Southeast United States Research Articles

Mesoscale Influences of Land Use, Topography, Antecedent Rainfall, and Atmospheric Conditions on Summertime Convective Storm Initiation under Weak Synoptic-Scale Forcing

Abstract Throughout the summer months in the Southeast United States (SEUS), the initiation of isolated convection (CI) can occur abundantly during the daytime with weak synoptic support (e.g., weak wind shear). Centered around this premise, a dual-summer, limited-area case study of CI events concerning both geographical and meteorological features was conducted. The goal of this study was to help explain SEUS summertime CI in weak synoptic environments, which can enhance CI predictability. Results show that spatial CI nonrandomness event patterns arise, with greater CI event density appearing over high elevation by midday. Later in the day, overall CI event counts subside with other mechanisms/factors emerging (e.g., urban heat island). Antecedent rainfall, instability, and moisture features are also higher on average where CI occurred. In a random forest feature importance analysis, elevation was the most important variable in dictating CI events in the early to midafternoon while antecedent rainfall and wind direction consistently rank highest in permutation importance. The results cumulatively allude to, albeit in a muted, nonsignificant statistical signal, and a degree of spatial clustering of CI event occurrences cross the study domain as a function of daytime heating and contributions of features to enhancing CI probabilities (e.g., differential heating and mesoscale thermal circulations). Significance Statement Widespread isolated thunderstorms in the Southeast United States summer season with weak synoptic support have been commonly observed. With forecasting these remaining a challenge, a dual-summer intercomparison of geographical/meteorological features with convective initiation events was conducted. Radar data with a minimum threshold for convective initiation detection (35 dBZ) were used. Spatial nonrandomness was discovered with greater event density appearing over higher elevation by midday. Features such as prior rainfall and atmospheric instability/moisture were higher on average where initiation occurred. In a feature importance analysis, elevation ranked higher in the early to midafternoon hours while antecedent rainfall and wind direction ranked highest overall in permutation importance. These results allude to the contribution of localized phenomena to the nonrandomness (e.g., mesoscale circulations).

Studying U.S. college faculty during the COVID-19 pandemic: Perceptions of severity, concerns, sources of information, preventive behaviors, barriers to work performance, and impact on work productivity

This study examined perceptions of and experiences during the COVID-19 pandemic among 489 faculty from four public universities in the southeast United States. Data were collected via an online survey during the Fall 2020 semester, when campuses re-opened after closing in March. Two thirds of faculty perceived the severity of COVID-19 as severe, and their greatest concerns related to preventive behaviors, the possibility of a virus resurgence, and concern for the health of family/friends. The majority reported frequently engaging in COVID-19-preventing behaviors, which was highest among female and older faculty, and those with higher perceived severity and concern for personally contracting the virus. Over half of respondents reported teaching and scholarly activities were negatively impacted by the pandemic; most notable barriers to productivity included anxiety/stress and a distracted home environment. The study adds to the research on university faculty during the pandemic and aligns with research on the adult population.

They Were Here: A Study on High School Students’ Engagement in Historical Empathy With a Local History Research Project

In this study, we explored how high school students’ participation in a local history research project about a historically Black cemetery in the Southeast United States contributed to their demonstration of historical empathy. Major findings show that students displayed historical empathy in research activities that occur beyond the traditional classroom through their examination of perspectives concerning representations of race and diversity in the social studies curriculum, the historical contexts about the impact of enslavement and Jim Crow segregation in their community, and affective connections they made to their identities as citizens. We recommend that future studies focus on how social studies teachers, community organizations, and other educational providers can collaborate to promote historical empathy in and outside of schools with equity frameworks and curricular initiatives such as the National Council for the Social Studies College, Career, and Civic Life (C3) Framework.

