Soxhlet Technique Research Articles

Potency of the Leaf, Stem and Root Extracts of Petiveria alliacea Against Selected Microorganisms

Infectious diseases have contributed immensely to the rate of death globally in the past decades due to increased resistance to synthetic drugs. Hence, there is an urgent need to find a lasting solution to the problem of drug resistance, through the emergence of alternative medicine. One of such plant with biologically – active components against variety of diseases is Petiveria alliacea. It has been used to reduce inflammations and pains and also to eliminate bacteria, fungi, candida and viruses. The potency of the oils obtained from the leaves, stems and roots of this plant through (Soxhlet and Cold extraction methods) were subjected to test against certain micro-organisms, such as: Staphylococcus aureus, Bacillus subtilis, Escherichia coli, as well as the anti-fungi test on Rhizopus sp., and Aspergillus niger. The cold hexane leaf extract and cold ethanol leaf extract were most effective at limiting the growth of Salmonella typhi and the Rhizopus sp. respectively with an MIC value of 3.125 μg/mL and MBC value of 6.25 μg/mL. The ethanol leaf extracts using Soxhlet technique were most effective at MIC value of 3.125 μg/mL on Staphylococcus aureus, Escherichia coli, Rhizopus sp., and Aspergillus niger. The ethanol leaf extracts using Soxhlet extraction technique were most effective at MIC value of 3.125 μg/mL on Staphylococcus aureus, Escherichia coli. Likewise, the hexane and ethanol cold leaf extracts were most effective in limiting the growth of Rhizopus sp. and Aspergillus niger with MIC value of 3.125 μg/mL and minimum fungicidal concentration (MFC) value of 6.25 μg/mL. The antioxidant property of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay with ascorbic acid as the reference standard.

Microwave-Assisted Extraction of Curcuma longa L. Oil: Optimization, Chemical Structure and Composition, Antioxidant Activity and Comparison with Conventional Soxhlet Extraction

Curcuma root (Curcuma longa L.) is a very important plant in gastronomy and medicine for its unique antiseptic, anti-inflammatory, antimicrobial and antioxidant properties. Conventional methods for the extraction of curcuma oil require long extraction times and high temperatures that can degrade the active substances. Therefore, the objectives of the present study were: (i) first, to optimize the extraction yield of curcuma oil by applying a Box-Behnken experimental design using surface response methodology to the microwave-assisted extraction (MAE) technique (the independent variables studied were reaction time (10–30 min), microwave power (150–200 W) and curcuma powder/ethanol ratio (1:5–1:20; w/v); and, (ii) second, to assess the total phenolic content (TPC) and their antioxidant activity of the oil (at the optimum conditions point) and compare with the conventional Soxhlet technique. The optimum conditions for the MAE were found to be 29.99 min, 160 W and 1:20 w/v to obtain an optimum yield of 10.32%. Interestingly, the oil extracted by microwave-assisted extraction showed higher TPC and better antioxidant properties than the oil extracted with conventional Soxhlet technique. Thus, it was demonstrated that the method applied for extraction influences the final properties of the extracted Curcuma longa L. oil.

Convective drying of Moringa oleifera seeds: kinetics modelling and effects on oil yield from different extraction techniques

This work shows the convective drying kinetics of moringa seeds (Moringa oleifera L) at different drying temperatures 40, 50, 60, 70, and 80 °C and the effect drying on the extracted oil yield. Four simplified models were fitted to the kinetics data, and a phenomenological mathematical model (PMM) was developed to describe the process. The simplified model that better represented the experimental data was Verma, with a global relative mean error of 1.31%. The PMM with the use of an equilibrium boundary condition at the surface produced better results than those obtained when considering the convective condition, indicating that the effects of external mass transfer resistance are neglected. The moringa oil was extracted from all dried seeds conditions using different extraction techniques, such as Soxhlet technique, ultrasound-assisted, and mechanical extraction; and the maximum oil yield obtained for dry seeds was 43.06%, 31.53%, and 24.93% respectively. All drying temperatures of the moringa seed do not show influence the oil yield obtained in each extraction method used.

