Potassium Permanganate Solution Research Articles

The Effect of TEAT PROTECT spray and potassium permanganate teat dip in curing subclinical mastitis in crossbred cows of Villupuram district of Tamilnadu

A study was undertaken to assess the incidence of subclinical mastitis in crossbred cows of Villupuram district by using direct microscopic Somatic cell count (SCC). For this purpose sixty eight crossbred cows positive for subclinical mastitis (SCM) as per IDF (International Dairy Federation) criteria were randomly allotted in to two treatment groups. The cows in T1 group were subjected to post milking teat dip with one per cent Potassium permanganate (KMnO4) solution immediately after milking. The cows in T2 group were sprayed with TEAT PROTECT sprays. Sixteen healthy cows were kept as control group and udder was washed with plain water. The treatments were carried out for 30 days. Udder health status was determined by recording the parameters viz. somatic cell count (SCC), average daily milk yield, udder colony count and teat colony count. The results revealed that TEAT PROTECT spray is superior to Potassium permanganate in curing SCM of crossbred cows.

Electrophysical Characteristics of Atmospheric Pressure Discharge with Aqueous Potassium Permanganate Solution as Cathode

Electrophysical parameters of atmospheric pressure discharge with an aqueous potassium permanganate solution as a cathode have been determined. The cathode potential drop, the electric field strength in the positive column of the discharge, and the current density depending on the solution concentration and the discharge current have been found, as well as the emission coefficient γ .

Effect of Oxidizing Decontamination Process on Corrosion Property of 304L Stainless Steel

Corrosion behaviors of 304L stainless steel (SS) and 304L SS with oxides film (preoxidation 304L SS) in 1 g/L potassium permanganate solution of various pH values were investigated by using mass loss, electrochemical measurement and scanning electron microscope (SEM) observation. The results showed that mass loss of 304L SS increases with the increase of sodium hydroxide or nitric acid concentration in 1 g/L potassium permanganate solution. The polarization curves of 304L SS in potassium permanganate solution show that passive zones are destroyed more easily in acid potassium permanganate solution than alkaline potassium permanganate solution. The corrosion ability of acid potassium permanganate (NP) decontamination solution used for 304L SS is more aggressive than alkaline potassium permanganate (AP) solution. The oxide film on the surface of preoxidation 304L SS can be removed completely in two oxidation reduction decontamination cycles, oxidizing solution of which comprised 0.4g/L sodium hydroxide and 1g/L potassium permanganate. The 304L SS and preoxidation 304L SS performed alkaline oxidation reduction decontamination of 3 cycles were reoxidation. The micromorphology of reoxidation specimens was similar to preoxidation 304L SS. Therefore the chemical decontamination of alkaline oxidizing and acid reducing steps had no negative effect on corrosion of 304L SS and reoxidation of 304L SS carried out decontamination.

Some Observations on Thin Layer Chromatography Technique

The present study is to report the various problems faced during TLC methodology. Although used regularly some technical aspects must be kept in mind to get better and uniform results. During our experiments with TLC methods we came across some problems and here these aspects of TLC methodology are being highlighted. It is suggested that the solvent use as mobile phase should also be used for extraction of any particular phytochemical. TLC plates should be 3 to 4 mm thick, to be dried for at least 72 hrs. It is also suggested that potassium permanganate solution gives better clarity in visualizing the spots.

