Soybean Research Articles

In vivo Evaluation of Saccharomyces-Modified Tempeh as Potential Prebiotic and Probiotic Food using Mus musculus as an Animal Model

Saccharomyces-modified tempeh (SM tempeh), which is produced by adding Saccharomyces cerevisiae during soybean fermentation, is considered to have the potential as a source of prebiotics. The research aims to determine the prebiotic activity score (PAS) of SM tempeh extract against the probiotics S. cerevisiae and Lactobacillus casei, as well as to evaluate the resistance of S. cerevisiae and Escherichia coli in the intestines of mice fed tempeh. The PAS evaluation was carried out using a factorial complete randomized block design with three replications and one-way ANOVA for data analysis followed by the least significant difference test (5%). Meanwhile, microbial survivability was carried out in vivo using male Mus musculus strain mice fed standard feed, and standard feed with tempeh extract supplementation. The results showed that the supplemetation of either SM or commercial tempeh extract to the growth media significantly affected on the microbial load of S. cerevisiae, L. casei and E. coli, but the concentrations of tempeh extract had no significant effect. Apart from that, the concentrations of tempeh extract had no effect on the PAS of S. cerevisiae and L. casei, meaning that it was able to promote the growth of probiotics in the amount added to the media in the range of 2–10%. In addition, the feeding type had a significant effect on the survival of S. cerevisiae and E. coli in the intestines. S. cerevisiae carried on SM tempeh was detected surviving in the mice intestine at a rate of 6.12 log CFU/g, indicating that the tempeh was a probiotic food. Most likely SM tempeh is a synbiotic food.

Genome-Wide Association Study on Imputed Genotypes of 180 Eurasian Soybean Glycine max Varieties for Oil and Protein Contents in Seeds

Soybean (Glycine max) is a leguminous plant with a broad range of applications, particularly in agriculture and food production, where its seed composition—especially oil and protein content—is highly valued. Improving these traits is a primary focus of soybean breeding programs. In this study, we conducted a genome-wide association study (GWAS) to identify genetic loci linked to oil and protein content in seeds, using imputed genotype data for 180 Eurasian soybean varieties and the novel “genotypic twins” approach. This dataset encompassed 87 Russian and European cultivars and 93 breeding lines from Western Siberia. We identified 11 novel loci significantly associated with oil and protein content in seeds (p-value < 1.5 × 10−6), including one locus on chromosome 11 linked to protein content and 10 loci associated with oil content (chromosomes 1, 5, 11, 16, 17, and 18). The protein-associated locus is located near a gene encoding a CBL-interacting protein kinase, which is involved in key biological processes, including stress response mechanisms such as drought and osmotic stress. The oil-associated loci were linked to genes with diverse functions, including lipid transport, nutrient reservoir activity, and stress responses, such as Sec14p-like phosphatidylinositol transfer proteins and Germin-like proteins. These findings suggest that the loci identified not only influence oil and protein content but may also contribute to plant resilience under environmental stress conditions. The data obtained from this study provide valuable genetic markers that can be used in breeding programs to optimize oil and protein content, particularly in varieties adapted to Russian climates, and contribute to the development of high-yielding, nutritionally enhanced soybean cultivars.

The transcription factor GmFULc regulates soybean plant height by binding the promoter of a gibberellin-responsive gene.

