Nanoparticles In Solution Research Articles

Understanding the Effect of the Synthetic Method and Surface Chemistry on the Properties of CsPbBr3 Nanoparticles.

Over the last decade, the attractive properties of CsPbBr3 nanoparticles (NPs) have driven ever-increasing progress in the development of synthetic procedures to obtain high-quality NPs at high concentrations. Understanding how the properties of NPs are influenced by the composition of the reaction mixture in combination with the specific synthetic methodology is crucial, both for further elucidating the fundamental characteristics of this class of materials and for their manufacturing towards technological applications. This work aims to shed light on this aspect by synthesizing CsPbBr3 NPs by means of two well-assessed synthetic procedures, namely, hot injection (HI) and ligand-assisted reprecipitation (LARP) in non-polar solvents, using PbBr2 and Cs2CO3 as precursors in the presence of already widely investigated ligands. The overall goal is to study and compare the properties of the NPs to understand how each synthetic method influences the NPs' size and/or the optical properties. Reaction composition and conditions are purposely tuned towards the production of nanocubes with narrow size distribution, high emission properties, and the highest achievable concentration. As a result, the formation of bulk crystals as precipitate in LARP limits the achievement of a highly concentrated NP solution. The size of the NPs obtained by LARP seems to be poorly affected by the ligands' nature and the excess bromide, as consequence of bromide-rich solvation agents, effectively results in NPs with excellent emission properties. In contrast, NPs synthesized by HI exhibit high reaction yield, diffusion growth-controlled size, and less striking emission properties, probably ascribed to a bromide-deficient condition.

(Invited) Direct Laser Induced Gold Wiring of 2D Materials with Sub-Μm Resolution

One of the most influential technologies of our modern world are integrated electronics. Almost all parts of our daily life include some bearing with any kind of electrical technologies. The fabrication methods itself, for integrated miniatured electrical devices have had not much innovation in terms of production. Several processing steps still mainly consist of different kinds of lithography. This situation may change with new challenges on the production of smaller quantities with specialized functions, or the use of temperature critical or atomically thin surfaces, where the thermal evaporation of metals damages the sample.For some decades now, printing of all types of electronics have gained great attraction. While lithography is a time-consuming multi-step process (including resist preparation, illuminating, developing, evaporation and lift-off) with expensive and harmful photoresists and harsh environmental conditions (high-vacuum and thermal treatments), ink printing allows for direct and simple application of the desired structures [1]. Two main types of inks are typically applied: colloidal solutions of metal nanoparticles [2, 3] or metal-organic precursor solutions [4]. In both cases, thermal curing after deposition is required to achieve metallic conduction. To this end, laser writing is often advantageous over thermal sintering due to the overall lower thermal stress applied to the material, particularly on flexible, thermally sensitive substrates [5] whereas metal nanoparticles suffer from a relatively high photothermal stability and the need for a large laser power to achieve sufficient sintering [3]. In contrast, metal-organic precursor solutions feature a relatively low metal content (≤34 wt-%)10, and undergo large volume contractions during laser writing. These challenges have so far prevented the definition of metallic gold contacts with diffraction-limited feature sizes by ink printing and laser writing.Here, we introduce atomically-precise, metalloid Au32(nBuP12Cl8) nanoclusters (Au32NCs) [6] as an ink for resistless, direct laser writing of metallic gold micro- and nanostructures. The unique advantages of this ink over Au nanoparticles or typical metal-organic Au precursors are their high photosensitivity in combination with a large gold content of 70 wt-%. This allows for writing gold structures with feature sizes of 250 nm and line spacings < 200 nm using 1.86 mW laser power at 488 nm with a scan speed of 0.1 mm/s. We apply this method on a wide range of substrates, including flexible polymer membranes, find metallic conductance with conductivities ~ 1 × 106 S/m and demonstrate that defining such Au contacts on two-dimensional semiconductors results in fully functional optical switches and field effect transistors made out from 2D materials.[1] Raut NC. et al., Jour. Mat. Chem. C 2018, 6(7): 1618-1641.[2] Ko E. et al., Nanotechnology 2007, 18(34): 345202.[3] Hong S. et al., ACS Nano 2013, 7(6): 5024-5031.[4] Schoner C et al., Thin Solid Films 2013, 531: 147-151.[5] Skylar-Scott MA et al., PNAS 2016, 113(22): 6137-6142.[6] Kenzler S et al., Angew. Chem. Int. Ed. 2019, 58(18): 5902-5905. Figure 1

