Southern Mozambique Research Articles

Impact of rotavirus vaccination on diarrheal hospitalizations in children younger than 5 years of age in a rural southern Mozambique

BackgroundRotavirus vaccine(Rotarix®) was introduced in Mozambique through its Expanded Program of Immunization in September 2015. We assessed the impact of rotavirus vaccination on childhood gastroenteritis-associated hospitalizations post-vaccine introduction in a high HIV prevalence rural setting of southern Mozambique. MethodsWe reviewed and compared the trend of hospitalizations (prevalence) and incidence rates of acute gastroenteritis (AGE), and rotavirus associated-diarrhea (laboratory confirmed rotavirus) in pre- (January 2008–August 2015) and post-rotavirus vaccine introduction periods (September 2015–December 2020), among children <5 years of age admitted to Manhiça District Hospital. ResultsFrom January 2008 to December 2020, rotavirus vaccination was found to contribute to the decline of the prevalence of AGE from 19% (95% CI: 18.14–20.44) prior to the vaccine introduction to 10% (95% CI: 8.89–11.48) in the post-introduction period, preventing 40% (95 % IE: 38–42) and 84% (95 % IE: 80–87) of the expected AGE and laboratory confirmed rotavirus cases, respectively, among infants. Similarly, the overall incidence of rotavirus was 11.8-fold lower in the post-vaccine introduction period (0.4/1000 child-years-at-risk [CYAR]; 95% CI: 0.3–0.6) compared with the pre-vaccination period (4.7/1000 CYAR; 95% CI: 4.2–5.1) with the highest reduction being observed among infants (16.8-fold lower from the 15.1/1000 CYAR in the pre-vaccine to 0.9/1000 CYAR in the post-vaccine eras). ConclusionsWe documented a significant reduction in all-cause diarrhea hospitalizations and rotavirus positivity after vaccine introduction demonstrating the beneficial impact of rotavirus vaccination in a highly vulnerable population.

Caregivers’ psychosocial assessment for identifying HIV-infected infants at risk of poor treatment adherence: an exploratory study in southern Mozambique

ABSTRACT Psychosocial support (PSS) to caregivers of HIV-infected infants on antiretroviral treatment (ART) is crucial to ensure ART adherence and sustained long-term viral suppression in children. A specific approach including tools to monitor and understand adherence behavior and risk factors that prevent optimal treatment compliance are urgently needed. This qualitative exploratory study, conducted in southern Mozambique, monitored the infants’ viral response trajectories during 18 months follow-up, as a measure of adherence, reviewed the caregiver’s PSS session notes and the answers to a study questionnaire, to analyze whether the standard PSS checklist applied to infants’ caregivers can identify barriers influencing their adherence. Only 9 of 31 infants had sustained virologic response. Reported factors affecting adherence were: difficulties in drugs administration, shared responsibility to administer treatment; disclosure of child’s HIV status to family members but lack of engagement; mother’s ART interruption and poor viral response. In conclusion, we found that the standard PSS approach alone, applied to caregivers, was lacking focus on many relevant matters that were identified by the study questionnaire. A comprehensive patient-centered PSS package of care, including an adherence risk factor monitoring tool, tailored to caregivers and their children must be developed.

The effect of landscape fires on child hospital visits and admissions: a time-series study in southern Mozambique

The effect of landscape fires on child hospital visits and admissions: a time-series study in southern Mozambique

The mosquito vectors that sustained malaria transmission during the Magude project despite the combined deployment of indoor residual spraying, insecticide-treated nets and mass-drug administration.

