South Of Spain Research Articles

Hierarchical distance and employees' commitment as conditioning of team-based work in the Spanish public sector.

Team-based work (TBW) plays a crucial role in the success and quality of public services. In the context of the Spanish public sector, our study evaluates if a low level of hierarchical distance (HD) in public organizations condition the public employees' commitment (EC) as well as TBW, assessed in terms of participation and consensus among team members. Simultaneously, we evaluated to what extent EC help to enhance TBW. Employing a sample of 213 government organizations from the south of Spain through a model of structural equations, we were able to answer the above questions. Our results revealed that team-based work functions more successfully within public organizations where there exists little verticality in their structures, norms, values and rules - in simplest terms, where there is minimal organizational HD. Such results also suggest that although regulations exist in the Spanish public sector, should public organizations attempt to be less vertical and more horizontal, they would perhaps have public servants that were more committed to their organizations. As a result, servants view the organization as their own and remain loyal. Finally, from an academic perspective, this study could be one of few to research and evaluate the hierarchical role and employees' commitment to the functioning of public employees based on participation and consensus in their work teams.

Multivariate analysis of the operational parameters and environmental factors of an industrial solar pond

The stability of an industrial Salinity Gradient Solar Pond (SGSP) has been studied by applying classical Principal Component Analysis (PCA) on three datasets with a different number of variables and time evolution. Temperature, density and heat extraction have been measured in the upper convective (UCZ), non-convective (NCZ) and low convective (LCZ) zones in a 500 m2 solar pond, located near Granada, South of Spain. Local environmental weather conditions have been also considered in the data analysis. Two operational seasons of the solar pond were considered in order to establish: (1) PCA exploratory models for 2014–2015 operational period and (2) to validate the obtained results during the 2015–2016 operational period. PCA results showed that three factors explained the variance in the monitored stability gradients. The first factor was related to the major effect of the seasonal temperature variation on the entire stability gradient. The second factor, related to the diurnal temperature variation, solar irradiance and wind variables, showed a strong impact on the solar pond temperature and salinity gradients and could affect strongly the UCZ and its border with the NCZ. The third factor affected the stability of salinity and temperature gradients at the LCZ and its border with the NCZ. The latter was related to the increase in temperature and salinity at the bottom of the solar pond, which suggests special attention during the initial formation and settlement of the salinity gradient and the subsequent heat extraction activities. This paper shows PCA modelling as a powerful tool for solar pond operation process surveillance and control.

Location of Biorefineries Based on Olive-Derived Biomass in Andalusia, Spain

A biorefinery integrated process based on lignocellulosic feedstock is especially interesting in rural areas with a high density of agricultural and agro-industrial wastes, which is the case for olive crop areas and their associated industries. In the region of Andalusia, in the south of Spain, the provinces of Jaén, Córdoba and Seville accumulate more than 70% of the olive wastes generated in Spain. Therefore, the valorisation of these wastes is a matter of interest from both an environmental and a social point of view. The olive biorefinery involves a multi-product process from different raw materials: olive leaves, exhausted olive pomace, olive stones and olive tree pruning residues. Biorefinery processes associated with these wastes would allow their valorisation to produce bioenergy and high value-added renewable products. In this work, using geographic information system tools, the biomass from olive crop fields, mills and olive pomace-extracting industries, where these wastes are generated, was determined and quantified in the study area. In addition, the vulnerability of the territory was evaluated through an environmental and territorial analysis that allowed for the determination of the reception capacity of the study area. Then, information layers corresponding to the availability of the four biomass wastes, and layers corresponding to the environmental fragility of the study area were overlapped and they resulted in an overall map. This made it possible to identify the best areas for the implementation of the biorefineries based on olive-derived biomass. Finally, as an example, three zones were selected for this purpose. These locations corresponded to low fragility areas with a high availability of biomass (more than 300,000 tons/year) in a 30 km radius, which would ensure the biomass supply.