Veterans’ lived experiences with the VA's Whole Health system and perceived impact on dimensions of wellness

Research questionWhat are the lived experiences and wellness related outcomes of veterans engaged in the Department of Veterans’ Affairs (VA) Whole Health (WH) system of care? Theoretical frameworkThis qualitative work was conducted with a pragmatic phenomenological approach to understand patients’ lived experience within the WH system of care. Data were contextualized within a multi-dimensional wellness model. MethodologyThis descriptive quality improvement project used semi-structured telephone interviews. Interview script elicited veterans’ WH participation experiences and perceived wellness related outcomes. ContextData were collected within a WH Service, at a large Veterans Health Administration Hospital in the Southeast United States. Sample selectionData were collected with a purposive sample of veterans that participated in at least 2 WH activities. Data collectionPatients were recruited by WH clinical team collaborators to participate in qualitative data collection. Analysis and interpretationRapid content analysis and interpretation of results were conducted in alignment with dimensions of wellness constructs. Main resultsWH offers veterans’ non-pharmacological tools to improve mental, physical, and social wellness. Participants (n = 50) represented the larger veteran population. Most veterans perceived a positive WH experience with improvement of three primary dimensions including mental and emotional, physical, and social wellness – impacts on other dimensions gleaned less perceived impact. Veterans reported adopting mindfulness and coping strategies, better mobility, pain management, and sleep quality, and enhanced social engagement. Even those who did benefit personally from all aspects of WH, felt the services are needed to support the larger veteran population. Reduced suicidal ideation and pain medication use emerged as a WH effect among approximately 10% of the sample.

English

Abstract The southeast United States is the epicenter of global crayfish biodiversity, but these species are among some of the most threatened in the world. Life history and biological trait data deficiencies, particularly of burrowing crayfish species, hampers efforts for accurately assessing imperilment rates and informing conservation efforts. Rising temperatures are a threat to aquatic ectotherms and it is important to evaluate crayfish tolerances to determine if they are at or near their upper thermal limits. We conducted dynamic temperature ramps to evaluate the temperatures at which ecological death (critical thermal maximum; CTM) and physiological death (upper thermal limit; UTL) occurred for the imperiled and narrowly endemic primary burrower Cambarus harti and three common, sympatric species C. latimanus, C. striatus, and Procambarus clarkii. All species differed in CTM with C. latimanus being the most sensitive (35.0°C), C. striatus (37.0°C) and C. harti (37.8°C) having intermediate tolerance, and P. clarkii being the most tolerant (39.0°C). The UTL of all species ranged from 39.4 – 39.8°C. We found no evidence that C. harti, a rare primary burrower, will require more restrictive acute thermal guidelines for protection compared to more common sympatric secondary burrowers.

Ground-penetrating radar (GPR) survey and spatial analysis of the George and Addie Giddens Cemetery, Opelika, Alabama

ABSTRACT Interest in locating and preserving the cemeteries of enslaved African Americans has increased the need for new methodologies coupled with efficient, noninvasive geophysical techniques, such as ground-penetrating radar (GPR). The southeast United States, where many of such sites are located, provides many challenges to using GPR due to its humid climate and abundant vegetation, which deteriorates burial remains, disguises burial shafts, and forms physical obstacles to GPR surveys. These challenges make it difficult for GPR alone to locate caskets or burial shafts. To address these issues, this study uses GPR combined with an innovative spatial and observational methodology to locate burials in a humid climate and determine their burial conditions. These mixed methods led to the location of 129 potential graves. Knowing the spatial distribution of burial features provides a context for analyzing the relative burial ages and decay rates within the cemetery site.

Limitations of Noninvasive Tests-Based Population-Level Risk Stratification Strategy for Nonalcoholic Fatty Liver Disease.

Nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) are highly prevalent but underdiagnosed. We used an electronic health record data network to test a population-level risk stratification strategy using noninvasive tests (NITs) of liver fibrosis. Data were obtained from PCORnet® sites in the East, Midwest, Southwest, and Southeast United States from patients aged [Formula: see text] 18 with or without ICD-10-CM diagnosis codes for NAFLD, NASH, and NASH-cirrhosis between 9/1/2017 and 8/31/2020. Average and standard deviations (SD) for Fibrosis-4 index (FIB-4), NAFLD fibrosis score (NFS), and Hepatic Steatosis Index (HSI) were estimated by site for each patient cohort. Sample-wide estimates were calculated as weighted averages across study sites. Of 11,875,959 patients, 0.8% and 0.1% were coded with NAFLD and NASH, respectively. NAFLD diagnosis rates in White, Black, and Hispanic patients were 0.93%, 0.50%, and 1.25%, respectively, and for NASH 0.19%, 0.04%, and 0.16%, respectively. Among undiagnosed patients, insufficient EHR data for estimating NITs ranged from 68% (FIB-4) to 76% (NFS). Predicted prevalence of NAFLD by HSI was 60%, with estimated prevalence of advanced fibrosis of 13% by NFS and 7% by FIB-4. Approximately, 15% and 23% of patients were classified in the intermediate range by FIB-4 and NFS, respectively. Among NAFLD-cirrhosis patients, a third had FIB-4 scores in the low or intermediate range. We identified several potential barriers to a population-level NIT-based screening strategy. HSI-based NAFLD screening appears unrealistic. Further research is needed to define merits of NFS- versus FIB-4-based strategies, which may identify different high-risk groups.

ENSO and MJO Modulation of U.S. Cloud-to-Ground Lightning Activity

Abstract Cloud-to-ground (CG) lightning substantially impacts human health and property. However, the relations between U.S. lightning activity and the Madden–Julian oscillation (MJO) and El Niño–Southern Oscillation (ENSO), two predictable drivers of global climate variability, remain uncertain, in part because most lightning datasets have short records that cannot robustly reveal MJO- and ENSO-related patterns. To overcome this limitation, we developed an empirical model of 6-hourly lightning flash count over the contiguous United States (CONUS) using environmental variables (convective available potential energy and precipitation) and National Lightning Detection Network data for 2003–16. This model is shown to reproduce the observed daily and seasonal variability of lightning over most of CONUS. Then, the empirical model was applied to construct a proxy lightning dataset for the period 1979–2021, which was used to investigate the summer MJO–lightning relationship at daily resolution and the winter–spring ENSO–lightning relationship at seasonal resolution. Overall, no robust relationship between MJO phase and lightning patterns was found when seasonality was taken into consideration. El Niño is associated with increased lightning activity over the coastal Southeast United States during early winter, the Southwest during winter through spring, and the Northwest during late spring, whereas La Niña is associated with increased lightning activity over the Tennessee River valley during winter. Significance Statement Cloud-to-ground lightning is dangerous for humans via direct strikes or through triggering wildfires, generating air pollution, etc. How lightning activity can be affected by climate remains unclear, and it is challenging to study their links because the data record for lightning is short. With the available lightning record, we developed a model that relates lightning flash counts over the United States to environmental factors. This model well represents observed fluctuations in daily and seasonal lightning over most of the United States. Because the model only needs environmental information to predict lightning flash counts, we were able to construct a longer record of predicted lightning based on the longer data record of environmental variables. With this dataset, we investigated the links between lightning and climate and found that the state of sea surface temperatures in the tropical Pacific (El Niño–Southern Oscillation) is linked to changes in U.S. lightning patterns in winter and spring.

507. Impact of COVID-19 variant prevalence on hospitalization rate among individuals receiving monoclonal antibodies within a community health-system

