South China Sea Sediments Research Articles

Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome

Lipolytic enzymes, including esterases and lipases, represent a group of hydrolases that catalyze the cleavage and formation of ester bonds. A novel esterase gene, scsEst01, was cloned from a South China Sea sediment metagenome. The scsEst01 gene consisted of 921 bp encoding 307 amino acid residues. The predicted amino acid sequence shared less than 90% identity with other lipolytic enzymes in the NCBI nonredundant protein database. ScsEst01 was successfully co-expressed in Escherichia coli BL21 (DE3) with chaperones (dnaK-dnaJ-grpE) to prevent the formation of inclusion bodies. The recombinant protein was purified on an immobilized metal ion affinity column containing chelating Sepharose charged with Ni2+. The enzyme was characterized using p -nitrophenol butyrate as a substrate. ScsEst01 had the highest lipolytic activity at 35°C and pH 8.0, indicative of a meso-thermophilic alkaline esterase. ScsEst01 was thermostable at 20°C. The lipolytic activity of scsEst01 was strongly increased by Fe2+, Mn2+ and 1% Tween 80 or Tween 20.

Further Analysis of Anammox Bacterial Community Structures Along an Anthropogenic Nitrogen-Input Gradient from the Riparian Sediments of the Pearl River Delta to the Deep-Ocean Sediments of the South China Sea

The community structures of anammox bacteria in sediments along an anthropogenic inorganic nitrogen input gradient were further delineated with the newly available information incorporated. Anammox bacterial 16S rRNA gene-amplified sequences retrieved from riparian sediments of the Pearl River, Mai Po coastal wetland, and the South China Sea (SCS) sediments were compiled, compared and analyzed. Results indicated that the community structures of anammox bacteria varied from the upstream of the Pearl River to deep-ocean sediment of the SCS along the anthropogenic input grandient. Mai Po wetland had the most diverse anammox bacteria, followed by the shallow SCS, deep SCS and the Pearl River. Genera of the anammox bacteria Kuenenia and Brocadia showed higher proportion in the riparian sediments of the Pearl River, while those of Kuenenia and Scalindua dominated the Mai Po coastal wetland. The Scalindua subclusters showed apparent segregation in coastal wetland (S. zhenghei-III and S. wagneri), shallow SCS (S. zhenghei-I and S3) and deep SCS (S. zhenghei-I, S2 and S. arabica). Pearson correlation analysis indicated nitrogen species [NH4+ and ∑(NO2−+NO3− )] negatively correlated with the diversity indices of anammox bacteria. Canonical correspondence analysis (CCA) showed that salinity, inorganic nitrogen [NH4+, ∑(NO2−+NO3−)], and ratio of NH4+/∑(NO2− +NO3−) significantly affected the bacterial community compositions. Results collectively support that the community composition of anammox bacteria can serve as a bio-indicator to the anthropogenic terrestrial N input or pollution.

Existence of Novel Phylotypes of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in Surface and Subsurface Sediments of the South China Sea

Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered new microbial process performed by the Candidatus Methylomirabilis oxyfera with an unusual intra-aerobic pathway, but there is no report about n-damo bacteria in marine environments. M. oxyfera-like sequences were successfully retrieved for the first time from both surface and subsurface ocean sediments of the South China Sea (SCS) using both 16S rRNA and pmoA genes as biomarkers and PCR amplification in this study. The majority of M. oxyfera-like 16S rRNA gene-based PCR amplified sequences from the SCS sediments formed a new group distinctively different from those detected in freshwater habitats and the information is consistent phylogenetically with those obtained from the pmoA gene. This study showed the existence of n-damo in ocean sediments and suggests that marine sediments harbor n-damo phylotypes different from those in the freshwater. This finding here expands our understanding on the distribution of n-damo bacteria to marine ecosystem and implies their potential contribution to the marine C and N cycling.

Occurrence and diversity of nitrite-dependent anaerobic methane oxidation bacteria in the sediments of the South China Sea revealed by amplification of both 16S rRNA and pmoA genes.

