Source Of Fatty Acids Research Articles

Investigation of physicochemical properties, nutritional status, and volatile compounds profiling of four underutilized spices of Cameroon origin

In this study, four underutilized spices (fruit and ulb of Aframomum sulcatum, fruit of Xylopia africana, and bark of Hypodaphnis zenkeri) were analyzed for their physical properties, nutrients composition, and profile of volatile compounds using standard analytical methods. The protein, fat, sugar, and dietary fiber contents were analyzed. The spices were revealed to be a good source of essential amino acids and fatty acids including pentadecanoic acid. Several minerals (Mn, Mg, Ca, Fe, and K) of nutritional importance were found at various proportions. Electronic nose analysis revealed the presence of fifty-six different compounds, belonging to ten major classes (phenylpropenes, acids, alcohols, aldehydes, esters, ketones, terpenes, pyrazine, sulfur-containing compounds, and ether) and also some flavor-characterizing compounds responsible for a variety of sensory descriptors. Bioactive volatile compounds like terpinen-4-ol, dimethyl trisulphide, and P-cymene were found in these spices, which can be further applied in the development of functional foods and nutraceuticals.

A Comprehensive Study of Techniques to Optimize the Extraction of Lipids from the Autotrophic Strain of the Microalgae Chlorella vulgaris.

Microalgae represent a promising source of triglycerides and free fatty acids, synthesized in the form of lipids, for use in renewable fuels and chemicals. One challenge is the ability to efficiently recover the lipids from within the microalgae cell. Although various techniques have been studied individually, a comprehensive study of extraction techniques using consistent experimental and analytical methodology is missing. This study aims to provide this unifying comparison using the common microalgae strain Chlorella vulgaris. The factors that were surveyed and then optimized to achieve maximum extraction efficiency included the solvent type; mechanical pre-treatment using a ball mill at a variety of grinding speeds; microalgae-to-solvent ratio; extraction facilitated by microwave; extraction facilitated by sonication; extraction facilitated using increased temperature; and extraction facilitated by in situ transesterification to convert the lipids into esters prior to extraction. The optimum conditions determined during these studies were utilizing methanol as the solvent, with ball mill pretreatment at a grinding speed of 500 rpm, and a 1:9 microalgae to solvent ratio. When used in combination with microwave-assisted extraction at a temperature of 140 °C, approximately 24 wt% of the initial lipids were recovered. Recoveries of over 70 wt% were obtained without a microwave at extraction temperatures of over 200 °C.

Biochemical composition, oil profiling and elemental analysis of different cumin (Cuminum cyminum L) genotypes

Cumin (Cuminum cyminum L.) is an annual plant of the family Apiaceae and the genus Cuminum has a single species Cyminum, native from the east Mediterranean to east India. In India, Gujarat and Rajasthan are the major producing states. After black pepper, cumin is the second most popular spice in the world. The present research work was carried out to study the nutritional quality parameters, oil profiling and element composition of fifteen cumin genotypes. The results showed that the moisture content was found to vary from 6.22 to 8.15 %. The carbohydrate content was higher in Kushalpura-1 (46.14 %), while the crude protein was highest in Indawar followed by GC-2 and Merta-2. Total protein content was highest in Lampolai (18.24 %) and lowest in GC-2 (11.36 %). In Lamba Jatan, highest content of non-reducing sugars (8.35 %) and total soluble sugars (9.11 %) were observed. Reducing sugars was detected in the range of 0.72 – 1.53 %. Highest amount of total free amino acids and crude fiber were found in Gawardi and Kushalpura-1, respectively. The total oil (20.27 %) and volatile oil (3.99 %) content were highest in GC-4. The petroselinic acid and linoleic acid were observed as primary fatty acids in all tested genotypes. However, Lampolai, Merta-1, GC-2 and GC-4 were good sources of both fatty acids among all the genotypes. The elemental analysis showed that the GC-2 has a high overall amount of macronutrients, while micronutrients was highest in Piplon-5.

