Southeastern Madagascar Research Articles

Towards elimination of lymphatic filariasis in southeastern Madagascar: Successes and challenges for interrupting transmission.

IntroductionA global strategy of mass drug administration (MDA) has greatly reduced the burden of lymphatic filariasis (LF) in endemic countries. In Madagascar, the National Programme to eliminate LF has scaled-up annual MDA of albendazole and diethylcarbamazine across the country in the last decade, but its impact on LF transmission has never been reported. The objective of this study was to evaluate progress towards LF elimination in southeastern Madagascar.MethodsThree different surveys were carried out in parallel in four health districts of the Vatovavy Fitovinany region in 2016: i) a school-based transmission assessment survey (TAS) in the districts of Manakara Atsimo, Mananjary, and Vohipeno (following a successful pre-TAS in 2013); ii) a district-representative community prevalence survey in Ifanadiana district; and iii) a community prevalence survey in sentinel and spot-check sites of these four districts. LF infection was assessed using the Alere Filariasis Test Strips, which detect circulating filarial antigens (CFA) of adult worms. A brief knowledge, attitudes and practices questionnaire was included in the community surveys.Principal findingsNone of the 1,825 children sampled in the TAS, and only one in 1,306 children from sentinel and spot-check sites, tested positive to CFA. However, CFA prevalence rate in individuals older than 15 years was still high in two of these three districts, at 3.5 and 9.7% in Mananjary and Vohipeno, respectively. Overall CFA prevalence in sentinel and spot-check sites of these three districts was 2.80% (N = 2,707), but only two individuals had detectable levels of microfilaraemia (0.06%). Prevalence rate estimates for Ifanadiana were substantially higher in the district-representative survey (15.8%; N = 545) than in sentinel and spot-check sites (0.8%; N = 618). Only 51.2% of individuals surveyed in these four districts reported taking MDA in the last year, and 42.2% reported knowing about LF.ConclusionsAlthough TAS results suggest that MDA can be stopped in three districts of southeastern Madagascar, the adult population still presents high CFA prevalence levels. This discordance raises important questions about the TAS procedures and the interpretation of their results.

The use of natural resources to improve household income, health, and nutrition within the forests of Kianjavato, Madagascar

Understanding natural resource extraction in Madagascar is key to developing wider conservation and management strategies that ensure the continued delivery of essential ecosystem services, and the development of health and economic strategies to meet the demands of a growing human population. In the Kianjavato lowland rainforest of southeastern Madagascar, 78% of lemur species, 83% of native carnivoran species, and 67% of frugivorous bat species are threatened with extinction. All three of these groups of mammals are commonly hunted for food in other regions of Madagascar, yet we knew little about current levels of hunting or whether the use of forest resources significantly affects human welfare in this region. We used health assessments of 1 267 Kianjavato residents and semi-structured interviews of members of 336 households in 1 7 communities in Kianjavato to investigate human-environmental interactions. We found high prevalence of child and teenage malnutrition. More than half of the population under 20 years old was stunted, more than half was underweight, and more than one fifth was wasted. Further, one in six residents were anemic. We found that Kianjavato’s forests provided essential ecosystem services for its rural communities. As Kianjavato’s forests are altered to meet the needs of a growing human population, these direct-use ecosystem services (such as the use of wild animals for food or plants for medicine) are unable to similarly expand to meet the basic needs of the families living in these forests. A worrying proportion of the Kianjavato population depends on forests to meet their healthcare, nutritional, and economic needs, yet they may be failing to do so. All surveyed households (1 00%) depended on forests to meet their healthcare needs and 40% of the regional economy involved the extraction of finite forest resources (i.e., precious stones). Most households consumed very little wildlife (two animals per household per year) and the great majority (83%) of the forest animals they ate were not threatened with extinction. Forty percent of wildlife were caught illegally. Although hunting is likely not imperiling local wildlife at present, 1 6% of the Kianjavato population hunts wildlife and 20% are malnourished. The ecosystem services of Kianjavato’s forests may currently be insufficient to meet the needs of residents. Therefore, targeted efforts to increase local access to the healthcare system and to improve household nutrition and economy by improving the production and stability of local agricultural products may increase the long-term sustainability of wildlife hunting, household incomes, and regional food security. Efforts such as these that can expand with the growing population to meet future needs may help secure the sustainable use of essential ecosystem services for the long term, improving the health of local people and maintaining the integrity of the forests in which they live.

