South-western Australia Research Articles

O062 Adolescents’ Mental Health Hrajectories throughout the COVID-19 Pandemic and the Protective role of Healthy Sleep Patterns

Abstract Introduction The COVID-19 pandemic has seen a rise in emotional problems among adolescents. Improving young people's mental health is a public health priority, and examining protective factors throughout the pandemic can offer insights on targets for prevention. This study aimed to investigate longitudinal trajectories of depressive and anxiety symptoms among a large sample of Australian adolescents and to examine sleep patterns in high- and low-risk trajectories. Methods This study utilises data from the control group of the “Health4Life” cluster RCT. We used three waves of questionnaire data collected annually (from 2019) from Year 7 students at 71 secondary schools across New South Wales, Queensland and Western Australia (N = 2,781, Mage = 12.6, SD = .51; 47% boys and 1.4% ‘prefer not to say’). Adolescents reported on their mental health (i.e., depressive and anxiety symptoms) and sleep (i.e., sleepiness, weekday sleep duration, SOL, WASO, and chronotype). Results We found four trajectories of depressive symptoms: low-stable (64.3%), average-increasing (19.2%), high-decreasing (7.1%), moderate-increasing (9.4%), and three anxiety symptom trajectories: low-stable (74.8%), average-increasing (11.6%), high-decreasing (13.6%). Adolescents in low-risk trajectories were more likely to be boys and to report shorter SOL and WASO, longer sleep duration, less sleepiness, and earlier chronotype. Where mental health waxed and waned, sleep patterns changed in the same direction. Discussion This is the first study to thoroughly examine adolescents’ sleep patterns as a protective factor for the development of emotional problems during the pandemic. Healthy sleep was clearly linked to low-risk trajectories, although this association is likely bi-directional.

Understanding the complex dynamics of climate change in south-west Australia using Machine Learning

Understanding the complex dynamics of climate change in south-west Australia using Machine Learning

Calcifuge and soil-indifferent Proteaceae from south-western Australia: novel strategies in a calcareous habitat

Background and aimsProteaceae are a prominent plant family in south-western Australia. Most Proteaceae are ‘calcifuge’, occurring exclusively on old phosphorus (P)-impoverished acidic soils, with a few ‘soil-indifferent’ species also found on young P-richer calcareous soils. Calcium (Ca)-enhanced P toxicity explains the calcifuge habit of Proteaceae. However, previous research has so far been focused exclusively on the roles of Ca and P in determining Proteaceae distribution, and consequently there is little knowledge on how other soil-based strategies influence this distribution. We aimed to study the effects of young calcareous soils on four soil-grown Proteaceae and assess differences between calcifuge and soil-indifferent Proteaceae to better understand their natural distribution.MethodsTwo calcifuge and two soil-indifferent Proteaceae from south-western Australia were grown in six contrasting soils, including young calcareous, and old acidic soils.ResultsWhen grown in calcareous soils all species showed root growth inhibition, micronutrient deficiency, Ca-enhanced P toxicity, and negative impacts on physiology. Calcifuge species were more sensitive to calcareous soils than soil-indifferent ones, although this varied between genera. Soil-indifferent species tended to produce more cluster roots, release more carboxylates per root mass, and allocate less Ca to their leaves, compared with calcifuges; they also had smaller seeds and were less sensitive to Ca-enhanced P toxicity.ConclusionWe surmise that a combination of these traits allows soil-indifferent species to tolerate calcareous soils. This study provides insight into how Proteaceae respond to young calcareous soils and how this influences their distribution.

A preliminary phylogeny for the pseudoscorpion family Garypinidae (Pseudoscorpiones: Garypinoidea), with new taxa and remarks on the Australasian fauna

