Source Of Rumen Fluid Research Articles

Influence of Rumen Fluid Source and Fermentation Time on In Vitro True Dry Matter Digestibility

The effect on true dry matter digestibility of Philippine feedstuffs was studied with different sources of rumen fluid inocula and varying fermentation time in an in vitro sysytem. In one experiment differences in true dry matter digestion in vitro were nil among three sources of rumen fluid inocula (buffalo, cattle-Philippines, cattle-Cornell University).For commonly fed tropical feedstuffs in the other experiment rumen fluid from the cattle at Cornell gave lower digestibilities. True dry matter digestibility in vitro increased for each 24h increase in fermentation periods of 48, 72, and 96h. Differences in digestibility between cattle and buffalo in metabolism studies (in vivo) cannot be explained by differences in rumen fluid.

Influence of rumen fluid source upon establishment and cultivation in vitro of the rumen protozoon Entodinium.

SYNOPSIS. Favorable conditions for establishing Entodinium cultures in vitro were provided by rendering the inoculum almost free of other genera of rumen ciliates, inoculating at a final concentration of 30,000 entodinia per ml, providing 0.2 mg rice starch per ml, but principally by including an appropriate rumen fluid in the media. Rumen fluids collected from animals with high concentrations of entodinia in vivo, in general, were superior in supporting entodinia in vitro than were those collected from animals having low concentrations of entodinia in vivo. Rumen fluids from lambs were improved by clarification (centrifuging at 25,000 ×g for 20 min), but clarification destroyed the ability of rumen fluids from pregnant ewes to support entodinia in vitro. One or more factors occurring in the supernatant portion of pooled, clarified lamb rumen fluid stimulated entodinia in vitro.

The influence of rumen fluid source upon viable bacteria counts and the cultivation of oligotrich and holotrich rumen protozoa

In the course of series of experiments in this laboratory, concerned with the cultivation of rumen protozoa “in vitro” (1, 2), it was indicated that the source of rumen fluid included in the media may have influenced the results obtained. For this reason, the experiments reported here were carried out to determine if such an effect existed, and if so, to obtain some indication of the mode of action of such a possible effect.