Abstract Background During the COVID-19 pandemic, multiple targeted monoclonal antibodies were used in high risk patients with early COVID-19 to prevent complications. Serving as the primary referral site for a large geographic region in central North Carolina, our rural community health-system administered monoclonal agents to COVID-19 positive patients from December 2020 to November 2022. During this time period three predominate variants emerged (alpha, delta and omicron). Monoclonal Antibody Infusions at FirstHealth of the Carolinas, December 2020-November 2022 Methods A retrospective study was conducted at FirstHealth of the Carolinas Moore Regional Hospital examining patients from December 1st, 2020 to November 30th, 2022. All COVID-19 positive patients receiving one of the COVID-19 monoclonal therapies granted emergency use authorization during the study period were included. Patients were sub-divided into three distinct time periods representing different variants based on the prevalent pathogen in our region: December 2020 – June 2021 (alpha); July 2021- December 2021 (delta); January 2022-November 2022 (omicron). Hospitalization rates post monoclonal treatment were compared between time periods. Results 1820 monoclonal antibody infusions for COVID-19 were administered within the FirstHealth of the Carolinas system in the period studied. Number of infusions were highest during delta with over 60% of the infusions occurring during this period. Hospitalization rates in this group were 3.5% (alpha), 3.5% (delta), and 2.0% (omicron). Vaccination status among individuals getting monoclonal antibody infusions changed over time based on changing infusion criteria and variant prevalence. Conclusion In a real world, rural, community health care setting in the Southeast United States - hospitalization rates for high-risk individuals receiving monoclonal antibody infusions for COVID-19 were similar to reported rates and varied by variant prevalence. This is may be due to alterations in variant severity as well as vaccination status of the population. Disclosures All Authors: No reported disclosures

Artificial neural network-empowered projected future rainfall intensity-duration-frequency curves under changing climate

With the increasing trend of greenhouse gases in the atmosphere, by 2052 the temperature is expected to rise by 1.5 °C from the Pre-industrial Period, affecting future extreme rainfall events. This necessitates quantifying extreme hydrologic events to plan and design hydrologic and hydraulic structures using rainfall Intensity-Duration-Frequency (IDF) curves to adapt to future climate scenarios. This study developed future projected IDF curves for the Southeast United States using disaggregated sub-hourly (15-, 30-, and 45-min) monthly maximum rainfall from 2030 to 2059 using five climate models under the Representative Concentration Pathway 8.5 scenario. A computationally efficient feed-forward back-propagation Artificial Neural Network (ANN)-based approach was found to be significantly superior for disaggregating rainfall to a stochastic model with an average Nash–Sutcliffe efficiency (NSE) ranging from 0.67 to 0.84. The study found that there is an increasing rate of future projected annual maximum rainfall intensities in the range of 7% to 36% with reference to the historical period. The spatial variation in future projected extreme rainfall depths showed that the Gulf-Atlantic coast and the Appalachian Mountains are expected to receive more extreme rainfalls.

Evaluation of Pediatric Readiness Using Simulation in General Emergency Departments in a Medically Underserved Region.

Regionalization of pediatric care in the United States was developed to improve care by directing patients to hospitals with optimal pediatric resources and experience, leading to less pediatric-trained providers in medically underserved areas. Children with emergencies, however, continue to present to local general emergency departments (GEDs), where pediatric emergencies are low-frequency, high-risk events. The goals of this project were to: increase exposure of GEDs in the southeast United States to pediatric emergencies through simulation, assess pediatric emergency clinical care processes with simulation, describe factors associated with readiness including volume of pediatric patients and ED location (urban/rural), and compare these findings to the 2013 National Pediatric Readiness Project. This prospective in situ simulation study evaluated GED readiness using the Emergency Medical Services for Children Pediatric Readiness Score (PRS) and team performance in caring for 4 simulated pediatric emergencies. Comparisons between GED and pediatric ED (PED) performance and PRS, GED performance, and PRS based on pediatric patient volume and hospital location were evaluated. A Composite Quality Score (CQS) was calculated for each ED. Seventy-five teams from 40 EDs participated (39 GED; 1 PED). The PED had a significantly higher volume of pediatric patients (73,000 vs 4492; P = 0.003). The PRS for GEDs was significantly lower (57% [SD, 17] vs 98%; P = 0.022). The CQSs for all GEDs were significantly lower than the PED (55% vs 87%; P < 0.004). Among GEDs, there was no statistically significant difference in PRS or CQS based on pediatric patient volume, but urban GEDs had significantly higher CQSs versus rural GEDs (59.8% vs 50.6%, P = 0.001). This study shows a significant disparity in the performance and readiness of GEDs versus a PED in a medically underserved area. More education and better access to resources is needed in these areas to adequately care for critically ill pediatric patients.

