Southwestern Kenya Research Articles

DEMYSTIFYING AGRICULTURAL POPULISM IN MAASAI RANGELANDS IN THE SOUTHWESTERN KENYA

Agricultural expansion in Kenya’s Amboseli region has resulted in proven negative effects both on native megafauna that use the ecosystem as a dispersal area, as well as on grazing and water resources utilized by local livestock herds. While community conservancy programs supported by non-governmental organizations (NGOs) provide an ecologically sustainable alternative to farming’s increasing popularity, the claim that agriculture is the most profitable land-use option for the region remains dominant –– even as increasingly frequent droughts interrupt agricultural processes. This study presents a comparative analysis of the overall benefit of land-use– agriculture and conservancies– in the Amboseli region. We draw on fieldwork conducted between March - May 2023, in the former Kimana Group Ranch in the Amboseli region of southwestern Kenya. Data was collected through household surveys with 150 landowners, and key informant interviews with leaders and officers of various conservancies and NGOs in the area. We conducted approximate analyses of the annual cash flow reported by each household to compare the profitability of different land-use options. The results of the study show that, while agriculture is by far the most popular land-use option in Amboseli, the region’s traditional pastoralist practices remain the most financially stable and lucrative land-use option. Leasing land for conservation through community conservancies was the second most profitable. Irrigated agriculture, mostly practiced by non-Maasai people, showed relatively short-lived financial returns. We argue that increased farming in Maasai rangelands is fueled primarily by land privatization and the promise of immediate profits –– promises whose benefits are far outweighed by long-term economic and socio-ecological consequences. Considering that indigenous Maasai pastoralism is most sustainable for the semiarid rangeland and that our research indicated agriculture is practiced most by non-Maasai, we argue for an examination of how traditional ecological knowledge (TEK) competes with non-indigenous practices, especially considering that rangelands in East Africa are increasingly becoming fragile due to climate change.<p> </p><p><strong> Article visualizations:</strong></p><p><img src="/-counters-/soc/0821/a.php" alt="Hit counter" /></p>

Assessing the Impacts of Climate Change on Geographical Distribution of Tea (Camellia sinensis L.) in Kenya with Maximum Entropy Model

Climate change has been disturbing the present species distribution ranges, resulting in the shifting of cultivation areas and decreases in production and quality. Tea (Camellia sinensis L.), which seeks optimum climatic resources, is a key cash crop economically in Kenya. In this study, the shifting of tea suitability was projected with the MaxEnt model under the SSP (SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5) climate scenarios for the 2050s and 2090s relative to the 1970–2000 distribution. Analysis under the current climatic condition showed that the proportions of optimal and medium- and marginal-suitable areas were 2%, 3%, and 24% of the total area, respectively, and located in south-western (SW), central, and north-eastern (NE) Kenya and, to some extent, in the Rift Valley. It was projected that the potential suitable tea-growing areas would migrate from the western areas to the central, eastern, and north-eastern highlands in Kenya. It was detected that the precipitation of the driest period (July), precipitation of the wettest quarter (April, May, and June), and annual temperature range could be the main climatic factors determining the shift in tea distribution. Compared to the current distribution (29%), the climatically suitable areas for tea production could reach 32.58% of Kenya’s land area under the SSP1-2.6 scenarios in the 2050s and 35.08% in the 2090s under the SSP5-8.5 scenario. On the contrary, it was found that the optimal climate-suitable habitats were projected to shrink by 2% and 1% in the 2050s and 2090s under all scenarios on the west side of the Great Rift Valley compared to the current distribution. In comparison, the sizes of medium- and marginal-suitable habitats would increase by 1% and 3%, respectively. The findings indicated that unless adaptive climate actions are taken, climate change could reduce the tea planting areas in western Kenya. Meanwhile, climate suitability was projected to expand upward on the east side of the Rift Valley, enhancing the potential distribution of tea. The developed climate information could be used to design and implement adaptation interventions in the lower elevation areas. Finally, we highlight that the available scientific literature on the climate suitability of tea in Kenya should be broadened by adding non-climatic factors.