Nitrite-dependent anaerobic methane oxidation (n-damo) process is unique in linking the microbial carbon and nitrogen cycles, but the presence of n-damo bacteria in marine ecosystem and the associated environmental factors are still poorly understood. In the present study, detection of n-damo bacteria using 16S rRNA and pmoA gene-based PCR primers was successfully employed to reveal their diversity and distribution in the surface and subsurface sediments of the South China Sea (SCS). The widespread occurrence of n-damo bacteria in both the surface and subsurface sediments with high diversity has been confirmed in this study. The pmoA gene-amplified sequences clustered within three newly erected subclusters, namely SCS-1, SCS-2, and SCS-3, suggesting the unique niche specificity of n-damo bacteria in the marine ecosystem. Results indicated the presence of n-damo bacteria in the west Pacific Ocean with a wide distribution from the continental shelf (E201S) to the deep abyss (E407S and E407B). Community structures of n-damo bacteria in SCS are clearly different from those of nonmarine ones known. It is also found that NO x (-) and NH4 (+) affected the community structures and distribution of n-damo bacteria in the SCS sediments differently. Salinity is another important factor identified, shaping the n-damo communities in marine environments. The community based on pmoA gene-amplified sequences, and community richness and diversity based on 16S rRNA gene-amplified sequences correlated with temperature.

A marine sediment component interpretation based on diffuse reflectance spectrophotometry from northern South China Sea sediment core MD972148

The sediment components of high resolution marine sediment cores are extremely important for revealing past climatic and environmental changes. However, analysis of deep sea sediments presents a number of exceptional challenges, especially for the northern South China Sea (SCS) which receives substantial amounts of suspended sediments from numerous rivers, including both the world's largest river in South China and a small mountain river from southwestern Taiwan. This study applies rapid and low cost color reflectance analysis in combination with multivariate analysis techniques to examine the overall trends of major mineral component variations in sediment core MD972148 from the northern SCS. The first-order derivative (central difference) reflectance data (from 400 to 700 nm) yields five major factors, which accounted for 89.7% of the variance for the input matrix. Collocating with varimax-rotated principal component analysis (VPCA) through SPSS software and comparing with the first-order derivative spectra of known minerals from a laboratory at Kent State University and the USGS library, the three leading factors represent kaolinite, illite + goethite, and hematite. Although the last two factors have no significant correspondences with known minerals, we found these two factors have similar variation patterns with the abundances of the foraminifer species Globigerinoides sacculifer and Globigerina bulloides in the downcore. Our research shows that diffuse reflectance spectrophotometry (DRS) is a useful method for rapidly examining the sediment components. To verify the mechanisms and connections between some sediment components and their color reflectances will require future research.

The Combined Effect of Tibetan Plateau Uplift and Glacial‐Interglacial Cycles on the Quaternary Evolution of the East Asian Monsoon: Evidence from South China Sea Sediments

Abstract:The siliciclastic sediments of the uppermost section of 185 mcd (meters composite depth) from ODP Site 1146 on the northern continental slope of the South China Sea (SCS) were partitioned according to their sources using end‐member modeling on grain‐size data. The goal was to evaluate the evolution of the East Asian monsoon over the past 2 million years. The siliciclastic sediments were described as hybrids of four end‐members, EM1, EM2, EM3, and EM4, with modal grain sizes of 8–22 μm, 2–8 μm, 31–125 μm, and 4–11 μm, respectively. EM1 and EM3 are interpreted as eolian dust and EM2 and EM4 as fluvial mud. The ratio of eolian dust to fluvial mud ((EM1+EM3)/(EM2+EM4)) is regarded as an indicator of the East Asian monsoon. The variation in this ratio not only shows periodical oscillations consistent with oxygen isotope stages, but also exhibits a phased increasing trend corresponding with the phased uplifts of the Tibetan Plateau, indicating that the evolution of the East Asian Monsoon was controlled not only by glacial‐interglacial cycles, but also by the phased uplifts of the Tibetan Plateau during the Quaternary.

