Solvent-extracted Soybean Meal Research Articles

103 Effects of partial or total replacement of dried distiller’s grain with soybean meal on growth performance, carcass characteristics, sera urea nitrogen, and net energy utilization in finishing beef steers

Abstract The objective of this study was to determine if partial or complete replacement of dried distiller’s grains plus solubles (DDGS) with solvent-extracted soybean meal (SBM) influences growth performance, efficiency of dietary net energy utilization, sera urea-N (SUN) concentrations, or carcass traits in finishing beef steers. Continental × British steers [initial shrunk body weight (BW) = 382 ± 37 kg] were used in a 139-d experiment. Treatments included: DDGS fed at 20% DM (13.4% CP; DDGS), SBM replacing 50% of DDGS (14.4% CP; SBM50), and SBM replacing 100% of DDGS (15.4% CP; SBM100). Steers (n = 189) were weighed and allotted to 1 of 24 pens (n = 8 steers per pen; 8 pens per treatment) at the Ruminant Nutrition Center near Brookings, SD. Whole blood was collected and harvested as sera on d 77, 105, and 139 to determine SUN concentration. Growth and carcass data were analyzed as a randomized complete block design using the GLIMMIX procedure of SAS 9.4 with treatment and block (initial BW grouping) as fixed effects; SUN data were analyzed using repeated measures, pen was the experimental unit. Inclusion level was evaluated for linear and quadratic components by the method of orthogonal polynomials. Final BW was calculated by dividing hot carcass weight (HCW) by 0.625. No differences were noted (P ≥ 0.18) for final BW, average daily gain, dry matter intake, or feed efficiency. Dietary treatment tended to affect gain to feed ratio (G:F; quadratic; P = 0.10) and SBM increased the apparent efficiency of energy capture. Treatment had no effect (P ≥ 0.22) on HCW, rib fat, marbling score, USDA yield grade, or final BW adjusted to 28% empty body fat. Complete replacement of DDGS with SBM increased rib eye area by 1% (linear; P = 0.02), but decreased dressing percentage (linear, P = 0.03). Distribution of USDA quality or yield grades were unaffected by treatment (P ≥ 0.36). No treatment × day interaction was observed for SUN (P = 0.20). However, day (P < 0.01) and treatment (P < 0.01) effects were observed. Initial samples (d 77) differed between DDGS and SBM treatments, with the latter having greater SUN values (P < 0.01). On d 105, the SBM100 treatment had greater SUN than DDGS, and SBM50 was intermediate (P < 0.01). On d 139, steers from SBM100 had elevated increased SUN compared with SBM50 and DDGS treatments (P < 0.01). Net energy values calculated from animal performance for DDGS agreed closely with tabular estimates. In this experiment, replacement of DDGS with SBM increased the observed to expected ratio of dietary NE, increased muscularity and tended to decrease DP with no other observed effects on growth performance or carcass traits.

156 Growth performance, energy, and nutrient digestibility of nursery pigs fed high-shear dry extruded corn and soymeal

Abstract Altering the processing method via high-shear dry extrusion of both soybean meal and corn has been shown to improve diet energy and digestibility. The objective was to evaluate the effect using high-shear dry extruded corn and mechanically processed soymeal on growth performance, energy, and nutrient digestibility of nursery pigs. A total of 240 newly weaned pigs [initial body weight (BW) = 5.7 ± 1.3 kg] were assigned to 1 of 5 dietary treatments (n = 16 pens per treatment with 3 pigs per pen) in a complete randomized design for three dietary phases over a 6 wk study duration. Pigs were allowed a 7-d acclimation period where they were fed a common commercial medicated starter pellet diet (d 0-7, Phase 1). In phase 2 and 3, experimental diets included: 1) solvent-extracted soybean meal and conventional ground corn (Con), 2) solvent-extracted soybean meal and high-shear dry extruded corn (ECorn), 3) mechanically processed soymeal and conventional corn (ExSoy), 4) mechanically processed soymeal and high-shear dry extruded corn (same ME and Lysine as treatment 1 to 3; Comb), and 5) mechanically processed soymeal and conventional corn (energy floated with same Lys:ME; Comb+). The phase 2 mash test diets contained 0.40% titanium dioxide as a digestibility marker (d 8-21) and phase 3 test diets were fed from d 22-42. Pigs were individually weighed on d 0, 7, 21 and 42 and feed disappearance was recorded to calculate average daily feed intake (ADFI) and Gain:Feed within phase 1, 2 and 3, and overall (phase 2 and 3 combined). Data were analyzed using pen as the experimental unit. Compared with Cont, end BW did not differ in the ECorn and ExSoy treatment, while Comb and Comb+ pigs were 13 to 17% lighter (26.1, 24.2, 24.0, 22.6 and 21.5 kg, respectively, P = 0.004). Overall, the Con fed pigs had 30% greater average daily gain (ADG) and 12 to 21% increase in feed efficiency compared with either Comb or Comb+ treatments (P < 0.001). Overall ADFI did not differ. During phase 3, Con and ECorn fed pigs observed increased ADG and feed efficiency compared with pigs fed either Comb treatment (Both P = 0.002, respectively). Compared with Cont, ECorn and Comb+ had greater GE ATTD coefficients, while ExSoy and Comb were less (82.1, 84.6, 86.9, 78.7 and 79.1%, respectively, P < 0.001). Nitrogen ATTD coefficients were greatest in Comb+, followed by ECorn, Cont, ExSoy and Comb (84.4, 78.8, 77.9, 73.0 and 72.3%, respectively, P < 0.001). In conclusion, substituting conventional corn or solvent-extracted soybean meal with extruded corn or mechanically processed soymeal resulted in equal pig growth performance. Further research is needed to determine the effects on the performance of nursery pigs fed diets including more than one high-shear dry extruded ingredients.

