Solvent Compatibility Research Articles

Efficiency of an alkaline, thermostable, detergent compatible, and organic solvent tolerant lipase with hydrolytic potential in biotreatment of wastewater

Discharging the tannery wastewater into the environment is a serious challenge worldwide due to the release of severe recalcitrant pollutants such as oil compounds and organic materials. The biological treatment through enzymatic hydrolysis is a cheap and eco-friendly method for eliminating fatty substances from wastewater. In this context, lipases can be utilized for bio-treatment of wastewater in multifaceted industrial applications. To overcome the limitations in removing pollutants in the effluent, we aimed to identify a novel robust stable lipase (PersiLipase1) from metagenomic data of tannery wastewater for effective bio-degradation of the oily wastewater pollution. The lipase displayed remarkable thermostability and maintained over 81 % of its activity at 60 °C.After prolonged incubation for 35 days at 60°C, the PersiLipase1 still maintained 53.9 % of its activity. The enzyme also retained over 67 % of its activity in a wide range of pH (4.0 to 9.0). In addition, PersiLipase1 demonstrated considerable tolerance toward metal ions and organic solvents (e.g., retaining >70% activity after the addition of 100 mM of chemicals). Hydrolysis of olive oil and sheep fat by this enzyme showed 100 % efficiency. Furthermore, the PersiLipase1 proved to be efficient for biotreatment of oil and grease from tannery wastewater with the hydrolysis efficiency of 90.76 % ± 0.88. These results demonstrated that the metagenome-derived PersiLipase1 from tannery wastewater has a promising potential for the biodegradation and management of oily wastewater pollution.

Enhanced optical trapping of ZrO2@TiO2 photonic force probe with broadened solvent compatibility.

Optical trapping and manipulating nanoparticles are essential tools for interrogating biomedicine at the limits of space and time. Typically, silica or polystyrene microspheres are used as photonic force probes. However, adapting those probes to organic solvents is an ongoing challenge due to the limited solvent compatibility and low refractive index mismatch. Here we report on the optical force enhancement and solvent compatibility that utilizes ZrO2@TiO2 core-shell nanoparticles. We experimentally demonstrate that the 450-nm-diameter ZrO2@TiO2 core-shell nanoparticles achieve the lateral and axial trap stiffness up to 0.45 pN µm-1 mW-1 and 0.43 pN µm-1 mW-1 in water, showing more than fivefold and ninefold improvement on the ordinary SiO2 particle of the same size. In addition, ZrO2@TiO2 core-shell nanoparticles can realize stable three-dimensional trapping in both polyethylene glycol and glucose solutions. This optical trapping enhancement property, coupled with solvent compatibility, expands the range of feasible optical trapping experiments and will pave the way toward more advanced biological applications.

Spontaneously Healing Buried Interfaces in n–i–p Halide Perovskite Photovoltaics

Accumulated halide defects on the buried interfaces of halide perovskite layers have exacerbated undesirable nonradiative recombination in the n–i–p perovskite photovoltaics, but are challenging to be passivated—the commonly used passivation molecules at buried interfaces of perovskite layers would be inevitably eroded in the solution processes of perovskite deposition. Regarding the solvent incompatibility, herein, the ZnO–EA/SnO2–Cl electron transfer layers (ETLs) terminated with functional sites (i.e., ethanolamine (EA) ligands on ZnO and Cl− ions on SnO2) to spontaneously heal the buried interfaces of perovskite layers are customized. The specialties of ZnO–EA/SnO2–Cl for defect passivation are revealed: 1) formation of ZnO–EA–Pb2+ coherent interlayers at the EA‐terminated ZnO‐perovskite interfaces effectively offsets the I vacancy defects of perovskites; and 2) spontaneous halide exchange between Cl−‐terminated SnO2 and unstable I−‐terminated perovskites enables the formation of FA2Sn(ICl)6‐like coherent interlayers. Thus, the customized termination of ETLs’ surface reduces the halide‐defect‐triggered nonradiative recombination at the buried surfaces of perovskite, enabling the fabricated n–i–p planar modules (6 × 6 cm2) with power conversion efficiencies approaching 18% and elevated stability. These findings provide desirable guidelines for interfacial carrier transport between perovskites and ETLs.

