Soy Protein Sources Research Articles

135 Effects of altering diet acid-binding capacity-4 (ABC-4) with specialty soy protein sources or acidifiers on nursery pig performance and fecal dry matter

Abstract A total of 300 pigs (initial body weight = 6.0 kg) were used to evaluate the effects of altering diet acid-binding capacity-4 (ABC-4) with specialty soy protein sources or acidifiers on nursery pig performance and fecal dry matter (DM). At weaning, pigs were allotted to 1 of 5 dietary treatments. There were 5 pigs/pen and 12 replications/treatment. Pigs were fed experimental diets in two phases with phase 1 fed from d 0 to 10 post-weaning followed by phase 2 from d 10 to 24. A low ABC-4 diet was formulated to 200 and 250 meq/kg in phase 1 and 2, respectively. The low ABC-4 diet utilized 0.38% fumaric acid, 0.36% formic acid, and specialty soy protein concentrate (AX3 Digest; Protekta; Newport Beach, CA) at 9.38 and 7.50% of the diet in phase 1 and 2, respectively. For the first medium ABC-4 diet, specialty soy protein concentrate was replaced with enzymatically treated soybean meal (HP 300; Hamlet Protein; Findlay, OH) on an SID Lys-basis and resulted in an ABC-4 of 290 and 322 meq/kg for phase 1 and 2, respectively. For the second medium ABC-4 diet, acidifiers were removed resulting in an ABC-4 of 271 and 321 meq/kg for phase 1 and 2, respectively. In the high ABC-4 diet, both soy protein replacement and acidifier removal strategies were utilized resulting in ABC-4 values of 362 and 394 meq/kg for phase 1 and 2, respectively. In addition, a control diet was utilized with the same formulation as the high ABC-4 diet but with 3,000 and 2,000 mg/kg of Zn from ZnO in phase 1 and 2, respectively. Following phase 2, all pigs were fed a common diet until d 38 of the study. Increasing ABC-4 decreased (linear, P ≤ 0.043) gain to feed ratio (G:F) during the experimental period and overall (Table). Increasing ABC-4 decreased (linear, P ≤ 0.005) fecal DM on d 10 and 24, with no differences on d 17. Pigs fed the medium ABC-4 diet based on specialty soy protein replacement had increased (P = 0.003) fecal DM on d 10 compared with the medium ABC-4 diet where acidifiers were removed. Pigs fed diets containing pharmacological levels of Zn from ZnO had increased (P ≤ 0.047) body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), and G:F during the experimental period but decreased (P = 0.005) ADG and G:F during the common period, with no differences overall compared with pigs fed diets not containing ZnO. In conclusion, as dietary ABC-4 increased from 200 to 362 meq/kg in phase 1 and 250 to 294 meq/kg in phase 2, growth performance and fecal DM decreased. This study suggests a dietary ABC-4 of 200 and 250 meq/kg in phase 1 and 2, respectively, can be utilized to improve growth performance and fecal dry matter of weanling pigs.

Evaluation of textural and sensorial properties of burger patties produced with plant-based protein alternatives

Recently, there has been a growing interest in alternative protein sources. This study aimed to determine the textural and sensory properties of vegan burger patties produced with plant-based (soy and pea) protein sources instead of meat protein. For this purpose, 14 different vegan burger patties produced with soy and pea-based protein alternatives were obtained from different brands in the İstanbul market. Burger patties were analysed in terms of moisture content, cooking properties, instrumental colour, texture profile, and sensory properties. According to the results, pea-based burger patties have a higher moisture content and cooking loss and a lower cooking yield than soy-based patties. The L* and a* values of the pea-based patties tended to be higher than those of the soy-based patties, while the b* values were generally higher. In addition, the texture profile analysis of soy and pea-based burger patties shows differences in texture attributes that may affect consumer preference and overall eating experience. In the sensory evaluation, a significant difference between soy and pea-based burger patties was observed only for juiciness and spicy taste characteristics. The research findings shed light on alternative proteins' textural and sensorial properties and their role in the food industry, particularly in developing vegan burger patties. These differences in sensory attributes and colour and texture analysis between soy and pea-based burger patties highlight the importance of ingredient selection and formulation in developing plant-based protein products.