Plant height is a crucial agronomic characteristic that substantially influences soybean [Glycine max (L.) Merr.] yield. FRUITFULLc (GmFULc) is a MADS-box transcription factor that acts as a growth promoter in soybean; however, the mechanism by which GmFULc regulates soybean height is unknown. This study revealed that GmFULc:GmFULc (the expression of the GmFULc gene driven by its native promoter) soybeans exhibit increased plant height and longer internodes. Conversely, soybean plants containing fulc mutations showed reduced plant height and shortened internodes. Chromatin immunoprecipitation-qPCR revealed GmFULc promotes the expression of gibberellic acid-stimulated Arabidopsis 14 (GmGASA14) and GmGASA32 by directly binding to G-boxes in their promoter regions, leading to notably increased expression of GmGASA14 and GmGASA32 in GmFULc:GmFULc soybean plants and reduced expression in soybean plants containing the fulc-2 mutation. The GmFULc-mediated enhanced expression of GmGASA14 and GmGASA32 increased the gibberellin signal, which may have inhibited gibberellin synthesis by increasing gibberellin 2-oxidase (GmGA2ox) expression and decreasing GA20ox expression. Our findings suggest that GmFULc promoted the expression of GmGASA genes by directly binding to G-boxes in their promoters to regulate soybean plant height.

Fermented foods affect the seasonal stability of gut bacteria in an Indian rural population

The effect of fermented foods on healthy human gut microbiota structure and function, particularly its seasonal preference and frequent long-term consumption, has been largely uncharacterised. Here, we assess the gut microbiota and metabolite composition of 78 healthy Indian agrarian individuals who differ in the intake of fermented milk and soybean products by seasonal sampling during hot-humid summer, autumn and dry winter. Here we show that, seasonal shifts between the Prevotella- and Bifidobacterium/Ruminococcus-driven community types, or ecological states, and associated fatty acid derivatives, with a bimodal change in Bacteroidota community structure during summer, particularly in fermented milk consumers. Our results associate long-term fermented food consumption with reduced gut microbiota diversity and bacterial load. We identify taxonomic groups that drive the seasonal fluctuation and associated shifts between the two ecological states in gut microbiota. This understanding may pave the way towards developing strategies to sustain a healthy and resilient gut microbiota through dietary interventions.

Genome-wide identification of the wall-associated kinase gene family and their expression patterns under various abiotic stresses in soybean (Glycine max (L.) Merr)

Genome-wide identification of the wall-associated kinase gene family and their expression patterns under various abiotic stresses in soybean (Glycine max (L.) Merr)

GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max)

GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max)

Synergistic fermentation of vitamin B2 (riboflavin) bio-enriched soy milk: optimization and techno-functional characterization of next generation functional vegan foods

Vitamin B2 (riboflavin) is essential for cellular growth, energy production, and redox potential. Certain lactic acid bacteria (LAB) can synthesize B2 in low levels in fermented products, however it is mostly retained inside the cell. This study aimed to develop B2-enriched soymilk by fermenting with B2-producing probiotic Lactiplantibacillus plantarum strains and traditional starter culture Lactobacillus acidophilus NCIM2902. Using the central composite design approach, processing parameters were optimized for enhanced B2 content and probiotic count. Six independent variables were assessed: temperature (A: 35–45 °C), pH (B: 4–6), time (C: 3–18 h), and inoculum size for strains L. plantarum MTCC 25432 (D: 1–2%), L. plantarum MTCC 25433 (E: 1–2%), and L. acidophilus NCIM 2902 (F: 1–2%). The second-order model effectively predicted responses, identifying optimal fermentation conditions for developing vitamin B2-enriched soymilk: temperature (A) 36 °C, pH (B) 5.5, fermentation time (C): 11 h, inoculum size for L. plantarum MTCC 25432 (D): 2%, MTCC 25433 (E): 2%, and L acidophilus NCIM (F): 0.43%. These conditions resulted in a threefold increase in B2 concentration (481 µg/L) while maintaining a probiotic count of 9 logs CFU/mL. Additionally, techno-functional characterization, including rheology and texture profile analysis, showed that enhanced protease activity of co-cultured LAB improved protein hydrolysis (6259 nm), positively impacting the water holding capacity (WHC) and overall acceptability of the fermented soymilk. This optimized fermentation process represents a novel approach to developing nutritionally enhanced dairy-free soy products with high riboflavin content, utilizing the synergistic benefits of co-fermentation by two riboflavin-producing L. plantarum strains and traditional starter culture of L. acidophilus. This advancement is particularly significant for lactose-intolerant and vegan consumers who may lack sufficient dietary sources of Vitamin B2.