A solvent-compatible filter-transfer method of semi-transparent carbon-nanotube electrodes stacked with silver nanowires

ABSTRACT Low-density films of single-walled carbon nanotubes (SWNTs) can be used as a semi-transparent top electrode for all-solution-processed film devices; however, their semiconductor characteristics vary depending on the experimental factors in their dispersion into solvents, and the sublayers are damaged as a result of solvent incompatibility. In this study, we report a solvent-compatible filter-transfer method for SWNT films stacked with silver nanowires (AgNWs), and evaluate the semiconductor characteristics through the p/n heterojunction with a Si wafer (SWNT/Si). AgNWs and SWNTs were successively filtered through their aqueous dispersion solutions using a membrane filter. The stacked semi-transparent films (AgNW/SWNT films with controlled densities) were successfully transferred onto glass plates and Si wafers. The transmittance at 550 nm revealed a window between 60% and 80% with a narrow sheet resistance range between 11 and 23 Ω □−1. The power conversion efficiency (PCE) of SWNT/Si was improved to 11.2% in a junction area of 0.031 cm2 through the use of spin-coated Nafion resins; however, the accumulated resistance of SWNTs drastically reduced the PCE to 2% as the area increased to ≥0.5 cm2. AgNWs maintained the PCE within a range of 10.7% to 8.6% for an area ranging from 0.031 cm2 to 1.13 cm2. All of the photovoltaic parameters were dependent on the junction areas, suggesting that AgNWs function as an effective current-collector layer on the semiconductor layer of SWNTs without direct contact of AgNWs with the Si surface. In addition, we report a solvent-compatible experiment for transferring AgNW/SWNT films onto a solvent-sensitive perovskite material (CH3NH3PbI3).

Resolving Solvent Incompatibility in Two-Dimensional Liquid Chromatography with In-Line Mixing Modulation.

Two-dimensional liquid chromatography (2D-LC) is a powerful technique used to characterize complex samples such as synthetic polymers, biomacromolecules, and hybrid modalities (conjugates, oligonucleotides, nanoparticles, etc., which fall between traditional small molecules and large molecules). Characterizing such molecules often requires a highly orthogonal 2D-LC workflow to resolve structurally similar impurities. However, it remains a challenge to achieve truly orthogonal 2D-LC coupling due to incompatibility of the chromatographic conditions used in each dimension. In this work, we present a facile strategy of connecting an in-line mixer, in-line mixing modulation (ILMM), to realize challenging 2D-LC workflows: (1) coupling gel permeation chromatography (GPC) with reversed-phase liquid chromatography (RPLC) for hydrophobic oligomer analysis and (2) coupling ion-pair reversed-phase (IPRP) with hydrophilic interaction liquid chromatography (HILIC) for polar antisense oligonucleotide (ASO) analysis. Compared with the state-of-the-art commercially available active solvent modulation (ASM), engaging the ILMM significantly reduces the peak distortion in GPC-RPLC, allowing an at least 67% higher transfer volume from the primary to secondary dimension, and resolves the ASO sample breakthrough in selective comprehensive IPRP×HILIC. Also remarkably, ILMM demonstrated superiority in comprehensive RPLC×RPLC analysis in comparison with ASM, suggesting its potential in broader 2D-LC applications. In addition to chromatography improvement, ILMM offers several advantages over benchmark modulation approaches in regard to alleviating the need of an additional dilution flow and a simple as well as flexible system configuration, opening many opportunities to establish innovative and versatile multidimensional workflows for characterizing compounds with increasing complexity.

Profile of Intravenous Preparation Mixing in Patients at the Regional General Hospital's Intensive Care Unit Abdoel Wahab Sjahranie Samarinda

Compatibility is one of the quality characteristics of intravenous (IV) preparations that influences the effectiveness of patient therapy in the intensive care Unit (ICU). This study aimed to determine the compatibility profile of IV medication formulations given to ICU patients. This prospective observational research was undertaken on patients in the ICU of a private hospital in Samarinda. The data on the intravenous combination of drugs were compared in this study utilizing the ASHP Injectable Drug Information literature 21st edition, Injectable drug guide, and Manual Book. Injectable Drug Mixing was published in 2009 as a resource for determining the compatibility of intravenous formulations. Using certain criteria, combinations of intravenous medicines and their solvents are classed as compatible (C), incompatible (I), and unknown (U). The ICU had 25 patients, 15 of whom were male, and 10 of them were female. There is no mixing of drug compounds containing incompatible solvents. All drugs were carried out with 100% compatible solvents. The limited information related to the compatibility and stability of these intravenous preparations encourages continuous monitoring of the patient's condition and drug levels.