Isotopic Composition and Distribution of Plutonium in Northern South China Sea Sediments Revealed Continuous Release and Transport of Pu from the Marshall Islands

The (239+240)Pu activities and (240)Pu/(239)Pu atom ratios in sediments of the northern South China Sea and its adjacent Pearl River Estuary were determined to examine the spatial and temporal variations of Pu inputs. We clarified that Pu in the study area is sourced from a combination of global fallout and close-in fallout from the Pacific Proving Grounds in the Marshall Islands where above-ground nuclear weapons testing was carried out during the period of 1952-1958. The latter source dominated the Pu input in the 1950s, as evidenced by elevated (240)Pu/(239)Pu atom ratios (>0.30) in a dated sediment core. Even after the 1950s, the Pacific Proving Grounds was still a dominant Pu source due to continuous transport of remobilized Pu from the Marshall Islands, about 4500 km away, along the North Equatorial Current followed by the transport of the Kuroshio current and its extension into the South China Sea through the Luzon Strait. Using a simple two end-member mixing model, we have quantified the contributions of Pu from the Pacific Proving Grounds to the northern South China Sea shelf and the Pearl River Estuary are 68% ± 1% and 30% ± 5%, respectively. This study also confirmed that there were no clear signals of Pu from the Fukushima Daiichi Nuclear Power Plant accident impacting the South China Sea.

Global dissolution effects on planktonic foraminiferal Mg/Ca ratios controlled by the calcite-saturation state of bottom waters

Mg/Ca ratios of planktonic foraminiferal tests are important tools for reconstructing past ocean temperatures at different levels of the upper water column. Yet numerous studies suggest a significant influence of calcite dissolution on Mg/Ca ratios lowering their initial signal recorded within a planktonic foraminiferal habitat. To determine the effect of dissolution, this study presents Mg/Ca ratios of eight planktonic foraminiferal species from the South China Sea sediment surface. Continuously decreasing with increasing water depth, the Mg/Ca ratios also decrease with calcite-saturation states close to and below saturation (bottom water Delta[CO32-] 40 mu mol kg(-1)). This preservation pattern compares well with examples of Mg/Ca dissolution from the tropical Atlantic Ocean and is independent of the foraminiferal species. Merging a global data set by separate normalization of 79 Mg/Ca data sets from the Pacific, Atlantic, and Indian Oceans, which removes thermal differences between the ocean regions and foraminiferal species, enabled us to quantify a global decrease in planktonic foraminiferal Mg/Ca ratios of 0.054 +/- 0.019 mu mol mol(-1) per mol kg(-1) below a critical threshold for dissolution of 21.3 +/- 6.6 mu mol kg(-1). The absolute decline in Mg/Ca ratios, which is similar for all species, affects temperature estimates from (sub-)thermocline species more strongly than those from shallow dwellers. The water depth of this critical threshold in the global oceans shoals from >3.5 km in the North Atlantic to <0.5 km in the North Pacific based on calculations of the global calcite-saturation state from 6321 hydrographic stations. Above this critical threshold Mg/Ca ratios are well preserved, and paleotemperature estimates are broadly unaffected by dissolution.

Acoustic response of gas hydrate formation in sediments from South China Sea

Gas hydrate has been recognized as a potential energy resource in South China Sea (SCS). Understanding the acoustic response of gas hydrate formation in the SCS sediments is essential for regional gas hydrate investigation and quantification. The sediments were obtained from gravity core sampling at E 115°12.52363′ N 19°48.40299′. Gas hydrate was formed within a “gas + water-saturated SCS sediments” system. Combination of a new bender element technique and coated time domain reflectometry (TDR) was carried out to study the acoustic response of hydrate occurrence in SCS sediments. The results show the acoustic signal becomes weak when hydrate saturation (Sh) is lower than 14%. The acoustic velocities (Vp, Vs) of the sediments increase with Sh during hydrate formation, and Vs increases relatively faster when Sh is higher than 14%. These results indicate that tiny hydrate particles may firstly float in the pore fluid, which causes a significant acoustic attenuation, but has little influence on shear modulus. As time lapses and Sh approaches 14%, numerous particles coalesce together and contact with sediment particles. As a result, Vs has a sharp increase when hydrate saturation exceeds 14%. Several velocity models were validated with the experimental data, which suggests a combination of the BGTL (Biot–Gassmann Theory modified by Lee) model and the Weighted Equation is suitable to estimate Sh in SCS.