Ectoparasitism in Black-and-White Ruffed Lemurs ( Varecia variegata) in Southeastern Madagascar.

We documented ectoparasites found on wild black-and-white ruffed lemurs ( Varecia variegata) in the southeastern rain forests of Madagascar and describe trends in parasitism. In this study, 235 mesostigmatid mites (1 male, 87 females, 147 nymphs) identified as Liponyssella sp., in the acarine family Macronyssidae, were collected during 87% (34/39) of lemur examinations (mean number/host=7.9). The only other ectoparasite collected was the louse fly ( Allobosca crassipes; 3 males, 8 females) in the dipteran family Hippoboscidae, which was collected during 26% (10/39) of lemur examinations (mean number/host=1.1). The lemur most heavily parasitized by mites was an adult female with 29 adult females and 17 nymphs, all collected from the face.

Zircon U-Pb age constraints for Tonian-early Cryogenian deposition of metasedimentary rocks from the Schirmacher Oasis, East Antarctica: Implications for correlations across the Mozambique Ocean

Schirmacher Oasis, central Dronning Maud Land, East Antarctica, a dispersed fragment of the 660-610 Ma East African Orogen (EAO), exposes platformal-facies high-grade metasedimentary rocks. Here we constrain the depositional age of these sedimentary rocks to test models relating to timing of the closure of the Mozambique Ocean, the southern segment of the EAO, since Tonian time. Zircon, a proxy for detritus, was dated by LA-MC-ICPMS (206Pb/238U ages) that includes both magmatic and (older) metamorphic populations, in addition to those formed during the granulite-facies metamorphic events (660-600 Ma and 580-555 Ma) having an age range between 1240 and 500 Ma. In conjunction with field evidence, their depositional time is constrained to the Neoproterozoic (Tonian to early Cryogenian, i.e. 1000 >Tdep >800 Ma and 930 >Tdep>800 Ma) with their provenance likely dominated by metamorphic (orogenic) over magmatic rocks.These metacarbonate and psammitic rocks are correlated with similar rocks from the Montepuetz Complex, Northeastern Mozambique, metacarbonate rocks from the Sør Rondane Mountains and with the Linta Group, Vohibory Domain, Southeastern Madagascar. The coeval deposition of platformal sedimentary protoliths, interpreted as occurrence of multiple Mozambique Oceanic strands, were incorporated into the continental arc crust during collision between the Congo and Azania cratonic blocks forming the EAO.

Exploring non-invasive sampling of parasites by metabarcoding gastrointestinal nematodes in Madagascar frog species

The diversity of Anuran parasites is poorly surveyed, despite arguably being one of the most important threats to anuran populations worldwide. Additionally, parasites also interact with a number of other stressors, such as invasive species, pollution, sedimentation and changing light conditions, caused by anthropogenic disturbance in natural habitats. We aimed to explore the use of metabarcoding, a new, non-invasive tool to survey the parasite assemblages in frogs in different environments facing different levels of anthropogenic pressure. We collected fecal samples from frogs across three different transects in Ranomafana National Park, located in southeastern Madagascar, and then used the 18S metabarcoding technique to identify nematode species from the collected fecal samples. We were able to find four different putative species, which were all identified to the genus level. In comparison to the literature on previous surveys done with traditional methods, the metabarcoding approach seems to provide similar diversity estimates and taxonomical accuracy. Our results suggest that non-invasive sampling and metabarcoding can provide a suitable tool for intestinal parasite surveys in anuran host populations.