386 Effects of Different Soy Protein Sources on Gut Microbiome Composition and Relationship with Performance in Pigs in Regular Nursery and F4 Enterotoxigenic Escherichia Coli (ETEC) Challenged Conditions

Abstract Provisoy, produced by hydro, thermal, and mechanical process using soybean meal (SBM) has been shown to improve both post-weaning pig growth and gut health compared with SBM. It was hypothesized that Provisoy could improve the utilization of both protein and fiber fractions along the gastrointestinal tract of a nursery pig resulting in a more balanced nutrient environment and robust microbiome in the hindgut compared with SBM. A total of 268 weaned pigs (body weight, 6.82 ± 0.85 kg) were allotted to either a regular nursery condition (5 pigs/pen, 10-11 pens/treatment) or subjected to ETEC challenge (3 pigs/pen, 12 pens/treatment, 1 pig per pen was orally gavaged with O149:K91:K88 strain on d 5 post-weaning). Pigs received one of the following diets: SBM, Provisoy or HP300 (soy protein from Hamlet Protein, used as a benchmark). A standard wheat-barley-SBM based diet was used, and test soy products were added to replace SBM in the treatment diets during the first three weeks of nursery program. All diets were isocaloric with the same standardized ileal digestible lysine level in a three-phase (38 days) nursery program. Fecal samples were collected on d 13 or d 14 post-weaning and subjected to DNA extraction and Nanopore shotgun sequencing. Data were center-log-ratio transformed and subjected to statistical analysis. Microbiome composition was affected by dietary treatment (P < 0.01) and challenge (P < 0.05), but their interaction was not significant. In regular nursery condition, both Provisoy and HP300 tended (P < 0.10) to have greater microbiome alpha-diversity compared with SBM, but no difference was observed between these two treatments. Only SBM fed pigs subjected to ETEC challenge had greater (P = 0.02) alpha-diversity compared with their non-challenged counterparts. Compared with the SBM treatment, pigs that received Provisoy in the regular condition had greater abundance of Coprococcus and Dialister, which were positively correlated with greater average daily gain (ADG; r = 0.53, P < 0.01, and r = 0.45, P = 0.01, respectively). Under ETEC challenge, an increase in abundance of Dialister was observed in pigs that received Provisoy treatment with a positive correlation with ADG (r = 0.42, P = 0.01). In conclusion, dietary inclusion of Provisoy or HP300 improved microbiome alpha-diversity compared with SBM. Provisoy helped promote the growth of beneficial bacteria in the hindgut of nursery pigs which were positively correlated with gain.

121 Use of Specialty Soy Products as Alternatives to Poultry Meal and Spray-Dried Blood Plasma in Nursery Pig Diets

Abstract A total of 2,260 pigs (initial BW = 6.7 ± 0.3 kg) were used to evaluate specialty soy protein sources as alternatives to poultry meal and spray-dried blood plasma on nursery pig performance. At weaning, pigs were allotted to 1 of 5 dietary treatments based on initial weight in two research nurseries. In the first facility there were 20 pigs per pen and 10 pens per treatment. In the second facility, there were 21 pigs per pen and 12 replications per treatment for a total of 22 replications per treatment. Pigs were fed experimental diets during phase 1 (d 0 to 7) and phase 2 (d 7 to 21) followed by a common diet for an additional 21 days (d 21 to 42). Dietary treatments included a control diet containing 9.5% poultry meal (AV-E Digest, XFE Products, Des Moines, IA) and 4.13 (Phase 1) or 2.75% (Phase 2) spray-dried blood plasma (Appetein, APC Inc., Ankeny, IA) and a 2 × 2 factorial with a novel soy protein concentrate (AX3 Digest; Protekta; Plainfield, IN) or microbial enhanced soy protein (MEPro; Prairie Aquatech; Brookings, SD) replacing poultry meal or poultry meal and spray-dried blood plasma. Protein sources were added to the diet to maintain a similar level of soybean meal and balance for SID amino acids. Thus, soy protein sources were added to the diet at 6 and 13 (Phase 1) or 10.75% (Phase 2) to replace poultry meal or poultry meal and spray-dried blood plasma, respectively. Dietary treatment structure allowed the opportunity to compare each protein source to each other, soy protein sources as a replacement to poultry meal, and soy protein sources as a replacement to spray-dried blood plasma. During the experimental period (d 0 to 21), pigs fed novel soy protein concentrate had improved (P < 0.001) G:F with no differences (P > 0.10) in ADG or ADFI compared with pigs fed microbial enhanced soy protein (Table). Pigs fed either soy protein as a replacement of poultry meal had increased (P < 0.001) ADG and ADFI compared with pigs fed the control diet. Additionally, pigs fed either soy protein source as a replacement of spray-dried blood plasma had improved (P = 0.044) G:F compared with pigs fed either soy protein without replacing spray-dried blood plasma, with no differences (P > 0.10) in ADG or ADFI. In summary, utilizing a specialty soy protein source as a replacement for poultry meal improved growth performance. Further replacing poultry meal and spray-dried blood plasma with the specialty soy proteins improved feed efficiency during the experimental period. The novel soy protein concentrate improved feed efficiency compared with microbial enhanced soy protein during the experimental period with no effect on ADG or ADFI.

