Source Of Nutrition Research Articles

Revolutionizing livestock sustainability: Pioneering breeding strategies for superior forage biomass and quality

Livestock primarily rely on forage crops as a source of feed and nutrition. The milk productivity of a cow or meat production in goat/sheep could directly be associated with the availability of a sufficient quantity of quality green fodders with essential nutrients in a balanced ratio. Feeding the cereal/grass: legume fodders in the required proportion will not only improve productivity but also the reproductive capacity of animals. However, many countries of the world experience a huge gap between demand and availability of green fodder. In this context, emphasis should be placed on developing efficient forage genotypes with increased biomass and quality as per the requirements of animals, duly considering their digestibility. Breeding approaches encompassing required classical approaches, including wide hybridization to exploit natural genetic variability, biotechnological tools such as transgenic technology, marker-assisted selection, genomic selection, and various omics techniques alongside high-throughput phenotyping using multispectral cameras, would help to sustain livestock productivity by meeting out the present and future fodder requirements coupled with enhanced nutrients

A reproducible extended ex-vivo normothermic machine liver perfusion protocol utilising improved nutrition and targeted vascular flows

BackgroundNormothermic machine perfusion of donor livers has become standard practice in the field of transplantation, allowing the assessment of organs and safe extension of preservation times. Alongside its clinical uses, there has been expanding interest in extended normothermic machine perfusion (eNMP) of livers as a potential vehicle for medical research. Reproducible extended normothermic machine perfusion has remained elusive due to its increased complexity and monitoring requirements. We set out to develop a reproducible protocol for the extended normothermic machine perfusion of whole human livers.MethodsHuman livers declined for transplantation were perfused using a blood-based perfusate at 36 °C using the Liver Assist device (XVIVO, Sweden), with continuous veno-venous haemofiltration in-parallel. We developed the protocol in a stepwise fashion.ResultsPerfusion techniques utilised included: targeted physiological vascular flows, phosphate replacement (to prevent hypophosphataemia), N-acetylcysteine (to prevent methaemoglobin accumulation), and the utilisation of sodium lactate as both a nutritional source and real-time measure of hepatocyte function. All five human livers perfused with the developed protocol showed preserved function with a median perfusion time of 168 h (range 120–184 h), with preserved viability throughout.ConclusionsLivers can be reproducibly perfused in excess of 120 (range 121–184) hours with evidence of preserved hepatocyte and cholangiocyte function.

Age, Growth, Sex Composition, and Diet of the Burbot, Lota lota, the Only Freshwater Species of the Family Lotidae in the Amur (Heilongjiang) River, Northeast China

Information about the population structure, including the age and sex composition, growth characteristics, and diet of fish, is essential for the conservation and sustainable exploitation of fish stock. The burbot, Lota lota, is the only freshwater species of the family Lotidae in the Amur (Heilongjiang) River catchment located in northeastern China. Information on the biological characteristics and data on the population structure of this fish from the Amur River are scarce. To study these factors in burbot, Lota lota, in the Amur River of China, 749 specimens from four sampling areas were taken and analyzed in October 2022, January 2023, and May 2023. The ages of the sampled fish ranged from 1+ to 7+ years. The female/male ratio was 1.04:1, and body length and mass varied from 175 to 595 mm and 73.5 to 1958.7 g, respectively. The length–weight regression parameter b value was estimated as 2.80. The parameters of the von Bertalanffy growth model were L∞ = 596, K = 0.221, and t0 = −0.771 for all sampled fish; L∞ = 625, K = 0.208, and t0 = −0.756 for females; and L∞ = 584, K = 0.219, and t0 = −0.980 for males. The analysis of the stomach contents showed fish to be the major source of nutrition across all areas and sampling periods. In January, the secondary prey of burbots in the Huma reach of the upper Amur River was aquatic insect larvae, while, in the Tongjiang and Fuyuan reaches of the middle Amur River, the secondary prey was shrimp. The mean stomach fullness index and rate of empty stomachs differed with the sampling area, with the greatest proportion of empty stomachs observed in the Luobei reach of the middle Amur River, and the lowest in the Tongjiang and Fuyuan reaches. The mean stomach fullness index showed the opposite trend. In the Huma reach, the contributions of fish to the diet and the mean stomach fullness index were significantly higher in May than in October and January. The study provides the first detailed information on the population age and sex structure, growth patterns, and feeding ecology of burbots from the Amur River, China. The results will aid in formulating management strategies and regulations for local populations of burbots in the Amur River, China.