Analysis of the Applicability of the Parabolic Trough Solar Thermal Power Plants in the Locations with a Temperate Climate

Currently, intensive work is underway in Poland to increase the share of renewable energy sources in the overall energy balance. Therefore, this paper presents the possibilities of using concentrated solar power in zones with a temperate climate. A simplified model based on the energy balance in the solar collectors considering the main operating parameters of the typical solar power plant was developed. It should be noted here that the model does not take into account issues related to heat accumulation and electricity generation in a Solar Thermal Power Station. The simulation of forced convection inside the solar collector absorber was additionally included in the calculations to improve its accuracy. The model was verified using actual heat measurements at the outlet of the parabolic collector installation at a Solar Thermal Power Station located in the south of Spain. The heat generated by a similar solar collector system in a selected region with a temperate climate, the city of Bialystok (north-eastern Poland, geographic coordinates: 53°08′07″ N 23°08′44″ E) was determined by the developed simplified model for different months of the year. Based on the results of the analysis, it was found that the energy obtained from the same area of concentrated solar collectors located near Bialystok is eight times lower compared to the location in Cordoba depending on the variant of the power plant operation.

Shared use of mineral supplement in extensive farming and its potential for infection transmission at the wildlife-livestock interface

Recently, the survival of Mycobacterium bovis on livestock mineral blocks has been confirmed, but little is known about its implication in the transmission of animal tuberculosis (TB) under field conditions. The objective of this study was to describe the shared use of mineral supplements in four extensive beef cattle farms from a high TB prevalence area in South Central Spain, to identify the main factors explaining their use, and characterize its potential role for the transmission of Mycobacterium tuberculosis Complex (MTC). This is relevant to design control measures at the wildlife-livestock interface. Animal activity was monitored by camera-trapping at 12 mineral supplementation points during spring and fall. Additionally, swabs were periodically taken from the mineral substrates and analyzed by PCR searching for MTC DNA. Cattle, pig, goat, sheep, wild boar, and red deer were all recorded licking on mineral supplementation points. Livestock species were the main users and presented a diurnal use pattern. Wild ungulates presented a nocturnal-crepuscular use pattern, with scarce overlapping with livestock. Wild boar presence was positively related to cattle presence at mineral supplementation points, whereas red deer presence was higher in supplemental points closer to forested areas and in farms without hunting pressure. We recorded 266 indirect wildlife-livestock interactions (i.e., two consecutive visits that occurred within 78 h), all of them derived from 21 unique wildlife visits. All the analyzed swabs resulted negative to MTC DNA. Comparing to other environmental sources of MTC in our study area, mainly water ponds, this research evidenced that mineral blocks are less attractive to wildlife. However, the potential for interspecific transmission of MTC or other pathogens cannot be discarded. The risk for interaction at mineral supplementation points and further transmission can be prevented by implementing specific measures in the context of integral biosecurity plans at the wildlife-livestock interface, which are proposed.

Stabilization Methodology in Foundation Soils by ERT-3D Application in Estepona, South Spain

The paper proposes a novel methodology for the stabilization of shallow foundations, with a simplified model combined with 3D electrical resistivity tomography (ERT-3D and consolidation injections. To determine its usefulness, the method has been applied in a case located in Estepona (southern Spain). The chosen tomography model is the dipole–dipole configuration, with an optimized distance between electrodes of 0.80 m for a better visualization of the foundation subsoil; with this parameterization, a total of 72 electrodes were installed in the analyzed case. In this work, the depth of the anomaly in the building’s supporting subsoil was detected ranging from 2.00 m to 3.90 m deep. The study also delineates areas of high resistivity variations (50–1000 Ω m) in the middle and eastern end of the field. These data have been validated and corroborated with a field campaign. The results of the ERT-3D monitoring are presented, once the investment data has been processed with the RES3DINV software, from the beginning to the end of the stabilization intervention. The novelty occurs with the interaction between the tomography and the foundation consolidation injections, until the final stabilization. This is a very useful methodology in case of emergency consolidation, where there is a need to minimize damage to the building. Thus, people using this combined system will be able to practically solve the initial anomalies of the subsoil that caused the damages, in a non-invasive way, considerably lowering the value of the resistivities.