Impacts of anthropogenic habitat modification on mammalian diversity in the Mau Forest Complex, Kenya

Habitat destruction is a major driver of biodiversity loss, especially in the tropics. We investigated changes in the diversity and occupancy of forest-dwelling mammals in response to habitat loss, alteration, and degradation at the Mau Forest Complex (MFC), southwestern Kenya. Using a systematic camera trapping grid, we estimated patterns of mammalian diversity, occupancy of small carnivores, and detection rates of functional mammalian groups across three habitat types (primary forests, secondary forests, and cultivated habitats). Between March 2019 and October 2021, 246 camera traps were deployed across 181 locations representing 5509 sampling days, resulting in 47,345 images of 52 mammalian species in nine mammalian orders. Alpha diversity differed among treatments, with primary forests having the highest diversity, and cultivated habitats the lowest, though results were not statistically significant. Beta diversity differed significantly among treatments, indicating differences in community composition as well as species richness. Overall occupancy for small carnivores was highest in primary forests and lowest in cultivated habitats, with some species specializing in primary forests while others were able to occupy more degraded habitats. Among functional groups, detection rates only differed significantly for large rodents, primates, and ungulates. Our results indicate that although functional groups and species may respond differently to habitat loss and alteration at the MFC, overall species diversity of specialist mammals was highest in the less-disturbed primary forests. Specialist, forest-dependent species are likely most affected by human activities, making them especially vulnerable to habitat loss at the MFC.

Perspectives of Pregnant Women Regarding the Use of Mobile Phones for Antenatal Health Education: A Qualitative Study in Rural Southwestern Kenya

Introduction: Pregnant women face a myriad of challenges ranging from anxiety, myths and misconceptions concerning pregnancy, diet, and general antenatal care because of inadequate health education during the antenatal period. There are extensive studies on mobile phone use in primary care; however, only a few qualitative studies have been conducted to establish the usefulness of these devices in antenatal education. Therefore, this study aimed to explore pregnant women’s views on mobile phone use in antenatal education. Methods: This study utilised a qualitative study design. It was conducted in rural health facilities in southwestern Kenya. Focus group discussions were used to collect data which were analysed thematically using ATLAS.ti version 23. Results: The overall theme that emerged from the study was “antenatal education is beneficial, and if provided via a preferred mode of mobile phone communication, can bridge the gap in antenatal education content.” The participants perceived antenatal education through mobile phones as beneficial and complementary to the information received during routine antenatal care. Most of the participants felt that antenatal education should be given through a preferred mode of mobile phone communication. Conclusion: Mobile phones have the potential to improve the quality of antenatal education provided during pregnancy.

An integrated hierarchical classification and machine learning approach for mapping land use and land cover in complex social-ecological systems

Mapping land use and land cover (LULC) using remote sensing is fundamental to environmental monitoring, spatial planning and characterising drivers of change in landscapes. We develop a new, general and versatile approach for mapping LULC in landscapes with relatively gradual transition between LULC categories such as African savannas. The approach integrates a well-tested hierarchical classification system with the computationally efficient random forest (RF) classifier and produces detailed, accurate and consistent classification of structural vegetation heterogeneity and density and anthropogenic land use. We use Landsat 8 OLI imagery to illustrate this approach for the Extended Greater Masai Mara Ecosystem (EGMME) in southwestern Kenya. We stratified the landscape into eight relatively homogeneous zones, systematically inspected the imagery and randomly allocated 1,697 training sites, 556 of which were ground-truthed, proportionately to the area of each zone. We directly assessed the accuracy of the visually classified image. Accuracy was high and averaged 88.1% (80.5%–91.7%) across all the zones and 89.1% (50%–100%) across all the classes. We applied the RF classifier to randomly selected samples from the original training dataset, separately for each zone and the EGMME. We evaluated the overall and class-specific accuracy and computational efficiency using the Out-of-Bag (OOB) error. Overall accuracy (79.3%–97.4%) varied across zones but was higher whereas the class-specific accuracy (25.4%–98.1%) was lower than that for the EGMME (80.2%). The hierarchical classifier identified 35 LULC classes which we aggregated into 18 intermediate mosaics and further into five more general categories. The open grassed shrubland (21.8%), sparse shrubbed grassland (10.4%) and small-scale cultivation (13.3%) dominated at the detailed level, grassed shrubland (31.9%) and shrubbed grassland (28.9%) at the intermediate level, and grassland (35.7%), shrubland (35.3%) and woodland (12.5%) at the general level. Our granular LULC map for the EGMME is sufficiently accurate for important practical purposes such as land use spatial planning, habitat suitability assessment and temporal change detection. The extensive ground-truthing data, sample site photos and classified maps can contribute to wider validation efforts at regional to global scales.