The Potential Bioactive Components in Fruits Wastes as Value-Added Products from the Fruit Processing Industry: A Review

Fruits are an important source of nutrition as most of them contain sufficient amounts of minerals, vitamins, and antioxidants. However, processing these fruits results in enormous waste, accounting for around 40% of total food output. Fruit processing industry waste includes discarded pods, peels, pulp, stones, and seeds all of which can pose environmental and sustainability challenges. Importance of discovering the potential of fruit processing waste into value added products lies in the fact that the fruit processing industries generates substantial waste, contributing to environmental pollution, greenhouse gas emissions, and resource depletion. The solid waste generated by the fruit processing industry may include toxic biological components that contaminate aqueous media, degrade drinking water quality, and cause gastrointestinal disorders in animals. However, Fruit processing waste or byproducts have the potential to be converted into value-added goods of economic importance like candied peel, oils, pectin, reformed fruit fragments, enzymes, and wine/vinegar candied peel. Converting this waste into valuable products can mitigate these issues, while also creating new revenue streams, jobs, and industries. Furthermore, value-added products from fruit processing waste can enhance food security, nutrition, and sustainability, supporting the United Nations' Sustainable Development Goals (SDGs). Additionally, research in this area can also lead to technological innovations, improved efficiency and scalability, enabling companies to gain a competitive edge in the growing market for sustainable products. This article provides an overview of all common and possible consumer friendly uses of fruit processing wastes and deriving value added products out of it.

Determination of polycyclic aromatic hydrocarbons (pahs) in mothers' milk of kafr el-zayat district, egypt: A case study for the health risk of infants in urban regions.

For babies, mother milk is the primary source of nutrition. Persistent organic pollutants (POPs), including polycyclic aromatic hydrocarbons (PAHs), are released into the environment by the majority of metropolitan areas. The purpose of this study was to quantify the risks to milk consumers by determining the amounts of 14 PAH congeners in 60 milk samples that were taken from the rural Tanta area and the urban Kafr El-Zayat district of Egypt. Comparing the mean PAH content in Kafr El-Zayat samples to the reference zone's equivalent value (0.685µg/g fat), it showed 11.87µg/g fat. There is no probability risk when the hazard quotient (HQ) for babies linked to milk consumption is smaller than unity. Regarding low, medium, and high exposure percentiles, the estimated daily dosage equivalent to benzo[a]pyrene (Bap) revealed the corresponding values for mutagenicity: 9.77E-03, 8.37E-03, and 5.58E-03, and carcinogenicity: 5.13E-03, 4.40E-03, and 2.94E-03. Predicted hazards greater than unity were imposed by such doses. Because this was a first examination for an ancient industrial zone in Egypt, the important information of this work explains the projected non-safety pattern of the breast milk ingested in the Kafr El-Zayat region.

Objective: To investigate the use of biological ensiling of organic residues generated by pectiniculture as a nutritive medium in the production of the microalgae Tetraselmis suecica. Theoretical Framework: Biological ensiling is a process that transforms organic residues into a nutritive source through fermentation. In this context, pectiniculture residues, specifically the soft parts of fan shells, can be recycled and used

Objective: To investigate the use of biological ensiling of organic residues generated by pectiniculture as a nutritive medium in the production of the microalgae Tetraselmis suecica. Theoretical Framework: Biological ensiling is a process that transforms organic residues into a nutritive source through fermentation. In this context, pectiniculture residues, specifically the soft parts of fan shells, can be recycled and used as alternative media. Microalgae, such as T. suecica, are photosynthetic organisms that require nutrients for their growth, and their cultivation can benefit from these residues. Method: Twelve vertical bioreactors were used with three experimental treatments (T1, T2, T3) and treatments, one control (CT), with three replicates per treatment. The microalgae were inoculated at a density of 70 x 104 cells mL-1 in a culture volume of 80 L. Cultures were grown in open air with constant aeration. 0-, 60-, 70-, and 80-mL L-1 of the biological silage of fan shell soft parts (EBCA) were dosed. The silage was also chemically characterized and the growth of the microalgae and its relationship with temperature, salinity and pH were determined. Results and Discussion: The results showed that the dosage of 70 mL L-1 of EBCA in T. suecica cultures favors the growth of the microalgae. A significant increase in biomass and cell density was observed compared to the other treatments, indicating that this concentration of silage provides a suitable nutrient medium. The study also analyzed how temperature, salinity and pH conditions affect the growth of T. suecica, finding that certain optimal ranges of these factors can maximize biomass production. Research Implications: Our study demonstrates that pectin culture waste can be effectively recycled as a culture medium for T. suecica production, offering a sustainable and environmentally friendly solution for waste management. This could reduce reliance on traditional culture media and promote more sustainable practices in aquaculture and the microalgal industry. Originality/Value: This study stands out for its innovative approach in the use of organic waste from pectiniculture, such as the soft parts of the fan shell, for the generation of an alternative nutrient medium for microalgae. In addition, large volumes of fan shell wastes are currently being generated, so our work contributes to the management of these wastes.

