Chilli is one of the widely cultivated commercial vegetable crops in India. Chilli leaf curl is the most devasting disease and causes a yield loss of 100 per cent in Chilli. Fourteen begomovirus species cause chilli leaf curl disease (ChiLCD). An extensive survey was carried out in 2023 to determine the prevalence of the leaf curl disease of Chilli caused by begomovirus in major chilli-growing areas of the Southern Districts of Tamil Nadu. During the survey, 21 isolates of begomovirus infecting Chilli were collected. Among the 21 isolates, the isolate collected from Kalakudi village (BV-KI) exhibited severe symptoms.DNA extracted from 21 isolates of begomovirus were subjected to PCR by using Rojas universal primers, Chilli leaf curl virus (ChiLCV) Coat protein primers and beta satellite virus primers and obtained 1380 bp, 1100 bp and 1400 bp amplicons of top and coat protein region of DNA-A of begomovirus, Chilli leaf curl virus coat protein region and beta satellite virus associated with ChiLCV respectively. The 1380 bp, 1100 bp and 1400 bp amplicons obtained from the BV-KI isolate were sequenced and submitted to the NCBI. The sequences were blast analyzed, and it showed that the nucleotide sequence of the top and coat protein region of DNA-A of begomovirus and CP gene of ChiLCV BV-KI isolate was found to have 90.61 and 99.78% similarity with Bhavanisagar (NC 055130.1) isolate respectively. The nucleotide sequence of the beta satellite virus of BV-KI isolate was found to have 99.93% similarity with the Chilli leaf curl beta satellite virus Meerut (MH355642.1) isolate.
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