PSI-5 Evaluation of EhV (surrogate for African swine fever virus) inactivation in corn- and soybean-based feed ingredients and diets at various times and temperatures during storage

Abstract African swine fever virus (ASFV) is a member of the nucleocytoplasmic large DNA viruses (NCLDVs) and remains stable in a variety of environments, including animal feed ingredients as shown in previous laboratory experiments and simulations. Emiliania huxleyi virus (EhV) is another member of NCLDV, which has a restricted host range limited to a species of marine algae called Emiliania huxleyi. This algal NCLDV has many similar morphological and physical characteristics to ASFV which makes it a safe and reliable surrogate for studying ASFV survival and inactivation in feed ingredient matrices in-situ under various time and temperature storage conditions. The objective of the present study was to investigate the inactivation of ASFV in various feedstuffs under a wide range of time and temperature conditions during storage using EhV as a surrogate. Corn- and soybean-based feed ingredients including corn, corn fermented protein, distiller dried grains with solubles, extruded soybean meal, high protein distiller’s grains, solvent-extracted soybean meal, soybean hulls, complete feed, and a positive control (no matrix) were inoculated with EhV (1 × 108 viruses/mL). Negative controls were inoculated with medium containing no EhV for each matrix. To assess the effects of different times and temperatures of storage, all samples were stored at temperatures of 4, 24, and 34 °C for 1, 5, 60, and 120 d. For each timepoint and temperature combination, samples were evaluated for virus presence (qPCR) and viability using a previously validated viability qPCR (v-qPCR) method. On average, we observed a 0.2 log reduction in viable viral DNA content over the 120-d period, with a 0.1 log reduction across varying temperatures. No reductions in EhV viability exceeding 1 log were detected for any matrix and the positive control at any time and temperature combination evaluated. No EhV was recovered from the negative control samples. Given that the experimental error was calculated to be within the range of 1 log, we concluded that storing EhV at 4, 24, or 34 °C for up to 120 d did not result in the inactivation of EhV in any of the ingredients, complete feed, or the control with no matrix. These results demonstrate that ASFV-like NCLDVs is extremely resilient and can maintain viability in various feed matrices during long-term storage and suggest that extending storage time alone may not be an effective mitigation practice against African swine fever virus.

Influence of extruded soybean meal with varying fat and oleic acid content on nitrogen-corrected apparent metabolizable energy in broilers

High oleic (HO) soybeans may serve as a value-added feed ingredient; providing amino acids and estimating their dietary energy value for broilers is essential. In this study, we determined the apparent metabolizable energy (AME), AME corrected for zero nitrogen retention (AMEn), digestibility, and nitrogen (N) retention of HO full-fat (HO-FF) soybean as compared to solvent-extracted soybean meal (SE-SBM), normal oleic full-fat (NO-FF) and extruded expeller (NO-EE) soybean. A total of 240 Ross-708 male broilers were selected, with eight replicates per treatment and six chicks per cage. The AME and AMEn were estimated using the difference method with a 30% inclusion of test ingredients using a corn-soy reference diet with partial and total excreta collection. The index method with partial excreta collection used titanium dioxide as an inert marker. The same starter diet was provided for all birds for 14 days, followed by the reference and assay diets for the next six adaptation days. Total excreta was collected twice a day for three days. The AME and AMEn values determined for the HO-FF and NO-FF were higher (P < 0.001) than the NO-EE and SE-SBM. The AME of SE-SBM and NO-EE were similar with both methods, but the AMEn of SE-SBM was lower than the NO-EE only with the partial collection method. The agreement between AME and AMEn values determined by partial and total excreta collection analysis was 98%. Data from the total excreta collection method yielded higher AME and AMEn values (P < 0.001) than those from the partial collection method. In summary, HO-FF and NO-FF soybean meals had similar AME and AMEn values. The HO-FF soybean had 39 and 24% higher AME and AMEn than SE-SBM. Hence, high oleic full-fat soybean meal could serve as a valuable alternative feed ingredient to conventional SE-SBM meals in broiler diets, providing additional energy while providing amino acids and more oleic acid to enrich poultry meat products.