Thermal evaluation of solar dryers and the effect on the antioxidant and some physicochemical properties of Agastache mexicana and Rosmarinus officinalis.

Lemon balm (Agastache mexicana) and rosemary (Rosmarinus officinalis) were dried using a mixed-mode solar dryer. A 23-factorial design was carried out: operation mode (mesh shade and direct irradiance), airflow (natural convection and forced convection), and type of flat plate solar collector cover (polycarbonate and glass). The drying kinetics of lemon balm and rosemary ranged from 4.5 to 6.5h and 4 to 7h, respectively, according to the operation mode of the solar dryer. The highest percentage of antioxidant activity in the 2,2-diphenyl-1-picrylhydrazil tests was lemon balm, with values between 88% and 93% (100µg/mL), and rosemary, 88% and 92% (100µg/mL). When forced convection was applied, solar collector thermal efficiency increased from 3.97%-5.11% to 17.20%-24.75%, dryer efficiencies ranged from 4.78% to 6.05%, and drying efficiencies between 14.17% and 44.23%. The lowest color difference (6.01) for lemon balm was shown with the mesh shade, forced convection, and glass cover collector, and for rosemary, the lowest color difference (12.87) resulted from using the mesh shade and natural convection; according to the analysis of variance, the cover collector did not affect significantly the color difference. Dehydrated lemon balm and rosemary could be used as an additive for medicinal, gastronomic, and food preservative applications. PRACTICAL APPLICATION: The results are significant for designing dryers for community centers in areas where the production and losses of fruits, vegetables, aromatic herbs, and edible flowers are high. The novelty of this research is to introduce a new product for the consumer; in this case, the traditional dried rosemary and lemon balm in powder form as a nutritional source, both for the preparation of remedies and for direct consumption in dishes, powders, or healthy pills and food supplements.

Historical aspects of the use of goat’s milk as a food product for young children: a review

Goat milk (GM) has been a valuable food product for children and adults all over the world for thousands of years and has been used as an important element of healthy nutrition and healing. The article provides information confirming the importance of GM, which is described in the studies of ancient greek and roman scientists (Hippocrates, Galen), in traditional chinese and arabic medicine (the works of Li Shizheng and Avicenna). The works of domestic and foreign scientists also show the possibility of using GM not only as an alternative to breast milk, but also as a highly effective means for strengthening health. The functional composition of GM makes it possible to create mixtures as close as possible to breast milk, which can be used as a source of nutrition for children in the absence of breastfeeding.

Knowledge, Attitudes, and Practices Regarding Exclusive Breastfeeding among Lactating Mothers in Rural Bangladesh

Background: Exclusive breastfeeding stands at the forefront of maternal and child health, serving as a fundamental pillar for ensuring the optimal growth and development of infants. It not only acts as a crucial source of nutrition but also plays a pivotal role in fortifying the physical and cognitive well-being of newborns, while simultaneously fostering a profound bond between mothers and their infants.Objective: This research seeks to gauge the level of awareness among lactating mothers regarding the significance and benefits of exclusive breastfeeding. It aims to comprehend their attitudes towards exclusive breastfeeding (EBF) and scrutinize their actual practices in implementing this vital aspect of infant care.Materials and Methods: A cross-sectional study was conducted at the Department of Community Medicine, ASWMC, Jashore, in 2018. Data collection took place in the Outpatient Department (OPD) of 'Ad-din Sakina Medical College Hospital' and 'Mother & Child Welfare Centre, Jashore.Results: The study demonstrates a high awareness and adherence to exclusive breastfeeding, with 94.4% aware, 90.9% knowledgeable about recommended duration, 49.9% recognizing advantages, 14.6% aware of disadvantages, and a substantial majority (92.5%) practicing exclusive breastfeeding, though 7.5% do not.Conclusion: Bridging knowledge gaps, cultivating positive attitudes, and promoting consistent breastfeeding practices emerge as crucial components of a comprehensive strategy to boost exclusive breastfeeding rates. The collaboration between healthcare providers, policymakers, and the community is indispensable for the success of such initiatives, ultimately contributing to the overall health and well-being of both infants and lactating mothers. KYAMC Journal Volume: 15, No: 01, April 2024: 46-49.