EFFECT OF CHEMICAL TREATMENTS ON HARDNESS AND TOUGHNESS PROPERTIES OF GREWIA SERRULATA REINFORCED POLYMER COMPOSITES

Natural fiber reinforced composite materials were manufactured by reinforcing Grewia Serrulata with unsaturated polyester matrix material. To improve the adhesion between fiber/matrix interphase; fibers were treated with Silane, Acetylation and Potassium Permanganate solutions. Partially biodegradable composite materials were prepared using hand lay-up technique. Toughness and hardness of the materials were investigated according to ASTM standards. Acetylation and Silane treatment of Grewia Serrulata improved the compatibility between the matrix and the fibers and resulted in significant enhancement of hardness and impact strength

Тонкослойная хроматография L-карнитина в мицеллярных и циклодекстриновых подвижных фазах

L-carnitine (lat. Levocarnitinum, eng. Levocarnitine) is a natural substance akin to B vitamins. Normalizing metabolic processes in the human body and having a wide range of therapeutic actions, L-carnitine is widely used in cardiology, neurology, gastroenterology, and sports medicine. Currently, modern analytical techniques, such as mass spectrometry, high performance liquid chromatography, radiation methods, etc., are used to analyze L-carnitine. Most of them are rather expensive and time consuming. Thin-layer chromatography (TLC), which is notable for its simplicity, feasibility of analysis, separation efficiency and low cost, has received much less use. In this regard, the purpose of this work was to reveal the analytical capabilities of water-organic, micellar and cyclodextrin mobile phases for the analysis of L-carnitine by means of TLC. Initial L-carnitine solutions with a concentration of 20 mg/ml were prepared by diluting solutions for injections. Their chromatography was carried out by ascending thin-layer chromatography on plates of various polarities (Sorbfil, Plasmachrom, and RP-18) with a fixed sorbent layer. Aqueous solutions of surfactants (cetyltrimethylammonium bromide, cetylpyridinium chloride, sodium dodecyl sulfate, Triton X-100), of cyclodextrins (-cyclodextrin, 2-hydroxypropyl--cyclodextrin), hydroxypropyl--cyclodextrin) were used as mobile phases. The zone of the main substance (L-carnitine) was identified by treating the plate with a mixture of freshly prepared 0.1N solution of potassium permanganate and 2.0 N acetic acid (the zone was colored in a light yellow color). Main features of the chromatographic behavior of L-carnitine were revealed. Based on the calculation of the number of theoretical plates and the height equivalent to one theoretical plate, it was shown that aqueous micellar and cyclodextrin mobile phases allow one to improve the chromatographic process efficiency and the shape of the chromatographic zones of L-carnitine as compared to water-organic eluents; cheaper Sorbfil plates (Russia) can be used for practical purposes. Optimal chromatographic systems and conditions for their use in the thin-layer chromatography of pharmaceuticals and food products have been selected and substantiated.

Pengaruh Perendaman Alkali, Kalium Permanganat, dan Hidrogen Peroksida terhadap Perubahan Diameter Serat Sabut Kelapa Sebagai Bahan Komposit Ramah Lingkungan

This study aims to determine the effect of alkali treatment and hydrogen peroxide on the change in diameter of coconut fiber as an environmentally friendly composite material. This research was carried out with several stages, namely: preparation of materials and tools, treatments, measurement of diameter, analysis, and discussion. Materials and equipment used are coconut fiber, alkaline solution, hydrogen peroxide solution, distilled water, a set of immersion media, and digital microscopes. Coconut fiber was soaked in alkaline solution with 10% concentration for 1, 3, 5, 7, 9, and 11 hours, then dried in a furnace at 90oC for 5 hours. Furthermore, coconut fiber was soaked again in potassium permanganate solution with a concentration of 0.5% for 1, 3, 5, 7, 9, and 11 hours, then dried in a furnace at 90oC for 5 hours. Then the coconut fiber was soaked again in a hydrogen peroxide solution with a concentration of 10% for 1, 3, 5, 7, 9, and 11 hours, then dried in a furnace at 90oC for 5 hours. After that, using a digital microscope the diameter of coconut fiber is measured. Based on the measurement results, it was concluded that the diameter of coconut fiber was degraded in diameter after soaking in alkaline solution, potassium permanganate, and hydrogen peroxide. The highest percentage reduction in diameter was 58.08% in the third treatment.