The pseudoscorpion family Garypinidae is globally distributed with 79 species in 21 genera and several species represented by Mesozoic and Eocene fossils. This was recently included with the family Larcidae in a unique superfamily, Garypinoidea but there are no phylogenetic hypotheses for the group. Sequence data were obtained for 14 species in 8 genera and numerous outgroup taxa that formed the basis for a preliminary molecular phylogeny. A new subfamily classification is proposed with Protogarypininae, subfamily nov. comprising five genera mostly found in the southern hemisphere, Amblyolpiinae subfamily nov. comprising two genera and Garypininae for the remaining genera. Several new taxa are described including the first Australian species of Aldabrinus, A. rixi sp. nov., a new genus from South-East Asia, Nobilipinus, comprising Nobilipinus nobilis (With, 1906), N. vachoni (Redikorzev, 1938) (that is removed from the synonymy of G. nobilis) and five new species, N. affinis, N. galeatus, N. karenae, N. kohi and N. tricosus, and Solinus pingrup sp. nov. from south-western Australia. Paraldabrinus Beier, 1966 is newly synonymised with Aldabrinus, and Indogarypinus Murthy and Ananthakrishan, 1977 is newly synonymised with Solinus. The holotype of Garypinus mirabilis With, 1907 from Hawaii is redescribed but found to be a tritonymph, rendering the generic identity uncertain. ZooBank: urn:lsid:zoobank.org:pub:E15E4705-0697-4208-9338-A778343996CA

Banksia (Proteaceae) contains less phylogenetic diversity than expected in Southwestern Australia

AbstractBanksia L.f., an iconic genus of the Proteaceae, is endemic to Australia, with its highest species richness and phylogenetic diversity (PD) in southwestern Western Australia. Analysis of the phylogenetic component of richness and endemism uncovered important patterns of Banksia evolutionary history that are not seen at the species level. We found that Banksia is significantly phylogenetically clustered in this region, likely due to recent evolutionary radiations. We also found significant concentrations of phylogenetic endemism in this region, both neoendemism (short, range‐restricted evolutionary branches) and paleoendemism (long, range‐restricted evolutionary branches). There is a striking northwest to southeast divide in phyloturnover in southwestern Western Australia. The majority of the variation in turnover patterns can be explained by environmental factors, with climate representing the largest covariate. This study adds to the growing body of evidence that indicates the importance of integrating phylogenetic and biodiversity data to inform conservation planning.

Assessing the impact of habitat and captivity status on volatilome profiles of the illegally traded shingleback, Tiliqua rugosa

Volatilomics is the study of the total biogenic volatile organic compounds (BVOCs) produced by an organism. This field has been used to assess organism and ecosystem health, as well as determine BVOC biomarkers for forensic purposes, including the detection of human remains, ignitable liquid residues and illicit drugs. For volatilomics to be applied in wildlife-victim casework (e.g. the illegal wildlife trade) a large reference database must be collected across each targeted species range. Adequate sample sizes must be collected from different habitats spanning across the species range of the targeted species to evaluate volatilome variability associated with different environmental and dietary characteristics. This will allow for assessment of chemical diversity and the determination of BVOC biomarkers that are relevant to wildlife forensic cases (e.g. detection, species identification, geographic origin assessment). This study collected the first live animal volatilome database, using the highly trafficked and widely distributed Australian shingleback lizard (Tiliqua rugosa). Optimised thermal desorption and analysis methods were used to examine 127 wild shingleback volatilome samples collected from sites across New South Wales, South Australia and Western Australia and 28 volatilome samples from captive shinglebacks. The results demonstrated that volatilome profiles and chemical diversity differed across each sampling region, potentially related to habitat and diet changes. At least 7 volatilome samples were required to capture chemical diversity in a sampling region. Forty-four tentatively identified BVOCs were shared across all sampling regions and captive animals which may aid in detection purposes. Bioregion-specific BVOCs were also identified, which will also aid in geographic assignment of confiscated individuals. This work demonstrates the importance of sample sizes in capturing chemical diversity within Bioregions prior to downstream volatilome analysis for the establishment of wildlife forensic databases and biomarker selection.

HOW BEACH STATE INFLUENCES WAVE RUNUP ON A PERCHED BEACH IN SOUTHWESTERN AUSTRALIA

Approximately 20 to 30 percent of the world’s coastlines are fronted by shallowly buried or outcropping shore platforms overlain by perched beaches (Kirk, 1977; Marshall and Stephenson, 2011; Trenhaile, 2002). Seasonal erosion of perched sediment can shift the beach state from ‘accreted’ to ‘exposed’ (Gallop et al., 2011), and the effect this has on wave-induced flood risk (known as wave runup) is unknown. As sea levels rise and storm severity increases, understanding how beach state influences wave runup is crucial for minimising coastal hazard risk and managing perched beach coastlines. In this work, idealised numerical modelling and field observations along a perched beach in southwestern Australia were used to quantify the influence of beach state on wave runup, setup, and swash processes.