Research of the Impact of Full Fat Flax Meal from Oilseed Flax Seeds on the Rye Bread Quality

Bakery products can act as a convenient object for modification and development of qualitatively new products with programmable properties on its basis. A flaxseeds processing product – flaxseed flour can be one of the possible sources of dietary fiber and polyunsaturated fatty acids. The research aim is to study the flaxseed flour impact on the rye bread quality. The thesis presents the impact results of non-fat flaxseed flour obtained from the new seeds varieties of oilseed flax of VNIIMK on the organoleptic and physico-chemical quality indicators of rye bread. A man prepared the dough starter culture from the Livendo® LV4 starting culture. The best flavor was in bread containing 15% flaxseed flour of dark-colored flax seeds of the VNIIMK 620 FN variety. The decrease in porosity of bread crumb with dark flaxseed flour was 1; 3 and 5% in variants with 5; 10 and 15% flour, whereas there was a decrease of 6; 7 and 9% in variants with flour from light-colored seeds, respectively, compared with the control. The authors revealed that adding flour from light-colored seeds reduced porosity more significantly than in bread variants with the flour from dark-colored flax seeds added. The volume of bread weighing 100 g varied from 143 to 170 cm3 . Calculations of nutritional and energy value demonstrated that the carbohydrates amount in bread with flaxseed flour is lower compared to the control (without flaxseed flour). Considering the lack of protein, polyunsaturated fatty acids, dietary fiber in the Russians diet, the bread with non-fat flaxseed flour has prospects for the market introduction.

Seasonal variation in the metabolome expression of Jania rubens (Rhodophyta) reveals eicosapentaenoic acid as a potential anticancer metabolite

Seaweeds of the intertidal zone are subjected to diverse stresses due to environmental changes in radiation, salinity, water quality, herbivore communities, etc. Thus, marine seaweeds developed various unique compounds to deal with environmental fluctuations. Therefore, they are a good source of unique novel compounds. Here, we explored the seasonal metabolomic changes in Jania rubens and found notable changes between extracts of different seasons in the metabolomic profile and in their anticancer activity. The most bioactive extract was from samples collected during the Fall season, which demonstrated an LC50 of 178.39 (± 10.02 SD) µg/ml toward Non Small Cell Lung Cancer (NSCLC) followed by the Winter season extract. The Fall and Winter extracts also displayed more resemblance in their metabolic profile relative to Spring and Summer extracts. The Fall extract was fractionated and tested for cytotoxic activity toward an array of cancer cell lines. Eventually, using a bio-guided assay and multiple fractionation steps, we isolated and identified the essential fatty acid, eicosapentaenoic acid, as the active anticancer agent, showing an LC50 of 5.23 (± 0.07 SD) µg/ml toward NSCLC. Our results emphasize the potential use of J. rubens as a source of beneficial fatty acids and stress the importance of environmental effects on metabolic constitutes.

Evaluation of Fatty Acid and the Composition of Six Different Species of Freshwater Fish in the North of Algeria


 
 
 Background: Few studies have been published about the quality of freshwater fish in Algeria. This study determined the chemical composition and the fatty acid of six species of freshwater fish cultivated in the North region of Algeria (Nile tilapia, red tilapia, common carp, Algerian barb, crucian carp, and mirror carp) as well as the nutritional quality of the lipids in these freshwater fish species.
 Methods: One hundred and ten freshwater fish were randomly caught in the spring of 2021 from Achor Ali farm (Jijel), Beni-Haroun Dam (Mila), and EL-Agrem Dam (Jijel) from Algeria. Moisture, ash, protein, lipid, and fatty acids were measured according to standard laboratory procedures and protocols of previous studies. Statistical analysis was performed using ANOVA (XLSTAT 2014), and the pair wise comparison of the means was done by Tukeys test at the 5% significance level (p<0.05).
 Results: Regarding freshwater fish species, fatty acid profiles were discovered to have 38.94 to 57.75% Saturated Fatty Acids (SFAs), 29.35 to 46.63% Monounsaturated Fatty Acids (MUFAs), and 6.79 to 26.55% Polyunsaturated Fatty Acids (PUFAs). Common carp was a rich resource of Eicosapentaenoic Acid and Docosahexaenoic Acid (EPA+DHA) (5.38%); the highest concentration of ω-3 was recorded in crusian carp (10.63%); and Nile tilapia contained significant levels of ω-6 PUFA. Results demonstrated that the examined freshwater fish species appeared to have a good nutritional value and be a source of important fatty acids with positive effects on consumer health. On the other hand, results revealed low levels of PUFAs.
 Conclusion: The examined freshwater fish species appeared to have a good nutritional value, but, it is important to provide diets rich in fatty acids, in particular PUFAs, to these freshwater fish to improve the nutritional quality of their lipids.
 