Optimization of oil extraction from Lallemantia iberica seeds using ultrasound-assisted extraction

The present study aims at optimising the oil extraction from Dragon’s head (Lallemantia iberica) seeds using response surface methodology. For this purpose, the working conditions (solid/liquid ratio, ultrasound intensity and extraction time) were varied in a series of experiments to determine the optimum extraction conditions, which were further verified through an independent extraction experiment. The experiments were conducted using ultrasound-assisted extraction (UAE) technique. The maximum yield obtained for UAE extraction efficiency under optimal conditions was 97.02%, taking the Soxhlet technique as a reference (corresponding to an oil content of 39.73 g/100 g dry seeds). The operation conditions for maximum extraction efficiency were: 1/16 (g/mL) solid/liquid ratio, 13.77 (W/cm2) ultrasound intensity and 12.5 min extraction time.

Chemical Components and Antimicrobial Activity of Essential Oils of Petiveria alliacea Leaves Extracts

A steam distillation technique was employed to obtain oil from the leaf of P. alliacea. The oil obtained was subjected to GC/MS analysis to determine the chemical components, which showed the presence of sulphur heterocyclic compounds, 1,2,3-trithiolane (3), 1,2,5 trithiepane (4) and 1,2,5,6-tetrathiocane (7) as well as benzenecarbothioic acid (8) that have not been reported previously as components in the crude extracts of Petiveria alliacea. The crude extracts showed antimicrobial activity on the following microorganisms Salmonella typhi, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Rhizopus sp., Aspergillus niger. The tests showed that the extract was most effective at limiting the growth of Salmonella typhi and the Rhizopus sp. (MIC 3.125 µg/mL and MBC value of 6.25 µg/mL). The ethanol extract using the Soxhlet technique was the most effective on Staphylococcus aureus, Escherichia coli, Rhizopus sp., and Aspergillus niger (MIC 3.125 µg/mL).

ANTI ACNE CREAM EFFECTIVITY OF METHANOL EXTRACT OF Impatiens balsamina Linn. LEAVES

Many studies have found that Impatiens balsamina leaves contain antibacterial activity. The present study was conducted to evaluate antiacne effect from cream of methanolic extract from leaves of Impatiens balsamina L. towards Propionibacterium acnes and Staphylococcus epidermidis , which concentration that gives the most effective antiacne and to evaluate physical and chemical properties of creams. The simplicia was extracted by soxhlet technique and using methanol as solvent. Kirby-Baeur diffusion method was used to measure the antibacterial activity. The extract was formulated in cream with concentration of 10%, 15% and 20%. Evaluation of physical and chemical properties was done by organoleptic testing, spreadability, adhesive force, pH and antibacterial effectiveness. Data was analyzed using R-Commander program 2.14.1 Version. Formula II which contained 15% extract showed the most effective inhibiton to the growth of bacteria. The evaluation of FII cream showed that the cream was homogen, the spreadability was 21,24 + 1,464cm 2 , adhesive force more than 60 minutes, and pH of 6,53 + 0,057.

Added-value molecules recovery and biofuels production from spent coffee grounds

Spent Coffee Grounds worldwide production is estimated at around 6 M tons only at industrial level. The abundance and the heterogeneity of this substrate make it an ideal substrate for a biorefinery approach based on the “cascade biorefinery hierarchy”. Currently, the major part of spent coffee grounds is sent to incineration and landfill disposal, options which should be avoided. Instead, they could be valorised through biofuels production. All the operational parameters leading to the highest biogas (350-400LCH4/kgTVS), bioethanol (3–4%v/v) and biodiesel (over 90% of Fatty Acid Methyl Esters concentration) yields from spent coffee grounds have been discussed in this review paper. They are rich in an oil phase containing different added-value molecules (tocopherols, cafestol, kahweol along with linoleic and palmitic acids), which can be extracted and used as additives for food, cosmetic and pharmaceutical applications. Solid/liquid extraction techniques of coffee oil from spent coffee grounds such as the most common Soxhlet technique and the more innovative fluids in supercritical conditions have been discussed, with coffee oil recovery of around 5–15%w/w and 15–20%w/w, respectively. The most recent applications of the extracted coffee oil have been also presented: the added-value molecules recovery and purification after micro/ultra and nano filtrations processes and the polyhydroxyalkanoates (0.84 g/g) and biosurfactants (3.5 g/L) production. Considering the whole information, an integrated biorefinery scheme, along with the respective mass balances were proposed. The novelty of this paper lies in the integration of the state-of-the-art data, in a biorefinery concept that would allow the production of both biofuels and value-added products.

