Sophorolipid Production Research Articles

Castor oil as secondary carbon source for production of sophorolipids using Starmerella bombicola NRRL Y-17069.

Sophorolipids (SLs), a prominent member of the biosurfactants family are produced in acidic and/or lactonic form by yeast Starmerella bombicola NRRL Y-17069 when grown on hydrophilic or hydrophobic or both carbon sources. In current study, ricinoleic acid rich castor oil (10%) was used as hydrophobic and glycerol (10%) was used as hydrophilic carbon source. The yields of 24.5 ± 0.25 g/l sophorolipids were analyzed by anthrone and HPLC method which further increased upto 40.24 ± 0.76 g/l sophorolipids using fed batch process at 5L fermenter level. The structures of sophorolipids synthesized on castor oil were elucidated by liquid chromatography-mass spectrometer (LC-MS), (13)C and (1)H NMR. The results indicated that the ricinoleic acid (RA) gets hydroxylated at ω-1 position but incorporated into sophorolipids through already available hydroxyl group at 12(th) position. It resulted in the production of a novel sophorolipids with hydroxyl fatty acid as side chain and has applications as surfactant for novel drug delivery, anti microbial agent, cosmetic ingredient and emulsifier.

Sophorolipid production from biomass hydrolysates.

Although extensive research has been conducted on producing sophorolipids using Candida (Starmerella) bombicola from pure sugars and various oil sources, production of this biosurfactant has not been evaluated when cells are cultivated in lignocellulosic hydrolysates. Here, we report for the first time that C. bombicola is capable of producing sophorolipids on hydrolysates derived from sweet sorghum bagasse and corn fiber. Without oil supplementation, a sophorolipid concentration of 3.6 and 1.0 g/L was detected from cultures with bagasse and corn fiber hydrolysates, respectively. With the addition of soybean oil at 100 g/L, the yield of sophorolipids from these two hydrolysates in the same order was 84.6 and 15.6 g/L. Surprisingly, C. bombicola consumed all monomeric sugars and nonsugar compounds in the hydrolysates, and cultures with bagasse hydrolysates had higher yield of sophorolipids than those from a standard medium which contained pure glucose at the same concentration.

Improved synthesis of sophorolipids from waste cooking oil using fed batch approach in the presence of ultrasound

Waste cooking oil (WCO) has been used as a sustainable and economical raw material for the production of sophorolipids using Starmerella bombicola. Initially, pretreatment of waste cooking oil has been carried out using activated earth for reduction of the peroxide value to less than 5. Fermentation experiments were carried out in a fermentor of capacity 2.5L and the effect of stirring speed, presence of ultrasound and mode of operation (i.e. batch or fed batch) on the sophorolipid yield has been investigated. Batch fermentation in the presence of ultrasound gave higher yield of sophorolipids as 24.7gL−1 at a stirring speed of 300rpm as compared to the operation involving only stirring. The better results can be attributed to cavitational effects which increase the cell permeability, improve the substrate intake and increase the metabolism of microbial cells leading to an overall increase in the sophorolipid yield. Fermentation kinetic models have been developed for cell growth, product yield and substrate consumption. Sophorolipid yield was observed to be the maximum as 55.6g/L in the fed-batch mode of fermentation assisted with ultrasound. The obtained sophorolipid was found to contain major component as lactonic sophorolipid, which has been confirmed by FTIR analysis. The surface active properties of the final product have also been confirmed based on the fact that the use of sophorolipid reduced the surface tension of water from 72 to 32.6mN/m and interfacial tension at the water and n-heptane interface in the presence of sophorolipid was 1.4mN/m.

Efficient production of sophorolipids by Starmerella bombicola using a corncob hydrolysate medium

Sophorolipids (SLs) are amphiphilic compounds produced from a variety of saccharides and vegetable oils by the yeast Starmerella bombicola and related strains, and they have commercial uses as detergents. In the present study, SL production was investigated using a corncob hydrolysate (CCH) medium derived from lignocellulosic feedstocks as a source of hydrophilic carbon substrates. Excess sulfuric acid concentrations during pretreatment of the corncobs increased the furfural concentrations and turned the CCH dark brown. The optimal sulfuric acid concentration was 1% (w/v), and the treated CCH, containing 45 g/l glucose, allowed the production of 33.7 g/l of SLs following 4 days of cultivation. Additional autoclaving (121°C, 20 min) inhibited SL production and cell growth by 36% and 40%, respectively. Ammonium nitrate (0.1 g-N/l) restored SL production to the autoclaved CCH. Finally, a cost-effective SL production of 49.2 g/l, with a volumetric productivity of 12.3 g/l/day, was achieved using CCH medium during batch cultivation in a jar fermentor.