Fatty acids from nutrition sources for preterm infants and their effect on plasma fatty acid profiles

BackgroundIn preterm infants, IV administration of fat is less well tolerated compared to intake via the enteral route, often resulting in hypertriglyceridemia. It is therefore recommended that parenteral fat intake should not exceed 3.5 to 4.0 g/kg/d whereas human milk can provide up to 8 g/kg/d. It is unknown whether such hypertriglyceridemic conditions are caused by a uniform increase of all fatty acids or it is linked to an elevation of distinct fatty acids due to an unbalanced intake. Obviously, both scenarios could potentially influence the formulation of novel lipid solutions for preterm infants. Objective of this exploratory study was to compare fatty acid profiles between a) different nutritional sources and corresponding plasma samples, b) plasma of infants fed breast milk versus those receiving lipid emulsion, and c) plasma of infants with normal versus elevated triglyceride levels.MethodsForty-seven preterm infants < 36 weeks of gestation were included; fatty acid profiles were measured in serum samples and corresponding nutritional sources (breast milk and lipid emulsion) using gas chromatography/mass spectrometry.ResultsCompared to breast milk levels, plasma contained significantly lower C8:0, C10:0, C12:0, C14:0, C19:1n9, C18:3n3 (p < 0.0001). In contrast, relative abundance of C16:0, C18:0 and C20:4n6 was higher in plasma than in corresponding breast milk samples (p < 0.001) and lipid emulsion (p < 0.01). Compared to the corresponding lipid emulsion, the abundance of C18:2n6 and C18:3n3 was significantly lower in plasma (p < 0.001). Fatty acid profiles in plasma of infants fed breast milk compared to lipid emulsion were not markedly different. Hypertriglyceridemic samples showed elevated levels for C18:1n9 and C16:0 when compared with normotriglyceridemic samples.ConclusionsOur study reveals that lipid levels in plasma show both depletion and enrichment of distinct fatty acids which do not seem to be closely related to dietary intake. A more detailed understanding of fatty acid flux rates is needed, like the understanding of amino acid metabolism and is supported by the finding that hypertriglyceridemia might be a state of selective fatty acid accumulation. This would allow to develop more balanced diets for intensive care and potentially improve clinical outcomes.

L-asparaginase-driven antibiosis in Pseudomonas fluorescens EK007: A promising biocontrol strategy against fire blight

Fire blight, caused by Erwinia amylovora, is a destructive bacterial disease affecting pear and apple trees. The biocontrol ability of Pseudomonas fluorescens EK007 suppresses E. amylovora through competitive exclusion. In this study, EK007 was isolated from the pear phylloplane and characterized as an effective biological agent through antibacterial compounds. To identify the mechanisms underlying EK007's biocontrol activity, physiological tests, transposon insertion mutant libraries, allelic exchange, and whole-genome sequencing were performed. A transposon mutation in the massC homolog gene, part of the massetolide A lipopeptide biosynthesis cluster, reduced the biocontrol efficiency. Allelic exchange confirmed cyclic lipopeptide (CLP) as part of the mechanism. Additionally, a gacA mutant isolated by transposon mutagenesis showed deficient inhibition activity. Culture conditions and nutritional sources clearly influenced EK007's antimicrobial activity against E. amylovora. Growth yield generally correlated with antibiotic production, with amino acids and iron affecting production. Asparagine and aspartate shut down biocontrol activity. This study presents preliminary findings on a novel CLP that may contribute to EK007's antibacterial activity against E. amylovora. While EK007 shows promise as a biocontrol candidate compared to related strains, these results are based solely on in vitro studies, highlighting the need for further investigations to evaluate its efficacy in natural environments.