Acyclic and Heterocyclic Azadiene Diels-Alder Reactions Promoted by Perfluoroalcohol Solvent Hydrogen Bonding: Comprehensive Examination of Scope.

Herein, the first use of perfluoroalcohol H-bonding in accelerating acyclic azadiene inverse electron demand cycloaddition reactions is described, and its use in the promotion of heterocyclic azadiene cycloaddition reactions is generalized through examination of a complete range of azadienes. The scope of dienophiles was comprehensively explored; relative reactivity trends and solvent compatibilities were established with respect to the dienophile as well as azadiene; H-bonding solvent effects that lead to rate enhancements, yield improvements, and their impact on regioselectivity and mode of cycloaddition are defined; new viable diene/dienophile reaction partners in the cycloaddition reactions are disclosed; and key comparison rate constants are reported. The perfluoroalcohol effectiveness at accelerating an inverse electron demand Diels-Alder cycloaddition is directly correlated with its H-bond potential (pKa). Not only are the reactions of electron-rich dienophiles accelerated but those of strained and even unactivated alkenes and alkynes are improved, including representative bioorthogonal click reactions.

Effect of Solvents on a Li10GeP2S12-Based Composite Electrolyte via Solution Method for Solid-State Battery Applications.

Using a solution approach to process composite electrolytes for solid-state battery applications is a viable strategy for lowering the thickness of electrolyte layers and boosting the cell energy density. To fully utilize the super ionic conductivity of sulfides, more research about their solvent and binder compatibility is needed. Herein, the allowable solvent polarity is discovered through systematically pairing the solid electrolyte Li10GeP2S12 (LGPS) with eight types of aprotic solvents. To further consider the influence of oxygen and moisture solvation that is important to practical manufacturing scenario, we also design experiments to flow dry air and N2, or further mixed with water vapor, through these solvents to unveil their detrimental effects. Finally, a low polar solvent, dimethyl carbonate (DMC), and a previously unfavored commercial polymer, poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP), are chosen to fabricate a ∼40 μm thick LGPS-based composite electrolyte, giving 2 mS·cm-1 conductivity. It cycles between lithium/graphite composite electrodes at 0.5 mA·cm-2 for over 450 h with a capacity of 0.5 mAh·cm-2 and can withstand a 10-fold current surge.

Deterministic fabrication of 3D/2D perovskite bilayer stacks for durable and efficient solar cells.

Realizing solution-processed heterostructures is a long-enduring challenge in halide perovskites because of solvent incompatibilities that disrupt the underlying layer. By leveraging the solvent dielectric constant and Gutmann donor number, we could grow phase-pure two-dimensional (2D) halide perovskite stacks of the desired composition, thickness, and bandgap onto 3D perovskites without dissolving the underlying substrate. Characterization reveals a 3D-2D transition region of 20 nanometers mainly determined by the roughness of the bottom 3D layer. Thickness dependence of the 2D perovskite layer reveals the anticipated trends for n-i-p and p-i-n architectures, which is consistent with band alignment and carrier transport limits for 2D perovskites. We measured a photovoltaic efficiency of 24.5%, with exceptional stability of T99 (time required to preserve 99% of initial photovoltaic efficiency) of >2000 hours, implying that the 3D/2D bilayer inherits the intrinsic durability of 2D perovskite without compromising efficiency.

Effects of halogen and hydrogen halide on graphene: Halogenation and exfoliation

Halogenated graphene can be prepared by liquid phase exfoliation due to good balance between quality and cost. However, this method is severely hampered by lack of understanding of microscopic mechanisms, for example, the influence of polarity of solvent and the reaction between solvent and defect which determines exfoliating and the functionalization. Here, we investigate the effects of HX (X = F, Cl, Br, I), Br2 and I2 on the preparation of halogenated graphene using first principles. We found that solvent molecules can act as charge transfer mediators between layers to produce adhesion, which is greatly discourages separation between layers. Besides, the orientation of hydrogen will greatly affect the halogenation of graphene. This work provides abundant theoretical guidance for the preparation of many materials, including but not limited to halogenated graphene, by liquid phase exfoliation, such as the influence of the polarity of solvents and the compatibility of solvents with point defects.