Effect of Dietary Soy Protein Source on Effluent Water Quality and Growth Performance of Rainbow Trout Reared in a Serial Reuse Water System.

Juvenile rainbow trout (125 ± 0.8 g) were fed a fishmeal control diet (C), a high soy protein concentrate diet (SP), a high soybean meal diet (HiS), or a diet with high levels of fermented soy protein concentrate (fSP) for 12 weeks in a tank system capable of receiving 1st and 3rd use water from a serial-reuse production hatchery. Water quality was generally lower in 3rd use compared to 1st use water and after passing through tanks (inflow vs. outflow). Total dissolved solids were significantly higher (p = 0.003) for 3rd use compared to 1st use water, and values were also higher (p < 0.001) for the fSP diet. Turbidity and ammonia were highest in tanks for trout fed the HiS and fSP and SP and fSP diets, respectively, but were characterized by high variation, which likely prevented the detection of significant differences. Weight gain (p < 0.001) and survival (p = 0.008) were significantly lower for trout in 3rd use compared to 1st use water. Trout fed the HiS diet were generally in poorer physiological condition with lower body fat stores (p = 0.05) and lower growth rate (p < 0.001) and survival (p = 0.05) compared to the other diets, which were similar. The expression of several stress-associated genes (FK506, DIO2, REGPS, Cyp1a, G6PH, GADD45a, and IRF-1) in the liver and gill showed that diet and water source affected their regulation. Replacement of FM by SP providing 50% of dietary protein promotes acceptable growth performance compared to an FM diet and was superior to HiS. The impacts of soy protein concentrate on water quality under commercial production conditions, however, require further study.

Porcine digestible peptides as alternative protein source in weaner diets: effects on performance and systemic cytokine profile in pigs followed from weaning to slaughter