Sophorolipids Synthesized Using Non-Traditional Oils with Glycerol and Studies on Their Surfactant Properties with Synthetic Surfactant

Abstract The importances of bio-surfactants in industrial applications are huge due to their biodegradable and eco-friendly nature. Bio-surfactants mostly find application in cosmetics and health care products. Moreover, bio-surfactants like sophorolipids (SL) also exhibit antimicrobial and skin healing properties. The current studies involve production of SL using low cost substrates like glycerol (15%) instead of glucose (10%) with non-traditional oils (10%) such as jatropha oil, karanja oil and neem oil by using Starmerella bombicola (ATCC 22214). Neem oil gave lower yield i.e. (1.42 g/L) of SL as compared to jatropha oil (4.74 g/L) and karanja oil (5.91 g/L) with glycerol as substrate. Some pretreatment given to crude neem oil like oil refining and ethanol washing with glycerol helps in improved cell growth and SL yield i.e. 2.73 g/L and 3.82 g/L respectively. The comparison thin layer chromatography (TLC), Fourier Transform infrared spectra (FTIR), high performance liquid chromatography (HPLC), liquid chromatography mass spectra (LC-MS) and proton nuclear magnetic resonance spectroscopy (1H NMR) of SL produced on non-traditional oils with glycerol were carried out with that of SL produced on non-traditional oils with glucose. The surfactant properties of sodium lauryl sulphate (SLS) such as surface tension, interfacial tension, stabilization of foam, emulsification, and wetting were improved when SLS was replaced at different concentration of SL.

Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y2A CGMCC 3798

Different metal ions were added as supplements to culture media to study sophorolipids production by Wickerhamiella domercqiae Y2A CGMCC 3798. The fermentation media were supplemented with different metal ions in the form of sulfate. A maximum yield 149.90 g/L was achieved in the presence of Cu2+ ions. The production of lactonic sophorolipids was favored when culture media wad supplied with Mg2+ ions, while Fe2+ ions were the optimal ions for acidic sophorolipids production. TLC, HPLC, and LC–MS analyses were used for further investigation on the sophorolipids production. The results of SDS–PAGE and enzymatic transformation of acidic sophorolipids showed differential expression of extracellular proteins of W. domercqiae Y2A CGMCC 3798 in presence of Mg2+ or Fe2+. Overall the results indicated potential selective production of different sophorolipids based on selected metal ions in culture media.Practical applications: During the fermentation process of sophorolipids, L‐SLs and A‐SLs were produced selectively by adding Mg2+ and Fe2+into fermentation media, respectively.Sophorolipid composition and production are different produced by Wickerhamiella domercqiae Y2A CGMCC 3798 cultured with Mg2+ (a) and Fe2+ (b), respectively by HPLC analysis.

Production, characterization and anticancer activity of <em>Candida bombicola</em> sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

The production of sophorolipids by Candida bombicola NRRL Y- 17069 grown in a mixture of sunflower oil cake and crude soybean oil as economic substrates with different fermentation techniques was studied. The highest yield (49.5 g·100 g −1 substrates) was obtained from solid state fermentation after employing a new concept for extraction by methanol (E I) followed by ethyl acetate (E II), then partially purified with hexane (E III). The course of time of fermentation was also studied, and E I extracted of the 12 th day showed the minimum surface tension (45 mN·m −1 ) at a critical micelle dilution (CMD) of 10% concentration. The produced sophorolipids were characterized and confirmed by FTIR and 1 H NMR spectroscopy. The anticancer activity of the produced compounds was assessed against MCF-7, HepG2, A549, HCT116 cancer cell lines and the results revealed that E III and E IV (a mixture of E I & E III) act as promising anticancer agents in HepG2 and A549 by inhibiting urokinase and histone deacetylase activities.