The "Magude project" aimed but failed to interrupt local malaria transmission in Magude district, southern Mozambique, by using a comprehensive package of interventions, including indoor residual spraying (IRS), pyrethroid-only long-lasting insecticide treated nets (LLINs) and mass-drug administration (MDA). Here we present detailed information on the vector species that sustained malaria transmission, their association with malaria incidence and behaviors, and their amenability to the implemented control interventions. Mosquitoes were collected monthly between May 2015 and October 2017 in six sentinel sites in Magude district, using CDC light traps both indoors and outdoors. Anopheles arabiensis was the main vector during the project, while An. funestus s.s., An. merus, An. parensis and An. squamosus likely played a secondary role. The latter two species have never previously been found positive for Plasmodium falciparum in southern Mozambique. The intervention package successfully reduced vector sporozoite rates in all species throughout the project. IRS was effective in controlling An. funestus s.s. and An. parensis, which virtually disappeared after its first implementation, but less effective at controlling An. arabiensis. Despite suboptimal use, LLINs likely provided significant protection against An. arabiensis and An. merus that sought their host largely indoors when people where in bed. Adding IRS on top of LLINs and MDA likely added value to the control of malaria vectors during the Magude project. Future malaria elimination attempts in the area could benefit from i) increasing the use of LLINs, ii) using longer-lasting IRS products to counteract the increase in vector densities observed towards the end of the high transmission season, and iii) a higher coverage with MDA to reduce the likelihood of human infection. However, additional interventions targeting vectors that survive IRS and LLINs by biting outdoors or indoors before people go to bed, will be likely needed to achieve local malaria elimination.

Costs and cost-effectiveness of HIV counselling and testing modalities in Southern Mozambique

ObjectiveDespite the high HIV associated burden, Mozambique lacks data on HIV counselling and testing (HCT) costs. To help guide national HIV/AIDS programs, we estimated the cost per test for voluntary counselling and testing (VCT) from the patient’s perspective and the costs per person tested and per HIV-positive individual linked to care to the healthcare provider for VCT, provider-initiated counselling and testing (PICT) and home-based testing (HBT). We also assessed the cost-effectiveness of these strategies for linking patients to care.MethodsData from a cohort study conducted in the Manhiça District were used to derive costs and linkage-to-care outcomes of the three HCT strategies. A decision tree was used to model HCT costs according to the likelihood of HCT linking individuals to care and to obtain the incremental cost-effectiveness ratios (ICERs) of PICT and HBT with VCT as the comparator. Sensitivity analyses were performed to assess robustness of base-case findings.FindingsBased on costs and valuations in 2015, average and median VCT costs to the patient per individual tested were US$1.34 and US$1.08, respectively. Costs per individual tested were greatest for HBT (US$11.07), followed by VCT (US$7.79), and PICT (US$7.14). The costs per HIV-positive individual linked to care followed a similar trend. PICT was not cost-effective in comparison with VCT at a willingness-to-accept threshold of US$4.53, but only marginally given a corresponding base-case ICER of US$4.15, while HBT was dominated, with higher costs and lower impact than VCT. Base-case results for the comparison between PICT and VCT presented great uncertainty, whereas findings for HBT were robust.ConclusionPICT and VCT are likely equally cost-effective in Manhiça. We recommend that VCT be offered as the predominant HCT strategy in Mozambique, but expansion of PICT could be considered in limited-resource areas. HBT without facilitated linkage or reduced costs is unlikely to be cost-effective.

DNA barcoding to assess species identification in museum samples of Amphiliidae and natural samples of Cichlidae from Southern Mozambique

The biodiversity protection and monitorning is one of main goals of natural history musems worldwide. Conservation issues are particularly important for freshwater fish which are one of the most threatened taxa for the consequences of climate change and human activies. In Mozambique freshwater rivers are poorly explored and the impact of aquaculture and human activities on local biodiversity in almost unknown. Here we propose the barcoding analysis of cytochrome c oxidase I (COI) mitochrondrial DNA of 41 frehswater fishes catched in four rivers of southern Mozambique and 53 from a museum collection. As evidence of previous knowledge gaps, barcoding results revealed twenty new haplotypes described for the first time in the taxa Cichlidae and Amphilidae. From a methodological point of view, the barcoding approach demonstrated a critical point connected to the requested 650 bp length of amplified sequences. In fact, high weight genomic DNA is unattainable from museum samples and also in wildlife samples collected in pristine rivers. For this reason we furtherly tested the efficiency of DNA mini-barcoding analysis for 53 fish from a museum collection. The Mini-barcode method retrieved 56.6% of sequences successfully analyzed versus 3% of barcoding. The high performance of this thecniques is discussed in relation to biodiversity monitoring and to fill the taxonomy gaps in museum collections.