Caregivers’ Perceptions of Do Not Resuscitate Orders: A Qualitative Study in Rural Southwestern Kenya

The need for palliative care services has increased with the rise of patients with terminal illnesses. Unfortunately, there is a need for more research in palliative care, especially on end-of-life care. Conversations during that period present an ethical and cultural challenge in both collectivistic and individualistic societies. Worldwide, the laws regulating Do Not Resuscitate orders differ from country to country. The perceptions also vary from society to society. Studies have shown the need to involve families in this era of rising advocacy for patient-centered care. Caregivers have an essential role in decision-making and are involved in end-of-life decisions. This study aimed to describe caregivers' perceptions of Do Not Resuscitate orders. The study used a qualitative approach and phenomenological research design. It involved eighteen caregivers of patients receiving palliative care. The sampling procedure was non-probability purposive. Data was collected using in-depth interviews and analyzed using thematic analysis. Overall, two themes emerged from the findings. The first theme was a lack of awareness of the Do Not Resuscitate orders and the resuscitation techniques, and the second theme was subjective perceptions based on caregivers' beliefs and emotional responses. Caregivers need counseling, preparation, and education on Do Not Resuscitate orders and resuscitation methods to improve Do Not Resuscitate discussions. Healthcare providers need to be empowered to hold those discussions.

Land use drives differential resource selection by African elephants in the Greater Mara Ecosystem, Kenya

Understanding drivers of space use by African elephants is critical to their conservation and management, particularly given their large home-ranges, extensive resource requirements, ecological role as ecosystem engineers, involvement in human-elephant conflict and as a target species for ivory poaching. In this study we investigated resource selection by elephants inhabiting the Greater Mara Ecosystem in Southwestern Kenya in relation to three distinct but spatially contiguous management zones: (i) the government protected Maasai Mara National Reserve (ii) community-owned wildlife conservancies, and (iii) elephant range outside any formal wildlife protected area. We combined GPS tracking data from 49 elephants with spatial covariate information to compare elephant selection across these management zones using a hierarchical Bayesian framework, providing insight regarding how human activities structure elephant spatial behavior. We also contrasted differences in selection by zone across several data strata: sex, season and time-of-day. Our results showed that the strongest selection by elephants was for closed-canopy forest and the strongest avoidance was for open-cover, but that selection behavior varied significantly by management zone and selection for cover was accentuated in human-dominated areas. When contrasting selection parameters according to strata, variability in selection parameter values reduced along a protection gradient whereby elephants tended to behave more similarly (limited plasticity) in the human dominated, unprotected zone and more variably (greater plasticity) in the protected reserve. However, avoidance of slope was consistent across all zones. Differences in selection behavior was greatest between sexes, followed by time-of-day, then management zone and finally season (where seasonal selection showed the least differentiation of the contrasts assessed). By contrasting selection coefficients across strata, our analysis quantifies behavioural switching related to human presence and impact displayed by a cognitively advanced megaherbivore. Our study broadens the knowledge base about the movement ecology of African elephants and builds our capacity for both management and conservation.