Long‐term integration of bahiagrass into a cover‐cropped and strip‐tilled peanut–cotton rotation has a limited effect on soil carbon and other soil properties

AbstractIntegrating a perennial forage grass like bahiagrass (Paspalum notatum Flugge) into the traditional rotation of cotton (Gossypium hirsutum L.) and peanut (Arachis hypogaea L.) increases yields and reduces irrigation needs in the Southeast United States. However, the long‐term effects of this sod‐based rotation (SBR) on soil properties remain unclear. Eighteen years after establishing SBR and the traditional rotation, we collected samples to a depth of 120 cm in 30‐cm increments and measured soil carbon and other soil properties (e.g., Mehlich‐extractable nutrients). Soil C was 6% higher with SBR in the top 30 cm of soil after 18 years, with no impact on soil C in deeper soil layers. Similarly, there was no effect of SBR on other soil properties (soil texture, pH, and Mehlich‐extractable P and Mg), except for lower soil K in SBR. Our results indicate a limited effect of SBR on soil C and other properties in this system where SBR is not grazed and where strip tillage and cover cropping were implemented in both rotations.

Predicted and observed changes in summertime biogenic and total organic aerosol in the southeast United States from 2001 to 2010

Biogenic secondary organic aerosol (bSOA) is a major component of atmospheric particulate matter (PM2.5) in the southeast United States especially during the summer, when emissions of biogenic volatile organic compound (VOCs) are high and emissions from anthropogenic sources enhance the formation of secondary particulate matter. In this work we test the hypothesis whether a chemical transport model (PMCAMx) that includes a detailed description of gas-phase chemistry, secondary organic aerosol (SOA) formation based on the volatility basis set (VBS), and interactions among compounds based on partitioning theory can predict organic aerosol (OA) concentrations that are consistent with the observed changes in OA in response to significant changes in anthropogenic emissions during the summers of 2001 and 2010. The model had good performance for OA for both periods and its predictions were consistent with the observed changes in both the urban and rural areas. The fractional error of OA predictions remained practically the same (0.41 and 0.44 at Chemical Speciation Network (CSN) sites and 0.40 to 0.41 at Interagency Monitoring of Protected Visual Environments (IMPROVE) sites in the summers of 2001 and 2010 respectively) in the two examined periods. The fractional bias of OA predictions increased from 0.10 to 0.22 at CSN sites and decreased from 0 to −0.09 at IMPROVE sites between the two periods. Average predicted bSOA concentrations in the southeast US did not change appreciably from the summer of 2001 to the summer of 2010, while the anthropogenic SOA decreased by 45%. As a result, the biogenic fraction of predicted total OA increased from 0.46 in 2001 to 0.63 in 2010. Partitioning effects due to reduced anthropogenic OA from 2001 resulted in 0.4 μg m−3 less biogenic OA on average in the southeast US in the summer of 2010. This was offset by biogenic SOA increases due to higher biogenic vapor emissions in the warmer 2010 summer. Removing the NOx-dependence of SOA formation yields resulted in higher fractional error and fractional bias at both CSN and IMPROVE sites in both summer periods, demonstrating the efficacy of the current formulation of SOA yields. The results of the analysis support the conclusion that a CTM that simulates NOx-dependent SOA chemistry and semivolatile partitioning of SOA material can consistently predict the observed changes in a region rich in biogenic VOCs and SOA.

