This study was aimed to compare the efficiency of silymarin extraction from Silybi mariani fructus using different extraction methods. Maceration, percolation, extraction in a water bath, and ultrasonic-assisted extraction have been performed with 60% ethanol. Extracts were analyzed using UV/Vis and HPLC techniques. Our results showed that the highest concentration of silymarin was detected in samples extracted in a water bath with boiling solvent during 30 and 60 minutes (2.33 and 1.95 %, respectively). Since extraction in a water bath is efficient and reduces a lot of time and solvent consumption, this method could present potential replacement for Pharmacopoeia-recommended Soxhlet extraction procedure. .