Cytotoxic and Antibacterial Marfuraquinocins from the Deep South China Sea-Derived Streptomyces niveus SCSIO 3406

Four new sesquiterpenoid naphthoquinones, marfuraquinocins A-D (1-4), and two new geranylated phenazines, phenaziterpenes A (5) and B (6), were isolated from the fermentation broth of Streptomyces niveus SCSIO 3406, which originated from a South China Sea sediment sample obtained from a depth of 3536 m. The structures of 1-6 were elucidated on the basis of extensive MS and one-dimensional and two-dimensional NMR spectroscopic analyses. In a panel of cytotoxicity and antibacterial assays, 1 and 3 were found to inhibit a NCI-H460 cancer cell line with IC50 values of 3.7 and 4.4 μM, respectively. Compounds 1, 3, and 4 exhibited antibacterial activities against Staphylococcus aureus ATCC 29213 with equivalent MIC values of 8.0 μg/mL; compounds 3 and 4 each showed antibacterial activity against methicillin-resistant Staphylococcus epidermidis (MRSE) shhs-E1 with MIC values of 8.0 μg/mL.

Correction: Bacterial Community Composition of South China Sea Sediments through Pyrosequencing-Based Analysis of 16S rRNA Genes

Correction: Bacterial Community Composition of South China Sea Sediments through Pyrosequencing-Based Analysis of 16S rRNA Genes

Metagenomic Profiles of Antibiotic Resistance Genes (ARGs) between Human Impacted Estuary and Deep Ocean Sediments

Knowledge of the origins and dissemination of antibiotic resistance genes (ARGs) is essential for understanding modern resistomes in the environment. The mechanisms of the dissemination of ARGs can be revealed through comparative studies on the metagenomic profiling of ARGs between relatively pristine and human-impacted environments. The deep ocean bed of the South China Sea (SCS) is considered to be largely devoid of anthropogenic impacts, while the Pearl River Estuary (PRE) in south China has been highly impacted by intensive human activities. Commonly used antibiotics (sulfamethazine, norfloxacin, ofloxacin, tetracycline, and erythromycin) have been detected through chemical analysis in the PRE sediments, but not in the SCS sediments. In the relatively pristine SCS sediments, the most prevalent and abundant ARGs are those related to resistance to macrolides and polypeptides, with efflux pumps as the predominant mechanism. In the contaminated PRE sediments, the typical ARG profiles suggest a prevailing resistance to antibiotics commonly used in human health and animal farming (including sulfonamides, fluoroquinolones, and aminoglycosides), and higher diversity in both genotype and resistance mechanism than those in the SCS. In particular, antibiotic inactivation significantly contributed to the resistance to aminoglycosides, β-lactams, and macrolides observed in the PRE sediments. There was a significant correlation in the levels of abundance of ARGs and those of mobile genetic elements (including integrons and plasmids), which serve as carriers in the dissemination of ARGs in the aquatic environment. The metagenomic results from the current study support the view that ARGs naturally originate in pristine environments, while human activities accelerate the dissemination of ARGs so that microbes would be able to tolerate selective environmental stress in response to anthropogenic impacts.

Bacterial Community Composition of South China Sea Sediments through Pyrosequencing-Based Analysis of 16S rRNA Genes