Porcine digestible peptides (PDP) are high-quality hydrolysed proteins obtained from porcine intestinal mucosa as a by-product of the heparin manufacturing process. PDP contain bioactive peptides and are used as alternative protein sources in several animal species, including pigs. We aimed to explore the (carry-over) effects of feeding PDP to weaned piglets on performance and systemic cytokine levels of pigs followed until slaughter. A total of 192 piglets were allocated to one of two dietary treatments: control (CON) or PDP weaner diets. PDP was included at 5.0% until day 13 post-weaning at the expense of skimmed milk powder and partial replacement of soybean meal, and at 2.5% between days 13 and 34 post-weaning at the expense of soy protein concentrate. Grower-finishers were fed commercial diets according to a 3-phase feeding scheme until slaughter, when carcass traits were determined. Six pigs were housed per weaner pen and eight per grower-finisher pen with 16 and 10 pens per treatment, respectively. Pigs were weighed at the start and at the end of each phase, and feed intake was recorded. Faecal consistency was recorded twice a week in the weaner facility. Ten pigs per treatment were sampled for blood at days 13, 34 and 69 post-weaning. We found that PDP-fed piglets had a higher feed intake in the first two weeks post-weaning compared to CON-fed piglets (+32 g/pig per day; P = 0.02). Moreover, piglets in the PDP group showed improved feed conversion between days 13 and 34 versus the CON group (1.36 vs 1.43; P = 0.03). Piglets that were fed with PDP in the weaner diets tended to grow faster in the grower-finisher period (+32 g/pig per day; P = 0.07), tended to reach slaughter age earlier (129.9 vs 131.5 days; P = 0.07) and had a lower dressing percentage at slaughter (76.3 vs 76.7%, P = 0.045) than piglets previously fed with CON. Additionally, PDP-fed piglets showed higher serum levels of pro-inflammatory cytokines interleukin (IL)-12 (P = 0.02), tumour necrosis factor-alpha (P = 0.02), interferon-gamma (P = 0.03) and IL-8 (at day 34 post-weaning, P = 0.06) as well as anti-inflammatory cytokines transforming growth factor-beta (P = 0.02), IL-4 (P = 0.04) and IL-10 (at day 34 post-weaning, P = 0.02). No significant differences among dietary treatments were observed regarding faecal consistency of weaned piglets and carcass weight, lean meat percentage, muscle depth, and back fat thickness at slaughter. We conclude that feeding PDP, as an alternative to conventional milk and soy protein sources, showed positive effects on pig performance, not only during the provisioning period but also thereafter into the grower-finisher phase.

Effects of Altering Dietary Acid-Binding Capacity-4 with Specialty Soy Protein Sources or Acidifiers on Nursery Pig Performance and Fecal Dry Matter

Effects of Altering Dietary Acid-Binding Capacity-4 with Specialty Soy Protein Sources or Acidifiers on Nursery Pig Performance and Fecal Dry Matter

Use of Specialty Soy Products to Replace Poultry Meal and Spray-Dried Blood Plasma in Diets Provided to Nursery Pigs Housed in Commercial Conditions

A total of 2,260 pigs (PIC TR4 × [Fast LW × PIC L02]; initially 14.8 lb) were used to evaluate a specialty soy protein source as an alternative to poultry meal and spray-dried blood plasma on nursery pig performance in a commercial environment. At weaning, pigs were allotted to 1 of 5 dietary treatments based on initial weight in two research nurseries. In the first facility there were 20 pigs per pen and 10 pens per treatment. In the second facility, there were 21 pigs per pen and 12 replications per treatment for a total of 22 replications per treatment. Dietary treatments included a control diet containing 9.5% poultry meal (AV-E Digest, XFE Products, Des Moines, IA) and 4.13 (phase 1) or 2.75% (phase 2) spray-dried blood plasma (Appetein, APC Inc., Ankeny, IA). The four additional diets were set up in a 2 × 2 factorial with a novel soy protein concentrate (AX3 Digest; Protekta; Plainfield, IN) or fermented soybean meal (MEPro; Prairie Aquatech; Brookings, SD) replacing poultry meal or poultry meal and spray-dried blood plasma in the control diet. Pigs were fed experimental diets during phase 1 (d 0 to 7) and phase 2 (d 7 to 21). Following phase 2, pigs were fed a common diet for an additional 21 d (d 21 to 42). During the experimental period (d 0 to 21), pigs fed the novel soy protein concentrate had improved (P < 0.001) F/G with no differences in ADG or ADFI compared to pigs fed fermented soybean meal. During the experimental period (d 0 to 21) and overall (d 0 to 42), pigs fed soy protein as a replacement to poultry meal had increased (P ≤ 0.016) ADG and ADFI compared to pigs fed the control diet. During the experimental period (d 0 to 21), pigs fed soy protein as a replacement to spray-dried blood plasma had improved (P = 0.044) F/G compared to pigs fed soy protein without replacing spray-dried blood plasma, with no differences in ADG or ADFI. In summary, utilizing a specialty soy protein source as a replacement for poultry meal improved growth performance. Replacing poultry meal and spray-dried blood plasma with soy protein improved feed efficiency when treatment diets were fed, but not overall. In addition, the novel soy protein concentrate improved feed efficiency compared to fermented soybean meal during the experimental period with no effect on ADG or ADFI.

