We compared haploidization rates and preimplantation development of embryos generated by somatic cell nuclear transfer using three sources of somatic cells: cumulus oophorus, fibroblasts, or mouse embryonic stem cells (mESCs). Metaphase II (MII) enucleated oocytes isolated from B6D2F1 mice were fused with cumulus cells (CCs), fibroblasts, or mESCs. The resulting constructs, identifiable by meiotic-like spindle development, were injected with mouse spermatozoa by piezo-ICSI. A series of unmanipulated oocytes were piezo-ICSI inseminated and served as controls. Successful haploidization by extrusion of a pseudo polar body, the appearance of biparental pronuclei, and full preimplantation development were compared between the experimental and control groups. Whole genome karyotype analysis was performed on the resulting blastocysts. MII oocytes were enucleated in the presence of cytochalasin B by extracting the spindle complex and first polar body under Oosight™ visualization. A single CC, fibroblast, or mESC was fused with the ooplast of enucleated oocytes using Sendai virus. After ≥2 hours, successful somatic cell fusion and meiotic-like spindle development were assessed. Piezo-actuated sperm injection was executed on the experimental and unmanipulated oocyte controls. Fertilization and preimplantation development were monitored by time-lapse imaging. DNA seq was used to assess the karyotype of the resulting blastocysts. After enucleation of 535 MII oocytes, haploidization by spindle development was verified at a significantly higher rate for those transferred with CCs (56%, P< 0.00001) compared to those with fibroblasts (30%) or mESCs (32%). After injection, the controls had an 86% survival rate, while oocytes reconstituted with CCs, fibroblasts, or mESCs survived at rates of 54%, 53%, and 57%, respectively (P<0.001). The control group fertilized at a rate of 74%, while oocytes reconstituted using CCs fertilized at a rate of 52%, fibroblasts at 35%, and mESCs at 55% (P<0.001). Finally, 81% of fertilized oocyte controls developed to blastocysts, while only 21% of oocytes reconstituted with CCs, 20% of fibroblasts, and 5% of mESCs reached the blastocyst stage (P< 0.00001). Moreover, experimental conceptuses had morphokinetic developmental characteristics to the blastocyst stage comparable to the controls, as assessed by time-lapse imaging. Preliminary results of 4-8 cells taken from 9 CC-derived blastocysts and 1 mESC-derived blastocyst, sequenced at an independent laboratory, show that 5 CC blastocysts and the 1 mESC blastocyst were euploid. While these results indicate that the chances of fertilization and blastocyst development among the different somatic cell sources are lower than the controls, they were still achievable and had comparable morphokinetic characteristics. CCs also appear to be more easily reprogrammed, as shown by a significantly greater rate of haploidization. Once optimized and reproduced in humans, this technique may generate genotyped female gametes to alleviate age-related infertility.