INaturalist is useful at enhancing biodiversity studies as evident from southern African freshwater crabs (Decapoda: Brachyura: Potamonautidae)

Abstract The value of the citizen science platform iNaturalist was explored using photographic observations of southern African freshwater crabs (Potamonautidae Bott, 1970, among two genera Potamonautes sensu stricto MacLeay, 1838 and MartimonautesCumberlidge &amp; Daniels, 2022) in combination with specimen data deposited in the South African Museum (Iziko Museums of Cape Town, South Africa). Species identification of photographic observations were assessed, and the identification of taxa corrected where necessary. From these records, the habitat type and distribution of the species were noted. The area of occupancy (AOO) and the extent of occurrence (EOO) were calculated where range extensions for species were observed. The results demonstrate that riverine/mountain stream freshwater crabs are over-represented in their frequency but species occurring in swamps/forests were under-represented. Using iNaturalist spatial data for South Africa we can identify several unsampled gaps in the country which included the Free State and North West, Gauteng and the Northern Cape, followed by Limpopo, Mpumalanga, and the Eastern Cape provinces. The Western Cape and KwaZulu-Natal had the highest number of freshwater crab records on iNaturalist. The remaining southern African countries, Angola, Botswana, Eswatini, Lesotho, Malawi, Mozambique, Namibia, and Zimbabwe, have few iNaturalist records. Range extensions were observed in four South African species based on iNaturalist records (P. clarusGouws, Stewart &amp; Coke, 2000, P. flavusjoDaniels, Phiri, &amp; Bayliss, 2014, P. isimangaliso Peer &amp; Gouws, 2015, and P. mariepskoppieDaniels, Barnes, Marais &amp; Gouws, 2021) with increases in AOO and EOO. We also observed a new undescribed freshwater crab on iNaturalist and corroborated this observation by sequencing these specimens for the cytochrome oxidase one subunit (COI) locus. Photographic quality is critical for taxonomic inference. Citizen science ­platforms such as iNaturalist can be invaluable for the discovery and documentation of biodiversity and provide new spatial data for species distributions that in turn can aid conservation tools.

Resurrection of Lethrinus scoparius Gilchrist amp; Thompson, 1908 (Perciformes: Lethrinidae), from South African waters.

Lethrinus nebulosus (Forsskl, 1775) is distributed throughout the Indian Ocean, Indonesia and the West-central Pacific. Given recent evidence suggesting two cryptic species of L. nebulosus in the southwestern Indian Ocean, we implemented a multispecies coalescent approach using nuclear and mitochondrial DNA loci and conducted Bayesian tests of species delimitation. One population extends from southern Mozambique to Kenya, and the other from northern KwaZulu-Natal (South Africa) southwards, with a zone of overlap between them. This study confirms that the South African population can be identified as a separate species, previously identified as Lethrinus scoparius Gilchrist Thompson, 1908, and can be distinguished from L. nebulosus by live and fresh colouration and with genetic data, but not consistently by morphometric or meristic parameters, other than in preorbital length cf. head length. Lethrinus scoparius is resurrected and redescribed herein.

Prompt HIV diagnosis and antiretroviral treatment in postpartum women is crucial for prevention of mother to child transmission during breastfeeding: Survey results in a high HIV prevalence community in southern Mozambique after the implementation of Option B.

World Health Organization recommends promoting breastfeeding without restricting its duration among HIV-positive women on lifelong antiretroviral treatment (ART). There is little data on breastfeeding duration and mother to child transmission (MTCT) beyond 24 months. We compared the duration of breastfeeding in HIV-exposed and HIV-unexposed children and we identified factors associated with postpartum-MTCT in a semi-rural population of Mozambique. This cross-sectional assessment was conducted from October-2017 to April-2018. Mothers who had given birth within the previous 48-months in the Manhiça district were randomly selected to be surveyed and to receive an HIV-test along with their children. Postpartum MTCT was defined as children with an initial HIV positive result beyond 6 weeks of life who initiated breastfeeding if they had a first negative PCR result during the first 6 weeks of life or whose mother had an estimated date of infection after the child's birth. Cumulative incidence accounting for right-censoring was used to compare breastfeeding duration in HIV-exposed and unexposed children. Fine-Gray regression was used to assess factors associated with postpartum-MTCT. Among the 5000 mother-child pairs selected, 69.7% (3486/5000) were located and enrolled. Among those, 27.7% (967/3486) children were HIV-exposed, 62.2% (2169/3486) were HIV-unexposed and for 10.0% (350/3486) HIV-exposure was unknown. Median duration of breastfeeding was 13.0 (95%CI:12.0-14.0) and 20.0 (95%CI:19.0-20.0) months among HIV-exposed and HIV-unexposed children, respectively (p<0.001). Of the 967 HIV-exposed children, 5.3% (51/967) were HIV-positive at the time of the survey. We estimated that 27.5% (14/51) of the MTCT occurred during pregnancy and delivery, 49.0% (2551) postpartum-MTCT and the period of MTCT remained unknown for 23.5% (12/51) of children. In multivariable analysis, mothers' ART initiation after the date of childbirth was associated (aSHR:9.39 [95%CI:1.75-50.31], p = 0.001), however breastfeeding duration was not associated with postpartum-MTCT (aSHR:0.99 [95%CI:0.96-1.03], p = 0.707). The risk for postpartum MTCT was nearly tenfold higher in women newly diagnosed and/or initiating ART postpartum. This highlights the importance of sustained HIV screening and prompt ART initiation in postpartum women in Sub-Saharan African countries. Under conditions where HIV-exposed infants born to mothers on ART receive adequate PMTCT, extending breastfeeding duration may be recommended.