Abstract 14531: Higher Urban Health Index is Associated With Lower Ambulatory Blood Pressure in People Living in Southeast United States

Introduction: Hypertension is common in the Southeastern (SE) United States (US), which increases the risk of heart attack, stroke and other serious health problems. Ambulatory blood pressure (ABP) monitoring is the gold standard for diagnosing hypertension and provides better information on cardio- and cerebrovascular risk than office blood pressure (BP). However, the role of social determinants of health (SDOH) and their influence on BP elevation has been scarcely examined on a neighborhood level. The Urban Health Index (UHI), developed by the World Health Organization, has the potential to measure disparities in SDOH at the small areal level and examine inequalities in health outcomes. Hypothesis: Higher UHI is associated with lower blood pressure. Methods: We analyzed data using a longitudinal cohort established in 1989 with 724 individuals (age 5-16 in 1989, 50.8% females, 47.1% African Americans) from the Central Savannah River Area. A census tract level UHI was calculated using indicators of employment, income, and educational attainment. The UHI is a unitless and continuous measure (between 0 and 1). The relationship between UHI (2000 and 2010 data) and ABP was examined using a linear regression model, adjusted for age, sex, race, and family socioeconomic status. Results: Higher UHI was associated with lower ABP, with a 3.15 mmHg and 2.72 mmHg drop in 24-hour systolic and diastolic ABP with 1 unit increase in UHI, respectively (2000 data; p=0.010; p=0.020). The 2010 data showed similar trends, with an increase in UHI linked to a 2.66 mmHg decrease in mean 24-hour systolic ABP (P=0.042), with the diastolic ABP reduction (1.74 mmHg) in the same direction though not statistically significant (p=0.088). Conclusions: This is one of the first studies to delineate the correlation of ABP with UHI. Our results show that a higher UHI is associated with lower systolic and diastolic ABP, underscoring the influence of aggregate indicators of SDOH on individual health outcomes. Our results support the utility of the UHI as a tool to inform health policymakers on disparities on a sub-county level thereby informing targeted interventions to address these disparities.

370 Evaluation of Nutrient Digestibility By Calves Born from Early and Late Shedding Angus Dams

Abstract Researchers have investigated several factors that could alter fetal growth, including nutrient restriction (Valiente et al., 2021), hair shedding (Gray et al., 2011), and extreme hot and cold temperatures (Davidson et al., 2022). Hot temperatures and increased humidity percentages in the southeast United States caused researchers to investigate the hair coats of Angus cattle in the commercial production setting. An improvement in fiber digestibility and calf birth and weaning weights has been observed in Angus dams that shed 50% of the winter hair coat by May (Gray et al., 2011; Burnett et al., 2021). Our objective of this experiment was to investigate the nutrient digestibility of Angus calves born to cows that on average, shed early compared with calves from cows that shed later. Newly weaned, purebred Angus bull calves (early; n = 6, late; n = 6) were housed in metabolism crates for 10 d. Before the trial, calves had a 14-d adaption period to a 14% CP textured feed (CPC 14% Developer, CPC Commodities, Fountain Run, KY) and offered ad libitum Cynodon dactlyon hay and water. After 3 d crate acclimation period, urine, feces, orts, and hay samples were collected for 7 d. Concentrate was offered at 0.25% of average BW. Approximately 5% samples were taken of feces, and urine samples had 1 to 1.5% of 25% metaphosphoric acid added to prevent ammonia volatilization, and both collections were composited by animal. Orts were collected at 0600 h daily, dried, and composited by animal. Laboratory analysis included dry matter (DM), organic matter (OM), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), Kjeldahl N (CP), and fat. Data were analyzed using the GLM procedure of SAS 9.4 in a completely randomized design with calf as the experimental unit. Significant (P ≤ 0.05) means were separated using Fischer’s protected LSD. The model for intake included average daily DM and OM (kg) and adjusted by body weight (BW%). For digestibility analysis, the model included: DM, OM, ash, NDF, ADF, hemi-cellulose (HC), CP, and fat. The N retention model included: N retained (g/d), N retained/consumed (%), and N retained/DM intake (%). There were no differences between early or late calves for DM intake (5.502 ± 0.2774 kg/d; 2.251 ± 0.1247 %BW), or OM intake (5.199 ± 0.2591 kg/d; 2.128 ± 0.1166 %BW). There were no differences in digestibility for either group for DM, OM, Ash, NDF, ADF, HC, CP, or fat (Table 1.). There were also no differences in N retention in either group of calves (3.686 ± 2.0242 g/d; 4.366 ± 2.3964 %; 0.064 ± 0.0355 %). Further research is needed in this area to adequately understand factors influencing nutrient digestibility in calves born from early and late shedding dams.