Production of sophorolipids synthesized on castor oil with glucose and glycerol by using Starmerella bombicola (ATCC 22214)

Biosurfactants such as sophorolipids are natural and eco‐friendly surfactants that are used in cosmetics and health care products. In addition to surfactant properties, sophorolipids also possess antimicrobial and skin healing properties. In the present work, castor oil (10%) was used as a non‐conventional carbon source in combination with glucose (10%) or with glycerol (15%) for fermentative production of novel sophorolipids by using Starmerella bombicola (ATCC 22214). The yields of sophorolipids are 6.1 g/L on castor oil with glucose as a carbon source and 2.7 g/L on castor oil with glycerol as a carbon source analyzed by anthrone method and HPLC. The structures of sophorolipids synthesized on castor oil were elucidated by liquid chromatography–mass spectrometry and 1H NMR of the purified compounds. The results indicated the incorporation of ricinoleic acid into sophorolipids without omega‐1 oxygenation step that is required for incorporation of oleic acid into sophorolipids. It resulted in the production of novel sophorolipids that can find various applications as cosmetic ingredients and antimicrobial agents.Practical applications: Castor oil, widely produced in India, contains palmitic acid (1–2%), strearic acid (1–2%), oleic acid (6–8%), linoleic acid (0.5–1%), and mainly ricinoleic acid (80–90%). The presence of hydroxyl fatty acid as ricinoleic acid makes castor oil different from other vegetable oils. Therefore, castor oil is being used as a valuable raw material in various chemicals industries. The present work proved that castor oil can be used as a new substrate for the production of sophorolipids with a novel structure having hydroxyl group on the moiety. The end product can be used for enhancement of surfactant properties in combination with a chemically synthesized surfactant.

Antimicrobial and SEM Studies of Sophorolipids Synthesized Using Lauryl Alcohol

AbstractIn recent years, researchers have developed biosurfactants for industrial, pharmaceutical and medical applications revealing the promising biological activities of these biomolecules. One of the best studied microbial surfactants are glycolipids, especially sophorolipids (SLs) produced by selected non‐pathogenic yeast species of Candida. They are biodegradable, non‐toxic and are environmentally friendly. Sophorolipid production was carried out using glucose as the hydrophilic source and lauryl alcohol C12–14, as the hydrophobic source using Candida bombicola ATCC 22214. Primary characterization of the SL obtained using lauryl alcohol (SLLA) was done by FTIR which depicted the presence of alkyl sophorosides/SLs. Antimicrobial activity testing revealed that SLLA showed complete inhibition against gram negative bacteria, Escherichia coli (ATCC 8739) Pseudomonas aeruginosa (ATCC 9027) at 30 and 1 μg/ml at a contact time of 2 and 4 h respectively. Whereas for gram positive bacteria Staphylococcus aureus (ATCC 6358), Bacillus subtilis (ATCC 6633), complete inhibition was observed at 6 and 1 μg/ml respectively at a contact time of 4 h. The formed SLLA showed noteworthy inhibition against the pathogenic yeast Candida albicans (ATCC 2091) at 50 μg/ml with a contact time of 4 h. These values are remarkably low compared to reported values of oleic acid SLs and linolenic acid SLs which were studied for antimicrobial properties. Scanning electron microscopy analysis of the treated cells revealed the changes in morphology and topography of the microorganisms.

Candida bombicola as a platform organism for the production of tailor‐made biomolecules

The yeast Candida bombicola is capable of producing high amounts (400 g/L) of the biosurfactant sophorolipids. The genetic makeup of this industrially important yeast has recently been uncovered and molecular manipulation techniques have been developed. Hence, all tools for the development of new bioprocesses with C. bombicola are now available. As a proof of concept, the production of two totally different molecules was aimed for: the bioplastic polyhydroxyalkanoate (PHA) and a new-to-nature cellobioselipid-biosurfactant. Integration of the new functionalities at genomic loci necessary for sophorolipid production safeguards the new biomolecules from sophorolipid contamination, while taking advantage of the regulation of the sophorolipid gene cluster. A maximum yield of 2.0% wt/dwt PHA was obtained; furthermore, this is the first time cellobioselipid synthesis by a non-natural producer is reported. We here provided proof of concept that C. bombicola can be transformed into a platform organism for the production of tailor-made biomolecules.