Formation and evolution of the Chain-Kairali Escarpment and the Vishnu Fracture Zone in the Western Indian Ocean

Published models for the plate tectonic evolution of the Western Indian Ocean suggest that the Southern Mascarene Basin opened by oceanic crustal accretion between the continental margins of southwestern India and southeastern Madagascar. However, with the cessation of the Mascarene Basin spreading centre followed by a ridge jump resulting in the opening of the Carlsberg Ridge, almost all the traces of India-Madagascar divergence were carved away from the Indian Plate and attached to the African Plate. According to some recent studies, the Chain-Kairali Escarpment, a prominent feature on the southwestern continental margin of India, is possibly the only trace of India-Madagascar divergence that remained on the Indian Plate. But the exact conjugate correspondence of this feature on the Madagascar side is uncertain. Published plate tectonic reconstructions imply that the Vishnu Fracture Zone on the Indian side and the Mauritius Fracture Zone on the Madagascar side are aligned at chron C22ny (∼49.04 Ma). Based on the near collinearity of gravity anomaly trends, the Chain-Kairali Escarpment appears to be the landward extension of the Vishnu FZ. However, at chron C34ny (∼83.0 Ma), the Chain-Kairali Escarpment was in close proximity to the incipient Mahanoro Fracture Zone. In this study we investigate this incompatibility, using an up-to-date compilation of the tectonic elements from the conjugate regions of India and Madagascar and the latest available rotation parameters that describe India-Madagascar separation through a direct India-Seychelles-Madagascar plate circuit. Our revised plate reconstruction model suggests that the Chain-Kairali Escarpment was formed due to the strike-slip motion between the southeast coast of Madagascar and the then southwest coast of India during the initial stages of India-Madagascar breakup. The migration of the Chain-Kairali Escarpment from the proximity of the Mahanoro FZ and aligning with the Vishnu FZ was the result of several successive events. The first among those events was asymmetric crustal accretion in the Mascarene Basin due to ridge propagation, between chrons C34ny (83.0 Ma) to C33ny (∼73.62 Ma). The Chain-Kairali Escarpment and associated crustal weak zones offshore India appear to have facilitated subsequent initiation of the Mauritius FZ and its conjugate Vishnu FZ during a plate reorganization at about chron C33ny (∼73.62 Ma). The cessation of spreading in the Mascarene Basin and development of full extent of the Carlsberg Ridge, shortly after chron C27ny (60.92 Ma), resulted in the initiation of a long transform fault, coinciding with the Vishnu FZ, which connected the Carlsberg Ridge with the spreading centre of the Madagascar Basin. Therefore, the Vishnu FZ and the Chain-Kairali Escarpment are two independent features created during different episodes of evolution of the Western Indian Ocean and the Chain-Kairali Escarpment is not a landward extension of the Vishnu FZ.

Gender challenges in horticultural research in Ethiopia and Madagascar

Female farmers are often overburdened with the multiple roles they assume in households, farming, childcare and the community. Horticultural projects that aim to improve nutrition and livelihoods usually address the women's domain of home gardens and vegetable farming. As a consequence, interventions tend to increase the workload of female farmers and run the risk of negatively affecting the well-being of women as well as the nutritional status of households. This paper explores gender challenges of horticultural projects using two case studies from Ethiopia and Madagascar, where an attempt is made to promote horticultural production in agroforestry systems without increasing the burden on already overstrained women farmers. Data were collected through ethnography, 42 gender-disaggregated focus group discussions and 25 in-depth interviews with smallholder farmers, as well as 19 key stakeholder interviews in four village sites in Oromia, Ethiopia, and two sites in south-eastern Madagascar. The do-no-harm framework was used as the conceptual framework. It identifies so-called connectors and dividers in households and communities to prevent “gender harms”, to offset the negative effects of interventions, and to develop mitigation strategies to improve project outcomes. Research results showed that many factors can become connectors or dividers depending on the project objectives and approach. In both locations, vital dividers for the project are the immense workload of women that prevents the expansion of vegetable cultivation and the low value that is attached to (indigenous) vegetable and fruit crops, especially by men, which does not match with their important role for diet and family nutrition. Other dividers are male-dominated extension services, the executive role of women in horticulture and low access to inputs. Mitigation strategies that should be developed can include territorial, technological and training solutions that aim to change behavior.