Leaf phosphorus allocation to chemical fractions and its seasonal variation in south-western Australia is a species-dependent trait

South-western Australia is a global biodiversity hotspot and has some of the oldest and most phosphorus (P)-impoverished soils in the world. Proteaceae is one of the dominant P-efficient plant families there, but it is unknown how leaf P concentrations and foliar P allocation of Proteaceae and coexisting dominant plant families vary between seasons and habitats. To investigate this, we selected 18 species from Proteaceae, Myrtaceae and Fabaceae, six from each family, in two habitats from Alison Baird Reserve (32°1′19′′S 15°58′52′′E) in Western Australia. Total leaf P and nitrogen (N) concentrations, leaf mass per area, photosynthetic rate, pre-dawn leaf water potential and foliar P fractions were determined for each species both at the end of summer (March 2019 and early April 2020) and at the end of winter (September 2019). Soil P availability was also determined for each site. This is the very first study that focused on seasonal changes of foliar P fractions from different P-impoverished environments in three plant families. However, contrary to our expectation, we found little evidence for convergence of foliar P allocation within family, season or habitat. Each species exhibited a specific species-dependent pattern of foliar P allocation, and many species showed differences between seasons. Native plants in south-western Australia converged on a high photosynthetic P-use efficiency, but each species showed its own unique way associated with that outcome.

Incorporating the pedigree information in multi-environment trial analyses for improving common vetch.

Common vetch is one of the most profitable forage legumes due to its versatility in end-use which includes grain, hay, green manure, and silage. Furthermore, common vetch is one of the best crops to rotate with cereals as it can increase soil fertility which results in higher yield in cereal crops. The National Vetch Breeding Program located in South Australia is focused on developing new vetch varieties with higher grain and dry matter yields, better resistance to major diseases, and wider adaptability to Australian cropping environments. As part of this program, a study was conducted with 35 field trials from 2015 to 2021 in South Australia, Western Australia, Victoria, and New South Wales with the objective of determining the best parents for future crosses and the vetch lines with highest commercial value in terms of grain yield production. A total of 392 varieties were evaluated. The individual field trials were combined in a multi-environment trial data, where each trial is identified as an environment. Multiplicative mixed models were used to analyze the data and a factor analytic approach to model the genetic by environment interaction effects. The pedigree of the lines was then assembled and incorporated into the analysis. This approach allowed to partition the total effects into additive and non-additive components. The total and additive genetic effects were inspected across and within environments for broad and specific selections of the lines with the best commercial value and the best parents. Summary measures of overall performance and stability were used to aid with selection of parents. To the best of our knowledge, this is the first study which used the pedigree information to breed common vetch. In this paper, the application of this statistical methodology has been successfully implemented with the inclusion of the pedigree improving the fit of the models to the data with most of the total genetic variation explained by the additive heritable component. The results of this study have shown the importance of including the pedigree information for common vetch breeding programs and have improved the ability of breeders to select superior commercial lines and parents.

Quantifying the impacts of an invasive weed on habitat quality and prey availability for tiger snakes (Notechis scutatus) in urban wetlands

Invasive plants are a threat to natural ecosystems worldwide, with urban wetlands being some of the most susceptible and highly modified environments of all. The tiger snake (Notechis scutatus) is a top predator that persists in urban wetlands of south-western Australia, many of which have been degraded by introduced kikuyu grass (Cenchrus clandestinus). To evaluate the potential impact of kikuyu grass on habitat quality for tiger snakes we quantified the structural features of habitats within wetlands degraded by kikuyu grass and compared them to wetlands with native vegetation. We also examined tiger snake prey availability, assessed predation risk for juvenile snakes using clay models, and measured the thermal quality of the vegetation. Proliferation of kikuyu grass has reduced habitat structural heterogeneity by reducing available bare ground and increasing vegetation density. This homogenisation of habitat structure had little effect on the predation risk for juveniles or the thermal properties of tiger snake shelter sites; however, one key prey species, the motorbike frog, had significantly lower abundance in the most impacted habitat. Habitat types with more structural complexity also offered tiger snakes more stable thermal regimes and lower predation risk. These findings indicate that the current extent of kikuyu grass invasion offers overall similar habitat quality for tiger snakes to native vegetation and may contribute to their persistence in urban wetlands; however, both tiger snakes and their anuran prey may benefit from increased habitat structural complexity.