Links between Household-Level Income-Generating Agricultural Intervention and the Psychological Well-Being of Adolescent Girls in Human Immunodeficiency Virus-Affected Households in Southwestern Kenya: A Qualitative Inquiry

BackgroundAdolescent girls may experience poor psychological well-being, such as social isolation, shame, anxiety, hopelessness, and despair linked to food insecurity. ObjectivesThis study aimed to investigate the experiences with and perceived effects of a household-level income-generating agricultural intervention on the psychological well-being of adolescent girls in human immunodeficiency virus (HIV)-affected households in southwestern Kenya. MethodsWe conducted 62 in-depth interviews with HIV-affected adolescent girls and caregiver dyads in Adolescent Shamba Maisha (NCT03741634), a sub-study of adolescent girls and caregivers with a household member participating in Shamba Maisha (NCT01548599), a multisectoral agricultural and finance intervention trial aimed to improve food security and HIV health indicators. Participants were purposively sampled to ensure diversity in terms of age and location. Data were audiotaped, transcribed, translated, and uploaded into Dedoose (Sociocultural Research Consultants, LLC) software for management. Data were analyzed thematically based on reports from Dedoose. ResultsWe found evidence that a household-level structural intervention aimed at increasing food and financial security among persons living with HIV can contribute to better psychological well-being among adolescent girls residing in these households. The intervention also affected: 1) reduction of social isolation, 2) reduction of shame and stigma, 3) increased attendance and concentration in school, 4) improved caregiver mental health, and 5) reduced parental aggression and improved household communication. These associations were reported more commonly among those in the intervention arm than the control arm. ConclusionsThis study extends existing research by demonstrating how multisectoral structural interventions delivered at a household level can improve the psychological well-being of adolescents. We recommend that future research test livelihood interventions designed specifically for adolescent girls that integrate food-security interventions with other elements to address the social and psychological consequences of food insecurity holistically.This trial was registered at clinicaltrials.gov as NCT03741634.

A multi-component forager-to-herder sequence at Kapsoo Rockshelter (Chebinyiny) on the Uasin Gishu Plateau, Kenya

ABSTRACT Early archaeological investigations of the Uasin Gishu Plateau of west-central Kenya highlighted its potential importance as a geographic link between the Central Rift, the Lake Victoria Basin and the western Kenyan highlands from the Middle Stone Age to the Pastoral Neolithic and Iron Age periods. Despite this, the region has seen little focused research in recent decades. Here, we report on archaeological research at the site of Kapsoo Rockshelter, Chebinyiny Hill (GmJf1), near Moiben on the northern end of the Uasin Gishu Plateau. We combine data from original fieldwork in 1982 with renewed test excavations at Kapsoo carried out in 2022. Results of lithic, faunal and material analyses combined with radiocarbon dating reflect occupation of the northern plateau from the late Pleistocene to the late Holocene and provide the first radiometric dates for the presence of mobile herders in this region. Quantitative assessment of the rich lithic record at the site identifies significant technological change over time, providing a description of the regional Later Stone Age sequence. Finally, we present hypotheses for the relationship between Kapsoo and the nearby undated sequence at Muringa, as well as how these records fit into broader patterns of economic change across southwestern Kenya.

Re-imagining Moralities and Masculinities in the Context of Male Circumcision and Marriage in South-Western Kenya Communities

Male circumcision and marriage are significant cultural practices that mark a critical stage in a person’s life in many African societies. Many rites of passage that exist, circumcision and marriage are intertwined. Marriage and circumcision like other cultural themes have attracted wide scholarship of diverse depths. Such studies have underlined, among others, the nature, forms, types and even the place of the two initiation rites in society. What, however, has not received adequate attention are the (re)negotiations and contestations between morality and masculinities in the context of male circumcision and marriage. Based on this understanding, and drawing on the findings of a qualitative study conducted in Kisii, and Homa Bay counties in South-Western Kenya, the researchers in this paper argue that circumcision and initiation determine ones’s masculinity, which as well acts as a gateway to marriage and social wellbeing. Combined, the two cultural practices can be presented as central morality and cultural notions that inform, underpin, and serve as the dominant paradigms that direct the lives and activities of African people from birth to death. The study suggests that despite its significance over decades, the practice of circumcision has changed over time in relation to the importance people attach to it, how it is practiced, and the attention it attracts from the society. The study recommends the need for a critical interrogation of morality in relation to what fits individuals and the contemporary world as they practice the rites of passage, while at the same time maintaining what is morally acceptable in the society.