BackgroundSubseafloor sediments accumulate large amounts of organic and inorganic materials that contain a highly diverse microbial ecosystem. The aim of this study was to survey the bacterial community of subseafloor sediments from the South China Sea.Methodology/Principal FindingsPyrosequencing of over 265,000 amplicons of the V3 hypervariable region of the 16S ribosomal RNA gene was performed on 16 sediment samples collected from multiple locations in the northern region of the South China Sea from depths ranging from 35 to 4000 m. A total of 9,726 operational taxonomic units (OTUs; between 695 and 2819 unique OTUs per sample) at 97% sequence similarity level were generated. In total, 40 bacterial phyla including 22 formally described phyla and 18 candidate phyla, with Proteobacteria, Firmicutes, Planctomycetes, Actinobacteria and Chloroflexi being most diverse, were identified. The most abundant phylotype, accounting for 42.6% of all sequences, belonged to Gammaproteobacteria, which possessed absolute predominance in the samples analyzed. Among the 18 candidate phyla, 12 were found for the first time in the South China Sea.ConclusionsThis study provided a novel insight into the composition of bacterial communities of the South China Sea subseafloor. Furthermore, abundances and community similarity analysis showed that the compositions of the bacterial communities are very similar at phylum level at different depths from 35-4000 m.

Anthropogenic Fingerprint in Beibu Gulf (South China Sea) Sediments

Xia, Z.; Waniek, J.J., and Leipe, T., 2013. Anthropogenic fingerprint in Beibu Gulf (South China Sea) sediments. In: Harff, J., Leipe, T., Waniek, J.J., and Zhou, D. (eds.), Depositional Environments and Multiple Forcing Factors at the South China Sea's Northern Shelf, Journal of Coastal Research, Special Issue, No. 66, pp. 72–90. Coconut Creek (Florida), ISSN 0749-0208.Surface sediments and five cores were chosen to determine grain size, geochemical elements, heavy metals concentrations and age using 14C dating to study sedimentary environment and anthropogenic impact in the Beibu Gulf (South China Sea). The grain size analysis shows differences in the depositional environment amongst the subareas of the gulf. In the north of the gulf, the depositional environment is strong and unstable with complicated hydrodynamics because of the combined influence of rivers, tide, littoral current and the monsoon. In the central part, the depositional environment is weaker and stable, whereas in the south of the gulf (at the entrance), the deposition is higher and influenced mainly by tide. The deposition rate is around 0.3 mm/yr based on 14C dating. The geochemical element analysis indicates different sediment sources in different subareas of the gulf and possible influence of a biogenic source. The terrigenous elements (Al, Fe, Li, Ti, K and Zr) have high positive correlation coefficients, and originate from the northern coast and Hainan Island. The trace elements are mostly enriched within the fine sediments. The enrichment factors (EF) and cultural enrichment factors (CEF) based on aluminum and titanium show that total organic carbon (TOC), As and Hg have high concentrations in the surficial sediments in the north (9.92 mg/kg and 34 μg/kg) and the south (15.5 mg/kg and 22 μg/kg) of the gulf, and are enriched below the surface. Despite the regional differences in heavy metal concentrations in surface sediments, no anthropogenic impact was observed in the center of the gulf according to the results of EF and CEF using the core data. Measured concentrations of the anthropogenic elements were below the evaluation criterion values of the National Standards of GB18668-2002, P. R. China indicating low anthropogenic impact in the entire gulf.

03219A, a new Δ8,9-pregnene isolated from Streptomyces sp. SCSIO 03219 obtained from a South China Sea sediment

03219A (1), a new pregnene steroid possessing a rare Δ(8,9)-double bond in the skeleton, together with the known naphthoquinone antibiotic (+)-cryptosporin (2) have been isolated from the fermentation broth of Streptomyces sp. SCSIO 03219, which was isolated from a marine sediment collected in the South China Sea. The structure of 03219A was elucidated using a combination of NMR, MS and X-ray crystallographic methods.

More refined diversity of anammox bacteria recovered and distribution in different ecosystems