Chemical composition and biological properties of Synedrella nodiflora (L.) Gaertn: A comparative investigation of different extraction methods

Synedrella nodiflora (L.) Gaertn. (Asteraceae), is a weed with ethnomedicinal uses. To extend scientific information on this species, we evaluated the effects of different extraction procedures (maceration, Soxhlet, sonication and homogenizer-assisted extraction (HAE)) using methanol as solvent, on the chemical composition and biological potential. The chemical profiles of the extracts were identified using a chromatographic (UHPLC-HRMS) technique. The antioxidant and enzyme inhibitory activities of the studied extracts were determined. The extract obtained by Soxhlet technique showed a higher level of total phenolic (TPC) and flavonoid content (TFC) and was a superior source of antioxidant compounds. The macerated extract was the most potent inhibitor of cholinesterases and α-glucosidase, whereas the highest activity against tyrosinase was observed in the order of sonication > Soxhlet > HAE > maceration. A modest activity was observed against α-amylase for all the extracts. Multivariate analysis showed that the bioactive compounds recovery and the biological activities of S. nodiflora were mostly dependent on the nature of the extraction technique used. In conclusion, S. nodiflora extracts showed good biological potential and data massed from this study could serve as a scientific baseline for further investigation in order to exploit its potential for designing novel bio-products with therapeutic applications.

Rubber seed oil extraction: Effects of solvent polarity, extraction time and solid-solvent ratio on its yield and quality

Abstract Two solvents (isopropanol and n-hexane) with different polarity were employed in oil extraction from rubber seed with the aim of determining their effects on its yield and properties. The solvent extraction process deployed through Soxhlet technique was modeled and vital parameters affecting the characteristics of the rubber seed oil (RSO) were identified and optimized by D-Optimal design. Physical and chemical characteristics of the RSO were examined. The fatty acid content and functional groups in the RSO were investigated using gas chromatography and Fourier transform infrared (FT-IR) techniques, respectively. Optimum RSO yield of 63.14 wt% was observed in 2.7 h using solid:solvent ratio of 0.05 g/mL and n-hexane (a non-polar solvent). Maximum RSO yield obtained with isopropanol (a polar solvent) was 56.7 wt% in 3 h using solid:solvent ratio of 0.05 g/mL. Statistics of the model developed demonstrate that solid:solvent ratio had the highest impact on the RSO yield followed by solvent type. Unlike fatty acid content of the RSO, both the quality and yield were affected by isopropanol and n-hexane. In both cases of the solvents, the RSO samples obtained were highly unsaturated (79%) with linoleic and oleic being the main fatty acids. The fatty acid contents were confirmed qualitatively with FT-IR spectra showing regions of double bond stretching and bending vibrations of esters of the triglycerides and trans-unsaturation. The quality of the RSO showed that it is a viable feedstock for oleochemical industries such as biodiesel but needs acid treatment preceding to its transesterification.

Antibacterial activity of Theobroma grandiflorum Schum leaves’ extract against Escherichia coli and Staphylococcus aureus

The present study aimed to obtain ethanol extract of Theobroma Grandiflorum Schum (cupuacu) leaves and evaluate the antibacterial activity of the obtained extract against Escherichia coli and Staphylococcus aureus . The leaves were collected in two different periods of the year and divided into two groups (Group 1 and Group 2), in order to investigate the influence of season of leaves collecting on the effectiveness of the extract. The leaves were cleaned, dried (50 °C/12 h) and grinded for posterior addition of ethanol. Grinded leaves and ethanol mixture was heated (50 °C/1 h) and left to rest (24 h). Solid and liquid parts were separated by filtration. Soxhlet technique was used to recover the ethanol from the separated liquid part. Complete ethanol removal was achieved using an oven, to obtain the dry extract (DLE). The yield obtained was 1.46% and 1.54% for the first and second group, respectively. The extract obtained was diluted in DMSO in distinct proportions (1:3, 1:7, 1:9 (extract: DMSO (w/w)) and tested against Escherichia coli and Staphylococcus aureus , both at a concentration of 10 9 and 10 5 CFU mL -1 , based on the disk diffusion technique. Using the extract from the first group of leaves against 10 9 CFU mL -1 density population, both bacteria were classified as intermediate sensitive. Using the extract obtained from the second group of leaves against 10 5 CFU mL -1 , it was observed that S. aureus kept classified as intermediate sensitive while E. coli was classified as resistant. The results suggest that the DLE can inhibit the growth of tested bacteria. Thus, further studies may be carried out in order to (a) investigate the DLE as a natural product for food preservation and (b) support the search of food industry for alternatives to control such microorganisms.