Women participation in artisanal gold mining: A case of macalder mines, Kenya

The aim of the study is to determine women's participation in artisanal gold mining in Macalder mines in Migori County, which is found in Southwestern Kenya. A mixed-method research design was used in the study to collect and analyse both qualitative and quantitative data. Purposive and snowball non-probability sampling methods were used to select the sample size since there is no proper data on the number of women involved in artisanal gold mining. A questionnaire survey and focus group discussions were used to collect data from the respondents. Descriptive statistics and inferential statistics were used to analyse the data. The results indicate that 80.8 per cent of the respondents were between 18 and 49 years old. Most of the women working in Macalder gold mines were earning <Kshs 5,000, which was not enough to sustain them. Low remuneration, health risks, cultural bias, domestic responsibilities and sexual harassment were the main challenges that women were facing in the gold mines. The study recommends formulating and implementing policies that recognise and safeguard the interests of women in artisanal gold mining. Women should also be provided with protective gear to reduce the health risks from gold processing.

Dissipation of environmental DDT and its metabolites in selected rivers of south-western Kenya

Rivers are the main source of domestic and industrial water supplies in Kenya. In this study, water sampling was done at eight upstream sites in the Kuja River catchment, Kenya, between October 2016 and April 2017, specifically to test for residue levels of the organochlorine pesticide, dichlorodiphenyltrichloroethane (p,p′-DDT), and its degradation products, dichlorodiphenyldichloroethylene (p,p′-DDE) and dichlorodiphenyldichloroethane (p,p′-DDD). The analysis was performed with gas chromatography coupled with electron-capture detection in an autosampler. Concentrations of these residues in the surface water samples ranged from below detection limit (BDL) to 1.1113 μg l-1 in the wet season, and from BDL to 2.007 μg l-1 in the dry season. Overall, the highest mean residue concentration was for p,p′-DDT (0.9669 ± 0.2994 μg l-1). The lowest mean concentration in the dry season was that of p,p′-DDE (0.1824 ± 0.0964 μg l-1), and in the wet season it was p,p′-DDD (0.0610 ± 0.0038 μg l-1). From our results it is evident that DDT is still in use and detectable in the study area, though the mean levels detected were below the acceptable thresholds for natural water as suggested by the World Health Organization (WHO). Except for a slightly higher mean spatial distribution of the metabolite p,p′-DDT, the resides were recorded as below the maximum admissible concentrations of pesticide residues in drinking water, as set out in WHO guidelines and by Kenya’s National Environment Management Authority (NEMA). Monitoring organochlorine pesticide contamination levels in Kenya’s water resources should be scaled up to leverage potential for a sustainable blue economy and in safeguarding human and environmental health.

Expanded geographic distribution and dietary strategies of the earliest Oldowan hominins and Paranthropus

The oldest Oldowan tool sites, from around 2.6 million years ago, have previously been confined to Ethiopia's Afar Triangle. We describe sites at Nyayanga, Kenya, dated to 3.032 to 2.581 million years ago and expand this distribution by over 1300 kilometers. Furthermore, we found two hippopotamid butchery sites associated with mosaic vegetation and a C4 grazer-dominated fauna. Tool flaking proficiency was comparable with that of younger Oldowan assemblages, but pounding activities were more common. Tool use-wear and bone damage indicate plant and animal tissue processing. Paranthropus sp. teeth, the first from southwestern Kenya, possessed carbon isotopic values indicative of a diet rich in C4 foods. We argue that the earliest Oldowan was more widespread than previously known, used to process diverse foods including megafauna, and associated with Paranthropus from its onset.