Standardized ileal digestibility of amino acids in soybean meal fed to non-pregnant and pregnant sows

BackgroundTwo studies were designed to determine standard ileal crude protein (CP) and amino acid (AA) digestibility of soybean meal (SBM) from different origins fed to non-pregnant and pregnant sows. Seven solvent-extracted SBMs from soybeans produced in the USA, Brazil, and China were selected. In Exp. 1, eight different diets were created: a nitrogen (N)-free diet and 7 experimental diets containing SBM from different origins as the only N source. Eight non-pregnant, multiparous sows were arranged in an 8 × 8 Latin square design (8 periods and 8 diets). In Exp. 2, the diet formula was the same as in Exp. 1. Eight gestating sows (parity 3) were assigned to 4 different diets in a replicated 4 × 3 Youden square design (three periods and four diets) in mid-gestation and again in late-gestation stages.ResultsWhen fed to non-pregnant and late-gestating sows, the standardized ileal digestibility (SID) of CP and most AAs from different SBM were not significantly different (P > 0.05). When fed to mid-gestating sows, the SID values for Arg, His, Lys, Phe, Cys, Gly, Ser, and Tyr in SBM 1 were lower than in SBM 4 and 5 (P < 0.05), whereas SID for Leu from SBM 5 was higher than in SBM 1 and 4 (P < 0.05). SID values for Ile, Ala, and Asp from SBM 4 were lower than in SBM 1 and 5 (P < 0.05). Sows had significantly greater SID values for Lys, Ala, and Asp during mid-gestation when compared with late-gestation stages (P < 0.05). Mid-gestating sows had greater SID value for Val and lower SID value for Tyr when compared with non-pregnant and late-gestating sows (P < 0.01), whereas non-pregnant sows had significantly greater SID value for Met when compared with gestating sows (P < 0.01).ConclusionsWhen fed to mid-gestating sows, the SID values for most AAs varied among SBM samples. The SID values for Lys, Met, Val, Ala, Asp, and Tyr in SBM were affected by sow gestation stages. Our findings provide a cornerstone for accurate SBM use in sow diets.

Standardized ileal amino acid digestibility of high-oleic full-fat soybean meal in broilers

High-oleic (HO) soybean may serve as a value-added feed ingredient to enrich poultry meat due to its fatty acid content. However, the amino acid (AA) nutrient digestibility of soybean meal (SBM) made from these soybeans has yet to be determined. The objective of this study was to determine apparent ileal AA digestibility (AID) and standardized ileal AA digestibility (SID) of high-oleic full-fat (HO-FF) SBM compared to normal oleic full-fat (NO-FF), normal oleic extruded expeller (NO-EE), and solvent-extracted SBM (SE-SBM) in broilers. A nitrogen-free basal diet (NFD) was fed to 1 treatment group with 10 chicks/cage to determine basal endogenous losses (BEL). Titanium dioxide was used as an inert marker. The test diets contained 57.5% of the basal NFD and 42.5% of 1 of the 4 soybean sources. A total of 272 Ross-708 male broilers were placed in 40 battery cages with 5 treatments and 8 replicates per treatment. A common starter diet was provided to all the chickens for 14 d. Experimental diets were provided as a mash for 9 d before sample collection. Chickens were euthanized with CO2 on d 23, and contents of the distal ileum were collected, frozen, and freeze-dried. The BEL were similar to the values found in the literature. At d 23, broilers fed the SE-SBM had the highest body weight gain and best FCR compared to chickens fed the HO-FF and NO-FF treatments (P < 0.001). Broilers fed the SE-SBM and NO-EE experimental diets had (P < 0.001) higher apparent ileal AA digestibility and AA SID than broilers fed the HO-FF and NO-FF treatments. In conclusion, the SID of AA from HO-FF is similar to the digestibilities of other full-fat soybeans found in the literature and is lower than that of NO-EE and SE-SBM.

Counteracting Roles of Lipidic Aldehydes and Phenolic Antioxidants on Soy Protein Oxidation Defined by a Chemometric Survey of Solvent and Mechanically Extracted Soybean Meals.

Soybean meal (SBM) is a premier source of protein for feeding food-producing animals. However, its nutritional value can be compromised by protein oxidation. In this study, a total of 54 sources of solvent extracted SBM (SSBM) and eight sources of mechanically extracted SBM (MSBM), collected from different commercial producers and geographic locations in the United States during the years 2020 and 2021, were examined by chemometric analysis to determine the extent of protein oxidation and its correlation with soybean oil extraction methods and non-protein components. The results showed substantial differences between SSBM and MSBM in the proximate analysis composition, protein carbonyl content, lipidic aldehydes, and antioxidants, as well as subtle differences between 2020 SSBM and 2021 SSBM samples in protein oxidation and moisture content. Correlation analysis further showed positive correlations between protein carbonyl content and multiple lipid parameters, including the ether extract, p-anisidine value, individual aldehydes, and total aldehydes. Among the antioxidants in SBM, negative correlations with protein carbonyl content were observed for total phenolic content and isoflavone glycoside concentrations, but not for Trolox equivalent antioxidant capacity (TEAC), α-tocopherol, and γ-tocopherol. Overall, soybean oil extraction methods, together with other factors such as enzyme treatment and environmental conditions, can significantly affect the proximate analysis composition, the protein and lipid oxidation status, and the antioxidant profile of SBM. Lipidic aldehydes and phenolic antioxidants play counteracting roles in the oxidation of soy protein. The range of protein carbonyl content measured in this study could serve as a reference to evaluate the protein quality of SBM from various sources used in animal feed.

Isolation of porcine reproductive and respiratory syndrome virus from feed ingredients and complete feed, with subsequent RT-qPCR analysis.