Selecting Suitable MODFLOW Packages to Model Pond–Groundwater Relations Using a Regional Model

In large-scale regional models, used for the management of underground resources, it is quite common to find that relationships between the regional aquifer and small wetlands are not included. These models do not consider this connection because of the small amount of water involved, but they should consider the potential for significant ecological impacts if the groundwater resources in the ecosystems associated with these wetlands are mismanaged. The main objective of this work is to investigate the possibilities offered by MODFLOW LGR-V2 to represent (at small scale) the Santa Olalla pond, located in the Doñana Natural Park (South of Spain), and its relationship with the Almonte-Marismas regional aquifer. As a secondary objective, we propose to investigate the advantages and disadvantages that DRAIN, RIVER and LAKE MODFLOW packages offer within the MODFLOW LGR-V2 discretizations. The drain boundary condition with a coarse discretization implemented through ModelMuse allows the most adequate performance of the groundwater levels in the environment of the pond. However, when using lake boundary condition, the use of the MODFLOW LGR-V2 version is particularly useful. The present work also gives some guidelines to employ these packages with the MODFLOW graphical user’s interface, ModelMuse 4.2.

An Approach to the Operation Modes and Strategies for Integrated Hybrid Parabolic Trough and Photovoltaic Solar Systems

Concentrated solar power (CSP) and photovoltaic (PV) solar systems can be hybridized, creating synergies: on one hand procuring dispatchability by storing thermal energy, and on the other hand generating electricity at a highly competitive prize. In this paper, we present an approach to the operation strategies and modes for integrated hybrid CSP + PV systems. We focus on parabolic trough (PT) solar plants, especially those operating in the south of Spain. Our study consists in the definition of suitable states for each of the subsystems that constitute a hybrid solar plant. We then propose modes from the combination of suitable states and establish the conditions for the transition between modes depending on the operation strategy. We propose two operation strategies: demand coverage and base load production. The results of this paper can be used in decision making for hybrid solar system simulation programs.

Application of thermal storage in over-night refrigeration of an institutional building

The performance of different thermal energy storing systems to provide over-night air-conditioning for a limited space in an institutional building in south Spain are compared with conventional air-conditioning (both for refrigeration and heating). In summer, the storage tanks are charged with a solar-assisted absorption chiller during day-time, and used to cover the over-night demand, whereas in winter a heat exchanger is used during the day to charge the tanks. Thermal energy has been stored in two chilled water tanks (with total capacity of 5000 L) or in two tanks (total volume 4000 L) containing two phase change materials (PCM): a PCM with melting point of 10°C for refrigeration, and a PCM with melting temperature of 46°C for heating. Our results show that the storing in PCM is more convenient, providing air-conditioning service for approximately 4 h 30 min in refrigeration, with a significant reduction of the electricity consumption with respect to the conventional system. The chilled water tanks can cover the demand for 4 h (scaled down to 3 h 12 min if the capacity is 4000 L), with even lower electric consumption. In winter, on the other hand, the tanks can cover fully the demand of overnight heating. Our results prove the viability of thermal storing, in particular using PCM, to extend the application solar cooling and heating to night-time, which is economically feasible, in a fully operational building.

Mobility and Bioavailability of Metal(loid)s in a Fluvial System Affected by the Mining and Industrial Processing of Pb

The abandoned mining district of Linares (South Spain) is marked with waste from the mining and the processing of metal ores that pose an environmental hazard to watercourses. A combined analysis of waste, sediments and water was carried out to analyse the impact of a smelter on Baños Creek. The composition of the facility waste was determined using X-ray diffractometry and scanning electron microscopy. The total contents of the metal(loid)s in the waters and sediments of the watercourse were analysed, and sequential metal(loid) extraction of solid samples was carried out. The facility wastes consisted mainly of secondary minerals, such as natropharmacosiderite and spertiniite, as well as rare metal salts, such as mopungite and NaPb2(CO3)2(OH). The leachates generated by these wastes were highly alkaline, with a pH of 10 and a total dissolved solids concentration of approximately 9 g L−1. This Na-bicarbonate-type water had an As concentration above 200 mg L−1 and elevated levels of Pb, Sb and Zn (5029 µg L−1, 841 µg L−1 and 525 µg L−1, respectively). This highly contaminated lixiviate had a significant effect on the chemical quality of the waters and the bioavailability of metal(loid)s in the creek sediments, especially in the headwaters. In this zone, the As, Pb, Sb and Zn concentrations in the most mobile fraction of the sediments reached 1035 mg kg−1, 261 mg kg−1, 45 mg kg−1 and 30 mg kg−1, respectively. By comparison, smelter slag and mining waste have a much lower impact on the waters and the mobile fraction of the sediments, while significantly increasing the total concentration of these potentially toxic elements in creek sediments.