 

Hemp seed (Cannabis sativa L.) cake as sustainable dietary additive in slow-growing broilers: effects on performance, meat quality, oxidative stability and gut health

Hemp seed cake (HSC) (Cannabis sativa L.) is a rich source of polyunsaturated fatty acids, high-quality proteins and essential amino acids. The aim of this study was to evaluate the effects of dietary inclusion of HSC on growth performance, meat quality traits, fatty acids profile and oxidative status, and intestinal morphology in slow-growing broilers. A total of 180 male slow-growing broilers were randomly assigned to one of three dietary treatments containing different levels of HSC: 0 (HSC0), 5 (HSC5) or 10% (HSC10). Birds were slaughtered at 49 days of age: breast and thigh muscles were analysed and duodenum mucosa histomorphological features were evaluated. Regardless the level of HSC inclusion, no differences among groups were found for performance and meat quality traits. The thigh and breast fatty acid profile were significantly improved in both HSC groups, with an increase of the long chain fatty acids of n-3 series and decrease of n-6/n-3 ratio. The HSC diets lowered the MDA concentration and lipid hydroperoxides in breast meat. Histomorphometrical analysis revealed a significant increase in villus height, surface area and villus/crypt ratio, with a decrease of crypt depth, suggesting that dietary supplementation with HSC may boost intestinal health status in poultry. In conclusion, dietary HSC did not affect performance, carcass traits and meat quality, while it positively influenced the lipid profile of meat, and improved the oxidative status and gut health, thus representing a valuable and sustainable alternative ingredient in broiler diet.

Embryo yield and quality in superovulated Simmental donor cows following oral supplementation with different sources of omega-3 and omega-6 fatty acids

Owing to contradictory results with respect to the yield and quality of embryos after supplementation with omega-3 fatty acids, this study sought to determine the effect of an oral supplement containing sources of omega-3 and omega-6 fatty acids on the ovarian response and embryo quality after superovulation. Cows were allocated to receive one of three oral supplement regimens. In the control (CO) group (n = 9), cows were fed a control supplement without fatty acids. The cows in the soybean oil (SO) (n = 8) and linseed oil (LO) (n = 8) groups received supplements containing SO or LO, respectively. Supplements were administered for 28 days, commencing 7 days before the onset of estrus synchronization until the day of embryo collection. The estrous cycle of all cows was synchronized by inserting a controlled internal drug release (CIDR) device (progesterone 1.38 g), followed by the administration of estradiol benzoate 2.5 mg. The CIDR device was withdrawn 7 days after insertion, followed by intramuscular administration of dinoprost 25 mg. Superovulation was induced via the administration of 152 mg of follicle-stimulating hormone (Folltropin-V) in tapered doses, starting 72 h before CIDR withdrawal. The embryos were collected 6 days after artificial insemination. The estrous response, onset of estrus after CIDR withdrawal, and number of ovulations were similar among the experimental groups (p > 0.05). The mean number of transferable embryos (grades 1 and 2) was higher in the LO group compared to the CO group (9.0 vs 3.2; p < 0.05). Although the number of transferable embryos (grades 1 and 2) was greater in the LO group than that in the SO group, the difference was not statistically significant (9.0 and 4.4; p > 0.05). In conclusion, oral supplementation with LO for 28 days increased the yield of transferable embryos, which could potentially improve the profitability of embryo transfer programs in cattle.

