Wadi Khuda-Wadi Shut area in the South Eastern Desert (SED) of Egypt is mainly made up of gneisses, ophiolitic serpentinites and intermediate schistose metavolcanics. These metamorphic rock units are intruded by syn-late-tectonic diorite, granodiorite, ultramafic-mafic, and monzogranite intrusions. The gneisses comprise biotite hornblende gneiss, hornblende gneiss and migmatites. They are of granodiorite and diorite composition. The ophiolitic serpentinite with talc carbonate rocks were thrusted over the metavolcanics with NW-SE thrust contact. The late tectonic ultramafic-mafic rock units are represented in the study area by Dahanib pluton, which is consisted of layered peridotite-dunite associated with olivine gabrro, normal gabbro and gabbro-norite as well as rare hornblende gabbro. These varieties are related to the layered intrusions rather than Alaskan-types, and formed by magmatic differentiation. The granodiorite and monzogranite represent the youngest basement rock units in the study area. The principal components and band ratios of ASTER and Landsat-8 data were successfully used for the first time in lithological discrimination and geological mapping of the study area. This study concluded that, the interpreted data of ASTER and Landsat-8 combined with field study and petrographic investigation clearly discriminated the exposed rock units. Accordingly, a detailed geological map emphasizing the lithological units in Khuda-Shut area has been presented.