Green Synthesis Silver Nanoparticles Using Sembung (Blumea balsamifera) Leaf Extract as an Antibacterial and Antioxidant

Silver Nanoparticles essentially focuses on the synthesis of nano-sized particles produced through chemical, physical and biological processes, which contribute significantly to the control of plant and animal diseases and have shown considerable promise in improving the quality of living conditions and human health. Currently, silver nanoparticles (AgNP) have shown their potential as an alternative antibacterial and antioxidant agent in many studies. The method for making silver nanoparticles, namely Green synthesis, involves the use of plants for the synthesis process of various types of nanoparticles. Green synthesis has the advantages, namely that the method is simple, environmentally friendly, non-polluting, antitoxic and cost-effective. The aim of this research is to determine the antibacterial and antioxidant activity of AgNPs. In this research, the nanoparticle solution was visually determined by the yellow-brown color change. In the spectrophotometer absorption spectrum appears at a wavelength of 425 nm. The results of determining the size distribution of silver nanoparticles using PSA show that the particle size distribution obtained has an average value of 40.5 nm and 59.6 nm for silver nanoparticles for AgNO3 concentrations of 1 mM and 2 mM, respectively. FTIR measurements are used to determine the presence of bioactive molecules that may be responsible for the stabilization that acts as a capping agent. Absorption spiked at 3.280; 2.919; 1.583; 1.379; 1.239 and 1.068 cm−1 were determined for sembung leaf extract, while silver nanoparticles showed an absorption spike at 3.368; 2.850; 1.594; 1.369; 1.244 and 1.108 cm−1. The results of XRD analysis of AgNP show that it has been successfully synthesized which can be seen from the formation of a narrow peak indicating the crystalline nature of the nanoparticles formed. The results of TEM analysis of AgNPs in this study are mixed shapes such as spherical, hexagonal and triangular shapes of silver nanoparticles. The antibacterial activity test of silver nanoparticles with sembung leaf extract with varying concentrations of AgNO3 solution was successful as indicated by the formation of an inhibition zone for Eschericia coli and Staphylococcus aureus bacteria. The results show that the antioxidant activity of AgNPs shows that the percentage of inhibition increases as the concentration of AgNPs increases from 100 to 500 ppm and ascorbic acid increases from 1 to 5 ppm.

Effect of Aspect Ratio of a Gold-Nanorod-Modified Screen-Printed Carbon Electrode for Carbaryl Detection in Three Different Samples of Vegetables.