Mitochondrial genomic investigation reveals a clear association between species and genotypes of Lucilia and geographic origin in Australia

BackgroundLucilia cuprina and L. sericata (family Calliphoridae) are globally significant ectoparasites of sheep. Current literature suggests that only one of these blowfly subspecies, L. cuprina dorsalis, is a primary parasite causing myiasis (flystrike) in sheep in Australia. These species and subspecies are difficult to distinguish using morphological features. Hence, being able to accurately identify blowflies is critical for diagnosis and for understanding their relationships with their hosts and environment.MethodsIn this study, adult blowflies (5 pools of 17 flies; n = 85) were collected from five locations in different states [New South Wales (NSW), Queensland (QLD), Tasmania (TAS), Victoria (VIC) and Western Australia (WA)] of Australia and their mitochondrial (mt) genomes were assembled.ResultsEach mt genome assembled was ~ 15 kb in size and encoded 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and a control region. The Lucilia species mt genomes were conserved in structure, and the genes retained the same order and direction. The overall nucleotide composition was heavily biased towards As and Ts—77.7% of the whole genomes. Pairwise nucleotide diversity suggested divergence between Lucilia cuprina cuprina, L. c. dorsalis and L. sericata. Comparative analyses of these mt genomes with published data demonstrated that the blowflies collected from sheep farm in TAS clustered within a clade with L. sericata. The flies collected from an urban location in QLD were more closely related to L. sericata and represented the subspecies L. c. cuprina, whereas the flies collected from sheep farms in NSW, VIC and WA represented the subspecies L. c. dorsalis.ConclusionsPhylogenetic analyses of the mt genomes representing Lucilia from the five geographic locations in Australia supported the previously demonstrated paraphyly of L. cuprina with respect to L. sericata and revealed that L. c. cuprina is distinct from L. c. dorsalis and that L. c. cuprina is more closely related to L. sericata than L. c. dorsalis. The mt genomes reported here provide an important molecular resource to develop tools for species- and subspecies-level identification of Lucilia from different geographical regions across Australia.Graphical

Atlantic Multidecadal Variability modulates the climate impacts of El Niño–Southern Oscillation in Australia

Atlantic Multidecadal Variability (AMV) modulates El Niño–Southern Oscillation (ENSO) dynamics. Here, we explore the effect of warm (AMV+) and cold (AMV−) AMV conditions on the austral summer teleconnection of ENSO to Australia using idealized simulations performed with the NCAR-CESM1 model. AMV+ strengthens the mean and extreme precipitation and temperature responses to El Niño in south-western Australia and weakens the mean precipitation and temperature impacts in north-eastern Australia. The modulation of La Niña impacts by AMV is asymmetric to El Niño, with a weakening of the mean and extreme precipitation and temperature responses in eastern Australia. Decomposing the total difference in ENSO response between AMV phases, we find that the signals are mainly explained by the direct AMV modulation of ENSO and its teleconnections rather than by changes in background climate induced by AMV. The exception is ENSO-driven fire impacts, where there is a significant increase in burned area in south-eastern Australia only when El Niño and AMV+ co-occur. However, modulation of ENSO between AMV+ and AMV− does offset ∼37% of the decrease in burned area extent during La Niña summers. The altered surface climate response to ENSO in Australia by AMV is attributed to variations in large-scale atmospheric circulation. Under AMV+, there is increased subsidence over western Australia during El Niño associated with a westward shift of the local Walker circulation. A weakening of the upwelling branch of the local Hadley circulation over north-eastern Australia is responsible for the weakening of La Niña impacts in AMV+, accompanied by a strengthening of subsidence in south central Australia due to a weakening of the local Hadley circulation, amplifying La Niña impacts over this region. The results suggest the potential for AMV to drive multidecadal variability in ENSO impacts over Australia.