The Antioxidant Effects of Trypsin-Hydrolysate Derived from Abalone Viscera and Fishery By-Products, and the Angiotensin-I Converting Enzyme (ACE) Inhibitory Activity of Its Purified Bioactive Peptides.

Abalone is a rich source of nutrition, the viscera of which are discarded as by-product during processing. This study explored the biological activities of peptides derived from abalone viscera (AV). Trypsin-hydrolysate of AV (TAV) was purified into three fractions using a Sephadex G-10 column. Nine bioactive peptides (VAR, NYER, LGPY, VTPGLQY, QFPVGR, LGEW, QLQFPVGR, LDW, and NLGEW) derived from TAV-F2 were sequenced. LGPY, VTPGLQY, LGEW, LDW, and NLGEW exhibited antioxidant properties, with IC50 values of 0.213, 0.297, 0.289, 0.363, and 0.303 mg/mL, respectively. In vitro analysis determined that the peptides VAR, NYER, VTPGLQY, QFPVGR, LGEW, QLQFPVGR, and NLGEW inhibited ACE, with IC50 values of 0.104, 0.107, 0.023, 0.023, 0.165, 0.004, and 0.146 mg/mL, respectively. The binding interactions of ACE-bioactive peptide complexes were investigated using docking analysis with the ZDCOK server. VTPGLQT interacted with HIS513 and TYR523, and QLQFPVGR interacted with HIS353, ALA354, GLU384, HIS513, and TYR523, contributing to the inhibition of ACE activity. They also interacted with amino acids that contribute to stability by binding to zinc ions. QFPVGR may form complexes with ACE surface sites, suggesting indirect inhibition. These results indicate that AV is a potential source of bioactive peptides with dual antioxidant and anti-hypertensive dual effects.

Heavy Metal Nanoparticle Detection in Human and Formula Milk.

Breast milk is the natural source of nutrition for infants, but while it supports their health, it can also be a potential source of toxic inorganic particulate matter, and this applies to both breast milk and industrially produced milk. The aim of the present study was to evaluate the presence of nanoparticles in both breast milk and formula milk samples. We collected and analyzed, via a new electron scanning microscopic procedure, 19 samples of breast milk from Italian women and 19 formula milk samples produced by different companies. Organic-inorganic agglomerates were detected in 58% of formula and in 63% of breast milk samples, respectively. In addition, a significantly (p < 0.05) greater size of nanoparticles was observed in formula milk samples. The results, showing the presence of inorganic nanosized particles in breast and artificial milk, may lead to future studies aimed at investigating possible nanosized contamination of milk and identifying early prevention strategies for women and animals involved in the food chain.

Exposure to bisphenols, parabens, and benzophenones in colostrum breast milk of Panamanian women: A pilot study from the PA-MAMI cohort

BackgroundBreast milk is the optimal source of nutrition for infants but can also expose them to endocrine-disrupting chemicals (EDCs), among other environmental contaminants. AimTo determine concentrations of non-persistent phenolic EDCs (three bisphenols, four parabens [PBs], and six benzophenones [BPs]), in colostrum samples from Panamanian mothers and to examine associated reproductive, sociodemographic, and life-style factors. MethodsDispersive liquid–liquid microextraction was used to measure concentrations of bisphenol A (BPA), bisphenol F (BPF), bisphenol S (BPS), methyl- (MeP), ethyl- (EtP), propyl- (n-PrP), and butyl-paraben (n-BuP), and benzophenones BP-1, BP-2, BP-3, BP-6, BP-8, and 4-hydroxy-BP in colostrum milk samples from 36 mothers. An ad hoc questionnaire was used to collect data on potential influentially variables, and multiple linear and logistic regression analyses were conducted. ResultsTwo or more tested EDCs were detected in 36 colostrum samples (100 %), at least four in 14 samples (38.9 %), and at least six in 4 samples (11.1 %). The most frequently detected compounds were BPA (91.7 %), BP-8 (63.9 %), MeP (47.2 %), and BPF (41.7 %). The median concentration was 3.45 ng/mL for BP-8 and 1.37 ng/mL for BPA. No concentrations of n-PrP, BP-1, BP-6, or 4-hydroxy-BP were detected. Associations were observed between phenolic EDC concentrations and maternal place of residence, consumption frequency of poultry, fish, fresh cheese, fruit, yogurt and chocolate, intake of nutritional supplements, and application of some personal care products. ConclusionsBisphenols, parabens, and benzophenones were widely present in colostrum milk samples from Panamanian women. Preventive measures are needed to maximize the benefits of breastfeeding.