Characterization of monogalactosyldiacylglycerol synthases in Gracilariopsis lemaneiformis and their potential roles in the fading of the thallus.

Membrane lipids play essential roles in regulating physiological properties in higher plants and algae. Monogalactosyldiacylglycerol (MGDG) is a major thylakoid membrane lipid, and it is an important source of polyunsaturated fatty acids for cells, plays a key role in the biogenesis of plastids, and maintains the function of the photosynthetic machinery. Several studies have indicated that the knockdown of MGDG synthase results in membrane lipid remodeling, albino seedlings, and changes in photosynthetic performance. However, the effects of MGDG synthase (MGD) inhibitors on lipids in macroalgae have not yet been clarified. Here, we characterized the effects of MGD inhibitors (ortho-phenanthroline and N-ethylmaleimide) on the composition of the fatty acids observed in MGDG and digalactosyldiacylglycerol (DGDG) in Gracilariopsis lemaneiformis using electrospray ionization-mass spectrometry. The most abundant MGDG species contained 16:0/18:1 (sn-1/sn-2) fatty acids, and the most dominant DGDG species contained 20:5/16:0 (sn-1/sn-2) fatty acids. Measurements of photosynthetic pigments and photosynthetic parameters revealed that photosynthesis of G. lemaneiformis was impaired. Principal component analysis and Spearman's correlation analysis revealed interactions between specific MGDG structural composition patterns and key metabolites involved in photosynthesis, indicating that 20:4/16:0 (sn-1/sn-2) MGDG and 16:0/18:1 (sn-1/sn-2) MGDG affect the structure and function of phycobilisomes and thus the color of G. lemaneiformis. Three genes (GlMGD1, GlMGD2, and GlMGD3) were cloned and identified. The addition of N-ethylmaleimide to G. lemaneiformis did not affect the abundance of GlMGD mRNA, and the abundance of transcripts was significantly decreased by ortho-phenanthroline.

Long-matured cured meats from Poland and Europe compared – An overview

This review aimed to present different varieties of long-matured cured meats, such as cold cuts, sausages, and hams produced in Poland, and compare them with similar European products. Moreover, we briefly presented the impact of meat products on human health. Due to its significant production in Poland, most meat products are made of pork. Polish long-matured, cured meats are nutritious, provide essential amino acids and micronutrients, and are a balanced source of monosaturated fatty acids (MUFAs). We suggest choosing cured Polish meat products matured with probiotics to provide pro-health benefits. Although long-matured sausages are essential and valuable meat products in Poland, they should be consumed moderately as an element of a balanced, diverse diet.

Assessment of Fatty Acid Composition, Bioactive Compounds, and Mineral Composition in Hazelnut Genetic Resources: Implications for Nutritional Value and Breeding Programs

This study was carried out to determine the fatty acid composition, bioactive compounds, and mineral element content of standard hazelnut cultivars and accessions from the Eastern Black Sea region. A wide variation was determined in terms of the traits examined between hazelnut accessions and cultivars. Most of the accessions investigated had higher values of bioactive compounds, oleic acid, stearic acid, Na, and Ca than the standard cultivars. Among accessions investigated, S-1 had the highest total phenolics and antioxidants (557.3 mg/100 g and 0.53 mmol/100 g) while P-4 had the lowest (307.3 mg/100 g and 0.22 mmol/100 g). The highest oleic acid content was determined in P-2 (87.16%); the lowest was in H-1 (71.24%). Linoleic acid ranged from 4.35% (P-1) to 18.06% (H-1). P-2 (3349 mg/kg and 2464 mg/kg, respectively) had the highest K and P contents. The highest Mg content was found in S-1 (1787 mg/kg). The highest value of Ca and Na was determined in P-1 (2701 mg/kg and 412 mg/kg, respectively). Principal component analysis revealed that the traits studied could effectively explain the variability among hazelnut genetic sources. First, three components explained about 60% of total variation. PC1 was related to arachidonic acid and most of the mineral elements (P, K, Mg, Ca, Fe, Mn, Zn, Cu, B, Cd, Ni, and S), and explained 27.6% of the total variation. PC2 explained 18.3% of the total variation, and was mainly related to oleic, linoleic, and linolenic acid, as well as Ca. PC3 was related to total phenolics, total flavonoids, antioxidant activity, and palmitic acid, and explained 14.5% of total variation. The oleic acid had a strong negative correlation with linoleic acid (−0.99 ***) and linolenic acid (−0.95 ***). A strong positive correlation was determined between antioxidant activity and total flavonoids (r = 0.95 ***). K content showed a strong positive correlation with P (r = 0.92 ***) and Mg (r = 0.82 ***) contents. A strong positive correlation was also determined between P and Mg (r = 0.91***). These findings revealed that many of the accessions investigated were a good source of fatty acids and bioactive compounds. As a conclusion, most accessions with superior nutritional content can be evaluated as genetic material for the development of new cultivars in hazelnut breeding programs.