Evaluation of Genetic Diversity of Black Soybean [Glycine max (L.) Merr] by Using RAPD and ISSR Markers.

<b>Background and Objective:</b> Black soybeans [<i>Glycine max</i> (L.) Merr] are among the important crops, but the cultivated resources are normally low-yielding, susceptible to diseases and low profit. Therefore, it is necessary to evaluate the genetic diversity of black soybean germplasms for breeding programs. This study investigates the genetic diversity of 22 black soybean varieties by RAPD and ISSR markers. <b>Materials and Methods:</b> Twenty two black soybean genotypes in Vietnam were evaluated for genetic diversity by using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Data were scored following a binary matrix and analyzed using NTSYSpc 2.1 (Numerical Taxonomy and Multivariate Analysis System). <b>Results:</b> All 20 RAPD and 11 ISSR markers produced scorable bands. As 230 loci were investigated over the population, of which 107 were polymorphic, accounting for 46.5%. The collection of 22 black soybean varieties had a relatively close relationship with high genetic similarity coefficients, ranging from 0.71-0.99. Two main genetic clusters were classified. The RAPD markers showed better performance than ISSR markers in evaluating the genetic diversity of these 22 black soybean varieties. <b>Conclusion:</b> The results of this study display that 22 Vietnamese black soybean varieties are relatively identical in genetics. The study is suitable for breeding programs to improve black soybean varieties.

Biological Activities and Mechanisms of Actions of Bioactive Compounds (BACS) Identified in Pure and Ternary Blends of Cocoyam, Soya Bean and Bambara Groundnut Flour Using Gas Chromatography-Mass Spectrometry (GC-MS) Technique: A Review

Background: functional foods contain bioactive compounds (BACS) and can be sourced from both animals and plants like cocoyam (CY), soya bean (SB) and bambara groundnut (BGN). previous studies have reported various bacs in each of these plants but literature is scanty on the types and amount, of bacs in such flour blends and formulations. when in combinations as blend, anti-oxidant, interactions occur, resulting in either potentiation, additive, synergistic or antagonistic effect, depending on several various variables. their in-vivo bioactivities which are due to various structural features of the BACS, can be antioxidant, anti-inflammatory, hypocholestrolaemic, antimicrobial and anti-diabetic activities. in this review the various bacs identified by gas chromotagraphy (GC)-mass spectrometry (MS) technique in the various formulations of CY-SB-BGN flour blends were researched for their biological activities and reported mechanisms of actions. Methodology: a google search of a study on bacs identified using gc-ms in cy-sb-bgn flour blend was conducted and one paper was identified. the bacs reported in the study was noted and literature search of the various biological activities of such compounds and their mechanisms of actions were conducted. the results were documented and discussed. Results and Discussion: the study revealed that thiadiazole, stilbene, aziridine, thiourea, amphetamine/phenylethylene, artemisinin, monoterpenoids, naphthalenes, i,4-diazpanes, phenols and flavanoids were the identified bacs in cy-sb-bgn flour blend formulations with both the pure and ternary blends showing different family classifications of compounds and different types of compounds in one family class. the biological activities and mechanisms of actions included antimicrobial, antioxidant, antiinflammatory and antidiabetic activities with reported mechanisms of actions for each of these invivo activities. Conclusion: there are bacs in cy-sb-bgn formulations which are reported from studies to possess anti-microbial, anti-inflammatory, anti-oxidant and anti-diabetic properties invivo, with very established mechanisms of actions, making the use of such plant foods in disease management scientific, hence the recommendation of such plant foods use as adjunct in chronic disease management. Keywords: Bioactive compounds, Plant foods, Cocoyam, Soya Bean, Bambara Groundnut

Overexpression of the GmPM35 Gene Significantly Enhances Drought Tolerance in Transgenic Arabidopsis and Soybean