Synergistic interface-pillared Fe-MOF on 2D Ti3C2TX MXene electrode coupling toward high energy density

Compared with most transition metal based electrode materials, 2D Mn+1XnTx has high conductivity and good solvent compatibility. However, its low energy density hinders its application as supercapacitior electrode for electrochemical energy storage applications. Herein, the interface-pillared of Fe-MOF (MIL-100(Fe)) onto Ti3C2Tx MXene layer, which provide rich electrochemical active sites, is achieved, and the MOF nanospheres with MXene nanosheets are subsequently converser into three dimensional multilayer porous nanostructures through the chemical bond couping. Therefore, the MOF nanospheres are anchored between the nansheets of MXene, enabling the fast ion transportation in the three dimensional nanostructures. Menanwhile, the MOF nanospheres prevent the aggregation of MXene nanosheets, and the laminar structure is maintained for more redox reaction sites within the hybrid electrodes through fast charge–discharge cycles. As a result, the MIL-100(Fe)/Ti3C2Tx MXene electrode displays superior energy density (85.53 Wh kg−1), which is much higher than the other MXene-based electrodes as far as we know. This design strategy of 2D hybrid electrodes opens up a new way for the commercial application of high perfermance supercapacitor.

Revealing the mechanism of electrochromic sticking image behavior in tungsten oxide film prepared from tungstate precursors

Ammonium tungstate precursor system are one of promising preparation routes for sol-gel tungsten oxide (WO3) film because of its low-cost preparation, good solution stability, and good solvent compatibility. Nevertheless, we notice that an incomplete ion extraction (called electrochromic sticking image) behavior of electrochromic WO3 film prepared from tungstate precursor, which weakens its electrochromic performance. In this work, some key factors such as residual precursor ammonium ions and oxygen vacancy defects are investigated to understand the electrochromic sticking image behaviors. For the amorphous WO3 film, the film components consist of WO3 and tungstate. The endogenic ammonium ions can preferentially participate in the coloring process to occupy the WO3 lattices. However, in the bleaching process, there is always part of ions remaining in the film due to electrical neutrality principle (between tungstate anions and positive ions), resulting in the electrochromic sticking image. For the crystalline WO3 film, the increasing concentration of oxygen vacancy and film density become a main factor that inhibits the extraction of Li+ ions, which is reflected by the low diffusion coefficient and high charge transfer resistance. For this case, a long-term bleaching bias is valid to eliminate the electrochromic sticking images for crystalline WO3 film. This work provides a mechanism understanding for improving the electrochromic memory of sol-gel WO3 film.

Simple Full-Spectrum Heterogeneous Photocatalyst for Photo-induced Atom Transfer Radical Polymerization (ATRP) under UV/vis/NIR and its Application for the Preparation of Dual Mode Curing Injectable Photoluminescence Hydrogel.

The utilizing light with broadband range has attracted lots of research interest for the photo induced reversible-deactivation radical polymerization (RDRP). However, it is still a challenge for a single catalyst to simultaneously respond to various lights with highly varied wavelengths. Here, we proposed a simple strategy for the preparation of a heterogeneous photocatalyst suitable for photo induced atom transfer radical polymerization (photoATRP) under full spectrum (from UV/vis light to NIR), by combining pyridine nitrogen doped carbon dots (N-CDs) and upconversion nanoparticles (UCNPs). In the presence of these robust UCNP@SiO2@N-CDs composite particles, the photoATRP could be carried on under the different irradiations of UV, blue, green, red, white, and 980 nm NIR light, with a low loading of part per million concentrations of the CuBr2/L catalyst. Moreover, the excellent solvent and aqueous compatibility allow UCNP@SiO2@N-CDs to be capable for photoATRP in both organic solvents and aqueous media, providing well-defined hydrophobic and hydrophilic polymers with low dispersity and excellent chain-end fidelity. In addition, the photoATRP with 980 nm NIR exhibited excellent penetrations through visible-light-proof barriers. The system could be used for the preparation of an injectable hydrogel that had dual curing and photoluminescence modes. Owing to the "living" characteristics of polymer chains achieved through ATRP, the hydrogel was capable to be easily repaired by using monomer as the binder.

Miniaturized antithrombin III affinity monolithic columns coupled to TOF-MS for the selective capture and release of fondaparinux a high affinity antithrombin III ligand.