Household Food Insecurity Is Associated with Parental Perceptions of and Student Participation in School Meals.

School meals are an important source of nutrition for children and have been found to help mitigate food insecurity. This study evaluated the association between food insecurity and school meal participation and whether parental perceptions about school meals differ by food security status. In May 2022, 1110 Californian parents of K-12 students shared their perceptions about school meals, including meal quality, healthiness, stigma, and benefits, as well as their child's participation in school meals, in an online survey. Household food security was determined using the USDA 6-item module. Logistic and Poisson regression models were used for analysis. The prevalence of household food insecurity was 56.2% (69.6% in households of students eligible for free meals, 55.9% in reduced-price, and 38.3% in non-eligible). Many of the reported benefits of school meals (saving families money and time) were equally highly endorsed by parents with and without food insecurity (p > 0.05). Parents reporting food insecurity had less favorable perceptions of school meals and perceived more stigma (p < 0.05). Food insecurity was positively associated with breakfast participation, especially among elementary school students and students not eligible for free or reduced-price meals (FRPMs) (p < 0.05). Food insecurity is prevalent among California families with school-age children, even in families not eligible for federal FRPMs. Food-insecure households have more negative perceptions of school meals and experience more stigma, though they also report higher breakfast participation. Improving school meal quality and appeal, ensuring parents are familiar with meal quality and healthfulness, and reducing stigma may ease food insecurity while improving children's health.

The cadmium content in commercially key species of seafood from Shandong, China: Potential human health risk estimation

Seafood serves as a vital protein source for human nutrition, yet it also significantly contributes to the intake of dietary cadmium. In this study, we measured the cadmium content in 274 samples from 20 species, including fish, shrimp, and bivalves collected across five cities in Shandong province and assessed the associated health risks for local residents with varying consumption habits. The findings revealed a distinct increasing trend in cadmium concentrations from pelagic fish to benthic bivalve species; however, no evidence of biomagnification was observed among the studied species. Health risk assessments indicated that while there were no non-carcinogenic health risks posed by seafood consumption for local residents exposed to cadmium, carcinogen risks exceeded acceptable levels for lifetime cancer risk. Furthermore, children exhibited approximately twice as high risk indices compared to adults. Bivalve consumption emerged as the primary contributor to these health risks.

Maternal Mediterranean Diet During Lactation and Infant Growth.

Background: Human milk is considered the optimal source of nutrition for infants. Maternal diet is associated with the composition of human milk. The Mediterranean diet (MedDiet) has been studied in pregnancy and during lactation, and it has been associated with changes in milk composition, yet there is a lack of research on MedDiet during lactation and infant outcomes. Methods: Mother-infant dyads (n = 167) from ABC Baby, a prospective observational study, were included in this analysis. Maternal diet was obtained using an adapted version of the National Cancer Institute Diet History Questionnaire II, at 2 weeks or 2 months postpartum. Maternal MedDiet score was calculated using servings of vegetables, fruits, whole grains, nuts and seeds, legumes, fish, monounsaturated-to-saturated fatty acid ratio, red and processed meats, and added sugar. Infants' length, weight, and flank skinfold thickness were measured at 6 months. Using World Health Organization standards, weight-for-age (WAZ), length-for-age (LAZ), and weight-for-length (WLZ) Z-scores were calculated. Multiple linear regression models were adjusted for potential confounders. Results: Higher maternal MedDiet score and intake of fruit and fish were associated with lower flank skinfold thickness (β = -0.33, -0.52, and -1.26, respectively). Intake of nuts and seeds was associated with higher WLZ (β = 0.29). Intake of red and processed meats was associated with lower WAZ (β = -0.18) and LAZ (β = -0.18). Energy-adjusted added sugar intake was associated with lower WLZ (β = -0.02). Conclusions: The maternal MedDiet score was associated with lower skinfold thickness, while its components were associated with differences in anthropometric Z-scores. Further research on the maternal MedDiet and corresponding human milk composition is needed to explore this relationship.