Drought stress is one of the major adversity stresses affecting soybean (Glycine max [L.] Merr.) yield. Late embryogenesis abundant protein (LEA protein) is a large family of proteins widely distributed in various types of organisms, and this class of proteins plays an important role in protecting proteins, membrane lipids, and lipids inside the cell. The soybean GmPM35 gene is a member of the LEA_6 subfamily. The expression of the GmPM35 gene was significantly increased after drought stress in soybeans. A subcellular localization assay confirmed that the gene acts on the cell membrane. Against wild-type Arabidopsis thaliana, we found that Arabidopsis lines overexpressing the GmPM35 gene were significantly more drought-tolerant at germination and seedling stages under drought stress. To further investigate the drought tolerance function of this gene in soybeans, nine overexpression lines of the T3 generation soybean GmPM35 gene and two editing lines of the T3 generation soybean GmPM35 gene were obtained by Agrobacterium-mediated method using a wild-type soybean strain (JN28) as a receptor. Germination rate, root length, chlorophyll (CHL) content, Proline (Pro) content, malondialdehyde (MDA) content, superoxide anion (O2−) content, hydrogen peroxide (H2O2) content, (NBT, DAB) staining, and activities of antioxidant enzymes (CAT, SOD, POD), and photosynthetic physiological indexes of the three different types of strains were measured and analyzed before and after drought stress. Combined with the results of rehydration experiments and physiological and biochemical indices, we found that overexpression of the GmPM35 gene protected the activities of antioxidant enzymes under drought stress. The activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were increased by an average of 34.28%, 26.12%, and 30.01%, respectively, in soybean plants overexpressing the GmPM35 gene compared with wild-type soybeans. Under drought stress conditions, soybean plants overexpressing the GmPM35 gene showed an average increase of 76.81% in photosynthesis rate (Pn), 39.8% in transpiration rate (Tr), 126% in stomatal conductance (Gs), 47.71% in intercellular CO2 concentration (Ci), and 26.44% in instantaneous water use efficiency (WUEi). The improvement of these indexes helped to reduce the accumulation of reactive oxygen species (ROS) in the plants. In addition, we found that under drought stress, the MDA content was reduced by an average of 18.8%, and the Pro content was increased by an average of 60.14% in soybean plants overexpressing the GmPM35 gene, and the changes in these indexes indicated that the plants had stronger antioxidant and osmoregulatory capacities in response to drought stress. In summary, this experiment demonstrated that the GmPM35 gene plays an important role in soybean tolerance to drought stress, and by overexpressing the GmPM35 gene, soybean plants can better tolerate drought stress and maintain normal physiological functions.

Chemical Fertility of a Ferralitic Soil in a Cropping System Based on Cereals (Fonio and Sorghum) and Legumes (Soya and Mung Bean) on An Experimental Station in Burkina Faso

In Burkina Faso, the low nutrient content of the country's soils has a direct impact on crop yields. The objective of this study is to evaluate the effects of applying organic matter (compost, poultry manure.), and mineral fertilizer Nitrogen-Phosphorus-Potassium (N:14% P:23% K:14%), urea (43% nitrogen) and Burkina Phosphate (BP) on soil fertility in a legume/cereal cropping system. The study was conducted at the INERA Farako-Bâ experimental station from 2018 to 2021. The experimental design was a 4x4 factorial, corresponding to four rotations and four manures, respectively, used as the first and second factors. A split-plot design with three replications was employed. The results obtained indicated that, in comparison to the initial soil, there was an increase of 33% in soil organic carbon and 36% in total nitrogen in the plots where soya and fonio were cultivated in rotation. In comparison to the plots with fonio-sorghum rotation, the plots with mung bean-sorghum and soya-fonio rotations demonstrated increase in assimilable phosphorus of 17% and 15%, respectively. Furthermore, the fertilizer combining compost and poultry manure resulted in a 300% increase in assimilable phosphorus and 28% increase in available potassium compared to the soil without fertilizer inputs and uncultivated for years. Additionally, the fertilizers of compost combined with poultry manure and compost combined with poultry manure and BP resulted in increases in assimilable phosphorus of 131.78% and 116%, respectively. The positive effects obtained on the chemical parameters (phosphorus, potassium and carbon) provide the potential for sustainable soil fertilization in cropping systems, while also valorizing local organic fertilizer.