This work explores the capability of antithrombin III-functionalized capillary monolithic columns (in-line coupled with MS detection) to selectively capture, release and detect high affinity binders of antithrombin III (AT III) from oligosaccharides mixtures. The in-situ characterization of home-made AT III affinity columns was done by frontal affinity chromatography coupled to MS detection using fondaparinux as model ligand. Three different preparation methods of miniaturized antithrombin III monolithic affinity columns were optimized and compared. Immobilization of antithrombin III onto Concanavalin A functionalized column is the simplest method but leads to lowest protein density. The two other methods, direct grafting on aldehyde preactivated monoliths and immobilization of biotinylated antithrombin III to streptavidin-functionalized columns, require the presence of fondaparinux to protect the heparin binding site during the grafting process. Up to 1.3±0.3pmolcm-1 of antithrombin III were immobilized with both methods. The direct coupling of such miniaturized affinity columns to MS-detection was made possible by optimization of the elution step. Ammonia (0.1M) was chosen as an efficient and MS compatible solvent to elute fondaparinux. Finally, the complete analytical workflow (capture/elution/detection) was demonstrated to allow the selective capture and elution of fondaparinux initially contained in a complex oligosaccharide mixture with a limit of detection of 1pmol.

Characteristic and complementary chiral recognition ability of four recently developed immobilized chiral stationary phases based on amylose and cellulose phenyl carbamates and benzoates.

To date, various immobilized chiral stationary phases (CSPs) have been developed. The immobilized CSPs have opened up possibilities not only maintaining the high chiral recognition abilities as well as corresponding coated ones but also affording high durability to various mobile phase. This report directed to investigate enantioseparation of recently launched four immobilized CSPs with cellulose and amylose backbones under normal phase liquid chromatography conditions. Their chiral recognition abilities were compared with previously developed six immobilized CSPs. Particularly, we focused on the complementarity for chiral recognitions. Among them, amylose tris(3‐chloro‐5‐methylphenylcarbamate) CSP, namely, CHIRALPAK IG, showed notable chiral recognition abilities to various racemates. As expected, the investigated immobilized CSPs represented remarkable durability to wide range of mobile phases, whereas the corresponding coated CSPs could not be run due to the irreversible degradation. Taking advantage of unrestricted solvent compatibility, chiral separation selectivities were improved for some racemates.

Experimental evaluation of designed and synthesized Alkaline-Surfactant-polymer (ASP) for chemical flooding in carbonate reservoirs

More than half of the oil and gas resources of the world are hosted in carbonate reservoirs. Also, a total of 60% of their residual oil is left unswept during conventional primary and secondary oil recovery operations. The complexity of carbonate reservoirs due to relative weak water imbibition, undesired capillary forces, low permeability (less than 50mD), oil wetness and geochemical interactions in the presence of formation water high salinity causes precipitation of the injection chemicals resulting in clogging of pore throats and residual oil entrapment. Therefore, taking advantage of purposefully designed chemicals known as alkaline-surfactant-polymer (ASP) for enhanced oil recovery (EOR) applications rather than common commercial ASP materials for these challenging petroleum reservoirs is expected. In this research, the synthesized ASP combination was considered to improve the oil production mechanisms of the desired carbonate porous medium. Ultra-low interfacial tension about 1.6 × 10–3 has been achieved by Alkaline-Surfactant-Nano (ASN) synergistic formulation. Meanwhile, wettability alteration was evaluated by contact angle measurement. The contact angle was reduced from Θ = 155.2° (oil-wet) to Θ= 23.7° (water-wet) by the ASN combination. As well, the remarkable HTHS tolerable and shear resistance synthesized Co-polymer Nanocomposite was applied, and 10 times diluted seawater (losal) was chosen as chemicals compatible aqueous solvent for core flooding experiments. Thus, formulated ASP fluids were synergized by alumina nanoparticles at optimized concentrations of chemicals. Hence, this synthesized ASP is able to improve the residual oil production mechanism and lower the capillary pressure (Pc). 16 chemical flooding scenarios were run on Karanj oil field carbonate outcrop cores; the results of the four successful scenarios with the highest original oil in place (OOIP) recovery percentages are discussed. Experiments results are revealed that the cumulative oil recovery could arrive at 91% under the harsh condition of the reservoir. According to the findings of current research, this ASP formulation is highly recommended for chemical flooding of tight carbonate reservoirs.

Core–Satellite Micelles by a Linear A1B1A2B2 Tetrablock Copolymer

Block copolymers in water or compatible solvents show micelles with various shapes, such as worm-like, vesicles, and spheres. In this study, we prepared core–satellite micelles by employing a polystyrene-block-polyisoprene-block-polystyrene-block-polyisoprene (S1I1S2I2) linear tetrablock copolymer. When a PS-compatible solvent, for example, dimethylacetamide or diethylphthalate, is used, the PI chains form the inside core, while the PS chains become a shell as long as the volume fraction of mid PI1 chains (fPI1) is large enough (0.2) to form a loop configuration, resulting in merging into spherical cores consisting of the PI2 chains. However, at smaller fPI1 (0.08), the short mid PI1 chains cannot merge into the PI2 core and therefore exist separately as small spherical micelles, which are referred to as the “satellite” micelles. Thus, the micelles consist of large central core spheres and smaller outside satellite spherical micelles.