Biological activity of extracts from guayusa leaves (Ilex guayusa Loes.) obtained by supercritical CO2 and ethanol as cosolvent

To the best of our knowledge, this is the first study to investigate the biological activities and main constituents of extracts from guayusa leaves (Ilex guayusa Loes.) by using supercritical CO2 as solvent and ethanol as cosolvent at different conditions of pressure and temperature and comparing them with Soxhlet technique. The global yield (X0); total phenolic content (TPC), assessed by Folin–Ciocalteu reagent; in vitro antioxidant capacity, by both ABTS•+ and DPPH methods; antimicrobial activity; and the main phytochemicals, based on gas chromatography-mass spectrometry, of the extracts were tested. X0, TPC, and antioxidant capacity were dependent on technique or solvent used and increased when ethanol was used as cosolvent. Antifungal activity was demonstrated against T. rubrum, T. mentagrophytes, M. gypseum and M. canis. The main phytochemicals were caffeine, squalene and α-amyrin. Therefore, known for its caffeine content, guayusa extracts obtained by supercritical fluid extraction showed important nutraceutical ingredients.

Evaluation of Antimicrobial Activities of Sinapis alba and Brassica nigra Leaves Against Selected Microorganisms

Many traditional practitioners in developing countries use the herbaceous plant to treat a different type of microbial infection. Eritrea is one of the developing countries where most of their communities are dependent on herbal medicines for the treatment of infectious disease. However, this malpractice follows incorrect dosage, administration, formulation, frequency and other non-scientific methods with the inevitable negative effect of the practice which makes it inconvenient for the clients who seek treatment. Therefore the current study was carried out to get a scientific evidence of antimicrobial activity of two selected important herbal plants. Active part from leaves of Sinapis alba and Brassica nigra were extracted by continuous hot extraction (Soxhlet technique), and different concentrations were obtained by ethanol, N-hexane, aqueous and DMSO solvents. Microorganisms (Escherichia Coli and Staphylococcus aureus from bacterial strains and Candida Albicans from fungal strain) were selected for testing antimicrobial activity of the plants. Then the extracted solutions were diffused to selected standard organisms inoculated in Muller Hinton Agar using well diffusion technique. Ethanol extracts of S. Alba of 2500mg/ml dissolved in DMSO concentration against E. coli have shown a significant activity with inhibition zones of 30mm. This plant in the same concentration also had a considerable effect against S. aureus and C. Albcaians with a prompting result of 28mm and 25mm zones of inhibition respectively which is greater than the positive control. Moreover, this plant showed almost an equal activity at 1000mg and 250mg which are 20mm and 13mm respectively for C. Albicans, 26mm and 23mm for S. aureus and for E. coli 25mm and17mm. N-hexane extracts of the same plant also showed a remarkable activity at concentrations of 1000mg, 250mg and 50mg, where the zones of inhibition against S. aureus were 18mm, 20mm and 25mm respectively. Ethanol-extract of this plant diluted in ethanol also showed activity at the lowest concentration. Generally, both plants extracted using N-hexane and Ethanol extracts gave a remarkable activity against all the selected micro-organisms.

Enhancement of fatty acids in the oil extracted from the fungus Nigrospora sp. by supercritical CO2 with ethanol as a cosolvent

Fatty acids rich-oil was extracted from freeze-dried fungal biomass of Nigrospora sp. using ultrasound with ethanol as a cosolvent + supercritical CO2. The parameters of temperature (40–80 °C), pressure (15–25 MPa), ultrasound pre-treatment time (15–60 min), and the addition of ethanol (30–70 g/100 g biomass) were evaluated on the oil yield and composition. The best oil yield (3.94 wt.%) and total fatty acids (45.5 g/100 g oil) were obtained at 80 °C and 25 MPa with 50 wt.% ethanol after pre-treating the biomass for 60 min using 132 W of ultrasound power at 40 kHz. The main fatty acids were oleic, palmitic, linoleic, elaidic and palmitoleic acids. The combination of CO2 under supercritical conditions and ultrasound with ethanol as a pre-treatment of the biomass from Nigrospora sp. concentrated up to 4.0 times fatty acids in the oil when compared to the Soxhlet technique, especially the unsaturated ones.