Cost of Home-Based Couples Human Immunodeficiency Virus Counseling and Testing and Human Immunodeficiency Virus Self-Testing During Pregnancy and Postpartum in Southwestern Kenya

ObjectiveHome-based couples HIV testing and counseling and HIV self-testing (HIVST) for pregnant women can promote HIV status disclosure and male partner testing; however, cost data are lacking. We examined a home-based couples intervention (HBCI) and HIVST intervention costs per couple (unit cost) during pregnancy and postpartum in Kenya. MethodsThis randomized controlled trial is comparing HBCI and HIVST for couples among pregnant women attending antenatal care clinics in two counties in southwestern Kenya. We used micro-costing to estimate the unit cost per couple receiving the intervention as the total of direct and indirect costs for each study arm in 2019 US$. We used a one-month window to conduct a time and motion study to determine personnel effort and resources. We then compared the unit cost by arm, identified key cost drivers, and conducted sensitivity analyses for cost uncertainties. ResultsAt base-case, the unit cost was $129.01 and $41.99, respectively, for HBCI and HIVST. Personnel comprised half of the unit cost for both arms. Staff spent more time on activities related to participant engagement in HBCI (accounting for 6.4% of the unit cost) than in HIVST (2.3%). Staff training was another key cost driver in HBCI (20.1% of the unit cost compared to 12.5% in HIVST). Sensitivity analyses revealed that the unit cost ranges were $104.64-$154.54 for HBCI and $30.49-$56.59 for HIVST. ConclusionsOur findings may guide spending decisions for future HIV prevention and treatment programs for pregnant couples in resource-limited settings such as Kenya.

Adherence level to the World health Organization surgical safety checklist and associated factors in operating rooms in a county teaching and referral hospital in South-Western Kenya

IntroductionWorld Health Organization (WHO) introduced the Surgical Safety Checklist (SSC) in 2008 to assure safety in Operating Rooms (ORs). Globally, adherence level varies with developing countries having the lowest levels compared to their developed counterparts. ObjectivesWe aimed to assess the level of adherence and identify factors that influence adherence to the WHO SSC in a county teaching and referral hospital in South Western Kenya. MethodsThe study preferred a mixed method cross-sectional design. WHO SSC adherence level was assessed by conducting a retrospective review of 424 surgical files that were randomly selected. OR staff (40) were targeted using census method and 31of them were interviewed using structured interviews to determine qualitative data on staff and institutional factors that influenced adherence practices to the WHO SSC. Quantitative data was analysed using Statistical Package for Social Sciences (SPSS). Both descriptive and analytical statistics were generated with statistical significance set at p=<0.05. Content analysis was adopted for qualitative data. Study FindingsWHO SSC was present in 100 % of the post-surgical files. Filling of the WHO SSC was attempted in 7.1 % of the surgeries done. Better adherence was noted in elective surgeries (78 %) compared to emergency (22%) ones. There was a varying adherence level per the type of surgeries with adult general surgeries posting better adherence (40 %) and orthopaedic surgeries were least adherent at 4 %. A decreasing trend in adherence level was noted subsequently through the three moments of surgery (‘sign in’ (5.7 %); ‘Time out’ (1.8 %); and ‘Sign out’ (0.7 %)). The average adherence level was established to be 2.7 %. Key staff factors that influenced adherence practices included; years of experience (p = 0.004), attitude (p = 0.014) and perception(p = 0.009). Additionally, institutional factors were; lack of essential consumables and drugs (77.4 %), lack of refresher trainings (54.5 %), understaffing (48.4 %) and lack of management support (45.2 %). ConclusionThe level of adherence to the WHO SSC was critically low. The aforementioned staff and institutional factors influenced the level of adherence. The study recommends a structured health system-based quality improvement intervention to improve the outcome (adherence) by addressing the root causes (Staff and institutional) to promote patient safety in OR.