A newly reported 16S rRNA gene-based PCR primer set was successfully applied to detect anammox bacteria from four ecosystem samples, including sediments from marine, reservoir, mangrove wetland, and wastewater treatment plant sludge. This primer set showed ability to amplify a much wider coverage of all reported anammox bacterial genera. Based on the phylogenetic analyses of 16S rRNA gene of anammox bacteria, two new clusters were obtained, one closely related to Candidatus Scalindua, and the other in a previously reported novel genus related to Candidatus Brocadia. In the Scalindua cluster, four new subclusters were also found in this study, mainly by sequences of the South China Sea sediments, presenting a higher diversity of Candidatus Scalindua in marine environment. Community structure analyses indicated that samples were grouped together based on ecosystems, showing a niche-specific distribution. Phylogenetic analyses of anammox bacteria in samples from the South China Sea also indicated distinguished community structure along the depth. Pearson correlation analysis showed that the amount of anammox bacteria in the detected samples was positively correlated with the nitrate concentration. According to Canonical Correspondence Analysis, pH, temperature, nitrite, and nitrate concentration strongly affected the diversity and distribution of anammox bacteria in South China Sea sediments. Results collectively indicated a promising application of this new primer set and higher anammox bacteria diversity in the marine environment.

Tracing the Nd isotope evolution of North Pacific Intermediate and Deep Waters through the last deglaciation from South China Sea sediments

The intermediate and deep waters of the Pacific Ocean play a crucial role for regulating global climate changes on the millennium timescale. However, due to poor preservation of carbonate sediments in the deep Pacific, little attention has been received to better understand deep ocean circulation and its relationship with abrupt climate events. Here we present the first authigenic Nd isotope (εNd) record extracted from Fe–Mn oxyhydroxides of the South China Sea (SCS) sediments (SO17940-2, 1727m water depth) in order to investigate changes of intermediate and deep-water circulations in the Pacific Ocean since the Last Glacial Maximum (LGM).In general, the seawater εNd record extracted from the SCS sediments paralleled the existing Nd isotopes of fish teeth/debris from the eastern North Pacific core during the last deglaciation, demonstrating that our authigenic εNd can faithfully record the seawater εNd evolution in the deep Pacific Ocean. At the onset of the deglacial period, our data exhibit a clear negative shift in εNd towards a gradually increased component of Antarctica Intermediate Water (AAIW) and reached a maximal influx during the mid Heinrich Stadial 1 (HS1, ∼16kyr BP). Between the HS1 and Younger Dryas, deep-water circulation gradually shifted back to the modern condition during the Bølling-Allerød warm interval. A pronounced negative εNd excursion had occurred during the Pre-Boreal (PB), suggesting a rapid reorganization of deep-water ventilation in the Pacific Ocean and was predominated by AAIW. This is most likely due to poor ventilation in the western North Pacific associated with an intensified summer monsoon during the PB-early Holocene warm period. After the PB period, the seawater εNd values were once again dominated by Pacific Deep Water at the study site.

Fluostatins I–K from the South China Sea-Derived Micromonospora rosaria SCSIO N160

The strain SCSIO N160 was isolated from a South China Sea sediment sample and was characterized as a Micromonospora rosaria species on the basis of its 16S rRNA gene sequence. Three new fluostatins, I-K (1-3), were isolated from the culture of M. rosaria SCSIO N160, together with six known compounds, fluostatins C-F (4-7), rabelomycin (8), and phenanthroviridone (9). The structure of fluostatin D (5) was confirmed by an X-ray crystallographic study. The absolute configuration of 1 and 3 was assigned by electronic circular dichroism calculations. Compounds 8 and 9 exhibited good antimicrobial activities against Staphylococcus aureus ATCC 29213 with MIC values of 1.0 and 0.25 μg/mL, respectively. Compound 9 also exhibited significant in vitro cytotoxic activities toward SF-268 (IC50 0.09 μM) and MCF-7 (IC50 0.17 μM).

Nd, Sr isotopes and elemental geochemistry of surface sediments from the South China Sea: Implications for Provenance Tracing