Compositional and Functional Considerations for Bovine-, Caprine- and Plant-Based Infant Formulas

Breastmilk is the optimal source of nutrition for infants. However, in circumstances where breastfeeding is not possible or feasible, infant formula provides an essential alternative to fulfil the nutritional requirements of the developing infant. Traditionally, the manufacture of infant formula has involved utilisation of bovine milk as a base ingredient, formulated with other nutrients and bioactive ingredients to closely match the composition of human breastmilk. While it is the most widely available type of formula on the market, bovine-based infant formula is not suitable for all infants, and therefore alternatives such as those based on caprine milk, soy and rice protein are becoming increasingly available. This review provides a detailed examination of the composition of infant formula prepared from bovine milk, caprine milk, soy, and rice protein sources. Available literature on nutrient bio-accessibility and aspects of protein functionality relevant to infant formula is discussed.

An initial evaluation of fishmeal replacement with soy protein sources on growth and immune responses of burbot (Lota lota maculosa)

Burbot (Lota lota maculosa) are the only freshwater member of the cod family (Gadidae) and potentially a new species for aquaculture production. This “freshwater cod” species could provide diversification for freshwater salmonid and other aquaculture operations, given its desirable fillet quality and growth potential. Burbot nutrition research is necessary to further define dietary requirements and the potential of this species to utilize sustainable plant-based proteins. A 91-day feeding trial and subsequent 28-day pathogen challenge was conducted with juvenile burbot (26.8 g mean weight) to assess growth performance, survival, and immune parameters. Dietary treatments included a fishmeal control (CTL), and 25% and 50% fishmeal replacement with soybean meal (SBM) soy protein concentrate (SPC), and bioprocessed soybean meal (BSBM). Growth results indicated a difference in percent gain (PG; P = 0.015) and thermal growth coefficient (TGC; P = 0.014) among dietary treatments, with the fish fed the SBM-25 and CTL performing higher than other diets except for the SPC-25. Analysis of distal intestine samples revealed no discernable differences in lamina propria thickness (P = 0.489) or cellularity (P = 0.944), and the amount of connective tissue of the submucosa was found to be similar (P = 0.988). Immediately following the feeding trial, burbot were challenged with a virulent strain of Aeromonas sp. (A141) while remaining on their respective dietary treatments. At day 28 post-challenge, no diet-related effects were found in cumulative percent mortality (P = 0.170) or serum lysozyme activity (P = 0.715). The respiratory burst activity of isolated head kidney-derived leukocytes did not show treatment differences at pre- and post-challenge sampling (P = 0.857), but activity decreased post-challenge (P < 0.001). Overall, results indicate that 25% FM replacements with SBM and SPC are suitable in burbot diets and long-term feeding of soy-based proteins does not appear to cause distal enteritis, impair disease resistance, or affect innate immune function. Utilizing soy-based protein sources for commercial burbot culture will expand the use of sustainable plant ingredients in aquaculture and provide burbot producers with a cost-effective alternative to high fishmeal feeds.

Effects of a high-protein corn product compared with soy and canola protein sources on nutrient digestibility and production responses in mid-lactation dairy cows

An experiment was conducted to assess the effects of a novel and proprietary high-protein corn product [56% crude protein (CP)] relative to other common sources of protein on the lactation performance of dairy cows. Twenty-four Holstein cows (620 ± 47.7 kg of body weight, 111 ± 34 d in milk, 2.28 ± 0.46 lactations; mean ± standard deviation) were randomly assigned to treatment sequence in a replicated 4 × 4 Latin square design balanced for carryover effects. Cows were individually fed 1 of 4 diets with a different protein concentrate source during each 28-d period, including soybean meal (SBM), high-protein corn product (HPCP), soybean meal with rumen-bypass soy protein (SBMBP), and canola meal with rumen-bypass soy protein (CANBP). Diets were formulated for equal concentrations of CP and balanced to meet predicted lysine and methionine requirements. The SBM diet was formulated to provide 5.7% rumen-undegradable protein (RUP), whereas SBMBP and CANBP diets were formulated for 6.8% RUP to match HPCP. Data were analyzed using mixed models with the fixed effects of treatment, period, square, the interactions of treatment and period and of treatment and square, and the random effect of cow. The CANBP diet increased dry matter intake (DMI) compared with SBM and HPCP. Treatment affected milk yield, as SBMBP and CANBP increased yield compared with SBM, but HPCP decreased milk yield compared with all treatments. The HPCP diet reduced CP intake as a percent of total DMI and increased the CP content of orts, indicative of selection against HPCP. The HPCP diet also decreased apparent total-tract and CP digestibility, leading to less urine nitrogen excretion and greater fecal nitrogen output. The SBMBP and CANBP diets performed similarly in nearly every variable measured, except that SBMBP increased milk urea nitrogen. In conclusion, the HPCP diet reduced yield of milk and milk components, likely because of reduced apparent total-tract dry matter and CP digestibility.