In this study, three different sizes of gold nanorods (AuNRs) were synthesized using the seed-growth method by adding various volumes of AgNO3 as 400, 600, and 800 μL into the growth solution of gold nanoparticles. Three different sizes of AuNRs were then characterized using UV-vis spectroscopy, high-resolution transmission electron microscopy (HRTEM), selected area electron diffraction (SAED) patterns, and atomic force microscopy (AFM) to investigate the surface morphology, topography, and aspect ratios of each synthesized AuNR. The aspect ratios from the histogram of size distributions of three AuNRs as 2.21, 2.53, and 2.85 can be calculated corresponding to the addition of AgNO3 volumes of 400, 600, and 800 μL. Moreover, each AuNR in three different aspect ratios was drop-cast onto the surface of a commercial screen-printed carbon electrode (SPCE) to obtain three different SPCE-modified AuNRs (SPCE-A400, SPCE-A600, and SPCE-A800, respectively). All SPCE-modified AuNRs were then evaluated for their electrochemical behavior using cyclic voltammetry and electrochemical impedance spectroscopy (EIS) techniques and the highest electrochemical performance was shown as the order of magnitude of SPCE-A400 > SPCE-A600/SPCE-A800. The reason for the highest electrocatalytic activity of SPCE-A400 might be due to the smallest particle size and uniform distribution of AuNRs ∼ 2.2, which enhanced the charge transfer, thus providing the highest electroactive surface area (0.6685 cm2) compared to other electrodes. These results also confirm that the sensing mechanism for all SPCE-modified AuNRs is controlled by diffusion phenomena. In addition, the optimum pH was obtained as 4 for carbaryl detection for all SPCE-modified AuNRs with the highest current shown by SPCE-A400. Furthermore, SPCE-A400 has the highest fundamental parameters (surface coverage, catalytic rate constant, electron transfer rate constant, and adsorption capacity) for carbaryl detection, which were investigated using cyclic voltammetry and chronoamperometric techniques. The electroanalytical performances of all SPCE-modified AuNRs for carbaryl detection were also investigated with SPCE-A400 displaying the best performance among other electrodes in terms of its linearity (0.2-100 μM), limit of detection (LOD) ∼ 0.07 μM, and limit of quantification (LOQ) ∼ 0.2 μM. All SPCE-modified AuNRs were also subsequently evaluated for their stability, reproducibility, and selectivity in the presence of interfering species such as NaNO2, NH4NO3, Zn(CH3CO2)2, FeSO4, diazinon, and glucose and show reliable results as depicted from %RSD values less than 3%. At last, all SPCE-modified AuNRs have been employed for carbaryl detection using a standard addition technique in three different samples of vegetables (cabbage, cucumber, and Chinese cabbage) with its results (%recovery ≈ 100%) within the acceptable analytical range. In conclusion, this work demonstrates the great potential of a disposable device based on an AuNR-modified SPCE for rapid detection and high sensitivity in monitoring the concentration of carbaryl as a residual pesticide in vegetable samples.

A novel dye-sensitised solar cell based portable sensor for chemical detection

In recent years, chemical sensors have shown the potential to provide rapid, accurate, and on-site quantitative detection of analytes, but current methods often require specialised equipment. In this study, a novel dye-sensitised solar cell (DSSC) based sensor is reported for convenient detection of tert-butylhydroquinone (TBHQ) in drinking water. The sensor consists of a gold nanoparticle (AuNP) solution based unit that responds to the concentration of TBHQ and produces an electrical signal change via an integrated DSSC based transducer component. Through analysing the current signal generated by the DSSC sensor, the quantity of TBHQ in an unknown sample can be achieved. Finite difference time domain (FDTD) simulation has been used to explain the relationship between the particle morphologies and optical properties of AuNPs. This work offers a novel strategy of developing portable sensors without needing any scientific instrument.

Structural characterization of marine macroalgae derived silver nanoparticles and their colorimetric sensing of hydrogen peroxide

The present study investigates the algae-mediated synthesis, characterization, and H2O2 sensing ability of silver nanoparticles using Sargassum spp. extract. The synthesized nanoparticles were spherical agglomerates and exhibited an absorbance peak at 418 nm, as analyzed by FE-SEM and UV–vis spectrophotometric analysis. TEM-SAED analysis interpreted the size between 2 and 35 nm and crystallinity corresponding to face-centered cubic (FCC) silver structure. This was further supported from XRD findings, which ascertained the presence of (111), (200), (220), and (311) lattice planes. FT-IR analysis confirmed the capping of algal phenolics as corresponding functional groups, which was further confirmed by XPS studies. The nanoparticles were observed to be highly stable in aqueous suspensions, due to the zeta potential value (−22.6 mV). On incubation of synthesized nanoparticles, with H2O2 solutions, decolorization of nanoparticle solution was observed along with the reduction of absorbance peak at 418 nm. This color change could be utilized for H2O2 detection in contaminated samples. Therefore, this study reveals the potential of sustainable Sargassum spp. biomass in synthesizing beneficial silver nanoparticles which could be exploited for environmental detection applications.