Quality of Glycine max (soybean) and Helianthus annuus (sunflower) oils stored in different environments and their suitability for consumption

Soybean and sunflower oils contribute to the diet in many countries, serving as a source of protein, lipids and fatty acids, and repair worn-out tissues and are a useful source of energy. Their excessive or inadequate consumption may provide increased risk of developing various diseases. Sunflower seed provides 20-40% of oil and soybeans provide oil with immense advantages due to their high content of essential fatty acids. This work aimed to evaluate the physicochemical quality of soybean and sunflower oils and their effects on health. 5 samples of oils: A, sealed sunflower oil exposed to ambient light; B, sealed sunflower oil without exposure to sunlight; C, sealed soybean oil exposed to ambient light; D, unsealed sunflower oil exposed to ambient light and E, sealed soybean oil without exposure to light were evaluated by determining the acidity index, through titration with NaOH 0. 1N, peroxide index, by titration with Na2S2O3 0.01N, moisture, by dissection at 105ºC, rancidity, by qualitative method and vitamin A by iCheck Chroma. The NM 425:2012 legislation was used to verify the suitability or otherwise of the samples. Moisture ranged from 0.1 to 0.92 w/w, acidity index from 0.17 to 0.34mgKOH, peroxide index from 4 to 15.2 meq.O2/Kg, vitamin A between 13.27 and 29.95mgRe/Kg and positive rancidity. The results showed that the acidity index and vitamin A provided adequacy and the other parameters showed inadequacy for consumption.

The type of lipid supplement has crucial implications for forage particle size in calf starter diets

BackgroundForage inclusion in starters of young dairy calves has become an acceptable strategy in the last decade. To compensate for the lower energy provided by forage, concurrent lipid supplementation can be proposed. However, ruminal microbial activity and forage digestibility may be decreased by lipid supplementation. We hypothesized that the composite effect of forage and lipid supplements may be dependent on forage particle size and the type of lipid supplement. Therefore, we evaluated the effect of long (LP; geometric mean, 4.97 mm) vs. short alfalfa hay particle sizes (SP; geometric mean, 1.26 mm) with either soybean oil (SBO) or palm fatty acids (PLF) as lipid source in a 2 × 2 factorial design with treatments SP-SBO, SP-PLF, LP-SBO, and LP-PLF. Treatments (n = 13 with 6 males and 7 females each) were offered to Holstein calves (3 days old) with equal amounts of lipid (25 g/kg DM) throughout the experimental period. The milk offering scheme (d 1 to 53) was equal for all groups. Data collection continued until 20 d post-weaning.ResultsInteraction between forage particle size and lipid supplement was significant for the following readouts: the highest and lowest starter intakes during the pre-weaning period occurred in LP-PLF and LP-SBO, respectively. This was associated with similarly contrasting changes in average daily gain (ADG) during the post-weaning period, body weight at the end of experiment, withers height, digestibility of organic matter and neutral detergent fiber, and blood serum concentrations of glucose, beta-hydroxybutyrate, and insulin during the pre-weaning period. During both pre- and post-weaning periods, the highest and lowest urinary excretion of allantoin and total purine derivatives, representing microbial protein synthesis, were observed in LP-PLF and LP-SBO, respectively, indicating that those diets were most and least favorable for rumen development. Irrespective of forage particle size, supplemental SBO vs. PLF increased serum malondialdehyde as an oxidative stress indicator across periods, increased blood urea nitrogen and feed efficiency in the pre-weaning period, and reduced hip height during the post-weaning period.ConclusionsIt can be concluded that feeding a rumen-inert, mostly saturated fatty acid source with alfalfa hay as long particle size is recommended with view on performance, whereas a combination soybean oil rich in unsaturated fatty acids should not be provided to milk-fed Holstein calves together with long particle forage. Feeding soybean oil and alfalfa hay as long particles is not advisable mainly due to lower starter consumption and impaired development of ruminal function. If dietary supplementation of soybean oil is applied, incorporation of forage as small particles should be preferred to support rumen development.