Sophorolipid production from delignined corncob residue by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 and Cryptococcus curvatus ATCC 96219

Delignined corncob residue hydrolysate (DCCRH) and detoxified DCCRH were used for single cell oil (SCO) and single cell protein (SCP) production of Cryptococcus curvatus ATCC 96219 and for sophorolipid (SL) production of Wickerhamiella domercqiae var. sophorolipid CGMCC 1576. Both C. curvatus and W. domercqiae could utilize glucose in DCCRH to grow and accumulate lipids or particle-shaped SLs. DCCRH detoxification by activated carbon adsorption not only improved cell growth and lipid accumulation of C. curvatus but also increased SL production and proportion of lactonic SL in total SL. A total biomass of 17.36g/l with a lipid content of 44.36% could be achieved after cultivation of C. curvatus on the detoxified DCCRH. The predominant fatty acids of the produced SCO were oleic, stearic, and palmitic acids (27.2, 20.5, and 15.7%, respectively). When W. domercqiae cells were cultivated on DCCRH and SCO, total SL production of 39.08g/l (DCCRH + SCO) and 42.06g/l (detoxified DCCRH + SCO) were obtained. Furthermore, when cell lysate of C. curvatus, oleic acid, and DCCRH/detoxified DCCRH was used as nitrogen and carbon sources, total SL production reached 37.19g/l and 48.97g/l, respectively. These results demonstrated that renewable DCCRH can be utilized for the production of high-value SCO and SLs.

The biosynthetic gene cluster for sophorolipids: a biotechnological interesting biosurfactant produced by Starmerella bombicola

Sophorolipids are promising biological derived surfactants or detergents which find application in household cleaning, personal care and cosmetics. They are produced by specific yeast species and among those, Starmerella bombicola (former Candida bombicola) is the most widely used and studied one. Despite the commercial interest in sophorolipids, the biosynthetic pathway of these secondary metabolites remained hitherto partially unsolved. In this manuscript we present the sophorolipid gene cluster consisting of five genes directly involved in sophorolipid synthesis: a cytochrome P450 monooxygenase, two glucosyltransferases, an acetyltransferase and a transporter. It was demonstrated that disabling the first step of the pathway - cytochrome P450 monooxygenase mediated terminal or subterminal hydroxylation of a common fatty acid - results in complete abolishment of sophorolipid production. This phenotype could be complemented by supplying the yeast with hydroxylated fatty acids. On the other hand, knocking out the transporter gene yields mutants still able to secrete sophorolipids, though only at levels of 10% as compared with the wild type, suggesting alternative routes for secretion. Finally, it was proved that hampering sophorolipid production does not affect cell growth or cell viability in laboratory conditions, as can be expected for secondary metabolites.

Production of sophorolipids with eicosapentaenoic acid and docosahexaenoic acid from Wickerhamiella domercqiae var. sophorolipid using fish oil as a hydrophobic carbon source

Sophorolipids (SLs) were synthesized by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 grown on fish oil and glucose. They were purified using preparative HPLC and their structures were identified by MS/MS. The yields of total and lactonic SLs were 47 and 19gl(-1) in shake-flasks when fish oil 4% (v/v) was used with glucose in the medium. The composition of SL mixture contained more than 20 SL molecules. Several unconventional SL molecules with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) including zero-, mono- and di-acetylated acidic SLs with EPA and a di-acetylated acidic SL with DHA were obtained. Two unconventional lactonic SL molecules, non-acetylated lactonic SL with 22:3 and non-acetylated lactonic SL with 20:0, were also obtained.

Production of Sophorolipids from Non-edible Jatropha Oil by Stamerella bombicola NBRC 10243 and Evaluation of their Interfacial Properties