Characterization of cellulosic fibre from Phoenix pusilla leaves as potential reinforcement for polymeric composites

Phoenix pusilla fibres (PPFs) were extracted from the leaves of a plant belonging to the family of Arecaceae, which is widely grown in Sri Lanka and Southern parts of India for some medicinal purposes. Their use as possible reinforcement in hydrophobic polymeric matrix composites is yet to be studied and for this reason, the better understanding of their microstructure, chemical composition, and mechanical properties becomes essential. In this view, the present investigation deals with the study on the effect of various chemical treatments on the mechanical, chemical structure, thermal and morphological behaviour of PPFs. The chemical treatment of fibres was initially involved with NaOH and then followed by benzoyl peroxide, potassium permanganate and stearic acid solution at different concentrations for the suitable time interval. The results revealed that chemical treatments help in diminishing the amorphous content from the fibres, while the FTIR analysis clearly indicates the removal of many polar impurities. The increase in crystallinity index and crystallite size was seen in all the modified fibres when compared with the raw ones. The improved thermal stability behaviour in all the chemically treated fibres was demonstrated by thermogravimetric and differential scanning calorimetry analysis. The tensile properties of the fibres were analyzed through ASTM standard and finally, the surface morphology was analyzed using scanning electron microscopy. All the favourable results indicated that the PPFs could be used in the applications of natural fibre composites.

A hollow fiber prepared through combining polymethacrylate with manganese oxide as reusable oxidant with valence-selfstabilizing function for cation blue decolorization

By hydrolyzing polymethacrylate hollow fiber in sulfuric acid and then treating it in the aqueous solution of potassium permanganate, we obtained a hollow fibrous oxidant in which in-situ generated manganese oxide was immobilized by partly-hydrolyzed polymethacrylate. The prepared oxidant could oxidize cation blue into colorless substance; thus, it was very efficient at decolorizing cation blue. The results of TOC, FTIR and NMR revealed that the decolorization of cation blue was mainly caused by the cleavage of its double bond of C and N. The oxidant could interact with water to produce oxygen species such as O2·- during decolorization. The oxygen species could help the oxidant to keep its Mn valence unchanged; thus, the oxidant was stable and reusable in decolorizing cation blue. The oxidant could be used up to 5 cycles without any loss in its oxidation activity. It only took less than 30 min to remove 95% of cation blue from 10 mL of its solution with the concentration of 20 mg/L for each cycle.

Accurate, Photoresistor-Based, Student-Built Photometer and Its Application to the Forensic Analysis of Dyes

Student-built photometers are recognized as being useful tools in teaching laboratories that help students understand the concepts behind experimental measurements, and several devices have been reported that employ photoresistors as the detector of choice. We demonstrate here an improvement over these instruments and the analyses thereof, providing more rapid deployment in the laboratory and also considerable increases in both linearity and accuracy. This was confirmed by conducting quantitative absorption spectroscopy of known concentrations of potassium permanganate solution. A laboratory exercise utilizing this approach was employed in two postsecondary institutions to simulate the forensic analysis of unknown fabric dyes to determine the identity and concentration of an unknown dye solution.

One-step synthesis and characterization of reduced graphene oxide using chemical exfoliation method

Graphene has many potential applications. Currently, its synthesis starts with the oxidation of graphite to graphene oxide followed by a separate reduction step. But, this leads to extra cost, prolonged time, and contamination. Here, we report the preparation of reduced graphene oxide (rGO) directly from graphite in a solution of potassium permanganate and concentrated sulfuric acid assisted by ultrasonication. The morphology, optical, chemical, thermal, microstructural, and electrical properties of prepared samples were studied using scanning transmission electron microscopy, selected area electron diffraction, ultra violet-visible spectroscopy, Fourier-transform infrared spectroscopy, thermogravimetric analysis, Raman spectroscopy, X-ray powder diffraction, and cyclic voltammeter, respectively. The analysis confirms that quality of rGO with thickness (∼0.36 nm) that can be dispersed in water with the desired properties was obtained. We can use this method to prepare rGO directly from graphite for energy storage.