Effect of Germination on Fatty Acid Composition in Cereal Grains.

Sprouted grains are gaining popularity as functional food ingredients. This study aimed to evaluate the lipid and fatty acid composition of eight sprouted grains (millet, amaranth, quinoa, wheat, rye, barley, buckwheat, and oat). The method used was germination for up to 72 h at temperatures ranging from 19-23 °C. In general, the lipid content increased in the various grains sprouted, providing a rich source of polyunsaturated fatty acids. The % oil yield ranged from 1.17 ± 0.02% in sprouted rye to 5.71 ± 0.26% in sprouted amaranth. Germinated oat showed the greatest increase in fat content, 54.3%, compared to the control. Polyunsaturated fatty acids were more prevalent in whole grains (46.9-75.6%) than saturated fatty acids (10.1-25.9%) and increased with sprouting. The primary fatty acids detected in the grains, in order of abundance, were linoleic, oleic, palmitic, linolenic, and stearic acids. Millet sprouts contained the lowest total saturated fatty acids and the highest polyunsaturated fatty acids. Amaranth had the highest amount of saturated fatty acids, while buckwheat contained the lowest quantity of polyunsaturated fatty acids. The lowest omega-6/omega-3 ratio was 7 to 1 in sprouted rye and 8 to 1 in sprouted barley.

Maternal rat prenatal and neonatal treatment with pequi pulp reduces anxiety and lipid peroxidation in brain tissue of rat offspring at adolescence

The Pequi fruit (Caryocar Brasiliense cambess), typical of the Brazilian cerrado or savannah, is a source of essential fatty acids, carotenoids, and phenolic compounds. The aim of this study was to analyze the effects of consuming this fruit on anxiety behavior and lipid peroxidation in the brains of rats whose mothers were treated (by gavage) during pregnancy and lactation with Pequi fruit (pulp or nuts) at 2000 mg/kg of body weight. Anxiety parameters were assessed using the open field (OF), elevated plus maze (EPM), and light/dark box (LDB) tests. The brain was removed to measure malondialdehyde (MDA) levels. Data were analyzed using One-way Anova (p < 0.05). In the OF, the animals in the pulp group presented more time spent in the central area (20.37 ± 0.73 vs Control: 12.51 ± 0.39; Nuts: 8.28 ± 0.40) and increased locomotion (159.7 ± 6.10) compared to the other groups (Control: 127.3 ± 5.54; Nuts: 139.08 ± 6.57). In the EPM, the pulp group entered into the open arms (8.57 ± 0.36) and stayed more time in the central area (19.44 ± 1.17) compared to the Nuts group (7.14 ± 0.34; 13.00 ± 1.57). In the LDB the pulp group entered more (8.00 ± 0.42 vs Control: 7.16 ± 0.16 and Nuts: 7.42 ± 0.75) and stayed longer in the clear light side (92.18 ± 6.42) than all the other groups (Control: 71.44 ± 3.53; Nuts: 80.57 ± 6.50), respectively. Pulp group presented lower MDA in the brain (55.34 ± 3.04) compared to Control (72.06 ± 4.66) and Nuts (66.57 ± 2.45). We conclude that Pequi pulp consumption during pregnancy and lactation reduces lipid peroxidation in brain tissue and induces anxiolytic-like behavior in rat offspring. These effects were not observed in the Pequi nuts group.