To facilitate the development of bio-based chemicals from renewable and inexpensive natural resources, we sought to produce biosurfactants using non-edible jatropha oil. Twenty yeasts known to produce biosurfactants were tested in this study, and Stamerella bombicola NBRC 10243 was found to use jatropha oil efficiently to produce sophorolipids (SLs) as a mixture of lactone-form SL (L-SL) and acid-form SL (A-SL). Under culture conditions using rice bran as the source of organic nutrients, the yield of SLs reached 122.6 g/L in 5-L jar fermentors after 9 d in culture. HPLC analysis of the culture medium showed that the levels of phorbol esters (PEs), major toxic components of the oil, decreased markedly with an increase in culture time, suggesting that the yeast degrades PEs. Although the SLs obtained by solvent extraction of the culture medium contained a small amount of PEs, the sodium salt of A-SL (A-SL-Na) obtained by alkaline treatment (5N NaOH, 80°C) showed no PE peaks upon HPLC analysis. A-SL-Na had excellent surface activity with low CMC (9.0×10⁻⁴ M) and γ(CMC) (29.6 mN/m), which are lower than that of sodium dodecyl sulfate (SDS). The solubilizing ability of A-SL-Na toward for octanoic acid ([octanoic acid]/[A-SL-Na]) was found to be 2.0, which is half that of SDS. Our findings should help improve SL production from non-edible feedstock and broaden the use of promising bio-based surfactants.

Jatropha Oil Derived Sophorolipids: Production and Characterization as Laundry Detergent Additive

Sophorolipids (SLs) are glycolipidic biosurfactants suitable for various biological and physicochemical applications. The nonedible Jatropha oil has been checked as the alternative raw material for SL synthesis using C. bombicola (ATCC22214). This is useful towards lowering the SL production cost. Through optimization of fermentation parameters and use of resting cell method, the yield 15.25 g/L could be achieved for Jatropha oil derived SL (SLJO) with 1% v/v oil feeding. The synthesized SL displayed good surfactant property. It reduced the surface tension of distilled water from 70.7 mN/m to 33.5 mN/m with the Critical Micelle Concentration (CMC) value of 9.5 mg/L. Keeping the prospective use of the SL in mind, the physicochemical properties were checked along with emulsion stability under temperature, pH stress, and in hard water. Also antibacterial action and stain removal capability in comparison with commercial detergent was demonstrated. SLJO enhanced the detergent performance. Based on the results, it can be said that SLs have utility as fabric cleaner with advantageous properties such as skin friendly nature, antibacterial action, and biodegradability. Therefore SLs are potential green molecules to replace synthetic surfactants in detergents so as to reduce harm caused to environment through detergent usage.

Design of selective production of sophorolipids by Rhodotorula bogoriensis through nutritional requirements

Rhodotorula bogoriensis is known as the producer of longer chain acidic sophorolipids (SLs) with a unique hydroxylation position where the sophorose unit is linked to the 13-hydroxydocosanoic acid. The influence of initial inoculum concentration, hydrophilic and hydrophobic carbon, and nitrogen sources on R. bogoriensis growth and SL production was evaluated to obtain a selective SL production. Experiments took place in microtiter plates, used as minireactors, after the verification of its suitability compared with shake flasks. The common structure of SLs is the 13-[2'-O-β-d-glucopyranosyl-β-d-glucopyranosyloxy]-docosanoic acid SL. The analysis of the fermentation media using high-performance liquid chromatography with evaporative light scattering detector showed the production of four main SLs, respectively, in the following forms: (i) deacetylated (peak A) (C22:0 SL), (ii) 6″monoacetylated (peak B) (C22:0-6″Ac SL), (iii) 6'monoacetylated (peak C) (C22:0-6'Ac SL), and (iv) 6',6″ diacetylated (peak D) (C22:0-6',6″Ac SL). The identification of compounds in SL mixtures was performed by liquid chromatography with electrospray ionization mass spectrometry analysis, and no differences were observed. Besides the four compounds detected using high-performance liquid chromatography with evaporative light scattering detector chromatograms, three other SLs was identified, corresponding to mono- and diacetylated C24:0 hydroxy fatty acid SLs. To our knowledge, this work presents for the first time the production and identification of C24:0 SLs. A longer hydrophobic tail on SLs had an important role in the improvement of surface active properties. The selection of a specific time for fermentation end and the use of different carbon (e.g. glucose, fructose, mannose, lactose, galactose, xylose) and nitrogen (e.g. peptone, (NH(4))(2)SO(4) and NaNO(3)) sources led to a selective production of de-, mono-, and diacetylated SLs by R. bogoriensis.