Post‐rice soybeans and peanuts differ in performance, water use, and water‐use efficiency in the rainfed lowlands

AbstractThis study examined patterns of water use and water‐use efficiency in soybeans (Glycine max) and peanuts (Arachis hypogaea), for which data are rare, to assess their suitability as post‐rice (Oryza sativa L.) crops in the rainfed lowlands. A field experiment was conducted at Pakse in southern Lao PDR, comprising two irrigation regimes (weekly and as needed), three surface mulch treatments (nil, straw, and plastic), and two species (soybeans, peanuts), in a split–split design with three replicates. Volumetric soil water content was measured using time‐domain reflectometry. Water deficit was more severe under as needed irrigation, Nil cover, in soybeans, and late in the life cycle. Water use was highest in soybeans under nil cover and weekly irrigation (432 mm) and was lowest in peanuts under plastic cover and as needed irrigation (225 mm). Soybeans were responsive in plant height (41.5–62.7 cm), while peanuts were responsive in grain yield (1.05–1.81 Mg ha−1). Water‐use efficiency was lowest in soybeans under nil cover and weekly irrigation (2.48 kg ha−1 mm−1), and highest in peanuts under plastic cover and as needed irrigation (8.04 kg ha−1 mm−1). The paper concludes that peanuts should have a role as a post‐rice crop in the rainfed lowlands, with access to supplementary irrigation.

The members of zinc finger-homeodomain (ZF-HD) transcription factors are associated with abiotic stresses in soybean: insights from genomics and expression analysis

BackgroundZinc finger homeodomain (ZF-HD) belongs to the plant-specific transcription factor (TF) family and is widely involved in plant growth, development and stress responses. Despite their importance, a comprehensive identification and analysis of ZF-HD genes in the soybean (Glycine max) genome and their possible roles under abiotic stress remain unexplored.ResultsIn this study, 51 ZF-HD genes were identified in the soybean genome that were unevenly distributed on 17 chromosomes. All GmZF-HD genes contained a conserved ZF-HD_dimer domain and had diverse physicochemical features. Furthermore, the GmZF-HD gene structures exhibited 3 to 10 conserved motifs, and most of them showed intronless gene structures. Phylogenetic analysis categorized them into eight major groups with the highest closeness to dicots including Brassica rapa and Malus domestica. The cis-element analysis recognized plant growth and development (10%), phytohormones (31%) and stress-responsive (59%) elements. Synteny analysis identified 73 segmental and 1 tandem duplicated genes that underwent purifying selection. The collinearity analysis revealed that GmZF-HD genes showed higher homology with dicot species, indicating common ancestors with close evolutionary relationships. A total of 94 gma-miRNAs from 41 diverse miRNA families were identified, targeting 40 GmZF-HD genes, with GmZF-HD6 being most targeted by 7 miRNAs, and gma-miR4993 emerging as the dominant miRNA family. Different TFs including ERF, LBD, BBR-BPC and MYB, etc., were predicted in all 51 GmZF-HD genes upstream regions and visualized in the network. Expression profiling through RNA-Seq showed diverse expressions of GmZF-HD genes in different tissues including seeds, roots, shoots and leaves under diverse conditions. Further, the qRT-PCR analysis demonstrated that all tested GmZF-HD genes were significantly induced in soybean leaves, mainly the GmZF-HD5/6/13/39 and GmZF-HD45 genes were significantly upregulated (2.5 to 8.8 folds) under the tested stress treatments compared to control, highlighting their potential roles in response to stresses in soybean.ConclusionOverall, this study reveals comprehensive insights into the ZF-HD genes in soybeans and provides a valuable contribution towards functional studies for soybean improvement under stress conditions.

