flavonoids as a promising natural source for antiobesity functional food.

Productivity of soybean varieties of different maturity groups depending on plant density under drip irrigation in the South of Ukraine

Soybeans are a leading leguminous crop for universal use, therefore, measures aimed at increasing their productivity, including the reasonable selection of varietal resources and optimisation of planting density, will make a significant contribution to the creation of sustainable food production systems. The research aims to determine the effect of plant density on the productivity of soybean varieties of different maturity groups. Field laboratory and statistical (correlation and regression analysis) methods were used in the study. The field experiments were conducted during 2018-2020 on dark chestnut soil of a private agricultural enterprise of the agricultural company “Syvash” in Novotroitsk district of Kherson region. Its maximum values for the cultivation of early ripening varieties were ensured by a plant density of 700 thousand/ha, medium early varieties – 500-700 thousand/ha, and medium ripening varieties – 500 thousand/ha. A strong and very strong correlation between yield and soybean yield index was established. Increasing the length of the growing season contributed to the growth of yields. In the group of early maturing soybean varieties, the Monarch variety was determined to be the most productive. There was no significant difference in the level of yield of varieties within other maturity groups. For each variety, the optimum planting density was determined, at which the maximum grain yield was formed. Longer vegetation contributed to a greater accumulation of protein and fat in the grain. In terms of protein content, the difference between varieties of the same maturity group did not exceed 0.2%, but varieties differed in terms of conditional protein yield per hectare of crops. There was no difference in the fat content of mid-season soybean varieties. Among the early-ripening varieties, the Monarch variety was distinguished by higher oil content, and among the mediumearly varieties – by Aratta. The maximum conditional fat yield per hectare of crops was also determined for these varieties. A very strong and strong correlation was found between soybean grain yield and protein and fat content, respectively. A very strong correlation was also found between the protein and fat content of the grain. To ensure sustainable production and efficient use of soybeans and soybean by-products, it is advisable to take the results of the study into account in production conditions

A Performance Study on 3D-Printed Bioplastic Pots from Soybean By-Products

Sustainability is a key factor in the development of new materials for plant pots, given the significant environmental impact of traditional plastic-based pots. Researchers have paid attention to developing biodegradable and sustainable alternatives to petroleum-based pots. In this study, two novel bioplastic formulations are developed, which incorporated soy-based by-product fractions to produce plant pots with self-fertilizing capability while also being cost-competitive. A 3D-printing process, fused filament fabrication, is used to produce plant containers from the filaments of soy-based new materials. Further, a small-scale greenhouse experiment is conducted to compare the performance of the soy-based 3D-printed bioplastic pots with pure polylactic acid (PLA) 3D-printed pots and traditional plastic pots, by growing a fruit-bearing plant (tomato) and a flowering plant (zinnia). Plant growth properties and root circling are analyzed, and the results show that the soy-based pots performed comparably to traditional plastic pots, especially in dry conditions, and also reduced root circling. While a more in-depth analysis is necessary, these initial findings suggest that using soy-based fractions and 3D-printing technology could provide a sustainable approach to developing plant pots, which could reduce the environmental impact of plastic-based containers and improve plant health.

Soybean By-Products Bioplastic (Polylactic Acid)-Based Plant Containers: Sustainable Development and Performance Study

In most cases, plant containers used in the horticulture industry are not reusable, have many negative impacts on the environment, and take a long time to degrade. To reduce the use of non-biodegradable plant containers, many bio-degradable plant containers have been developed for the horticulture industry. However, the full potential of the sustainability of plant containers is yet to be completely explored. Therefore, two novel biodegradable plant containers are developed in this research to effectively contribute to sustainability’s environmental, social, and economic dimensions. The two biocomposite formulations are developed by mixing soy hull and soy protein isolate (SPI) with polylactic acid (PLA) matrix for plant containers. In the first formulation, the proportion of PLA and soy hulls are 70 wt% and 30 wt%, respectively; in the second formulation, 65 wt% PLA is blended with 30 wt% soy hulls and 5 wt% SPI. The injection molding process is used to manufacture the plant containers from the two formulations. In a field trial, four plant species are grown in the novel plant containers along with polyethylene and commercial PLA/DDGS (dried distiller’s grains with solubles and PLA-based container) containers to investigate biodegradability and plant growth. The results show that the containers from the new formulations outperform existing biodegradable PLA/DDGS containers in terms of certain plant growth and biodegradability.