Accelerating agricultural expansion in the greater Mau Forest Complex, Kenya

Land use land cover (LULC) change can modify local, regional, and global socio-environmental systems. Globally, much LULC change study is aimed at deforestation and urbanization with less focus on other LULC categories like grasslands and savannas. Here, we focus our LULC change study on southwestern Kenya, a topographically varying region of forest, grassland, and cropland. This study leveraged existing remotely sensed Landsat landcover data to evaluate uncertainty of LULC classification, quantify the nature and magnitude of changes, and illustrate the role of topography (slope and elevation) in determining the spatiotemporal characteristics of the landscape between 1990 and 2018. We also analyzed landscape metrics of fragmentation and dominance at the class-level and employed Kruskal-Wallis tests to examine the characteristics and statistical significance of the changes. We obtained an overall classification accuracy of 86% and establish that over the 28-year period, cropland increased by 22.5% and became less fragmented, forest decreased by 6.6% and became less fragmented, while grassland decreased by 16% and became more fragmented. Results showed statistical difference (p < 0.05) in LULC change among different topographic classes. Our results indicate that deforestation in the region has slowed in the past decades, likely due to conservation efforts, but conversion of grassland to cropland has accelerated. Grasslands, including pastures, provide essential ecosystem services both to people and the environment and should not be overlooked in conservation initiatives.

The Translation of יהוה: Part 2, The Case of Dholuo

The first part of this study ( TBT 72[1]: 50–60) reviewed the history of the rendering of the key term יהוה YHWH in Bible translations into Dholuo (spoken in southwestern Kenya and northwestern Tanzania). This second part considers the translation of this key term within the context of modern Dholuo language and culture. The different renderings in two existing translations are analyzed and put in the broader perspective of Bible translation in Africa. Finally, the paper proposes a rendering for יהוה that does justice to the Dholuo culture and tradition, while maintaining the specific characteristics that are present in the source text.

Transcriptome profile of spleen tissues from locally-adapted Kenyan pigs (Sus scrofa) experimentally infected with three varying doses of a highly virulent African swine fever virus genotype IX isolate: Ken12/busia.1 (ken-1033)

BackgroundAfrican swine fever (ASF) is a lethal hemorrhagic disease affecting domestic pigs resulting in up to 100% mortality rates caused by the ASF virus (ASFV). The locally-adapted pigs in South-western Kenya have been reported to be resilient to disease and harsh climatic conditions and tolerate ASF; however, the mechanisms by which this tolerance is sustained remain largely unknown. We evaluated the gene expression patterns in spleen tissues of these locally-adapted pigs in response to varying infective doses of ASFV to elucidate the virus-host interaction dynamics.MethodsLocally adapted pigs (n = 14) were experimentally infected with a high dose (1x106HAD50), medium dose (1x104HAD50), and low dose (1x102HAD50) of the highly virulent genotype IX ASFV Ken12/busia.1 (Ken-1033) isolate diluted in PBS and followed through the course of infection for 29 days. The in vivo pig host and ASFV pathogen gene expression in spleen tissues from 10 pigs (including three from each infective group and one uninfected control) were analyzed in a dual-RNASeq fashion. We compared gene expression between three varying doses in the host and pathogen by contrasting experiment groups against the naïve control.ResultsA total of 4954 differentially expressed genes (DEGs) were detected after ASFV Ken12/1 infection, including 3055, 1771, and 128 DEGs in the high, medium, and low doses, respectively. Gene ontology and KEGG pathway analysis showed that the DEGs were enriched for genes involved in the innate immune response, inflammatory response, autophagy, and apoptosis in lethal dose groups. The surviving low dose group suppressed genes in pathways of physiopathological importance. We found a strong association between severe ASF pathogenesis in the high and medium dose groups with upregulation of proinflammatory cytokines and immunomodulation of cytokine expression possibly induced by overproduction of prostaglandin E synthase (4-fold; p < 0.05) or through downregulation of expression of M1-activating receptors, signal transductors, and transcription factors. The host-pathogen interaction resulted in induction of expression of immune-suppressive cytokines (IL-27), inactivation of autophagy and apoptosis through up-regulation of NUPR1 [5.7-fold (high dose) and 5.1-fold (medium dose) [p < 0.05] and IL7R expression. We detected repression of genes involved in MHC class II antigen processing and presentation, such as cathepsins, SLA-DQB1, SLA-DOB, SLA-DMB, SLA-DRA, and SLA-DQA in the medium and high dose groups. Additionally, the host-pathogen interaction activated the CD8+ cytotoxicity and neutrophil machinery by increasing the expression of neutrophils/CD8+ T effector cell-recruiting chemokines (CCL2, CXCL2, CXCL10, CCL23, CCL4, CXCL8, and CXCL13) in the lethal high and medium dose groups. The recovered pigs infected with ASFV at a low dose significantly repressed the expression of CXCL10, averting induction of T lymphocyte apoptosis and FUNDC1 that suppressed neutrophilia.ConclusionsWe provide the first in vivo gene expression profile data from locally-adapted pigs from south-western Kenya following experimental infection with a highly virulent ASFV genotype IX isolate at varying doses that mimic acute and mild disease. Our study showed that the locally-adapted pigs induced the expression of genes associated with tolerance to infection and repression of genes involved in inflammation at varying levels depending upon the ASFV dose administered.