Evidence of Závora Bay as a critical site for reef manta rays, Mobula alfredi, in southern Mozambique.

The largest known reef manta ray (Mobula alfredi) population in Africa has been monitored for more than 20 years at several locations on the coast of the Inhambane Province in southern Mozambique. Nonetheless, before this study, little had been reported on the population dynamics of M. alfredi from Závora, a remote bay in the region. Photographic mark‐recapture was used to investigate the size and structure of M. alfredi that aggregate at “Red Sands,” a reef cleaning station in Závora Bay. An 11 year photographic data set was used to identify 583 M. alfredi individuals between 2010 and 2021. More than half of M. alfredi individuals were resighted at least once, with most encounters (up to 18 for one individual) occurring during the peak sighting period in July–November each year. An even sex ratio was observed, 44% females and 50% males, with no significant difference in resightings between the sexes. Pollock's robust design population models were used to estimate annual abundance, emigration, annual apparent survival and capture probability at Red Sands from July to November over a 6 year period (2016–2021). Abundance estimates varied year to year, ranging from 35 (95% c.i. [30, 45]) up to 233 (95% c.i. [224, 249]) M. alfredi individuals. Given the seasonal affinity of M. alfredi observed at Red Sands, this study highlights the importance of understanding fine‐scale site use within the larger home range of this population to develop local management strategies.

Single-Nucleotide Polymorphisms in the Beta-Tubulin Gene and Its Relationship with Treatment Response to Albendazole in Human Soil-Transmitted Helminths in Southern Mozambique.

ABSTRACT.Soil-transmitted helminth (STH) cornerstone control strategy is mass drug administration (MDA) with benzimidazoles. However, MDA might contribute to selection pressure for anthelmintic resistance, as occurred in livestock. The aim of this study is to evaluate the treatment response to albendazole and the relationship with the presence of putative benzimidazole resistance single-nucleotide polymorphisms (SNPs) in the β-tubulin gene of STH in Southern Mozambique. After screening 819 participants, we conducted a cohort study with 184 participants infected with STH in Manhiça district, Southern Mozambique. A pretreatment and a posttreatment stool samples were collected and the STH infection was identified by duplicate Kato-Katz and quantitative polymerase chain reaction (qPCR). Cure rate and egg reduction rates were calculated. Putative benzimidazole resistance SNPs (F167Y, F200T, and E198A) in Trichuris trichiura and Necator americanus were assessed by pyrosequencing. Cure rates by duplicate Kato-Katz and by qPCR were 95.8% and 93.6% for Ascaris lumbricoides, 28% and 7.8% for T. trichiura, and 88.9% and 56.7% for N. americanus. Egg reduction rate by duplicate Kato-Katz was 85.4% for A. lumbricoides, 34.9% for T. trichiura, and 40.5% for N. americanus. Putative benzimidazole resistance SNPs in the β-tubulin gene were detected in T. trichiura (23%) and N. americanus (21%) infected participants at pretreatment. No statistical difference was observed between pretreatment and posttreatment frequencies for none of the SNPs. Although treatment response to albendazole was low, particularly in T. trichiura, the putative benzimidazole resistance SNPs were not higher after treatment in the population studied. New insights are needed for a better understanding and monitoring of human anthelmintic resistance.