Fruiting phenology is linked to rainfall variability in a tropical rain forest

AbstractAs the influence of climate change on tropical forests becomes apparent, more studies are needed to understand how changes in climatic variables such as rainfall are likely to affect tree phenology. Using a twelve‐year dataset (2005–2016), we studied the impact of seasonal rainfall patterns on the fruiting phenology of 69 tree species in the rain forest of southeastern Madagascar. We found that average annual rainfall in this region has increased by >800 mm (23%) during this period relative to that recorded for the previous 40 years and was highly variable both within and between years. Higher monthly measures of fruiting richness and the intensity of fruiting in our sample community were associated with significantly higher levels of rainfall. We also found that less rainfall during the dry season, but not the wet season, was associated with a significant shift toward later timing of peak richness and peak intensity of fruiting in the subsequent 12 months; however, this pattern was driven primarily by an extreme drought event that occurred during the study period. Longer time scales of phenology data are needed to see whether this pattern is consistent. Madagascar is expected to experience more extremes in rainfall and drought with increasing climate change. Thus, the linkages between variable precipitation and the fruiting phenology of forest trees will have important consequences for understanding plant reproduction and the ability of Madagascar's wildlife to cope with a changing climate.

Streptocarpus peltatus (Gesneriaceae), a Distinctive New Species from Southeastern Madagascar

Streptocarpus peltatus Randrian., Phillipson, Lowry & Mich. Moller (Gesneriaceae) is described as a new species from southeastern Madagascar. It differs from other Malagasy members of the genus by its distinctly peltate leaves that are glabrous on both surfaces and by the absence of staminodes. It is only known from a small area of mid-elevation humid forest on the eastern slopes of the Vohimena Range in southeastern Madagascar. An illustration and color images are provided along with a distribution map and a risk of extinction assessment following the IUCN Red List categories and criteria, which indicates a status of Critically Endangered.

Interannual Variation in Diet, Dietary Diversity, and Dietary Overlap in Three Sympatric Strepsirrhine Species in Southeastern Madagascar

Dietary data are used to categorize species diets, but these categorizations do not take into account the mutability of food resources in time or space, the level of interspecific competition in various communities as these resources change, nor the dietary flexibility of species. In this study, we assess the diets of three sympatric species, Eulemur rufifrons, Propithecus edwardsi, and Varecia variegata, in the Vatoharanana site in Ranomafana National Park, Madagascar. We determine dietary diversity, overlap, and interannual variation with data collected from 2001 to 2003. We then compare results on food preference and time feeding with data collected on each species in the late 1980s and early 1990s to determine whether these findings are consistent over the long term. We found little interannual variation in the proportion of time spent eating particular plant parts for each of the lemur species during the three study years (2001–2003), and between the earlier and current study. Food items were not always consumed based solely on availability. Dietary diversity was lower in the two frugivorous species (V. variegata and E. rufifrons) compared with the folivorous species (P. edwardsi), and V. variegata and E. rufifrons were more likely to focus their feeding time on one particular genus and plant part in each year. The study species used different strategies to deal with food, particularly fruit, shortages such as a plastic social structure (V. variegata), habitat shifting (E. rufifrons), and dietary switching (P. edwardsi). Although there was low dietary overlap between the study species, they depended on a small number of shared fruits in each of the study years (Chrysophyllum, Syzygium, Ocotea, Plagioscyphus), which may indicate some potential for interspecific competition. Because these lemur species, like all primates, lead relatively long lives (avg. >30 years) and have slow rates of aging, longitudinal studies are needed to test hypotheses reliant on basic dietary information.

Behaviour and Ecology of Male Aye-Ayes (Daubentonia madagascariensis) in the Kianjavato Classified Forest, South-Eastern Madagascar

Previous studies found that aye-ayes (Daubentonia madagascariensis) consume a variety of non-invertebrate resources, supporting the hypothesis that aye-ayes are too large-bodied to focus feeding efforts on insects. However, these conclusions were based on introduced populations, with little known about aye-aye behaviour and ecology in its natural habitat. This study investigates activity budgets, diet, and home range/territories of 2 male aye-ayes in the Kianjavato Classified Forest, a disturbed forest in south-eastern Madagascar, from October 2013 to October 2014. We used radiotelemetry and focal-animal sampling methods to collect behavioural data. We recorded GPS coordinates of the focal animal every 20 min, calculating the home range size for each individual. Results showed that male aye-ayes spent most of their time feeding and travelling. Their feeding time primarily focussed on larvae and adult insects from various substrates, and to a lesser extent Canarium spp. seeds. Home range sizes of the 2 males varied between individuals, overlapped greatly, and appeared to be related to the location of females. Our findings indicate that aye-aye behaviours are strongly indicative of insect-centric feeding ecology.