Experimental Water Activity Suppression and Numerical Simulation of Shale Pore Blocking

The nanoscale pores in shale oil and gas are often filled with external nanomaterials to enhance wellbore stability and improve energy production. And there has been considerable research on discrete element blocking models and simulations related to nanoparticles. In this paper, the pressure transmission experimental platform is used to systematically study the influence law of different water activity salt solutions on shale permeability and borehole stability. In addition, the force model of the particles in the pore space is reconstructed to study the blocking law of the particle parameters and fluid physical properties on the shale pore space based on the discrete element hydrodynamic model. However, the migration and sealing patterns of nanomaterials in shale pores are unknown, as are the effects of changes in particle parameters on nanoscale sealing. The results show that: (1) The salt solution adopts a formate system, and the salt solution is most capable of blocking the pressure transmission in the shale pores when the water activity is 0.092. The drilling fluid does not easily penetrate into the shale pore space, and it is more capable of maintaining the stability of the shale wellbore. (2) For the physical blocking numerical simulation, the nanoparticle concentration is the most critical factor affecting the shale pore blocking efficiency. Particle size has a large impact on the blocking efficiency of shale pores. The particle diameter increases by 30% and the pore-blocking efficiency increases by 13% when the maximum particle size is smaller than the pore exit. (3) Particle density has a small effect on the final sealing effect of pore space. The pore-plugging efficiency is only increased by 4% as the particle density is increased by 60%. (4) Fluid viscosity has a significant effect on shale pore plugging. The increase in viscosity at a nanoparticle concentration of 1 wt% significantly improves the sealing effectiveness, specifically, the sealing efficiency of the 5 mPa-s nanoparticle solution is 16% higher than that of the 1 mPa-s nanoparticle solution. Finally, we present a technical basis for the selection of a water-based drilling fluid system for long horizontal shale gas drilling.

Acute toxicity of zinc carbonate nanocrystals on white mice model

Zinc-based nanoparticles (NPs) are successfully used to meet the mineral needs of animals and poultry, capable of increasing the growth rate and the efficiency of their use of feed. Nanoparticles of zinc oxide are widely used, which are superior to conventional sources of zinc in terms of pharmacological activity and bioavailability, have a positive effect on productivity and antioxidant protection, and increase reproductive potential. However, a detailed investigation of their toxicological parameters showed the risks of their introduction into the body, as a result of which a large number of zinc oxide NPs were synthesized by the methods of “green chemistry”, the main drawback of which is a complex methodology and a high level of costs. We investigated the acute toxicity of a colloidal solution of zinc carbonate nanocrystals in a model of white mice. The experiment was conducted on 42 female non-linear white mice, 3–4 months old, with a live weight of 21–23 g, kept under optimal vivarium conditions. According to the principle of analogues, 6 experimental groups were formed: mice were injected with a colloidal solution of zinc carbonate nanoparticles in doses (by absolute weight of the drug) of 15,000.0; 20000.0; 25000.0; 30000; 35000.0 and 40000.0 mg/kg of body weight based on the absolute weight of the drug, which according to the active substance (zinc carbonate nanoparticles) was (37.5; 50.0; 62.5; 75.0; 87.5 and 100, 0 mg/kg of body weight), orally using an esophageal-gastric tube. Mice of the control group were injected with distilled water in a volume of 0.2 cm3 according to the same protocol. The clinical condition of the experimental animals was observed for 14 days, noting the appearance and development of clinical signs of poisoning, the time of death or recovery to the physiological norm. After the death (diagnostic slaughter) of the animals, a pathological autopsy was performed. Clinical observations showed that a single intragastric administration of the drug to mice of experimental groups 1-6 did not cause acute poisoning. The deaths of mice in all experimental groups were not observed during the 14-day observation period, as a result of which the LD50 indicator could not be calculated. At the same time, the maximum possible injected dose of the colloidal solution of zinc carbonate nanoparticles (by absolute weight) was 40,000.0 mg/kg of body weight (100.0 mg/kg by active substance), which allows the drug to be assigned to the VI class of toxicity – substances are relatively harmless (LD50 &gt; 15000.0 mg/kg of body weight), and according to the degree of danger up to IV class – low-hazardous substances (LD50 &gt; 5000.0 mg/kg of body weight).