We used virus isolation (VI) to determine tissue culture infectivity and reverse-transcription quantitative PCR (RT-qPCR) to determine the stability of porcine reproductive and respiratory syndrome virus 2 (PRRSV) strain P129 in solvent-extracted soybean meal (SBM), dried distillers grains with solubles (DDGS), complete swine feed (FEED), or medium (DMEM) at 4°C, 23°C, or 37°C for up to 3 d. Samples of each treatment were taken at regular intervals and processed. Supernatant was titrated and used to inoculate confluent MARC-145 cells to determine infectivity. RNA was extracted from each supernatant sample and tested by RT-qPCR to determine any change in detectable virus RNA across matrix type, temperature, and time. An interaction (p = 0.028) was observed for matrix × temperature × hour for live virus detected by VI. At 4°C, the concentration of infectious virus was greatest in DMEM, intermediate in SBM, and lowest in DDGS and FEED. DMEM also had the greatest concentration of infectious PRRSV at 23°C over time; a higher infectious virus concentration was maintained in SBM for longer than in DDGS or FEED. At 37°C, a greater concentration of infectious virus was sustained in DMEM than in the feedstuffs, with concentrations decreasing until 48 h post-inoculation. Only matrix type influenced the quantity of viral RNA detected by RT-qPCR (p = 0.032). More viral RNA was detected in the virus control than in DDGS; SBM and FEED were intermediate. By VI, we found that infectious virus could be harbored in SBM, DDGS, and FEED for a short time.

Nutritional evaluation of soybean meals varying in particle size

The objective of this study was to evaluate the effect of varying soybean meal (SBM) particle sizes on nutritional value of the SBM. Seven samples of dehulled solvent-extracted SBM from the same batch were ground to varying mean particles of <386, 466, 809, 1,174, 1,577, 2,026, and 2,321 μm. Two precision-fed rooster assays (crop intubation with 25 g of SBM followed by 48 h total excreta collection) were performed to determine TMEn and standardized amino acid (AA) digestibility. There were no significant differences for TMEn among SBM samples, and there was also no consistent significant effect of particle size on standardized AA digestibility. In addition to the 2 precision-fed rooster assays, a 21 d broiler chick trial was conducted using corn-SBM based diets using 4 diets that differed only in the mean particle size of SBM (466, 809, 1,174, or 1,577 μm), being fed from 2 to 23 d of age. Chicks fed diets containing 809 or 1,174 μm SBM had increased (P < 0.05) weight gain compared with chicks fed the diet containing 466 μm SBM, and chicks fed diets containing 1,174 or 1,577 μm SBM had increased (P < 0.05) feed efficiency compared with chicks fed the diet containing 466 μm SBM. The diet containing 466 μm SBM yielded the highest (P < 0.05) AMEn and total tract P retention. Ileal P digestibility and standardized AA digestibilities did not differ among treatments. Relative gizzard weight (percent of body weight) was increased (P < 0.05) by the 2 largest SBM particle sizes. The results from these 3 experiments showed that increasing SBM particle size may be beneficial to broiler growth performance and may increase gizzard size but had no consistent significant effect on ME, AA digestibility, or P digestibility/retention.

Amino acid digestibility and metabolizable energy of soybean meal of different origins in cecectomized laying hens

This study investigated the variation in amino acid (AA) digestibility and MEn of 18 samples of solvent-extracted soybean meal (SBM; 6 European, 7 Brazilian, 2 Argentinian, 2 North American, 1 Indian) in cecectomized laying hens. The experimental diets contained either 300 g/kg of cornstarch or one of the SBM samples. Pelleted diets were fed to 10 hens in two 5×10 row-column designs so that 5 replicates were obtained from each diet during 5 periods. A regression approach and the difference method were used to determine AA digestibility and MEn, respectively. The variation in the digestibility of SBM differed among AA with ranges in digestibility of 6 to 12%-units for most AA. Among the first-limiting AA, the digestibility was 87 to 93%, 63 to 86%, 85 to 92%, 79 to 89%, and 84 to 95% for Met, Cys, Lys, Thr, and Val, respectively. The range of MEn for the SBM samples was 7.5 to 10.5 MJ/kg DM. Indicators of SBM quality (including trypsin inhibitor activity, KOH solubility, urease activity, and in vitro N solubility) and analyzed SBM constituents were significantly correlated (P ≤ 0.05) with AA digestibility or MEn only in a few cases. No differences were observed in AA digestibility and MEn between countries of origins, except low digestibility of some AA and MEn for the 2 Argentinian SBM samples. These results suggest that the precision of feed formulation benefits from considering the variations in AA digestibility and MEn. Often used indicators for SBM quality and analyzed constituents were not suitable to explain variations in AA digestibility and MEn, suggesting that AA digestibility and MEn are determined by other factors.

Using caloric efficiency to estimate the net energy value of expelled, extruded soybean meal relative to dehulled, solvent-extracted soybean meal and its effects on growth performance of nursery pigs.