Persistence of Organophosphorus and Pyrethrinoid Insecticides, used for Malaria Control, in Soil and Water: Case of the District of Vohipeno, South-Eastern Madagascar

Malaria still persists in underdeveloped countries, including Madagascar, where it is the eighth leading cause of morbidity in Madagascar (SSS, 2011). Thanks to the application of the two insecticide-impregnated mosquito nets (ITN) and the Intra-Home Spraying Campaign (IDAC) systems, this rate has risen from 21.57% in 2003 to 5% in 2011 for children under 5 years of age and from 17.57% to 2.3% in 2011 for children over 5 years of age. The malaria mortality rate increased from 25.92% to 19% for children under 5 years of age and from 13.5% to 5% for those over 5 years of age (SSS, 2011). However, these control methods are based on the use of pesticides and require great caution. Indeed, due to poor practice, organophosphorus (insecticide used in CAID) and pyrethrinoid (insecticide impregnated on ITNs) were detected after analysis The instrument used was an Infinity 1290 Agilent Technologies HPLC Infinity 1290 system (Santa Clara, CA, USA) coupled with an Agilent Technologies 6460 triple quadrupole mass spectrometer in soil, surface water and groundwater from the three villages (Tanandava, Vohitramba and Savagna) in the south-eastern region of Madagascar where sampling takes place.

A new Gravesia (Melastomataceae, Sonerileae) from the Bemangidy-Ivohibe rainforest of southeastern Madagascar

Almeda, F. & H. Ranarivelo (2018). A new Gravesia (Melastomataceae, Sonerileae) from the Bemangidy-Ivohibe rainforest of southeastern Madagascar. Candollea 73: 1–5. In English, English and French abstracts. DOI: http//dx.doi.org/10.15553/c2018v731a1Gravesia parvula Almeda & Ranarivelo (Melastomataceae, Sonerileae), a new species from the Bemangidy-Ivohibe forest of southeastern Madagascar, is described, illustrated, mapped, and compared with superficially similar Malagasy species. In addition to its dwarf rosette-like habit, it is distinguished by the semi-succulent obovate to oblanceolate 1-nerved leaf blades with entire distally repand margins, indumentum of minute dark brown gland-like trichomes that are rounded to shallowly bilobed at the apex, stamens with external (antesepalous) and internal (antepetalous) whorls somewhat unequal in size in each flower, obovoid to oblong anther thecae with cleft-like ventrally inclined apical pores, and capitate stigma. A conservation assessment of “Endangered” is recommended for this species based on IUCN Red List Categories and Criteria.

Entamoeba histolytica infection in wild lemurs associated with proximity to humans

Amoebiasis, caused by Entamoeba histolytica, affects 50 million people worldwide, and results in 100,000 deaths annually. It is particularly prevalent in developing nations where poverty and poor sanitation contribute to contamination of food and water. E. histolytica is also a zoonotic protozoan parasite with the potential to infect non-human primates. Lemurs, primates endemic to Madagascar, are the most threatened mammalian group in the world due to habitat loss. As forests disappear, humans and lemurs come into more frequent contact, and the potential for E. histolytica to infect lemurs intensifies. Consequently, we screened 176 fecal samples from seven lemur species at eight sites in the rain forests of southeastern Madagascar for E. histolytica to determine if human proximity influenced lemur infection. Of samples examined, 4.0% (from three lemur species) were positive for E. histolytica. Of lemurs infected with E. histolytica, three (43%) exhibited diarrheal feces. Distance to human settlements explained the variation in E. histolytica infection seen in lemurs. These results provide the first evidence of E. histolytica in wild lemurs and highlight the need for additional work to better understand the eco-epidemiology of this potential threat to these species.