Sergey Romanovich Mirotvortsev (1878–1949) – urologist-outstanding innovator and clinician (140th anniversary of his birth)

The main milestones of the life and creativity of Professor S.R. Mirotvortsev are described in the article. Little-known facts from the professor's biography are noted. He was rewarded with two gold medals for his students' works: “Vessels and nerves of sinews of the human foot” (with his student V.P. Vorobyov) and “Dislocations of bones of the wrist.” Notably, during the Russian-Japanese war, S.R. Mirotvortsev spent 11 months in the besieged Port Arthur and remained with the wounded who were taken prisoner by the Japanese there. From 1905 to 1914, S.R. Mirotvortsev worked in the Military Medical Academy (Saint Petersburg), initially under the leadership of Professor S.P. Fedorov and then as assistant for Professor V.A. Oppel. From 1914 until the end of his life, S.R. Mirotvortsev worked in Saratov, in the Department of General Surgery; beginning in 1920, he was a member of the fa­culty of the surgical clinic. From 1922 to 1928, S.R. Mirotvortsev was the rector of Saratov University. In the period of the Great Patriotic War, he was the chief surgeon of Saratov and the Saratov region. S.R. Mirotvortsev published approximately 150 scientific works regarding questions of clinical and field surgery. In 1908, he demonstrated the first transplantation of ureters in the rectum and defended a doctoral dissertation (1909) on this subject. Broad popularity was gained by his work regarding malignant new growths, particularly sarcomas of tubular bones. S.R. Mirotvortsev developed methods of unilateral closure of the large intestine, determined the plasticity of the sealing gland of the meninx, and processed burn wounds with potassium permanganate solution. S.R. Mirotvortsev suggested the use of the hemostatic properties of catgut for local cessation of bleeding from parenchymatous bodies and channels within the meninx. His numerous works on field surgery provided statistical analysis of 180 000 cases involving gunshot wounds; these were especially valuable during World War I (1914–1918). Notably, the Department of Faculty Surgery of the Saratov Medical Institute, as well as a street in Saratov, carries the name of S.R. Mirotvortsev. He was awarded the Labour Red Banner, the Red Star, and numerous medals.

Academician S.R. Mirotvortsev (1878 - 1949) – the Clinical Physician, the Teacher, the Urologist Innovator (to the 140 anniversary since birth)

The main milestones of life and S.R. Mirotvortsev’s creativity are given in article. The little-known facts from the biography of professor are noted. Rewarding with two gold medals for student’s works is analyzed: «Vessels and nerves of sinews of foot of the person» (in common with the student V.P. Vorobyov) and «Dislocations of bones of a wrist». It is noted that during the Russian-Japanese war spent 11 months in the besieged Port Arthur and remained with the wounded who were taken prisoner to Japanese there. From 1905 to 1914 worked in VMA at first under the leadership of professor S.P. Fedorov, then the assistant for professor V.A. Oppel. It is shown that since 1914 until the end of life worked in Saratov, occupying department of the general surgery, and since 1920 — faculty surgical clinic. From 1922 to 1928 the rector of the Saratov university. In the period of the Great Patriotic War was the chief surgeon of Saratov and the Saratov region. S.R. Mirotvortsev published about 150 scientific works on questions of clinical and field surgery. In work it is analyzed that he in 1908 offered original operation of change of ureters in a rectum, having defended the doctoral dissertation (1909) on this subject. It is noted that the broad popularity was gained by its researches about malignant new growths, in particular sarcomas of tubular bones. It developed methods of unilateral switching off of a large intestine, plasticity of a firm meninx the sealing gland, processing’s of burn wounds potassium permanganate solution. S.R. Mirotvortsev suggested to use haemostatic properties of a catgut for a local stop of bleedings from parenchymatous bodies and sine of a firm meninx. From numerous works on field surgery detailed statistical development of 180 thousand cases of a gunshot wound is especially valuable during World War I of 1914 — 1918. It is noted that the department of faculty surgery of the Saratov medical institute and the street in Saratov is called by the name of S.R. Mirotvortsev. It is awarded with awards of the Labour Red Banner, the Red Star and medals.