The Relationship Between Mothers' Knowledge and Attitude in Breastfeeding and The Occurrence of Nipple Irritation in Pidie Regency

Research indicates that 40% of women do not breastfeed their infants due to experiencing breast pain and swelling, and fear of nipple soreness. This may be due to incorrect breastfeeding techniques, which are often overlooked by mothers who lack the proper breastfeeding management knowledge, thus requiring relevant training and education. Proper breastfeeding requires good attachment between the mother's areola and the baby's mouth so the baby can feed effectively and nipple irritation can be avoided. If not adhered to, nipple irritation may occur, causing mothers to be reluctant to breastfeed their infants, which may impact to the child's growth rate. Breast milk is an excellent source of nutrition for infants, as it contains essential nutrients needed for optimal growth and development (Prasetyono, 2012). Data from the Aceh District/City Breastfeeding Coverage in 2022 shows that the highest coverage was in Sabang City, with only 22.43% of the population (6,006 infants). This study used a cross-sectional design where 375 infants were breastfed, and the Slovin formula was applied. Samples were randomly selected from infants at health centers in Pidie District. Researchers used the Chi-square test for data analysis. The findings from this study, derived from bivariate statistical analysis using the Chi-square test with a 95% confidence level, resulted in a p-value of 0.000 (p &lt; 0.05). This indicates that the knowledge variable significantly affects breastfeeding technique. The Chi-square test at a 95% confidence level with p=0.000 (p &lt; 0.05) also shows that attitude has an impact on the mother's ability to breastfeed. The mother's knowledge and attitude significantly influence their breastfeeding practice. The best way to prevent nipple irritation is by educating mothers on proper breastfeeding techniques. Researchers state that improving maternal knowledge and attitudes can enhance behavior, especially in breastfeeding practices, to prevent irritation. Monitoring by health workers is expected to improve maternal knowledge and attitudes and minimize the incidence of nipple irritation.

Biological active compounds from edible mushroom: Effect on hela cells

Background: Mushrooms are unique organisms rich in proteins, carbohydrates, vitamins, and minerals, which allows them to be used not only as a source of nutrition but also in cosmetics and medicine. The fungus P. ostreatus possesses two strong enzymatic complexes – lignin-decaying and cellulose-decaying. Objective: The research aimed to study the antitumor activity of treated mm-wave mycelial extracts of mushrooms on the HeLa cell line. Research on edible mushrooms against tumors is very actual and important for the field of functional food science and bioactive compounds field. Methods: The effect of the fungal culture extract on the cyto-photometric and morphometric indices of the HeLa cell line culture was studied according to the nature of proliferation and transcription of malignant tissues. Using high-resolution liquid chromatography, the amino acid composition of acid-soluble proteins in mycelial extracts of a mushroom culture treated with mm waves was studied. Results: Extracts of P. ostreatus mycelial culture significantly suppressed the growth of proliferative activity of human tumor cultures and completely suppressed the mitotic activity of continuous HeLa cell cultures to 72 hours. Our study revealed a significant increase in glutamic and aspartic acids in mycelial culture extracts treated by mm-waves. Conclusion: Based on HPLC analysis, we suggest polyfunctional peroxidase as the candidate for the role of biologically active compound in the composition of aqueous extracts of the mycelial culture. So, the polyfunctional peroxidase plays an active role in the therapeutic properties of the oyster mushroom. Keywords: anticancer activity, ant-inflammation activity, mm-waves, mushroom`s, mycelial extracts, HPLC of acid-soluble proteins, HeLa cell line.

Poultry Nutrition: Achievement, Challenge, and Strategy

Poultry, a vital economic animal, provide a high-quality protein source for human nutrition. Over the past decade, the poultry industry has witnessed substantial achievements in breeding, precision feeding, and welfare farming. However, there are still many challenges restricting the sustainable development of the poultry industry. First, overly focused breeding strategies on production performance have been shown to induce metabolic diseases in poultry. Second, a lack of robust methods for assessing the nutritional requirements poses a challenge to the practical implementation of precision feeding. Third, antibiotic alternatives and feed safety management remain pressing concerns within the poultry industry. Lastly, environmental pollution and inadequate welfare management in farming have a negative effect on poultry health. Despite numerous proposed strategies and innovative approaches, each faces its own set of strengths and limitations. In this review, we aim to provide a comprehensive understanding of the poultry industry over the past decade, by examining its achievements, challenges, and strategies, to guide its future direction.