Carbon-supported Ternary Nanocatalyst Palladium-Vanadium-Cobalt for Hydrodechlorination of 2,4-Dichlorophenol

M ultimetallic nanocatalysts have remarkably revealed activities in various catalytic applications. Herein, the ternary nanocatalysts based on palladium-vanadium-cobalt were successfully synthesized through the reduction of their salts with the combination of sodium borohydride and ethylene glycol as reducing agents, including impregnating of activated carbon (AC) substrate with colloid solution of metallic nanoparticles (PdVCo/C). The immobile of trimetallic PdVCo on AC was evidenced by XRD, EDX analysis, transmission electron microscopy (TEM) and specific surface area (BET). Therein, TEM imaging showed that the PdVCo particles size is about 7 nm. The trimetallic nanocatalysts were evaluated in the hydrodechlorination of dichlorinated substrates which was defined by GC (FID detector). The obtained result was that the conversion of 2,4-dichlorophenol was over 91% in the case of PdVCo nano-catalyst in the presence of potassium hydroxide and the high catalytic stability was observed.

Silica supported biosynthesized silver nanoparticles as effective adsorbent and photocatalyst for removal of methylene blue from water

The silica supported silver nanoparticles (Ag@SG) were prepared by wet impregnation of silica gel (SG) using different concentrations of biogenic silver nanoparticles (AgNPs; 0.25–5 mM) solution, prepared from Cicer arientinum pod extract. Among them, the Ag@SG composite prepared by using 1.25 mM AgNPs solution (Ag1.25@SG) was found to contain only Ag0 species as nanoparticles, whereas the samples prepared by higher concentrations contained Ag0 as well as Ag+ (silver oxide) species. All the Ag@SG composites including SG, exhibited good adsorption capacity for a cationic dye (methylene blue; MB) in water. However, Ag1.25@SG having only Ag0 species exhibited higher photocatalytic activity for degradation of MB under visible light irradiation as compared to other composites. The study reveals that the plasmonic Ag0 species supported on silica are catalytically more active than Ag+ species. Furthermore, the AgNPs (Ag0) in supported form exhibits much higher (∼5-fold) photocatalytic activity than unsupported AgNPs. The synergistic effect of adsorption property of silica surface and plasmonic photocatalytic activity of AgNPs in the synthesized composites was found to be effective for the removal of MB. In addition, the fine dispersion of AgNPs on internal surface of silica provides better activity for photocatalysis. The study shows that this material is a promising adsorbent as well as photocatalyst for the removal of dyes from water.

Modern Trends in Oral Biology Research

Modern Trends in Oral Biology Research

AN IMPACT OF THE LASER IRRADIATION TIME ON PROPERTIES OF COLLOIDAL SOLUTIONS OF SILICON NANOPARTICLES

The design of semiconductor-metallic nanostructures using pulsed laser ablation in liquids (PLAL) is a very demanding task for biomedical applications being at an early stage of its development. Only few recent papers show the possibility of such a synthesis of composite nanoparticles as well as their perspectives for biosensing applications. However, mechanisms of the laser-stimulated formation of semiconductor-metallic nanoparticles involving several processes are not clarified yet being considerably depended on experimental conditions. In this work, we demonstrated an impact of the laser irradiation of colloidal solutions of silicon nanoparticles at different exposure time in the presence/absence of a gold target. In particular, longer ablation of the metal led to a stronger plasmonic maximum in silicon nanoparticles at around 520 nm. It also decreased the hydrodynamic size from 165 nm to 85 nm as well as the ξ-potential from –46 mV to –30 mV by increasing the ablation time from 0 s to 600 s. At the same time, the lowest electrical conductivity value (~1.5 µS/cm) of the plasmonic nanocomposites was detected at 120 s irradiation time. The highest concentration of synthesized composite nanoparticles (~3·1011 NPs/mL) was achieved at 180 s ablation time. Another purpose of the paper was to reveal an influence of the used laser irradiation on properties of the colloidal solutions of silicon nanoparticles themselves. It was found a considerable decrease of their absorbance with the increase of the laser exposure time that can be associated with the change of their properties (e.g. concentration, size, oxidation state etc.). Thus, the laser irradiation strongly affects properties of colloidal solutions of silicon nanoparticles that must also be taken into account considering possible mechanisms of the formation of composite nanostructures. Presented in the paper fast optical diagnostic can help to determine properties of colloidal solutions of nanocomposites formed by PLAL prior their biomedical or catalytic applications.