The Effects of Miscanthus Grass as a Bedding Source and the Dietary Inclusion of Unheated, Low-Trypsin Inhibitor Soybeans on the Performance of Commercial Tom Turkeys Reared to Market Age

SUMMARY A study was conducted to evaluate bedding source and the inclusion of rolled, unheated soybeans on the growth performance of male turkeys when reared to market age. Pine shavings are the most common poultry bedding material, however recently, increased marketplace competition has significantly reduced its availability and necessitated the need for economical alternatives. Additionally, the development of new varieties of soybeans with low levels of antinutritional factors have created an opportunity for dietary inclusion with minimal processing. The objective of this study was to determine the efficacy of Miscanthus grass as an alternative to pine shavings litter and to evaluate 2 novel, low trypsin inhibitor soybean varieties as a potential ingredient for turkeys. Treatments were arranged in a 2 × 4 factorial design with main effects of litter type (Miscanthus grass or pine shavings) and dietary treatment. Diets were defined based on the soy protein source: LO (novel, unheated low oligosaccharide and trypsin inhibitor soybean), LT (novel, unheated low trypsin inhibitor soybean), SBM (commercial solvent extracted soybean meal), or CB (unheated conventional soybeans). Bedding type did not affect BW gain or feed intake at market, however, a tendency for improved FCR was observed when turkeys were raised on Miscanthus grass (P = 0.073). Turkey toms fed diets containing low trypsin inhibitor soybeans had improved BW gain and feed intake with a lower overall FCR compared to turkeys fed diets containing conventional raw whole soybeans. Therefore, there was improved digestibility of the novel soybean lines. Nutrient utilization of the novel low trypsin inhibitor soybeans, however, remained inferior to diets containing solvent extracted soybean meal, which yielded the heaviest BW and lowest FCR with similar feed intake to the low trypsin inhibitor soybean diets.

Effects of dietary soy isoflavones and soy protein source on response of weanling pigs to porcine reproductive and respiratory syndrome viral infection.

Porcine reproductive and respiratory syndrome virus (PRRSV) is the most prevalent disease of swine globally. Infection of weanling pigs with PRRSV leads to a complex immune response resulting in significant disease and decreased growth performance. Previous experimental evidence suggests that increasing concentrations of soybean meal in the diet of young pigs confer benefits in terms of growth performance and immune parameters. The objective of this experiment was to identify potential modes of action for this benefit, specifically the ability for soy-derived isoflavones (ISF) to confer immunological benefits to young pigs infected with PRRSV. Four dietary treatments differing in soy protein source (soy protein concentrate vs. enzyme-treated soybean meal) and ISF supplementation (none vs. 1,500 mg total ISF/kg) were fed; the control diet (CON) contained soy protein concentrate and no supplemental ISF. Weanling pigs (60 barrows, 21 d of age, 5.71 ± 0.44 kg) from a naturally Mycoplasma hyopneumoniae (Mh)-infected source herd were individually housed in disease containment chambers and provided ad libitum access to experimental diets for 7 d before receiving either a sham inoculation or a 9.28 × 103 50% tissue culture infective dose of PRRSV at 28 d of age (0 d postinoculation). A total of 5 experimental treatments included an uninfected group receiving the CON diet, plus four infected groups each receiving a different dietary treatment. Growth performance and rectal temperatures were recorded throughout the study, and blood was collected for quantification of serum PRRSV load, presence of anti-PRRSV antibodies, differential complete blood counts, cytokine concentrations, and T-cell immunophenotyping. Data were analyzed as a 2-way or 3-way ANOVA for all treatments including PRRSV-infected pigs, in addition to a single degree of freedom contrast to compare uninfected and infected pigs receiving the CON diet. PRRSV-infection reduced growth rate and efficiency compared with noninfected controls with minimal influences by ISF. Supplemental ISF reduced PRRSV-induced band neutrophilia and improved cytotoxic-to-helper T-cell ratios. These results suggest that ISF contribute to activation of adaptive immune system pathways and could benefit recovery from and clearance of PRRSV infections.

