ABSTRACT Agricultural industries contribute to water pollution, releasing agrochemicals, organic matter, nutrients and veterinary drugs. Our study aimed to evaluate water quality and ecotoxicological effects of a stream that receives agro-industrial effluents from dairy production at industrial scales and pluvial runoff of agricultural activities. The present study addresses a comprehensive analysis of physicochemical parameters, metals, pesticides, veterinary drugs and microbiological aspects at three sampling sites located upstream, near a dairy drainage discharge and downstream. Lethal chronic ecotoxicity bioassays and biomarkers on Rhinella arenarum larvae were evaluated. Poor water quality near the industrial discharge points was found, characterised by elevated ammonium, biological oxygen demand, chemical oxygen demand and high concentrations of metals that exceeded environmental guidelines. In all sites, microbial aspects, total coliforms and fecal coliforms were detected, along with detergents, pesticide residues and ivermectin in high concentrations, exhibiting diverse profiles. Chronic toxicity bioassays showed mortality on amphibian larvae. The antioxidant defence system and the cholinergic pathways were altered in exposed larvae. The study highlights the urgent need to improve effluent management and regulations in the region to protect freshwater and underground water sources, aquatic fauna and human health.
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