New records of phytoseiid mites (Acari: Mesostigmata) from Madeira Island

Madeira is the largest of the four islands constituting Madeira Archipelago in the North Atlantic Ocean. It is located at 400 km from the Northern Canary Islands, at 500 km from Morocco and between 900 and 1000 km from South Portugal and Spain. So far, nineteen species of the mite family Phytoseiidae had been reported from this island. We report in this paper the results of a survey conducted in May 2019 in Madeira Island, in which 15 species have been found, six being new for the Island fauna.

Wastewater Discharge through a Stream into a Mediterranean Ramsar Wetland: Evaluation and Proposal of a Nature-Based Treatment System

Impacts on wetlands are becoming more pressing every day. Among them, habitat loss, overexploitation of aquifers and changes in land use are considered the most important. However, the impacts linked to wastewater discharges are increasing worldwide. In this context, this study analyses the impacts of input of wastewater to a Mediterranean Ramsar temporary wetland (Fuente de Piedra, south of Spain). To this end, systematic sampling was carried out in the Charcón stream which receives water from a wastewater treatment plant (WWTP) and discharges it into the wetland. The results showed a slight decrease in the nutrient concentrations, particularly for nitrogen compounds. Heterotrophic and fecal bacteria concentration, as well as phytoplankton and zooplankton abundance and biomass, all significantly decreased from the treatment plant to the wetland. When comparing the effect of this discharge with other similar occurring to the same wetland, it was evident that the Charcón stream was responsible for a greater impact. At this point, it is relevant to note that the main difference among both treated wastewater discharges lies in the different water retention time once the wastewater was released from the WWTP. In fact, we recommend an increase in the water retention time by building seminatural ponds, together with the use of biofilters, which will notably contribute to improve the processes of assimilation of nutrients and to decrease the impact generated in the wetland by this spill.

Moral Disengagement Strategies in Online and Offline Bullying

Bullying and cyberbullying perpetration can involve cognitive processes of moral disengagement; however, there is no clear evidence about which strategies have the greatest influence on this type of behaviour. The aim of this paper was to examine which dimensions of moral disengagement were associated to bullying (off/online) and to explore the effect of gender and age. A total 1,274 students (48.6% girls, aged 11 to 17 years old) from the south of Spain were surveyed (M = 13.63, SD = 1.31). Multivariate multiple regression analyses showed that age and all moral disengagement mechanisms were associated with both offline and online bullying. Univariate regressions revealed that cognitive restructuring was the most strongly associated with both. The interaction between age and cognitive restructuring was only related to offline bullying. Simple slope analyses showed different effects for younger and older classmates at high levels of cognitive restructuring. Specific moral disengagement strategies have special significance for adolescent bullying and cyberbullying perpetration, with cognitive restructuring in particular promoting bullying perpetration in younger students. The results are discussed in relation to practical implications to prevent bullying and cyberbullying.

Experimental and analytical assessment of the influence of masonry façade infills on seismic behavior of RC frame buildings

On May 11, 2011 at 6:47p.m., an M5.1 earthquake took place in Lorca at a depth of 4 km, in the Region of Murcia, south of Spain, which resulted in nine fatalities and 324 injuries and major built environment damage. Approximately 80% of the residential buildings were affected with various degrees of damage, 5% with severe structural damage and 13% with moderate structural damage, in addition to the damage of infrastructure and public buildings. Reconnaissance and expert analyses revealed that one of the main causes of damage during the Lorca earthquake was the interaction between the non-structural elements and the seismic-resisting framing of the buildings. Studies on this interaction, that started in the 1950 s, led to the development of a wide range of macro-models that simulate the stiffness of the masonry infills using one or more equivalent diagonal strut(s). Following an extensive literature review, 12 mathematical expressions have been compiled to estimate the horizontal stiffness of the equivalent strut that simulates the behavior of masonry infills that make up a typical façade of many modern buildings. Two full-scale tests under horizontal cyclic displacement reversals have been carried out on two 5x3m concrete frames: one frame with masonry infill simulating a regular façade; and the other is a bare control frame. The comparison of both tests enabled quantifying the stiffness contribution of the façade infill and identifying the simplified expression that best fits the horizontal stiffness. Using this expression, a realistic seismic design of a prototype building in Lorca, Spain, is conducted under the typical assumption of most modern building codes to consider masonry infills as non-structural elements, and consequently the designed building was seismically assessed considering the influence of the infills. Results of the assessment are in accordance with the real damage patterns observed during the Lorca earthquake reconnaissance.