Microbial production of docosahexaenoic acid (DHA): biosynthetic pathways, physical parameter optimization, and health benefits.

Omega-3 fatty acids, including docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and α-linolenic acid (ALA), are essential polyunsaturated fatty acids with diverse health benefits. The limited conversion of dietary DHA necessitates its consumption as food supplements. Omega-3 fatty acids possess anti-arrhythmic and anti-inflammatory capabilities, contributing to cardiovascular health. Additionally, DHA consumption is linked to improved vision, brain, and memory development. Furthermore, omega-3 fatty acids offer protection against various health conditions, such as celiac disease, Alzheimer's, hypertension, thrombosis, heart diseases, depression, diabetes, and certain cancers. Fish oil from pelagic cold-water fish remains the primary source of omega-3 fatty acids, but the global population burden creates a demand-supply gap. Thus, researchers have explored alternative sources, including microbial systems, for omega-3 production. Microbial sources, particularly oleaginous actinomycetes, microalgae like Nannochloropsis and among microbial systems, Thraustochytrids stand out as they can store up to 50% of their dry weight in lipids. The microbial production of omega-3 fatty acids is a potential solution to meet the global demand, as these microorganisms can utilize various carbon sources, including organic waste. The biosynthesis of omega-3 fatty acids involves both aerobic and anaerobic pathways, with bacterial polyketide and PKS-like PUFA synthase as essential enzymatic complexes. Optimization of physicochemical parameters, such as carbon and nitrogen sources, pH, temperature, and salinity, plays a crucial role in maximizing DHA production in microbial systems. Overall, microbial sources hold significant promise in meeting the global demand for omega-3 fatty acids, offering an efficient and sustainable solution for enhancing human health.

Optimization of ultra-sonicated homogenization conditions of fish oil emulsions to improve stability, efficiency and bioaccessibility of ω -3 microcapsules

This study aimed to improve the quality characteristics of fish oil microcapsules from monolayer and multilayer emulsions by optimizing the ultra-sonicated homogenization conditions to obtain a stable source of omega-3 fatty acids to add in food matrix. For that, a response surface methodology trial was firstly conducted to get optimum ultra-sonicated conditions that increase stability of emulsions and efficiency of microcapsules. Thus, optimum combinations of power of (141.01 and 141.21 kg m2/s3), time (15 and 5 min), pulse (80%) and emulsion volume (100 and 200 mL) were obtained for monolayered and multilayered emulsions, respectively. In comparison to homogenization by high-pressure, the use of ultrasounds improved most quality characteristics of emulsions (stability and drop size) and microcapsules (microencapsulation yield, total oil, microencapsulation efficiency, quantities and bioaccessibility of EPA and DHA, lipid oxidation, size, and distribution), especially in monolayered ones. It worth noting the increase in microencapsulation efficiency in multilayered and the improvement in emulsion stability and bioaccessibility of EPA and DHA in both types of microcapsules.

Minimizing tannery sludge in landfilling through a mixed microbial culture approach: Effect of oxidizing pretreatment, temperature and hydraulic retention time on process performances and chromium fate