A simple and low-cost approach for microdistillation: Application to methanol determination in biodiesel exploiting smartphone-based digital images

Although usual in analytical chemistry, separations of volatile analytes by distillation are time-consuming, whereas gas diffusion through membranes is characterized by low efficiency. These limitations directly hinder sample throughput and sensitivity. In this work, a simple and inexpensive approach is proposed for separation of volatile analytes by microdistillation. It consists of an eppendorf type tube containing the acceptor solution suspended inside a 50 mL Falcon® capped tube containing the sample. The bottom of the sample tube is submitted to controlled heating in a water bath to promote analyte volatilization to the headspace, thus favoring its collection into the acceptor solution. The efficiency of the approach was evaluated by determination of methanol in biodiesel, whose threshold limit is 0.2% (m/m) according the current legislation. Measurements were based on discoloration of an acidic potassium permanganate solution (750 μL), caused by oxidation of methanol with consequent reduction to Mn(II). The analytical measurements were based on intensity of the reflected radiation acquired as digital images by the camera of a smartphone under controlled lighting. Values of the G channel were used as analytical parameter because of the complementarity with the color of the potassium permanganate solution. Under heating at 70 °C for 40 min, the transference of methanol to the acceptor solution was estimated at (98 ± 3)%. A linear response was achieved within 0.025% and 0.200% (m/m) methanol, with coefficient of variation (n = 10) and limit of detection (99.7% confidence level) estimated at 3.7% and 0.009% (m/m) respectively. The procedure requires only 100 μg of potassium permanganate and 200 μL of sample and generates as low as 950 μL of residue per determination. Because of the selectivity provided by microdistillation, the results obtained by the proposed approach agreed with the reference procedure at the 95% confidence level. The proposed approach is a simple, efficient, and cost-effective alternative for separation and determination of volatile species.

Elemental mercury removal over a novel starch-modified MnOx/bentonite composite

To improve the activity of elemental mercury (Hg0) removal over the bentonite supported MnOx material (MnOx/bentonite), a novel starch-modified MnOx/bentonite composite (MnOx/starch-bentonite) was synthesized for Hg0 capture. The Hg0 removal activity tested under the atmosphere of pure N2 and in the presence of O2 showed that the MnOx/starch-bentonite exhibited better activity and stability than MnOx/bentonite. It was evident that the Hg0 removal efficiency over the novel catalytic sorbent (BS10M2) only declined by 16.9%, while that over MnOx/bentonite fell by 68.7% after a 5 h test under the atmosphere of N2 + 6%O2 at 120 °C. The starch modification decreased the surface area but enhanced the surface activity of bentonite, and more activate sites were generated on the surface of bentonite after being impregnated with potassium permanganate (KMnO4) solution. The improved Hg0 removal performance was probably due to the generation of more Mn4+ and the newly introduced ester groups formed by the partial oxidation reaction between starch and KMnO4 on the support, which played essential roles in mercury catalytic oxidation and adsorption. The MnOx were generated in two ways: KMnO4 decomposition in a neutral solution condition and KMnO4 reduction by the starch with hydroxyl groups.

Automatic determination of arsenate in drinking water by flow analysis with dual membrane-based separation

The sequential application of a polymer inclusion membrane (PIM), composed of poly(vinylidenefluoride-co-hexafluoropropylene) and the anionic extractant Aliquat 336, and a microporous polytetrafluoroethylene (PTFE) gas-permeable membrane was utilized for the first time to develop a flow analysis (FA) system, for the automatic determination of trace levels of arsenate (As(V)) in drinking water as arsine. The system incorporated a flow-through extraction cell for separation and preconcentration of arsenate and a gas-diffusion cell for the separation of arsine prior to its spectrophotometric determination based on the discoloration of a potassium permanganate solution. Under optimal conditions the FA system is characterized by a limit of detection of 3.0 μg L−1 As(V) and repeatability of 1.8% (n = 5, 25 μg L−1 As(V)) and 2.8% (n = 5, 50 μg L−1 As(V)). The newly developed FA method was successfully applied to the determination of arsenate in drinking water samples in the μg L−1 concentration range.