Influence of soybean protein source on growth performance and nutrient digestibility of piglets from 21 to 57 days of age

The effects of including soy protein concentrate (SPC) and soybean meal (SBM) of different origins in the diet on growth performance and nutrient digestibility of weanling pigs were studied in 2 experiments. In both experiments, pigs received in phase I (21–49 d of age) one of five diets that differed in the soy protein source used. Two diets were based on SPC from USA or Brazil (BRA) origin and the other three diets were based on SBM from USA, BRA or Argentina (ARG) origin. In phase II (49 to 57 d of age), all pigs received a common maize-SBM diet. Pre-planned orthogonal comparisons (SPC vs. SBM; USA vs. BRA SPC; and USA vs. BRA vs. ARG SBM) were conducted to detect differences among soy treatments. In experiment 1, growth performance was similar for piglets fed diets containing SPC than for piglets fed diets containing SBM. In phase I (P=0.067) and cumulatively (P=0.082), post-weaning diarrhea (PWD) was lower in pigs fed SPC than in pigs fed SBM. The inclusion of USA meal in the diet improved ADG (P<0.01) and G:F ratio (P<0.01) compared with the inclusion of South American meals. The coefficient of total tract apparent digestibility (CTTAD) of crude protein was higher in pigs fed SPC (P<0.05) than in pigs fed SBM. Nutrient digestibility was similar for both SPC diets but lower (P<0.001) in pigs fed BRA SBM than in pigs fed USA or ARG SBM. In experiment 2, growth performance of the pigs was not affected by the soy product used, but in phase I (P=0.062) and cumulatively (P=0.083), PWD was lower in pigs fed SPC than in pigs fed SBM. Also, pigs fed the USA and BRA SBM had lower incidence (P<0.05) of PWD than pigs fed the ARG SBM. No differences in the CTTAD were detected between diets based on SPC and diets based on SBM, or between SPC origins but nutrient digestibility tended to be higher (P<0.10) for the USA than for the South American SBM. The data indicate that the chemical composition and nutritive value of commercial soy products varied between sources and among commercial batches of SBM, affecting growth, PWD and nutrient digestibility of the piglets.

PCR Detection of Soy Protein in Ready to Eat Meat Doners

Addition of soy protein sources in food products is widely used because of their functional properties such as water binding, fat binding, beneficial effects on texture and emulsification capability and providing improved economy with increasing yield. However, the use of soy protein in food products causes economical disadvantages because of replacement of an expensive ingredient like meat with a cheaper ingredient like soy and health risks for the consumers as well. Soy is an important allergy source for sensitive consumers. Because of these reasons, the most recent meat products regulation of Turkish Food Codex has banned the addition of soy in doners since 2012, like several other countries. Detection of soy in food products is performed by detection of soy protein or soy DNA. Because DNA is more stable to processing, PCR methods are shown to be more reliable when used in processed foods. In our study, 50 doner samples were collected from various retail sales points. Twenty-five doner samples were collected before and 25 after the regulation was enacted. DNA was isolated from doner samples and PCR testing of these DNA extracts were performed. The detection results of the doner samples showed that any of the 25 samples collected after the regulation enacted did not contain soy ingredient while 3 of 25 sample (12%) collected before the regulation came into force contained soy.

Effect of Soy Protein Sources on Nursery Pig Performance

Effect of Soy Protein Sources on Nursery Pig Performance

A Survey of Soy Protein as a Novel Dry Strength Agent for Papermaking

In this paper, soy protein papermaking process conditions as dry strength agents were studied. The results show that a part of the three parts of soy protein and a mixed system with a cationic starch alone, or cationic starch, soy protein, as compared to dry strength additives will significantly increase the strength. The optimum conditions were: pulp types E, defatted soy protein, dry vacuum drying method is 105°C, adding ways to add the pulp, the best reaction time was 10min, the optimum dosage of 2%. Soy protein sources are widely readily available, and environmentally friendly and easily biodegradable, paper dry strength agents as a good material.