Biodiversity connections—‘ties that bind’

AbstractConnectivity is a foundational concept in ecology and conservation and was the organising theme for the 2022 Annual Meeting of the Southeastern Fishes Council, a professional organisation dedicated to the study and conservation of freshwater fishes native to the southeast region of the United States (US). We introduce a Special Contribution of five papers selected from presentations at that meeting that illustrate perspectives on connections created by fish migration and dispersal, evolved life histories and habitat affinities and interspecific facilitation. Although focused on streams of the southeast US, each of these topics is broadly relevant to freshwater fish conservation, particularly with respect to causes and consequences of migratory fish depletion, population fragmentation and species declines. Many other connections relevant to the ecology and conservation of freshwater fishes remain relatively unexplored but could substantively advance conservation. We highlight the potential that species evolutionary histories, that is connections through time, reconstructed using species distributions and phylogenies may improve predictions of species responses to environmental change. Identifying species interdependencies, including undiscovered interactions that support survival or reproduction, could provide insights into how species losses may cascade as aquatic communities unravel. Finally, efforts to elucidate diverse connections between people and freshwater biodiversity, particularly where fisheries are historic and streams mostly go unnoticed, may prove essential to building public support for conservation measures. A research agenda anchored on ‘biodiversity connections’ has the potential to advance ecological understanding and public engagement, elements essential to conserving freshwater fishes.

Perception and acceptance towards water reuse in the Southeast United States: A public survey

The growing global water crisis has increased the need for alternative water sources. Among the potential solutions to mitigate the current challenging situation, water reuse has been considered and implemented, especially during the past decade. Public perception/acceptance represents one of the most common challenges related to the wide implementation of water reuse. To evaluate its public awareness and better understand the reasons behind the public skepticism for outdoor and indoor activities, a survey was conducted in the Southeast United States. The survey included open-ended questions, questions with five-point Likert scales, and multiple-choice questions. A total of 203 responses were considered for the final analysis. Participants, mostly in the 18–60 age range, hold at least a high school diploma (94 %). The age of the participants had an association with the knowledge related to the wastewater treatment method adopted in their household (chi squared, X2 = 28.8, p = 0.055). Participants 1) selected the U.S. EPA as the most credible organization in the field of drinking water, 2) were more willing to use recycled water for removing road dust, watering private yards or non-edible crops, public toilets, and firefighting than for watering edible crops/orchards or livestock feed, and 3) were reluctant to use recycled water when direct-human contact or ingestion can occur. While only 8 % were willing to use recycled water for drinking, the addition of further treatments combined with environmental regulations, frequent sampling campaigns, and educational activities highlighting possible treatments and potential benefits related to water reuse, will increase the participants' willingness to implement water reuse more widely.

Relation between Baroclinity, Horizontal Vorticity, and Mesocyclone Evolution in the 6–7 April 2018 Monroe, Louisiana, Tornadic Supercell during VORTEX-SE