The mineralogy, major and trace elements, and neodymium and strontium isotopes of surface sediments in the South China Sea (SCS) are documented with the aim of investigating their applicability in provenance tracing. The results indicate that mineralogical compositions alone do not clearly identify the sources for the bulk sediments in the SCS. The Nd isotopic compositions of the SCS sediments show a clear zonal distribution. The most negative εNd values were obtained for sediments from offshore South China (−13.0 to −10.7), while those from offshore Indochina are slightly more positive (−10.7 to −9.4). The Nd isotopic compositions of the sediments from offshore Borneo are even higher, with εNd ranging from −8.8 to −7.0, and the sediments offshore from the southern Philippine Arc have the most positive εNd values, from −3.7 to +5.3. This zonal distribution in εNd is in good agreement with the Nd isotopic compositions of the sediments supplied by river systems that drain into the corresponding regions, indicating that Nd isotopic compositions are an adequate proxy for provenance tracing of SCS sediments. Sr isotopic compositions, in contrast, can only be used to identify the sediments from offshore South China and offshore from the southern Philippine Arc, as the 87Sr/86Sr ratios of sediments from other regions overlapped. Similar zonal distributions are also apparent in a La–Th–Sc discrimination diagram. Sediments from the west margin of the SCS, such as those from Beibuwan Bay, offshore from Hainan Island, offshore from Indochina, and from the Sunda Shelf plot in the same field, while those offshore from the northeastern SCS, offshore from Borneo, and offshore from the southern Philippine Arc plot in distinct fields. Thus, the La–Th–Sc discrimination diagram, coupled with Nd isotopes, can be used to trace the provenance of SCS sediments. Using this method, we re-assessed the provenance changes of sediments at Ocean Drilling Program (ODP) Site 1148 since the late Oligocene. The results indicate that sediments deposited after 23.8Ma (above 455 mcd: meters composite depth) were supplied mainly from the eastern South China Block, with a negligible contribution from the interior of the South China Block. Sediments deposited before 26Ma (beneath 477 mcd) were supplied mainly from the North Palawan Continental Terrane, which may retain the geochemical characteristics of the materials covered on the late Mesozoic granitoids along the coastal South China. For that the North Palawan Continental Terrane is presently located within the southern Philippine Arc but was located close to ODP Site 1148 in the late Oligocene. The weathering products of volcanic material associated with the extension of the SCS ocean crust also contributed to these sediments. The rapid change in sediment source at 26–23.8Ma probably resulted from a sudden cessation of sediment supply from the North Palawan Continental Terrane. We suggest that the North Palawan Continental Terrane drifted southwards along with the extension of the SCS ocean crust during that time, and when the basin was large enough, the supply of sediment from the south to ODP Site 1148 at the north slope may have ceased.

Carbon and oxygen isotope characteristics of foraminiferan from northern South China Sea sediments and their significance to late Quaternary hydrate decomposition

Carbon and oxygen isotope and dating analyses of foraminiferan in sediment cores collected from three different areas of the northern slope of the South China Sea were conducted, in order to examine the records of the gas hydrate decomposition events since the late Quaternary under the conditions of methane seepage. The results show that: 1) the δ 13C values of the benthic foraminiferan Uvigerina spp. (size range of 0.25–0.35 mm) are from −0.212% to −0.021% and the δ 18O values of the planktonic foraminiferan Globigerinoides ruber (size range of 0.25–0.35 mm) are from −0.311% to −0.060%; 2) three cores (ZD2, ZD3 and ZS5) from the bottom of a hole are aged for 11 814, 26 616 and 64 090 a corresponding to the early oxygen isotope stage (MIS) I, III and IV final period, respectively; 3) a negative-skewed layer of carbon isotope corresponds to that of MIS II (cold period), whose degree of negative bias is −0.2‰; and 4) the δ 13C compositions of foraminiferans are similar to those of the Blake Ridge and the Gulf of Mexico sediments of the late Quaternary. According to the analysis, the reasons for these results are that the studied area is a typical area of methane seep environment in the area during MIS II due to the global sea-level fall and sea pressure decrease. Gas hydrate is decomposed and released, and a large number of light carbon isotopes of methane are released into the ocean, dissolved to inorganic carbon (DIC) pool and recorded in the foraminiferan shells. A pyrite layer developed in the negative bias layers of the foraminiferans confirms that the δ 13C of foraminiferans is more affected by methane and less by the reduction of marine productivity and early diagenesis. The use of foraminiferan δ 13C could accurately determine late Quaternary hydrate release events and provide evidence for both reconstructing the geological history of methane release events and exploring natural gas hydrate.