Coping Strategies for Household Food Insecurity, and Perceived Health in an Urban Community in Southern Mozambique: A Qualitative Study

In low- and middle-income countries, food insecurity (FI) is a living reality for many households, particularly among the most vulnerable groups. The burden of household FI in Mozambique and how FI and coping strategies relate to perceived health are unknown. This study investigated the lived experiences and coping strategies of food-insecure households, along with their perceived health. Altogether, 16 in-depth interviews were performed, audio-recorded, and transcribed verbatim. A qualitative content analysis was carried out and five themes emerged: lived experiences of FI, coping strategies used in situations of FI, food choices, climate change and food security, and FI and perceived health. A wide range of lived experiences and coping strategies were reported, including cooking whatever is available, skipping meals, receiving money or food from friends and relatives, eating unsafe and low-quality foods, taking on additional work, cooking least-preferred foods, and having a monotonous and less-nutritious diet. Furthermore, the participants reported emotional distress, anxiety and depression, substance use, and other negative health outcomes. Some had diagnoses of hypertension, diabetes or HIV/AIDS. The findings suggest the need for employment creation and women’s empowerment, as well as the implementation of appropriate policies and programmes to alleviate household FI.

To spray or target mosquitoes another way: focused entomological intelligence guides the implementation of indoor residual spraying in southern Mozambique

BackgroundTo eliminate malaria in southern Mozambique, the National Malaria Control Programme and its partners are scaling up indoor residual spraying (IRS) activities in two provinces, Gaza and Inhambane. An entomological surveillance planning tool (ESPT) was used to answer the programmatic question of whether IRS would be effective in target geographies, given limited information on local vector bionomics.MethodsEntomological intelligence was collected in six sentinel sites at the end of the rainy season (April–May 2018) and the beginning of the dry season (June–July 2018). The primary objective was to provide an ‘entomological snapshot’ by collecting question-based, timely and high-quality data within one single week in each location. Host-seeking behaviour (both indoors and outdoors) was monitored by human-baited tent traps. Indoor resting behaviour was quantified by pyrethrum spray catches and window exit traps.ResultsFive different species or species groups were identified: Anopheles funestus sensu lato (s.l.) (66.0%), Anopheles gambiae s.l. (14.0%), Anopheles pharoensis (1.4%), Anopheles tenebrosus (14.1%) and Anopheles ziemanni (4.5%). Anopheles funestus sensu stricto (s.s.) was the major vector among its sibling species, and 1.9% were positive for Plasmodium falciparum infections. Anopheles arabiensis was the most abundant vector species within the An. gambiae complex, but none tested positive for P. falciparum infections. Some An. tenebrosus were positive for P. falciparum (1.3%). When evaluating behaviours that impact IRS efficacy, i.e. endophily, the known primary vector An. funestus s.s., was found to rest indoors—demonstrating at least part of its population will be impacted by the intervention if insecticides are selected to which this vector is susceptible. However, other vector species, including An. gambiae s.l., An. tenebrosus, An. pharoensis and An. ziemanni, showed exophilic and exophagic behaviours in several of the districts surveilled.ConclusionThe targeted approach to entomological surveillance was successful in collecting question-based entomological intelligence to inform decision-making about the use of IRS in specific districts. Endophilic An. funestus s.s. was documented as being the most prevalent and primary malaria vector suggesting that IRS can reduce malaria transmission, but the presence of other vector species both indoors and outdoors suggests that alternative vector control interventions that target these gaps in protection may increase the impact of vector control in southern Mozambique.

Fine-grain population structure and transmission patterns of Mycobacterium tuberculosis in southern Mozambique, a high TB/HIV burden area