YELLOW PITAYA (HYLOCEREUS MEGALANTHUS) FATTY ACIDS COMPOSITION FROM ECUADORIAN AMAZONIA

Objective: The aim of this study was to determine the composition of methyl esters fatty acids (FAMEs) in yellow pitaya (Hylocereus megalanthus) seeds cultivated in the Palora, Ecuador Amazonian region.Methods: Yellow pitaya oil was obtained from yellow pitaya seeds using the Soxhlet technique. FAMEs identification and quantification were carried out using the gas chromatography (GC) with mass spectrometry (MS) and the database Library NIST14.L to identify the FAMEs present in yellow pitaya oil.Results: Yellow pitaya oil from Ecuador Amazonian region was analyzed by GC-MS, to obtain the five main fatty acids, palmitic acid (11.52%), stearic acid (4.29%), oleic acid (11.09%), vaccenic acid (3.08%), and linoleic acid (69.98%). Omega 6 was the most abundant fatty acid, total content in yellow pitaya seeds oil.Conclusions: Yellow pitaya seeds content a good proportion of polyunsaturated fatty acids (omega 6). For their fatty acid composition, yellow pitaya seeds can be considered as healthy food and can be used in the food industry for different purposes. Regular consumption of yellow pitaya can improve human health.

SOLVENT AND TECHNIQUES EXTRACTION INFLUENCE ON PHYTOCHEMICAL PROFILE AND FREE RADICALS INHIBITION OF PHEONIX DACTYLIFERA L

The main of this investigation was to compare the extraction efficiency of different methods with various solvents from Phoenix dactylifera L extracts. The antioxidant activity and total phenolic compounds of extracts were evaluated using the various method. The highest extraction yield was founded in 70% methanol. Acetone extract exhibited a higher total antioxidant activity, ferric reducing antioxidant power and DPPH radical scavenging activity. The same extract also exhibited the highest phenolic, flavonoid, flavanol and condensed tannins contents. The results revealed that the ultrasonic is most suitable for the extraction of phenolic compounds and thus showing high levels of phenolic content and very high antioxidant capacities relative to the soxhlet and maceration technique. The results propose that Phoenix dactylifera L leaves extract can be considered as a rich source of natural bioactive compounds.

Extraction and Characterization of Crop Oil from Seed Kernels of Feun Kase (Thevetia peruviana)

The seed kernel of Thevetia peruviana is a non-edible material but a potential resource to produce bulb lights in local society of West Timor. The light produced by the seeds makes it a probable feedstock for biodiesel production. In this report, the optimum condition for extraction of the crop oil from the seed kernel of Feun Kase (Thevetia peruviana) using conventional soxhlet technique was studied. The solvent used were chloroform, water, and a chloroform-water binary solvent. The parameters investigated effecting the oil yield involved various solvent polarities, extraction time, and temperature. Each experiment was conducted in 250 mL soxhlet apparatus. The extract was analyzed to examine the physicochemical characteristics including density, kinematic viscosity, acid value, iodine value, saponification value, and water content. The optimum conditions were found after 4.0 h extraction time, extraction temperature of 75 °C and a chloroform-water ratio of 100:0 (polarity index 4.10). The oil extract was found to be 52.43 ± 1.73%. These results revealed that the crop oil from the seed kernel of Feun Kase is a potential feedstock for biodiesel production.

Supercritical extraction of Eugenia involucrata leaves: Influence of operating conditions on yield and α-tocopherol content

This study aimed to evaluate the effects of pressure (150, 175 and 200 bar), temperature (40, 50 and 60 °C) and CO2 flow rate (2, 3 and 4 mL min−1) on the yield of supercritical extraction of Eugenia involucrata leaves. The extracts were characterized for α-tocopherol content and antioxidant activity in comparison to the n-hexane extract obtained by the Soxhlet technique. The greatest yield, 1.73 wt%, was obtained by the highest density and using the highest CO2 flow rate (200 bar, 40 °C and 4 mL min−1). Variation in the experimental conditions resulted in an increase of up to 165% in the α-tocopherol yield, with a possible yield of up to 68.27 mgα-tocopherol 100 gleaves−1. The antioxidant activity determined by the DPPH method showed a high correlation with the content of α-tocopherol, with a maximum of 95.35 μmolTrolox gextract−1. The experimental kinetic curves were satisfactorily described by Sovová mathematical model.

Antibacterial activity of Zingiber officinale roscoe extract as a potential root canal irrigation solution against Enterococcus faecalis

Introduction: This study was aimed to determine and formulate antibacterial activity of Zingiber Officinale Roscoe (Z. officinale) extract against Enterococcus faecalis (E. faecalis) as a potential irrigating solution which is easily available and cost-effective. Methods: Extract of Z. officinale Roscoe was yielded through soxhlet technique. Samples divided into two groups, i.e., the extract of Z. officinale Roscoe and chlorhexidine 2% as a control. The antimicrobial activity was observed using the diffuse agar method. Minimum inhibitory concentration (MIC) was determined by serial microdilution method, and minimum bactericidal concentration (MBC) was determined by cultured samples in blood agar. Data were analysed using ANOVA and Kruskal-Wallis test. Results: Extract of Zingiber officinale Roscoe has antimicrobial activity against E. faecalis with the MBC of 15.625 mg/mL. (p<0,05). Conclusion: Extract of Zingiber officinale Roscoe have shown antibacterial activity against E. faecalis and can be further developed as a potential root canal irrigation solution.