Exploiting tannery sludge as potential source of short-chain fatty acids (SCFAs) and biogas offers a promising solution for a new management of this waste, which is currently landfilled due to chromium presence. This study assessed the optimal hydrogen peroxide (H2O2) dosage, temperature (T) and hydraulic retention time (HRT) through hydrolysis and acidification tests to maximize SCFAs production and to recover biogas from the solid-rich fermentation residue.A dosage of 0.4 g H2O2/g TS and mesophilic T (40 °C) were the most suitable conditions to promote sludge biodegradability and SCFAs production in batch tests. Afterwards, among the two investigated HRT (4 and 8 days) in the semi-continuous processes, longer HRT increased SCFAs concentration and acidification yield up to 16.0 g CODSCFAs/L and 0.32 g CODSCFA/g VS0, respectively. Chromium was initially released in the liquid phase, but completely removed in less than 5 HRTs, allowing the utilization of fermentation liquid without environmental-safety issues. Finally, the solid-rich residue showed its potential as biogas source, having a specific gas production (SGP) of 0.48 m3/kg VS. According to the mass balance assessment, more than 50% of sludge can be saved from landfill, with potential economic benefit close to 2,700,000 €/y compared to the current management practice.

Comparing effects of beetroot juice and Mediterranean diet on liver enzymes and sonographic appearance in patients with non-alcoholic fatty liver disease: a randomized control trials.

In both developed and developing countries, non-alcoholic fatty liver disease (NAFLD) has lately risen to the top of the list of chronic liver illnesses. Although there is no permanent cure, early management, diagnosis, and treatment might lessen its effects. The purpose of conducting the current study is to compare the effects of beetroot juice and the Mediterranean diet on the lipid profile, level of liver enzymes, and liver sonography in patients with NAFLD. In this randomized controlled trial, 180 people with a mean age of (45.19 ± 14.94) years participated. Participants ranged in age from 19 to 73. The mean weight before intervention was (82.46 ± 5.97) kg, while the mean weight after intervention was roughly (77.88 ± 6.26) kg. The trial lasted for 12 weeks. The participants were split into four groups: control, a Mediterranean diet with beet juice (BJ + MeD), Mediterranean diet alone (MeD), and beetroot juice (BJ). The Mediterranean diet included fruits, vegetables, fish, poultry, and other lean meats (without skin), sources of omega-3 fatty acids, nuts, and legumes. Beetroot juice had 250 mg of beetroot. Data analysis was done using SPSS software (version 26.0). p < 0.05 is the statistical significance level. Following the intervention, Serum Bilirubin, alkaline phosphatase (ALP), alanine transaminase (ALT), serum cholesterol (CHOL), triglyceride (TG), and low-density lipoprotein (LDL) levels were significantly decreased in the BJ + MeD, BJ, and MeD groups (p = 0.001). Also, high-density lipoprotein (HDL) significantly increased in the BJ + MeD, BJ, and MeD groups (p = 0.001), while decreasing in the Control group (p = 0.001). The research findings indicate a significant reduction in hepatic steatosis among the groups receiving beetroot juice (BJ) and beetroot juice combined with the Mediterranean diet (BJ + MeD). This suggests that beetroot juice holds potential as an effective treatment for non-alcoholic fatty liver disease (NAFLD) in adults. Furthermore, the combination of beetroot juice with the Mediterranean diet showed enhanced efficacy in addressing NAFLD.Clinical trial registration: ClinicalTrials.gov, identifier NCT05909631.

Co-production of lactate and volatile fatty acids through repeated-batch fermentation of fruit and vegetable waste: Effect of cycle time and replacement ratio

In this study, repeated-batch fermentation was used to convert fruit and vegetable waste to lactate and volatile fatty acids (VFAs), which are essential carbon sources for medium-chain fatty acids (MCFAs) production. The effect of cycle time and replacement ratio on acidification in long-term fermentation was investigated. The results showed that they had a significant impact on product yield, productivity, and type of products. Considering the yield, productivity, and lactate/VFAs ratio, a replacement ratio of 30% and a cycle time of 2 d may be more suitable for further production of MCFAs. Its productivity and lactate/VFAs ratio were 4.07 ± 0.24 g/(L·d) and 5 ± 0.6, respectively. The lactic acid bacteria, such as Enterococcus (63%) and Lactobacillus (33%), stabilized in the reactor, resulting in the generation of both lactate and VFAs by heterolactic fermentation. The present study demonstrated a new strategy with the potential to recover high-value products from organic waste streams.