Урожайність пшениці озимої в умовах дефіциту вологи на дерново-підзолистому ґрунті

Goal. To investigate the peculiarities of the formation of productivity and grain quality of winter wheat at different levels of organo-mineral nutrition in the Polissia zone. Methods. Field — stationary experiment; laboratory — determination of quality indicators of soil and grain according to the generally accepted methods; the processing of experimental data — using Microsoft Office Excel computer program. Results. Research conducted during 2016–2020 on the dynamics of moisture reserves in sod-podzolic drained soil had established that during the period of grain formation and swelling, productive moisture reserves in the 0–100 cm soil layer decreased to a critical level of 60–80 mm (by 130–200 mm — during the period of vegetation recovery). In conditions of low soil moisture, 2.67 t/ha of grain (14.1% increase compared to absolute control) was obtained under the biological fertilizer system (byproducts of soybeans). Against the background of the by-products the introduction of the recommended for the zone dose of mineral fertili­zers (N60P60K60), the yield of grain increased to 3.52 t/ha. The highest grain productivity (3.86 t/ha) was obtained with the use of an increased (intensive) dose of mineral fertilizers (N90P90K90) with a se­parate application of nitrogen in 3 stages (N30 — for sowing, N30 — at the output to the tube, N30 — at grain swelling) in combination with side products. The fullness of the wheat grain did not depend significantly on the fertilizer system. The weight of 1000 seeds was 41.0–43.0 g, the grain quality index was within 738–748 g/l. The protein content in the versions with fertilizer application was 13.7–14.2%, on the increased background — 14.5%, which was 1.6% more than the indicator in the control version. Conclusions. The highest level of profitability of growing winter wheat was achieved under the biological fertilizer system using soybean by-products — 150%. Although the application of the enhanced fertilizer system (N90P90K90) in combination with by-products resulted in the highest grain yield (3.52 t/ha) and its protein content (14.5%), the profitability of winter wheat cultivation decreased to 68%. That is, mineral fertilizers increase and improve the quality indicators of grain and at the same time reduce the profitability of crop cultivation.

Linoleic acid enrichment of cheese by okara flour and Geotrichum candidum overexpressing Δ12 fatty acid desaturase.

Mold-ripened cheeses have low levels of unsaturated fatty acids (UFAs). Geotrichum candidum is an adjunct culture for the development of Geotrichum-ripened cheese but has a low ability to produce high levels of UFAs. Δ12 fatty acid desaturase (FADS12) is a pivotal enzyme that converts oleic acid (OA) to linoleic acid (LA) and plays a vital role in UFA biosynthesis. By investigating FADS12 catalytic activity from various species with OA substrates, we found that FADS12 from Mucor circinelloides (McFADS12) had the highest catalytic activity for OA. In the current study, a plasmid harboring McFADS12 was constructed and overexpressed in G. candidum. Our results showed that LA production increased to 31.1 ± 1.4% in engineered G. candidum - three times higher than that in wild-type G. candidum. To enhance LA production, an exogenous substrate (OA) was supplemented, and the yield of LA was increased to 154 ± 6 mg L-1 in engineered G. candidum. Engineered G. candidum was used as an adjunct culture for Geotrichum-ripened cheese production. The LA level reached 74.3 ± 5.4 g kg-1 cheese, whereas the level of saturated fatty acids (SFAs) decreased by 9.9 ± 0.5%. In addition, the soybean byproduct (okara) was introduced into the engineered G. candidum growth and the level of LA increased to 126 ± 4 g kg-1 cheese and the percentage of UFAs:SFAs increased from 0.8:1 to 1.3:1. This study offers a suitable technology for converting SFAs to UFAs in Geotrichum-ripened cheeses and provides a novel trend for converting soybean waste into a value-added product. © 2022 Society of Chemical Industry.

Characterisation of a High Fibre Flour Prepared from Soy Milk By-Product and Its Potential Use in White Wheat Bread.