This study aimed to estimate the net energy (NE) value of expelled, extruded soybean meal (MSBM) relative to dehulled, solvent-extracted soybean meal (SSBM) and determine its effects on growth performance of late nursery pigs. A total of 297 pigs (DNA 241 × 600) were weaned (BW 5.10 kg) and placed into 60 pens (2 rooms of 30 pens) with 5 pigs per pen balanced by gender and weaning weight. Pigs were fed a common diet for 21 d. Then, pens of pigs (BW 9.3 kg) were randomly assigned to one of five treatments to provide 12 replications per treatment. Treatments consisted of increasing amounts of MSBM replacing SSBM in the diet (0%, 25%, 50%, 75%, and 100%). All diets were fed for 28 d and were formulated to 1.30% standardized ileal digestible lysine and met or exceeded requirements for amino acids, calcium, and phosphorus. The SSBM diet was formulated to 2,421 kcal/kg and NE was not balanced between diets. Analyzed values for CP, EE, CF, and total lysine for the SSBM were 47.28%, 0.47%, 3.80%, and 3.00%, whereas the MSBM contained 47.41%, 6.88%, 5.32%, and 2.99%, respectively. The MSBM had increased values for KOH solubility and trypsin inhibitor (83.62% and 7,026 TIU/g) compared to the SSBM (73.05% and 3,011 TIU/g), whereas urease activity was similar between the two (0.03 and 0.02 Δ pH, respectively). Data were analyzed using Proc GLIMMIX (SAS 9.4; Cary, NC) with pen as the experimental unit and room as the blocking factor. There was no evidence of differences in ADG and ADFI in pigs fed diets with increasing concentrations of MSBM. Pigs fed diets with increasing concentrations of MSBM had improved (linear, P < 0.001) G:F and caloric efficiency on an NE basis. Using caloric efficiency to estimate NE of the MSBM relative to SSBM, MSBM was estimated to have a value of 2,566 kcal/kg. In conclusion, MSBM contains approximately 123% of the energy of SSBM, which improved feed efficiency when fed to nursery pigs.

Effects of partial replacement of solvent-extracted soybean meal by amino resin-treated soybean meal in the concentrate supplement of high producing grazing dairy cows

We aimed to determine the effects of partially replacing conventional solvent-extracted soybean meal by amino resin-treated soybean meal in the concentrate supplement of lactating dairy cows grazing Guinea grass (Panicum maximum cv. Mombaça) on dry matter intake, milk production and composition, feed and nitrogen use efficiency, and nutrient digestibility. Eighteen Holstein x Gyr crossbred multiparous cows (79 ± 38 days in milk and 37.4 ± 4.69 kg/d of milk yield) were used in a replicated 3 × 3 Latin square design study with three experimental periods of 21 d The solvent extract soybean meal of the control diet (CON) was replaced by 1.0 (TSBM1.0) and 1.5 (TSBM1.5) kg as-fed basis of amino resin-treated soybean meal (29 and 43% as dry-matter basis, respectively) in the treatment diets. Dry matter intake, milk casein, protein, and fat contents, feed efficiency, nitrogen use efficiency, and nutrient digestibility did not differ among treatments (P > 0.05). The metabolizable protein was not increased by the treatments; however, cows fed with TSBM1.5 tended to produce 1.2 kg/d more milk (P = 0.09) and 64.3 g/d more lactose (P = 0.06) than CON cows. Although the supply of metabolizable protein was not affected by treatments, increasing rumen undegradable protein supply by 43% by replacing conventional solvent-extracted soybean meal with amino resin-treated soybean meal tended to increase milk production of high producing grazing cows.

Growth performance of male broilers fed ExPress® soybean meal and high-shear dry extruded corn

Soybean meal ( SBM ), a co-product of soybean oil obtained via solvent or mechanical extrusion, is the key protein source in broiler diet formulations. Mechanical extrusion, or high-temperature processing with high-shear dry extrusion, has demonstrated 1 to 2% increased amino acid digestibility in broilers versus solvent-extracted SBM through reduction of antinutritional factors ( ANF ). High-shear dry extrusion followed by mechanical pressing using a screw press (extruding-pressing) produces soy oil and partially de-oiled soymeal (ExPress). The extruding-pressing process has been growing in popularity due to improved cost and material efficiency by eliminating the need for solvents or steam dryers in the production of animal feed ingredients. Corn extrusion is likewise linked with increased nutritive value through improved palatability and approximately 20% increased starch gelatinization, resulting in superior growth performance and in one reported case, reduced mortality in broilers. In the present study, high-shear dry extruded corn and ExPress SBM were formulated in combination with conventional corn and solvent-extracted SBM to determine impact on growth performance in male broilers. Extruded corn increased feed intake and ExPress SBM increased weight gain. The diet containing dry extruded corn and ExPress SBM outperformed all other diet combinations, including unprocessed, ground corn and conventional, solvent-extracted SBM variations. The diet containing unprocessed, ground corn and ExPress SBM had the most efficient overall feed conversion ratio ( FCR ). The combination of lower feed intake observed with the ground corn ingredient and the improved protein digestibility reflected by superior growth in ExPress SBM made this the ideal formulation, out of the 4 diets examined, in terms of feed efficiency.

