Bio3 Liquid Organic Fertilizer is a liquid organic fertilizer made from fermented fruit waste with local activator JB3. This fertilizer was tested on carrot plants to determine the effect of giving several concentrations of POC Bio3 on carrot yields. The research was conducted in July-November 2022 in Tobu Village, South Central Timor District, East Nusa Tenggara Province, Indonesia. This study used a single factor randomized block design (RBD) consisting of 9 concentrations of liquid organic fertilizer (LOF) Bio3 (0, 5, 10, 15, 20, 25, 30, 35, 40 ml/L water) and 3 replications. The parameters studied were tuber diameter, tuber length, tuber wet weight per plot. The results obtained were that the application of Bio3 liquid organic fertilizer to carrot plants had a significant effect on tuber diameter (3.30 cm), tuber length (16.22 cm), tuber weight per plant (84.61 g) and tuber weight per plot (4,230 kg). Based on effectiveness, efficiency and results that are not significantly different, a concentration of 25 ml/L of liquid organic fertilizer Bio3 is the best concentration.