Cardiac rehabilitation patient data management and quality improvement in Australia: A national survey

Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main funding source(s): National Health and Medical Research Council Synergy Grant Background Receipt of guideline-indicated comprehensive cardiac rehabilitation improves patient outcomes. Optimum service delivery and meeting quality standards necessitates accurate standardised data collection and reporting. The methods, processes and systems used for data capture and reporting in cardiac rehabilitation are under-documented, limiting identification of areas needing change and creation of a platform for quality improvement (QI) at national levels. Purpose To describe data capture, management, and reporting and the awareness and use of quality indicators in contemporary Australian CR programs. Methods CR programs (n=396) were identified through professional and national directories, jurisdictional networks, webinars, and direct provider contact. Program coordinators completed an online survey on individual service characteristics, data capture and management systems (paper, electronic, database, integrated platform) including awareness, use and usefulness of the published National Cardiac Rehabilitation Quality toolkit components. Results Respondents totalled 319 (80.5%), from all eight states and territories. Coverage of Metropolitan, rural and remote towns, and regional centres was 46.3%, 45.9% and 7.8%, respectively. Indicative annual patient enrolments were >200 (31.0%), 51-200 (46.4%) and ≤50 (22.6%). Most programs used ≥2 data management systems: paper and electronic 62.6% or multiple e-systems 19.1%, or one system, either paper 20.9% or electronic 16.3%. Use of multiple systems and combinations of systems did not differ by number of patients enrolled. QI systems were infrequently used: QI Platforms 17.2%, QI Databases 10.0%. Supplementary to patient care, data gained was used for reports for management (57.0%), QI (56.1%), funding support (43.2%), required jurisdictional reporting (41.1%) and research (30.7%). Higher volume programs were more likely to be using the data capture for multiple purposes. Independent covariates of services using data for QI were enrolments of >200 (OR 3.83 95% CI 1.76, 8.34) and the state/territory location, such that use of QI was less likely in the states of Victoria (OR 0.24 95% CI 0.08, 0.77), New South Wales (OR 0.25 95% CI 0.08, 0.76) and Western Australia (OR 0.16 95% CI 0.05, 0.57). Conclusions Management of cardiac rehabilitation patient data is highly variable across Australian programs and jurisdictions, often including multiple different systems, with limited use of the data for QI. A national user-friendly, low burden approach to CR data collection incorporated into daily work-flow that is capable of benchmarking and informs QI is essential.

How Credibly Do CMIP6 Simulations Capture Historical Mean and Extreme Precipitation Changes?

AbstractFuture precipitation changes are typically estimated from climate model simulations, while the credibility of such projections needs to be assessed by their ability to capture observed precipitation changes. Here we evaluate how skillfully historical climate simulations contributing to the Coupled Model Intercomparison Project Phase 6 (CMIP6) capture observed changes in mean and extreme precipitation. We find that CMIP6 historical simulations skillfully represent observed precipitation changes over large parts of Europe, Asia, northeastern North America, parts of South America and western Australia, whereas a lack of skill is apparent in western North America and parts of Africa. In particular in regions with moderate skill the availability of very large ensembles can be beneficial to improve the simulation accuracy. CMIP6 simulations are regionally skillful where they capture observed (positive or negative) trends, whereas a lack of skill is found in regions characterized by negative observed precipitation trends where CMIP6 simulates increases.

Asia Pacific Sporolithon (Corallinophycidae, Rhodophyta) species revised based on DNA sequencing of type specimens and including S. crypticum sp. nov., S. immotum sp. nov. and S. nodosum sp. nov.

ABSTRACTTo address the taxonomic uncertainty of Sporolithon species named in the early to mid-20th century, targeted PCR sequencing was performed on eight historical type specimens and on recently collected specimens. Six type specimens amplified for the rbcL gene and were Sanger sequenced yielding sequences ranging in length from 118 to 280 base pairs (bp). One, S. australasicum, failed to amplify and another, S. howei, was amplified for the psbA gene yielding a sequence 544 bp in length. The 118 bp long rbcL sequence of the lectotype of S. crassiramosum showed that it is a later, heterotypic synonym of S. molle. The rbcL sequences of type specimens of S. episoredion, S. schmidtii, S. sibogae and S. timorense ranged from 118 to 228 bp, and each is a distinct species. The 544 bp long psbA sequence of S. howei is also unique. The 280 bp long rbcL sequence of the lectotype of S. durum did not match any sequence with that name in any public repository, including the previously published complete plastome and mitogenome sequences. However, it was identical in sequence to a specimen in GenBank from the southern coast of Western Australia as well as several other sequences generated from field-collected specimens from the states of South Australia and Western Australia. The rhodolith specimens from New Zealand previously called S. durum are S. nodosum sp. nov. The species is endemic to New Zealand. The epilithic specimens from New Zealand previously called S. durum are S. immotum sp. nov., which is also found along the southeastern coast of Australia. Sporolithon crypticum sp. nov. is described from the southern coast of Western Australia. RAxML and Bayesian phylogenetic analyses of Sporolithon psbA and rbcL sequences are congruent between the two plastid encoded genes. DNA sequencing of type specimens of species of corallines is demonstrated to be the only reliable method to correctly apply names.

