The article deals with cooperation between Russia and Tajikistan in the economic and humanitarian spheres during the post-Soviet thirty years. In the section on joint economic projects, the author focuses on cooperation in energy and high-tech industries, as well as Russian investments in the economy of Tajikistan. A special place in the article is given to the analysis of the formation of the Central Eurasian macro-region and the prospects for the participation of Russia and Tajikistan in that process. According to the author, Russian-Tajikistani cooperation in Central Eurasia can not only bring tangible economic benefits to Moscow and Dushanbe, but also effectively promote the integration of Central and South Asian states as a whole. In the author’s view it would be economically feasible to create an extensive network of the transit and communication development corridors, known as “East-West” and “NorthSouth”, on the vast and resource-rich territory of Central Eurasia, which would help solve the communication issue that is very acute for many Eurasian states. Humanitarian cooperation is widely understood by the author as cooperation in the field of science, education and culture, as well as in the field of ensuring security for the human, society and state. In such a context, he examines Russia’s involvement in the resolution of the inter-Tajik conflict in detail and also highlights cooperation between Russia and Tajikistan in the fight against international crime, especially drug trafficking and illegal migration, including within the framework of the Commonwealth of Independent States