Construction of TiO2 Nanotubes/C/MnO2 Composite Films as a Binder-Free Electrode for a High-Performance Supercapacitor.

Although titanium dioxide (TiO2) exhibits excellent promise in electrode materials for supercapacitors, its poor conductivity and low areal specific capacitance hamper further development. In this work, we have designed a clever way to deposit manganese dioxide (MnO2) in order to improve its electrochemical performance via a facile and typical hydrothermal method. In a hydrothermal process, carbon (C), which deposited via new gas thermal penetration, acts as a reducing agent, while a potassium permanganate (KMnO4) solution acts as an oxidant. In this way, MnO2, which has a high theoretical capacity, is generated on TiO2 nanotube arrays (denoted as TNTs) successfully. Remarkably, a TNTs/C/MnO2 film prepared at a hydrothermal temperature of 90 °C and 0.3 g of KMnO4 revealed a superior electrochemical property with 55 mF/cm2 areal capacitance at a scan rate of 5 mV/s, 23 times more enhanced than that of a TNTs/C film. Also, the energy density of a TNTs/C/MnO2 film reached 46.8 Wh/cm2 when the power density was 0.12 mW/cm2, and the energy density still remained at 22.4 Wh/cm2 at a high power density of 0.8 mW/cm2. After 1000 cycle tests, 93.2% capacitance was still retained, indicating excellent reversibility and cycle stability of TNTs/C/MnO2 electrode. This work opens up a facile path for efficient growth of electrode materials with high performance for energy storage devices.

OXIDATIVE-REDUCING PROCESSES WITH PARTICIPATION OF MANGANESE IONS INITIATED BY AN ELECTRIC DISCHARGE IN AQUEOUS SOLUTION

The results of experimental studies of the kinetics of oxidation-reduction of Mn7+ ions (MnO4-) in aqueous solutions initiated by the action of a discharge of a direct current of atmospheric pressure in air are analyzed in the article. A solution of potassium permanganate served as a discharge cathode. The range of initial solution concentrations for Mn7+ ions was (0.44-2.5) mmol/l, and discharge currents (20-60) mA. It was found that the discharge action leads to the reduction of Mn7+ ions and discoloration of the solution. At the same time, dark solid particles with a size of 0.1 μm to 20 μm are formed. X-ray diffraction analysis showed that the particles are amorphous, and energy dispersive X-ray analysis showed that the powder is manganese oxide (IV). The kinetics of reduction-oxidation of Mn7+ ions is measured. It is shown that the obtained data on the kinetics of the reduction of Mn7+ ions in the best way (the determination coefficient R2≈0.99) can be described by the scheme X↔Y↔Z, where X is the starting material, and Y and Z are the reaction products. The processing of kinetic curves on the basis of this scheme found the effective rate constants of the corresponding stages. It was found that the effective rate constants depend on the initial concentration of the solution. At a discharge current of 20 mA, an increase in the concentration from 0.44 to 2.5 mol/l led to a decrease in the rate constant for the reduction of Mn7+ ions from (2.48 ± 0.5) ·10-2 to (7.2 ± 1.5) ·10-3 s-1, respectively. Possible mechanisms of processes are discussed. It is assumed that the main particles involved in the oxidation reactions of the reduction of manganese ions are H2O2, HO2, OH and solvated electrons that are formed in the solution under the action of a discharge.
 For citation:
 Shutov D.A., Sungurova A.V., Smirnova K.V., Manukyan A.S., Rybkin V.V. Oxidative-reducing processes with participation of manganese ions initiated by electric discharge in aqueous solution. Izv. Vyssh. Uchebn. Zaved. Khim. Khim. Tekhnol. 2018. V. 61. N 9-10. P. 23-29