Randomised controlled trials of behavioural nudges delivered through text messages to increase influenza and COVID-19 vaccines among pregnant women (the EPIC study): study protocol

BackgroundInfluenza and COVID-19 infections during pregnancy may have serious adverse consequences for women as well as their infants. However, uptake of influenza and COVID-19 vaccines during pregnancy remains suboptimal. This study aims to assess the effectiveness of a multi-component nudge intervention to improve influenza and COVID-19 vaccine uptake among pregnant women.MethodsPregnant women who receive antenatal care at five tertiary hospitals in South Australia, Western Australia and Victoria will be recruited to two separate randomised controlled trials (RCTs). Women will be eligible for the COVID-19 RCT is they have received two or less doses of a COVID-19 vaccine. Women will be eligible for the influenza RCT if they have not received the 2023 seasonal influenza vaccine. Vaccination status at all stages of the trial will be confirmed by the Australian Immunisation Register (AIR). Participants will be randomised (1:1) to standard care or intervention group (n = 1038 for each RCT). The nudge intervention in each RCT will comprise three SMS text message reminders with links to short educational videos from obstetricians, pregnant women and midwives and vaccine safety information. The primary outcome is at least one dose of a COVID-19 or influenza vaccine during pregnancy, as applicable. Logistic regression will compare the proportion vaccinated between groups. The effect of treatment will be described using odds ratio with a 95% CI.DiscussionBehavioural nudges that facilitate individual choices within a complex context have been successfully used in other disciplines to stir preferred behaviour towards better health choices. If our text-based nudges prove to be successful in improving influenza and COVID-19 vaccine uptake among pregnant women, they can easily be implemented at a national level.Trial registrationClinicalTrials.gov Identifier NCT05613751. Registered on November 14, 2022.

Two new Western Australian species related to Hibbertia priceana (Dilleniaceae)

Hibbertia priceana is a rare species found in two localised areas in southern south-western Western Australia, near Ongerup and in the vicinity of Wickepin and Harrismith. Two new species that are morphologically similar to H. priceana but are disjunct and distinct from it are described in this paper. Hibbertia hapalophylla is a new, potentially geographically restricted and rare species first collected during botanical surveys for a mining project near Mount Holland in the Western Australian Goldfields. It was initially collected from, and only known from, disturbed areas on an active mine site, but larger populations were subsequently discovered on a nearby sandplain, from where is was likely introduced to the mine site on sand transported for construction purposes. The second new species, Hibbertia remanens, appears to be restricted to small areas of remnant vegetation near Cunderdin and Kellerberrin in the Western Australian wheatbelt. The three species differ mainly in their leaf shapes in section, with H. priceana having flat leaves with the abaxial lamina fully exposed, H. remanens having recurved leaf margins with the abaxial shallowly grooved either side of the midrib, and H. hapalophylla having strongly revolute margins with the abaxial lamina surface concealed within lacunae formed between the margins and midrib.

Indigenous Women and Their Nutrition During Pregnancy (the Mums and Bubs Deadly Diets Project): Protocol for a Co-designed mHealth Resource Development Study.