Comparative genetic structure in two high-dispersal prawn species from the south-west Indian Ocean

The penaeid prawns Fenneropenaeus indicus and Metapenaeus monoceros support shallow-water prawn fisheries in the south-west Indian Ocean. They are sympatric and have similar life histories, including developmental stages that depend on estuarine and marine habitats and a short dispersal duration. Nevertheless, M. monoceros juveniles display a more generalist habitat preference in estuaries and recruit to offshore habitats during a different season than F. indicus. We hypothesised that these differences would affect dispersal patterns, leading to dissimilar geographic genetic structure between the two taxa. Given their short dispersal phase, we also hypothesised that the Mozambique Channel would form a barrier to dispersal between the southeastern African mainland and Madagascar sites. Population differentiation was assessed based on analysis of mitochondrial DNA control-region sequences. Both species displayed high haplotype and low nucleotide diversity. Pairwise ϕST statistics supported the existence of admixed populations along the African mainland sites for both species, with geographic distance isolating populations at the extremes of the sampled range (Kenya and east coast of South Africa). The Madagascar population differed significantly from African mainland populations. The concordant patterns in population differentiation suggest that F. indicus and M. monoceros can be considered as single African stocks, or fisheries management units.

A new species of smooth-skinned Spinomantis frog (Anura: Mantellidae) from south-eastern Madagascar

We present molecular evidence for the presence of two species morphologically similar to Spinomantis bertini in Andohahela National Park, south-eastern Madagascar, differing by 5.5−6.3% pairwise DNA sequence divergences in the mitochondrial 16S rRNA gene. One of these was observed at higher elevations of ca. 1650 m above sea level, whereas the other was found at lower elevations of ca. 715 m a.s.l., close to the type locality of S. bertini (Isaka-Ivondro), and in one other location (Andreoky, ca. 1050 a.s.l.). We herein assign these low- to mid-elevation specimens to S. bertini based on their occurrence near the type locality and general agreement in colour pattern with the type specimen of Gephyromantis bertini Guibé, 1947. The high-elevation form is described as Spinomantis beckei sp. nov. based on its molecular divergence and reciprocal monophyly with respect to S. bertini, lower expression of greenish dorsal colour and less distinct frenal stripe. Based on a comparison of published call descriptions for S. bertini and our recordings of S. beckei, we hypothesize that S. bertini has a lower note repetition rate in advertisement calls. Molecular data suggest that the S. bertini species complex is more diverse than previously recognized, with at least two more candidate species identified: S. sp. Ca7 from Ranomafana National Park, and a newly identified candidate species S. sp. Ca12 from Pic d’Ivohibe Special Reserve.

Tip-dated phylogeny of whirligig beetles reveals ancient lineage surviving on Madagascar

The temporal origin of Madagascar’s extraordinary endemic diversity is debated. A preference for Cenozoic dispersal origins has replaced the classical view of Mesozoic vicariance in the wake of molecular dating. However, evidence of ancient origins is mounting from arthropod groups. Using phylogenetic ‘tip-dating’ analysis with fossils, we show that a whirligig beetle species, Heterogyrus milloti, inhabiting forest streams in southeastern Madagascar is the last survivor of a once dominant and widespread Mesozoic group. With a Late Triassic to Early Jurassic origin (226–187 Ma) it is the hitherto oldest dated endemic lineage of animal or plant on Madagascar. Island biotas’ sensitivity to extinction is well known, but islands can also provide refuge from continental extinction. Heterogyrus milloti is an irreplaceable link to the freshwater biota of the Mesozoic and serves as a reminder of what may be lost without critical conservation efforts on Madagascar.