Diet supplementation with canola meal improves milk production, reduces enteric methane emissions, and shifts nitrogen excretion from urine to feces in dairy cows

The objective of this study was to examine the effect of isonitrogenous substitution of solvent-extracted soybean meal (SBM) with solvent-extracted canola meal (CM) on enteric CH4 production, ruminal fermentation characteristics (including protozoa), digestion (in situ and apparent total-tract digestibility), N excretion, and milk production of dairy cows. For this purpose, 16 lactating Holstein cows, of which 12 were ruminally cannulated, were used in a replicated 4 × 4 Latin square (35-d periods; 14-d adaptation). The cows averaged (mean ± SD) 116 ± 23 d in milk, 692 ± 60 kg of body weight, and 47.5 ± 4.9 kg/d of milk production. The experimental treatments were control diet (no CM; 0%CM) and diets supplemented [dry matter (DM) basis] with 7.9% CM (8%CM), 15.8% CM (16%CM), or 23.7% CM (24%CM) on a DM basis. The forage:concentrate ratio was 52:48 (DM basis) and was similar among the experimental diets. Canola meal was included in the diet at the expense of SBM and soybean hulls, whereas the percentages of the other diet ingredients were the same. Intake of DM increased linearly, whereas apparent total-tract digestibility of DM, crude protein, neutral detergent fiber, and gross energy (GE) declined linearly as CM inclusion in the diet increased. Total volatile fatty acids concentration and butyrate molar proportion decreased linearly, whereas molar proportion of propionate increased linearly, and that of acetate was unaffected by CM inclusion in the diet. Ruminal ammonia concentration was not affected by inclusion of CM in the diet. Energy-corrected milk (ECM) yield increased linearly (up to 2.2 kg/d) with increasing CM percentage in the diet, whereas milk production efficiency averaged 1.63 kg of ECM/kg of DM intake and was unaffected by CM inclusion in the diet. Daily CH4 production decreased linearly with increasing CM percentage in the diet (489, 475, 463, and 461 g/d for 0%CM, 8%CM, 16%CM and 24%CM diets, respectively). As a consequence, CH4 emission intensity (g of CH4/kg of ECM) also declined linearly by up to 10% as the amount of CM increased in the diet. Methane production also decreased linearly when expressed relative to GE intake (5.7, 5.2, 5.1, and 4.9% for 0%CM, 8%CM, 16%CM and 24%CM diet, respectively). Quantity of manure N excretion was not affected by replacing SBM with CM; however, N excretion shifted from urine to feces as dietary percentage of CM increased, suggesting reduced potential for N volatilization. Results from this study show that replacing SBM with CM as a protein source in dairy cow diets reduced enteric CH4 emissions (g/d, % of GE intake, and adjusted for milk production) and increased milk production. The study indicates that CM can successfully, partially or fully, replace SBM in lactating dairy cow diets, with positive effects on animal productivity and the environment (i.e., less enteric CH4 emission and urinary N excreted). We conclude that compared with SBM, inclusion of CM meal in dairy cow diets can play a key role in reducing the environmental footprint of milk production.

31 Using Caloric Efficiency to Estimate the Energy Value of Expelled, Extruded Soybean Meal Relative to Dehulled, Solvent-extracted Soybean Meal and Its Effects on Growth Performance of Nursery Pigs

Abstract This study aimed to estimate the net energy value of expelled, extruded soybean meal (MSBM) relative to dehulled, solvent-extracted soybean meal (SBM) and determine its effects on growth performance of late nursery pigs. Analyzed values for CP, EE, CF, and lysine for the SBM were 47.28%, 0.47%, 3.80%, and 3.00% while the MSBM contained 47.41%, 6.88%, 5.32%, 2.99% respectively. A total of 297 pigs (DNA 200 x 400) were placed into 60 pens (2 rooms of 30 pens) with 5 pigs per pen balanced by gender and weaning weight. Pigs were fed common diets for 21 days. Then, pens of pigs (BW 9.3 kg) were randomly assigned to one of five treatments to provide 12 replications per treatment. Treatments consisted of increasing amounts of MSBM replacing SBM in the diet (0, 25, 50, 75, 100%). All diets were fed for 28 days and were formulated to 1.30% standardized ileal digestible lysine and met or exceeded NRC (2012) recommendations for lysine:amino acids, calcium, and phosphorus. The SBM diet was formulated to 2421 kcal/kg and net energy (NE) was not balanced between diets. Data were analyzed using Proc GLIMMIX (SAS 9.4; Cary, NC) with pen as the experimental unit and room as the blocking factor. There was no evidence of differences in ADG and ADFI in pigs fed diets with increasing concentrations of MSBM. Pigs fed diets with increasing concentrations of MSBM had improved (linear, P &amp;lt; 0.001) G:F and caloric efficiency on an NE basis. In conclusion, using caloric efficiency to estimate NE of the MSBM relative to SBM, MSBM was estimated to have a value of 123% of SBM NE or 2566 kcal/kg. This increase in NE resulted in improved feed efficiency of nursery pigs.

In sacco evaluation of ruminal degradability of isoflavones from full-fat soybean and extracted soybean meal-A pilot study.