Nutrition in pregnancy is pivotal to optimizing infant growth and maternal well-being. The factors affecting Indigenous people's food and nutrition intake are complex with a history of colonization impacting the disproportionate effect of social determinants to this day. Literature regarding the dietary intake or dietary priorities of Indigenous women in Australia is scarce, with supportive, culturally appropriate resources developed for and with this group rare. Research suggests mobile health (mHealth) tools are effective in supporting health knowledge of Indigenous people and positive health behavior changes when designed and developed with the expertise of Indigenous communities. This study seeks to build the body of knowledge related to nutrition needs and priorities for Indigenous women in Australia during pregnancy. Further, this project team and its participants will co-design an mHealth digital tool to support these nutrition needs. The Mums and Bubs Deadly Diets study recruits Indigenous women and health care professionals who support Indigenous women during pregnancy into 2 phases. Phase 1 (predesign) uses a mixed methods convergent design using a biographical questionnaire and social or focus groups to inform phase 2 (generative). Phase 2 will use a participatory action research process during co-design workshops to iteratively develop the digital tool; the exact actions within a workshop will evolve according to the participant group decisions. To date, this project has undertaken phase 1 focus groups at all Queensland sites, with New South Wales and Western Australia to begin in early to mid-2023. We have recruited 12 participants from Galangoor Duwalami, 18 participants from Carbal in Toowoomba, and 18 participants from Carbal in Warwick. We are expecting similar numbers of recruits in Western Australia and New South Wales. Participants have been both community members and health care professionals. This study is an iterative and adaptive research program that endeavors to develop real-world, impactful resources to support the nutrition needs and priorities of pregnant Indigenous women in Australia. This comprehensive project requires a combination of methods and methodologies to ensure Indigenous voices are heard at each stage and in all aspects of research output. The development of an mHealth resource for this cohort will provide a necessary bridge where there is often a gap in access to nutrition resources for women in pregnancy in Indigenous communities. DERR1-10.2196/45983.

The beautiful noctuid moth Chasmina pulchra (Doubleday) (Lepidoptera: Noctuidae) is a highly dispersive species, now established on urban kurrajongs Brachychiton populneus in southern Australia

ABSTRACT We report the establishment of the beautiful noctuid moth Chasmina pulchra in southern Australia following repeated self-introduction. First recorded in 1955 as a vagrant in suburban Adelaide, specimens have been collected or observed at an increasing rate since then. We have recently located a breeding population on the Adelaide Plains where the larvae feed on the introduced kurrajong tree Brachychiton populneus (Malvaceae), although other Brachychiton species are likely to be exploited elsewhere in its range. Native to the drier parts of eastern Australia, this drought-tolerant tree has been widely grown as a specimen or amenity tree in suburban Adelaide since the mid-nineteenth century. Chasmina pulchra appears to have an annual life cycle in South Australia, with adult moths active in the summer, December – March. Larvae cause characteristic scalloping on the leaf margins of the food plant. We present evidence that this tropical to subtropical native species has expanded its range into the southern half of the continent in the last few decades. We also report the first specimen collected in northern Tasmania, associated with a weather system that could have facilitated its movement across Bass Strait. Southward incursions are associated with both wetter and drier than average monthly rainfall totals just prior to the event. Together with recent records from southwestern Australia well beyond the natural range of a putative food plant, we conclude that C. pulchra is a highly dispersive species, behaviour consistent with at least one other species of Chasmina in the Old World.

Fire reduces eucalypt forest flowering phenology at the landscape-scale

Plant phenology describes the timing of reproductive events including flowering and fruiting, which for many species are affected by fire disturbance. Understanding phenological responses to fire provides insights into how forest demographics and resources may shift alongside increasing fire frequency and intensity driven by climate change. However, isolating the direct effects of fire on a species' phenology and excluding potential confounders (e.g. climate, soil) has been difficult due to the logistical challenges of monitoring species-specific phenological events across myriad fire and environmental conditions. Here, we use CubeSat-derived crown-scale flowering data to estimate the effects of fire history (time since fire and fire severity over a 15-year time span) on flowering of the eucalypt Corymbia calophylla across a Mediterranean-climate forest (814km2) in southwest Australia. We found that fire reduced the proportion of flowering trees at the landscape-scale, and flowering recovered at a rate of 0.15 % (±0.11% SE) per year. Further, this negative effect was significant due to high crown scorch fires (>20% canopy scorch), yet there was no significant effect from understory burns. Estimates were obtained using a quasi-experimental design which identifies the effect of time since fire and severity on flowering by comparing proportional flowering within target fire perimeters (treatment) and adjacent past fire perimeters (control). Given the majority of fires studied were managed fuel reduction burns, we applied the estimates to hypothetical fire regimes to compare flowering outcomes under more or less frequent prescribed burning. This research demonstrates the landscape-scale effects of burning on a tree species' reproduction, which could broadly impact forest resiliency and biodiversity.