Comparing the use of live trees and deadwood for larval foraging by aye-ayes (Daubentonia madagascariensis) at Kianjavato and Torotorofotsy, Madagascar

Aye-aye (Daubentonia madagascariensis) feeding behavior has become synonymous with deadwood foraging. However, deadwood is not always the most frequently used substrate, as some aye-ayes use live trees more often to access invertebrates. We sought to compare the frequency of aye-aye invertebrate foraging in deadwood and live trees to better understand their feeding behaviors. We followed two male aye-ayes at Kianjavato, a heavily disturbed habitat in southeastern Madagascar, from October 2013 to October 2014, and one male and one female aye-aye at Torotorofotsy, a continuous forest in eastern Madagascar, from July 2014 to December 2015. We collected feeding data by recording the behavior of a focal aye-aye every 5min for a total of 373h at Kianjavato and 383h at Torotorofotsy. Our results showed no difference in the amount of deadwood used between the individuals. However, there was a significant difference in the amount of live tree feeding between the female at Torotorofotsy and one of the males at Kianjavato. We conclude that feeding on invertebrates in live trees is more important to aye-ayes than previously realized and that aye-ayes are exceedingly flexible in their invertebrate feeding behaviors, adjusting to their habitat by using various substrates.

Of lemurs and louse flies: The biogeochemical and biotic effects of forest disturbance on Propithecus edwardsi and its obligate ectoparasite Allobosca crassipes in Ranomafana National Park, southeastern Madagascar.

From alleles to ecosystems and landscapes, anthropogenic activity continues to affect the environment, with particularly adverse effects on biodiversity hotspots such as Madagascar. Selective logging has been proposed as a "win-win" conservation strategy, yet its effects on different components of biodiversity are still not fully understood. Here we examine biotic factors (i.e., dietary differences) that may be driving differences in biogeochemical stocks between disturbed and undisturbed forests. We present the stable nitrogen (δ15 N) and carbon (δ13 C) isotope composition of hair from the lemur Propithecus edwardsi and of whole bodies of its obligate ectoparasite, the louse-fly Allobosca crassipes, from sites in Ranomafana National Park (RNP) that are comparable except for the history of logging and subsequent forest regeneration. P. edwardsi and A. crassipes from the disturbed (i.e., heavily selectively logged) site are lower in 15 N and 13 C relative to P. edwardsi and A. crassipes from sites that were minimally selectively logged or not commercially logged at all. There is a ∼3‰ decrease in 15 N between disturbed and undisturbed sites that corresponds to a difference of nearly a full trophic level. Flowers from Bakerella clavata, a staple food source for P. edwardsi in disturbed habitats and a fallback food for P. edwardsi in primary forests, were also analyzed isotopically. B. clavata is δ15 N-depleted in both disturbed and undisturbed sites. Data from longitudinal behavioral surveys of P. edwardsi in RNP and other forests in eastern Madagascar point to significant differences in consumption patterns of B. clavata, with P. edwardsi in disturbed forests consuming almost twice as much of this plant. Depletion of 15 N in animal tissues is a complex issue, but likely the result of the interaction of physiological and ecological factors. Anthropogenic disturbance in RNP from selective logging has had both biotic and biogeochemical effects that are observable trophically.

Stuck in fragments: Population genetics of the Endangered collared brown lemur Eulemur collaris in the Malagasy littoral forest.

The Endangered collared brown lemur (Eulemur collaris) is the largest primate living in the littoral forest of southeastern Madagascar, a top priority habitat for biodiversity conservation on the island. Because this lemur is a key seed-disperser, an evaluation of the structure and connectivity of the populations surviving in the forest fragments is urgently needed to guide conservation plans. Genetic variability at autosomal microsatellites and mitochondrial DNA was investigated in a total of 49 collared brown lemurs sampled by non-invasive methods in three littoral forest fragments and in the nearby lowland humid forest. The overall genetic diversity of E. collaris in the southeastern coastal region of Madagascar was lower than in other populations, as well as in other lemur species. The population appears highly structured, with less variable and more inbred groups inhabiting the littoral forest fragments compared to the inland area. Major barriers to gene flow were identified isolating littoral forest fragments from each other and from the inland lowland humid forest. Medium to long-term drift and scarce gene flow is the scenario that best explains the current genetic distribution. Habitat discontinuities such as rivers and grassland between forest fragments played a major role in structuring the population. A common history of size contraction is pointed out by several genetic estimators, indicating a possible ecological crisis triggered around 1,300 years ago. The adoption of strategies aimed at facilitating gene flow and population growth appears crucial to delay further loss of genetic diversity.