The aim of the study was to determine the ruminal degradability of dry matter (DM), daidzein, genistein, glycitein and total isoflavones in ground full-fat soybean (GFFS) and solvent-extracted soybean meal (SSBM) using the in sacco method. The experiment was carried out in three replications on ruminally cannulated sheep that were fed twice a day with a diet consisted of hay and supplemental mixture (6:4, DM basis). The nylon bags with 2g feed samples ground to 2mm were incubated in the rumen for 0, 2, 4, 8, 16 and 24h. The effective degradability (ED) of DM, daidzein, genistein, glycitein and total isoflavones was calculated at outflow rate of 0.06h. The ED of DM in GFFS was 77.8% and was higher than in SSBM being 71.8% (p<0.001). The ED of daidzein (96.8%) and genistein (93.6%) was higher for SSBM compared with GFFS (93.9% and 92.8%, p<0.001 and p=0.003, respectively) while ED of glycitein was lower for SSBM than for GFFS (75.5 and 81.7%, respectively, p<0.001). All isoflavones in the incubations were extensively degraded in the rumen, and regardless of dietary source, they were almost completely degraded after 16h of incubation. Further, the disappearance patterns, that is the functions describing the time courses of the analyte disappearance, were assessed. The disappearance patterns of daidzein, genistein, glycitein and total isoflavones were similar and showed greater disappearance of mentioned isoflavones from SSBM compared to GFFS (p<0.001 for daidzein, genistein and total isoflavones and p=0.002 for glycitein). The study provides knowledge on the effect of processing on degradability of isoflavones in rumen that can be used to clarify the interrelationship between isoflavones and rumen microbiota.

Lactational performance, enteric gas emissions, and plasma amino acid profile of dairy cows fed diets with soybean or canola meals included on an equal protein basis

This study investigated the effects of feeding solvent-extracted canola meal (CM), extruded soybean meal (ESBM), or solvent-extracted soybean meal (SSBM) on an equivalent crude protein basis on performance, plasma AA profiles, enteric gas emissions, milk fatty acids, and nutrient digestibility in lactating dairy cows. Fifteen Holstein cows (95 ± 20 d in milk) were used in a replicated 3 × 3 Latin square design experiment with 3 periods of 28 d each. Treatments were 3 diets containing 17.1% CM, 14.2% ESBM, or 13.6% SSBM (dry matter basis). Vegetable oil was added (canola oil for CM or soybean oil for SSBM) to equalize the ether extract concentration of the diets. Rumen-protected Met was supplemented targeting digestible Met supply of 2.2% of metabolizable protein in all diets. Canola meal increased dry matter intake (DMI) by 5.9 and 8.9% in comparison with ESBM and SSBM, respectively. Milk urea nitrogen was lowest in CM, followed by SSBM, and was highest for ESBM. No differences were observed in feed efficiency, energy-corrected milk yield, and milk composition or component yields among treatments. Cows fed CM emitted less enteric CH4 per kg of DMI compared with both ESBM and SSBM, but CH4 emission intensity (CH4 per kg of energy-corrected milk) was similar among treatments. In summary, replacement of ESBM or SSBM with CM, on an equal crude protein basis, in the diet of lactating dairy cows enhanced DMI, but yields of energy-corrected milk and milk components and feed efficiency were similar among treatments.

Use of fat-coated or heat-treated soybean meal for partial replacement of solvent-extracted soybean meal in the diets of early lactation dairy cows

Context Soybean meal (SBM) is the most important protein source used to feed dairy cows, and methods have been developed to increase its nutritional value by protecting the proteins against rumen degradation. Protection of solvent-extracted SBM with saturated fats can achieve this, but effects on lactation performance and nutrient digestibility have not been investigated. Aims We evaluated effects on performance, nutrient digestibility and blood metabolites of high-yielding dairy cows when dietary solvent-extracted SBM was partially replaced with two fat-coated SBM products containing palmitic acid or a palmitic–stearic acid mix, or with standard heat-treated SBM. Methods Forty lactating Holstein cows were used in a randomised complete block design experiment with two phases each of 26 days (20 days of diet adaptation and 6 days of data collection). Experimental diets comprised (per kg DM) 271 g maize silage, 138 g other forages and 590 g concentrate, with 6.74 MJ net energy for lactation, 157 g crude protein and 366 g neutral detergent fibre. The control diet contained solvent-extracted SBM at 73.1 g/kg, which was partially replaced in the three test diets: two with fat-coated SBM (400 g fat/kg, enriched with palmitic acid or 50:50 palmitic:stearic acids), and one with heat-treated SBM. Diets were offered ad libitum during the experiment, and cows were milked three times daily. Key results Solvent-extracted SBM had significantly lower rumen undegraded protein concentration than the other three products. Dry matter intake was not affected by dietary treatment (27.7 ± 0.531 kg/day), nor was milk yield (47.8–50.3 kg/day), but milk fat yield tended to be greater (P &lt; 0.10) with diets containing fat-coated than solvent-extracted SBM. The diet with palmitic acid coated SBM showed higher apparent total tract digestibilities of ether extract, dry matter, organic matter, crude protein and neutral detergent fibre than palmitic:stearic acid coated or solvent-extracted SBM treatments. Heat-treated SBM diet likewise showed higher digestibilities of ether extract, dry matter, organic matter, and acid and neutral detergent fibres than the solvent-extracted SBM diet. Concentrations of urea nitrogen in plasma and milk were not affected by treatment. Conclusions Feeding heat-treated or fat-coated SBM did not increase milk production of high-yielding cows; however, use of fat-coated SBM increased milk fat yield. Both palmitic acid coating and heat treatment improved total tract digestibility. Implications Feeding fat-coated SBM to dairy cows can protect SBM in the rumen and increase milk fat yield.