Genomic studies of the Mycobacterium tuberculosis complex (MTBC) might shed light on the dynamics of its transmission, especially in high-burden settings, where recent outbreaks are embedded in the complex natural history of the disease. To this end, we conducted a 1 year prospective surveillance-based study in Mozambique. We applied whole-genome sequencing (WGS) to 295 positive cultures. We fully characterized MTBC isolates by phylogenetics and dating evaluation, and carried out a molecular epidemiology analysis to investigate further associations with pre-defined transmission risk factors. The majority of strains (49.5%, 136/275) belonged to lineage (L) 4; 57.8 % of them (159/275) were in genomic transmission clusters (cut-off 5 SNPs), and a strikingly high proportion (45.5%) shared an identical genotype (0 SNP pairwise distance). We found two ‘likely endemic’ clades, comprising 67 strains, belonging to L1.2, which dated back to the late 19th century and were associated with recent spread among people living with human immunodeficiency virus (PLHIV). We describe for the first time the population structure of MTBC in our region, a high tuberculosis (TB)/HIV burden area. Clustering analysis revealed an unforeseen pattern of spread and high rates of progression to active TB, suggesting weaknesses in TB control activities. The long-term presence of local strains in Mozambique, which were responsible for large transmission among HIV/TB-coinfected patients, calls into question the role of HIV in TB transmission.

IMPACTS OF ACCESS TO RURAL CREDIT AS A MEANS OF ACQUISITION OF IMPROVED PRODUCTION TECHNOLOGIES IN THE SOUTH AREA OF MOZAMBIQUE: AN ANALYSIS BASED ON THE 2012-2020 AGRICULTURAL CENSES.

The main objective of this work is to address the impacts of access to rural credit on the acquisition of improved agricultural production technologies by small and medium-sized farms in the provinces of Maputo, Gaza and Inhambane in southern Mozambique. The analysis was based on statistical data from the 2012-2014 statistical year book and the 2020 integrated agricultural survey of the Ministry of Agriculture and Food Security of Mozambique. In order to achieve this objective, the different data from the aforementioned instruments were compiled, organized and cross-referenced for the variables access to agricultural credit and different agricultural production technologies, namely pesticides, fertilizers and irrigation. From the results it was found that in the period 2012-2020 the levels of exclusion and access of small and medium rural producers remain steady, as since 2012 less than 10% of these producers had access to these services. By the way, access to improved agricultural technologies by these producers also remains insignificant. Thus, it is essential to increase public policies that encourage the financial sector in the allocation of rural credit to improve the levels of technological application in rural production in these 3 provinces.

Short-Term Effects of Tillage Systems, Fertilization, and Cropping Patterns on Soil Chemical Properties and Maize Yields in a Loamy Sand Soil in Southern Mozambique

Sub-Saharan Africa (SSA) agriculture is characterized by dependence on erratic rainfall, inadequate conservation practices, and a decline in soil fertility resulting in low crop productivity. Therefore, conservation agriculture (CA) has been proposed as an alternative to improve soil fertility and productivity. Hence the aim was to investigate the effects of tillage systems, fertilization, and cropping patterns on selected soil chemical properties (pH, organic carbon, total nitrogen, extractable phosphorus, exchangeable cations, and cation exchange capacity) and identify which cropping pattern maximizes stover and grain maize–legume productivity and land use. A two-year (2016/17–2017/18) field experiment in a loamy sand soil was conducted at Nhacoongo Research Station, southern Mozambique. Two tillage systems (conservation (CA) and conventional tillage (CT)), two fertilization treatments (fertilized and unfertilized), and seven cropping patterns (four sole crops and three maize–legume intercrops) were evaluated in a randomized complete block design with split–split plot arrangement and replicated four times. CA practices resulted in significantly higher soil chemical properties and increased stover and grain yields as compared to CT practices, but fertilization demonstrated insignificant effects on soil chemical properties and significant influences on stover and grain yield of maize and legumes. Cropping patterns induced no significant effect on soil chemical properties and either stover or grain yield. Estimated indices like land equivalent ratio (1.18–2.67) and competitive ratio index (0.01–1.72) confirmed the advantage of intercropping against sole cropping. This is largely supported by the estimated values of aggressivity and relative crowding coefficient. Smallholder farmers can therefore benefit by adopting CA.

A 334-year coral record of surface temperature and salinity variability in the greater Agulhas Current region

