Learning biochemistry can be a challenge for most university students. They represent a lot of difficulty in studying this module. This is evidenced by numerous failures and a lack of interest in the subject. For them, biochemistry is a dry subject requiring extensive memorization and complex language. They also find it boring. However, biochemistry is a key element in the training of biology students and future teachers of life and earth sciences. It is the basis for understanding the mechanisms of life. It helps students to understand the chemical processes that take place in living beings. The aim of our study is to identify students' difficulties in learning biochemistry, in order to deduce the main causes and factors behind the low proficiency in this subject. For this purpose, we communicated with the undergraduate students at our school using NGT and a questionnaire. Students attribute their difficulties mainly to the complexity of the subject and the large amount of information that must be assimilated in a limited time. In addition, the problem of the language of instruction as well as the lack of tutorials and practical work can represent additional factors hindering the learning process. The lack of basic knowledge and the classical teaching methods can also be considered sources of difficulty. At the end of our study, we were able to elucidate the main factors and obstacles that hinder the learning of biochemistry. The students have suggested some solutions including the adoption of new teaching methods promoting active learning, as well as by diversifying the pedagogical supports used in class and the integration of new communication and information technologies (ICT).
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