Abstract This case study analyzes a tornadic supercell observed in northeast Louisiana as part of the Verification of the Origins of Rotation in Tornadoes Experiment Southeast (VORTEX-SE) on 6–7 April 2018. One mobile research radar (SR1-P), one WSR-88D equivalent (KULM), and two airborne radars (TAFT and TFOR) have sampled the storm at close proximity for ∼70 min through its mature phase, tornadogenesis at 2340 UTC, and dissipation and subsequent ingestion into a developing MCS segment. The 4D wind field and reflectivity from up to four Doppler analyses, combined with 4D diabatic Lagrangian analysis (DLA) retrievals, has enabled kinematic and thermodynamic analysis of storm-scale boundaries leading up to, during, and after the dissipation of the NWS-surveyed EF0 tornado. The kinematic and thermodynamic analyses reveal a transient current of low-level streamwise vorticity leading into the low-level supercell updraft, appearing similar to the streamwise vorticity current (SVC) that has been identified in supercell simulations and previously observed only kinematically. Vorticity dynamical calculations demonstrate that both baroclinity and horizontal stretching play significant roles in the generation and amplification of streamwise vorticity associated with this SVC. While the SVC does not directly feed streamwise vorticity to the tornado–cyclone, its development coincides with tornadogenesis and an intensification of the supercell’s main low-level updraft, although a causal relationship is unclear. Although the mesoscale environment is not high-shear/low-CAPE (HSLC), the updraft of the analyzed supercell shares some similarities to past observations and simulations of HSLC storms in the Southeast United States, most notably a pulse-like updraft that is maximized in the low- to midlevels of the storm. Significance Statement The purpose of this study is to analyze the airflow and thermodynamics of a highly observed tornado-producing supercell. While computer simulations can provide us with highly detailed looks at the complicated evolution of supercells, it is rare, due to the difficulty of data collection, to collect enough data to perform a highly detailed analysis on a particular supercell, especially in the Southeast United States. We identified a “current” of vorticity—rotating wind—that develops at the intersection of the supercell’s rain-cooled outflow and warm inflow, similar to previous simulations. This vorticity current develops and feeds the storm’s updraft as its tornado develops and the storm intensifies, although it does not directly enter the tornado.

No evidence to support the use of glycerol-oxalic acid mixtures delivered via paper towel for controlling Varroa destructor (Mesostigmata: Varroidae) mites in the Southeast United States.

A significant amount of researcher and practitioner effort has focused on developing new chemical controls for the parasitic Varroa destructor mite in beekeeping. One outcome of that has been the development and testing of "glycerol-oxalic acid" mixtures to place in colonies for extended periods of time, an off-label use of the otherwise legal miticide oxalic acid. The majority of circulated work on this approach was led by practitioners and published in nonacademic journals, highlighting a lack of effective partnership between practitioners and scientists and a possible failure of the extension mandate in beekeeping in the United States. Here, we summarize the practitioner-led studies we could locate and partner with a commercial beekeeper in the Southeast of the United States to test the "shop towel-oxalic acid-glycerol" delivery system developed by those practitioners. Our study, using 129 commercial colonies between honey flows in 2017 split into 4 treatment groups, showed no effectiveness in reducing Varroa parasitism in colonies exposed to oxalic acid-glycerol shop towels. We highlight the discrepancy between our results and those circulated by practitioners, at least for the Southeast, and the failure of extension to support practitioners engaged in research.

Mechanical, physical and compositional effects of Meruliporia incrassata on Southern Yellow Pine

Abstract With the growing risk of fungal degradation in timber-framed structures from significant moisture intrusion events due to climate change, it is increasingly critical to develop mechanistic relationships between fungal degradation mechanisms and the strength of untreated wood components. While extensive work has been performed characterizing wood decay, no study has yet addressed the effects of Meruliporia incrassata on untreated Southern Yellow Pine. This seeks to address this knowledge gap by evaluating the effects of the common brown rot fungus on an ubiquitous building material in the southeast United States – Southern Yellow Pine. Properties of Southern Yellow Pine were evaluated at seven decay stages over the course of 12 weeks of exposure to M. incrassata. Changes in physical properties – mass, density, and moisture content – were measured at each stage. Changes in stiffness were characterized via ultrasonic pulse velocity testing, and thermogravimetric analysis was utilized to assess compositional changes. The study found rapid and significant losses in stiffness at decay stages as early as four-weeks. Hemicellulose and cellulose degradation occurred steadily throughout the decay period. These results can be utilized to develop a stronger understanding of the mechanical behavior of timber-framed structures in the United States degraded by brown rot.