The commercial production of soy milk renders a large quantity of wet soybean by-product (SMB), which is typically dumped, incinerated, or partially used as animal fodder. This wet SMB has a high moisture content that is rich in nutritional and biologically active compounds. This study aimed to characterise the composition and properties of a flour milled from SMB dried at 100 °C (SMB100) and assess its possible application as a fibre substitute in white bread. The results showed that SMB100 has high levels of dietary fibre (40.6%) and protein (26.5%). It also contains high levels of saponins (31.4 mg/g) and isoflavones (698.0 µg/g). SMB100 has a light-yellow colour with low moisture content and water activity (8.2% and 0.55, respectively). The results also indicated that replacement of wheat flour with SMB100 at 10 or 12.5% by flour weight negatively impacted the raising volume, density, and texture of white bread. Alternatively, substituting wheat flour with 5% of SMB100, did not significantly impact the physical properties of white bread, while significantly improving its dietary fibre content in comparison with the control, revealing that SMB100 is a potential substitute of wheat flour for improvement of dietary fibre in bread. Future studies are needed to optimise bread formulation and improve the processing condition which produces quality white bread with high dietary fibre using SMB100.

Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM

Nattokinase, a serine protease, was discovered in Bacillus subtilis during the fermentation of a soybean byproduct. Nattokinase is essential for the lysis of blood clots and the treatment of cardiac diseases including atherosclerosis, thrombosis, high blood pressure, and stroke. The demand for thrombolytic drugs rises as the prevalence of cardiovascular disease rises, and nattokinase is particularly effective for the treatment of cardiovascular diseases due to its long duration of action. In this study, we cloned the nattokinase gene from the Bacillus subtilis strain into the pET32a vector and expressed the protein in the E. coli BL21(DE3) strain. The active recombinant nattokinase was purified using Ni-NTA affinity chromatography and then evaluated for fibrinolytic and blood clot lysis activity. Physiological parameters for optimizing protein production at optimal pH, temperature, IPTG concentration, and incubation time were investigated. A statistical technique was used to optimize media components for nattokinase overproduction, and Central Composite Design-Response Surface Methodology-based optimization was used to select significant components for protein production. The optimized media produced 1805.50 mg/L of expressed nattokinase and 42.80 gm/L of bacterial mass. The fibrinolytic activity obtained from refolded native protein was 58FU/mg, which was five times higher than the available orokinase drug (11FU/mg). The efficiency with which a statistical technique for media optimization was implemented improved recombinant nattokinase production and provides new information for scale - up nattokinase toward industrial applications.

In Silico Approach of Glycinin and Conglycinin Chains of Soybean By-Product (Okara) Using Papain and Bromelain.

This study explores utilization of a sustainable soybean by-product (okara) based on in silico approach. In silico approaches, as well as the BIOPEP database, PeptideRanker database, Peptide Calculator database (Pepcalc), ToxinPred database, and AllerTop database, were employed to evaluate the potential of glycinin and conglycinin derived peptides as a potential source of bioactive peptides. These major protein precursors have been found as protein in okara as a soybean by-product. Furthermore, primary structure, biological potential, and physicochemical, sensory, and allergenic characteristics of the theoretically released antioxidant peptides were predicted in this research. Glycinin and α subunits of β-conglycinin were selected as potential precursors of bioactive peptides based on in silico analysis. The most notable among these are antioxidant peptides. First, the potential of protein precursors for releasing bioactive peptides was evaluated by determining the frequency of occurrence of fragments with a given activity. Through the BIOPEP database analysis, there are several antioxidant bioactive peptides in glycinin and β and α subunits of β-conglycinin sequences. Then, an in silico proteolysis using selected enzymes (papain, bromelain) to obtain antioxidant peptides was investigated and then analyzed using PeptideRanker and Pepcalc. Allergenic analysis using the AllerTop revealed that all in silico proteolysis-derived antioxidant peptides are probably nonallergenic peptides. We also performed molecular docking against MPO (myeloperoxidases) for this peptide. Overall, the present study highlights that glycinin and β and α subunits of β-conglycinin could be promising precursors of bioactive peptides that have an antioxidant peptide for developing several applications.