Enhancing Soybean Salt Tolerance with GSNO and Silicon: A Comprehensive Physiological, Biochemical, and Genetic Study

Soil salinity is a major global challenge affecting agricultural productivity and food security. This study explores innovative strategies to improve salt tolerance in soybean (Glycine max), a crucial crop in the global food supply. This study investigates the synergistic effects of S-nitroso glutathione (GSNO) and silicon on enhancing salt tolerance in soybean (Glycine max). Two soybean cultivars, Seonpung (salt-tolerant) and Cheongja (salt-sensitive), were analyzed for various physiological, biochemical, and genetic traits under salt stress. The results showed that the combined GSNO and Si treatment significantly improved several key traits, including plant height, relative water content, root development, nodule numbers, chlorophyll content, and stomatal aperture, under both control and salt stress conditions. Additionally, this treatment optimized ion homeostasis by enhancing the Na/K ratio and Ca content, while reducing damage markers such as electrolyte leakage, malondialdehyde, and hydrogen peroxide. The stress-responsive compounds, including proline, ascorbate peroxidase, and water-soluble proteins, were elevated under stress conditions, indicating improved tolerance. Gene expression analysis revealed significant upregulation of genes such as GmNHX1, GmSOS2, and GmAKT1, associated with salt stress response, while GmNIP2.1, GmNIP2.2, and GmLBR were downregulated in both varieties. Notably, the salt-sensitive variety Cheongja exhibited higher electrolyte leakage and oxidative damage compared to the salt-tolerant Seonpung. These findings suggest that the combination of GSNO and silicon enhances salt tolerance in soybean by improving physiological resilience, ion homeostasis, and stress-responsive gene expression.

Environmental impact of lead in soybeans (Glycine max L.) marketed in the canton of Quevedo, Ecuador

The bioaccumulation of lead in soybean crops, originating in contaminated soils, represents a significant environmental problem and a risk to public health due to the inherent toxic metal. The objective of this study was to evaluate the environmental impact of lead in samples of soybeans (Glycine max L.) marketed in the canton of Quevedo, Ecuador. Grain samples were collected randomly, in triplicate, from four local markets during a period of [indicate period] and a control sample from a non-contaminated cultivation area. The samples were subjected to a preparation process that included drying, grinding, acid digestion with concentrated HNO₃ and HCl, and dilution. Lead determination was carried out by electrothermal atomization atomic absorption spectrometry (GFAAS). The calculation of the lead contamination index was performed using a standardized equation, contrasting the results obtained with the maximum allowable limits established by national (NTE INEN 452:2013) and international (Codex Alimentarius, European Union) regulations. The results evidenced an average concentration of lead in the soybean samples of 1.15 ± 0.13 mg.kg-1, significantly exceeding the maximum allowable limits. The calculated contamination index indicated a high level of contamination in the analyzed grains, which represents a risk to both the ecosystem and human health when consumed. These findings underscore the need to implement environmental and food management strategies to reduce lead contamination in soybean crops, thus ensuring food safety and public health protection.

Calcium and boron, with and without amino acids, in foliar fertilisation in soybean

ABSTRACT The aim was to verify the viability of foliar fertilisation of calcium (Ca) and boron (B) in soybeans (Glycine max) in soils with various fertility levels. The experiment was carried out in four agricultural seasons and designed in randomised blocks, with three treatments and seven replications. The treatments were as follows: control, and Ca and B spraying on soybean plants without and with amino acids. Spraying was carried out at the flowering of the crop one time. It was concluded that there were significant differences in the yield components in the two crops, when the experiment was installed in soils with lower fertility, in which the foliar fertiliser with amino acid stood out. When there was a significant difference in productivity, the B content was altered; the value in the grains that received the product with amino acids was greater than the treatment without amino acids and above the treatment in which the plants were not sprayed with foliar fertilisers. Therefore, we highlight that Ca and B foliar fertilisation is especially required for soils with low CEC and Ca contents. However, this technique will likely not improve soybean performance for soils with no nutrient deficiency.