Linking farmers’ risk attitudes, livelihood diversification and adoption of climate smart agriculture technologies in the Nyando basin, South-Western Kenya

Climate smart agriculture (CSA) technologies are innovations meant to reduce the risks in agricultural production among smallholder farmers. Among the factors that influence farmer adoption of agricultural technologies are farmers' risk attitudes and household livelihood diversification. This study, focused on determining how farmers' risk attitudes and household livelihood diversification influenced the adoption of CSA technologies in the Nyando basin. The study utilized primary data from 122 households from two administrative regions of Kisumu and Kericho counties in Kenya. The study employed the multivariate probit (MVP) and ordered probit (OP) models and descriptive statistics in data analysis using Stata 14.0. Results from the study indicated that farmers’ risk attitudes had a significant negative influence in the adoption of terraces, ridges and bunds as well as the intensity of adoption of given CSA technologies. Household livelihood diversification had a significant negative influence in the adoption of stress tolerant livestock but did not have a significant effect on the intensity of adoption of given CSA technologies. The study recommends that relevant stakeholders should introduce an appropriate agricultural index insurance product to Nyando basin farmers to encourage the broader adoption of CSA technologies.

Prevalence of esophageal squamous dysplasia in relatives of patients with esophageal cancer in Southwestern Kenya

BackgroundEsophageal squamous cell carcinoma (ESCC) and its asymptomatic precursor lesion, esophageal squamous dysplasia (ESD), are common in East Africa. It is unknown whether family history of esophageal cancer is a risk factor for both ESD and ESCC in Africa, and whether family members of affected persons should be screened. MethodsWe recruited 296 asymptomatic adult first-degree relatives of ESCC patients residing in southwestern Kenya. Participants completed questionnaires and underwent endoscopy with Lugol’s iodine staining and biopsy to determine the prevalence of ESD. Prevalence comparisons were made with a prior population-based cohort from the same catchment area who also underwent Lugol’s chromoendoscopy. ResultsMean age was 40.7 years, compared to 62.7 years in the prior population study. The overall prevalence of ESD/ESCC among first-degree relatives was 14.7%, comparable to the background population prevalence of 14.4%, and this comparability remained even after adjusting for the different age distributions of the studies. Post-primary education was the only measured variable that was associated with a decreased risk of ESD/ESCC (adjusted OR=0.31, 95% CI: 0.11, 0.83). There was heterogeneity in the ESD prevalence across families, even after adjustments for varying age and other measured factors. ConclusionsThe prevalence of esophageal squamous dysplasia among first-degree relatives of persons with ESCC was similar to that of the background population of southwestern Kenya; however, there was heterogeneity in ESD prevalence between families, suggesting other genetic or environmental factors may influence family prevalence. Further study of families with a high prevalence of ESD or ESCC is justified.