Alternative Food Networks: Perceptions in Short Food Supply Chains in Spain

Alternative spaces for the consumption of non-conventional foods as short commercialization chains have been increased worldwide, as well as the interest in understanding the dynamics developed in those initiatives from a social approach, has increased. This work aimed to analyze the Organizers, Producers and Consumers’ perceptions that participate in short food supply chains in ecological markets in the south of Spain. The Conventions Theory framework was used by applying a quantitative and qualitative methodology. A total of 159 questionnaires were applied (three to Organizers, 15 to Producers and 177 to Consumers). The questionnaire considered items related to social conventions for seven worlds (Domestic, Civic, Market, Industrial, Opinion and Inspired) and sociodemographic information. Kruskal-Wallis and Mann-Whitney tests were used to analyze the information. Results show that coincidences and divergences are observed in the importance given to the worlds; the Civic, Inspired and Opinion worlds are perceived similarly by the three types of actors and are related to the interest in how these activities benefit the environment. Differences are attributed to the role played by each type of actor and to socioeconomic aspects. Results can materialize into different strategies to improve these initiatives and reach more consumers.

Soil remediation approach and bacterial community structure in a long-term contaminated soil by a mining spill (Aznalcóllar, Spain)

The Aznalcóllar accident, which occurred in 1998, spilled 36 × 105 m3 of pyritic sludge and 9 × 105 m3 of acidic water around an area of 43 km2 in the south of Spain. This spill is considered one of the most important metal-mining associated accidents worldwide. In this study, two soil remediation techniques were evaluated: the addition of marble sludge (liming treatment, LS) and the mixing of recovered soils (RC) with contaminated soils (CT) (biopile treatment, BS). Both LS and BS significantly reduce the solubility of Cu, Zn, As, and Pb mainly due to the increase in pH and organic matter content, respectively. Soil basal respiration rate and the seed germination and root elongation bioassay with Lactuca sativa were used to evaluate the toxicity of the potential pollution in the sampled soils. Both bioassays showed that the CT soils exhibited the highest toxicity with a significant reduction in the toxicity of the amended soils (LS and BS). The abundance and structure of microbial communities in the soils were determined by qPCR and Illumina 16S rRNA sequencing, respectively. The absolute abundances of total bacterial and archaeal populations, ammonium oxidising bacteria, and denitrifiers in the CT soils were statistically lower than these found in the other three soils. Similarly, the structure of the bacterial community was highly different in the CT soils. Our results underline the persistence of the detrimental effect of pollutants in CT soils compared to the recuperated (RC) and amended soils (LS and BS). We also highlight the uses of liming or biopile as remediation techniques as satisfactory tools to reduce the impact of heavy metals in the contaminated Aznalcóllar soils.

Mining Wastes of an Albite Deposit as Raw Materials for Vitrified Mullite Ceramics

In this work, an examination of mining wastes of an albite deposit in south Spain was carried out using X-ray Fluorescence (XRF), X-ray diffraction (XRD), particle size analysis, thermo-dilatometry and Differential Thermal Analysis (DTA) and Thermogravimetric (TG) analysis, followed by the determination of the main ceramic properties. The albite content in two selected samples was high (65–40 wt. %), accompanied by quartz (25–40 wt. %) and other minor minerals identified by XRD, mainly kaolinite, in agreement with the high content of silica and alumina determined by XRF. The content of Na2O was in the range 5.44–3.09 wt. %, being associated with albite. The iron content was very low (<0.75 wt. %). The kaolinite content in the waste was estimated from ~8 to 32 wt. %. The particle size analysis indicated values of 11–31 wt. % of particles <63 µm. The ceramic properties of fired samples (1000–1350 °C) showed progressive shrinkage by the thermal effect, with water absorption and open porosity almost at zero at 1200–1250 °C. At 1200 °C, the bulk density reached a maximum value of 2.38 g/cm3. An abrupt change in the phase evolution by XRD was found from 1150 to 1200 °C, with the disappearance of albite by melting in accordance with the predictions of the phase diagram SiO2-Al2O3-Na2O and the system albite-quartz. These fired materials contained as main crystalline phases quartz and mullite. Quartz was present in the raw samples and mullite was formed by decomposition of kaolinite. The observation of mullite forming needle-shape crystals was revealed by Scanning Electron Microscopy (SEM). The formation of fully densified and vitrified mullite materials by firing treatments was demonstrated.