Titanium dioksit nano partiküllerinin ayçiçeği fide çıkışı üzerine olumlu etkileri

Seed germination and seedling emergence is the main step of cultivation and improving them could yield high performance in the field. Improved seedling emergence means less sensitivity to biotic and abiotic stress factors. It is possible to enhance seedling emergence via different technologies. Nanoparticles are one of the improving technology and their impact on crop cultivation are improving day by day. The seeds of hybrid-snack type cultivar Ahmetbey and for seed treatment agent TiO₂ nanoparticles were used in this experiment. This study was conducted to observe the impact of seed treatment with different titanium dioxide (TiO₂) nanoparticles (NPs) concentrations (6, 12, and 24 mg Lˉ¹) with dimensions of 20-50 nm during 8 hours on the emergence and seedling growth performance of snack-type sunflower cultivar Ahmetbey. Four replicates of 50 seeds in each treatment were sown in plastic trays 4 cm deep and placed in a growth chamber at 20 ± 2 °C 45 μM photons m-² s-¹ light for 16 h. Mean emergence time (MET), emergence percentage, seedling vigor, root-to-shoot length ratio, shoot length, and root length seedling fresh and dry weight were measured. Emergence percentage, shoot length, root length, and fresh and dry weight of seedlings increased with TiO₂ NPs treatments. The results revealed that 8-hour priming with water has a low impact on seeds of cv. Ahmetbey compared to any treatment of TiO₂ NPs. In conclusion, it is proved that the improving effects of 8 hour priming of sunflower seeds with TiO₂ NPs solutions on sunflower seedling emergence.

Experimental Study on SiO2 Nanoparticles-Assisted Alpha-Olefin Sulfonate Sodium (AOS) and Hydrolyzed Polyacrylamide (HPAM) Synergistically Enhanced Oil Recovery

The purpose of this study is to investigate the use of SiO2 nanoparticles in assisting with surfactants and polymers for tertiary oil recovery, with the aim of enhancing oil recovery. The article characterizes the performance of SiO2 nanoparticles, including particle size, dispersion stability, and zeta potential, evaluates the synergistic effects of nanoparticles with alpha-olefin sulfonate sodium (AOS) surfactants and hydrolyzed polyacrylamide (HPAM) on reducing interfacial tension and altering wettability, and conducts core flooding experiments in rock cores with varying permeabilities. The findings demonstrate that the particle size decreased from 191 nm to 125 nm upon the addition of SiO2 nanoparticles to AOS surfactant, but increased to 389 nm upon the addition of SiO2 nanoparticles to HPAM. The dispersibility experiment showed that the SiO2 nanoparticle solution did not precipitate over 10 days. After adding 0.05% SiO2 nanoparticles to AOS surfactant, the zeta potential was −40.2 mV, while adding 0.05% SiO2 nanoparticles to 0.1% HPAM resulted in a decrease in the zeta potential to −25.03. The addition of SiO2 nanoparticles to AOS surfactant further reduced the IFT value to 0.19 mN/m, altering the rock wettability from oil-wet to strongly water-wet, with the contact angle decreasing from 110° to 18°. In low-permeability rock core oil displacement experiments, the use of AOS surfactants and HPAM for enhanced oil recovery increased the recovery rate by 24.5% over water flooding. The recovery rate increased by 21.6% over water flooding in low-permeability rock core experiments after SiO2 nanoparticles were added and surfactants and polymers were utilized for oil displacement. This is because the nanoparticles blocked small pore throats, resulting in increased resistance and hindered free fluid flow. The main causes of this plugging are mutual interference and mechanical entrapment, which cause the pressure differential to rise quickly. In high-permeability rock core oil displacement experiments, the use of AOS surfactants and HPAM for oil recovery increased the recovery rate by 34.6% over water flooding. Additionally, the recovery rate increased by 39.4% over water flooding with the addition of SiO2 nanoparticles and the use of AOS surfactants and HPAM for oil displacement. Because SiO2 nanoparticles create wedge-shaped structures inside highly permeable rock cores, they create structural separation pressure, which drives crude oil forward and aids in diffusion. This results in a comparatively small increase in pressure differential. Simultaneously, the nanoparticles change the rock surfaces’ wettability, which lowers the amount of crude oil that adsorbs and improves oil recovery.