Sophorolipids: improvement of the selective production by Starmerella bombicola through the design of nutritional requirements

Microtiter plates were used as minireactors to study Starmerella bombicola growth and sophorolipid (SL) production. Compositional analysis of SL mixtures by liquid chromatography with electrospray ionization tandem mass spectrometry showed similar results on SLs produced using the laboratory scale (shake flask) and the microscale (24-well microtiter plates (MTP)) approach. MTP suitability on SL production was proven, being this approach, especially advantageous on SL screening. Several hydrophilic carbon sources, hydrophobic co-substrates and nitrogen sources were supplied to culture media, and their influence on SL production was evaluated. The selection of specific hydrophobic co-substrate and nitrogen sources influenced the ratio acidic/lactonic SLs. In fact, it was observed that the production of acidic C18:1 diacetylated hydroxy fatty acid SLs was favoured when culture media was supplied with avocado, argan, sweet almond and jojoba oil or when NaNO(3) was supplied instead of urea. This last case was observed after 144 h of cultivation. A new SL, lactonic C18:3 hydroxy fatty acid diacetylated SL, was detected when borage and onagra oils were used individually as co-substrates. Overall results indicated the potential of the selective production of different and new sophorolipids by Starmerella bombicola based on the selection of carbon and nitrogen sources to culture media.

Production of sophorolipids with enhanced volumetric productivity by means of high cell density fermentation

To achieve high time-space efficiency for sophorolipid production with yeast Candida bombicola, a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal cell density and (2) the computer-aided adjustment of physical parameters and the controlled feeding of substrates for enhanced volumetric productivity. Both stages have been successfully implemented in a 10-L fermenter, where up to 80g dry cell weight/L was obtained and a remarkably high volumetric productivity (> 200g isolated sophorolipids/L/day) was achieved. Both the biomass and volumetric productivity were markedly higher than previously reported. Specifically, the high productivity of sophorolipids could be attained on a very short time scale (24h), highlighting the industrial potential of the platform developed in this work.

Recovery of purified lactonic sophorolipids by spontaneous crystallization during the fermentation of sugarcane molasses with Candida albicans O-13-1

Numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. These studies have not yet obtained satisfied results in combining a low-cost fermentation process and the purification of lactonic sophorolipids. This study establishes a fed-batch fermentation process of purifying sophorolipids from Candida albicans O-13-1 using low-cost sugarcane molasses as the substrate. In the optimized conditions of this research, using sugarcane molasses as a substrate and product synthesis based on the temperature stage-controlled fermentation, our result indicates that sophorolipids production could reach 108.7g/L. More importantly, lactonic sophorolipids can crystallize and precipitate during our established fermentation process. The structures and content of sophorolipids separated from the fermentation broth and sophorolipids crystallized in the fermentation broth were analyzed by a scanning electron microscope (SEM) and liquid chromatography–mass spectrometry (LC–MS). The fermentation process produced 90.5g/L crystallized lactonic sophorolipids with 90.51% purity. This is an energy-saving and low-cost method to obtain such pure lactonic sophorolipids.

Microbial Oxidation of Medium Chain Fatty Alcohol in the Synthesis of Sophorolipids by Candida bombicola and its Physicochemical Characterization

AbstractSophorolipids (SL) are glycolipid biosurfactants abundantly produced from different feedstocks by yeasts and have been widely developed for various applications. The amphipathic structures of sophorolipids imparts to them surfactant type properties. These biosurfactants are readily isolated in high yield and are nontoxic and biodegradable, which make them industrially interesting as surfactants or emulsifiers. Sophorolipid production was carried out using glucose as a hydrophilic source and lauryl alcohol C12–14, as a hydrophobic source by Candida bombicola (ATCC 22214). Process economics and environmental attractiveness was increased by using a low cost substrate. Optimization studies were carried out where the effect of glucose concentration, pH, temperature and metal ions on sophorolipid production was studied. Optimum production of sophorolipid obtained using lauryl alcohol (SLLA) was achieved after 96 h, the initial pH of the medium was 6.0 with 10 % glucose at 30 °C. Primary characterization of SLLA was done by FTIR. The SLLA showed high physicochemical properties in terms of the surface activities when compared with synthetic surfactants including dodecyl tetraethylene glycol ether and sodium lauryl sulfate. The surface tension of SLLA thus obtained was found to be 24 mN/m with a critical micellar concentration (CMC) value of 0.68 mg/L. Antimicrobial testing showed that SLLA have potent antimicrobial activity against both gram negative bacteria, Escherichia coli (ATCC 8739) and gram positive cocci, Staphylococcus aureuss (ATCC 2079).