Abstract. The Agulhas Current (AC) off the southern tip of Africa is one of the strongest western boundary currents and a crucial choke point of inter-ocean heat and salt exchange between the Indian Ocean and the southern Atlantic Ocean. However, large uncertainties remain concerning the sea surface temperature (SST) and salinity (SSS) variability in the AC region and their driving mechanisms over longer timescales, due to only short observational datasets being available and the highly dynamic nature of the region. Here, we present an annual coral skeletal Sr/Ca composite record paired with an established composite oxygen isotope record from Ifaty and Tulear reefs in southwestern Madagascar to obtain a 334-year (1661–1995) reconstruction of δ18Oseawater changes related to surface salinity variability in the wider Agulhas Current region. Our new annual δ18Oseawater composite record from Ifaty traces surface salinity of the southern Mozambique Channel and AC core region from the SODA reanalysis between 1958 and 1995. δ18Oseawater appears to be mainly driven by large-scale wind forcing in the southern Indian Ocean on interannual to decadal timescales. The δ18Oseawater and SST at Ifaty show characteristic interannual variability of between 2 and 4 years and interdecadal variability of 8 to 16 years, coherent with El Niño–Southern Oscillation (ENSO) records. Lagged correlations with the multivariate ENSO index reveals a 1–2-year lag of δ18Oseawater and salinity at Ifaty and the AC region, suggesting that propagation of anomalies by ocean Rossby waves may contribute to salinity changes in the wider southwestern Indian Ocean. The δ18Oseawater and SST reconstructions at Ifaty reveal the highest interannual variability during the Little Ice Age, especially around 1700 CE, which is in agreement with other Indo-Pacific coral studies. Our study demonstrates the huge potential to unlock past interannual and decadal changes in surface ocean hydrology and ocean transport dynamics from coral δ18Oseawater beyond the short instrumental record.

Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique.

BackgroundSoil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure.MethodologyWe evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference.Principal findingsIgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed.ConclusionsWe provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.

The contribution of wild palms to the livelihoods and diversification of rural households in southern Mozambique

In most low-income countries, rural households combine a range of economic activities to generate income, achieve food and nutritional security and fulfill other livelihood objectives, including: small-scale farming, fishing, hunting, livestock and timber production, and non-timber forest products gathering, as well as off-farm activities such as wage labour and informal trading. This work examines the role of diversification, including palm products income, on rural livelihoods in the Zitundo area, southern Mozambique. Seven livelihood strategies were identified, with every strategy combining cash income from several sources. Most households adopt a wage-based strategy to some degree, although this strategy shows low potential for reducing poverty in the area. Households adopting a palm-based strategy show between 23% and 60% lower poverty incidence than those adopting alternatives strategies. Yet, a palm-based livelihood strategy was adopted by only 11% of households. Traders of palm products earn approximately double the annual income and per capita income than non-traders. Village of residency and some household socio-economic and demographic characteristics correlate with a households' choice of livelihood strategy and palm income dependency. The adherence of many households to a less remunerative wage strategy indicates other factors also influence the choice of livelihood strategies. The study shows that future development plans and policy interventions to mitigate poverty in the area should go beyond the usual agricultural sector and recognize the role of non-timber forest products, especially palm products, for people's livelihoods and poverty alleviation.

The status and distribution of three species of Sternula terns on the eastern coast of Africa and in the western Indian Ocean, with two species new for Mozambique

The status of three Sternula terns in southern and eastern Africa and the Indian Ocean is updated based on observations in Mozambique during October 2009–August 2021. Damara Tern S. balaenarum and Saunders's Tern S. saundersi, both new to Mozambique, were found at the San Sebastian Peninsula in August 2018 and have been recorded regularly since. Damara Tern arrived in May, numbers were lower June–July, with a peak of 100+ in August–October. Breeding plumage was assumed by late October and all departed in early November; those aged were all adults. Observations of ringed birds suggested they originated from colonies in South Africa. Numbers of Damara Tern reached the Key Biodiversity Area designation threshold population for the site in 2020. These are the first records of large numbers of Damara Tern in the Indian Ocean, whilst timings suggest the species is partly transient at the site and may also occur further north. Saunders's Tern was recorded at San Sebastian in all months except March and April, with 5–80 regularly present, and peak numbers perhaps in October–December. Birds were observed in a range of plumages in all months, from non-breeding, transitional to full breeding. Courtship feeding was observed in September–November suggesting at least some attempt to breed locally, although disturbance is high. Birds in non-breeding plumage may stem from Northern Hemisphere breeding localities. Little Tern S. albifrons was a numerous migrant in southern Mozambique in October–May, peaking in April when large numbers were observed during pelagic trips to the continental shelf edge, and at onshore roost sites including San Sebastian. Smaller numbers fed in coastal lagoons and freshwater lakes. Three Little Terns had been colour-ringed in Israel.