High-Precision Optical Excited Heaters Based on Au Nanoparticles and Water-Soluble Porphyrin

Gold nanoparticles are widely used as local heaters under optical excitation. Hybrid molecular-plasmon nanostructures based on gold nanoparticles and water-soluble porphyrin have been developed. A colloidal solution of gold nanoparticles was obtained by laser ablation of metallic gold in water, ensuring its highest chemical purity. The hybrid nanostructures formation was performed due to the Coulomb interaction of cationic porphyrin and gold nanoparticles. The revealed functional properties of hybrid nanostructures make them promising for controllable nano-heater applications (for example, photothermal therapy). Gold nanoparticles act as heaters, whereas porphyrin serves as a fluorescent thermometer with a single optical excitation.

Multivariate Analysis of Protein-Nanoparticle Binding Data Reveals a Selective Effect of Nanoparticle Material on the Formation of Soft Corona.

When nanoparticles are introduced into the bloodstream, plasma proteins accumulate at their surface, forming a protein corona. This corona affects the properties of intravenously administered nanomedicines. The firmly bound layer of plasma proteins in direct contact with the nanomaterial is called the "hard corona". There is also a "soft corona" of loosely associated proteins. While the hard corona has been extensively studied, the soft corona is less understood due to its inaccessibility to analytical techniques. Our study used dynamic light scattering to determine the dissociation constant and thickness of the protein corona formed in solutions of silica or gold nanoparticles mixed with serum albumin, transferrin or prothrombin. Multivariate analysis showed that the nanoparticle material had a greater impact on binding properties than the protein type. Serum albumin had a distinct binding pattern compared to the other proteins tested. This pilot study provides a blueprint for future investigations into the complexity of the soft protein corona, which is key to developing nanomedicines.

Simulation of noble metal nanocluster systems formation during deposition from a colloid solution

A model was proposed for the convective flow of the liquid phase of a colloidal solution of glycerol and noble metal nanoparticles (Ag, Au, Ag/Au) near the substrate. The diffusion approximation is used to describe the formation of nanocluster systems on a substrate. The diffusion-limited aggregation model was implemented by applying a cellular automaton in the Neumann neighborhood. A diverse structure of model systems of nanoclusters, which adequately describes the structural features of the experimental samples, was obtained by varying the aggregation probability parameter. The proposed models can be useful for calibrating the parameters of the experimental production of systems of noble metal nanoclusters, as well as describing in the first approximation the processes that have a decisive effect on nanocluster structures.

Combined application of SiO2 and TiO2 nanoparticles enhances growth characters, physiological attributes and essential oil production of Coleus aromatics Benth

Nanoparticles (NPs) have gained considerable interest among researchers in the field of plant biology, particularly in the agricultural sector. Among the numerous NPs, the individual application of silicon (Si) or titanium (Ti), in their oxide forms, had a positive influence on growth, physiochemical and yield attributes of plants. However, the synergetic application of both these NPs has not been studied yet. Therefore, the current study was aimed to investigate the effect of combined application of silicon dioxide (SiO2) and titanium dioxide (TiO2) NPs on the growth characters, physiological parameters, and essential oil quality and production of Coleus aromatics Benth. Aqueous solutions of nanoparticles were applied to the foliage of the plants at varying combinations (Si50+Ti50, Si100+Ti50, Si100+Ti100, Si200+Ti100, Si100+Ti200 and Si200+Ti200 mg L−1). Various morpho-physiological, biochemical and yield attributes were assessed at 120 days after planting. The results demonstrated that both Si and Ti NPs improved the growth and photosynthetic efficiency in a dose dependent manner. The best results were obtained by the combined application of Si100+Ti100 mg L−1, and thereafter, the values declined progressively. The maximum improvement in fresh weight (39.5 %) and dry weight (40.8 %) of shoot, fresh weight (45.7 %) and dry weight (49.4 %) of root was observed as compared to respective controls. Moreover, the exogenous application of Si100+Ti100 mg L−1 increased photosynthetic attributes such as total content of chlorophyll (41.7 %), carotenoids (43.7 %), chlorophyll fluorescence (7.1 %), and carbonic anhydrase (23.8 %). All of these contributed to the highest accumulation in the content (129.0 %) and yield (215.5 %) of essential oil (EO), in comparison to the control. Thus, results encouraged the use of SiO2 and TiO2 NPs to be applied in combined form to boost the essential oil production of Coleus aromaticus. The findings of this study may serve agronomists to determine the optimal concentrations of NPs for enhanced production of bioactive compounds with a wide range of industrial applications.