The Geological Heritage of Salobreña (South Spain): example of a touristic area

Salobreña consist of a touristic area with a typical Andalusian white town built on a hill and crowned with a Castle from the medieval era of Moors occupation. The urban area is bordered by fields of tropical crops and fruit trees that, together with its location by the sea, confer on Salobreña a beautiful postcard. These features, as a whole, are an attraction for visitors. Although the authorities are very interested in developing the beach tourism and displaying and protecting the historical center of the town, the interest in geological heritage is negligible. So, this paper tries to expose and evaluate the sites of geologic interest recognizable in the area, so that not only tourists and visitors, but also students, are offered more attractions for their visits. 30 sites are proposed as suitable to be considered as geological heritage, organized in three accessible walking itineraries named respectively: Urban, Panoramic and Littoral georoutes. The selected sites display a great variety of geological typologies as structural geology, stratigraphy, sedimentology, geomaterials, petrology, mineralogy, geomorphology and hydrogeology-hydraulics. Avoiding subjectivity, the sites and georoutes have been classified and ranked with a numerical methodology based on recent literature. The Scientific Value (SV), Potential Educational Value (PEV), Potential Touristic Value (PTV) have been quantified by using several criteria in each case. This allows visitors and teachers to select the most suitable route according to their goals. The Degradation Risk (DR) has also been evaluated, giving key geo-conservation actions. This plan can be presented to political and technical managers from Salobreña Town. Finally, some actions are proposed for better conservation of the sites but also to contribute to education and promote cultural tourism. These actions would also favor educational activities for students of all levels in earth science subjects, but also increase economic activity and sustainable development in the area by incorporating cultural visitors.

Biological and molecular characterization of tomato spotted wilt virus (TSWV) infecting Chrysanthemum in India

Tomato spotted wilt virus (TSWV) infecting chrysanthemum (Dendranthema grandiflora L.) has been identified as a major constraint for chrysanthemum cut-flower industries in Southern India. The disease symptoms include veinal necrosis, necrotic spots on leaves, deformed flowers, and stem necrosis. The presence of TSWV in chrysanthemum plants collected from farms at Kotagiri, Nedugula, and Yercaud in Tamil Nadu State, India, was confirmed by the triple antibody sandwich-enzyme-linked immunosorbent assay (TAS-ELISA). The pathogenicity of TSWV was established for three isolates (TN1KO, TN2NE, and TN3YE) by mechanical inoculation of sap into chrysanthemum cuttings. Host range experiments in different host plants indicated that the isolates expressed symptoms typical of TSWV in all hosts assayed. Furthermore, Nicotiana tabacum L. had maximum virus titre among the tested host plants, and therefore was identified as the best indicator and the propagative host. The nucleocapsid (N) gene of the three studied isolates shared 99.2–100% identity at the amino acid level among themselves and 96.5–99.6% identity with reference TSWV isolates. The non-structural movement protein (NSm) gene of studied isolates shared 99.0% identity among themselves and 96.0%–100% identity at the amino acid level with reference TSWV isolates. The phylogenetic relationship analyzed based on the amino acid sequence identity of the N gene revealed that the studied isolate clustered as a single group with all the TSWV isolates taken for comparison. However, analysis based on the NSm gene indicated that the Indian isolates grouped with isolates from South Korea, the USA, Italy, Spain, and Australia.

Melanosis Coli in Pigs Coincides With High Sulfate Content in Drinking Water

Melanosis coli is a well-described condition in humans, characterized by the accumulation of lipofuscin-laden macrophages in the lamina propria of the colon, giving it a dark tone. An increased apoptosis rate of colonic epithelial cells appears to be the underlying pathogenesis. In pigs, oxidative damage has been proposed as one of the causes for melanosis coli. In this article, we report a series of cases of melanosis coli in pigs affecting several finishing units in the south of Spain. Large intestines had dark green to brown pigmentation of the mucosa. Histological, histochemical, and ultrastructural studies confirmed a high number of lipofuscin-laden macrophages in the lamina propria of the rectum and colon, which additionally stained positive for the apoptosis marker cleaved caspase-3. Of note, all affected finishing units utilized water supply with a high content of sulfates, which may be one of the causes for melanosis coli development in pigs.