Comparative extraction and simple isolation improvement techniques of active constituents’ momilactone A and B from rice husks of Oryza sativa by HPLC analysis and column chromatography

This paper reports comparative extraction efficiencies and enhancement methods for natural herbicidal (growth inhibitors) compounds, momilactone A and B, respectively from the dried husks of Oryza sativa using different extraction techniques and different solvent systems. Four different extraction techniques viz. percolation, agitation with heat, sonication and soxhlet using five solvent systems as ethyl acetate, acetone, acetonitrile, methanol and methanol:water (8:2) were evaluated. In these studies, it was observed that maximum extract yield was obtained using in methanol and methanol/water mixture as extracting solvent by soxhlet technique although the content of total momilactones A and B was higher in the methanol/water mixture in comparison to other extractions. The successive and simple isolation enrichment technique for momilactones A and B were achieved by solid-matrix partitioning after the treatment of methanolic extract with charcoal and using ethyl acetate as extracting solvent for momilactones A and B. The quantitative analysis of the extraction and enrichment development protocol was validated by a simple, accurate, reproducible RP-HPLC-UV–VIS method using a binary gradient elution comprising of acetonitrile and water (70:30). The separation was achieved on a waters Spherisorb S10 ODS 2 column (250 × 4.6 mm, I.D., 10 µm) that achieved a greater degree of linearity within an overall concentration of extracts and momilactones A and B, 1 mg mL−1 and higher degree of correlation (0.9928 ≤ r2 ≤ 0.9936) for momilactones A and B. So far, comparative extraction of momilactones A and B and HPLC of these compounds has not been reported. Standards of momilactones A (1) and B (2) were isolated along with other two compounds as orizaterpenoid (3) and 7-ketostigmaterol (4) from ethyl acetate extract of rice hulls of O. sativa and checked purity by HPLC-PDA-MS and identification of these isolated compounds (1–4) by complete spectroscopic techniques as IR, 1H NMR, 13C NMR, 2D NMR and HR-MS. The qualitative analysis of momilactone A and B separation technique by thin layer chromatography was also developed.

The Potential of Neural Stem Cell as Vehicle to Deliver Quercus infectoria Extract to Glioma Cell In Vitro

Glioblastoma multiforme (GBM) is the most malignant subtype of brain cancer. However, current clinical treatments for GBM are limited in effectiveness and often impose additional side effects on patients. Here, we developed targeted anti-cancer therapy (TAT) using neural stem cells (NSC) as delivery agent to transport anti-cancer compounds directly to GBM in vitro. Anti-cancer active compounds: Tannic acid (TA) and gallic acid (GA) were extracted from local medicinal plant - Quercus infectoria (QI) using soxhlet technique with 100% methanol (QI-100%) or 70% methanol (QI-70%) solvent. Concentration of TA and GA measured using HPLC were 72.56 and 43.66 μg/mL in QI-100%, while in QI-70%, the concentrations were 72.41 and 43.31 μg/mL, respectively. Cytotoxicity effects of QI-100% and QI-70% on human GBM cell line (DBTRG-05MG), human NSC line (H9-hNSC) and human normal brain glial cell line (SVG-p12) (as negative control) were determined using MTT assay. Both QI-100% and QI-70% showed anti-proliferative properties against DBTRG-05MG at IC50, but not on H9-hNSC and SVG-p12. Taken together, data indicated that both QI extracts contained TA and GA which exhibit anti-proliferative effect specifically on cancerous cells only. Next, QI-treated H9-hNSC was seeded in a modified Boyden chamber for 12 h to investigate its migration capacity towards DBTRG-05MG. The result showed that H9-hNSC migrated towards DBTRG-05MG with 4-folds higher capacity compared to control. In addition, the migration of QI-100% treated H9-hNSC successfully reduced the number of DBTRG-05MG, indicating the anti-GBM potential of these cells after migration. In conclusion, NSC could be a specific anti-cancer compound delivery agent for GBM, reducing unwanted side effects on patients.