Detection of the effect of silver nanoparticles prepared by using extract leaves Prosperis juliflora on pathogenic fungus Trichophyton tonsurans isolated from human scalp

The silver nanoparticles were prepared using Prosopis juliflora leaf extract and extracted from trees in Kirkuk city and showed that the silver nitrate solution in 1 mm turned its color brown which will be preliminary evidence of the formation of nanoparticles. The UV and visible absorption spectrum of a solution of silver nanoparticles was tested as a second step in confirming the formation of the particles, and it was found that they were present at the wavelength (440) nm, where it turned out that the peaks of X-ray diffraction were at (111), (220), (311) at angles (31.77, 48.11, 54.21) respectively. Trichophyton tonsurans were isolated from the scalp of a person with ringworm from Kirkuk General Hospital. The fungus was diagnosed on the basis of the phenotypic and microscopic characteristics and hair penetration. it was found that the solution of silver nanoparticle had a clear inhibitory effect on the fungus Trichophyton tonsurans. The highest and lowest inhibitor concentration reached 90% and the highest inhibitor concentration was 100 % Also, the ratios of the silver nanoparticles effects on the growth and production of fungi spores were determined.

Detected Genetic Markers for Three Varieties of Rice (Oryza sativa L.) under Nano- Particles

Despite the enormous advancements in breeding, fresh and accessible methods of plant development are preferred. Many research have been done to determine the impact of zinc oxide nanoparticles (ZnO NPs) and magnesium nanoparticles (MgONPs) on plant growth. ZnO NPs and MgONPs have been used extensively in agriculture. Few research have looked into the potential impact of ZnO NPs on grain Zn content or cereal yield formation to date. The goal of the study was to demonstrate how varied concentrations of nanoparticles affected root germination in three different rice types (Giza 177, Sakha super 300, and Sakha 108) and the fungus Fusariummoniliforme, which causes foot rot in rice. Here, we performed a pot experiment to assess the effects that ZnO nanoparticles and MgONPs had on the shoot length, root length, and germination of rice. The experiment involved three dosages of Zn and Mg nanoparticle solution at varying concentrations of 10, 20, and 100 ppm. The outcomes showed that, when compared to the control treatment, ZnO NPs and MgONPs increased the length of the shoot, the length of the roots, and the rate of germination. Using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers, the genetic makeup of plants treated with 10, 20, and 100 ppm AgNPs was also examined. In vitro rooted shoots were acclimated in a greenhouse before being tested for biochemical stability and phenotypic stability. In vitro, callogenesis and caulogenesis were both reduced by AgNPs at the greatest dose (100 ppm). In order to maximise genome coverage, three rice cultivars from distinct origins were chosen, and they were tested using three RAPD markers and seven selected ISSR markers. AgNPs-treated plants using ISSR markers produced 87 amplified bands in total, 45 polymorphic allelic variants, and 36 monomorphic allelic variations. The Pearson correlation coefficient was used in the Mantel test to determine whether there was a significant association between the Jaccard's dissimilarity matrices based on ISSR and RAPD markers (r = 0.69; P 0.05). Morphological characteristics, ISSR, and RAPD analysis were used to partition the UPGMA Dendrogram into two groupings. Jaccard's coefficient was used to analyse the genetic similarity matrix. Sakha Super 300 and Giza 177 had the most genetic similarity (95%) while Sakha 108 and Giza 177 had the lowest genetic similarity (90%) according to ISSR and RAPD study. The classification of rice germplasm, breeding initiatives, and conservation efforts all heavily rely on the determination of genetic diversity within the rice species. To find genetic variations, morphological characteristics, ISSR, and RAPD analysis are useful techniques. The findings demonstrated the large ratio of variation in rice. This work successfully established the possibility of using MgONPs and ZnO NPs as high-performing fertilisers to improve rice output and quality.This study could serve as a guide for future research on rice and could support efforts to understand the species and improve breeding stock.