Mixed Solvents in Multilayer Ceramic Capacitors (MLCC) Electronic Paste and Their Effects on the Properties of Organic Vehicle.

The copper end paste used in multilayer ceramic capacitors sintered in nitrogen atmosphere leads to carbon residues of organic vehicles, which leads to a reduction in electrode conductivity and high scrap rate. With an attempt to leave no residue in the sintering, the compatibility of solvents and thickeners should be improved because it has an important influence on the hierarchical volatilization and carbon residue of organic vehicles. In this work, the volatility of different solvents was compared, and several solvents were mixed in a definite proportion to prepare an organic vehicle with polyacrylate resins. The hierarchical volatility and solubility parameters of mixed solvents were effectively adjusted by changing proportions of different components. The thermogravimetric curves of resins and organic vehicles were measured by thermogravimetric analyzer, and the effect of solubility parameter on the dissolvability of resins in the solvent and the residual of organic vehicles were studied. Results showed that the hierarchical volatilization of solvents can be obtained by mixing different solvents; the intrinsic viscosity of the organic vehicle is higher, and the thermal decomposition residue of polyacrylate resins is lower when the solubility parameters of mixed solvents and polyacrylate resins are closer. The low residual sintering of organic vehicles can be achieved by using a mixed solvent with hierarchical volatility and approximate solubility parameters as resins.

Simple Approach for Reliable Prediction of Solubility of Polymers in Environmentally Compatible Solvents

This work presents a simple approach for predicting the solubility parameter (δ) of polymers. It is based on the number of moles of hydrogen, nitrogen, oxygen, sulfur, fluorine, and silicon atoms per mole of repeating unit structures as well as two correcting functions based on the contributions of hydrogen bonding and polar groups. The largest available experimental data including the δ values of 163 amorphous polymers and amorphous phases of semicrystalline polymers are used to derive and test the new model. Different statistical parameters are used to confirm the high reliability of the novel method through internal and external validations as well as by comparing with two of the best existing quantitative structure–property relationship (QSPR) approaches containing complex descriptors. Since the available QSPR methods are restricted to polymers with repeating unit structures −(C1H2–C2R3R4)–, the results of the new method are compared with the outputs of QSPR methods for 97 polymers. The value of root-mean-square error (RMSE) of the novel approach for external validation of test data (57 different polymers) is 0.91 MPa1/2, which is close to RMSE values of the computed outputs of two complex QSPR methods, that is, 0.90 and 0.88 MPa1/2.

A selective and accurate liquid chromatography-tandem mass spectrometry method for the quantitation of the novel 5-HT4 receptor partial agonist SUVN-D4010 (Usmarapride) in human plasma and urine

Liquid chromatography and the tandem mass spectrometry method to quantitate SUVN-D4010 (Usmarapride) in human plasma and urine have been developed and fully validated in compliance with regulatory guidelines. The sample preparation technique is simple and rapid consisting of acetonitrile precipitation followed by dilution of supernatant with a compatible solvent. Chromatographic separation was achieved on an X-Bridge C18 (2.1×50 mm, 3.5 µm) column using 0.1% v/v ammonium hydroxide and acetonitrile as mobile phase components, delivered at a flow rate of 0.75 mL min−1. Electrospray Ionization technique in positive mode was used for mass spectrometric detection. Selective reaction monitoring (SRM) transitions of m/z 384.2 → 352.1 for SUVN-D4010 and m/z 388.2 → 356.1 for SUVN-D4010-d4 were used for quantitation. Calibration curves for SUVN-D4010 were linear across the concentration range of 0.3–300 ng mL−1 in human plasma and 5.00–5000 ng mL−1 in human urine. The method generated results with acceptable accuracy (± 9.0%), precision (%CV, ≤8.7), and mean extraction recovery (≥93.4%) with negligible matrix effect in both plasma and urine. SUVN-D4010 was found to be stable in human plasma and urine at the defined storage conditions. The validated method was successfully applied to quantitate SUVN-D4010 in human plasma and urine from a clinical first-in-human study conducted to evaluate its safety, tolerability, and pharmacokinetics in healthy adults.