Towards understanding the morphological, magnetic, optical and electrical properties of MnO2 nanowires for magneto-and optoelectronic applications

Mixed solutions of manganese sulfate and potassium permanganate were hydrothermally used to synthesis α-MnO2 nanowires. Energy dispersive analysis of X-ray revealed the formation of stoichiometric MnO2 and X-ray diffraction revealed the formation of single crystalline α-MnO2. Examinations using both SEM and TEM yielded nanowires with diameters ranged from 40 to 50 nm and lengths ranged from 5 to 6 µm. The magnetic properties revealed that the α-MnO2 NWs exhibit ferromagnetic/antiferromagnetic characteristics at liquid nitrogen and room temperatures. This kind of materials may be find usage in electromagnetic shielding applications. Direct optical band gap of 2.6 eV, that was blue shifted from the bulk value, was reported. Photoluminescence examinations displayed a strong emission peak around 395 nm and broad peak around 477 nm. The α-MnO2 NWs showed a semiconducting behavior where the resistivity decreased with increasing temperature. Two activation energies were reported; the low temperature activation energy was 0.089 eV and the high temperature activation energy was 0.782 eV. Based on the observed high intensity UV emission peak, the obtained α-MnO2 NWs may find applications in UV light emitting diodes.

A METHODOLOGICAL APPROACH TO DESIGNING EXPERIMENTS WHEN DEALING WITH IDENTIFICATION TESTS FOR MEDICINAL PRODUCT COMPONENTS (AS ILLUSTRATED BY ASCORBIC ACID)

The need for identification testing of active substances or excipients in multi-component medicinal products, including the use of qualitative tests, calls for research substantiating the choice of tests and test conditions with due regard to interference effects caused by other components of medicinal products and the amount of the sample used. The aim of the study was to develop a methodological approach to designing experiments while selecting qualitative reactions for identification testing of a medicinal product component based on the results of studies investigating the possibility of using known qualitative tests (as illustrated by ascorbic acid in a multi-component product — 0.4 mg of ascorbic acid per 100 mg of the vial contents) with due regard to interference on the part of other medicinal product components and the amount of the sample used. Material and methods: the study focused on a multi-component medicinal product — lyophilisate for solution for intravenous and intramuscular injections containing an antiinflammatory active substance and ascorbic acid as a stabilizing agent (antioxidant). The analysis of literature sources helped to determine qualitative tests that were assessed for potential use for identification testing of ascorbic acid as a component of the analysed medicinal product. The study involved experimental testing of the qualitative reactions based on acidic and reducing properties of ascorbic acid. Results: it was demonstrated that several well-known qualitative tests could be used for identification testing of ascorbic acid as a component of the analysed medicinal product, namely, the reaction of ferrous ascorbate formation and the reaction of silver nitrate reduction to metallic silver after preliminary separation of ascorbic acid from the other medicinal product components, as well as the reaction of Prussian blue formation, iodine test and reaction with a potassium permanganate solution, which do not require additional sample preparation. It is not practicable to use the reaction with a methylene blue solution and the Fehling’s reagent reaction for this particular medicinal product, since their results are feeble. Conclusions: the analysis of the multi-component medicinal product helped to develop a methodological approach to choosing qualitative reactions for identification testing of one of the medicinal product’s components (e.g., ascorbic acid). The suggested algorithm includes the choice of reactions, determination of their sensitivity and applicability for a particular medicinal product, analysis of the other components’ effects on the results of the chemical reaction, and the need for additional sample preparation. The whole complex of the studies performed helped to determine qualitative reactions and optimal conditions for identification testing of the analysed substance.