Шаманские идолы «человеко-бабочки» в проекции советской внешней политики на Дальнем Востоке во второй половине ХХ в.

The purpose of the study is to trace the transformation of shamanic idols of the Amur peoples under the influence of Soviet foreign policy in the Far East in the second half of the 20th century. The scientific novelty of the article is to identify the connection between the development of international relations in the Soviet Far East in the second half of the 20th century and changes in the appearance and religious purpose of some samples of cult sculpture of the Amur peoples. The article reveals that in the second half of the 20th century, when international contacts with Japan and especially with China intensified, some shamanic idols of the Amur peoples acquired attributes associated with Japanese and Chinese shamanism, in particular wings made from black soybeans, which in Japan are used for the ritual of "exorcism". The results of the study showed that such borrowings are natural in conditions when the peoples of different states have common cultural roots, and the results of foreign policy can have religious "side effects".

GmGIF5 Promotes Cell Expansion by Negatively Regulating Cell Wall Modification

Soybean is an important and versatile crop worldwide. Enhancing soybean architecture offers a potential method to increase yield. Plant-specific transcription factors play a crucial, yet often unnoticed, role in regulating plant growth and development. GRF-INTERACTING FACTOR (GIF) genes are plant-specific transcription factors; however, their functions in soybean remain poorly understood. Eight GmGIF members were identified in soybean (Glycine max L.). Phylogenetic analysis divided the eight GmGIF proteins into three groups. In this study, we focused on the role of GmGIF5 owing to its high expression level in the meristem. Subcellular localization and transcriptional activity analysis showed that GmGIF5 was localized to the nucleus and has self-transactivation ability. To elucidate the biological function of GmGIF5, we constructed transgenic Arabidopsis lines overexpressing the gene. Phenotype observations indicated that the overexpression of GmGIF5 contributed to larger leaves, higher plants, wider stems, and larger seeds. The organs of GmGIF5 overexpression lines exhibited larger sizes primarily due to an increase in cell size rather than cell number. RNA sequencing was performed to investigate the underlying mechanism for these effects, showing that differentially expressed genes in overexpression lines were mainly enriched in cell wall modification processes. Our study provides new clues for an understanding of the roles of the GmGIF family in soybean, which can promote the further application of these genes in genetic breeding.

Techno-functional properties of soy, rice and carioca bean protein blends

Techno-functional properties of soy, rice and carioca bean protein blends

Genome-Wide Identification and In-silico Expression Analysis of Serine Carboxypeptidase-Like (SCPL) Proteins in Soybean (Glycine max L.)

The serine carboxypeptidase-like (SCPL) protein family is crucial in plant development and stress response regulation. However, information on SCPL proteins in soybean remains limited. Here, we identified 84 GmSCPL genes distributed across 19 chromosomes. Phylogenetic analysis grouped GmSCPLs into three clusters, closely related to SCPLs in Arabidopsis thaliana and Oryza sativa. Duplication analysis revealed 31 duplicated gene pairs, with 27 linked to whole-genome or segmental duplication and 4 to tandem duplication. Three hub genes, GmSCPL82, GmSCPL46, and GmSCPL16 were identified through protein-protein interaction analysis. Promoter regions predominantly contain cis-regulatory elements for light, hormone, environmental, and growth responses. RNA-seq data highlighted GmSCPL20, GmSCPL33, and GmSCPL76 as highly up-regulated under heat stress; GmSCPL40, GmSCPL64, and GmSCPL69 under drought; and GmSCPL2, GmSCPL27, and GmSCPL37 under salt stress. This study conducted a comprehensive analysis of the SCPL protein family in soybeans, offering a valuable foundation for future research on the role of SCPL genes in abiotic stress tolerance. Plant Tissue Cult. & Biotech. 34(2): 217-235, 2024 (December)