Evaluation of specialty soy protein sources on nursery pig performance (2014)

A 35-d growth trial was conducted to evaluate the effects of a new soy protein source, Nutrivance (TechMix, Stewart, MN), on nursery pig growth performance. Nutrivance is a modified soy protein produced via a proprietary process combining extraction and enzymatic treatment of soybeans. Pigs (n = 1,188, PIC 337 × 1050; initially 9.8 lb BW) were weaned at 21 d of age and allotted by weight to pens with 27 pigs per pen. Pigs were fed a common diet for 15 d before the start of the study. Pens of pigs (13.5 lb BW) were then allotted to 1 of 4 dietary treatments fed for 14 d followed by a common diet fed for 21 d. The 4 experimental treatments were a corn-soybean meal–based control diet, or a corn-soybean meal–based diet with either 8% Nutrivance, 8.65% HP-300 (Hamlet Protein, Findlay, OH), or 6.85% Soycomil P (SPC; Archer Daniels Midland Co., Decatur, IL). The diets were formulated to the same standardized ileal digestible lysine level with specialty soy protein products replacing a portion of soybean meal in the control diet to form the experimental treatments.

Effects of the amount and source of dietary protein on bone status in rats

This study examined the effects of the dietary amount and source of protein on bone status in rats. 140 male Wistar rats aged 8 weeks were randomly allocated to 4 groups (n = 35) fed normal-protein (NP, 10% richness) or high-protein (HP, 45% richness) diets based on whey protein (WP) or soy protein (SP) sources for 12 weeks. Plasma urea was 46% higher for the HP compared to the NP diet (p < 0.001). Urinary calcium was 65% higher for the HP compared to the NP and 60% higher for the WP compared to the SP diets (all, p < 0.001). Urinary pH was 8% more acidic in the HP compared to the NP diet (p < 0.001) and 4% in the WP compared to the SP diet (p < 0.01). The plasma osteocalcin concentration was 19% higher for the NP compared to the HP (p < 0.05) and 25% for the SP compared to the WP diets (p < 0.01). Femur ash, metaphyseal and diaphyseal cross-sectional, trabecular and cortical areas were 3% higher in the HP compared to the NP diet (all, p < 0.05). Femur diaphyseal periosteal and endocortical perimeters were also 3% higher in the HP compared to the NP diet (both, p < 0.01). Groups fed the SP diet showed 2% higher femur ash percentage, 7% higher calcium content (both, p < 0.001), and 3% higher diaphyseal cortical area and thickness (both, p < 0.05) than those fed the WP diet. Some interactions were found, such as the greater effects of the SP diet on decreasing the higher plasma urea concentration promoted by the intake of the HP diet (p < 0.001). Under adequate Ca intake, HP diets could better maintain bone properties than NP diets, even with increasing some acidity markers, which could be reduced by the intake of SP sources.

Fast Characterization of Industrial Soy Protein Isolates by Direct Analysis with Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

Industrial soy protein isolates (SPIs) due to differences in their processing conditions may differ both in composition and in degree of hydrolysis. As a result, they display different performance in food production and final food properties like consistency and taste. To address this issue, a fast, cheap, and simple method for screening and characterization is required. In this article, the successful analysis of soy protein isolates, a complex mixture of proteins with glycinin and beta-conglycinin as major components, by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is demonstrated. The preparation implements a fast extraction of the proteins from the raw SPI either under neutral or reducing conditions. The extracts are analyzed subsequently by MALDI-TOF-MS without further purification. Results of the two conditions are compared. Finally, different SPIs from different suppliers are analyzed and compared concerning their consistency. The method could be applied to other plant proteins and mixtures thereof. Since the composition and intactness of different subunits play important roles in functional properties of soy proteins, rapid methods for fingerprinting of different industrial soy protein sources will be valuable tools for successful product formulation.