Estimating the Net Energy Value of Expelled, Extruded Soybean Meal When Fed to Nursery Pigs

Solvent-extracted soybean meal (SSBM) is typically used as the primary protein source in swine diets in the United States. Mechanically extracted or expelled soybean meal (MSBM) typically has increased concentrations of fat and decreased concentrations of essential AA compared to SSBM, but MSBM has been demonstrated to have improved AA digestibility. This study aimed to estimate the NE value of MSBM relative to SSBM and to determine its effects on growth performance of late nursery pigs. A total of 297 pigs (241 × 600, DNA) were weaned (BW 11.2 lb) and placed into 60 pens (2 rooms of 30 pens) with 5 pigs per pen balanced by gender and weaning weight. Pigs were fed a common diet for 21 days. Then, pens of pigs (BW 20.7 lb) were randomly assigned to one of five treatments to provide 12 replications per treatment. Treatments consisted of increasing amounts of MSBM replacing SSBM in the diet (0, 25, 50, 75, and 100%). All diets were fed for 28 days and were formulated to 1.30% standardized ileal digestible lysine, and met or exceeded NRC2 recommendations for Lys:AA, Ca, and P. The SSBM has an NRC2 NE value of 946 kcal/lb and the 0% MSBM diet was formulated to 1091 kcal/lb and NE was not balanced between diets. Analyzed values for CP, ether extract, crude fiber, and total Lys for the SSBM were 47.28%, 0.47%, 3.80%, and 3.00%, respectively, while the MSBM contained 47.41%, 6.88%, 5.32%, and 2.99% respectively. The MSBM had increased values for KOH solubility and trypsin inhibitor (83.62 and 7026 TIU/g, respectively) compared to the SSBM (73.05 and 3011 TIU/g, respectively) while urease activity was similar between the two (0.03 vs. 0.02 Δ pH, respectively). Data were analyzed using PROC GLIMMIX (SAS v. 9.4) with pen as the experimental unit and room as the blocking factor. There was no evidence of differences in ADG and ADFI in pigs fed diets with increasing concentrations of MSBM. Pigs fed diets with increasing concentrations of MSBM had improved (linear, P < 0.001) F/G and caloric efficiency on an NE basis. In conclusion, using caloric efficiency to estimate NE of the MSBM relative to SSBM, MSBM was estimated to have a value of 123% of SSBM NE or 1,164 kcal/lb. This increase in NE resulted in improved feed efficiency of nursery pigs.

Milk production responses, rumen fermentation, and blood metabolites of dairy cows fed increasing concentrations of forage rape (Brassica napus ssp. Biennis)

The aim of this study was to determine animal performance, rumen fermentation, and health-related blood metabolites of dairy cows in mid lactation fed with increasing levels (30 and 45%) of forage rape (FR) in the diet. Twelve pregnant multiparous lactating Holstein-Friesian dairy cows were randomly allocated to 1 of 3 dietary treatments in a replicated 3 × 3 Latin square design. The experiment was divided into three 21-d periods. For the control diet, 13.0 kg (dry matter, DM) of grass silage, 3.0 kg DM of commercial concentrate, 2.7 kg of DM cold-pressed extracted canola meal, and 0.45 kg DM of solvent-extracted soybean meal were offered daily. For the other two treatments, 30 and 45% of the DM from silage, canola meal, and commercial concentrate were replaced in equal proportions with FR. Data were analyzed individually using linear and quadratic orthogonal polynomials. Ingestive behavior was altered by the inclusion of FR. We observed a linear increase in eating time at the expense of rumination time. Nevertheless, total DM intake was not affected by dietary treatments, averaging 19.5 ± 0.24 kg of DM/d. Milk yield increased linearly with increasing concentration of FR in the diet. Thus, feed efficiency of cows (kg of milk/kg of DM intake) increased linearly with the percentage of FR in the diet. Inclusion of FR in the diet had no effect on milk composition or milk sensory characteristics. Mean rumen pH of cows decreased linearly from the control to the 45% FR diet; however, dietary treatments had no effect on the daily amount of time that rumen pH was below 5.8 (252 ± 71.4), indicating no risk of subacute ruminal acidosis. Concentrations of total volatile fatty acids in the rumen and molar proportions of acetate and butyrate were increased with FR inclusion, whereas the proportion of propionate was linearly reduced. Excretion of uric acid and total purine derivatives tended to be greater for cows fed FR, which resulted in a trend toward a linear increase in estimated microbial N flow. However, N use efficiency was not affected by FR inclusion. Although differences for some hematological measures (increased white blood cell and neutrophils counts) and a quadratic response for glutamate dehydrogenase for cows fed FR in the diet (decreased with inclusion of 30% and increased with 45% in the diet) were observed, all values were within appropriate ranges for dairy cows. These results indicated that including FR to dairy cow diets, up to 45% of diet DM, improved milk production due to changes in volatile fatty acids and predicted microbial N flow and had no negative effects on dairy cow health or sensory characteristics of milk.